CN112868919B - A feed for improving the quality of ram semen and its application - Google Patents

Abstract

The present invention specifically provides a feed for improving the quality of ram semen and its application. The basic diet is used as a raw material, and appropriate L-citrulline is used as an addition. The results of the feeding test showed that the contents of reproductive hormones GnRH, LH, FSH, T, and _E2 in ram serum and semen were all increased, and the antioxidant substances CAT, MDA, SOD, The content of T‑A, GSH‑PX, NO and ASAFR has been improved, and the sperm density in semen, the number of linear motion sperm, the viability rate have been increased, and the mortality rate and deformity rate have been significantly reduced. It has wide practicability for the field of ram breeding and huge potential development value.

Description

A feed for improving the quality of ram semen and its application

technical field

The invention belongs to the technical field of animal reproductive physiology and reproduction, relates to a feed and its application, in particular to the technical field of a feed for improving the quality of ram semen and its application.

Background technique

The price of mutton continued to rise rapidly, and the overall consumption demand showed an accelerated growth trend, and the total supply exceeded demand. It is the general trend to further accelerate the development of the mutton sheep industry. Improving the quality of sheep semen is one of the important measures to expand the breeding scale of mutton sheep in the short term. The semen quality of rams mainly depends on genetic characteristics, feeding management and semen collection techniques. Therefore, breeding management measures for rams are very important. The poor quality of ram semen determines whether it can be used for insemination and whether it can meet the standards required for insemination of ewes. The quality of semen will be related to the production of frozen semen, the conception rate of ewes to be bred and the economic benefits of farmers.

In the existing technical schemes for improving the semen quality of rams, the method of compound feed ingredients of Chinese herbal medicine is mostly used. The invention patent with the application number of 201310718631.2 provides a feed for improving the semen quality of sheep, including spinach, rehmannia glutinosa, Euonymus chinensis, Soybean oil, licorice, wheat bran, black bone Zouma, Ophiopogon japonicus, yeast powder, honeysuckle, rice bran, soybean meal, shrimp roe flower, shegan, Chinese cabbage, skullcap, salt, lysine, poria cocos, threonine and glucose; the application number is The invention patent of 201711245024.3 provides a feed additive to improve the reproductive ability and semen quality of male animals. The raw materials include epimedium, cistanche, eucommia, actinolite, vitamins, L-arginine, copper glycinate, Hepu pearl oyster ; The invention patent with the application number 201710733905.3 is a preparation method of feed to improve the quality of sheep semen, which can include: spinach, rehmannia glutinosa, euonymus chinensis, soybean oil, licorice, wheat bran, black bone Zouma, Ophiopogon japonicus, yeast powder, honeysuckle , rice bran, soybean meal, shrimp roe flower, shegan, cabbage, baicalin, salt, lysine, poria cocos, threonine and glucose. Although the addition of natural plant ingredients is used, the addition of many Chinese herbal medicine ingredients also has certain disadvantages. First, there are problems of high cost and unstable quality of Chinese herbal medicine raw materials in the acquisition of raw materials, and some Chinese herbal medicine ingredients. The interaction relationship is not fully studied, and whether it has adverse effects needs further study.

There are also some technical solutions that use separate additions in the basic diet. For example, the invention patent with application number 201811086252.5 provides a method of using chestnut polysaccharides to improve the quality of animal semen. Male mammals pass through daily after weaning. Supplement chestnut polysaccharides by gavage or adding to feed. The intake dose of small male mammals is 0.1-100mg/kg body weight, and the intake dose of medium-sized male mammals is 0.5-200mg/kg feed. The invention patent application number is 201610803530.9 Provided in the invention is a method for improving the quality of sheep semen, which is characterized in that: making rams take cysteamine orally, and the ratio of the daily dose taken to the body weight of rams is 9.0-15.0 mg/kg. However, there is a need for a large amount of feeding, the cost is high in actual feeding management, and the economic benefits of breeding cannot be highlighted.

Contents of the invention

Aiming at the deficiencies in the technical solutions for improving the quality of ram semen in the prior art, such as complex components, unstable quality control and high cost in the actual breeding process, the purpose of the present invention is to provide a feed and a feed for improving the quality of ram semen Its application has a significant impact on the levels of reproductive hormones GnRH, LH, FSH, T, and _E2 in ram serum and semen, and significantly improves the antioxidant substances CAT, MDA, SOD, TA, GSH-P _X , NO, ASAFR content, ejaculation volume, density, dead sperm count, mortality rate, abnormal sperm count, deformity rate, linear motility sperm count, and motility rate in semen quality have been improved to varying degrees. It has wide practicability and huge potential development value for the field of ram breeding.

The present invention is realized through the following technical solutions:

The invention provides a feed for improving the quality of ram semen, which is obtained by mixing a basic ration with L-citrulline, wherein each part of the basic ration uses 400 kg of wheat straw, 220 kg of green grass, 430 kg of alfalfa, and 100 kg of Dabeinong 702 , 186,300kg of fattening fertilizer, 13kg of baking soda, and 9kg of animal husbandry salt are mixed, and the L-citrulline is added according to 2-6mg/piece.

Preferably, the addition of L-citrulline is 6mg/piece.

Preferably, the dry matter content of the basal ration is 1.26%.

Preferably, the moisture content of the basal diet is 41%.

Further, the present invention specifically provides an application of a feed for improving the quality of ram semen in feeding for improving the quality of ram semen.

The feeding amount is 0.69kg/only, and the feeding frequency is twice a day.

The "Dabeinong 702" used in the present invention is the mutton sheep compound feed 702 produced by Xinjiang Dabei Agriculture and Animal Husbandry Science and Technology Co., Ltd., and the "Fattening Material 186" used in the present invention is Chia Tai-186 produced by Shijiazhuang Zhengda Co., Ltd. Concentrated feed for mutton sheep can be purchased by the general public through public channels.

By implementing the specific content of the invention of the present invention, the following beneficial effects can be achieved:

(1) A feed for improving the semen quality of rams provided by the present invention, which is obtained by adopting the basal diet and adding L-citrulline in an appropriate proportion to improve the semen quality of rams, obtained by adopting the technical scheme provided by the present invention The cost of feed to improve the quality of ram semen is low, and the daily cost of each sheep is only 2.65 yuan, which can significantly reduce the cost in the breeding process, and has wide practicability for the field of ram breeding.

( ₂ ) By adopting a feed that improves the quality of ram semen provided by the present invention, the levels of reproductive hormones GnRH, LH, FSH, T, and E in ram serum and semen can be significantly improved, and the levels of ram serum and semen can be significantly improved. The contents of CAT, MDA, SOD, TA, GSH-P _X , NO, and ASAFR in the serum have antioxidant capacity.

(3) by adopting a kind of feed that improves the quality of ram semen provided by the present invention, ejaculation amount, density, dead sperm count, mortality rate, deformed sperm count, deformity rate, linear motion sperm count, live rate in the ram semen quality All have different degrees of improvement and improvement, and have huge potential development value for the field of ram breeding.

Description of drawings

none

detailed description

Hereinafter, the present invention will be described with reference to examples, but the present invention is not limited to the following examples.

Materials in the present invention include: wheat straw, green grass, alfalfa, Dabeinong 702, fertilizer 186, corn, baking soda, animal husbandry salt, vitamin AD3, sodium selenite VE, L-citrulline (purchased from Shandong Pingju Biological Co., Ltd. Science and Technology Co., Ltd., purity 99.1%), "Dabeinong 702" is the mutton sheep compound feed 702 produced by Xinjiang Dabeinong Animal Husbandry Technology Co., Ltd., and the "Fattening Material 186" used in the present invention is produced by Shijiazhuang Zhengda Co., Ltd. Zhengda-186 mutton sheep concentrated feed, distilled water, normal saline, blood cell counting board, microscope, disposable blood collection needle, sodium citrate anticoagulant tube, antioxidant index in plasma and plasma hormone level were entrusted to Nanjing Jiancheng Institute of Bioengineering to adopt kit assay. The reagents and materials can be purchased through public channels, and the equipment and instruments used in the process are common equipment in this field.

All materials, reagents and instruments selected in the present invention are well known in the art, but do not limit the implementation of the present invention, and some other reagents and equipment well known in the art are applicable to the implementation of the following embodiments of the present invention.

The following examples further illustrate the content of the present invention, but should not be construed as limiting the present invention. Without departing from the spirit and essence of the present invention, any modifications or substitutions made to the methods, steps or conditions of the present invention fall within the scope of the present invention.

Embodiment 1: A kind of feed that improves the semen quality of ram

The invention provides a feed for improving the quality of ram semen, which is obtained by mixing a basic ration with L-citrulline, wherein each part of the basic ration uses 400 kg of wheat straw, 220 kg of green grass, 430 kg of alfalfa, and 100 kg of Dabeinong 702 , 186 300kg of fattening fertilizers, 13kg of baking soda, and 9kg of animal husbandry salt are mixed, and the L-citrulline is added according to 2-6mg/head.

Embodiment 2: A kind of feed and application thereof for improving the quality of ram semen

The invention provides a feed for improving the semen quality of rams, which is obtained by mixing a basal ration with L-citrulline, wherein the basal ration adopts 400kg of wheat straw, 220kg of green grass, 430kg of alfalfa, 100kg of Dabeinong 702, 300 kg of fertilizer 186, 13 kg of baking soda, and 9 kg of animal husbandry salt were mixed, and the L-citrulline was added at 2 mg per bird; the dry matter content of the basal ration was 1.26%, and the moisture content of the basal ration was 41%. The invention provides an application of a feed for improving the semen quality of rams in feeding for improving the semen quality of rams. The feeding amount is 0.69 kg per ram, and the feeding frequency is twice a day.

Embodiment 3: A kind of feed and application thereof for improving the quality of ram semen

The invention provides a feed for improving the semen quality of rams, which is obtained by mixing a basal ration with L-citrulline, wherein the basal ration adopts 400kg of wheat straw, 220kg of green grass, 430kg of alfalfa, 100kg of Dabeinong 702, 300 kg of fertilizer 186, 13 kg of baking soda, and 9 kg of animal husbandry salt were mixed, and the L-citrulline was added at 4 mg per bird; the dry matter content of the basal ration was 1.26%, and the moisture content of the basal ration was 41%. The invention provides an application of a feed for improving the semen quality of rams in feeding for improving the semen quality of rams. The feeding amount is 0.69 kg per ram, and the feeding frequency is twice a day.

Embodiment 4: A kind of feed and application thereof for improving the semen quality of rams

The invention provides a feed for improving the semen quality of rams, which is obtained by mixing a basal ration with L-citrulline, wherein the basal ration adopts 400kg of wheat straw, 220kg of green grass, 430kg of alfalfa, 100kg of Dabeinong 702, 300 kg of fertilizer 186, 13 kg of baking soda, and 9 kg of animal husbandry salt were mixed, and the L-citrulline was added at 6 mg per bird; the dry matter content of the basal ration was 1.26%, and the moisture content of the basal ration was 41%. The invention provides an application of a feed for improving the semen quality of rams in feeding for improving the semen quality of rams. The feeding amount is 0.69 kg per ram, and the feeding frequency is twice a day.

Embodiment 5: A kind of feed that improves the semen quality of ram and its application effect

Based on the technical solutions provided in the above-mentioned Examples 2 to 4, 32 1-year-old healthy Turpan black sheep rams were selected for training and semen collection, with an average body weight of (62.65±5.79) kg, and a control group and test groups I and II were set up. , Group Ⅲ, the experimental group and the control group were fed with the same feeding management conditions except that they were fed with different feeds. During the feeding process, blood samples and semen samples were collected from the rams in the pen on days 0, 15, 30, 45, and 60, and the content of reproductive hormones, antioxidant substances, and semen in the relevant serum and semen were determined. Quality is tested.

1. Effects of different doses of L-citrulline supplementation on the content of reproductive hormones and antioxidant substances in serum and semen of rams

The measurement results were analyzed using SAS software, and the analysis results are shown in Table 1, Table 2, Table 3, Table 4, and Table 5 below.

Table 1: Effects of different doses of L-citrulline supplementation on serum reproductive hormone levels in rams

Note: different lowercase letters on the shoulders of peer data indicate significant differences (P<0.05), the same lowercase letters or no letters on shoulders indicate no significant differences (P>0.05); different uppercase letters on peer data shoulders indicate extremely significant differences (P <0.01), the same below.

Table 2: Effects of different doses of L-citrulline supplementation on antioxidant capacity of ram serum

Table 3: Effects of different doses of L-citrulline supplementation on the level of reproductive hormones in ram semen

Table 4: Effects of different doses of L-citrulline supplementation on antioxidant capacity of ram semen

As shown in the above table 1, table 2, table 3, and table 4 analysis results, through the determination of the technical level and antioxidant capacity substances in the ram serum and semen, there are significant differences in each index between each test group and the control group. The difference shows that the technical solution provided by the present invention has a significant effect on improving the quality of ram semen.

2. Effects of feeding different doses of L-citrulline on the semen quality of rams

The results are shown in Table 5. Compared with the control group and the test groups Ⅰ and Ⅱ, the amount of ejaculate in group III increased by 4.67%, 4.67% and 7.69%, respectively, but the difference among the groups was not significant. Compared with the control group, the sperm density test groups Ⅰ, Ⅱ, and Ⅲ increased by 5.89% (P<0.01), 7.10% (P<0.01) and 18.26% (P<0.01), respectively. Compared with group Ⅱ, they increased by 11.69% (P<0.01) and 1042% (P<0.01), respectively, and there was no significant difference between group Ⅰ and group Ⅱ. Compared with the control group and the test Ⅰ and Ⅱ groups, the dead sperm count test group Ⅲ increased by 10.08% (P<0.01), 8.52% (P<0.01) and 9.47% (P<0.01). There was no significant difference compared with group Ⅱ. The sperm mortality rate in the control group was increased by 4.56% (P<0.05), 6.96% (P<0.01) and 8.43% (P<0.01) compared with the test groups Ⅰ, Ⅱ and Ⅲ, and there was no significant difference between the test groups . There was no significant difference between the control group and the test groups in the number of abnormal sperm and the abnormal rate. Compared with the control group and the test Ⅰ and Ⅱ groups, the number of spermatozoa in the linear movement test group Ⅲ increased by 21.04% (P<0.01), 12.86% (P<0.01) and 10.66% (P<0.01), respectively. Compared with the control group, the experimental group increased by 7.257.10% (P<0.01) and 9.38% (P<0.01), respectively, and there was no significant difference between the experimental group I and the experimental group II. Compared with the control group, the sperm motility test groups Ⅰ, Ⅱ, and Ⅲ increased by 1.56% (P>0.05), 2.29% (P<0.01) and 2.58% (P<0.01) respectively, and there were no differences among the test groups. significantly.

Table 5: Effects of different doses of L-citrulline supplementation on the quality of ram semen (n=8)

It can be seen from the above examples that the feed for improving the quality of ram semen provided in Example 4 of the technical solution provided by the present invention has the best effect on improving the content of reproductive hormones and antioxidant substances in ram serum and semen, It has the best improvement effect on the semen quality index, and the feeding effect is the best, that is, the feed with the addition of 6 mg of L-citrulline combined with the basic diet has the best feeding effect.

Through the cost calculation, the addition of L-citrulline is the main factor affecting the cost of feeding. With the increase of the addition of L-citrulline, the cost will continue. The addition of 6mg/piece of L-citrulline combined with the basic daily After the grain feed has obtained a significantly improved feeding effect, considering the feeding cost and feeding effect comprehensively, the daily cost of each sheep is only 2.65 yuan when the amount of L-citrulline added is 6mg/head, which is the optimal Program.

A feed for improving the semen quality of rams provided by the present invention is used to improve the semen quality of rams by adding basal rations and appropriate proportion of L-citrulline. The improved ram obtained by the technical solution provided by the present invention The cost of semen-quality feed is low, and the daily cost of each sheep is only 2.65 yuan, which can significantly reduce the cost in the breeding process, and has wide practicability in the field of ram breeding.

As mentioned above, the present invention can be better realized. The above-mentioned embodiment is only a description of the preferred implementation of the present invention, and does not limit the scope of the present invention. Various changes and improvements made by technicians to the technical solutions of the present invention shall fall within the scope of protection determined by the present invention.

Claims (4)

1. a feed for improving the quality of ram semen, is characterized in that, adopts basal ration to obtain in conjunction with L-citrulline mixing, wherein said basal ration adopts wheat straw 400kg, green grass 220kg, alfalfa 430kg, Dabei 100 kg of Nong 702, 300 kg of fattening fertilizer 186, 13 kg of baking soda, and 9 kg of animal husbandry salt were mixed, and the L-citrulline was added at 6 mg per bird; the dry matter content of the basal diet was 1.26%. 2. A kind of feed for improving ram semen quality as claimed in claim 1, is characterized in that, described basal ration moisture content is 41%. 3. The application of a feed for improving the quality of ram semen according to any one of claims 1-2 in the preparation of feed for improving the quality of ram semen, characterized in that the feeding amount is 0.69kg/only. 4. the application of a kind of feed improving ram semen quality as claimed in claim 3 in the preparation of improving ram semen quality feed, is characterized in that, feeding times is every day twice.