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CN112739829A - 测序文库的构建方法和得到的测序文库及测序方法 - Google Patents

测序文库的构建方法和得到的测序文库及测序方法 Download PDF

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CN112739829A
CN112739829A CN201880097792.5A CN201880097792A CN112739829A CN 112739829 A CN112739829 A CN 112739829A CN 201880097792 A CN201880097792 A CN 201880097792A CN 112739829 A CN112739829 A CN 112739829A
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sequence
nucleic acid
sequencing
acid molecules
fragment
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崔路漫
范飞
章文蔚
龙舟
汪为茂
王欧
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BGI Shenzhen Co Ltd
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Abstract

一种测序文库的构建方法和得到的测序文库及测序方法，构建方法包括：将线性核酸分子环化形成环状核酸分子，滚环扩增得到多拷贝的长片段核酸分子，然后合成互补链得到双链的长片段核酸分子；与转座复合体混合孵育形成带有转座复合体的长片段核酸分子，与带有分子标签序列的固相载体混合孵育，使得分子标签序列与转座复合体上的转座子序列连接；将转座复合体上的转座酶从长片段核酸分子上释放出来，同时长片段核酸分子被打断成多个连接有转座子序列和分子标签序列的短片段核酸分子；对短片段核酸分子进行聚合酶链式扩增，得到测序文库。

Description

PCT国内申请，说明书已公开。

Claims (19)

  1. PCT国内申请，权利要求书已公开。
CN201880097792.5A 2018-09-27 2018-09-27 测序文库的构建方法和得到的测序文库及测序方法 Pending CN112739829A (zh)

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Cited By (4)

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Publication number Priority date Publication date Assignee Title
CN113667716A (zh) * 2021-08-27 2021-11-19 北京医院 基于滚环扩增的测序文库构建方法及其应用
CN113981548A (zh) * 2021-11-24 2022-01-28 竹石生物科技(苏州)有限公司 Dna甲基化测序文库的制备方法和甲基化检测方法
WO2024138690A1 (zh) * 2022-12-30 2024-07-04 深圳华大生命科学研究院 时空转录组测序方法、构建测序文库方法和基因测序系统
WO2025043544A1 (zh) * 2023-08-30 2025-03-06 深圳华大生命科学研究院 一种免疫组库的测序方法

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CN114807123B (zh) * 2021-01-29 2023-12-01 深圳华大基因科技服务有限公司 一种dna分子的扩增引物设计和连接方法
EP4450634A1 (en) * 2021-12-15 2024-10-23 Nanjing GenScript Biotech Co., Ltd. Method for detecting integration site
WO2023240610A1 (zh) * 2022-06-17 2023-12-21 深圳华大智造科技股份有限公司 单链核酸分子测序文库构建方法

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CN1944676A (zh) * 2006-10-20 2007-04-11 东南大学 全基因组滚环扩增及其产物的固定方法
CN102264914A (zh) * 2008-10-24 2011-11-30 阿霹震中科技公司 用于修饰核酸的转座子末端组合物和方法
WO2012061832A1 (en) * 2010-11-05 2012-05-10 Illumina, Inc. Linking sequence reads using paired code tags
WO2012106546A2 (en) * 2011-02-02 2012-08-09 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
CN108138364A (zh) * 2014-09-12 2018-06-08 深圳华大智造科技有限公司 一种核酸单链环状文库的构建方法和试剂
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CN104711250A (zh) * 2015-01-26 2015-06-17 北京百迈客生物科技有限公司 一种长片段核酸文库的构建方法
WO2016191618A1 (en) * 2015-05-27 2016-12-01 Jianbiao Zheng Methods of inserting molecular barcodes
WO2017151828A1 (en) * 2016-03-01 2017-09-08 Universal Sequencing Technology Corporation Methods and kits for tracking nucleic acid target origin for nucleic acid sequencing
CN105861710A (zh) * 2016-05-20 2016-08-17 北京科迅生物技术有限公司 测序接头、其制备方法及其在超低频变异检测中的应用

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113667716A (zh) * 2021-08-27 2021-11-19 北京医院 基于滚环扩增的测序文库构建方法及其应用
CN113667716B (zh) * 2021-08-27 2023-12-15 北京医院 基于滚环扩增的测序文库构建方法及其应用
CN113981548A (zh) * 2021-11-24 2022-01-28 竹石生物科技(苏州)有限公司 Dna甲基化测序文库的制备方法和甲基化检测方法
CN113981548B (zh) * 2021-11-24 2023-07-11 竹石生物科技(苏州)有限公司 Dna甲基化测序文库的制备方法和甲基化检测方法
WO2024138690A1 (zh) * 2022-12-30 2024-07-04 深圳华大生命科学研究院 时空转录组测序方法、构建测序文库方法和基因测序系统
WO2025043544A1 (zh) * 2023-08-30 2025-03-06 深圳华大生命科学研究院 一种免疫组库的测序方法

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US20210214783A1 (en) 2021-07-15
EP3859014A4 (en) 2022-04-27
WO2020061903A1 (zh) 2020-04-02
EP3859014A1 (en) 2021-08-04

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