CN106587559A - Sludge anaerobic digestion method - Google Patents
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- 239000010802 sludge Substances 0.000 title claims abstract description 63
- 230000029087 digestion Effects 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 25
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims abstract description 48
- 238000006243 chemical reaction Methods 0.000 claims abstract description 16
- 239000007787 solid Substances 0.000 claims abstract description 16
- -1 rare earth nitrate Chemical class 0.000 claims abstract description 12
- 229910052761 rare earth metal Inorganic materials 0.000 claims abstract description 11
- 238000003756 stirring Methods 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000002054 inoculum Substances 0.000 claims abstract description 7
- 241000894006 Bacteria Species 0.000 claims description 30
- 239000007788 liquid Substances 0.000 claims description 17
- 244000005700 microbiome Species 0.000 claims description 15
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 14
- 241000194035 Lactococcus lactis Species 0.000 claims description 14
- 241000589597 Paracoccus denitrificans Species 0.000 claims description 13
- 235000014897 Streptococcus lactis Nutrition 0.000 claims description 13
- 229910017604 nitric acid Inorganic materials 0.000 claims description 13
- 238000002360 preparation method Methods 0.000 claims description 13
- 241000194103 Bacillus pumilus Species 0.000 claims description 12
- 241000588697 Enterobacter cloacae Species 0.000 claims description 12
- 230000000855 fungicidal effect Effects 0.000 claims description 6
- 239000000417 fungicide Substances 0.000 claims description 6
- 239000002068 microbial inoculum Substances 0.000 claims description 6
- 239000005995 Aluminium silicate Substances 0.000 claims description 5
- 235000012211 aluminium silicate Nutrition 0.000 claims description 5
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims description 5
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 3
- 241001633977 Paracoccus pantotrophus Species 0.000 claims description 3
- HSJPMRKMPBAUAU-UHFFFAOYSA-N cerium(3+);trinitrate Chemical compound [Ce+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O HSJPMRKMPBAUAU-UHFFFAOYSA-N 0.000 claims description 3
- 210000003608 fece Anatomy 0.000 claims description 3
- 239000012467 final product Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 238000003672 processing method Methods 0.000 claims description 3
- FYDKNKUEBJQCCN-UHFFFAOYSA-N lanthanum(3+);trinitrate Chemical compound [La+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O FYDKNKUEBJQCCN-UHFFFAOYSA-N 0.000 claims description 2
- 239000010871 livestock manure Substances 0.000 claims description 2
- 239000013049 sediment Substances 0.000 claims description 2
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 8
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- 150000001875 compounds Chemical class 0.000 abstract 1
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- 239000010865 sewage Substances 0.000 description 9
- 239000000126 substance Substances 0.000 description 9
- 230000008569 process Effects 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
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- 239000002253 acid Substances 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- 150000007524 organic acids Chemical class 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 2
- 229910002492 Ce(NO3)3·6H2O Inorganic materials 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
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- 238000007796 conventional method Methods 0.000 description 2
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- 238000006297 dehydration reaction Methods 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- SNRUBQQJIBEYMU-UHFFFAOYSA-N dodecane Chemical compound CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
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- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- DUWWHGPELOTTOE-UHFFFAOYSA-N n-(5-chloro-2,4-dimethoxyphenyl)-3-oxobutanamide Chemical compound COC1=CC(OC)=C(NC(=O)CC(C)=O)C=C1Cl DUWWHGPELOTTOE-UHFFFAOYSA-N 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 235000019260 propionic acid Nutrition 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000305071 Enterobacterales Species 0.000 description 1
- 229910002422 La(NO3)3·6H2O Inorganic materials 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 238000011953 bioanalysis Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 210000003555 cloaca Anatomy 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- DIOQZVSQGTUSAI-NJFSPNSNSA-N decane Chemical group CCCCCCCCC[14CH3] DIOQZVSQGTUSAI-NJFSPNSNSA-N 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000010954 inorganic particle Substances 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000696 methanogenic effect Effects 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 229910052901 montmorillonite Inorganic materials 0.000 description 1
- DIOQZVSQGTUSAI-UHFFFAOYSA-N n-butylhexane Natural products CCCCCCCCCC DIOQZVSQGTUSAI-UHFFFAOYSA-N 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000001473 noxious effect Effects 0.000 description 1
- 125000001477 organic nitrogen group Chemical group 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
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- 244000052769 pathogen Species 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000011112 process operation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000010801 sewage sludge Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 235000012222 talc Nutrition 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
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- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
- C02F11/04—Anaerobic treatment; Production of methane by such processes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/20—Sludge processing
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Organic Chemistry (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention relates to a sludge anaerobic digestion method. The sludge anaerobic digestion method comprises the following steps: (1) adjusting the solid content of sludge to be 5-10%; (2) adding the sludge to an anaerobic digestion tank, wherein the addition quantity of the sludge accounts for 60% (v/v) of the volume of the digestion tank, adding a methane production inoculum, the addition quantity of which accounts for 8-10% (v/v) of the volume of the digestion tank, adding water till reaching 90% of the volume of the digestion tank, and adjusting the pH value to 7.5-7.7; (3) inoculating a compound microbial agent, the addition quantity of which accounts for 0.5-1.2% (v/v) of the volume of the digestion tank, uniformly stirring and covering; and (4) maintaining the reaction temperature at about 25-35 DEG C, stirring once every 10 hours, adding rare earth nitrate while stirring, and reacting for 36 hours. The sludge anaerobic digestion method has the beneficial effects that the amount of methane produced from the same amount of sludge can be greatly increased, the efficiency is well improved, the utilization rate of sludge is increased, and the sludge is better recycled.
Description
Technical field
The invention belongs to environmental technology field, and in particular to a kind of method of anaerobic sludge digestion.
Background technology
As Urbanization in China and town development paces are accelerated and population increase, the public is wanted to environmental quality
Ask and increasingly improve, substantial amounts of sewage treatment plant constantly builds up and comes into operation, thing followed sludge quantity is also increasing.Sludge
The product after sewage disposal, be it is a kind of by organic relic, microorganism, inorganic particle, colloid etc. constitute it is extremely complex
Heterogeneous body, the key property of sludge is that moisture content is high, and content of organics is high, easily degenerates smelly, and granule is thinner, proportion
It is less, jelly liquid, it is the concentrated thing between liquid and solid, can be transported with pump, but it is difficult by sedimentation
Carry out solid-liquid separation.Although the annual production statistics in industry for sludge is not reached common understanding at present, scale is quite big,
But for a long time, there is wastewater treatment emphatically in China, the tendency of light Treatment of Sludge, current China's specific resistance to filtration mainly have
The conventional process patterns such as landfill, burning, throwing sea and Land_use change.In sewage treatment plant, sludge processes directly agricultural without any
More than 60% is accounted for, even if being provided with the sewage treatment plant of digester, postdigestive sludge is also slightly dehydration with regard to direct agriculture
With or landfill.Although country takes a large amount of human and material resources and financial resources have processed sewage, due to process without appropriate these
After sludge enters environment, secondary pollution is brought directly to water body and air, serious prestige is constituted to ecological environment and mankind's activity
The side of body.So the minimizing, stabilisation, the research of innoxious and resource and practice to sludge all becomes particularly important and urgent.
Generally Treatment of Sludge expense accounts for the 20%～40% of sewage treatment plant's investment and operating cost, and anaerobic sludge digestion
It is sludge organism processing method conventional in the world, meanwhile, and the Treatment of Sludge method that large-scale wastewater treatment plant is the most economic.
It is a multilevel process, i.e., the Organic substance (such as protein, fat, carbohydrate) in sludge is hydrolyzed and in souring stage
Short chain shape organic acid, alcohol, H2, CO2 and acetic acid are degraded into by different facultative floras, wherein organic acid and alcohol is further decomposed into
Acetic acid, ultimately produces biogas.Thus, sludge has reached stabilisation and innoxious, and recyclable bioenergy.Mud is disappeared by anaerobism
Change, the volume of sludge can be made to be reduced to original 1/2～2/3, sludge dewatering effect is improved, and moisture is easily isolated with solid, surely
Qualitative enhancing, without obvious stench, toxic bacteria is reduced.The anaerobic digestion of sludge is the main ring for making sludge realize " four change "
Section, first, Organic substance is anaerobically digested decompositions, can make sludge stabilizing, is allowed to be difficult corruption, next, by anaerobic digestion, greatly
Fraction of pathogens bacterium or cricket worm's ovum are killed or are decomposed as Organic substance, make sewage sludge harmlessness, and the 3rd, as sludge is stablized
Change, the biogas of a large amount of high heating values will be produced, as utilization of energy, make recycling sludge, finally, sludge Jing after digestion, wherein
Part organic nitrogen be converted into ammonia nitrogen, improve the fertilizer efficiency of sludge.
In the anaerobic digestion process of sludge, although mainly by concentration and dehydration, but Organic substance is by anaerobic digestion, changes into natural pond
Gas, this is also a kind of digestion process in itself, and in prior art, sludge produces the process of biogas and relies primarily on methanogen to first
Acid, the consumption of acetic acid and methanol, and organic acidic material by enzymes such as cellulase, protease and lipases to Organic substance
Digest and decompose generate after, remove formic acid, acetic acid and the methanol that can directly be utilized by methanogen, the thing of other acid alcohol classes
Matter, such as propanoic acid and butanoic acid etc. cannot function as the substrate that methanogen produces methane, it is impossible to decomposed by methanogen and utilized, very
The wasting of resources is caused in big degree, causes methanogenic efficiency to substantially reduce.
The content of the invention
It is an object of the invention to provide the processing method and its microbial bacterial agent of a kind of anaerobic sludge digestion.
1st, from sewage treatment plant residual sludge, its total solid content of adjustment that adds water is for 5-10%；
2nd, the sludge by solid content obtained by the first step for 5-10% is added in anaerobic digestor, and addition is digestion tank volume
60% (v/v), adds the methane phase inoculum of digestion tank volume 8-10% (v/v), adds water to the 90% of digestion tank volume, adjusts
PH to 7.5-7.7；
The methane phase inoculum is selected from biogas fermentative liquid, manure pit sediment or the anaerobic activated sludge from methane-generating pit；
3rd, inoculating complex microorganism microbial inoculum, addition are the 0.5%-1.2% (v/v) for digesting tank volume, are stirred, and are covered；
4th, keep reaction temperature at 25-35 DEG C or so, stir once per 10h, while stirring, add nitric acid rare earth, react 36h；
The addition of the nitric acid rare earth is by volatile solid in every gram of sludge（VSS）Add 3.0-4.5 μ g nitric acid rare earths.
The nitric acid rare earth can select Lanthanum (III) nitrate (La (NO3)3·6H2O), cerous nitrate (Ce (NO3)3·6H2O)。
The complex micro organism fungicide includes mixed bacteria liquid and carrier, and the mixed bacteria liquid is by Bacillus pumilus, cloaca
It is prepared by enterobacteria, denitrifying bacterium, Paracoccus denitrificans, bacillus amyloliquefaciens, lactococcus lactis.
The Bacillus pumilus are (Bacillus pumilus) ATCC27142；
The enterobacter cloacae is（Enterobacter cloacae）ATCC 700323；
The denitrifying bacterium is（Paracoccus pantotrophus）ATCC 35512；
The Paracoccus denitrificans is (Paracoccus denitrificans) ATCC13543；
The bacillus amyloliquefaciens are（Bacillus amyloliquefaciens）ATCC 23843；
The lactococcus lactis are（Lactococcus lactis）ATCC 11454.
The carrier can be solid carrier and/or liquid-carrier.The solid carrier be mineral material, biomaterial and/
Or macromolecular compound；The mineral material can be clay, Talcum, Kaolin, montmorillonite, white carbon, zeolite, Silicon stone, turfy soil
With at least one in kieselguhr；The biomaterial is the straw of all kinds of crops, pine shell, Caulis et Folium Oryzae, Pericarppium arachidis hypogaeae, Semen Maydis powder, bean
At least one in the feces of powder, starch, turf and animal；The liquid-carrier can for organic solvent, vegetable oil, mineral oil or
Water；The organic solvent is decane and/or dodecane.
The application selects carrier to be made up of following raw materials：Kaolin, shitosan.
Preferably, the carrier compares 1 according to weight by Kaolin, shitosan：3 mix；
The carrier can ensure that microbial inoculum be used for biologic packing material in the good advantage of tack, also with wide material sources, it is with low cost,
Easy to use, preservation term length, the advantages of preparation method is simple.
The complex micro organism fungicide is mixed bacteria liquid and carrier according to 1：2 weight ratio is mixed with biological preparation；
The preparation method of the mixed bacteria liquid is as follows：Bacillus pumilus, enterobacter cloacae, denitrifying bacterium, Paracoccus denitrificans, solution
It is 1 × 10 that bacillus amyloliquefacienses, lactococcus lactis are cultivated respectively to concentration8The bacterium solution of individual/ml, then according to 3:2:2:1:4:3
Volume ratio mixing, obtain final product；
Strain of the present invention can be from American Type Culture collection warehousing（ATCC）It is commercially available.
Those skilled in the art can select suitable culture medium and amplification culture method according to general knowledge, reach viable count
108Individual/gram, and prepare according to the conventional method for preparing microbial solid preparation.
The composite bacteria agent capable of the present invention can fast breeding, so as to reduce initial cost, strong adaptability various will can form excellent
The strain of gesture flora, is configured to high-efficiency microorganism preparation, is added in sludge treating system by a certain amount of, accelerates microorganism to dirt
The degraded of mud, to improve the biological treatment efficiency of system, it is ensured that system stable operation.Close between each strain in microorganism formulation
Reason compatibility, symbiosis are coordinated, mutually not antagonism, and its preparation method is easy, and easy to implement the method, which is easy to operate, beneficial to industrialized production；This
The process operations method of invention is simple, reduces the wasting of resources, and less input, reduces processing cost.
The present invention takes full advantage of bioanalysises and chemical method by adding lifting of the nitric acid rare earth realization to denitrification rate
Advantage so that the denitrification rate of denitrifying bacterium reaches 2mgNO3 -More than-N/ (mg VSSd), it is achieved thereby that to anti-nitre
Change the lifting of bacterium nitrogen removal performance, denitrification methane phase while realizing anaerobic system.
In microbial inoculum of the present invention, Bacillus pumilus and bacillus amyloliquefaciens can produce a large amount of fibers in incubation
Plain enzyme, amylase, the enzyme of generation effectively can carry out decomposition digestion to the Organic substance in sludge；Lactococcus lactis its metabolism is produced
Thing is lactic acid and acetic acid, and the organic acid such as propanoic acid that can cannot be utilized Organic substance after enzyme decomposition by methanogen is converted into second
Acid, so that methanogen can be utilized, produces methane gas, can greatly improve equal volume sludge and produce methane
Amount, improves efficiency well, increases sludge utilization rate, mutually cooperate between each strain, creates suitable oxidoreduction bar
Part, and noxious substance is removed, feedback suppression is released, advantage thermodynamically is created, and sludge is made using more resource.
Specific embodiment
Embodiment 1
Prepare complex microorganism preparations, the complex micro organism fungicide is made up of mixed bacteria liquid and carrier, the mixed bacteria liquid by
It is prepared by Bacillus pumilus, enterobacter cloacae, denitrifying bacterium, Paracoccus denitrificans, bacillus amyloliquefaciens, lactococcus lactis.
The Bacillus pumilus are (Bacillus pumilus) ATCC27142；
The enterobacter cloacae is（Enterobacter cloacae）ATCC 700323；
The denitrifying bacterium is（Paracoccus pantotrophus）ATCC 35512；
The Paracoccus denitrificans is (Paracoccus denitrificans) ATCC13543；
The bacillus amyloliquefaciens are（Bacillus amyloliquefaciens）ATCC 23843；
The lactococcus lactis are（Lactococcus lactis）ATCC 11454.
The carrier compares 1 according to weight by Kaolin, shitosan：3 mix；
The complex micro organism fungicide is mixed bacteria liquid and carrier according to 1：2 weight ratio is mixed with biological preparation；
The preparation method of the mixed bacteria liquid is as follows：Bacillus pumilus, enterobacter cloacae, denitrifying bacterium, Paracoccus denitrificans, solution
It is 1 × 10 that bacillus amyloliquefacienses, lactococcus lactis are cultivated respectively to concentration8The bacterium solution of individual/ml, then according to 3:2:2:1:4:3
Volume ratio mixing, obtain final product；
Strain of the present invention can be from American Type Culture collection warehousing（ATCC）It is commercially available.
Those skilled in the art can select suitable culture medium and amplification culture method according to general knowledge, reach viable count
108Individual/gram, and prepare according to the conventional method for preparing microbial solid preparation.
Embodiment 2
Sludge sample is processed as steps described below from certain sewage treatment plant
1st, it is 8% to adjust sludge and make its solid content；
2nd, the sludge that solid content obtained by the first step is 8% is added in anaerobic digestor, addition is digest tank volume 60%
(v/v), add digestion tank volume 8% (v/v) methane phase inoculum, add water to digestion tank volume 90%, adjust pH to
7.5-7.7；
The methane phase inoculum is biogas fermentative liquid；
3rd, inoculating complex microorganism microbial inoculum, addition are 1% (v/v) for digesting tank volume, are stirred, and are covered；
4th, keep reaction temperature at 30 DEG C, stir once per 10h, while stirring, add nitric acid rare earth, react 36h；
The addition of the nitric acid rare earth is by volatile solid in every gram of sludge（VSS）3 μ g nitric acid rare earths are added, is added altogether
3 times.
The nitric acid rare earth is cerous nitrate (Ce (NO3)3·6H2O)；
The preparation that the microorganism formulation is prepared using embodiment 1.
Treatment effect such as table 1：
1 Treatment of Sludge effect of table
The impact that 3 microbial inoculum composition of embodiment is tested to methane increment
First from 200kg sludge of sewage treatment plant is taken, five parts are divided into, per part is 40kg.
Five parts of sludge of decile are respectively placed in five identical closed reaction vessels, experimental group is respectively labeled as, it is right
According to 1 group, 2 groups are compareed, compare 3 groups, blank control group.
Experimental group：Microbial bacterial agent prepared by Example one, according to the weight ratio of sludge 1%, is seeded to the anti-of experimental group
In answering container, after maintaining anaerobic environment digestion reaction 12h, Methane production in reaction vessel is surveyed using methane measuring instrument
Amount；
Compare 1 group：Microbial bacterial agent in embodiment 1 is removed into denitrifying bacterium, remaining all same is added into reaction vessel, tieed up
After holding anaerobic environment digestion reaction 12h, Methane production in reaction vessel is measured using methane measuring instrument；
Compare 2 groups：Microbial bacterial agent in embodiment 1 is removed into enterobacter cloacae and Paracoccus denitrificans, remaining all same, according to dirt
The weight ratio of mud 1%, adds into reaction vessel, after maintaining anaerobic environment digestion reaction 12h, using methane measuring instrument to reaction
In container, Methane production is measured；
Compare 3 groups：Microbial bacterial agent in embodiment 1 is removed into bacillus amyloliquefaciens, lactococcus lactis bacterium, remaining all same,
According to the weight ratio of sludge 1%, add into reaction vessel, after maintaining anaerobic environment digestion reaction 12h, using methane measuring instrument
Methane production in reaction vessel is measured；
Blank control group adds equivalent water.
Measurement result is as shown in table 2：
2 Methane production of table is determined
| Experimental group | Compare 1 group | Compare 2 groups | Compare 3 groups | Blank control group | |
| The initial aerogenesis time | 2h | 6h | 7h | 4.5h | 12h |
| Methane content m3 | 38.7 | 17.5 | 15.3 | 19.5 | 3.5 |
In experimental group, methane content is much larger than matched group.
Although above having made detailed explanation to this case with general explanation and specific embodiment, in the present invention
On the basis of, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore, not
The modification made on the basis of deviateing present invention spirit or improvement, belong to the scope of protection of present invention.
Claims (9)
1. a kind of processing method of anaerobic sludge digestion, it is characterised in that comprise the steps
（1）It is 5-10wt% that adjustment sludge makes its solid content；
（2）Sludge of the solid content for 5-10wt% is added in anaerobic digestor, addition is the 60% (v/ for digesting tank volume
V), add digestion tank volume 8-10% (v/v) methane phase inoculum, add water to digestion tank volume 90%, adjust pH to
7.5-7.7；
（3）Inoculating complex microorganism microbial inoculum, addition are the 0.5%-1.2% (v/v) for digesting tank volume, are stirred, and are covered；
（4）Keep reaction temperature at 25-35 DEG C or so, stir once per 10h, while stirring, add nitric acid rare earth, react
36h。
2. method according to claim 1, the methane phase inoculum is selected from biogas fermentative liquid, manure pit sediment or is derived from methane-generating pit
Anaerobic activated sludge.
3. the method according to claim 1-2, the addition of the nitric acid rare earth is by volatile solid in every gram of sludge
（VSS）Add 3.0-4.5 μ g nitric acid rare earths.
4. the method according to claim 1-3, the nitric acid rare earth are Lanthanum (III) nitrate or cerous nitrate.
5. the method according to claim 1-4, the complex micro organism fungicide are made up of mixed bacteria liquid and carrier, described mixed
Bacterium solution is closed by Bacillus pumilus, enterobacter cloacae, denitrifying bacterium, Paracoccus denitrificans, bacillus amyloliquefaciens, lactococcus lactis
Prepare.
6. the method according to claim 1-5, it is characterised in that：
The Bacillus pumilus are (Bacillus pumilus) ATCC 27142；
The enterobacter cloacae is（Enterobacter cloacae）ATCC 700323；
The denitrifying bacterium is（Paracoccus pantotrophus）ATCC 35512；
The Paracoccus denitrificans is (Paracoccus denitrificans) ATCC 13543；
The bacillus amyloliquefaciens are（Bacillus amyloliquefaciens）ATCC 23843；
The lactococcus lactis are（Lactococcus lactis）ATCC 11454.
7. the method according to claim 1-6, the carrier compare 1 according to weight by Kaolin, shitosan：3 mix,
The complex micro organism fungicide is mixed bacteria liquid and carrier according to 1：2 weight ratio is mixed with biological preparation.
8. the method according to claim 1-7, it is characterised in that the preparation method of the mixed bacteria liquid is as follows：Short and small bud
Spore bacillus, enterobacter cloacae, denitrifying bacterium, Paracoccus denitrificans, bacillus amyloliquefaciens, lactococcus lactis are cultivated respectively to concentration
For 1 × 108The bacterium solution of individual/ml, then according to 3:2:2:1:4:3 volume ratio mixing, obtains final product.
9. claim 1-8 methods described is used for the purposes that sludge anaerobic is processed.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754543A (en) * | 2017-01-11 | 2017-05-31 | 王筠涵 | A kind of microorganism formulation for sludge anaerobic fermentation |
| CN106906170A (en) * | 2017-05-03 | 2017-06-30 | 上田环境修复股份有限公司 | Complex micro organism fungicide and its preparation method and application |
| CN107555747A (en) * | 2017-10-24 | 2018-01-09 | 赵灿根 | A kind of environment-friendly treatment method that city sewage and sludge is carried out using microorganism formulation |
| CN107601802A (en) * | 2017-10-24 | 2018-01-19 | 赵灿根 | A kind of microbiological garbage treatment agent of sludge anaerobic fermentation |
| CN108706848A (en) * | 2018-05-04 | 2018-10-26 | 上海交通大学 | A kind of method that biology conditioning improves dewatering performance of sludge |
| CN113817781A (en) * | 2021-09-30 | 2021-12-21 | 内蒙古科技大学 | Rare earth additive and preparation method thereof |
| CN115502188A (en) * | 2022-10-19 | 2022-12-23 | 北京时代桃源环境科技股份有限公司 | Exogenous additive and application thereof in anaerobic digestion of kitchen waste |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101864362A (en) * | 2010-05-19 | 2010-10-20 | 南京加德绿色能源研发有限公司 | Compound microbial bacterial preparation and application thereof |
| CN104150727B (en) * | 2014-08-01 | 2015-09-23 | 石家庄开发区德赛化工有限公司 | Utilize the pretreatment process of complex micro organism fungicide reinforcement sludge anaerobic digestion |
| CN105669286A (en) * | 2015-12-30 | 2016-06-15 | 广西农垦明阳生化集团股份有限公司 | Method for preparing organic compound fertilizer through sludge generated by processing cassava starch |
| CN105753272A (en) * | 2016-05-14 | 2016-07-13 | 王浩丞 | Sewage treatment method for garden planning |
-
2016
- 2016-12-29 CN CN201611243920.1A patent/CN106587559A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101864362A (en) * | 2010-05-19 | 2010-10-20 | 南京加德绿色能源研发有限公司 | Compound microbial bacterial preparation and application thereof |
| CN104150727B (en) * | 2014-08-01 | 2015-09-23 | 石家庄开发区德赛化工有限公司 | Utilize the pretreatment process of complex micro organism fungicide reinforcement sludge anaerobic digestion |
| CN105669286A (en) * | 2015-12-30 | 2016-06-15 | 广西农垦明阳生化集团股份有限公司 | Method for preparing organic compound fertilizer through sludge generated by processing cassava starch |
| CN105753272A (en) * | 2016-05-14 | 2016-07-13 | 王浩丞 | Sewage treatment method for garden planning |
Non-Patent Citations (2)
| Title |
|---|
| 夏青等: "镧、铈对厌氧颗粒污泥产甲烷的Hormesis效应及其动力学研究", 《环境科学学报》 * |
| 朱杰编著: "《肉类加工废水生物脱氮工艺过程研究》", 30 June 2007, 西南交通大学出版社 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754543A (en) * | 2017-01-11 | 2017-05-31 | 王筠涵 | A kind of microorganism formulation for sludge anaerobic fermentation |
| CN106906170A (en) * | 2017-05-03 | 2017-06-30 | 上田环境修复股份有限公司 | Complex micro organism fungicide and its preparation method and application |
| CN107555747A (en) * | 2017-10-24 | 2018-01-09 | 赵灿根 | A kind of environment-friendly treatment method that city sewage and sludge is carried out using microorganism formulation |
| CN107601802A (en) * | 2017-10-24 | 2018-01-19 | 赵灿根 | A kind of microbiological garbage treatment agent of sludge anaerobic fermentation |
| CN107601802B (en) * | 2017-10-24 | 2020-12-18 | 杭州富阳飞博科技有限公司 | Microbial treatment agent for anaerobic fermentation of sludge |
| CN108706848A (en) * | 2018-05-04 | 2018-10-26 | 上海交通大学 | A kind of method that biology conditioning improves dewatering performance of sludge |
| CN108706848B (en) * | 2018-05-04 | 2021-03-30 | 上海交通大学 | A method for biological conditioning to improve sludge dewatering performance |
| CN113817781A (en) * | 2021-09-30 | 2021-12-21 | 内蒙古科技大学 | Rare earth additive and preparation method thereof |
| CN113817781B (en) * | 2021-09-30 | 2024-03-26 | 内蒙古科技大学 | Rare earth auxiliary agent and preparation method thereof |
| CN115502188A (en) * | 2022-10-19 | 2022-12-23 | 北京时代桃源环境科技股份有限公司 | Exogenous additive and application thereof in anaerobic digestion of kitchen waste |
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