CN113080866A

CN113080866A - Tongue picture acquisition device and method for experimental animals

Info

Publication number: CN113080866A
Application number: CN202110384533.4A
Authority: CN
Inventors: 陈清光; 陆灏; 许家佗; 安百芝; 张晴; 徐隽斐; 姚政; 龚凡; 韩煦; 蔡梦洁; 闫子慧; 金燊懿; 刘亚华
Original assignee: Shuguang Hospital Affiliated to Shanghai University of TCM
Current assignee: Shuguang Hospital Affiliated to Shanghai University of TCM
Priority date: 2021-04-09
Filing date: 2021-04-09
Publication date: 2021-07-09

Abstract

The invention relates to a tongue image collection device and method for experimental animals. The device is provided with a box body, a computer and a display. The box body is provided with a test table, a mouth opening clip that stretches the upper and lower teeth and has an adjustable angle, a standard light source, and is used for photographing the front image and two side images of the tongue. an image acquisition device and a touch rod for the sublingual vein; the computer is provided with an image acquisition module, an image processing module, an image comparison module and an image output module for receiving images, calculating the frontal area of the tongue body, the lateral periphery of the tongue body Track, compare the frontal area of the tongue between different individuals and the images whose screening difference exceeds the threshold value, and compare the peripheral tracks of the two sides of the tongue of the same individual and the images whose screening deviation exceeds the threshold value; the display is used to display the output image and results. The invention helps to obtain accurate and objective experimental results in animal experiments of tongue images, scientifically evaluate the relationship between tongue images and diseases, and further guide clinical tongue diagnosis.

Description

Tongue picture acquisition device and method for experimental animals

Technical Field

The invention relates to the field of experimental instruments, in particular to a tongue picture acquisition device and method for experimental animals.

Background

The tongue diagnosis is a method of observing the changes of the tongue proper and tongue coating of a patient to diagnose the disease, and is one of the features of the diagnosis methods of traditional Chinese medicine. The tongue proper can reflect the deficiency and excess of the zang-fu organs and the abundance or insufficiency of qi, blood and body fluids; the tongue coating reflects the pathogenic cold and heat, and the pathogenic healthy qi waning and growing. Similarly, the tongue is the miniature of the animal body, and the visceral status, the abundance or insufficiency of the material energy, the changes, the circulation, the diseases and the disease root of the animal body can be reflected on the tongue. The animal is taken as a model to research the tongue picture of the animal, and the method plays an important role in disclosing the rich experience of Chinese medical science and establishing a scientific complete theoretical system of the Chinese medical science.

Patent document CN211560070U, publication No. 2020.09.25, discloses a tongue image information collecting and analyzing device, which includes a moving assembly, a protective assembly and a tongue image instrument main body, where the moving assembly includes a base plate and a first linear guide rail, the first linear guide rail is installed on one side of the base plate, the protective assembly includes a sliding plate, a frame, a support plate, a shaft rod and a protective film, one side of the frame is fixed on the surface of the base plate, and the sliding plate is installed inside the frame in a sliding manner.

Patent document CN201814562U, bulletin date 2011.05.04, discloses a portable handheld tongue picture collecting device for collecting information of traditional chinese medical inspection, which comprises a detachable collecting ring, a UV mirror, an LED light source, a camera module, a handle, a circuit board, a telescopic cylinder and a control panel, wherein the detachable collecting ring is fixedly connected with the outer surface of the front end of the telescopic cylinder, the UV mirror is fixedly connected with the front end of the inner surface of the telescopic cylinder, the LED light source is fixedly connected with the middle end of the inner surface of the telescopic cylinder, the camera module is fixedly connected with the rear end of the inner surface of the telescopic cylinder, the control panel is fixedly connected with the camera module, the handle is fixedly connected with the rear lower end of the telescopic cylinder, and the circuit board is.

Patent document CN203609537U, bulletin day 2014.05.28 discloses a tongue picture observation mouse board, including the objective table, be rectangular array on the objective table and be provided with four fixed posts, be provided with the recess in the place ahead corresponding to four fixed posts on the objective table, the recess both sides all are provided with the facing pillar, and two facing pillar upper ends all are provided with the rubber band, and two rubber bands link to each other with the facing.

However, the tongue picture collection is interfered by a plurality of factors, so that the collection result is unstable, the data comparability is poor, and the conclusion of a correct experiment is influenced. Therefore, a tongue picture collecting device for experimental animals is needed to improve the accuracy of the experiment.

Disclosure of Invention

The invention aims to provide a tongue picture acquisition device for experimental animals aiming at the defects in the prior art.

Still another object of the present invention is to provide a tongue picture collecting method for experimental animals.

In order to achieve the first purpose, the invention adopts the technical scheme that:

a tongue picture collecting device aiming at experimental animals comprises a box body, a computer and a display;

an experiment table is arranged in the box body, and a limb fixing component and a mouth opening clamp are arranged on the experiment table;

the box body is also internally provided with a standard light source, a first image acquisition device for shooting the front image of the tongue body, and a second image acquisition device and a third image acquisition device for shooting the left side surface and the right side surface of the tongue body;

the computer is provided with an image acquisition module, an image processing module, an image comparison module and an image output module;

the image acquisition module is used for receiving the shot tongue body front image and the two tongue body side images and transmitting the images to the image processing module;

the image processing module comprises an area calculating unit and a track generating unit, wherein the area calculating unit is used for calculating the area of the tongue body according to the front image of the tongue body, and the track generating unit is used for respectively drawing the peripheral tracks of the left side surface and the right side surface of the tongue body according to the side image of the tongue body;

the image comparison module is used for comparing the front areas of the tongues of different individuals and screening out images with a difference value exceeding a threshold value with the average value; the image processing method is also used for comparing peripheral tracks of the left side surface and the right side surface of the tongue body of the same individual and screening out images with deviation exceeding a threshold value;

the image output module is used for outputting images and calculation results to a display.

As a preferred example of the present invention, the open-mouth clip comprises an arc-shaped upper clip body and a lower clip body; the two ends of the upper clamp body are respectively provided with a pawl, the two ends of the lower clamp body are respectively provided with a turntable, the periphery of the turntable is provided with a ratchet, and the center of the turntable is respectively connected with a transverse rotating rod; the two end parts of the upper clamp body are rotatably connected with the rotating rods at the two end parts of the lower clamp body.

More preferably, the laboratory bench is equipped with the mouth of opening and presss from both sides the support, the mouth of opening presss from both sides the support and includes first perpendicular pole and the perpendicular pole of second, and two dwangs run through respectively rather than rotate the first perpendicular pole of being connected and the perpendicular pole of second, and the through-hole of rethread box curb plate is outside until the box, the dwang is equipped with the knob at the outside tip of box.

More preferably, the upper and lower clamp bodies are arcuate in cross section in the sagittal plane.

As another preferable example of the present invention, an elliptical head fixing groove is provided on the experiment table.

In another preferred embodiment of the present invention, a touch bar is suspended above the open mouth clamp, and the touch bar is driven by a motor located outside the box body to vertically move up and down.

As another preferable example of the present invention, a fourth image capturing device is disposed in the box body, and is configured to capture a sublingual choroid image.

As another preferred example of the invention, the limb fixing component is four upright posts or rubber bands.

In order to achieve the second object, the invention adopts the technical scheme that:

a tongue picture collecting method aiming at experimental animals is implemented on the basis of the tongue picture collecting device.

The invention has the advantages that:

1. according to the invention, through the evaluation of the front area of the tongue body and the tracks of the two side surfaces of the tongue body, unqualified tongue body images are screened out, the deviation of experimental results caused by a plurality of factors in the acquisition process of the tongue body images is avoided, the accurate and objective experimental results are ensured to be obtained, the association between the tongue manifestation and the disease is scientifically evaluated, and the clinical tongue diagnosis is further guided.

2. The mouth opening clamp provided by the invention can be used for spreading the animal teeth from the upper direction and the lower direction to expose the tongue body, so that each position of the animal tongue body can be observed, and a more comprehensive tongue image can be obtained.

3. The open mouth clamp is provided with the angle adjusting device, the overall structure is simple, the adjusting operation is simple and convenient, and the exposure condition of the animal tongue body can be adjusted rapidly in an experiment.

4. The invention can collect the sublingual venation to obtain more complete tongue picture.

5. The device is provided with the touch rod, so that whether the tongue body is in a natural relaxation state can be verified before the image is collected, and the collected image can obtain an objective result.

In general, the method is beneficial to conveniently collecting the tongue picture of the experimental animal to obtain the tongue body image of the standard animal, avoids the interference of various factors on the experimental result, and obviously improves the accuracy and objectivity of tongue picture research.

Drawings

FIG. 1 is a schematic view showing the construction of a tongue image collecting apparatus for an experimental animal according to example 1.

FIG. 2 is a schematic structural view of the case of embodiment 1.

Figure 3 is a schematic view of the construction of the open mouth clip.

Figure 4 is a cross-sectional schematic view of an open-mouth clip.

Fig. 5 is a block diagram of a computer.

FIG. 6 is a schematic structural view of a case of embodiment 2.

Detailed Description

The following detailed description of the present invention will be made with reference to the accompanying drawings.

The reference numerals and components referred to in the drawings are as follows:

1. box body	2. Experiment table
		3. Limb fixing component	4. Head fixing groove
5. Open mouth clamp	51. Upper clamp body
		52. Lower clamp	511. Pawl
521. Rotary disc	522. Ratchet tooth
		523. Rotating rod	524. Rotary knob
6. Open mouth clamp bracket	61. First vertical rod
		62. Second vertical rod	7. Standard light source
81. First image acquisition device	82. Second image acquisition device
		83. Third image acquisition device	9. Computer with a memory card
91. Image acquisition module	92. Image processing module
		921. Area calculation unit	922. Trajectory generation unit
93. Image comparison module	94. Image output module
		10. Display device	11. Touch rod
12. Electric machine

Example 1

Referring to fig. 1, fig. 1 is a schematic structural view of a tongue image collecting device for experimental animals in example 1. The tongue picture collecting device is provided with a box body 1, a computer 9 and a display 10.

Referring to fig. 2, fig. 2 is a schematic structural diagram of the box body of embodiment 1. The utility model discloses a four-column. A mouth opening clamp 5 is arranged right above the head fixing groove 4.

Referring to fig. 3, fig. 3 is a schematic structural view of the open-mouth clip. The mouth opening clamp 5 comprises an upper clamp body 51 and a lower clamp body 52. The upper clamp body 51 and the lower clamp body 52 are both arc-shaped and are consistent with the arrangement direction of the teeth of the experimental animal. The two ends of the upper clamp body 51 are respectively provided with a pawl 511, the two ends of the lower clamp body 52 are respectively fixedly provided with a rotating disc 521, the periphery of the rotating disc 521 is provided with a ratchet 522, and the center of the rotating disc 521 is respectively connected with a transverse rotating rod 523. The two ends of the upper clamp body 51 are rotatably connected to the rotating rods 523 at the two ends of the lower clamp body 52.

Referring to fig. 4, fig. 4 is a cross-sectional view of the open-mouth clip. The upper and

lower clamp bodies

51 and 52 are curved in cross section in the sagittal plane.

Referring to fig. 2 again, the experiment table 2 is provided with a mouth opening clamp support 6, the mouth opening clamp support 6 includes a first vertical rod 61 and a second vertical rod 62, the first vertical rod 61 and the second vertical rod 62 are respectively located at two sides of the head fixing groove 4, two rotating rods 523 respectively penetrate through the first vertical rod 61 and the second vertical rod 62 which are rotatably connected with the two rotating rods and then reach the outside of the box body 1 through a through hole of a side plate of the box body 1, and a knob 524 is arranged at the end of the rotating rod 523 outside the box body 1. A standard light source 7 is arranged in the box body 1, and a first image acquisition device 81 is arranged on a side plate right in front of the mouth opening clamp 5. And a second image acquisition device 82 and a third image acquisition device 83 are arranged on the side plates on the left side and the right side of the head fixing groove 4. The first image acquisition device 81, the second image acquisition device 82 and the third image acquisition device 83 are all connected with the computer 9.

Referring to fig. 5, fig. 5 is a block diagram of a computer. The computer 9 is provided with an image acquisition module 91, an image processing module 92, an image comparison module 93 and an image output module 94. The image acquisition module 91 is used for acquiring tongue image data of the experimental animal in the box body 1 in real time, specifically comprises a tongue front view and two tongue side views which are respectively shot by the first image acquisition device 81, the second image acquisition device 82 and the third image acquisition device 83, and transmits the tongue front view and the two tongue side views to the image processing module 92. The image processing module 92 includes an area calculating unit 921 and a trajectory generating unit 922, the area calculating unit 921 is configured to identify a tongue body according to a color and a shape of the tongue body in the front view of the tongue body and calculate an area of the tongue body, and the trajectory generating unit 922 is configured to identify the tongue body according to the color and the shape of the tongue body in the two side views of the tongue body and respectively draw a peripheral trajectory of the tongue body. The image comparison module 93 has two functions, one is to compare the tongue areas in the tongue frontal views of different animal individuals, calculate an average value, and eliminate the image whose difference value with the average value exceeds a threshold (e.g. 10% of the average value); the other function is to compare the peripheral tracks of the left and right sides of the tongue body of the same animal individual, judge the track coverage degree, and reject the image in which the area of the non-overlapped part of the peripheral tracks of the left and right sides of the tongue body exceeds the threshold (for example, 20% of the average value of the peripheral tracks of the left and right sides of the tongue body). The image output module 94 is used for outputting the photographed tongue image and the result processed by the image processing module 92, including the calculated area and the generated peripheral track of the side surface of the tongue, to the display 10 for display.

The invention relates to a tongue picture collecting device for experimental animals, which comprises the following steps: in the process of collecting the tongue picture of the experimental animal, firstly, the experimental animal such as a rat is anesthetized, the anesthetized rat is placed on the experiment table 2, four limbs are respectively fixed on the limb fixing component 3, and the head is placed in the head fixing groove 4 to form a prone position. The mouth opening clamp 5 is placed in the mouth of a rat, the upper clamp body 51 and the lower clamp body 52 respectively support the upper tooth surface and the lower tooth surface of the rat, the rotating rod 523 is rotated through the knob 524 to adjust the opening degree of the mouth cavity of the rat to a proper size so as to fully expose the tongue, then the box body 1 is closed, the first image acquisition device 81, the second image acquisition device 82 and the third image acquisition device 83 are started to respectively shoot to obtain a front image of the tongue and two side images of the tongue, the images are acquired by the image acquisition module 91 and transmitted to the image processing module 92, the image processing module 92 identifies the front image and the side images, the tongue is identified according to color and shape and calculated to obtain the area of the tongue for the front image, the tongue is identified according to color and shape and draws the peripheral track of the tongue for the side images, and then the result is output to the image comparison module 93. The image comparison module 93 obtains tongue image results from different rats, calculates the area average value of the front faces of the tongues from different rats, and then screens out images with errors exceeding a threshold value from the average value; meanwhile, the image comparison module 93 obtains peripheral tracks of the left and right sides of the tongue body from the same rat, compares the two peripheral tracks, and screens out the two images when the area of the non-overlapped part exceeds a threshold value. During the experiment, the acquired original image of the animal tongue body, the processed result of the tongue body area, the peripheral track and the like can be watched through the display 10, and the identification of the qualified or unqualified image can also be watched. The resulting images were finally screened for further tongue analysis.

For the present invention, it should be noted that: animal experiment research on tongue manifestation can help clear the relationship between disease and tongue manifestation, further guide the diagnosis of tongue manifestation in clinical practice, and improve the objective level of tongue diagnosis. However, the acquisition of the tongue picture is easily interfered by various factors to cause unqualified acquired images, and the unqualified images are used as data to obtain an experimental conclusion, so that the accuracy of the experimental conclusion is seriously interfered, and the research value is reduced. The inventor adds the image processing module 92 in the experimental animal tongue picture acquisition device, and rejects the image in which the tongue body area deviates from the average value remarkably by comparing the tongue body front area among different animal individuals, because the weights of the commonly selected experimental animals are basically consistent, if the weight difference value is not more than 10%, under general conditions, the tongue body area in the tongue body front image should not deviate too much, otherwise, the tongue body is not fully exposed, the tongue body is not fully extended, the tongue body is curled, and the like, which all affect the correct judgment of the tongue picture, and if the tongue body is not fully extended, the tongue body is curled, which affects the qi and blood running of the tongue and causes the tongue color change; the peripheral tracks of the tongue bodies on the left side and the right side of the same animal individual are compared, and when the difference is large, the situation that the tongue bodies are not completely flat or not relaxed is indicated. According to the method, the risk of result errors caused by various factors such as insufficient exposure and incomplete extension of the tongue body of the experimental animal, improper angle between the tongue body and the camera and the like is greatly reduced, and the method is favorable for obtaining accurate and objective tongue picture research results and correctly guiding clinical application. In order to facilitate the exposure of the tongue body, the open-mouth clamp 5 is designed to comprise an upper clamp body and a lower clamp body, so that various parts of the tongue body of the animal, including the tongue tip, the tongue margin and the like, can be more fully exposed and observed, and a more comprehensive tongue picture result can be obtained. The upper clamp body 51 and the lower clamp body 52 are respectively in the same radian of the animal teeth, which is beneficial to the stable fixation with the animal teeth, and the sectional pictures of the sagittal planes of the upper clamp body and the lower clamp body are also in the shape of an arc, which is also beneficial to strengthening the stable fixation of the mouth opening clamp 5 and preventing the mouth opening clamp from falling off from the teeth. The angle of the upper clamp body and the lower clamp body of the open mouth clamp 5 is adjusted through the ratchet 522 and the pawl 511, the structure is simple, the operation is easy, the opening angle can be accurately adjusted, and the flexibility of the experiment is improved. The head fixing groove 4 is designed into an oval groove, so that the experimental animal can conveniently form a prone position to expose the tongue body, the general position of the tongue body can be marked, and the experimental animal can be conveniently prevented by an experimenter. The limb fixing component 3 is used for fixing animal limbs, is not limited to the structure of a stand column, and can also be a fixing component such as a rubber band. The standard light source 7, i.e. a light source capable of emitting a stable light intensity and brightness, is composed of a plurality of lamp caps, so that no shadow exists at the tongue body. The image capture device may be Eolane a 12. The standard light source and the image acquisition device are integrated in the box body. The standard light source and image acquisition environment are as follows: a cold white LED lamp (KA-3021HVR4D1Z1SC 1-SH); the color temperature is 6466K; the illumination intensity is 2354 lux; in the M (manual) mode, the diaphragm 13 and the shutter 1/60S are used.

Example 2

The tongue image collecting device of the present embodiment has basically the same structure as the tongue image collecting device of embodiment 1, except that: referring to fig. 6, fig. 6 is a schematic structural diagram of the case in embodiment 2. A touch rod 11 is also hung above the mouth opening clamp 5, and the touch rod 11 is driven by a motor 12 positioned outside the box body 1 and can vertically lift. The touch rod 11 is used for judging the relaxation state of the tongue body of the experimental animal in a mode of touching the tongue body of the experimental animal after the experimental animal is fixed and before the tongue body image is collected, and if the tongue body is completely relaxed, the tongue body does not have any reaction after being touched, so that the image can be shot.

Example 3

The tongue image collecting device of the present embodiment has basically the same structure as the tongue image collecting device of embodiment 1, except that: a fourth image acquisition device is also hung at the tail part of the mouth opening clamp 5 and is used for acquiring sublingual veins so as to obtain more comprehensive tongue image results.

The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and additions can be made without departing from the method of the present invention, and these modifications and additions should also be regarded as the protection scope of the present invention.

Claims

1. a tongue image collection device for experimental animals, is characterized in that, comprises box body, computer and display;

An experiment table is arranged in the box, and a limb fixing assembly and a mouth opening clip are arranged on the experiment table;

The box is also provided with a standard light source, a first image acquisition device for photographing the frontal image of the tongue, and a second image acquisition device and a third image acquisition device for photographing the left and right sides of the tongue;

The image acquisition module is used to receive the frontal image of the tongue and the two lateral images of the tongue, and transmit them to the image processing module;

The image processing module includes an area calculation unit and a trajectory generation unit, the area calculation unit is used to calculate the area of the tongue body according to the frontal image of the tongue body, and the trajectory generation unit is used to respectively outline the tongue body according to the side image of the tongue body. The peripheral trajectory of the left and right sides;

The image comparison module is used to compare the frontal area of the tongue of different individuals, and to screen out images whose difference from the average value exceeds a threshold; it is also used to compare the peripheral trajectories of the left and right sides of the tongue of the same individual, and to screen out those whose deviation exceeds the threshold. image;

The image output module is used for outputting images and calculation results to the display.

2 . The tongue image collection device according to claim 1 , wherein the mouth-opening clip comprises an arc-shaped upper clip body and a lower clip body; two ends of the upper clip body are each provided with a pawl. 3 . , the two ends of the lower clip body are each provided with a turntable, the outer periphery of the turntable is provided with ratchet teeth, and the center of the turntable is connected with a horizontal rotating rod; the two ends of the upper clip body are connected to the lower clip body. The rotating rods at the two ends are rotatably connected.

3 . The tongue image collection device according to claim 2 , wherein the test bench is provided with a mouth-opening clip bracket, and the mouth-opening clip bracket comprises a first vertical rod and a second vertical rod, and two rotating rods. 4 . The first vertical rod and the second vertical rod which are rotatably connected with the rotating rod respectively pass through the through hole of the side plate of the box body to the outside of the box body, and the end of the rotating rod outside the box body is provided with a knob.

4 . The tongue image collecting device according to claim 2 , wherein the cross-sections of the upper clip body and the lower clip body on the sagittal plane are arc-shaped. 5 .

5 . The tongue image collecting device according to claim 1 , wherein an oval head fixing groove is provided on the test table. 6 .

6 . The tongue image collecting device according to claim 1 , wherein a touch rod is suspended above the mouth opening clip, and the touch rod is driven by a motor located outside the box to realize vertical lifting. 7 .

7 . The tongue image collecting device according to claim 1 , wherein a fourth image collecting device is provided in the box, which is used for collecting images of sublingual venation. 8 .

8 . The tongue image collection device according to claim 1 , wherein the limb fixing components are four uprights or rubber rings. 9 .

9 . A method for collecting tongue images for experimental animals, wherein the method for collecting tongue images is implemented based on the device for collecting tongue images according to any one of claims 1 to 8 .