CN112662522B - A atomizing device for cell culture liquid - Google Patents
A atomizing device for cell culture liquid Download PDFInfo
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- CN112662522B CN112662522B CN202110085699.6A CN202110085699A CN112662522B CN 112662522 B CN112662522 B CN 112662522B CN 202110085699 A CN202110085699 A CN 202110085699A CN 112662522 B CN112662522 B CN 112662522B
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- exhaust nozzle
- outer barrel
- fixedly connected
- inner cavity
- injection box
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- 238000004113 cell culture Methods 0.000 title claims abstract description 14
- 239000007788 liquid Substances 0.000 title description 6
- 238000002347 injection Methods 0.000 claims abstract description 64
- 239000007924 injection Substances 0.000 claims abstract description 64
- 239000000243 solution Substances 0.000 claims abstract description 20
- 239000000463 material Substances 0.000 claims abstract description 9
- 238000007789 sealing Methods 0.000 claims description 26
- 230000000903 blocking effect Effects 0.000 claims description 9
- 238000000889 atomisation Methods 0.000 claims description 8
- 238000005192 partition Methods 0.000 claims description 8
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims 4
- 235000017491 Bambusa tulda Nutrition 0.000 claims 4
- 241001330002 Bambuseae Species 0.000 claims 4
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims 4
- 239000011425 bamboo Substances 0.000 claims 4
- 239000012930 cell culture fluid Substances 0.000 claims 1
- 238000009826 distribution Methods 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 230000000694 effects Effects 0.000 description 4
- 238000013461 design Methods 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 241000883990 Flabellum Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229920001821 foam rubber Polymers 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000003116 impacting effect Effects 0.000 description 1
- 238000009688 liquid atomisation Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the technical field of atomizing devices, in particular to an atomizing device for cell culture solution, which comprises an outer barrel, wherein a tray is integrally connected in the outer barrel, an exhaust nozzle is fixedly connected in the middle of the tray and arranged between the outer barrel and the tray, a top cap is fixedly connected to the top of the outer barrel, an exhaust pipe is fixedly connected to one side of the top cap, the lower end of the top cap is communicated with an inner cavity of the outer barrel, a material injection box penetrates through a shell on one side of the outer barrel, and one end of the material injection box extends to the outside of the outer barrel.
Description
Technical Field
The invention relates to the technical field of atomization devices, in particular to an atomization device for cell culture solution.
Background
Cell culture liquid's atomizing device among the prior art through in packing into atomizing device with the culture solution, produces gaseous state cultivation fog, and such fog more is favorable to being absorbed by the bacterial colony, improves the culture efficiency of bacterial colony, and ordinary atomizing device work efficiency is low, carries out improved design to atomizing device and has the significance to the cell test.
If an atomization device can be invented, the atomization device has stable and efficient cell liquid atomization capability and is more sanitary and environment-friendly, the problem can be solved, and therefore the atomization device for the cell culture liquid is provided.
Disclosure of Invention
The present invention is directed to an apparatus for atomizing a cell culture solution, which solves the above problems of the prior art.
In order to achieve the purpose, the invention provides the following technical scheme: an atomizing device for cell culture solution comprises an outer barrel, wherein a tray is integrally connected in the outer barrel, an exhaust nozzle is fixedly connected in the middle of the tray and arranged between the outer barrel and the tray, a top cap is fixedly connected at the top of the outer barrel, an exhaust pipe is fixedly connected at one side of the top cap, the lower end of the top cap is communicated with an inner cavity of the outer barrel, an injection box penetrates through a shell at one side of the outer barrel, one end of the injection box extends to the outside of the outer barrel, an injection pipe is arranged at the end part of the injection box and fixed on the injection box and penetrates through the shell of the injection box, the other end of the injection box extends into the inner cavity of the outer barrel, a sealing door is arranged at the end part of the injection box, a central shaft penetrates through the end part of one end of the sealing door, a blocking block and a spring piece are arranged at one side of the central shaft, and the blocking block and the spring piece are arranged in the sealing door, the end part of the central shaft is fixedly connected with a side lug plate which is fixed on the material injection box, a plurality of hard pipes which are uniformly distributed are annularly arranged around the exhaust nozzle, the hard pipes penetrate through the shell of the exhaust nozzle, one end of each hard pipe extends into the inner cavity of the exhaust nozzle, the upper end of the exhaust nozzle is provided with a blocking ball, the lower end of the blocking ball is fixedly connected with a power rod, the lower end of the power rod extends into the inner cavity of the exhaust nozzle, the end part of the power rod is fixedly connected with a fan blade, the power rod is provided with a supporting seat, the lower end of the fan blade is provided with a convex disc, the convex disc is fixed on the wall of the inner cavity of the exhaust nozzle, the middle part of the convex disc penetrates through a control device, the end parts of a plurality of hard pipes in the exhaust nozzle are positioned between the control device and the fan blade, a partition plate is fixed in the inner cavity of the outer barrel at the upper end of the exhaust nozzle, and the upper end of the partition plate is contacted with a sponge cushion, the lower extreme of tray is provided with the gas injection pipe, and the one end of gas injection pipe extends to outer bucket outside, and the other end of gas injection pipe extends to the air discharge nozzle in, and the gas injection pipe runs through the casing of outer bucket and tray respectively.
Preferably, the control device comprises a top cover, a lifting column, a spring and a positioning barrel, wherein the lifting column penetrates through a through hole formed in the convex disc shell, the top cover is fixedly connected to the upper end of the lifting column, the spring and the positioning barrel are sleeved on the lifting column at the lower end of the convex disc, the positioning barrel is in contact with the lower end of the spring, the positioning barrel is fixedly sleeved on the lifting column, a plurality of L-shaped pipe cavities which are uniformly distributed are arranged in the lifting column, and the L-shaped pipe cavities are communicated with two inner cavities of an exhaust nozzle which is separated from the convex disc.
Preferably, the sealing door is in a shape that one side of a cylinder is integrally connected with a square plate, the middle part of the cylinder of the sealing door is movably sleeved with a central shaft, one side of the central shaft is fixedly connected with an intercepting block, one end of the intercepting block is contacted with a spring piece, and the intercepting block and the spring piece are arranged in an arc-shaped groove formed in the wall of an inner cavity of the cylinder of the sealing door.
Preferably, one side open-ended case of injection box is described as a shell, and sealing door shutoff injection box's open end, and the injection pipe runs through injection box's casing, the baffle shape is offered a plurality of evenly distributed's through-hole for the plectane and plate body, and the interception piece is fixed on the inner chamber wall of outer bucket.
Preferably, the intercepting ball is a ball shell body and is fixedly connected with a plurality of pyramids which are uniformly distributed on the lower half outer arc surface of the ball shell body, a plurality of clamping rings which are uniformly distributed are fixedly sleeved on the power rod, the supporting seat is in a shape of an L-shaped plate which is fixedly connected with two symmetrically distributed on the outer wall of the cylinder, and the L-shaped plate on the supporting seat is fixed on the exhaust nozzle.
Preferably, the power rod is sleeved in the cylinder of the supporting seat, the clamping ring is arranged in an annular groove formed in the wall of the inner cavity of the cylinder of the supporting seat in a matching mode, the lower end of the hard tube extends to the top of the inner cavity of the tray, and the exhaust nozzle is in a trapezoidal barrel shape.
Compared with the prior art, the invention has the beneficial effects that:
1. the structure design of the invention realizes stable and efficient atomization effect, namely, the air flow carrying water drops is sprayed out of the air discharge nozzle, the air flow impacts the rotating interception ball, and the rotating interception ball aggravates impact damage to the water drops, thereby realizing stable and efficient atomization effect;
2. the device passes through controlling means's design, ensures that the culture solution can not leak, and only when the air current passes through controlling means promptly, the air current jack-up top cap, and then through controlling means, other times are under the bounce-back promotion of spring, and the top cap is directly detained on protruding type dish, avoids the problem of leaking of culture solution, ensures whole atomizing device's clean health.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the exhaust nozzle;
fig. 3 is a schematic view of a tray structure.
In the figure: the device comprises an outer barrel 1, a tray 2, an exhaust nozzle 3, a top cap 4, an exhaust pipe 5, an injection box 6, an injection pipe 7, a sealing door 8, a central shaft 9, an interception block 10, a spring piece 11, a side lug plate 12, a hard pipe 13, an interception ball 14, a power rod 15, fan blades 16, a supporting seat 17, a convex disc 18, a control device 19, a partition plate 20, a sponge cushion 21, an injection pipe 22, a clamping ring 23, a top cover 24, a lifting column 25, a spring 26 and a positioning cylinder 27.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the technical solutions in the present invention, all other embodiments obtained by a person of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
Referring to fig. 1 to 3, the present invention provides a technical solution: an atomizing device for cell culture solution comprises an outer barrel 1, a tray 2 is integrally connected in the outer barrel 1, an exhaust nozzle 3 is fixedly connected in the middle of the tray 2, the exhaust nozzle 3 is arranged between the outer barrel 1 and the tray 2, a top cap 4 is fixedly connected on the top of the outer barrel 1, an exhaust pipe 5 is fixedly connected on one side of the top cap 4, the lower end of the top cap 4 is communicated with an inner cavity of the outer barrel 1, an injection box 6 penetrates through a shell on one side of the outer barrel 1, one end of the injection box 6 extends to the outside of the outer barrel 1, a material injection pipe 7 is arranged at the end part of the injection box 6, the material injection pipe 7 is fixed on the injection box 6, the material injection pipe 7 penetrates through the shell of the injection box 6, the other end of the injection box 6 extends into the inner cavity of the outer barrel 1, a sealing door 8 is arranged at the end part of the injection box 6, a central shaft 9 penetrates through the end part of the sealing door 8, a blocking block 10 and a spring piece 11 are arranged at one side of the central shaft 9, the interception block 10 and the spring leaf 11 are arranged in the sealing door 8, the end of the central shaft 9 is fixedly connected with a side ear plate 12, the side ear plate 12 is fixed on the material injection box 6, a plurality of hard pipes 13 which are uniformly distributed are annularly arranged around the exhaust nozzle 3, the hard pipes 13 penetrate through the shell of the exhaust nozzle 3, one end of each hard pipe 13 extends into the inner cavity of the exhaust nozzle 3, the upper end of the exhaust nozzle 3 is provided with an interception ball 14, the lower end of the interception ball 14 is fixedly connected with a power rod 15, the lower end of the power rod 15 extends into the inner cavity of the exhaust nozzle 3, the end of the power rod 15 is fixedly connected with a fan blade 16, the power rod 15 is provided with a support seat 17, the lower end of the fan blade 16 is provided with a convex disc 18, the convex disc 18 is fixed on the wall of the inner cavity of the exhaust nozzle 3, the middle part of the convex disc 18 penetrates through a control device 19, the ends of the hard pipes 13 in the exhaust nozzle 3 are positioned between the control device 19 and the fan blade 16, a partition plate 20 is fixed in an inner cavity of the outer barrel 1 at the upper end of the exhaust nozzle 3, a sponge pad 21 is contacted with the upper end of the partition plate 20, a gas injection pipe 22 is arranged at the lower end of the tray 2, one end of the gas injection pipe 22 extends to the outside of the outer barrel 1, the other end of the gas injection pipe 22 extends to the exhaust nozzle 3, the gas injection pipe 22 respectively penetrates through the shells of the outer barrel 1 and the tray 2, it can be understood by referring to fig. 1, one end of the gas injection pipe 22 is connected with a gas injection mechanism in the prior art, and gas is injected into the exhaust nozzle 3.
The control device 19 comprises a top cover 24, a lifting column 25, a spring 26 and a positioning barrel 27, wherein the lifting column 25 penetrates through a through hole formed in the shell of the convex disc 18, the top cover 24 is fixedly connected with the upper end of the lifting column 25, the lifting column 25 at the lower end of the convex disc 18 is sleeved with the spring 26 and the positioning barrel 27, the lower end of the spring 26 is contacted with the positioning barrel 27, the positioning barrel 27 is fixedly sleeved on the lifting column 25, a plurality of uniformly distributed L-shaped pipe cavities are arranged in the lifting column 25, and the L-shaped pipe cavities are communicated with two inner cavities of the exhaust nozzle 3 separated by the convex disc 18.
The shape of the sealing door 8 is that a square plate is integrally connected with one side of a cylinder, a central shaft 9 is movably sleeved in the middle of the cylinder of the sealing door 8, one side of the central shaft 9 is fixedly connected with an interception block 10, one end of the interception block 10 is contacted with a spring piece 11, and the interception block 10 and the spring piece 11 are arranged in an arc-shaped groove formed in the wall of an inner cavity of the cylinder of the sealing door 8.
One side of the injection box 6 is provided with an opening, the opening end of the injection box 6 is sealed by a sealing door 8, the injection pipe 7 penetrates through the shell of the injection box 6, the partition plate 20 is a circular plate and is provided with a plurality of uniformly distributed through holes, and the interception block 10 is fixed on the inner cavity wall of the outer barrel 1.
The intercepting ball 14 is in a ball shell shape, the lower half outer arc surface of the ball shell is fixedly connected with a plurality of pyramids which are uniformly distributed, the power rod 15 is fixedly sleeved with a plurality of clamping rings 23 which are uniformly distributed, the supporting seat 17 is in a shape of two L-shaped plates which are symmetrically distributed and fixedly connected to the outer wall of a cylinder, and the L-shaped plates on the supporting seat 17 are fixed on the exhaust nozzle 3.
The power rod 15 is sleeved in the cylinder of the supporting seat 17, the clamping ring 23 is arranged in an annular groove formed in the wall of the inner cavity of the cylinder of the supporting seat 17 in a matching mode, the lower end of the hard tube 13 extends to the top of the inner cavity of the tray 2, and the exhaust nozzle 3 is in a trapezoidal barrel shape. .
The working principle is as follows: the air current that injects in the air discharge nozzle 3 is discharged through the ending end at the top of the air discharge nozzle 3, and simultaneously the air current produces certain suction, and then store the culture solution in the tray 2, the culture solution is sucked into the air discharge nozzle 3 through the hard pipe 13, and then the air current drives the culture solution to rise and impact on the interception ball 14, the big water droplet that produces after so impacting falls into the tray 2 again, and the tiny water droplet rises, through the filtration of the foam-rubber cushion 21, namely very little water particle is discharged through the blast pipe 5 along with the air current, namely produces the effect of spraying, the air current rises in the air discharge nozzle 3 and drives the flabellum 16 to rotate, through the power pole 15 transmission interception ball 14, and then the interception ball 14 rotates and meets the water particle that spouts from the air discharge nozzle 3, improve the effect of broken water droplet, namely improve the efficiency of atomizing, in addition can be through injecting the culture solution into the injection box 6, the liquid pressure acts on the sealing door 8, certain rotation takes place for sealing door 8, and then during liquid flows into tray 2, and the gas injection process in the exhaust nozzle 3 to outer bucket 1, gas in the outer bucket 1 is used on sealing door 8, makes sealing door 8 automatic shutoff injection box 6, and gas can only be arranged outward through blast pipe 5 like this, realizes the guide control to the air current.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. An atomizing device for cell culture solution, comprising an outer tub (1), characterized in that: the inner cavity of the outer barrel (1) is integrally connected with a tray (2), the middle part of the tray (2) is fixedly connected with an exhaust nozzle (3), the exhaust nozzle (3) is arranged between the outer barrel (1) and the tray (2), the top of the outer barrel (1) is fixedly connected with a top cap (4), one side of the top cap (4) is fixedly connected with an exhaust pipe (5), the lower end of the top cap (4) is communicated with the inner cavity of the outer barrel (1), an injection box (6) penetrates through a shell on one side of the outer barrel (1), one end of the injection box (6) extends to the outside of the outer barrel (1), an injection pipe (7) is arranged at the end part of the injection box (6), the injection pipe (7) is fixed on the injection box (6), the injection pipe (7) penetrates through the shell of the injection box (6), the other end of the injection box (6) extends into the inner cavity of the outer barrel (1), and a sealing door (8) is arranged at the end part of the injection box (6), a central shaft (9) penetrates through the end part of one end of the sealing door (8), a blocking block (10) and a spring piece (11) are arranged on one side of the central shaft (9), the blocking block (10) and the spring piece (11) are arranged in the sealing door (8), a side ear plate (12) is fixedly connected to the end part of the central shaft (9), the side ear plate (12) is fixed on the material injection box (6), a plurality of uniformly distributed hard tubes (13) are annularly arranged around the exhaust nozzle (3), the hard tubes (13) penetrate through the shell of the exhaust nozzle (3), one end of each hard tube (13) extends into the inner cavity of the exhaust nozzle (3), a blocking ball (14) is arranged at the upper end of the exhaust nozzle (3), a power rod (15) is fixedly connected to the lower end of the blocking ball (14), the lower end of the power rod (15) extends into the inner cavity of the exhaust nozzle (3), and fan blades (16) are fixedly connected to the end part of the power rod (15), the improved air exhaust device is characterized in that a supporting seat (17) is arranged on the power rod (15), a convex disc (18) is arranged at the lower end of the fan blade (16), the convex disc (18) is fixed on the wall of an inner cavity of the air exhaust nozzle (3), a control device (19) penetrates through the middle of the convex disc (18), the end portions of a plurality of hard tubes (13) in the air exhaust nozzle (3) are located between the control device (19) and the fan blade (16), a partition plate (20) is fixed in an inner cavity of an outer barrel (1) at the upper end of the air exhaust nozzle (3), a sponge pad (21) is contacted with the upper end of the partition plate (20), a gas injection tube (22) is arranged at the lower end of the tray (2), one end of the gas injection tube (22) extends to the outside of the outer barrel (1), the other end of the gas injection tube (22) extends to the air exhaust nozzle (3), and the gas injection tube (22) respectively penetrates through shells of the outer barrel (1) and the tray (2).
2. An atomizing device for a cell culture solution according to claim 1, characterized in that: control device (19) include top cap (24), lift post (25), spring (26) and a location section of thick bamboo (27), the through-hole of seting up on protruding type dish (18) casing is run through in lift post (25), the upper end fixedly connected with top cap (24) of lift post (25), the cover has spring (26) and a location section of thick bamboo (27) on lift post (25) of protruding type dish (18) lower extreme, and the lower extreme contact of spring (26) has a location section of thick bamboo (27), and a location section of thick bamboo (27) fixed cover is on lift post (25), and the well is equipped with a plurality of evenly distributed's L type lumen in lift post (25), and two inner chambers of exhaust nozzle (3) that L type lumen intercommunication protruding type dish (18) cut off.
3. An atomizing device for a cell culture solution according to claim 1, characterized in that: the sealing door (8) is in the shape of a square plate integrally connected to one side of a cylinder, a central shaft (9) is movably sleeved in the middle of the cylinder of the sealing door (8), one side of the central shaft (9) is fixedly connected with an intercepting block (10), one end of the intercepting block (10) is contacted with a spring piece (11), and the intercepting block (10) and the spring piece (11) are arranged in an arc-shaped groove formed in the wall of an inner cavity of the cylinder of the sealing door (8).
4. An atomizing device for a cell culture solution according to claim 1, characterized in that: the opening of one side of injection box (6) is the case shell form, and sealing door (8) shutoff injection box (6) open end, and notes material pipe (7) run through the casing of injection box (6), baffle (20) shape is offered a plurality of evenly distributed's through-hole on plectane and the plate body, and intercepts piece (10) and fixes on the inner chamber wall of outer bucket (1).
5. An atomizing device for a cell culture solution according to claim 1, characterized in that: block ball (14) and be the spherical shell form and ball shell's the half outer cambered surface on fixedly connected with a plurality of evenly distributed's pyramid on the lower, the fixed cover has a plurality of evenly distributed's card position ring (23) on power rod (15), supporting seat (17) are the shape of the L template of two symmetric distributions of fixedly connected with on the drum outer wall, and the L template on supporting seat (17) is fixed on exhaust nozzle (3).
6. An atomization device for cell culture fluid according to claim 1, wherein: the power rod (15) is sleeved in the cylinder of the supporting seat (17), the clamping ring (23) is arranged in an annular groove formed in the wall of the inner cavity of the cylinder of the supporting seat (17) in a matching mode, the lower end of the hard pipe (13) extends to the top of the inner cavity of the tray (2), and the exhaust nozzle (3) is in a trapezoidal cylinder shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110085699.6A CN112662522B (en) | 2021-01-22 | 2021-01-22 | A atomizing device for cell culture liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110085699.6A CN112662522B (en) | 2021-01-22 | 2021-01-22 | A atomizing device for cell culture liquid |
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| Publication Number | Publication Date |
|---|---|
| CN112662522A CN112662522A (en) | 2021-04-16 |
| CN112662522B true CN112662522B (en) | 2022-06-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110085699.6A Active CN112662522B (en) | 2021-01-22 | 2021-01-22 | A atomizing device for cell culture liquid |
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| CN (1) | CN112662522B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113699022B (en) * | 2021-08-16 | 2024-01-26 | 上海知楚仪器有限公司 | Microorganism culture device and culture method thereof |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19520622C2 (en) * | 1995-06-06 | 2003-05-15 | Pari Gmbh | Device for atomizing fluids |
| CN2383592Y (en) * | 1999-06-30 | 2000-06-21 | 鹰潭市工业技术研究所 | Air-bag type atomizing apparatus |
| TWI233840B (en) * | 2003-04-14 | 2005-06-11 | Univ Nat Cheng Kung | Air-assisted-type singular atomization device |
| CN201172023Y (en) * | 2008-03-04 | 2008-12-31 | 中山大学 | Gas-liquid spray device |
| CN103657384A (en) * | 2013-12-26 | 2014-03-26 | 天津彩意科技有限公司 | Bubbler |
| CN105499047A (en) * | 2016-01-22 | 2016-04-20 | 苏州市计量测试研究所 | Novel aerosol atomizing device |
| CN106434303B (en) * | 2016-11-18 | 2019-04-23 | 天津开发区合普工贸有限公司 | Tissue Culture Dish bacterium infection experimental provision |
| CN206372977U (en) * | 2016-12-16 | 2017-08-04 | 上海野马环保设备工程有限公司 | A kind of air atomizing device of automatic imbibition |
| CN110433590A (en) * | 2019-08-08 | 2019-11-12 | 戴丽斯 | A kind of environmental project atomization plant |
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2021
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