CN112825862A

CN112825862A - Functional composition suitable for coloring various fruits and method for promoting fruit coloring

Info

Publication number: CN112825862A
Application number: CN202011629115.9A
Authority: CN
Inventors: 郑先福; 万翠
Original assignee: ZHENGZHOU ZHENGSHI CHEMICAL PRODUCT CO Ltd
Current assignee: ZHENGZHOU ZHENGSHI CHEMICAL PRODUCT CO Ltd
Priority date: 2020-12-31
Filing date: 2020-12-31
Publication date: 2021-05-25
Anticipated expiration: 2040-12-31
Also published as: CN112825862B

Abstract

The invention belongs to the field of plant growth regulators, and particularly relates to a functional composition suitable for coloring various fruits and a method for promoting the coloring of the fruits. The active ingredients of the functional composition consist of a first active substance and a second active substance, wherein the first active substance is selected from 2, 4-dichlorobenzoyl amino cyclopropanecarboxylic acid and/or 2, 4-dichlorobenzoyl amino cyclopropanecarboxylic acid salt; the second active substance is at least one selected from choline chloride, propyl dihydrojasmonate and 5-aminolevulinic acid. The functional composition suitable for coloring various fruits, provided by the invention, has a synergistic effect on the aspect of coloring and quality improvement, reduces the effective dosage of the active ingredients by about 90% compared with the prior product, is suitable for different fruit varieties such as tomatoes, apples, winter jujubes, grapes and the like, and has better effectiveness and stability on the aspect of coloring and quality improvement.

Description

Functional composition suitable for coloring various fruits and method for promoting fruit coloring

Technical Field

The invention belongs to the field of plant growth regulators, and particularly relates to a functional composition suitable for coloring various fruits and a method for promoting the coloring of the fruits.

Background

The color of the fruit is an important indicator of the quality of appearance. With the continuous improvement and improvement of living standard of people, the fruit quality is the first requirement of people. The fruit with correct appearance, uniform and beautiful color and strong taste is deeply loved by consumers. Therefore, how to promote the coloring of the fruits and improve the quality of the fruits is a most concerned problem for growers.

Fruit coloring is affected by many factors, such as fruit variety, light, temperature, fruit set, water and fertilizer, endogenous hormone levels, and the like. At present, the research on the application of crop coloring not only strengthens water and fertilizer and illumination management, but also utilizes plant growth regulators to increase the coloring speed, has wide application, and mainly focuses on ethephon, abscisic acid, propyl dihydrojasmonate and other plant growth regulators. However, a large number of field tests and applications find that the phenomenon that the leaves of the grape trees fall, the fruits crack, the fruit grains are threshed, the fruits are not storage-resistant and the like are easily caused by singly applying the ethephon preparation; abscisic acid is sensitive to light, is unstable and easy to decompose in the using process, is expensive and increases the cost. Therefore, there is a need to solve a typical problem occurring in the field of fruit coloring, namely, mild coloring effect, availability for a wide range of crops while having no side effects on quality and even improving crop quality.

Meanwhile, another problem encountered in the market is that the coloring agent with stable effect, such as propyl dihydrojasmonate, can only exert the effect on the varieties which are easy to color and are easy to color under the illumination environment, and has poor performance on the varieties which are difficult to color or are colored under the influence of the illumination environment; also, for example, patent CN 1302697C discloses a method of externally applying 5-aminolevulinic acid (5-ALA) or its precursor, which promotes the fruit coloring mainly by improving the photosensitivity of the peel. The action effect has strong adherence to illumination and is extremely unstable.

In the aspect of fruit coloring function, the applicant studied the regulating agent (CN107410351B) for grapes compounded by 2, 4-dichlorobenzoyl aminocyclopropionic acid and active auxiliary agents in the previous period, and proved that the regulating agent has gain in improving grape coloring and improving grape quality. After long-term follow-up research, the applicant still hopes to further reduce the dosage of the active ingredients, widen the application range of crops and improve the effectiveness and stability of the coloring and quality improving effect.

Disclosure of Invention

The invention aims to provide a functional composition suitable for coloring various fruits, which is suitable for coloring and improving the quality of various fruits, and the effective dose of active ingredients is reduced by about 90 percent when the composition is applied, so that the effectiveness and the stability of the coloring and improving effect are further improved.

A second object of the invention is to provide a method for promoting fruit coloring.

In order to achieve the above object, the present invention provides a functional composition suitable for coloring various fruits, comprising:

a functional composition suitable for the colouring of various fruits, the active principle of which consists of a first active substance selected from 2, 4-dichlorobenzoyl aminocyclopropionic acid and/or 2, 4-dichlorobenzoyl aminocyclopropionic acid salt; the second active substance is at least one selected from choline chloride, propyl dihydrojasmonate and 5-aminolevulinic acid;

when the second active substance contains choline chloride, the weight ratio of the first active substance to the choline chloride is 1: 1-20;

when the second active substance contains propyl dihydrojasmonate, the weight ratio of the first active substance to the propyl dihydrojasmonate is 1: 0.5 to 10;

when the second active substance contains 5-aminolevulinic acid, the weight ratio of the first active substance to the 5-aminolevulinic acid is 1: 0.5 to 5.

The functional composition suitable for coloring various fruits, provided by the invention, has a synergistic effect on the aspect of coloring and quality improvement, reduces the effective dosage of the active ingredients by about 90% compared with the prior product, is suitable for different fruit varieties such as tomatoes, apples, winter jujubes, grapes and the like, and has better effectiveness and stability on the aspect of coloring and quality improvement.

In view of raw material cost, the 2, 4-dichlorobenzoyl amino cyclopropane salt is preferably at least one of sodium salt, potassium salt, ammonium salt and calcium salt of 2, 4-dichlorobenzoyl amino cyclopropane.

Preferably, when used in tomatoes, the weight ratio of the first active substance to the 5-aminolevulinic acid is 1: 0.5 to 1. The weight ratio of the first active substance to the propyl dihydrojasmonate is 1: 0.5 to 1. The weight ratio of the first active substance to the choline chloride is 1:15 to 20. The above proportioning relations can be used alternatively or in combination, the following are the same.

When the active agent is applied to apples, the weight ratio of the first active substance to the 5-aminolevulinic acid is 1:1 to 5. The weight ratio of the first active substance to the propyl dihydrojasmonate is 1:1 to 5. The weight ratio of the first active substance to the choline chloride is 1:1 to 10.

When the composition is used for winter jujubes, the weight ratio of the first active substance to the 5-aminolevulinic acid is 1:1 to 5. The weight ratio of the first active substance to the propyl dihydrojasmonate is 1:5 to 10. The weight ratio of the first active substance to the choline chloride is 1:5 to 10.

When used for grapes, the weight ratio of the first active substance to the 5-aminolevulinic acid is 1:1 to 5. The weight ratio of the first active substance to the propyl dihydrojasmonate is 1:5 to 10. The weight ratio of the first active substance to the choline chloride is 1:1 to 5.

In order to improve the convenience of the composition, it is preferable that the functional composition further comprises a carrier component, and the carrier component is at least one selected from boron fertilizer, potassium fertilizer, amino acid and glucose.

Preferably, when the tomato carrier is used for tomatoes, the carrier component is at least one selected from boric fertilizer, amino acid and glucose. When applied to apple, the carrier component is at least one selected from potassium fertilizer, amino acid and glucose. For winter jujube, the carrier component is selected from amino acids and/or glucose. When used for grape, the carrier component is at least one of boron fertilizer, potassium fertilizer and amino acid.

The boric fertilizer can be boric acid, boric acid ester, potassium borate and the like. The potash fertilizer may be composite potassium phosphate fertilizer, such as potassium dihydrogen phosphate, potassium pyrophosphate, etc., or simple substance potassium fertilizer, such as potassium formate, potassium acetate, potassium sulfate, potassium citrate, etc. The amino acid can be simple substance amino acid such as methionine, phenylalanine, glutamic acid, etc., or compound amino acid raw powder or raw solution. The compound amino acid raw powder or stock solution can be selected from conventional 30% compound amino acid raw powder or stock solution.

Preferably, the mass ratio of the active ingredient to the carrier is 1:1 to 19. More preferably, when the second active material contains 5-aminolevulinic acid, the mass ratio of the active ingredient to the carrier is 1:9 to 16. When the second active substance contains propyl dihydrojasmonate, the mass ratio of the active ingredient to the carrier is 1: 2.5 to 12.5. When the second active substance contains choline chloride, the mass ratio of the active ingredient to the carrier is 1:1 to 4.

When the tomato extract is used for tomatoes, the preferable scheme is as follows: 5% of dichlorobenzoyl amino cyclopropanecarboxylic acid, 2.5% of 5-ALA and 92.5% of compound amino acid; or 5% of sodium dichlorobenzoyl amino cyclopropane, 2.5% of propyl dihydrojasmonate and 92.5% of boric acid ester; or 1% of dichlorobenzoyl amino cyclopropanecarboxylic acid, 20% of choline chloride and 79% of glucose; wherein 5 percent of dichlorobenzoyl aminocyclopropionic acid, 2.5 percent of 5-ALA and 92.5 percent of compound amino acid are optimal.

When the apple juice beverage is used for apples, the preferable scheme is as follows: 1% of dichlorobenzoyl amino cyclopropanecarboxylic acid, 5-ALA and 94% of compound amino acid; or 7.5 percent of calcium dichlorobenzoylamino cyclopropane, 7.5 percent of propyl dihydrojasmonate and 85 percent of potassium citrate; or 10% of dichlorobenzoyl amino cyclopropanecarboxylic acid, 10% of choline chloride and 80% of glucose; wherein, the optimal contents are 7.5 percent of calcium dichlorobenzoylamino cyclopropane, 7.5 percent of propyl dihydrojasmonate and 85 percent of potassium citrate.

When the method is used for winter jujubes, the preferable scheme is as follows: 2.5 percent of dichlorobenzoyl amino cyclopropanecarboxylic acid, 5 percent of 5-ALA and 92.5 percent of compound amino acid; or 2.5% of dichlorobenzoyl aminocyclopropanamide, 5% of propyl dihydrojasmonate and 92.5% of glucose; or 2.5% of dichlorobenzoyl amino cyclopropanecarboxylic acid, 25% of choline chloride and 72.5% of glucose; wherein, 2.5 percent of dichlorobenzoyl amino cyclopropanecarboxylic acid, 25 percent of choline chloride and 72.5 percent of glucose are the best.

When the grape wine is used for grapes, the preferable scheme is as follows: 5% of dichlorobenzoyl amino cyclopropanecarboxylic acid, 5% of 5-ALA and 90% of compound amino acid; or 3% of sodium dichlorobenzoyl amino cyclopropane, 15% of propyl dihydrojasmonate and 82% of boric acid ester; or 10% of potassium dichlorobenzoyl amino cyclopropane, 40% of choline chloride and 50% of potassium dihydrogen phosphate; wherein 5 percent of dichlorobenzoyl aminocyclopropionic acid, 5 percent of 5-ALA and 90 percent of compound amino acid are optimal.

The technical scheme of the method for promoting fruit coloring is as follows:

a method of promoting fruit coloring comprising the steps of: the leaf or fruit is contacted with the functional composition in an effective amount.

The method for promoting the fruit coloring can further reduce the dosage of the active ingredients and effectively maintain the coloring effect, and the input cost of farmers is reduced to a certain extent.

Preferably, the contacting comprises spraying a liquid medicine containing the functional composition to the leaves or the fruits. By taking a medicament consisting of an active ingredient and a carrier as an example, the mass ratio of the first active substance can be controlled to be 1-10%, and the medicament can be directly diluted by 100-1000 times for use according to local climate and water and fertilizer conditions of crops. The method can be used for spraying crop leaves or spraying or dipping clusters.

Particularly, the concentration of the first active substance is as low as 30-100ppm, so that a good effect can be obtained on the grapes; the concentration of the first active substance is as low as 20-200ppm, and good effect on apple can be obtained. The concentration of the first active substance is as low as 20-100ppm, so that a good effect can be obtained on the tomatoes; the concentration of the first active substance is as low as 50-100ppm, and good effect can be obtained on winter jujubes.

Experiments prove that the functional composition has good effects when being used on tomatoes, apples, winter jujubes and grapes.

Drawings

FIG. 1 is a schematic diagram of a salification process of 2, 4-dichlorobenzamide cyclopropanecarboxylic acid in the present invention;

FIG. 2 is a comparison of the coloring effect of different treatments on Fuji and Qiannin apples in accordance with the present invention;

FIG. 3 is a comparison of the coloration of the summer black grapes at 3d and 7d after application on the day of application of the summer black grapes according to the present invention;

FIG. 4 is a comparison of the coloration of the summer black grapes at 3d and 7d after application on the day of application of the summer black grapes in accordance with the second embodiment of the invention;

FIG. 5 is a comparison of the coloring conditions of different treatments of the present invention on the day of administration of the Jingya grape and 3d and 7d after administration;

FIG. 6 is a second comparison of the coloring of the grapes in the present invention at the day of the administration of the grapes in Jingya and 3d and 7d after the administration;

FIG. 7 is a comparison of the 7d coloring effect of different treatments of the present invention on red globe grapes;

FIG. 8 is a comparison of the 14d coloring effect of the honey-crispy concubine grapes treated differently in the present invention;

FIG. 9 is a comparison of the 14d coloration effect of different treatments of the present invention after administration of Krenson grape.

Detailed Description

The following examples are provided to further illustrate the practice of the invention.

In the following experimental examples, the raw materials involved are all common commercial products except for 2, 4-dichlorobenzamide cyclopropanecarboxylic acid and salts thereof.

2. 4-Dichlorobenzamide cyclopropanecarboxylic acid can be prepared by referring to the method in CN 107410351B. The preparation process route of the salt is shown in figure 1.

The alkali salt raw material in the invention is selected from any one of sodium carbonate, potassium carbonate, ethanolamine and calcium hydroxide, wherein the molar ratio of 2, 4-dichlorobenzoyl amino cyclopropanecarboxylic acid to sodium carbonate or potassium carbonate or ethanolamine or calcium hydroxide in the salt forming reaction is 1: 1.

One embodiment of the functional composition suitable for coloring various fruits of the invention

Example 1

The functional composition suitable for coloring various fruits in the embodiment is prepared from the following raw materials in parts by weight for every 100 parts by weight of tomato: 5 parts of dichlorobenzoyl aminocyclopropionic acid, 2.5 parts of 5-ALA and 92.5 parts of compound amino acid. The mass ratio of the active ingredient to the carrier is 1: 12.3.

Weighing the raw materials according to the proportion, adding the raw materials into a mixing kettle, uniformly mixing, and detecting to be qualified to obtain the product.

Example 2

The functional composition suitable for coloring various fruits in the embodiment is prepared from the following raw materials in parts by weight for every 100 parts by weight of tomato: 5 parts of sodium dichlorobenzoyl amino cyclopropane, 2.5 parts of propyl dihydrojasmonate and 92.5 parts of boric acid ester. The mass ratio of the active ingredient to the carrier is 1: 12.3.

Example 3

The functional composition suitable for coloring various fruits in the embodiment is prepared from the following raw materials in parts by weight for every 100 parts by weight of tomato: 1 part of dichlorobenzoyl aminocyclopropionic acid, 20 parts of choline chloride and 79 parts of glucose. The mass ratio of the active ingredient to the carrier is 1: 3.7.

Example 4

The functional composition suitable for coloring various fruits in the embodiment is prepared from the following raw materials in parts by weight for 100 parts by weight of an apple: 1 part of dichlorobenzoyl aminocyclopropionic acid, 5 parts of 5-ALA and 94 parts of compound amino acid. The mass ratio of the active ingredient to the carrier is 1: 15.6.

Example 5

The functional composition suitable for coloring various fruits in the embodiment is prepared from the following raw materials in parts by weight for 100 parts by weight of an apple: 7.5 parts of calcium dichlorobenzoylamino cyclopropanepropionate, 7.5 parts of propyl dihydrojasmonate and 85 parts of potassium citrate. The mass ratio of the active ingredient to the carrier is 1: 5.6.

Example 6

The functional composition suitable for coloring various fruits in the embodiment is prepared from the following raw materials in parts by weight for 100 parts by weight of an apple: 10 parts of dichlorobenzoyl aminocyclopropionic acid, 10 parts of choline chloride and 80 parts of glucose. The mass ratio of the active ingredient to the carrier is 1:4.

Example 7

The functional composition suitable for coloring various fruits in the embodiment is prepared from the following raw materials in parts by weight per 100 parts by weight of the composition for winter jujubes: 2.5 parts of dichlorobenzoyl aminocyclopropionic acid, 5 parts of 5-ALA and 92.5 parts of compound amino acid. The mass ratio of the active ingredient to the carrier is 1: 12.3.

Example 8

The functional composition suitable for coloring various fruits in the embodiment is prepared from the following raw materials in parts by weight per 100 parts by weight of the composition for winter jujubes: 2.5 parts of dichlorobenzoyl aminocyclopropanolamine, 25 parts of dihydro jasmonic acid propyl ester and 72.5 parts of glucose. The mass ratio of the active ingredient to the carrier is 1: 2.6.

Example 9

The functional composition suitable for coloring various fruits in the embodiment is prepared from the following raw materials in parts by weight per 100 parts by weight of the composition for winter jujubes: 5 parts of dichlorobenzoyl aminocyclopropionic acid, 25 parts of choline chloride and 70 parts of glucose. The mass ratio of the active ingredient to the carrier is 1: 2.3.

Example 10

The functional composition suitable for coloring various fruits in the embodiment is prepared from the following raw materials in parts by weight for every 100 parts by weight of grapes: 5 parts of dichlorobenzoyl aminocyclopropionic acid, 5 parts of 5-ALA and 90 parts of compound amino acid. The mass ratio of the active ingredient to the carrier is 1: 9.

Example 11

The functional composition suitable for coloring various fruits in the embodiment is prepared from the following raw materials in parts by weight for every 100 parts by weight of grapes: 3 parts of sodium dichlorobenzoyl amino cyclopropanepropionate, 15 parts of propyl dihydrojasmonate and 82 parts of boric acid ester. The mass ratio of the active ingredient to the carrier is 1: 4.5.

Example 12

The functional composition suitable for coloring various fruits in the embodiment is prepared from the following raw materials in parts by weight for every 100 parts by weight of grapes: 10 parts of potassium dichlorobenzoylaminocyclopropionate, 40 parts of choline chloride and 50 parts of potassium dihydrogen phosphate. The mass ratio of the active ingredient to the carrier is 1: 1.

Second, test example/specific example of the method for promoting fruit coloring of the present invention

Test example 1 coloring, fruit drop and quality of apple

Test time: 9 month 2019

Test site: henan Luoning county Shangg Zhenhai group apple planting base

Test agents: example 4, example 5, example 6 (reported as composition four, composition five, composition six) and control treatment (single dose effective dose selected). The test design of the specific test agents is shown in table 1.

TABLE 1 Fuji apple coloring test design for reagent test

And (3) test crops: fuji apple, Qianyin apple

The application method comprises the following steps: spraying the whole plant 30d before harvest of Fuji apple and Qiannajin apple for 1 time

The investigation method comprises the following steps: investigating the coloring grading of the fruits on the day of applying the pesticide and 7, 14 and 21d after the pesticide, counting the coloring grading of the marked fruits and calculating the coloring index at each investigation, and investigating the fruit drop number and the fruit drop rate; investigating whether the pesticide damage occurs 3 days after the pesticide application; and measuring the quality during collection.

Wherein the color index is graded as the percentage of colored area on each fruit over the entire fruit area.

The classification standard is as follows:

level 0: uncolored;

level 1: the coloring area accounts for less than 20% of the whole fruit area;

and 2, stage: the coloring area accounts for 21 to 40 percent of the whole fruit area;

and 3, level: the coloring area accounts for 41 to 60 percent of the whole fruit area;

4, level: the coloring area accounts for 61-80% of the whole fruit area;

and 5, stage: the coloring area accounts for 81-100% of the whole fruit area.

The evaluation method of the soluble solid content comprises the following steps: the content of total soluble solids in the fruit is measured by using a handheld refractometer, the sugar content of the fruit can be approximately represented, and the higher the content is, the higher the sugar degree is, and the better the quality is.

TABLE 2 results of 21-day color, fruit drop and quality investigation of Fuji apples after application

TABLE 3 investigation results of 21 days after application of Geranium apple for coloration, fruit drop and quality

According to the experimental results of the above tables 2 and 3, the functional compositions of the examples have significant improvement effects in terms of coloring and fruit drop rate reduction, and a synergistic effect can be obtained between the two active substances, compared with the single and control agents.

The coloring effect of blank Control (CK), composition five (treatment B) and control agent 40% ethephon (treatment H) on fuji and qianalin is shown in figure 2.

As can be seen from fig. 1, the application of the functional composition of the embodiment of the present invention can significantly promote the coloring of apples, has a significant coloring effect compared to the use of a single agent, effectively inhibits fruit drop, has a stable effect, promotes the quality of apples to a certain extent, and can increase the solid content by 11.7-12.7%. Has stable effect on different varieties of apples, such as Fuji and Qianyin.

Test example 2 coloring, dehiscent fruit and quality of grape

Test time: year 2020, 4-11

Test site: spring of river, Shanxi fortune city, Yunnan red river state

Test agents: examples 10, 11, 12 (reported as composition ten, composition eleven, composition twelve) and control treatment. The test design of the specific test agents is shown in Table 4.

TABLE 4 test design of test reagent for coloring test of grapes

Processing number	Name of medicament	Dilution factor	Method of administering a drug
				1	Composition ten	1000 times of	Foliage spraying
2	Composition eleven	1000 times of	Foliage spraying
				3	Composition of twelve	1000 times of	Foliage spraying
4	Composition ten	1000 times of	Spray ear
				5	Composition eleven	1000 times of	Spray ear
6	Composition of twelve	1000 times of	Spray ear
				7	40% ethephon	1000 times of	Foliage spraying
8	10% of abscisic acid	500 times of	Spray ear
				CK	Clear water control	0	Spraying

And (3) test crops: grape, including Xiahu, hong Ting, Keleng, Jingya, honey crisp Xiangfei, etc

The application method comprises the following steps: spraying or treating for 1 time on leaf surface at initial color change stage

The investigation method comprises the following steps: investigating the coloring grading of the fruits on the day of pesticide application and 3, 7 and 14d after pesticide application, counting the coloring grading of the marked fruits and calculating the coloring index at each investigation, and investigating the fruit drop number and the fruit drop rate; investigating whether the pesticide damage occurs 3 days after the pesticide application; and (5) detecting the content of soluble solids of the fruit grains 14d after the application.

Wherein, the grape fruit coloring grading is evaluated according to the following standard:

level 0: completely green;

level 1: coloring a little, wherein pink fruit grains account for 1% -10% of the whole ear;

and 2, stage: the colored fruit grains (pink) account for 10-50% of the fruit cluster;

and 3, level: the colored fruit grains account for 50-100% of the whole spike;

4, level: all are colored. Part of black fruit grains appear, and the black fruit grains account for 1-50% of the whole ear;

and 5, stage: the total colored brown fruit grains are more and account for 50-100% of the total spike.

The method of evaluating the coloring index and the soluble solid content was the same as in test example 1.

(1) Summer black grape

The coloration, fruit cracking rate and quality of the summer black grapes after application are shown in Table 5.

TABLE 5 coloring, fruit cracking and quality of summer black grapes after application

The coloring of the Xiahai grape on the day of administration and after 3d and 7d is shown in fig. 3 and 4. Wherein, fig. 3 is the comparative effect of foliage spraying, and fig. 4 is the comparative effect of the spike spraying. The results show that the spraying of the ears or leaves can achieve a considerable coloring effect under the same concentration and different application conditions.

(2) Jingya grape

The coloring, fruit cracking rate and quality of the Jingya grape after application are shown in Table 6

TABLE 6 investigation results of coloring, cracking rate and quality of the Jingya grapes after application

The coloring of the grapes in Jingya on the day of administration and after 3d and 7d is shown in FIG. 5 and FIG. 6. Wherein, fig. 5 is the comparative effect of foliage spraying, and fig. 6 is the comparative effect of spike spraying.

The data of the summer black grape and the Beijing ya grape are combined, so that the scheme of the invention can effectively promote the coloring of the summer black grape and the Beijing ya grape, the coloring is promoted after 3 days, and the coloring is promoted to nearly 100% after 7 days. Meanwhile, compared with a contrast medicament, the fruit powder is not damaged, the fruit does not fall, and the fruit quality is obviously improved. The composition is preferably ten, and can stably exert the coloring and quality improving effects in the aspects of foliage spraying and spike spraying treatment. To verify the stability of its effect on other grape varieties, the authors verified the wide range of applicability of the grape varieties red grape, krusen, honey-crisp princess, etc. (example 10).

(3) Red globe grape

The coloring effect of the red globe grape after the drug application for 7 days is shown in FIG. 7.

(4) Honey crisp fragrant imperial concubine grape

The coloring effect of the honey-crisp Xiangfei grape after the administration for 14d is shown in figure 8.

According to the scheme of the invention, the leaf spraying or spike spraying treatment on the red globe grapes and the honey-crisp Xiangfei grapes can promote uniform coloring, and the fruit powder is not influenced; the contrast agent ethephon has certain negative effects on the color uniformity and the abscisic acid has certain negative effects on the aspect of fruit powder.

(5) Kren grape

The 14d coloring effect of the krusei grape after drug administration is shown in fig. 9.

According to the scheme of the invention, the leaf spraying or ear spraying treatment on the Kron grapes can promote the fruit coloring to different degrees, while the control medicament resistance treatment has little effect on the Kron variety.

Experimental example 3 coloring of tomato

Test time: 6-7 months in 2020

Test site: mu in river south

Test agents: examples 1, 2, 3 (recorded as composition one, composition two, composition three) and control treatment. The test design of the specific test agents is shown in Table 7.

TABLE 7 tomato coloring test design for reagent

Processing number	Name of medicament	Dilution factor	Method of administering a drug
				1	Composition I	1000 times of	Foliage spraying
2	Composition II	500 times of	Foliage spraying
				3	Composition III	500 times of	Foliage spraying
4	40% ethephon	800 times of	Foliage spraying
				CK	Clear water control	0	Spraying

And (3) test crops: tomato

The application method comprises the following steps: spraying and treating the leaf surfaces of the tomato fruits for 1 time when the tomato fruits turn from green to white

The investigation method comprises the following steps: the coloration of the fruits was investigated 7d after application and the appearance, shape and hardness of the fruits and the number of days of early ripening of the fruits relative to the blank were recorded. And after the fruits are completely ripe, selecting 6 fruits in each cell, carrying out sample preparation and measuring the Vc content in the fruits by adopting a titration method.

The coloring and quality of the tomatoes after application are shown in Table 8.

TABLE 8 coloring and quality of tomatoes after application

The tomato is sprayed and treated on the tomatoes in the scheme of the invention, so that the coloring of the tomatoes can be promoted to different degrees, the coloring effect of the contrast medicament ethephon can be achieved, and the tomato can appear on the market 5-6 days earlier than a blank treatment area. Has promoting effect on fruit hardness and Vc content. Coloring, while ethephon treatment as a control agent causes softening of fruits and deterioration of fruit quality.

Experimental example 4 coloring condition of winter jujube

Test time: 8-10 months in 2020

Test site: shanxi Lin 29463

Test agents: examples 7, 8, 9 (recorded as composition seven, composition eight, composition nine) and control treatment. The test design of the specific test agents is shown in Table 7.

TABLE 9 test design of test reagent for tomato coloring test

Processing number	Name of medicament	Dilution factor	Method of administering a drug
				1	Composition seven	500 times of	Foliage spraying
2	Composition eight	500 times of	Foliage spraying
				3	Composition nine	500 times of	Foliage spraying
4	40% ethephon	400 times of	Foliage spraying
				CK	Clear water control	0	Spraying

And (3) test crops: winter jujube

The application method comprises the following steps: spraying the whole plant for 1 time when the fruit turns from green to white

The investigation method comprises the following steps: investigating the coloring grade of the fruits 21d after the pesticide is applied, counting the coloring grade of the marked fruits and calculating the coloring index in each investigation, and simultaneously investigating the fruit drop number and the fruit drop rate; and measuring the quality during collection. The measurement method is referred to as test 1.

The coloring and quality of winter jujubes after application are shown in Table 10.

TABLE 10 investigation results of coloring, fruit dropping and quality of winter jujube 21 days after application

The spraying treatment on the winter jujubes can promote the winter jujubes to be colored to different degrees, can achieve the coloring effect of the ethephon serving as a contrast agent, and can avoid the negative effect of a large amount of fruit drop caused by ethephon serving as the winter jujubes. And the treatment of each composition has the effect of promoting the quality of winter jujubes.

Claims

1. Functional composition suitable for the colouring of various fruits, characterized in that the active principle of the functional composition consists of a first active substance selected from 2, 4-dichlorobenzamido cyclopropanecarboxylic acid and/or 2, 4-dichlorobenzamido cyclopropanecarboxylate; the second active substance is at least one selected from choline chloride, propyl dihydrojasmonate and 5-aminolevulinic acid;

2. The functional composition suitable for coloring fruit according to claim 1, wherein said salt of 2, 4-dichlorobenzoyl aminocyclopropionic acid is at least one of sodium salt, potassium salt, ammonium salt and calcium salt of 2, 4-dichlorobenzoyl aminocyclopropionic acid.

3. Functional composition according to claim 1, suitable for the coloring of various fruits, characterized in that the weight ratio of the first active substance to the 5-aminolevulinic acid, for tomato applications, is 1: 0.5 to 1; the weight ratio of the first active substance to the propyl dihydrojasmonate is 1: 0.5 to 1; the weight ratio of the first active substance to the choline chloride is 1: 15-20;

when the active agent is applied to apples, the weight ratio of the first active substance to the 5-aminolevulinic acid is 1: 1-5; the weight ratio of the first active substance to the propyl dihydrojasmonate is 1: 1-5; the weight ratio of the first active substance to the choline chloride is 1: 1-10;

when the composition is used for winter jujubes, the weight ratio of the first active substance to the 5-aminolevulinic acid is 1: 1-5; the weight ratio of the first active substance to the propyl dihydrojasmonate is 1: 5-10; the weight ratio of the first active substance to the choline chloride is 1: 5-10;

when used for grapes, the weight ratio of the first active substance to the 5-aminolevulinic acid is 1: 1-5; the weight ratio of the first active substance to the propyl dihydrojasmonate is 1: 5-10; the weight ratio of the first active substance to the choline chloride is 1:1 to 5.

4. Functional composition suitable for the coloring of various fruits according to any one of claims 1 to 3, characterized in that it further comprises a carrier ingredient selected from at least one of boric fertilizer, potash fertilizer, amino acids, glucose.

5. The functional composition for coloring fruits according to claim 4, wherein the mass ratio of the active ingredient to the carrier is 1:1 to 19.

6. A method of promoting fruit coloring, comprising the steps of: contacting the leaf or fruit with the functional composition of any one of claims 1-5 in an effective amount.

7. The method for promoting fruit coloring as claimed in claim 6, wherein said contacting comprises spraying a liquid medicine containing said functional composition to the leaves or the fruits.

8. A method according to claim 6 or claim 7, wherein the fruit is tomato, apple, winter jujube or grape.