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CN112805371A - 基于CRISPR/Cas系统对细胞进行基因编辑的方法 - Google Patents

基于CRISPR/Cas系统对细胞进行基因编辑的方法 Download PDF

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Publication number
CN112805371A
CN112805371A CN201980058264.3A CN201980058264A CN112805371A CN 112805371 A CN112805371 A CN 112805371A CN 201980058264 A CN201980058264 A CN 201980058264A CN 112805371 A CN112805371 A CN 112805371A
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cells
cell
trac
gene
antigen
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李宗海
廖朝晖
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Clegg Medical Co ltd
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Cafa Therapeutics Ltd
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Abstract

提供了一种基于CRISPRlCas系统对细胞进行基因编辑的方法，其中Cas酶为酶活力为0‑1nmoI的Cas9酶。还提供了一种构建通用型T细胞的方法，制备得到的T细胞及其用途，其中通过基因编辑技术对T细胞的TCR基因和MHC基因进行基因编辑。还提供了一种gRNA构建体。

Description

PCT国内申请，说明书已公开。

Claims (30)

  1. PCT国内申请，权利要求书已公开。
CN201980058264.3A 2018-09-21 2019-09-23 基于CRISPR/Cas系统对细胞进行基因编辑的方法 Pending CN112805371A (zh)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
CN201811117875 2018-09-21
CN2018111178754 2018-09-21
CN2018111408703 2018-09-28
CN201811140870 2018-09-28
CN201910809475 2019-08-29
CN201910809598 2019-08-29
CN2019108095981 2019-08-29
CN2019108094758 2019-08-29
PCT/CN2019/107374 WO2020057668A1 (zh) 2018-09-21 2019-09-23 基于CRISPR/Cas系统对细胞进行基因编辑的方法

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CN201980058264.3A Pending CN112805371A (zh) 2018-09-21 2019-09-23 基于CRISPR/Cas系统对细胞进行基因编辑的方法
CN201980061824.0A Active CN112739817B (zh) 2018-09-21 2019-09-23 表达有嵌合受体的t细胞

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US (2) US20220016166A1 (zh)
EP (1) EP3854871A4 (zh)
JP (1) JP2022511345A (zh)
KR (1) KR20210065141A (zh)
CN (2) CN112805371A (zh)
AU (1) AU2019341365A1 (zh)
CA (1) CA3122131A1 (zh)
WO (2) WO2020057668A1 (zh)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113827731A (zh) * 2021-09-22 2021-12-24 北京远胜达生物科技发展有限公司 Crispr基因编辑联合pd-1抗体在治疗癌症中的应用
WO2023066243A1 (zh) * 2021-10-18 2023-04-27 西安宇繁生物科技有限责任公司 一种将目的基因定点整合至免疫细胞特定位点的方法及其应用
WO2023193800A1 (zh) * 2022-04-07 2023-10-12 恺兴生命科技(上海)有限公司 嵌合多肽及其应用
WO2023202116A1 (zh) * 2022-04-20 2023-10-26 山东舜丰生物科技有限公司 Cas酶和系统以及应用

Families Citing this family (9)

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