CN112680340B - Full-automatic nucleic acid detection device and detection method thereof - Google Patents
Full-automatic nucleic acid detection device and detection method thereof Download PDFInfo
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- CN112680340B CN112680340B CN202011544931.XA CN202011544931A CN112680340B CN 112680340 B CN112680340 B CN 112680340B CN 202011544931 A CN202011544931 A CN 202011544931A CN 112680340 B CN112680340 B CN 112680340B
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Abstract
The invention provides a full-automatic nucleic acid detection device and a detection method thereof, wherein the full-automatic nucleic acid detection device comprises a motion module and a detection module; the detection module comprises a fixing module and a detection module, wherein a heating piece and a cooling piece are arranged on the fixing module, the detection module comprises a nucleic acid extraction piece and a nucleic acid amplification piece fixed at one end of the nucleic acid extraction piece, the central area of the nucleic acid extraction piece is a nucleic acid extraction area, and a semi-sealing area is marked between the nucleic acid extraction area and the nucleic acid amplification piece; the motion module include drive module, compress tightly module and extrusion module, drive module respectively independent transmission connect fixed module and extrusion module, drive module drive fixed module compress tightly half seal district, drive module drive extrusion module extrude nucleic acid extraction district, mix nucleic acid extraction reagent and the sample that awaits measuring in the nucleic acid extraction district.
Description
Technical Field
The invention belongs to the technical field of nucleic acid detection, and relates to a full-automatic nucleic acid detection device and a detection method thereof.
Background
Traditional nucleic acid detection, sample pretreatment, nucleic acid extraction and nucleic acid detection are required to be completed step by step and independently, each link is required to be operated by combining specific equipment or instruments, the steps are complex, and the operation is required to be performed in a specific professional detection laboratory.
POCT is a new method for analyzing various biological specimens immediately on a sampling site, upgrading and improving complex processing procedures and programs of various biological specimens in laboratory inspection by technology, and on the premise of not influencing the final experimental result, the whole experimental process is simple, and no or few equipment is needed, so that the inspection result is obtained quickly. The committee for the POCT equipment technical profession of the chinese medical equipment association defines it as: a detection mode which is carried out on a sampling site and rapidly obtains a detection result by using a portable analytical instrument and a matched reagent.
POCT is one of the most important research directions in the biomedical field at present. In recent years, POCT with miniaturized experimental instruments, simplified operation and instant reporting results is increasingly favored by people based on the high-speed development of semiconductor technology and the rapid progress of medical science, high-efficiency and fast-paced working modes and the practical requirements of experimental sites. Currently, the main POCT products in the market at home and abroad are basically developed based on immunology by using a colloidal gold technology or a chemiluminescence technology, and POCT products aiming at nucleic acid detection are research hotspots in the global scope, and only very few nationwide in-vitro diagnostic reagents with core technology are very popular at home and abroad.
CN111704993a discloses an integrated molecular nucleic acid POCT device comprising a reagent cartridge and a cartridge holder; different cavities connected with each other are arranged in the reagent card box, and each cavity is correspondingly provided with a valve switch for controlling the on-off of liquid. The cartridge support provides functions of on-off of a switch, movement of a piston, heating and temperature measurement, judging whether the cartridge exists or not and the like for the reagent cartridge, and plays a role in supporting and positioning. The integrated device allows the direct addition of samples such as nasopharyngeal swab, genital tract swab, sputum, blood and the like without other treatment, thereby realizing the integrated detection of 'sample in and result out'.
CN107964505A discloses a multifunctional nucleic acid detecting device, which comprises a base, a transmission mechanism, a liquid absorbing mechanism, a suction mechanism and a shell sealed with the base. By arranging a plurality of liquid tanks on the base, multiple functions can be integrated, such as extraction, amplification and detection of nucleic acid, or amplification and detection of at least one extracted heavy target template, etc. The multifunctional nucleic acid detection device is in sealing fit with the shell through the base to form an independent cavity, and the liquid suction mechanism can be switched among different liquid tanks through the transmission mechanism, the liquid suction mechanism and the suction mechanism carried by the operating device, and liquid suction or liquid discharge in different liquid tanks can be realized.
CN111704993a discloses an integrated molecular nucleic acid POCT device and method, which consists of a reagent cartridge and a cartridge holder. Different cavities connected with each other are arranged in the reagent card box, and each cavity is correspondingly provided with a valve switch for controlling the on-off of liquid. The cartridge support provides functions of on-off of a switch, movement of a piston, heating and temperature measurement, judging whether the cartridge exists or not and the like for the reagent cartridge, and plays a role in supporting and positioning. The integrated device allows the direct addition of samples such as nasopharyngeal swab, genital tract swab, sputum, blood and the like without other treatment, thereby realizing the integrated detection of 'sample in and result out'.
However, in general, sample pretreatment is complex and lengthy, the amplification and detection processes are also time-consuming, the detection processes are also precise, large, complex, precise and expensive instruments are required, biological cross contamination and biological safety problems are easy to generate, and the requirements on personnel, environment and instruments are relatively high, so that the method is not suitable for rapid diagnosis beside a basic bed, on site and in the field.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a full-automatic nucleic acid detection device and a detection method thereof, and the integration and automation of the extraction, amplification and detection processes of nucleic acid are realized through the cooperation of a detection module and a motion module, so that the whole detection process does not need excessive manual participation, the automation of the nucleic acid detection process is realized, the professional requirements on operators are low, and the application scene of the device is greatly expanded.
To achieve the purpose, the invention adopts the following technical scheme:
In a first aspect, the present invention provides a fully automated nucleic acid detection apparatus comprising a motion module and a detection module.
The detection module comprises a fixing module and a detection module, and the fixing module is used for fixing the detection module; the fixed module on be provided with heating member and cooling member, heating member and cooling member be used for heating and cooling to detecting the module.
The detection module comprises a nucleic acid extraction part and a nucleic acid amplification part fixed at one end of the nucleic acid extraction part, wherein the central area of the nucleic acid extraction part is a nucleic acid extraction area, a sample to be detected is injected into the nucleic acid extraction area before detection starts, and a semi-sealing area is marked between the nucleic acid extraction area and the nucleic acid amplification part.
The motion module include drive module, compress tightly module and extrusion module, drive module respectively independent transmission connect fixed module and extrusion module, drive module drive fixed module compress tightly half seal district, drive module drive extrusion module extrude nucleic acid extraction district, mix nucleic acid extraction reagent and the sample that awaits measuring in the nucleic acid extraction district.
According to the invention, the extraction process and the amplification process of the nucleic acid are integrated into the detection module, the heating function and the cooling function are integrated into the fixing module, and meanwhile, the fixing module also plays a role of fixing the extraction detection module, so that the integral structure layout between the fixing reaction module and the extraction detection module is realized. The nucleic acid detection device has the advantages that the movement module is additionally arranged, the mixing of the nucleic acid extraction reagent injected into the detection module and the sample to be detected is realized through a reciprocating extrusion mode, the integration and automation of the extraction, amplification and detection processes of nucleic acid are realized through the cooperation of the detection module and the movement module, the molecular diagnosis flow is simplified, the technical threshold of the integrated molecular diagnosis is reduced, excessive manual participation is not needed in the whole detection process, an operator only needs to add the sample into a nucleic acid extraction area, the subsequent process is automatically completed by the nucleic acid detection device provided by the invention, the automation of the nucleic acid detection process is realized, the professional requirements on the operator are low, and the application scene of the operator is greatly expanded.
As a preferable technical scheme of the invention, the nucleic acid extraction piece comprises a first fixing frame, a detection bag and a second fixing frame, wherein the first fixing frame and the second fixing frame are buckled relatively and then are used for fixing the detection bag.
Preferably, one end of the detection packet is fixed with the nucleic acid amplification piece, and the other end of the detection packet is fixed with a plastic nozzle.
Preferably, one end of the detection package is fixed with the plastic nozzle in a heat sealing way, and the other end of the detection package is fixed with the nucleic acid amplification piece in a heat sealing way.
Preferably, a plastic mouth cover is arranged at the mouth of the plastic mouth and is used for sealing the mouth of the plastic mouth.
Preferably, the plastic mouth cover is fixed at the mouth of the plastic mouth by direct plug-in or screw-in.
Preferably, the central area of the detection package is a nucleic acid extraction area, a compaction area is marked between the nucleic acid extraction area and the plastic mouth, and a semi-sealing area is marked between the nucleic acid extraction area and the nucleic acid amplification piece.
Preferably, the nucleic acid extraction zone is divided into at least one extraction zone.
The invention divides the nucleic acid extraction area into a plurality of extraction areas, and different extraction reagents or filter materials are injected into different extraction areas in advance, so that the integration of different reactions in space is realized, the reaction period is shorter, the extraction detection efficiency is high, the automation of the nucleic acid detection process is easy to realize, the manual intervention and environmental exposure in the reaction process are also reduced, the tightness of the nucleic acid extraction area is ensured, and the situations of nucleic acid pollution or aerosol diffusion and the like in the reaction process are avoided.
Preferably, at least one filtering area is also arranged between the nucleic acid extraction area and the nucleic acid amplification piece, and filtering materials are filled in the filtering area.
In the present invention, the reagent mixture is purified by adding a filter material, mixing the reagent mixture with the filter material while the reagent mixture flows through the extraction partition containing the filter material, and adsorbing impurities in the reagent mixture by the filter material.
Preferably, the nucleic acid amplification part comprises an amplification cabin and a connection cabin communicated with the amplification cabin, one end of the detection bag wraps the connection cabin, and after the detection bag and the connection cabin are heat-sealed, the connection cabin and the detection bag are fixedly connected.
Preferably, the outer surface of one side of the connecting cabin, which is contacted with the detection bag, is provided with a convex pattern.
Preferably, a filter element is arranged in the connecting cabin, and the reagent mixture obtained by the reaction in the detection bag enters the amplification cabin after being filtered by the filter element.
In the invention, the filter element has two main functions, namely, when the amplification detection area and the nucleic acid extraction area are in recanalization, the dry powder of the amplification system in the amplification detection area is transferred to the detection package due to the negative pressure generated by the recanalization, so that the amplification reaction can not be detected; and secondly, in order to filter the nucleic acid sample extracted by the nucleic acid extraction region when entering the amplification detection region, the purification effect of the nucleic acid of the pretreatment sample is achieved.
Preferably, an amplification detection area of a transparent shell is arranged in the amplification cabin, and a semi-sealing area is marked between the amplification detection area and the adjacent extraction area.
Preferably, the amplification detection area is divided into at least one detection area which is mutually independent, and freeze-dried powder is filled in the detection area.
As a preferable technical scheme of the invention, the fixing module comprises a fixing plate and a reaction plate which are buckled relatively, and the detection module is fixed between the fixing plate and the reaction plate.
Preferably, a guiding cavity is formed between the fixing plate and the reaction plate, and the detection module is inserted into the guiding cavity.
Preferably, a side surface of the fixing plate, which is close to the reaction plate, is marked as a joint surface, a U-shaped protruding guide rail is arranged along three side edges of the joint surface of the fixing plate, and after the fixing plate and the reaction plate are relatively jointed, the protruding guide rail is contacted with the reaction plate, so that a guide cavity is formed between the fixing plate and the reaction plate.
Preferably, in the detection process, the fixed module is vertically placed, and the detection module is vertically inserted into the guide cavity from the top of the fixed module.
Preferably, the bottom of the guiding cavity is provided with a sensing piece, and the sensing module is completely inserted into the guiding cavity and then touches the sensing piece to sense and confirm that the sensing module is successfully inserted.
As a preferable technical scheme of the invention, a rectangular through groove is formed in the central area of the fixing plate, and the nucleic acid extraction area is exposed through the rectangular through groove after the detection module is inserted into the guide cavity.
Preferably, the lens and the fixing block are respectively embedded into the two side surfaces of the fixing plate, and the positions of the lens and the fixing block correspond to each other.
Preferably, a side surface of the fixing plate, which is close to the reaction plate, is marked as a bonding surface, a side surface of the fixing plate, which is far away from the reaction plate, is marked as a deviating surface, the fixing block is embedded into the bonding surface of the fixing plate, the fixing pressing block is used for pressing the nucleic acid amplification piece, and the lens is embedded into the deviating surface of the fixing plate.
Preferably, the lens is a biconvex lens.
As a preferable technical scheme of the invention, the two side surfaces of the reaction plate are respectively embedded with a heating piece and a cooling piece, the heating piece is used for heating the reagent mixture in the extracting piece and the nucleic acid amplification piece, and the cooling piece is used for cooling the reagent mixture in the extracting piece and the nucleic acid amplification piece.
Preferably, the positions of the heating element and the cooling element correspond.
Preferably, a side surface of the reaction plate, which is close to the fixing plate, is marked as a bonding surface, a side surface of the reaction plate, which is far away from the fixing plate, is marked as a deviating surface, the heating element is embedded into the bonding surface of the reaction plate, and the cooling element is embedded into the deviating surface of the reaction plate.
Preferably, the heating element comprises a reaction heating plate and an amplification heating plate which are embedded in the joint surface of the reaction plate side by side, the detection module is inserted into the fixing module, the reaction heating plate corresponds to the position of the nucleic acid extracting element, the amplification heating plate corresponds to the position of the nucleic acid amplifying element, the reaction heating plate is used for heating the reagent mixture in the nucleic acid extracting area, and the amplification heating plate is used for heating or refrigerating the reagent mixture in the amplification detection area.
Preferably, the cooling member comprises a first fan and a second fan which are embedded in the side-by-side relation to the facing surface of the reaction plate.
Preferably, after the detection module is inserted into the fixing module, the first fan corresponds to the position of the nucleic acid extracting part, the second fan corresponds to the position of the nucleic acid amplifying part, the first fan is used for cooling the reagent mixture in the nucleic acid extracting area, and the second fan is used for cooling the reagent mixture in the amplifying detection area.
As a preferable technical scheme of the invention, the motion module comprises a driving module, at least two compression modules and at least one extrusion module, wherein the compression modules and the extrusion modules are transversely arranged side by side in the vertical direction, the compression modules are respectively and correspondingly arranged in a compression area and a semi-sealing area, at least one extrusion module is arranged between two adjacent compression modules, and the extrusion modules correspond to the extraction area; before the detection starts, the driving module drives each compressing module to fixedly compress the compressing area and the semi-sealing area, so that each extracting area is relatively sealed; and then, the driving module drives each extrusion module to reciprocate in the horizontal direction, and the corresponding extraction partition is repeatedly extruded.
Preferably, the extraction partition corresponds to two extrusion modules, the directions of the reciprocating motions of the two extrusion modules corresponding to the same extraction partition are opposite, one extrusion module is horizontally retracted and separated from the extraction partition, and meanwhile, the other extrusion module adjacent to the extraction partition pushes out and extrudes the extraction partition, so that the nucleic acid extraction reagent and the sample to be detected in the extraction partition are fully mixed and extraction reaction is completed to obtain a reagent mixture, after the reaction in the extraction partition is finished, the driving module drives the corresponding compression module to separate from the semi-sealing area, and the driving module drives the corresponding extrusion module to extrude the corresponding extraction partition, so that the extraction partition is communicated with the next adjacent extraction partition, and the reagent mixture flows into the next extraction partition.
As a preferred technical scheme of the invention, the driving module comprises at least three groups of driving components which are transversely arranged side by side in the vertical direction, and each group of driving components is respectively and independently connected with the pressing module or the extruding module in a transmission way.
Preferably, the driving assembly comprises a motor, a gear, a screw rod and a motor guide shaft which are sequentially connected in a transmission way, and the motor guide shaft is connected with the compression module or the extrusion module.
As a preferable technical scheme of the invention, the compression module comprises a compression push plate guide block, a compression push plate connecting block and a consumable tabletting which are sequentially connected, wherein the compression push plate guide block is in transmission connection with the motor guide shaft, and the consumable tabletting is in direct contact with the compression region or the semi-sealing region.
Preferably, the compressing push plate guide block is connected with the compressing push plate connecting block through a snap spring shaft, a spring is sleeved on the periphery of the snap spring shaft, and a snap spring is arranged at the connecting end of the snap spring shaft and the compressing push plate guide block.
Preferably, the extrusion module include extrusion push pedal guide block, extrusion push pedal connection piece and the consumptive material push pedal that connects gradually, extrusion push pedal guide block be connected with motor guiding axle transmission, the consumptive material push pedal with draw subregion direct contact.
Preferably, the extrusion push plate guide block is connected with the extrusion push plate connecting block through a clamp spring shaft, a spring is sleeved on the periphery of the clamp spring shaft, and a clamp spring is arranged at the connecting end of the clamp spring shaft and the extrusion push plate guide block.
Preferably, the contact surface between the consumable pushing plate and the extraction partition is provided with a heating film, and the heating film heats the extraction partition.
Preferably, the movement module further comprises a module mounting shell with a groove structure, and the compression module and the extrusion module are placed in the module mounting shell.
Preferably, the opening of the die set mounting shell is provided with a die set pressing plate, and the die set pressing plate is used for fixing the compression die set and the extrusion die set in the die set mounting shell.
Preferably, the motion module further comprises a motor mounting shell with a groove structure, and the motor is placed in the motor mounting shell.
Preferably, a motor pressing plate is arranged at the opening of the motor mounting shell, and the motor pressing plate is used for fixing the motor in the motor pressing plate.
In a second aspect, the present invention provides a detection method using the nucleic acid detecting apparatus according to the first aspect, the detection method comprising:
Before the detection starts, injecting a sample to be detected into the nucleic acid extraction area, marking a semi-sealing area between the nucleic acid extraction area and the nucleic acid amplification piece, driving the compression module to compress the semi-sealing area by the driving module, and driving the compression module to compress the nucleic acid extraction area by the driving module to mix the nucleic acid extraction reagent in the nucleic acid extraction area with the sample to be detected;
(II) the heating part heats the nucleic acid extraction area to complete the nucleic acid extraction reaction, and then the cooling part cools the nucleic acid extraction area; after the extraction reaction is finished, the compression module is separated from the semi-sealing area, the nucleic acid extraction area is communicated with the nucleic acid amplification piece, and the reagent mixture in the nucleic acid extraction area enters the nucleic acid amplification piece under the extrusion of the extrusion module;
(III) the heating part heats the nucleic acid amplification part to finish the nucleic acid amplification reaction, and the cooling part cools the nucleic acid amplification part after the amplification reaction is finished.
As a preferable technical scheme of the invention, the detection method specifically comprises the following steps:
(1) Before the detection starts, the detection module is vertically arranged, a sample to be detected is injected into a first extraction partition of the nucleic acid extraction area, and a compaction area is marked between the first extraction partition and the plastic nozzle; the half-seal area is marked between two adjacent extraction partitions and between the nucleic acid amplification part and the adjacent extraction partitions;
(2) The driving module drives each compression module to be fixed to the compression area and the semi-sealing area respectively, so that each extraction area forms a closed space, and detection is started;
(3) The driving module corresponding to the first extraction partition drives the extrusion modules to reciprocate in the horizontal direction, the directions of the reciprocating motions of the two extrusion modules corresponding to the first extraction partition are opposite, and the nucleic acid extraction reagent in the first extraction partition and the sample to be detected are fully mixed under the heating of the reaction heating plate to carry out nucleic acid extraction reaction;
(4) Obtaining a reagent mixture after the nucleic acid extraction reaction carried out in the first extraction partition, driving the corresponding compression module to reset by the driving module, separating the compression module from the semi-seal area, and re-communicating the first extraction partition with the adjacent second extraction partition under the extrusion of the compression module, wherein the reagent mixture in the first extraction partition flows into the second extraction partition;
(5) According to the operation logic of the step (4), after the extraction reaction in each extraction partition is finished, the extraction partition is re-communicated with a semi-sealing area between the adjacent next extraction partition, so that reagent mixture in the extraction partition is pushed into the next extraction partition, the reagent mixture flows through each extraction partition one by one and is subjected to nucleic acid extraction reaction in each extraction partition independently, different extraction partitions are controlled to carry out extraction reactions at different temperatures until the last extraction partition is reached, and a first fan and a second fan are started to carry out air cooling on the nucleic acid extraction areas simultaneously;
(6) After the extraction is finished, the semi-sealing area between the last extraction partition and the amplification detection area is communicated again, under the extrusion of the extrusion module, the reagent mixture in the last extraction partition enters the amplification detection area, the amplification detection area is heated by the amplification heating plate, the reagent mixture is mixed with freeze-dried powder, the nucleic acid amplification reaction is completed, and after the amplification reaction is finished, the first fan and the second fan are simultaneously started to cool the amplification detection area, and the extrusion module is reset completely.
Compared with the prior art, the invention has the beneficial effects that:
According to the invention, the extraction process and the amplification process of the nucleic acid are integrated into the detection module, the heating function and the cooling function are integrated into the fixing module, and meanwhile, the fixing module also plays a role of fixing the extraction detection module, so that the integral structure layout between the fixing reaction module and the extraction detection module is realized. The nucleic acid detection device has the advantages that the movement module is additionally arranged, the mixing of the nucleic acid extraction reagent injected into the detection module and the sample to be detected is realized through a reciprocating extrusion mode, the integration and automation of the extraction, amplification and detection processes of nucleic acid are realized through the cooperation of the detection module and the movement module, the molecular diagnosis flow is simplified, the technical threshold of the integrated molecular diagnosis is reduced, excessive manual participation is not needed in the whole detection process, an operator only needs to add the sample into a nucleic acid extraction area, the subsequent process is automatically completed by the nucleic acid detection device provided by the invention, the automation of the nucleic acid detection process is realized, the professional requirements on the operator are low, and the application scene of the operator is greatly expanded.
Drawings
FIG. 1 is a schematic structural diagram of a fixing module according to an embodiment of the present invention;
FIG. 2 is a schematic structural diagram of a detection module according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of a motion module according to an embodiment of the present invention;
FIG. 4 is a schematic view of an extrusion module according to an embodiment of the present invention;
FIG. 5 is a schematic structural diagram of a compression module according to an embodiment of the present invention;
Wherein, 1-fixing plate; 2-reaction plate; 3-a first fan; 4-a second fan; 5-reaction heating plate; 6-a sensing piece; 7-amplifying a heating plate; 8-fixing blocks; 9-a lens; 10-detecting package; 11-a compaction zone; 12-extracting partitions; 13-semi-seal area; 14-plastic mouth; 15-a plastic mouth cover; 16-a filter element; 17-connection pod; 18-amplification compartment; 19-nucleic acid amplification member; 20-a motor pressing plate; 21-an electric motor; 22-a motor mounting case; 23-gear; 24-screw rod; 25-module mounting shells; 26-a motor guide shaft; 27-a compacting module; 28-an extrusion module; 29-a module platen; 30-extruding a push plate guide block; 31-a clamp spring shaft; 32-a spring; 33-snap springs; 34-extruding the push plate connection block; 35-a consumable push plate; 36-compacting the push plate guide block; 37-pressing the push plate connecting block; 38-consumable tabletting.
Detailed Description
It is to be understood that in the description of the present invention, the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus are not to be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first", "a second", etc. may explicitly or implicitly include one or more such feature. In the description of the present invention, unless otherwise indicated, the meaning of "a plurality" is two or more.
It should be noted that, in the description of the present invention, unless explicitly specified and limited otherwise, the terms "disposed," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art in a specific case.
The technical scheme of the invention is further described below by the specific embodiments with reference to the accompanying drawings.
In one embodiment, the invention provides a fully automatic nucleic acid detection device, which comprises a motion module and a detection module.
The detection module comprises a fixing module and a detection module, wherein the fixing module is used for fixing the detection module, a heating piece and a cooling piece are arranged on the fixing module, and the heating piece and the cooling piece are used for heating and cooling the detection module.
As shown in fig. 2, the detection module includes a nucleic acid extraction member and a nucleic acid amplification member 19 fixed at one end of the nucleic acid extraction member, wherein a central area of the nucleic acid extraction member is a nucleic acid extraction region, a sample to be detected is injected into the nucleic acid extraction region before detection starts, and a semi-sealing region 13 is marked between the nucleic acid extraction region and the nucleic acid amplification member 19.
The motion module is shown in fig. 3, and comprises a driving module, a compressing module 27 and an extruding module 28, wherein the driving module is respectively and independently connected with the fixing module and the extruding module 28 in a transmission way, the driving module drives the fixing module to compress the semi-sealing area 13, and the driving module drives the extruding module 28 to extrude the nucleic acid extraction area to mix the nucleic acid extraction reagent in the nucleic acid extraction area with the sample to be detected.
The nucleic acid extraction part comprises a first fixing frame, a detection bag 10 and a second fixing frame, wherein the first fixing frame and the second fixing frame are used for fixing the detection bag 10 after being buckled relatively. As shown in fig. 2, a nucleic acid amplification element 19 is fixed to one end of the detection pack 10, and a plastic nozzle 14 is fixed to the other end of the detection pack 10. One end of the detection pack 10 is heat-sealed and fixed to the plastic nozzle 14, and the other end of the detection pack 10 is heat-sealed and fixed to the nucleic acid amplification element 19. A plastic mouth cover 15 is arranged at the mouth of the plastic mouth 14, and the plastic mouth cover 15 is used for sealing the mouth of the plastic mouth 14. The plastic mouth cover 15 is fixed at the mouth of the plastic mouth 14 by direct plug-in or screw-in. The central area of the detection package 10 is a nucleic acid extraction area, a compaction area 11 is marked between the nucleic acid extraction area and a plastic nozzle 14, a semi-sealing area 13 is marked between the nucleic acid extraction area and a nucleic acid amplification piece 19, the nucleic acid extraction area is divided into at least one extraction partition 12, at least one filtering area is also arranged between the nucleic acid extraction area and the nucleic acid amplification piece 19, and filtering materials are filled in the filtering area.
The nucleic acid amplification part 19 comprises an amplification cabin 18 and a connection cabin 17 communicated with the amplification cabin 18, one end of the detection bag 10 wraps the connection cabin 17, and after the detection bag 10 and the connection cabin 17 are heat-sealed, the connection cabin 17 and the detection bag 10 are fixedly connected. The outer surface of the side of the connection pod 17, which is in contact with the test pack 10, is provided with a raised pattern. A filter element 16 is arranged in the connecting cabin 17, and a reagent mixture obtained by reaction in the detection bag 10 enters the amplification cabin 18 after being filtered by the filter element 16. An amplification detection area of a transparent shell is arranged in the amplification cabin 18, and a semi-sealing area 13 is marked between the amplification detection area and the adjacent extraction partition 12. The interior of the amplification detection area is divided into at least one detection area which is mutually independent, and freeze-dried powder is filled in the detection area.
As shown in fig. 1, the fixing module comprises a fixing plate 1 and a reaction plate 2 which are buckled relatively, and the detection module is fixed between the fixing plate 1 and the reaction plate 2. A guide cavity is formed between the fixed plate 1 and the reaction plate 2, and the detection module is inserted into the guide cavity. One side surface of the fixing plate 1, which is close to the reaction plate 2, is marked as a joint surface, a U-shaped protruding guide rail is arranged along three side edges of the joint surface of the fixing plate 1, and after the fixing plate 1 and the reaction plate 2 are relatively jointed, the protruding guide rail is contacted with the reaction plate 2, so that a guide cavity is formed between the fixing plate 1 and the reaction plate 2. In the detection process, the fixed module is vertically placed, and the detection module is vertically inserted into the guide cavity from the top of the fixed module. The bottom of the guide cavity is provided with a sensing piece 6, the sensing piece 6 is touched after the detection module is completely inserted into the guide cavity, and the successful insertion of the detection module is confirmed by sensing.
A rectangular through groove is formed in the central area of the fixing plate 1, and the nucleic acid extraction area is exposed through the rectangular through groove after the detection module is inserted into the guide cavity. The lens 9 and the fixed block 8 are respectively embedded into the two side surfaces of the fixed plate 1, and the positions of the lens 9 and the fixed block 8 correspond. One side surface of the fixed plate 1, which is close to the reaction plate 2, is marked as an attaching surface, one side surface of the fixed plate 1, which is far away from the reaction plate 2, is marked as a detaching surface, the fixed block 8 is embedded into the attaching surface of the fixed plate 1, the fixed pressing block is used for pressing the nucleic acid amplification piece 19, the lens 9 is embedded into the detaching surface of the fixed plate 1, and optionally, the lens 9 is a biconvex lens 9.
The two side surfaces of the reaction plate 2 are respectively embedded with a heating member and a cooling member, the positions of the heating member and the cooling member correspond to each other, the heating member is used for heating the reagent mixture in the extracting member and the nucleic acid amplification member 19, and the cooling member is used for cooling the reagent mixture in the extracting member and the nucleic acid amplification member 19. One side of the reaction plate 2, which is close to the fixed plate 1, is marked as an attaching surface, one side of the reaction plate 2, which is far away from the fixed plate 1, is marked as a deviating surface, the heating element is embedded into the attaching surface of the reaction plate 2, and the cooling element is embedded into the deviating surface of the reaction plate 2. Specifically, the heating piece includes reaction heating board 5 and the amplification heating board 7 of embedding reaction board 2 faying face side by side, and after the fixed module was inserted to the detection module, reaction heating board 5 corresponded to the position of nucleic acid extraction piece, and amplification heating board 7 corresponded to the position of nucleic acid amplification piece 19, and reaction heating board 5 is used for heating the reagent mixture in the nucleic acid extraction district, and amplification heating board 7 is used for heating or refrigerating the reagent mixture in the amplification detection district. The cooling piece includes first fan 3 and the second fan 4 of embedding reaction plate 2 side by side face that deviates from, after the detection module inserts fixed module, first fan 3 corresponds with the position of nucleic acid extraction piece, second fan 4 corresponds with the position of nucleic acid amplification piece 19, first fan 3 is used for cooling the reagent mixture in the nucleic acid extraction district, second fan 4 is used for cooling the reagent mixture in the amplification detection district.
The motion module comprises a driving module, at least two compaction modules 27 and at least one extrusion module 28, wherein the compaction modules 27 and the extrusion modules 28 are transversely arranged side by side in the vertical direction, and the compaction modules 27 are respectively and correspondingly arranged in the compaction area 11 and the semi-sealing area 13. At least one extrusion module 28 is arranged between two adjacent compression modules 27, and the extrusion modules 28 correspond to the positions of the extraction partitions 12; before the detection starts, the driving module drives each compressing module 27 to fix the compressing area 11 and the semi-sealing area 13, so that each extracting partition 12 is relatively sealed; subsequently, the driving module drives each pressing module 28 to reciprocate in the horizontal direction, and repeatedly presses the corresponding extraction partition 12.
Each extraction partition 12 corresponds to two extrusion modules 28, the directions of reciprocating motions of the two extrusion modules 28 corresponding to the same extraction partition 12 are opposite, one extrusion module 28 is horizontally retracted and separated from the extraction partition 12, meanwhile, the other extrusion module 28 adjacent to the extraction partition 12 pushes out and extrudes the extraction partition 12, so that nucleic acid extraction reagent and a sample to be detected in the extraction partition 12 are fully mixed and extraction reaction is completed to obtain a reagent mixture, after the reaction in the extraction partition 12 is finished, a driving module drives a corresponding compression module 27 to separate from the semi-sealing area 13, the driving module drives a corresponding extrusion module to extrude the corresponding extraction partition 12, the extraction partition 12 is communicated with the next extraction partition 12 adjacent to the next extraction partition 12, and the reagent mixture flows into the next extraction partition 12.
The driving module comprises at least three groups of driving components which are transversely arranged side by side in the vertical direction, and each group of driving components is respectively and independently connected with the compressing module 27 or the extruding module 28 in a transmission manner. The driving assembly comprises a motor 21, a gear 23, a screw 24 and a motor guide shaft 26 which are sequentially connected in a transmission way, and the motor guide shaft 26 is connected with a pressing module 27 or an extruding module 28.
As shown in fig. 5, the compressing module 27 includes a compressing push plate guiding block 36, a compressing push plate connecting block 37 and a consumable compressing push plate guiding block 36 which are sequentially connected, and are in transmission connection with the motor guiding shaft 26, and the consumable compressing sheet 38 is in direct contact with the compressing area 11 or the semi-sealing area 13. The compressing push plate guide block 36 is connected with the compressing push plate connecting block through the snap spring shaft 31, a spring 32 is sleeved on the periphery of the snap spring shaft 31, and a snap spring 33 is arranged at the connecting end of the snap spring shaft 31 and the compressing push plate guide block 36.
As shown in fig. 4, the extrusion module 28 includes an extrusion push plate guide block 30, an extrusion push plate connecting block 34 and a consumable push plate 35 which are sequentially connected, the extrusion push plate guide block 30 is in transmission connection with the motor guide shaft 26, and the consumable push plate 35 is in direct contact with the extraction partition 12. The extrusion push plate guide block 30 is connected with an extrusion push plate connecting block through a clamp spring shaft 31, a spring 32 is sleeved on the periphery of the clamp spring shaft 31, and a clamp spring 33 is arranged at the connecting end of the clamp spring shaft 31 and the extrusion push plate guide block 30; the contact surface of the consumable pusher 35 and the extraction partition 12 is provided with a heating film, and the heating film heats the extraction partition 12.
As shown in fig. 3, the movement module further includes a module mounting case 25 of a groove structure, and the pressing module 27 and the pressing module 28 are placed in the module mounting case 25. The opening of the module mounting shell 25 is provided with a module pressing plate 29, and the module pressing plate 29 is used for fixing the compression module 27 and the extrusion module 28 in the module mounting shell 25. The movement module further comprises a motor mounting shell 22 of a groove structure, and the motor 21 is placed in the motor mounting shell 22. The opening of the motor mounting shell 22 is provided with a motor pressing plate 20, and the motor pressing plate 20 is used for fixing the motor 21 in the motor pressing plate 20.
In another embodiment, the present invention provides a detection method using the nucleic acid detecting apparatus provided in the above embodiment, the detection method comprising:
(1) Before the detection starts, the detection module is vertically arranged, a sample to be detected is injected into a first extraction partition 12 of the nucleic acid extraction area, and a compaction area 11 is marked between the first extraction partition 12 and a plastic nozzle 14; the semi-seal region 13 is marked between two adjacent extraction partitions 12 and between the nucleic acid amplification element 19 and the adjacent extraction partition 12;
(2) The driving modules respectively drive each pressing module 27 to be fixed to the pressing area 11 and the semi-sealing area 13, so that each extraction partition 12 forms a closed space, and detection begins;
(3) The corresponding driving module of the first extraction partition 12 drives the extrusion modules 28 to reciprocate in the horizontal direction, the directions of the reciprocating motions of the two corresponding extrusion modules 28 in the first extraction partition 12 are opposite, and the nucleic acid extraction reagent in the first extraction partition 12 and the sample to be detected are fully mixed under the heating of the reaction heating plate 5 to carry out nucleic acid extraction reaction;
(4) After the nucleic acid extraction reaction carried out in the first extraction partition 12 is finished, a reagent mixture is obtained, the driving module drives the corresponding compression module 27 to reset, the compression module 27 is separated from the semi-sealing area 13, the first extraction partition 12 is communicated with the adjacent second extraction partition 12 under the extrusion of the extrusion module 28, and the reagent mixture in the first extraction partition 12 flows into the second extraction partition 12;
(5) According to the operation logic of the step (4), after the extraction reaction in each extraction partition 12 is finished, the extraction partition is re-communicated with the semi-sealing area 13 between the adjacent next extraction partitions 12, so that the reagent mixture in the extraction partition 12 is pushed into the next extraction partition 12, the reagent mixture flows through each extraction partition 12 one by one and independently performs the nucleic acid extraction reaction in each extraction partition 12, different extraction partitions 12 are controlled to perform the extraction reaction at different temperatures until the last extraction partition 12 is reached, the extraction reaction is finished, and the first fan 3 and the second fan 4 are simultaneously started to perform air cooling and cooling on the nucleic acid extraction areas;
(6) After the extraction is finished, the semi-sealing area 13 between the last extraction partition 12 and the amplification detection area is re-communicated, the reagent mixture in the last extraction partition 12 enters the amplification detection area under the extrusion of the extrusion module 28, the amplification detection area is heated by the amplification heating plate 7, the reagent mixture is mixed with freeze-dried powder, the nucleic acid amplification reaction is finished, and after the amplification reaction is finished, the first fan 3 and the second fan 4 are simultaneously started to cool the amplification detection area, and the extrusion module 28 is reset.
The applicant declares that the above is only a specific embodiment of the present invention, but the scope of the present invention is not limited thereto, and it should be apparent to those skilled in the art that any changes or substitutions that are easily conceivable within the technical scope of the present invention disclosed by the present invention fall within the scope of the present invention and the disclosure.
Claims (34)
1. The full-automatic nucleic acid detection device is characterized by comprising a motion module and a detection module;
The detection module comprises a fixing module and a detection module, and the fixing module is used for fixing the detection module; the fixing module is provided with a heating piece and a cooling piece, and the heating piece and the cooling piece are used for heating and cooling the detection module;
the detection module comprises a nucleic acid extraction part and a nucleic acid amplification part fixed at one end of the nucleic acid extraction part, wherein the central area of the nucleic acid extraction part is a nucleic acid extraction area, a sample to be detected is injected into the nucleic acid extraction area before detection starts, and a semi-sealing area is marked between the nucleic acid extraction area and the nucleic acid amplification part;
the nucleic acid extraction area is divided into at least one extraction area;
at least one filtering area is arranged between the nucleic acid extraction area and the nucleic acid amplification piece, and filtering materials are filled in the filtering area;
the nucleic acid amplification part comprises an amplification cabin and a connection cabin communicated with the amplification cabin;
A filter element is arranged in the connecting cabin, and a reagent mixture obtained by reaction in the detection bag enters the amplification cabin after being filtered by the filter element; the motion module comprises a driving module, a compacting module and an extruding module, wherein the driving module is respectively and independently connected with the fixing module and the extruding module in a transmission way, the driving module drives the fixing module to compact the semi-sealing area, and the driving module drives the extruding module to extrude the nucleic acid extraction area to mix the nucleic acid extraction reagent in the nucleic acid extraction area with a sample to be detected;
The fixing module comprises a fixing plate and a reaction plate which are buckled relatively, and the detection module is fixed between the fixing plate and the reaction plate;
the two side surfaces of the reaction plate are respectively embedded with a heating piece and a cooling piece, the heating piece is used for heating the reagent mixture in the extracting piece and the nucleic acid amplification piece, and the cooling piece is used for cooling the reagent mixture in the extracting piece and the nucleic acid amplification piece;
the heating element comprises a reaction heating plate and an amplification heating plate which are embedded in parallel on the joint surface of the reaction plate, the reaction heating plate corresponds to the position of the nucleic acid extracting element after the detection module is inserted into the fixing module, the amplification heating plate corresponds to the position of the nucleic acid amplifying element, the reaction heating plate is used for heating the reagent mixture in the nucleic acid extracting area, and the amplification heating plate is used for heating or refrigerating the reagent mixture in the amplification detection area;
The cooling piece comprises a first fan and a second fan which are embedded in the deviating surface of the reaction plate side by side;
After the detection module is inserted into the fixing module, the first fan corresponds to the position of the nucleic acid extracting part, the second fan corresponds to the position of the nucleic acid amplifying part, the first fan is used for cooling the reagent mixture in the nucleic acid extracting area, and the second fan is used for cooling the reagent mixture in the amplifying detection area;
the compression modules and the extrusion modules are transversely arranged side by side in the vertical direction, the compression modules are respectively and correspondingly arranged in the compression area and the semi-sealing area, at least one extrusion module is arranged between two adjacent compression modules, and the extrusion modules correspond to the extraction areas in position; before the detection starts, the driving module drives each compressing module to fixedly compress the compressing area and the semi-sealing area, so that each extracting area is relatively sealed; then, the driving module drives each extrusion module to reciprocate in the horizontal direction, and the corresponding extraction partition is repeatedly extruded;
The extraction partition corresponds to two extrusion modules, the directions of the reciprocating motions of the two extrusion modules corresponding to the same extraction partition are opposite, one extrusion module is horizontally retracted and separated from the extraction partition, meanwhile, the other extrusion module adjacent to the extraction partition pushes out and extrudes the extraction partition, so that nucleic acid extraction reagent and a sample to be detected in the extraction partition are fully mixed and extraction reaction is completed to obtain a reagent mixture, after the reaction in the extraction partition is finished, the driving module drives the corresponding compression module to separate from the semi-sealing area, and the driving module drives the corresponding extrusion module to extrude the corresponding extraction partition, so that the extraction partition is communicated with the next adjacent extraction partition, and the reagent mixture flows into the next extraction partition.
2. The nucleic acid detecting apparatus according to claim 1, wherein the nucleic acid extracting member comprises a first fixing frame, a detecting bag and a second fixing frame, and the first fixing frame and the second fixing frame are fastened relatively to fix the detecting bag.
3. The nucleic acid detecting apparatus according to claim 2, wherein the nucleic acid amplification member is fixed to one end of the detection pack, and a plastic nozzle is fixed to the other end of the detection pack.
4. The nucleic acid detecting apparatus according to claim 2, wherein one end of the detecting bag is heat-sealed and fixed to the plastic nozzle, and the other end of the detecting bag is heat-sealed and fixed to the nucleic acid amplification element.
5. The nucleic acid detecting apparatus according to claim 4, wherein a plastic nozzle cover is provided at a nozzle opening of the plastic nozzle, and the plastic nozzle cover is used for sealing the nozzle opening of the plastic nozzle.
6. The nucleic acid detecting apparatus according to claim 5, wherein the plastic nozzle cover is fixed to the mouth of the plastic nozzle by direct insertion or screwing.
7. The nucleic acid detecting apparatus according to claim 1, wherein the central region of the detecting bag is a nucleic acid extraction region, a compression region is marked between the nucleic acid extraction region and the plastic mouth, and a semi-sealing region is marked between the nucleic acid extraction region and the nucleic acid amplification member.
8. The nucleic acid detecting apparatus according to claim 2, wherein one end of the detecting bag is wrapped around the connecting compartment, and the connecting compartment is fixedly connected to the detecting bag after the detecting bag is heat sealed with the connecting compartment.
9. The nucleic acid detecting apparatus according to claim 8, wherein the outer surface of the side of the connecting chamber which is in contact with the detecting bag is provided with a convex pattern.
10. The nucleic acid detecting apparatus according to claim 8, wherein an amplification detection region having a transparent casing is provided in the amplification chamber, and a semi-seal region is provided between the amplification detection region and the adjacent extraction region.
11. The nucleic acid detecting apparatus according to claim 1, wherein the amplification detection area is partitioned into at least one detection area which is independent of each other, and the detection area is filled with a lyophilized powder.
12. The nucleic acid detecting apparatus according to claim 1, wherein a guide cavity is formed between the fixing plate and the reaction plate, and the detecting module is inserted into the guide cavity.
13. The nucleic acid detecting apparatus according to claim 12, wherein a side surface of the fixing plate adjacent to the reaction plate is denoted as a fitting surface, and a U-shaped protruding rail is provided along three side edges of the fitting surface of the fixing plate, and the protruding rail is brought into contact with the reaction plate after the fixing plate and the reaction plate are fitted relatively, so that the guide cavity is formed between the fixing plate and the reaction plate.
14. The nucleic acid detecting apparatus according to claim 1, wherein the fixing module is vertically placed in the detecting process, and the detecting module is vertically inserted into the guide cavity from the top of the fixing module.
15. The nucleic acid detecting apparatus according to claim 13, wherein the bottom of the guide cavity is provided with a sensing member, and the detecting module is completely inserted into the guide cavity and then touches the sensing member, thereby sensing to confirm successful insertion of the detecting module.
16. The nucleic acid isolation detection device according to claim 1, wherein the center region of the fixing plate is provided with a rectangular through slot, and the nucleic acid isolation region is exposed through the rectangular through slot after the detection module is inserted into the guide cavity.
17. The nucleic acid detecting apparatus according to claim 16, wherein both side surfaces of the fixing plate are respectively embedded with a lens and a fixing block, and positions of the lens and the fixing block correspond to each other.
18. The nucleic acid detecting apparatus according to claim 17, wherein a side of the fixing plate adjacent to the reaction plate is denoted as a contact surface, a side of the fixing plate remote from the reaction plate is denoted as a separation surface, the fixing block is embedded in the contact surface of the fixing plate, the fixing pressing block is used for pressing the nucleic acid amplification element, and the lens is embedded in the separation surface of the fixing plate.
19. The nucleic acid detecting apparatus of claim 17, wherein the lens is a biconvex lens.
20. The nucleic acid detecting apparatus according to claim 1, wherein the heating member and the cooling member are positioned in correspondence.
21. The nucleic acid detecting apparatus according to claim 1, wherein a side of the reaction plate adjacent to the fixed plate is denoted as a contact surface, a side of the reaction plate remote from the fixed plate is denoted as a separation surface, the heating member is embedded in the contact surface of the reaction plate, and the cooling member is embedded in the separation surface of the reaction plate.
22. The nucleic acid extraction test apparatus according to claim 1, wherein the driving module comprises at least three driving modules arranged side by side in a vertical direction, and each driving module is independently and drivingly connected to the pressing module or the squeezing module.
23. The nucleic acid detecting apparatus of claim 22, wherein the driving assembly comprises a motor, a gear, a screw, and a motor guide shaft connected in series, and the motor guide shaft is connected to the pressing module or the extruding module.
24. The nucleic acid extraction detection device according to claim 23, wherein the compression module comprises a compression push plate guide block, a compression push plate connecting block and a consumable sheet which are sequentially connected, the compression push plate guide block is in transmission connection with the motor guide shaft, and the consumable sheet is in direct contact with the compression area or the semi-sealing area.
25. The nucleic acid detecting apparatus according to claim 22, wherein the pressing plate guide block is connected to the pressing plate connecting block through a snap spring shaft, a spring is provided around the outer circumference of the snap spring shaft, and a snap spring is provided at a connection end of the snap spring shaft and the pressing plate guide block.
26. The nucleic acid detecting apparatus according to claim 22, wherein the extrusion die set comprises an extrusion pusher guide block, an extrusion pusher connection block and a consumable pusher which are connected in sequence, the extrusion pusher guide block is in transmission connection with the motor guide shaft, and the consumable pusher is in direct contact with the extraction partition.
27. The nucleic acid detecting apparatus according to claim 22, wherein the push plate guide block is connected to the push plate connecting block via a snap spring shaft, a spring is provided around the snap spring shaft, and a snap spring is provided at a connection end of the snap spring shaft and the push plate guide block.
28. The nucleic acid detecting apparatus according to claim 26, wherein a heating film is provided on a contact surface between the consumable pusher plate and the extraction block, and the heating film heats the extraction block.
29. The nucleic acid detecting apparatus of claim 1, wherein the movement module further comprises a module mounting case having a groove structure, and the pressing module are placed in the module mounting case.
30. The nucleic acid detecting apparatus according to claim 1, wherein a die set pressing plate is provided at an opening of the die set mounting case, and the die set pressing plate is used for fixing the pressing die set and the extrusion die set in the die set mounting case.
31. The nucleic acid detecting apparatus as claimed in claim 1, wherein the movement module further comprises a motor mounting case having a groove structure, and the motor is placed in the motor mounting case.
32. The nucleic acid detecting apparatus as claimed in claim 31, wherein a motor pressing plate is provided at the opening of the motor mounting case, and the motor pressing plate is used for fixing the motor in the motor pressing plate.
33. A method of detecting a nucleic acid detecting apparatus according to any one of claims 1 to 32, comprising:
Before the detection starts, injecting a sample to be detected into the nucleic acid extraction area, marking a semi-sealing area between the nucleic acid extraction area and the nucleic acid amplification piece, driving the compression module to compress the semi-sealing area by the driving module, and driving the compression module to compress the nucleic acid extraction area by the driving module to mix the nucleic acid extraction reagent in the nucleic acid extraction area with the sample to be detected;
(II) the heating part heats the nucleic acid extraction area to complete the nucleic acid extraction reaction, and then the cooling part cools the nucleic acid extraction area; after the extraction reaction is finished, the compression module is separated from the semi-sealing area, the nucleic acid extraction area is communicated with the nucleic acid amplification piece, and the reagent mixture in the nucleic acid extraction area enters the nucleic acid amplification piece under the extrusion of the extrusion module;
(III) the heating part heats the nucleic acid amplification part to finish the nucleic acid amplification reaction, and the cooling part cools the nucleic acid amplification part after the amplification reaction is finished.
34. The method according to claim 33, wherein the method comprises the steps of:
(1) Before the detection starts, the detection module is vertically arranged, a sample to be detected is injected into a first extraction partition of the nucleic acid extraction area, and a compaction area is marked between the first extraction partition and the plastic nozzle; the half-seal area is marked between two adjacent extraction partitions and between the nucleic acid amplification part and the adjacent extraction partitions;
(2) The driving module drives each compression module to be fixed to the compression area and the semi-sealing area respectively, so that each extraction area forms a closed space, and detection is started;
(3) The driving module corresponding to the first extraction partition drives the extrusion modules to reciprocate in the horizontal direction, the directions of the reciprocating motions of the two extrusion modules corresponding to the first extraction partition are opposite, and the nucleic acid extraction reagent in the first extraction partition and the sample to be detected are fully mixed under the heating of the reaction heating plate to carry out nucleic acid extraction reaction;
(4) Obtaining a reagent mixture after the nucleic acid extraction reaction carried out in the first extraction partition, driving the corresponding compression module to reset by the driving module, separating the compression module from the semi-seal area, and re-communicating the first extraction partition with the adjacent second extraction partition under the extrusion of the compression module, wherein the reagent mixture in the first extraction partition flows into the second extraction partition;
(5) According to the operation logic of the step (4), after the extraction reaction in each extraction partition is finished, the extraction partition is re-communicated with a semi-sealing area between the adjacent next extraction partition, so that reagent mixture in the extraction partition is pushed into the next extraction partition, the reagent mixture flows through each extraction partition one by one and is subjected to nucleic acid extraction reaction in each extraction partition independently, different extraction partitions are controlled to carry out extraction reactions at different temperatures until the last extraction partition is reached, and a first fan and a second fan are started to carry out air cooling on the nucleic acid extraction areas simultaneously;
(6) After the extraction is finished, the semi-sealing area between the last extraction partition and the amplification detection area is communicated again, under the extrusion of the extrusion module, the reagent mixture in the last extraction partition enters the amplification detection area, the amplification detection area is heated by the amplification heating plate, the reagent mixture is mixed with freeze-dried powder, the nucleic acid amplification reaction is completed, and after the amplification reaction is finished, the first fan and the second fan are simultaneously started to cool the amplification detection area, and the extrusion module is reset completely.
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| CN206692844U (en) * | 2017-03-22 | 2017-12-01 | 张润鑫 | A kind of semi-automatic fastener sewing machine |
| CN107217001A (en) * | 2017-07-27 | 2017-09-29 | 广州和实生物技术有限公司 | A kind of detection of biological samples device and the gene tester based on the device |
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