CN112608875B - Perishable organic solid waste biological drying strain and application thereof - Google Patents

Abstract

The invention relates to the technical field of environmental microorganisms, in particular to a perishable organic solid waste biological drying treatment strain and its application, which has been identified as Geobacillus kaustophilus and named as Geobacillus kaustophilus FWGK‑ JYJ1, the deposit number is CCTCC NO:M 2020974. Geobacillus kaustophilus FWGK‑JYJ1 is resistant to high temperature, has strong self-generating heat capacity, and has the ability to quickly and efficiently release moisture in perishable organic solid waste. It can be used in catering waste, kitchen waste, fruit and vegetable waste, tail vegetables, The biological drying process of perishable organic solid waste such as livestock and poultry manure, municipal sludge, etc., realizes the effect of rapid drying and stabilization of perishable organic solid waste.

Description

A kind of perishable organic solid waste biological drying bacteria and its application

technical field

The invention relates to the technical field of environmental microorganisms, in particular to a perishable organic solid waste biological drying strain and its application.

Background technique

With the improvement of people's living standards and the continuous advancement of urbanization in our country, the production of perishable organic solid waste has increased year by year. Perishable organic solid waste mainly comes from agricultural waste, industrial waste and domestic waste. Especially since 46 cities in my country started the classification of domestic waste, the amount of perishable organic solid waste from domestic waste has risen sharply. The perishable organic solid waste has high moisture content, low calorific value, high organic content, and perishable odor, which brings certain difficulties to the collection, transportation and terminal treatment. If it is not treated effectively, it will easily lead to the reproduction of pathogenic bacteria, the breeding of mosquitoes and flies and water pollution, and it will also bring huge pressure to the living environment. .

At present, the commonly used perishable organic solid waste treatment technologies at home and abroad include dry incineration, sanitary landfill, anaerobic digestion, aerobic composting, and ecological feed. Each method has its own advantages and disadvantages: (1) Dry incineration: It can completely oxidize and decompose the organic components in the organic solid waste, and the reduction rate is as high as 50%-80%. However, due to the high moisture content of some organic solid wastes (such as kitchen waste), they are not suitable for direct incineration and must be pretreated by drying. (2) Sanitary landfill: The organic solid waste is buried underground, and various microorganisms are used to fully degrade macromolecules into small molecules. Its processing cost is low, the technology is simple, and it is suitable for various organic solid wastes, and is widely used in developing countries. However, there are major safety hazards. The generated methane and other gases may explode, and the leachate is likely to pollute the groundwater. At the same time, the resource recovery rate is basically zero, occupying a lot of land, and it is not suitable for areas with tight land use. (3) Anaerobic digestion: Under anaerobic conditions, the metabolism of facultative/anaerobic microorganisms is used to achieve volume reduction and resource utilization of organic solid waste. It has a high degree of automation, requires less manpower, is easy to control the emission of malodorous gases, has diversified products, and has high economic value. However, microorganisms have high requirements for pH, relatively complex treatment technology, and long biological start-up time in the reactor; the treatment of biogas slurry and biogas residue produced by anaerobic fermentation is still a major problem. (4) Aerobic composting: Under aerobic conditions, aerobic microorganisms are used to biodegrade the organic matter in the organic solid waste, and finally a stable high-fertility humus is formed. The technology is simple, easy to popularize, and high in resource utilization. However, the area is large, the composting process produces odor, and the economic benefit is not high. (5) Ecological feed: use some organic solid waste (such as kitchen waste) to ferment and produce ecological feed. It has developed better in Japan, and our country mainly produces bacterial protein by fermentation. Due to the potential food chain risks of food waste feed, it is necessary to operate with caution during production and use, and formulate corresponding industry standards and laws and regulations to ensure its safety. In general, the current perishable organic solid waste treatment technology still has problems such as low treatment efficiency, waste of resources, and serious secondary pollution.

Biological drying is a drying pretreatment technology for perishable organic solid waste that has emerged in recent years. Different from thermal drying, it does not need to consume external heat energy, but uses high-temperature aerobic microorganisms to metabolize biodegradable organic components in raw materials, decompose and release heat, and form a continuous and stable high-temperature environment (50-75%) in the drying chamber. ℃), the liquid water in the raw material is heated and evaporated into gaseous water, and then the water vapor in the drying chamber is discharged by the ventilation system, thereby reducing the moisture in the raw material. This method does not consume external heat energy, and can improve the low calorific value of raw materials, which is conducive to the recovery and utilization of heat energy, and can also partially stabilize the biodegradable organic matter, reducing the pollution potential of pollutants in the subsequent treatment process. The goal pursued by bio-drying technology is to achieve maximum water removal through minimum degradation of organic matter, and to rapidly reduce the moisture in organic materials. The organic solid waste with low moisture content obtained after biological drying pretreatment still retains most of the organic matter and has a high calorific value, which can be directly incinerated or further processed to prepare waste-derived fuel. However, the current biological drying of perishable organic solid waste still has technical problems such as slow start-up, short duration of high temperature, and poor drying effect. The main reason for this problem is that the indigenous microorganisms in the raw materials have high temperature, few species of high-efficiency functional microorganisms, low concentration and poor activity. Therefore, it is an urgent problem to screen functional microbial strains with high temperature resistance and strong self-heating ability to realize fast and efficient biological drying treatment of perishable organic solid waste.

SUMMARY OF THE INVENTION

In view of the above-mentioned shortcomings of the prior art, the purpose of the present invention is to provide a perishable organic solid waste biological drying strain and its application, which are used to solve the problems in the prior art.

In order to achieve the above object and other related objects, the present invention provides a Geobacillus kaustophilus FWGK -JYJ1, the deposit number is CCTCC NO: M 2020974.

The present invention also provides a liquid inoculum, the liquid inoculum comprises the Geobacillus kaustophilus FWGK-JYJ1, and the concentration of the Geobacillus kaustophilus FWGK -JYJ1 in the liquid inoculum is at least 1 ×10 ⁸ cfu/mL.

The present invention also provides a method for preparing the liquid inoculum, comprising the following steps: inoculating the pure strain of the Geobacillus kaustophilus FWGK-JYJ1 in a liquid medium for cultivation, and obtaining a liquid inoculum after the cultivation is completed .

The present invention also provides a solid inoculum comprising the Geobacillus kaustophilus FWGK-JYJ1 and a carrier.

The present invention also provides a method for preparing the solid inoculum, comprising the following steps: mixing the carrier and the liquid inoculum in a mass ratio of 1:1 to 1:10, and then drying.

The invention also provides the application of the Geobacillus kaustophilus FWGK-JYJ1 in the biological drying of perishable organic solid waste.

The invention also provides a method for biological drying of perishable organic solid waste, the method comprising the steps of: mixing the solid inoculum with the raw material of perishable organic solid waste and performing aerobic fermentation.

As mentioned above, a kind of perishable organic solid waste biological drying bacteria and application thereof of the present invention have the following beneficial effects:

(1) The Geobacillus kaustophilus FWGK-JYJ1 of the present invention has a strong self-producing heat capacity by aerobic fermentation, which can make the maximum temperature of the material reach 75 °C;

(2) The Geobacillus kaustophilus FWGK-JYJ1 described in the present invention has high temperature resistance, can grow normally under high temperature (50~75 ℃) conditions, and quickly dry perishable organic solid waste raw materials, The moisture content of perishable organic solid waste can be reduced from about 80% to about 40% within 20 hours;

(3) The Geobacillus kaustophilus FWGK-JYJ1 described in the present invention has the ability to tolerate high salt, and can perform biological drying treatment on perishable organic solid wastes such as food and beverage waste with high salt content.

Based on the above characteristics, Geobacillus kaustophilus FWGK-JYJ1 can be strengthened and applied to the biological drying treatment of perishable organic solid waste, solving the problems of high drying cost of perishable organic solid waste and long natural biological drying time. Harmless treatment and resource utilization of rot organic solid waste.

Description of drawings

Figure 1 shows the phylogenetic tree of the strain FWGK-JYJ1 of the present invention.

Figure 2 shows the change of water content in the process of biological drying of food waste by strain FWGK-JYJ1 of the present invention.

FIG. 3 shows the change of water content in the biological drying process of kitchen waste by the strain FWGK-JYJ1 of the present invention.

Figure 4 shows the change of water content in the biological drying process of fruit and vegetable waste by the strain FWGK-JYJ1 of the present invention.

Figure 5 shows the change of water content in the biological drying process of municipal sludge by strain FWGK-JYJ1 of the present invention.

FIG. 6 shows the change of water content in the biological drying process of mixed perishable organic solid waste by strain FWGK-JYJ1 of the present invention.

Detailed ways

The inventor obtained a high-efficiency perishable organic solid waste bio-drying strain through preliminary screening of the separation medium, re-screening of the strain growth temperature, and experimental verification of the perishable organic solid waste biological drying.

The first aspect of the present invention provides a perishable organic solid waste biological drying strain, which is identified as Geobacillus thermophilus through comprehensive analysis of data such as colony and cell morphology, physiological and biochemical characteristics, and 16S rDNA gene sequence determination. Geobacillus kaustophilus is deposited in CCTCC, China Collection Center for Type Cultures. The strain name is Geobacillus kaustophilus FWGK-JYJ1.

The perishable organic solid waste refers to the general term for organic solid wastes that are prone to decay and deterioration. Perishable organic solid waste is roughly divided into agricultural organic waste, industrial organic waste and municipal organic waste. Municipal organic waste mainly includes catering waste, kitchen waste, fruit and vegetable waste, livestock and poultry manure, municipal sludge, etc.

The Geobacillus kaustophilus FWGK-JYJ1 includes the gene sequence shown in SEQ ID NO.1.

The Geobacillus kaustophilus FWGK-JYJ1 is a gram-positive bacterium, rod-shaped, and the colony shape is round, pale yellow, with neat edges, and a smooth and shiny surface.

The Geobacillus kaustophilus FWGK-JYJ1 is resistant to high temperature and can grow normally under high temperature conditions of 50-75°C.

The aerobic fermentation of Geobacillus kaustophilus FWGK-JYJ1 self-produces heat. In one embodiment, the Geobacillus kaustophilus FWGK-JYJ1 enables the temperature of the material to reach 75°C.

In one embodiment, the Geobacillus kaustophilus FWGK-JYJ1 can be made into a liquid inoculum or a solid inoculum for biological drying.

The bio-drying treatment of perishable organic solid waste by Geobacillus kaustophilus FWGK-JYJ1, after 16-20 hours, the bio-drying rate is relative to the control group without Geobacillus kaustophilus FWGK -JYJ1 increased by more than 83%.

The biological drying treatment of perishable organic solid waste includes the following steps:

1) Add the solid inoculum of Geobacillus kaustophilus FWGK-JYJ1 to the perishable organic solid waste raw material, and the quality of the solid inoculum is 0.5% of the quality of the perishable organic solid waste raw material;

2) Aerobic fermentation for 16 h or 20 h, the material is stirred once per hour during the process, and the ventilation rate is 30 mL/min;

3) The moisture content of the material is determined by the drying method every 4 hours, and the biological drying rate is calculated using the formula (1).

In the above steps, the remaining operations are exactly the same except that the solid inoculum of Geobacillus kaustophilus FWGK-JYJ1 is not added in the control group.

Biological drying rate=(ω0-ω1)/ω0 (1)

In formula (1), ω0 is the initial moisture content of the material, and ω1 is the moisture content of the material at any time.

The increase in the biological drying rate of the experimental group (ie, containing Geobacillus kaustophilus FWGK-JYJ1) relative to that of the control group (ie, without Geobacillus kaustophilus FWGK-JYJ1) was calculated according to the following formula (2) Calculate:

The increase of the biological drying rate = (the biological drying rate of the experimental group - the biological drying rate of the control group)/the biological drying rate of the control group × 100% (2)

Perishable organic solid waste raw materials include food and beverage waste, kitchen waste, fruit and vegetable waste, livestock and poultry manure, and municipal sludge.

A second aspect of the present invention provides a liquid inoculum, the liquid inoculum comprises Geobacillus kaustophilus FWGK-JYJ1, and the concentration of Geobacillus kaustophilus FWGK -JYJ1 in the liquid inoculum is at least 1 ×10 ⁸ cfu/mL.

A third aspect of the present invention provides a method for preparing the liquid bacterial agent, which at least includes the following steps: inoculating a pure strain of Geobacillus kaustophilus FWGK-JYJ1 in a liquid medium for cultivation, and obtaining a liquid bacteria after the cultivation is completed. agent.

In one embodiment, the pure strain of Geobacillus kaustophilus FWGK-JYJ1 is inoculated into a liquid medium for cultivation, and then the obtained culture solution is inoculated into another liquid medium for expansion cultivation. Obtain a liquid inoculum.

In one embodiment, the temperature of the cultivation is 40-60°C.

In one embodiment, the stirring speed of the culture is 150-230 r/min.

In one embodiment, the pure strain of Geobacillus kaustophilus FWGK-JYJ1 is inoculated into a liquid medium for culturing for 12-16 hours, and then inoculated into another liquid medium for expansion culture.

In one embodiment, the liquid bacterial agent of the strain can be obtained by expanding the culture for 16-20 h.

The composition of liquid medium is: peptone 3 g/L, yeast extract 2 g/L, NaCl 3 g/L, MgCl ₂ 0.125 g/L, CaCl ₂ 0.5 g/L, FeSO ₄ 7H ₂ O 0.01 g/L , trace element solution (Na ₂ MoO ₄ 12 g/L, VOSO ₄ ·xH ₂ O1 g/L, MnCl ₂ 5 g/L, ZnSO ₄ ·7H ₂ O 0.6 g/L, CuSO ₄ ·5H ₂ O 0.15 g /L, CoCl ₂ ·6H ₂ O 8 g/L, NiCl ₂ ·6H ₂ O 0.2 g/L) 100 μL/L, pH was adjusted to 7~7.5.

A fourth aspect of the present invention provides a solid inoculum comprising the Geobacillus kaustophilus FWGK-JYJ1 and a carrier.

The carrier is a solid phase carrier. The solid phase carrier is, for example, starch, cyclodextrin, xanthan gum, wood chips, rice husk, and wheat bran.

A fifth aspect of the present invention provides a method for preparing the solid inoculum, comprising mixing and drying the carrier and the liquid inoculum in a mass ratio of 1:1 to 1:10.

The mass ratio of the carrier to the liquid bacterial agent is, for example, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9.

The drying conditions are drying the mixture to a moisture content of 10% or less.

The sixth aspect of the present invention provides the application of the Geobacillus kaustophilus FWGK-JYJ1 in the biological drying of perishable organic solid waste.

A seventh aspect of the present invention provides a method for biological drying of perishable organic solid waste, the method comprising the steps of: mixing the solid inoculum with the perishable organic solid waste raw material and performing aerobic fermentation.

In one embodiment, the quality of the solid bacterial agent is 0.1%-1% of the quality of the perishable organic solid waste raw material;

In one embodiment, aeration and agitation are performed during aerobic fermentation. Ventilation and stirring can ensure that the temperature of the material is uniform and carried out under aerobic conditions.

Aerobic fermentation time can be determined according to different materials. In one embodiment, the time for aerobic fermentation is 10 hours or more. The aerobic fermentation time is, for example, 10 to 15 hours, 15 to 20 hours, 20 to 25 hours or more.

The embodiments of the present invention are described below through specific specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention.

Before further describing the specific embodiments of the present invention, it should be understood that the protection scope of the present invention is not limited to the following specific specific embodiments; it should also be understood that the terms used in the examples of the present invention are for describing specific specific embodiments, Rather than limiting the scope of the invention; in the specification and claims of the present invention, the singular forms "a", "an" and "the" include the plural forms unless the context clearly dictates otherwise.

When numerical ranges are given in the examples, it is to be understood that, unless otherwise indicated herein, both endpoints of each numerical range and any number between the two endpoints may be selected. Unless otherwise defined, all technical and scientific terms used in the present invention have the same meaning as commonly understood by one of ordinary skill in the art. In addition to the specific methods, equipment and materials used in the embodiments, according to the mastery of the prior art by those skilled in the art and the description of the present invention, the methods, equipment and materials described in the embodiments of the present invention can also be used Any methods, devices and materials similar or equivalent to those of the prior art can be used to implement the present invention.

The following are the culture medium used in the embodiments of the present invention and its formulation:

Separation and purification medium: peptone 3 g/L, yeast extract 2 g/L, NaCl 3 g/L, MgCl ₂ 0.125 g/L, CaCl ₂ 0.5 g/L, FeSO ₄ 7H ₂ O 0.01 g/L, Trace element solution (Na ₂ MoO ₄ 12 g/L, VOSO ₄ ·xH ₂ O 1g/L, MnCl ₂ 5 g/L, ZnSO ₄ ·7H ₂ O 0.6 g/L, CuSO ₄ ·5H ₂ O 0.15 g/L L, CoCl ₂ ·6H ₂ O 8 g/L, NiCl ₂ ·6H ₂ O 0.2 g/L) 100 μL/L, agar 20 g/L, pH adjusted to 7~7.5.

Seed medium: peptone 3 g/L, yeast extract 2 g/L, NaCl 3 g/L, MgCl ₂ 0.125 g/L, CaCl ₂ 0.5 g/L, FeSO ₄ 7H ₂ O 0.01 g/L, trace Elemental solution (Na ₂ MoO ₄ 12 g/L, VOSO ₄ ·xH ₂ O 1 g/L, MnCl ₂ 5 g/L, ZnSO ₄ ·7H ₂ O 0.6 g/L, CuSO ₄ ·5H ₂ O 0.15 g/L L, CoCl ₂ ·6H ₂ O 8 g/L, NiCl ₂ ·6H ₂ O 0.2 g/L) 100 μL/L, and the pH value was adjusted to 7~7.5.

Liquid medium: peptone 3 g/L, yeast extract 2 g/L, NaCl 3 g/L, MgCl ₂ 0.125 g/L, CaCl ₂ 0.5 g/L, FeSO ₄ 7H ₂ O 0.01 g/L, trace Elemental solution (Na ₂ MoO ₄ 12 g/L, VOSO ₄ ·xH ₂ O 1 g/L, MnCl ₂ 5 g/L, ZnSO ₄ ·7H ₂ O 0.6 g/L, CuSO ₄ ·5H ₂ O 0.15 g/L L, CoCl ₂ ·6H ₂ O 8 g/L, NiCl ₂ ·6H ₂ O 0.2 g/L) 100 μL/L, and the pH value was adjusted to 7~7.5.

After the above media were prepared, they were all sterilized by autoclaving at 121 °C for 15 min.

Example 1: Isolation, screening and performance determination of strain FWGK-JYJ1

The separation and purification process of the strain FWGK-JYJ1 of the present invention: the test sample is collected from a high-temperature aerobic fermentation tank of a food and beverage waste treatment plant, and after adding sterile water and mixing, gradient dilution is performed, and 200 μL of different gradient dilutions are spread on After culturing in a constant temperature incubator at 50 °C for 24-48 h on the separation and purification medium plate, single colonies with different shapes and sizes were picked, streaked and purified, and numbered for storage. A total of 41 strains were obtained through primary screening.

The strains obtained from the primary screening were screened at the highest growth temperature, and the strains were streaked on the solid medium and placed in an incubator with a temperature gradient of 50, 55, 60, 65, 70, 75, and 80 °C, and their growth was recorded. In some cases, the high temperature resistant strains with the highest growth temperature above 60°C were screened, and a total of 15 strains were obtained after rescreening.

The strains obtained by re-screening were inoculated into the seed medium, cultured at 50 °C, 180 r/min for 16 h, and then transferred to the same liquid medium with 1% inoculum for expansion culture, at 50 °C, 180 r/min. After culturing for 16-20 h, the liquid inoculum of the strain can be obtained. Take 200 mL of the liquid bacterial agent, wash it with sterile saline after centrifugation, resuspend it to 5 mL, add it to 1000 g of food waste (taken from the canteen of the Shanghai Institutes for Advanced Study, Chinese Academy of Sciences), stir well, and conduct a biological drying experiment. Oxygen fermentation, the material was stirred once per hour during the process, and the ventilation rate was 30 mL/min. The results showed that the drying effect of the food waste inoculated with the strain FWGK-JYJ1 was the most obvious, and the moisture content decreased from the initial moisture content of 81% to 38% at 24 h, that is, the biological drying rate was 53.1%.

The main biological characteristics of strain FWGK-JYJ1 are Gram-positive bacteria, rod-shaped, and the colony shape is round, light yellow, neat edge, smooth and shiny surface. The maximum temperature tolerance of strain FWGK-JYJ1 is 75 ℃, and it does not grow when it is higher than 75 ℃. The 16S rDNA sequence of the strain is shown in SEQ ID NO. 1. The 16S rDNA sequence was submitted to NCBI, and the homology sequence alignment analysis was performed with the software and GenBank, and the MEGA X software was used to construct the phylogenetic tree of the strain (Figure 1). . Based on the above information, the strain FWGK-JYJ1 was identified as Geobacillus kaustophilus .

Example 2: Preparation of bacterial agent of strain FWGK-JYJ1

Preparation of liquid inoculum: FWGK-JYJ1 pure strain was inoculated into five 10 mL seed medium, cultured in a constant temperature shaker at 50 °C and 180 r/min for 12 h, and then added to 1 volume of liquid medium. % of the inoculum was transferred into 5 L liquid medium for expanded culture, and cultured at 50 °C and 180 r/min for 18 h to obtain the liquid inoculum of the strain.

Preparation of solid inoculum: The starch and the liquid inoculum of the strain are mixed in a mass ratio of 1:5 and prepared by spray drying.

Example 3: Bio-drying treatment effect of strain FWGK-JYJ1 on single perishable organic solid waste raw materials from different sources

The solid inoculum of Geobacillus thermophilus FWGK-JYJ1 is added to the single perishable organic solid waste raw material from different sources. The amount of the solid inoculum is 0.5% of the mass of the raw material. After stirring evenly, aerobic fermentation is carried out, and ventilation is required during the process. , stirring to ensure uniform temperature and under aerobic conditions. The moisture content of the material is measured every 4 h, and the moisture content change of the material is obtained, and the moisture content change is shown in Figures 2 to 5.

The food waste was taken from the canteen of the Shanghai Institutes for Advanced Study, Chinese Academy of Sciences, and its moisture content was 80.2%. Figure 2 shows the effect of Geobacillus thermophilus FWGK-JYJ1 on changes in the moisture content of food waste. It can be seen from the figure that the change of the moisture content of the food and beverage waste after the addition of Geobacillus thermophilus FWGK-JYJ1 reached the minimum value of 38.6% in 16 h, and the minimum value of the moisture content of the control group without the addition of inoculants was Compared with the control group, the biological drying rate of the experimental group increased by 90.8%. It can be seen that the access to Geobacillus thermophilus FWGK-JYJ1 can significantly improve the biological drying effect of food waste.

The kitchen waste was taken from the canteen of the Shanghai Institutes for Advanced Study of the Chinese Academy of Sciences, and its moisture content was 76.8%. Figure 3 shows the effect of Geobacillus thermophilus FWGK-JYJ1 on the change of moisture content of kitchen waste. It can be seen from the figure that the change of moisture content of kitchen waste after adding Geobacillus thermophilus FWGK-JYJ1 to the kitchen waste after 16 h It reached the minimum value of 32.4%, while the minimum moisture content of the control group without inoculants was 52.6%, and the biological drying rate of the experimental group was 83.5% higher than that of the control group. It can be seen that the access to Geobacillus thermophilus FWGK-JYJ1 can significantly improve the biological drying effect of kitchen waste.

The fruit and vegetable waste was taken from a farmer's market in Shanghai, and its moisture content was 91.3%. Figure 4 shows the effect of Geobacillus thermophilus FWGK-JYJ1 on the change of moisture content of fruit and vegetable waste. It can be seen from the figure that the change of moisture content of fruit and vegetable waste after adding Geobacillus thermophilus FWGK-JYJ1 to fruit and vegetable waste reached 20 h. The minimum value is 47.6%, while the minimum moisture content of the control group without the addition of bacterial agent is 67.9%, and the biological drying rate of the experimental group is 86.8% higher than that of the control group. It can be seen that the access to Geobacillus thermophilus FWGK-JYJ1 can significantly improve the biological drying effect of fruit and vegetable waste.

Municipal sludge was taken from a sewage treatment plant in Shanghai, and its moisture content was 79.9%. Figure 5 shows the effect of Geobacillus thermophilus FWGK-JYJ1 on the change of water content of municipal sludge. It can be seen from the figure that the change of water content of municipal sludge caused by the addition of Geobacillus thermophilus FWGK-JYJ1 was within 20 h. It reached the minimum value of 41.3%, while the minimum moisture content of the control group without inoculants was 60.1%, and the biological drying rate of the experimental group was 95.0% higher than that of the control group. It can be seen that the access to Geobacillus thermophilus FWGK-JYJ1 can significantly improve the biological drying effect of municipal sludge.

In conclusion, the access to Geobacillus thermophilus FWGK-JYJ1 can realize the rapid drying of perishable organic solid waste, so as to achieve the effects of harmlessness, stabilization and reduction of perishable organic solid waste.

Example 4: Biological drying treatment effect of strain FWGK-JYJ1 on mixed perishable organic solid waste

Mix the three kinds of perishable organic solid wastes, such as kitchen waste, tail vegetables and livestock and poultry manure, according to the mass ratio of 1:1:1. The moisture content of the mixed material is 77.7%. Add Geobacillus thermophilus FWGK-JYJ1 solid inoculum to the waste. The amount of solid inoculum is 1% of the raw material mass. After stirring evenly, aerobic fermentation is carried out. During the process, ventilation and stirring are required to ensure uniform temperature. performed under aerobic conditions. The moisture content of the materials in the treatment system was measured every 4 h, and the moisture content changes of the materials were obtained, as shown in Figure 6. The results showed that the change of water content of mixed perishable organic solid waste by adding Geobacillus thermophilus FWGK-JYJ1 reached the minimum value of 31.1% in 12 h, while the minimum value of water content in the control group was 55.3%. The biological desiccation rate of the group increased by 108.0%. It can be seen that Geobacillus thermophilus FWGK-JYJ1 can be used to degrade mixed perishable organic solid wastes such as kitchen waste, tail vegetables, livestock and poultry manure under aerobic conditions, and has high application value and broad application prospects. .

The results of the biological drying experiment of the strain show that the strain can achieve rapid drying of perishable organic solid waste under aerobic conditions, achieving the purpose of harmlessness, reduction and stabilization, and can be used for the treatment of catering waste and kitchen waste. , fruit and vegetable waste and other perishable organic solid waste, with high application value.

The above examples are intended to illustrate the disclosed embodiments of the present invention, and should not be construed as limiting the present invention. Furthermore, various modifications set forth herein and variations in the methods of the invention will be apparent to those skilled in the art without departing from the scope and spirit of the invention. Although the present invention has been described in detail in conjunction with various specific preferred embodiments of the present invention, it should be understood that the present invention should not be limited to these specific embodiments. Indeed, various modifications as described above which are obvious to those skilled in the art to obtain the invention are intended to be included within the scope of the present invention.

sequence listing

<110> Shanghai Institutes for Advanced Study, Chinese Academy of Sciences

<120> A kind of perishable organic solid waste biological drying bacteria and its application

<160> 1

<170> SIPOSequenceListing 1.0

<210> 1

<211> 1432

<212> DNA

<213> Geobacillus kaustophilus FWGK-JYJ1

<400> 1

gctggctccc gtaagggtta cctcaccgac ttcgggtgtt gcaagctctc gtggtgtgac 60

gggcggtgtg tacaaggccc gggaacgtat tcaccgcggc atgctgatcc gcgattacta 120

gcgattccgg cttcatgcag gcgagttgca gcctgcaatc cgaactgaga gcggcttttt 180

gggattcgct ccccctcgcg ggttcgcagc cctttgtacc gcccattgta gcacgtgtgt 240

agcccaggtc ataaggggca tgatgatttg acgtcatccc caccttcctc cgacttgtcg 300

ccggcagtcc ctctagagtg cccaccttcg tgctggcaac tagaggcgag ggttgcgctc 360

gttgcgggac ttaacccaac atctcacgac acgagctgac gacaaccatg caccacctgt 420

caccctgtcc ccccgaaggg ggaacgccca atctcttggg ttgtcagggg atgtcaagac 480

ctggtaaggt tcttcgcgtt gcttcaaatt aaaccacatg ctccaccgct tgtgcgggcc 540

cccgtcaatt cctttgagtt tcagccttgc ggccgtactc cccaggcgga gtgcttatcg 600

cgttagctgc agcactaaag ggtgtgaccc ctctaacact tagcactcat cgtttacggc 660

gtggactacc agggtatcta atcctgtttg ctccccacgc tttcgcgcct cagcgtcagt 720

tgcaggccag agagccgcct tcgccactgg tgttcctcca catctctacg catttcaccg 780

ctacacgtgg aattccgctc tcctctcctg cactcaagtc ccccagtttc caatgaccct 840

ccacggttga gccgtgggct ttcacatcag acttaaggaa ccgcctgcgc gcgctttacg 900

cccaataatt ccggacaacg ctcgccccct acgtattacc gcggctgctg gcacgtagtt 960

agccggggct tcctcgtgag gtaccgtcac cgcgccgccc tcttcgaacg gcgctccttc 1020

gtccctcaca acagagcttt acgacccgaa ggccttcttc gctcacgcgg cgtcgctccg 1080

tcaggctttc gcccattgcg gaagattccc tactgctgcc tcccgtagga gtctgggccg 1140

tgtctcagtc ccagtgtggc cggtcaccct ctcaggccgg ctacgcatcg tcgccttggt 1200

gagccgttac ctcaccaact agctaatgcg ccgcgggccc atccgcaagt gacagccaaa 1260

ggccgccttt caaccaaaga ccatgcggtc ttcggtgtta tccggtatta gctccggttt 1320

cccggagtta tcccggtctt gcgggcaggt tgcccacgtg ttactcaccc gtccgccgct 1380

gaccaaatca gagcaagctc cgatttggtc cgctcgactt gcatgtatta gc 1432

Claims (12)

1. A Geobacillus kaustophilus FWGK-JYJ1, the deposit number is CCTCCNO: M 2020974. 2 . The Geobacillus kaustophilus FWGK-JYJ1 according to claim 1 , wherein the Geobacillus kaustophilus FWGK-JYJ1 comprises the SEQ ID NO.1 gene sequence. 3 . The Geobacillus kaustophilus FWGK-JYJ1 according to claim 1 , wherein the Geobacillus kaustophilus FWGK-JYJ1 can withstand a high temperature of 75° C. 4 . 4. The Geobacillus kaustophilus FWGK-JYJ1 according to claim 1, wherein the Geobacillus kaustophilus FWGK-JYJ1 is mixed with perishable organic solid waste and mixed well Oxygen fermentation for 16-20 hours can increase the biological drying rate of perishable organic solid waste by more than 83%. 5. A liquid microbial inoculum, wherein the liquid microbial inoculum comprises the thermophilic Geobacillus kaustophilus FWGK-JYJ1 as described in any one of claims 1-4, and the liquid microbial inoculum is thermophilic. The concentration of Geobacillus kaustophilus FWGK-JYJ1 is at least 1×10 ⁸ cfu/mL. 6. The preparation method of the liquid inoculum according to claim 5, characterized in that it comprises the steps of: inoculating the pure strain of Geobacillus kaustophilus FWGK-JYJ1 according to any one of claims 1-4 Cultivated in a liquid medium, and obtained a liquid bacterial agent after the cultivation. 7. A solid inoculum, characterized in that the solid inoculum comprises the Geobacillus kaustophilus FWGK-JYJ1 as described in any one of claims 1-4 and a carrier. 8. The solid inoculum according to claim 7, wherein the carrier is a solid phase carrier. 9. the preparation method of the arbitrary described solid microbial inoculum of claim 7-8, is characterized in that, comprises the steps: the carrier and the described liquid microbial inoculum of claim 5 are mixed according to mass ratio 1:1～1:10 After drying. 10. The application of Geobacillus kaustophilus FWGK-JYJ1 according to any one of claims 1-4 in the biological drying of perishable organic solid waste. 11. A method for biological drying of perishable organic solid waste, characterized in that the method comprises the steps of: mixing the solid bacterial agent according to claim 7 or 8 with the raw material of perishable organic solid waste and carrying out aerobic Fermentation. 12 . The method according to claim 11 , wherein the perishable organic solid waste comprises food and beverage waste, kitchen waste, fruit and vegetable waste, tail vegetables, livestock and poultry manure or municipal sludge. 13 .

Images