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CN112568395B - Method for improving the texture and color of vacuum freeze-dried reconstituted apple crisps - Google Patents

Method for improving the texture and color of vacuum freeze-dried reconstituted apple crisps Download PDF

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CN112568395B
CN112568395B CN202011403583.4A CN202011403583A CN112568395B CN 112568395 B CN112568395 B CN 112568395B CN 202011403583 A CN202011403583 A CN 202011403583A CN 112568395 B CN112568395 B CN 112568395B
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毕金峰
易建勇
李旋
金鑫
吴昕烨
吕健
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Institute of Food Science and Technology of CAAS
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/09Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks
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    • A23B2/00Preservation of foods or foodstuffs, in general
    • A23B2/70Preservation of foods or foodstuffs, in general by treatment with chemicals
    • A23B2/725Preservation of foods or foodstuffs, in general by treatment with chemicals in the form of liquids or solids
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    • A23BPRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B2/00Preservation of foods or foodstuffs, in general
    • A23B2/90Preservation of foods or foodstuffs, in general by drying or kilning; Subsequent reconstitution
    • A23B2/92Freeze drying
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    • A23L29/20Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/206Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
    • A23L29/231Pectin; Derivatives thereof
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
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    • A23L29/30Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/40Colouring or decolouring of foods
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    • A23V2400/00Lactic or propionic acid bacteria
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    • A23V2400/113Acidophilus
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23V2400/00Lactic or propionic acid bacteria
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    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/165Paracasei
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract

The invention discloses a method for improving the texture and color of vacuum freeze-dried recombined apple crisp chips, which comprises the following steps: step one, cleaning apples, peeling, removing kernels, crushing and pulping to obtain apple pulp; step two, adding low-ester pectin, calcium chloride, p-hydroxybenzoic acid or derivatives thereof and dry apple peel residue powder into the apple pulp, uniformly mixing, and homogenizing to obtain a mixed solution; adding organic acid to adjust the pH value, sterilizing and cooling; adding a fermentation agent, and fermenting to obtain a fermentation liquid; fifthly, pulping in a sterile environment, and filling nitrogen into the fermentation liquor in the pulping process; step six, freezing to obtain apple pulp blocks; step seven, soaking in chitosan water solution, spreading on a tray, and freezing; and step eight, after freezing, carrying out vacuum freeze drying to obtain the recombined apple crisp chips. The method can effectively solve the problems of unstable storage period and low hardness and brittleness of the recombinant apple crisp chip anthocyanin.

Description

改善真空冷冻干燥重组苹果脆片质构和色泽的方法Method for improving the texture and color of vacuum freeze-dried reconstituted apple crisps

技术领域technical field

本发明涉及苹果脆片加工技术领域。更具体地说,本发明涉及一种改善真空冷冻干燥重组苹果脆片质构和色泽的方法。The invention relates to the technical field of apple crisps processing. More specifically, the present invention relates to a method for improving the texture and color of vacuum freeze-dried reconstituted apple crisps.

背景技术Background technique

近年来,真空冷冻果蔬脆片市场发展迅速。因其具有食用方便、营养健康、易于携带等优点,深受消费者青睐。真空冷冻干燥逐渐成为制备苹果脆片的绿色优质干燥方式。In recent years, the market of vacuum-frozen fruit and vegetable chips has developed rapidly. It is favored by consumers because of its advantages of convenient consumption, nutrition and health, and easy portability. Vacuum freeze-drying has gradually become a green and high-quality drying method for preparing apple chips.

苹果是我国第一大水果,红肉苹果富含花青素,是一种高抗氧化性产品。花青素容易受到光照、氧气等外界因素影响发生降解,特别是由于真空冷冻干燥红肉苹果脆片具有酥松的多孔网络结构,比表面积大,这使得氧气、水分和光线更容易接触花青素,因花青素降解而引起的色泽褪变和功能性减弱成为富含花青素果蔬脆片加工和贮藏过程中难以解决的品质缺陷。长期以来,如何提高果蔬类食品中花青素的稳定性困扰着食品产业,特别是果蔬脆片加工产业。Apple is the largest fruit in my country. Red-fleshed apples are rich in anthocyanins and are a high antioxidant product. Anthocyanins are easily degraded by external factors such as light and oxygen, especially because the vacuum freeze-dried red meat apple crisps have a crisp porous network structure and a large specific surface area, which makes it easier for oxygen, moisture and light to contact anthocyanins , the color fading and functional weakening caused by the degradation of anthocyanins become the quality defects that are difficult to solve during the processing and storage of anthocyanin-rich fruit and vegetable chips. For a long time, how to improve the stability of anthocyanins in fruit and vegetable foods has plagued the food industry, especially the fruit and vegetable chips processing industry.

另一方面,真空冷冻干燥天然果蔬脆片存在硬脆度较低、口感质构绵软的问题。究其原因一是果蔬原料自身组织结构的不具均一性,二是果蔬细胞壁干物质含量较少,干燥后难以支撑果蔬脆片多孔网络结构的刚性。On the other hand, vacuum freeze-dried natural fruit and vegetable chips have the problems of low hardness and brittleness and soft texture. One of the reasons is the inhomogeneity of the tissue structure of the fruit and vegetable raw materials, and the second is that the dry matter content of the fruit and vegetable cell wall is less, and it is difficult to support the rigidity of the porous network structure of the fruit and vegetable chips after drying.

发明内容SUMMARY OF THE INVENTION

本发明的一个目的是解决至少上述问题,并提供至少后面将说明的优点。An object of the present invention is to solve at least the above-mentioned problems and to provide at least the advantages which will be explained later.

本发明还有一个目的是提供一种改善真空冷冻干燥重组苹果脆片质构和色泽的方法,巧妙地利用食品天然化合物之间的自发化学互作机制,设计了基于细胞壁果胶多糖、对羟基苯甲酸(4-羟基苯甲酸或其衍生物)和花青素三者的基于大小分子相互作用的花青素自发稳态化工艺,同时利用质构重组、充气打浆、冻融处理等物理手段调控冰晶大小和产品孔隙特征,创制了一种全新的重组红肉苹果脆片,有效解决了产品花青素贮藏期不稳定,产品硬脆度不高的问题。Another object of the present invention is to provide a method for improving the texture and color of reconstituted apple crisps by vacuum freeze-drying, cleverly utilizing the spontaneous chemical interaction mechanism between natural food compounds, and designing a method based on cell wall pectin polysaccharide, p-hydroxyl Spontaneous stabilization process of anthocyanins based on the interaction of large and small molecules between benzoic acid (4-hydroxybenzoic acid or its derivatives) and anthocyanins, and at the same time using physical means such as texture reorganization, aeration beating, freeze-thaw treatment, etc. By adjusting the size of ice crystals and the pore characteristics of the product, a brand-new reconstituted red-meat apple crisp was created, which effectively solved the problems of unstable storage period of anthocyanins and low hardness and brittleness of the product.

为了实现根据本发明的这些目的和其它优点,提供了一种改善真空冷冻干燥重组苹果脆片质构和色泽的方法,包括以下步骤:In order to achieve these objects and other advantages according to the present invention, a method for improving the texture and color of vacuum freeze-dried reconstituted apple crisps is provided, comprising the following steps:

步骤一、将苹果洗净、去皮、去核、破碎、打浆,得苹果浆;Step 1. Wash, peel, core, crush and beat the apples to obtain apple pulp;

步骤二、向苹果浆中按10~50g/kg添加低酯果胶、1~2mg/g添加氯化钙、0.012-0.5g/kg添加对羟基苯甲酸或其衍生物、添加质量分数2~5%的苹果皮渣干粉,混合均匀后均质,得混合液;Step 2: Add low-ester pectin at 10-50 g/kg, add calcium chloride at 1-2 mg/g, add p-hydroxybenzoic acid or its derivative at 0.012-0.5 g/kg, and add a mass fraction of 2- 5% dry powder of apple peel residue, mixed evenly and homogenized to obtain a mixed solution;

步骤三、加入有机酸调节步骤二中的混合液的pH至3.0~3.8,杀菌、冷却;Step 3, adding organic acid to adjust the pH of the mixed solution in step 2 to 3.0-3.8, sterilizing and cooling;

步骤四,按10~20g/kg加入发酵剂,于30℃条件下发酵48h,得发酵液,其中,发酵剂为复合乳酸菌和植物乳杆菌数量按2:1混合得到;Step 4, adding a starter at 10-20g/kg, and fermenting at 30°C for 48h to obtain a fermented liquid, wherein the starter is obtained by mixing compound lactic acid bacteria and Lactobacillus plantarum at a ratio of 2:1;

步骤五、置于无菌环境中打浆2~5min,打浆过程中向发酵液中充入氮气;Step 5, beating in a sterile environment for 2 to 5 minutes, and filling the fermentation broth with nitrogen during the beating process;

步骤六、置于-18℃下冷冻,得苹果浆块,其中,苹果浆块的厚度为3~8mm;Step 6, freezing at -18°C to obtain apple pulp blocks, wherein the thickness of the apple pulp blocks is 3-8 mm;

步骤七、置于-1~0℃的质量分数为30~50%的壳聚糖水溶液浸泡2~5s,摊平到-40℃的托盘上,置于-18℃条件下冷冻;Step 7. Immerse the chitosan aqueous solution with a mass fraction of 30 to 50% at -1 to 0 °C for 2 to 5 s, spread it on a tray at -40 °C, and freeze at -18 °C;

步骤八、冷冻好后,真空冷冻干燥,得苹果脆片;Step 8. After freezing, vacuum freeze-drying to obtain apple crisps;

其中,步骤二和步骤四的添加量均以苹果浆的质量为基准。Wherein, the addition amount of step 2 and step 4 is based on the quality of apple pulp.

优选的是,对羟基苯甲酸衍生物包括:对羟基苯甲酸乙酯、羟基苯甲酸乙酯钠、对羟基苯甲酸丙酯、对羟基苯甲酸甲酯钠的任意一种或几种的组合。Preferably, the p-hydroxybenzoic acid derivatives include: any one or a combination of ethyl p-hydroxybenzoate, sodium ethyl p-hydroxybenzoate, propyl p-hydroxybenzoate and sodium methyl p-hydroxybenzoate.

优选的是,步骤三中杀菌的温度为121℃,杀菌时间为15min。Preferably, the temperature of the sterilization in step 3 is 121° C., and the sterilization time is 15 minutes.

优选的是,发酵剂的菌浓度为8.0~9.0Log(CFU/ml)。Preferably, the bacterial concentration of the starter is 8.0 to 9.0 Log (CFU/ml).

优选的是,步骤六中冷冻时采用模具托盘容纳苹果浆。Preferably, a mold tray is used to hold the apple pulp during freezing in step 6.

优选的是,将步骤八中的苹果脆片进行避光隔氧包装。Preferably, the apple crisps in step 8 are packaged in a light and oxygen barrier.

优选的是,步骤一中的破碎的方法为:置于低温充氮破碎机中破碎。Preferably, the crushing method in step 1 is: placing in a low-temperature nitrogen-filled crusher and crushing.

优选的是,苹果的品种为红肉苹果。Preferably, the apple variety is a red-fleshed apple.

优选的是,有机酸为柠檬酸、苹果酸、乳酸的一种或几种的组合。Preferably, the organic acid is one or a combination of citric acid, malic acid and lactic acid.

优选的是,复合乳酸菌为长双歧杆菌、嗜酸乳杆菌、发酵乳杆菌、瑞士乳杆菌、副干酪乳杆菌、鼠李糖乳杆菌、嗜热链球菌的一种或几种的组合。Preferably, the compound lactic acid bacteria is one or a combination of Bifidobacterium longum, Lactobacillus acidophilus, Lactobacillus fermentum, Lactobacillus helveticus, Lactobacillus paracasei, Lactobacillus rhamnosus, and Streptococcus thermophilus.

本发明至少包括以下有益效果:The present invention includes at least the following beneficial effects:

第一、巧妙地利用食品天然化合物之间的自发化学互作机制,设计了基于细胞壁果胶多糖、对羟基苯甲酸(4-羟基苯甲酸或其衍生物)和花青素三者的基于大小分子相互作用的花青素自发稳态化工艺,同时利用质构重组、充气打浆、冻融处理等物理手段调控冰晶大小和产品孔隙特征,创制了一种全新的重组红肉苹果脆片,有效解决了产品花青素贮藏期不稳定,产品硬脆度不高的问题。First, by cleverly exploiting the spontaneous chemical interaction mechanism between food natural compounds, a size-based size-based cell wall pectin polysaccharide, p-hydroxybenzoic acid (4-hydroxybenzoic acid or its derivatives) and anthocyanin were designed. The spontaneous stabilization process of anthocyanins through molecular interaction, and at the same time using physical means such as texture reorganization, aeration beating, freeze-thaw treatment and other physical means to control the ice crystal size and product pore characteristics, has created a brand-new reconstituted red meat apple crisp, which is effective The problem of unstable storage period of anthocyanins and low hardness and brittleness of products is solved.

第二、通过精心设计基于食品大小分子相互作用的稳态化机制,加上物理氧气阻隔技术,提高了产品贮藏期间花青素的稳定性。具体来说,该方案利用低甲酯化度果胶分子上丰富的羧基在其等电点以上带负电,花青素在等电点以下带正电,由此发生以静电作用为主,辅以氢键和疏水作用的相互结合;与此同时,利用小分子对羟基苯甲酸苯环与花青素苯环发生非共价键“Π-Π重叠”,从两个维度稳定了限制花青素的化学结构,使得B环受到空间位阻不能自由活动,限制了花青素化学键的解离,大幅提高了花青素的化学稳定性;针对红肉苹果中主要呈色物质矢车菊素-3-葡萄糖苷的保护效果,本发明同时也尝试了黄酮和阿魏酸的护色效果,均不如对羟基苯甲酸或其衍生物效果明显。Second, the stability of anthocyanins during product storage has been improved through careful design of the homeostasis mechanism based on the interaction of large and small molecules in food, coupled with physical oxygen barrier technology. Specifically, this scheme utilizes the abundant carboxyl groups on the pectin molecule with low methyl esterification degree to be negatively charged above its isoelectric point, and the anthocyanin to be positively charged below the isoelectric point, which is mainly caused by electrostatic interaction and secondary At the same time, the non-covalent bond "Π-Π overlap" between the small molecule p-hydroxybenzoic acid benzene ring and the anthocyanin benzene ring is used to stabilize the restricted cyanine from two dimensions. The chemical structure of anthocyanin makes the B ring unable to move freely due to steric hindrance, which limits the dissociation of anthocyanin chemical bonds and greatly improves the chemical stability of anthocyanin. The protection effect of -3-glucoside, the present invention also tried the color protection effect of flavonoids and ferulic acid, both of which are not as obvious as p-hydroxybenzoic acid or its derivatives.

第三、通过壳聚糖涂膜处理,在苹果脆片外围形成了一层果胶覆膜,该层涂膜可以有效阻隔氧气、水分的渗入,维持了花青素的低氧、低水分活度环境,进一步抑制花青素的降解,这也有效贮藏期间花青素的降解问题。Third, through the chitosan coating treatment, a layer of pectin coating is formed on the periphery of the apple chips, which can effectively block the infiltration of oxygen and water, and maintain the low oxygen and low water activity of anthocyanins. The temperature environment further inhibits the degradation of anthocyanins, which is also effective in the degradation of anthocyanins during storage.

第四、壳聚糖涂膜还可降低了苹果脆片的吸湿速率,缓解了真空冷冻苹果脆片开袋后因快速吸潮而导致的酥脆性丧失问题,对于维持产品酥脆的质构有明显效果。Fourth, the chitosan coating can also reduce the moisture absorption rate of apple crisps, alleviate the problem of the loss of crispness caused by rapid moisture absorption after the vacuum-frozen apple crisps are opened, and maintain the crisp texture of the product. Effect.

第五、采用充气打浆工艺处理果浆原料,使冻结后果块中保留一定比例的气泡,这些气泡再真空冷冻干燥初始因真空度提高将迅速膨胀并溢出,留下的孔道,这些孔道在提高干燥速率的同时;更重要的,这种工艺可精准调控冻干苹果脆片的多孔网络结构,形成具有更大孔隙和更厚孔隙壁的疏松孔隙结构,由此获得更佳酥脆的口感。Fifth, use the aeration beating process to treat the pulp raw materials, so that a certain proportion of air bubbles remain in the frozen block. These air bubbles will expand and overflow rapidly due to the increase of vacuum degree at the beginning of vacuum freeze-drying. More importantly, this process can precisely control the porous network structure of freeze-dried apple chips to form a loose pore structure with larger pores and thicker pore walls, thereby obtaining a better crispy taste.

第六、红肉苹果含糖量高达13%左右,导致其共融点非常低,由此需要降低冻干时的物料温度才能确保产品维持固态结构而不塌陷,这导致苹果的冻干时间和能耗都显著高于其它低糖原料;本发明一方面通过发酵过程降低了苹果浆中小分子糖的含量,一方面还通过加入苹果皮渣提高产品的高分子量膳食纤维。小分子糖的共融点和玻璃化转变温度低,高分子膳食纤维共融点和玻璃化转变温度高,基于上述正反两方面的作用,大幅提高了苹果混合浆料的共融点,由此可以使用更高的冻干温度,显著提高了升华速率,缩短了干燥时间。Sixth, the sugar content of red-fleshed apples is as high as about 13%, resulting in a very low melting point. Therefore, it is necessary to reduce the material temperature during freeze-drying to ensure that the product maintains a solid structure and does not collapse, which leads to the freeze-drying time and energy of apples. The consumption is significantly higher than other low-sugar raw materials; on the one hand, the present invention reduces the content of small molecular sugar in the apple pulp through the fermentation process, and on the other hand, the high molecular weight dietary fiber of the product is increased by adding apple peel residue. The melting point and glass transition temperature of small molecular sugar are low, and the melting point and glass transition temperature of macromolecular dietary fiber are high. Based on the above-mentioned positive and negative effects, the eutectic point of apple mixed slurry is greatly improved, so it can be used Higher freeze-drying temperature significantly increases the sublimation rate and shortens the drying time.

本发明的其它优点、目标和特征将部分通过下面的说明体现,部分还将通过对本发明的研究和实践而为本领域的技术人员所理解。Other advantages, objects, and features of the present invention will appear in part from the description that follows, and in part will be appreciated by those skilled in the art from the study and practice of the invention.

附图说明Description of drawings

图1为本发明的各实施例和对比例制备的产品外观图。Fig. 1 is the appearance diagram of the product prepared by each embodiment of the present invention and the comparative example.

具体实施方式Detailed ways

下面结合附图对本发明做进一步的详细说明,以令本领域技术人员参照说明书文字能够据以实施。The present invention will be further described in detail below with reference to the accompanying drawings, so that those skilled in the art can implement it with reference to the description.

需要说明的是,下述实施方案中所述实验方法,如无特殊说明,均为常规方法,所述试剂和材料,如无特殊说明,均可从商业途径获得;在本发明的描述中,术语指示的方位或位置关系为基于附图所示的方位或位置关系,仅是为了便于描述本发明和简化描述,并不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作,因此不能理解为对本发明的限制。It should be noted that the experimental methods described in the following embodiments are conventional methods unless otherwise specified, and the reagents and materials can be obtained from commercial sources unless otherwise specified; in the description of the present invention, The orientation or positional relationship indicated by the terms is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the referred device or element must have a specific orientation or a specific orientation. The orientation configuration and operation are therefore not to be construed as limitations of the present invention.

<实施例1><Example 1>

改善真空冷冻干燥重组苹果脆片质构和色泽的方法,包括以下步骤:A method for improving the texture and color of vacuum freeze-dried reconstituted apple crisps, comprising the following steps:

预处理:选取成熟度均一、无病虫害的红肉苹果,将苹果清洗干净后去皮、去核,纵切成两半;Pretreatment: Select red-fleshed apples with uniform maturity and no pests and diseases, clean the apples, peel and core, and cut them in half lengthwise;

破碎:将苹果置于低温充氮破碎机中破碎;Crushing: The apples are crushed in a low-temperature nitrogen-filled crusher;

打浆:将苹果置于打浆机中打浆,得苹果浆;Beating: put apples in a beater and beat to get apple pulp;

调配:向苹果浆中按50g/kg添加低酯果胶、1.5mg/g添加氯化钙、0.25g/kg添加对羟基苯甲酸乙酯、添加质量分数3%的苹果皮渣干粉,混合均匀后均质,得混合液;添加量均以苹果浆的质量为基准;Preparation: add low-ester pectin, 1.5mg/g calcium chloride, 0.25g/kg ethylparaben, and 3% dry powder of apple peel residue to apple pulp at a rate of 50g/kg, mix well After homogenization, a mixed liquid is obtained; the addition amount is based on the quality of apple pulp;

调节pH:加入柠檬酸调节pH至3.5;Adjust pH: add citric acid to adjust pH to 3.5;

灭菌:将苹果浆置于121℃杀菌15min,冷却后倒入发酵罐;Sterilization: Sterilize the apple pulp at 121°C for 15min, and pour it into the fermentation tank after cooling;

发酵:向发酵罐中的按15g/kg加入发酵剂,发酵剂的菌浓度为8.5Log(CFU/mL),其中复合乳酸菌和植物乳杆菌数量为2:1,于30℃条件下发酵48h,得发酵液;添加量均以苹果浆的质量为基准;Fermentation: add a starter at 15g/kg to the fermenter, the bacterial concentration of the starter is 8.5Log (CFU/mL), wherein the number of compound lactic acid bacteria and Lactobacillus plantarum is 2:1, fermented at 30°C for 48h, The fermentation broth was obtained; the addition amount was based on the quality of apple pulp;

充气打浆:在无菌、充氮环境中打浆3min;打浆过程中容器下方不断混入洁净氮气;Air-filled beating: beating for 3 minutes in a sterile, nitrogen-filled environment; clean nitrogen is continuously mixed under the container during the beating process;

再造型:倒入模具托盘,与模具托盘一起至于-18℃缓慢冻结,得苹果浆块,其中,苹果浆块的厚度为5mm;冻结后的苹果浆块可放在-18℃下长期贮藏,贮藏期可达12个月,可实现终年生产;Remodeling: pour it into the mold tray, and slowly freeze it at -18°C together with the mold tray to obtain apple pulp blocks, wherein the thickness of the apple pulp blocks is 5mm; the frozen apple pulp blocks can be stored at -18°C for a long time. The storage period can be up to 12 months, and the year-round production can be realized;

涂膜:将冻结状态的苹果浆块置于-1℃的质量分数为40%的壳聚糖水溶液中浸泡3s,取出后摊平到-40℃的不锈钢托盘上,并重新放回-18℃低温冻库备用;Coating: Immerse the frozen apple pulp block in a chitosan aqueous solution with a mass fraction of 40% at -1°C for 3s, take it out and flatten it on a stainless steel tray at -40°C, and put it back at -18°C Low temperature freezer for backup;

真空冷冻干燥:将冻结状态苹果浆块转移至真空冷冻干燥仓进行真空冷冻干燥,直至苹果浆块的水分含量为7%以下时,停止真空冷冻干燥,得苹果脆片,其中,设置冷阱温度为-50℃,托盘温度为50℃;Vacuum freeze-drying: transfer the frozen apple pulp block to a vacuum freeze-drying chamber for vacuum freeze-drying until the moisture content of the apple pulp block is below 7%, stop the vacuum freeze-drying, and obtain apple chips, wherein the temperature of the cold trap is set is -50°C, and the tray temperature is 50°C;

充氮包装:将苹果脆片取出,选择避光隔氧包装,进行充氮包装,并将充氮包装好的产品至于阴凉、干燥场所贮藏。Nitrogen-filled packaging: Take out the apple chips, choose a light-proof and oxygen-isolated packaging, carry out nitrogen-filled packaging, and store the nitrogen-filled products in a cool, dry place.

<实施例2><Example 2>

改善真空冷冻干燥重组苹果脆片质构和色泽的方法,包括以下步骤:A method for improving the texture and color of vacuum freeze-dried reconstituted apple crisps, comprising the following steps:

预处理:选取成熟度均一、无病虫害的红肉苹果,将苹果清洗干净后去皮、去核,纵切成两半;Pretreatment: Select red-fleshed apples with uniform maturity and no pests and diseases, clean the apples, peel and core, and cut them in half lengthwise;

破碎:将苹果置于低温充氮破碎机中破碎;Crushing: The apples are crushed in a low-temperature nitrogen-filled crusher;

打浆:将苹果置于打浆机中打浆,得苹果浆;Beating: put apples in a beater and beat to get apple pulp;

调配:向苹果浆中按10g/kg添加低酯果胶、1mg/g添加氯化钙、0.012g/kg添加羟基苯甲酸乙酯钠、添加质量分数2%的苹果皮渣干粉,混合均匀后均质,得混合液;添加量均以苹果浆的质量为基准;Preparation: Add 10g/kg of low-ester pectin, 1mg/g of calcium chloride, 0.012g/kg of sodium ethyl hydroxybenzoate, and 2% dry powder of apple peel residue to apple pulp, and mix them evenly. Homogenize to obtain a mixed solution; the amount of addition is based on the quality of apple pulp;

调节pH:加入苹果酸调节pH至3.0;Adjust pH: add malic acid to adjust pH to 3.0;

灭菌:将苹果浆置于121℃杀菌15min,冷却后倒入发酵罐;Sterilization: Sterilize the apple pulp at 121°C for 15min, and pour it into the fermentation tank after cooling;

发酵:向发酵罐中的按10g/kg加入发酵剂,发酵剂的菌浓度为8.0Log(CFU/mL),其中复合乳酸菌和植物乳杆菌数量为1:1,于30℃条件下发酵36h,得发酵液;添加量均以苹果浆的质量为基准;Fermentation: add a starter at 10g/kg to the fermenter, the bacterial concentration of the starter is 8.0Log (CFU/mL), and the number of compound lactic acid bacteria and Lactobacillus plantarum is 1:1, fermented at 30°C for 36h, The fermentation broth was obtained; the addition amount was based on the quality of apple pulp;

充气打浆:在无菌、充氮环境中打浆2min;打浆过程中容器下方不断混入洁净氮气;Air-filled beating: beating in a sterile, nitrogen-filled environment for 2 minutes; clean nitrogen is continuously mixed under the container during the beating process;

再造型:倒入模具托盘,与模具托盘一起至于-18℃缓慢冻结,得苹果浆块,其中,苹果浆块的厚度为3mm;冻结后的苹果浆块可放在-18℃下长期贮藏,贮藏期可达12个月,可实现终年生产;Remodeling: pour it into the mold tray, and slowly freeze it at -18°C together with the mold tray to obtain apple pulp blocks, of which the thickness of the apple pulp blocks is 3mm; the frozen apple pulp blocks can be stored at -18°C for a long time. The storage period can be up to 12 months, and the year-round production can be realized;

涂膜:将冻结状态的苹果浆块置于-1℃的质量分数为30%的壳聚糖水溶液中浸泡2s,取出后摊平到-30℃的不锈钢托盘上,并重新放回-18℃低温冻库备用;Coating: Immerse the frozen apple pulp block in a chitosan aqueous solution with a mass fraction of 30% at -1°C for 2s, take it out and flatten it on a stainless steel tray at -30°C, and put it back at -18°C Low temperature freezer for backup;

真空冷冻干燥:将冻结状态苹果浆块转移至真空冷冻干燥仓进行真空冷冻干燥,直至苹果浆块的水分含量为7%以下时,停止真空冷冻干燥,得苹果脆片,其中,冷阱温度为-50℃,托盘温度为20℃;Vacuum freeze-drying: transfer the frozen apple pulp block to a vacuum freeze-drying bin for vacuum freeze-drying until the moisture content of the apple pulp block is below 7%, stop the vacuum freeze-drying, and obtain apple chips, wherein the cold trap temperature is -50℃, the tray temperature is 20℃;

充氮包装:将苹果脆片取出,选择避光隔氧包装,进行充氮包装,并将充氮包装好的产品至于阴凉、干燥场所贮藏。Nitrogen-filled packaging: Take out the apple chips, choose a light-proof and oxygen-isolated packaging, carry out nitrogen-filled packaging, and store the nitrogen-filled products in a cool, dry place.

<实施例3><Example 3>

改善真空冷冻干燥重组苹果脆片质构和色泽的方法,包括以下步骤:A method for improving the texture and color of vacuum freeze-dried reconstituted apple crisps, comprising the following steps:

预处理:选取成熟度均一、无病虫害的红肉苹果,将苹果清洗干净后去皮、去核,纵切成两半;Pretreatment: Select red-fleshed apples with uniform maturity and no pests and diseases, clean the apples, peel and core, and cut them in half lengthwise;

破碎:将苹果置于低温充氮破碎机中破碎;Crushing: The apples are crushed in a low-temperature nitrogen-filled crusher;

打浆:将苹果置于打浆机中打浆,得苹果浆;Beating: put apples in a beater and beat to get apple pulp;

调配:向苹果浆中按25g/kg添加低酯果胶、2mg/g添加氯化钙、0.5g/kg添加对羟基苯甲酸丙酯、添加质量分数5%的苹果皮渣干粉,混合均匀后均质,得混合液;添加量均以苹果浆的质量为基准;Preparation: add low-ester pectin at 25g/kg, add calcium chloride at 2mg/g, add propylparaben at 0.5g/kg, add 5% dry powder of apple peel residue to apple pulp, and mix evenly. Homogenize to obtain a mixed solution; the amount of addition is based on the quality of apple pulp;

调节pH:加入乳酸调节pH至3.8;Adjust pH: add lactic acid to adjust pH to 3.8;

灭菌:将苹果浆置于121℃杀菌15min,冷却后倒入发酵罐;Sterilization: Sterilize the apple pulp at 121°C for 15min, and pour it into the fermentation tank after cooling;

发酵:向发酵罐中的按20g/kg加入发酵剂,发酵剂的菌浓度为9.0Log(CFU/mL),其中复合乳酸菌和植物乳杆菌数量为3:1,于37℃条件下发酵60h,得发酵液;添加量均以苹果浆的质量为基准;Fermentation: add a starter at 20g/kg to the fermenter, the bacterial concentration of the starter is 9.0Log (CFU/mL), wherein the number of compound lactic acid bacteria and Lactobacillus plantarum is 3:1, fermented at 37°C for 60h, The fermentation broth was obtained; the addition amount was based on the quality of apple pulp;

充气打浆:在无菌、充氮环境中打浆5min;打浆过程中容器下方不断混入洁净氮气;Air-filled beating: beating for 5 minutes in a sterile, nitrogen-filled environment; clean nitrogen is continuously mixed under the container during the beating process;

再造型:倒入模具托盘,与模具托盘一起至于-18℃缓慢冻结,得苹果浆块,其中,苹果浆块的厚度为8mm;冻结后的苹果浆块可放在-18℃下长期贮藏,贮藏期可达12个月,可实现终年生产;Remodeling: pour it into the mold tray, and slowly freeze it at -18°C together with the mold tray to obtain apple pulp blocks, of which the thickness of the apple pulp blocks is 8mm; the frozen apple pulp blocks can be stored at -18°C for a long time. The storage period can be up to 12 months, and the year-round production can be realized;

涂膜:将冻结状态的苹果浆块置于0℃的质量分数为50%的壳聚糖水溶液中浸泡5s,取出后摊平到-45℃的不锈钢托盘上,并重新放回-18℃低温冻库备用;Coating: Immerse the frozen apple pulp block in a 50% chitosan aqueous solution at 0°C for 5s, take it out and flatten it on a stainless steel tray at -45°C, and put it back to -18°C low temperature Freezer spare;

真空冷冻干燥:将冻结状态苹果浆块转移至真空冷冻干燥仓进行真空冷冻干燥,直至苹果浆块的水分含量为7%以下时,停止真空冷冻干燥,得苹果脆片,其中,冷阱温度为-50℃,托盘温度为80℃;Vacuum freeze-drying: transfer the frozen apple pulp block to a vacuum freeze-drying bin for vacuum freeze-drying until the moisture content of the apple pulp block is below 7%, stop the vacuum freeze-drying, and obtain apple chips, wherein the cold trap temperature is -50℃, the tray temperature is 80℃;

充氮包装:将苹果脆片取出,选择避光隔氧包装,进行充氮包装,并将充氮包装好的产品至于阴凉、干燥场所贮藏。Nitrogen-filled packaging: Take out the apple chips, choose a light-proof and oxygen-isolated packaging, carry out nitrogen-filled packaging, and store the nitrogen-filled products in a cool, dry place.

<实施例4><Example 4>

苹果脆片的制备方法同实施例1,其中,不同的是,调配步骤中采用对羟基苯甲酸甲酯钠代替对羟基苯甲酸乙酯,用量为0.25g/kg。The preparation method of apple crisps is the same as that of Example 1, and the difference is that in the preparation step, sodium methyl parahydroxybenzoate is used instead of ethyl parahydroxybenzoate, and the amount is 0.25 g/kg.

<实施例5><Example 5>

苹果脆片的制备方法同实施例1,其中,不同的是,调配步骤中采用对羟基苯甲酸代替对羟基苯甲酸乙酯,用量为0.25g/kg。The preparation method of apple crisps is the same as that of Example 1, and the difference is that in the preparation step, p-hydroxybenzoic acid is used instead of ethyl p-hydroxybenzoate, and the dosage is 0.25 g/kg.

<对比例1><Comparative Example 1>

苹果脆片的制备方法同实施例1,其中,不同的是,调配步骤中采用黄酮代替对羟基苯甲酸乙酯,用量为0.25g/kg。The preparation method of apple crisps is the same as that of Example 1, and the difference is that flavonoids are used instead of ethyl p-hydroxybenzoate in the preparation step, and the dosage is 0.25 g/kg.

<对比例2><Comparative Example 2>

苹果脆片的制备方法同实施例1,其中,不同的是,调配步骤中采用阿魏酸代替对羟基苯甲酸乙酯,用量为0.25g/kg。The preparation method of apple crisps is the same as that of Example 1, and the difference is that ferulic acid is used instead of ethyl p-hydroxybenzoate in the preparation step, and the dosage is 0.25g/kg.

<对比例3><Comparative Example 3>

苹果脆片的制备方法同实施例1,其中,不同的是,调配步骤中采用水代替对羟基苯甲酸乙酯,用量为0.25g/kg。The preparation method of apple chips is the same as that of Example 1, and the difference is that in the preparation step, water is used instead of ethyl p-hydroxybenzoate, and the amount is 0.25 g/kg.

<对比例4><Comparative Example 4>

苹果脆片的制备方法同实施例1,其中,不同的是,调配步骤中采用水代替低酯果胶,用量为50g/kg。The preparation method of apple crisps is the same as that in Example 1, and the difference is that in the preparation step, water is used instead of low-ester pectin, and the dosage is 50 g/kg.

<对比例5><Comparative Example 5>

苹果脆片的制备方法同实施例1,其中,不同的是,调配步骤中采用高酯果胶代替低酯果胶,用量为50g/kg。The preparation method of apple crisps is the same as that of Example 1, and the difference is that high-ester pectin is used instead of low-ester pectin in the compounding step, and the dosage is 50 g/kg.

<对比例6><Comparative Example 6>

苹果脆片的制备方法同实施例1,其中,不同的是,充气打浆步骤中未采用充气处理,只进行打浆。The preparation method of apple crisps is the same as that in Example 1, except that the aeration treatment is not adopted in the aeration beating step, and only beating is performed.

<对比例7><Comparative Example 7>

苹果脆片的制备方法,包括以下步骤:The preparation method of apple crisps includes the following steps:

预处理:选取成熟度均一、无病虫害的红肉苹果,将苹果清洗干净后去核,纵切成两半;Pretreatment: Select red-fleshed apples with uniform maturity and no pests and diseases, clean the apples, remove the core, and cut them in half lengthwise;

破碎:将苹果置于低温充氮破碎机中破碎;Crushing: The apples are crushed in a low-temperature nitrogen-filled crusher;

打浆:将苹果置于打浆机中打浆,得苹果浆;Beating: put apples in a beater and beat to get apple pulp;

灭菌:将苹果浆置于121℃杀菌15min;Sterilization: Sterilize apple pulp at 121°C for 15min;

真空冷冻干燥:倒入模具托盘,与模具托盘一起至于真空冷冻干燥仓进行真空冷冻干燥,直至苹果浆块的水分含量为7%以下时,停止真空冷冻干燥,得苹果脆片,其中,冷阱温度为-50℃,托盘温度为50℃,苹果浆倒入模具托盘的厚度为5mm;Vacuum freeze-drying: pour into the mold tray, and carry out vacuum freeze-drying in the vacuum freeze-drying chamber together with the mold tray. When the moisture content of the apple pulp block is below 7%, the vacuum freeze-drying is stopped to obtain apple crisps. The temperature is -50°C, the tray temperature is 50°C, and the thickness of the apple pulp into the mold tray is 5mm;

充氮包装:将苹果脆片取出,选择避光隔氧包装,进行充氮包装,并将充氮包装好的产品至于阴凉、干燥场所贮藏。Nitrogen-filled packaging: Take out the apple chips, choose a light-proof and oxygen-isolated packaging, carry out nitrogen-filled packaging, and store the nitrogen-filled products in a cool, dry place.

<苹果脆片品质检测><Quality Inspection of Apple Chips>

1、检测方法1. Detection method

花青素保留率测定:Determination of anthocyanin retention rate:

采用紫外分光光度法测定样品中总花青素含量。使用2%盐酸甲醇溶液提取样品中的花青素,过滤后于530nm条件下测定。通过标准曲线计算总花青素含量。The total anthocyanin content in the samples was determined by UV spectrophotometry. The anthocyanins in the samples were extracted with 2% hydrochloric acid methanol solution, filtered and measured at 530 nm. Total anthocyanin content was calculated by standard curve.

色差值的测定:Determination of color difference value:

采用色彩色差仪测定果蔬脆片的色泽,本实验用△L、△a、△b、△E值代表被测样品的色泽(L、a、b)与鲜样的(L*、a*、b*)的色差值。△E计算方法如下:The color of the fruit and vegetable chips was measured by a colorimeter. In this experiment, the △L, △a, △b, and △E values were used to represent the color (L, a, b) of the tested sample and the fresh sample (L*, a*, b*) color difference value. The calculation method of △E is as follows:

Figure BDA0002817851530000081
Figure BDA0002817851530000081

式中,L、L*分别为鲜果和干燥后脆片的明度值;a、a*分别是鲜果和干燥后脆片的红绿值;b和b*分别是鲜果和干燥后脆片的黄蓝值;ΔL为总色差值。In the formula, L and L* are the brightness values of fresh fruit and dried chips, respectively; a, a* are the red and green values of fresh fruit and dried chips, respectively; b and b* are the yellowness of fresh fruit and dried chips, respectively. Blue value; ΔL is the total color difference value.

共融点测定:Fusion point determination:

采用电阻法测定物料共融点。将果浆置于-40℃条件下完全冻结,然后逐步升高温度。期间使用电阻仪持续检测物料的电阻,当电阻开始呈现急速减小时的温度即为物料的共融点。The material eutectic point was determined by resistance method. Freeze the pulp completely at -40°C, then gradually increase the temperature. During the period, the resistance meter is used to continuously detect the resistance of the material. When the resistance begins to decrease rapidly, the temperature is the eutectic point of the material.

保质期判定:Shelf life determination:

将产品置于25℃条件下贮藏,跟踪产品品质,以产品花青素降解50%以上,或者a值下降20%以上作为保质期终点。Store the product at 25°C, track the quality of the product, and take the product anthocyanin degraded by more than 50% or the a value of more than 20% as the end of the shelf life.

硬脆度的测定:Determination of hardness and brittleness:

用TA-XT2i/50型物性测定仪测定,选取形状、大小相近的脆条进行质构测定。从包装中迅速取出样品后,用物性测定仪做削切试验,重复10次,最后取平均值。脆度用测试产生峰数的多少来表示,单位为“个”。测试数值在一定范围内呈正相关增长,峰数越多,则产品的酥脆度越好,反之则产品的酥脆度越差。Measure with TA-XT2i/50 type physical property analyzer, and select brittle strips with similar shape and size for texture determination. After quickly taking out the sample from the package, use the physical property analyzer to do the cutting test, repeat 10 times, and finally take the average value. The brittleness is expressed by the number of peaks produced by the test, and the unit is "pieces". The test value increases in a positive correlation within a certain range. The more peaks, the better the crispness of the product, and vice versa, the worse the crispness of the product.

孔隙特征分析:Pore Feature Analysis:

采用微-CT扫描进行,采用微-CT自带分析软件分析后获得物料内部平均孔隙直径和孔隙壁厚。The micro-CT scan was used, and the internal average pore diameter and pore wall thickness of the material were obtained after analysis by the micro-CT self-contained analysis software.

益生菌含量测定:Determination of probiotic content:

采用国家标准GB 4789.35-2016食品安全国家标准食品微生物学检验乳酸菌检验。Adopt the national standard GB 4789.35-2016 food safety national standard food microbiology inspection lactic acid bacteria inspection.

2、检测结果2. Test results

实施例1~5和对比例1~7的检测结果分别如表1和表2所示。The detection results of Examples 1 to 5 and Comparative Examples 1 to 7 are shown in Table 1 and Table 2, respectively.

表1实施例1~5的检测结果The detection results of Table 1 Examples 1-5

Figure BDA0002817851530000091
Figure BDA0002817851530000091

Figure BDA0002817851530000101
Figure BDA0002817851530000101

表2对比例1~7的检测结果Table 2 Test results of comparative examples 1 to 7

Figure BDA0002817851530000102
Figure BDA0002817851530000102

由表1中可知,实施例1~5的方法制备的重组苹果脆片的花青素保留率均在86%以上,说明花青素得到了高效的保留,色泽变化率均在1.53以下,说明护色效率极佳;平均孔隙直径和平均壁厚越厚,表明脆片中在质构压缩试验中发生单次崩解的单元越多,反映在质构仪测定上也就是脆度的峰个数越多,而感官体验上则代表样品的酥脆口感越强,产品作为脆片的品质就越高;脆度均在48以上,说明脆度佳。而采用普通的方法(对比例7)制备的重组苹果脆片产品无论是花青素保留率显著减少,还是色泽变化率显著变化,脆度也是显著降低。It can be seen from Table 1 that the anthocyanin retention rates of the recombinant apple crisps prepared by the methods of Examples 1 to 5 are all above 86%, indicating that the anthocyanins are efficiently retained, and the color change rates are all below 1.53, indicating that The color protection efficiency is excellent; the thicker the average pore diameter and the average wall thickness, the more units in the crisp tablet have single disintegration in the texture compression test, which is reflected in the texture analyzer measurement, that is, the peak number of brittleness The higher the number, the stronger the crispy taste of the sample in the sensory experience, and the higher the quality of the product as a crisp; the crispness is above 48, indicating that the crispness is good. However, in the reconstituted apple crisp product prepared by the common method (Comparative Example 7), the anthocyanin retention rate is significantly reduced, the color change rate is significantly changed, and the crispness is also significantly reduced.

由表1和表2中的数据可知,实施例1和对比例1、对比例2、对比例3相比,采用黄酮或阿魏酸、或水代替对羟基苯甲酸乙酯,显著降低了终产品重组苹果脆片的花青素保留率,色泽变化率增大,脆度降低,说明对羟基苯甲酸乙酯的不可替代性。From the data in Table 1 and Table 2, it can be seen that compared with Comparative Example 1, Comparative Example 2, and Comparative Example 3, the use of flavone or ferulic acid or water instead of ethyl p-hydroxybenzoate significantly reduces the final result. The anthocyanin retention rate and color change rate of the product reconstituted apple crisps increased, and the brittleness decreased, indicating the irreplaceability of ethyl paraben.

实施例1和对比例4比较可知,采用水代替低酯果胶,同样显著降低了终产品重组苹果脆片的花青素保留率,色泽变化率增大,脆度降低,说明对羟基苯甲酸乙酯的不可替代性。It can be seen from the comparison of Example 1 and Comparative Example 4 that the use of water instead of low-ester pectin also significantly reduces the anthocyanin retention rate of the final product reconstituted apple crisps, the color change rate increases, and the brittleness decreases, indicating that p-hydroxybenzoic acid The irreplaceability of ethyl esters.

由实施例1和对比例1~4比较可知,是由对羟基苯甲酸乙酯和低酯果胶产生了协同作用,才可以增大花青素保留率,降低色泽变化,并且同时提升产品脆度。From the comparison of Example 1 and Comparative Examples 1 to 4, it can be seen that the synergistic effect of ethyl p-hydroxybenzoate and low-ester pectin can increase the anthocyanin retention rate, reduce the color change, and at the same time improve the brittleness of the product. Spend.

由实施例1和对比例5比较可知,高酯果胶也能与对羟基苯甲酸乙酯产生积极的协同作用,但效果不如低酯果胶佳。It can be seen from the comparison between Example 1 and Comparative Example 5 that high-ester pectin can also produce a positive synergistic effect with ethyl p-hydroxybenzoate, but the effect is not as good as that of low-ester pectin.

由实施例1和对比例6比较可知,充气打浆步骤中,充气可以使得产品脆度得到显著提高。It can be seen from the comparison between Example 1 and Comparative Example 6 that in the aeration beating step, aeration can significantly improve the brittleness of the product.

如图1所示的产品外观图,从左到右,第一行依次为实施例1~5、对比例1六个样品的产品外观图,其花青素保留率分别为92%、86%、88%、90%、89%、70%。第二行依次为对比例2~7六个样品的产品外观图,其花青素保留率分别为75%、62%、72%、80%、88%、43%,实施例1和实施例4的红色色泽最红,正好花青素保留率也最高。As shown in Figure 1, from left to right, the first row is the product appearance of the six samples from Examples 1 to 5 and Comparative Example 1. The anthocyanin retention rates are 92% and 86%, respectively. , 88%, 90%, 89%, 70%. The second row is the product appearance diagram of the six samples of Comparative Examples 2 to 7. The anthocyanin retention rates are 75%, 62%, 72%, 80%, 88%, and 43%, respectively. Example 1 and Example The red color of 4 is the reddest, and the anthocyanin retention rate is also the highest.

尽管本发明的实施方案已公开如上,但其并不仅仅限于说明书和实施方式中所列运用,它完全可以被适用于各种适合本发明的领域,对于熟悉本领域的人员而言,可容易地实现另外的修改,因此在不背离权利要求及等同范围所限定的一般概念下,本发明并不限于特定的细节和这里示出与描述的图例。Although the embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the description and the embodiments, and can be applied to various fields suitable for the present invention. For those skilled in the art, they can easily Therefore, the invention is not limited to the specific details and illustrations shown and described herein without departing from the general concept defined by the appended claims and the scope of equivalents.

Claims (8)

1. The method for improving the texture and color of the vacuum freeze-dried recombined apple crisp is characterized by comprising the following steps:
step one, cleaning apples, peeling, removing kernels, crushing and pulping to obtain apple pulp; the crushing method comprises the following steps: crushing in a low-temperature nitrogen-filled crusher;
step two, adding low-ester pectin in an amount of 10-50 g/kg, calcium chloride in an amount of 1-2 mg/g, p-hydroxybenzoic acid or a derivative thereof in an amount of 0.012-0.5g/kg, and apple peel residue dry powder in an amount of 2-5% by mass into the apple pulp, uniformly mixing, and homogenizing to obtain a mixed solution;
step three, adding organic acid to adjust the pH of the mixed solution in the step two to 3.0-3.8, sterilizing and cooling;
step four, adding a leavening agent according to the ratio of 10-20 g/kg, and fermenting for 48 hours at the temperature of 30 ℃ to obtain a fermentation liquid, wherein the leavening agent is obtained by mixing the compound lactobacillus and the lactobacillus plantarum in a ratio of 2:1, and the bacterial concentration of the leavening agent is 8.0-9.0 Log (CFU/ml);
fifthly, pulping for 2-5 min in an aseptic environment, and filling nitrogen into the fermentation liquor in the pulping process;
sixthly, freezing at-18 ℃ to obtain apple pulp blocks, wherein the thickness of the apple pulp blocks is 3-8 mm;
seventhly, soaking the mixture in a chitosan water solution with the mass fraction of 30-50% at the temperature of-1-0 ℃ for 2-5 seconds, spreading the soaked mixture on a tray at the temperature of-40 ℃, and freezing the spread mixture at the temperature of-18 ℃;
step eight, after freezing, carrying out vacuum freeze drying to obtain apple chips;
wherein, the addition amount of the second step and the fourth step is based on the mass of the apple pulp.
2. The method for improving the texture and color of vacuum freeze-dried reconstituted apple crisps as claimed in claim 1, wherein the parahydroxybenzoic acid derivative comprises: any one or combination of more of ethyl p-hydroxybenzoate, sodium ethyl hydroxybenzoate, propyl p-hydroxybenzoate and sodium methyl p-hydroxybenzoate.
3. The method for improving the texture and color of vacuum freeze-dried reconstituted apple crisps as claimed in claim 1, wherein the sterilization temperature in the third step is 121 ℃ and the sterilization time is 15 min.
4. The method for improving the texture and color of vacuum freeze-dried reconstituted apple crisps as claimed in claim 1, wherein in step six the mold tray is used to hold the apple pulp during freezing.
5. The method for improving the texture and color of the vacuum freeze-dried reconstituted apple crisps as claimed in claim 1, wherein the apple crisps in the step eight are packaged in the dark and oxygen-isolated manner.
6. The method for improving the texture and color of vacuum freeze-dried reconstituted apple crisps according to claim 1, wherein the apple variety is red-fleshed apple.
7. The method for improving the texture and color of the vacuum freeze-dried reconstituted apple crisps as claimed in claim 1, wherein the organic acid is one or a combination of citric acid, malic acid and lactic acid.
8. The method for improving the texture and color of the vacuum freeze-dried reconstituted apple crisp according to claim 1, wherein the compound lactic acid bacteria is one or a combination of bifidobacterium longum, lactobacillus acidophilus, lactobacillus fermentum, lactobacillus helveticus, lactobacillus paracasei, lactobacillus rhamnosus, and streptococcus thermophilus.
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