CN112544919B - Method for improving color and texture quality of vacuum freeze-dried recombined strawberry crisp chips - Google Patents
Method for improving color and texture quality of vacuum freeze-dried recombined strawberry crisp chips Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/01—Instant products; Powders; Flakes; Granules
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
- A23B7/00—Preservation of fruit or vegetables; Chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by group A23B7/08 or A23B7/10
- A23B7/144—Preserving or ripening with chemicals not covered by group A23B7/08 or A23B7/10 in the form of gases, e.g. fumigation; Compositions or apparatus therefor
- A23B7/148—Preserving or ripening with chemicals not covered by group A23B7/08 or A23B7/10 in the form of gases, e.g. fumigation; Compositions or apparatus therefor in a controlled atmosphere, e.g. partial vacuum, comprising only CO2, N2, O2 or H2O
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
- A23B7/00—Preservation of fruit or vegetables; Chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by group A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by group A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
- A23B7/00—Preservation of fruit or vegetables; Chemical ripening of fruit or vegetables
- A23B7/16—Coating with a protective layer; Compositions or apparatus therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/40—Colouring or decolouring of foods
- A23L5/41—Retaining or modifying natural colour by use of additives, e.g. optical brighteners
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
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Abstract
Description
技术领域technical field
本发明涉及食品加工技术领域。更具体地说,本发明涉及一种改善真空冷冻干燥重组草莓脆片色泽和质构品质的方法。The invention relates to the technical field of food processing. More specifically, the present invention relates to a method for improving the color and texture quality of vacuum freeze-dried reconstituted strawberry chips.
背景技术Background technique
近年来,真空冷冻果蔬脆片市场发展迅速。因其具有食用方便、营养健康、已于携带等优点,深受消费者青睐。真空冷冻干燥逐渐成为制备草莓脆片的绿色优质干燥方式。In recent years, the market of vacuum-frozen fruit and vegetable chips has developed rapidly. It is favored by consumers because of its advantages of being convenient to eat, nutritious and healthy, and easy to carry. Vacuum freeze-drying has gradually become a green and high-quality drying method for preparing strawberry chips.
草莓是我国第一大浆果,草莓富含天然色素,其中天竺葵素-3-葡萄糖苷占到80-90%,是草莓红色的主要呈色物质。天竺葵素-3-葡萄糖苷抗氧化性强,具有预防衰老、提高免疫力、降低血脂等多种健康功效。天竺葵素-3-葡萄糖苷容易受到光照、氧气等外界因素影响发生降解,特别是由于真空冷冻干燥红心苹果脆片具有酥松的多孔网络结构,比较面积巨大,这使得氧气、水分和光线更容易接触天竺葵素-3-葡萄糖苷,由此因天竺葵素-3-葡萄糖苷降解而引起的色泽褪变和功能性减弱成为富含天竺葵素-3-葡萄糖苷果蔬脆片贮藏过程难以解决的品质缺陷。长期以来,如何提高果蔬食品中天竺葵素-3-葡萄糖苷的稳定性困扰着食品产业,特别是果蔬脆片加工产业。Strawberry is the largest berry in my country. Strawberry is rich in natural pigments, of which pelargonidin-3-glucoside accounts for 80-90%, which is the main coloring substance of strawberry red. Pelargonium-3-glucoside has strong antioxidant properties, and has various health effects such as preventing aging, improving immunity, and reducing blood lipids. Pelargonium-3-glucoside is easily degraded by external factors such as light and oxygen, especially because the vacuum freeze-dried red heart apple chips have a crisp porous network structure and a relatively large area, which makes oxygen, moisture and light more accessible. Pelargonium-3-glucoside, so the color fade and functional weakening caused by the degradation of pelargonin-3-glucoside become a quality defect that is difficult to solve during the storage process of pelargonin-3-glucoside-rich fruit and vegetable chips. For a long time, how to improve the stability of pelargonidin-3-glucoside in fruit and vegetable food has troubled the food industry, especially the fruit and vegetable chip processing industry.
另一方面,真空冷冻干燥天然果蔬脆片存在硬脆度较低、口感质构绵软的问题。究其原因一是果蔬原料自身组织结构的不均一性,二是果蔬细胞壁干物质含量较少,干燥后难以支撑果蔬脆片多孔网络结构的刚性。On the other hand, vacuum freeze-dried natural fruit and vegetable chips have the problems of low hardness and brittleness and soft texture. One of the reasons is the heterogeneity of the tissue structure of the fruit and vegetable raw materials, and the second is that the dry matter content of the fruit and vegetable cell wall is less, and it is difficult to support the rigidity of the porous network structure of the fruit and vegetable chips after drying.
发明内容SUMMARY OF THE INVENTION
本发明的一个目的是解决至少上述问题,并提供至少后面将说明的优点。An object of the present invention is to solve at least the above-mentioned problems and to provide at least the advantages which will be explained later.
本发明还有一个目的是提供一种改善真空冷冻干燥重组草莓脆片色泽和质构品质的方法,其巧妙地利用食品天然化合物之间的自发化学互作机制,设计了基于细胞壁多糖物质、原儿茶酸(3,4-二羟基苯甲酸)和天竺葵素-3-葡萄糖苷的大小分子相互作用的天竺葵素-3-葡萄糖苷自发稳态化工艺,同时利用质构重组、充气、冻融处理等物理手段精准调控冰晶大小和产品孔隙特征,创制了一种全新的重组草莓脆片,有效解决了产品天竺葵素-3-葡萄糖苷贮藏期不稳定,产品硬脆度不高的问题。Another object of the present invention is to provide a method for improving the color and texture quality of vacuum freeze-dried recombinant strawberry chips. Spontaneous stabilization process of pelargonidin-3-glucoside by the interaction of large and small molecules of catechin (3,4-dihydroxybenzoic acid) and pelargonidin-3-glucoside, using texture reorganization, aeration, freezing and thawing at the same time The size of ice crystals and the pore characteristics of the product are precisely controlled by physical means such as treatment, and a new recombinant strawberry chip is created, which effectively solves the problems of unstable storage period and low hardness and brittleness of the product pelargonidin-3-glucoside.
为了实现根据本发明的这些目的和其它优点,提供了一种改善真空冷冻干燥重组草莓脆片色泽和质构品质的方法,其包括以下步骤:In order to achieve these objects and other advantages according to the present invention, there is provided a method for improving the color and texture quality of vacuum freeze-dried reconstituted strawberry chips, comprising the following steps:
步骤一、将草莓破碎得草莓浆,草莓浆与果胶、原儿茶酸混合均匀,并调节pH至3.0~3.8得物料A;其中,草莓浆与果胶的质量比为1:0.02~0.08,物料A中原儿茶酸的质量浓度为0.1~1.0g/kg;Step 1: Crush the strawberries to obtain strawberry pulp, mix the strawberry pulp with pectin and protocatechuic acid uniformly, and adjust the pH to 3.0~3.8 to obtain material A; wherein, the mass ratio of strawberry pulp to pectin is 1:0.02~0.08 , the mass concentration of protocatechuic acid in material A is 0.1 to 1.0 g/kg;
步骤二、将物料A倒入模具托盘,置于-18℃的冷库中进行冻融处理得块状物料B;Step 2: Pour material A into the mold tray, and place it in a cold storage at -18°C for freeze-thaw treatment to obtain block material B;
步骤三、在物料B表面涂覆一层壳聚糖得物料C;Step 3, coating a layer of chitosan on the surface of material B to obtain material C;
步骤四、将物料C置于真空冷冻干燥箱内干燥,然后充氮包装即得草莓脆片。Step 4: The material C is placed in a vacuum freeze-drying box to dry, and then packed with nitrogen to obtain strawberry chips.
优选的是,所述的改善真空冷冻干燥重组草莓脆片色泽和质构品质的方法,步骤一中,将草莓破碎得草莓浆具体为:选取成熟度均一、无病虫害的草莓洗净,并通过振动筛沥干水分后与苹果皮渣混合,然后置于打浆机中打浆得草莓浆,草莓浆中苹果皮渣的质量分数为5~10%。Preferably, in the method for improving the color and texture quality of vacuum freeze-drying and reconstituting strawberry chips, in step 1, crushing the strawberries to obtain strawberry pulp is specifically as follows: selecting strawberries with uniform maturity and no pests and diseases, washing them, and passing through The vibrating screen is drained and mixed with apple peel residue, and then placed in a beating machine to be beaten to obtain strawberry pulp. The mass fraction of apple peel residue in the strawberry pulp is 5-10%.
优选的是,所述的改善真空冷冻干燥重组草莓脆片色泽和质构品质的方法,步骤一中,将草莓破碎得草莓浆,草莓浆与果胶、原儿茶酸混合均匀,用柠檬酸调节pH至3.0~3.8得物料A。Preferably, in the method for improving the color and texture quality of vacuum freeze-drying and reconstituted strawberry chips, in step 1, the strawberries are crushed to obtain strawberry pulp, the strawberry pulp is uniformly mixed with pectin and protocatechuic acid, and citric acid is used for mixing. Adjust pH to 3.0~3.8 to obtain material A.
优选的是,所述的改善真空冷冻干燥重组草莓脆片色泽和质构品质的方法,步骤二具体为:将物料A均质后倒入模具托盘的模具凹槽内,置于-18℃的冷库中,待物料A冻结后,调节冷库温度:每隔1h将冷库温度上调1℃,直至冷库温度调至-4℃,保温3~6h后将冷库温度再次调至-18℃,按上述冷库温度的调节方式,循环调节1~5次后保温1h取出模具托盘,从模具凹槽内取出块状物得物料B。Preferably, in the method for improving the color and texture quality of vacuum freeze-drying and reconstituting strawberry chips, the second step is specifically: pour the material A into the mold groove of the mold tray after homogenizing, and place it in a temperature of -18°C. In the cold storage, after the material A is frozen, adjust the temperature of the cold storage: increase the temperature of the cold storage by 1 °C every 1 hour until the temperature of the cold storage is adjusted to -4 °C, and then adjust the temperature of the cold storage to -18 °C again after holding for 3 to 6 hours. The temperature adjustment method is to adjust the cycle for 1 to 5 times, then keep the temperature for 1 hour, take out the mold tray, and take out the block from the mold groove to obtain material B.
优选的是,所述的改善真空冷冻干燥重组草莓脆片色泽和质构品质的方法,步骤三具体为:将物料B置于-1~0℃的体积分数为30~50%的壳聚糖溶液中浸泡,2~5s后取出并置于-40℃的托盘上重新放回-18℃的冷库中冻结后取出即得物料C。Preferably, in the method for improving the color and texture quality of vacuum freeze-drying and reconstituted strawberry chips, the third step is specifically: placing material B at -1~0°C with a volume fraction of 30~50% chitosan Soak it in the solution, take it out after 2 to 5 seconds, put it on a tray at -40°C, put it back in a freezer at -18°C, and take it out to get material C.
优选的是,所述的改善真空冷冻干燥重组草莓脆片色泽和质构品质的方法,步骤四具体为:将物料C置于真空冷冻干燥箱内,其中真空干燥箱的冷阱温度-50℃,冷冻干燥箱的置物盘的温度为0~70℃;待物料C的含水量降至7%以下时,将物料C从真空冷冻干燥箱内取出,进行充氮包装,即得草莓脆片。Preferably, in the method for improving the color and texture quality of vacuum freeze-drying and reconstituted strawberry chips, the fourth step is specifically: placing material C in a vacuum freeze-drying oven, wherein the cold trap temperature of the vacuum drying oven is -50°C , the temperature of the storage tray of the freeze-drying box is 0-70°C; when the water content of the material C drops below 7%, the material C is taken out from the vacuum freeze-drying box and packed with nitrogen to obtain strawberry chips.
本发明至少包括以下有益效果:The present invention includes at least the following beneficial effects:
1、本发明首次公布了基于果胶结合原儿茶酸自发化学稳态化的天竺葵素-3-葡萄糖苷护色工艺;通过精心设计基于食品大小分子相互作用的稳态化机制,加上物理氧气阻隔技术,显著提高了产品贮藏期间天竺葵素-3-葡萄糖苷的稳定性。具体来说,该方案利用低甲酯化度果胶分子上丰富的羧基在其等电点以上带负电,天竺葵素-3-葡萄糖苷在等电点以下带正电,由此发生以静电作用为主,氢键和疏水作用为辅的相互结合;与此同时,利用小分子原儿茶酸苯环与天竺葵素-3-葡萄糖苷苯环发生非共价键“Π-Π重叠”,从两面稳定了限制天竺葵素-3-葡萄糖苷的化学结构,使得B环受到空间位阻不能自由活动,同时限制了化学键的解离,大幅提高了天竺葵素-3-葡萄糖苷的化学稳定性;果胶、原儿茶酸均广泛存在于天然果蔬中,本发明利用可以从茶叶、咖啡下脚料等原料中大量提取的原儿茶酸作为护色物质,所制的食品不含人工合成食品添加剂,产品绿色天然;1. The present invention announces for the first time the color protection process of pelargonidin-3-glucoside based on the spontaneous chemical stabilization of pectin combined with protocatechuic acid; Oxygen barrier technology significantly improves the stability of pelargonidin-3-glucoside during product storage. Specifically, this scheme utilizes that the abundant carboxyl groups on the pectin molecule with low methyl esterification degree are negatively charged above its isoelectric point, and pelargonidin-3-glucoside is positively charged below the isoelectric point, thus resulting in electrostatic interaction. At the same time, the non-covalent bond "Π-Π overlap" between the small molecule protocatechuic acid benzene ring and the pelargonidin-3-glucoside benzene ring is used to form the Both sides stabilize the chemical structure that restricts pelargonidin-3-glucoside, so that the B ring cannot move freely due to steric hindrance, and at the same time limits the dissociation of chemical bonds, greatly improving the chemical stability of pelargonidin-3-glucoside; Both gum and protocatechuic acid are widely present in natural fruits and vegetables. The present invention utilizes protocatechuic acid that can be extracted in large quantities from raw materials such as tea leaves and coffee scraps as color-protecting substances, and the prepared food does not contain artificial synthetic food additives. The product is green and natural;
2、本发明通过对草莓块进行壳聚糖涂膜处理,在草莓脆片外围形成了一层果胶覆膜,该层涂膜可以有效阻隔氧气、水分的渗入,维持天竺葵素-3-葡萄糖苷分子周边的低氧、低水分活度环境,进一步抑制天竺葵素-3-葡萄糖苷的降解,有效改善贮藏期间天竺葵素-3-葡萄糖苷的降解问题;壳聚糖涂膜还可降低了草莓脆片的吸湿速率,缓解真空冷冻草莓脆片开袋后因快速吸潮而导致的酥脆性丧失问题;2. In the present invention, a layer of pectin coating is formed on the periphery of the strawberry chips by chitosan coating treatment on the strawberry pieces. The low oxygen and low water activity environment around the glycoside molecule further inhibits the degradation of pelargonidin-3-glucoside and effectively improves the degradation of pelargonidin-3-glucoside during storage; the chitosan coating can also reduce the degradation of strawberry. The moisture absorption rate of crisps can alleviate the problem of crispness loss caused by rapid moisture absorption after vacuum-frozen strawberry crisps are opened;
3、本发明采用缓冻结合冻融处理工艺,使冻结后草莓浆块中的冰晶继续生长,最终形成了较大颗粒冰晶;在冻干过程中,冻结果块外围大颗粒冰晶升华后可形成更大的孔隙率,这些孔隙在进一步可提高干燥速率的同时;更重要的,这种通过物理作用调节冰晶大小的工艺可精准调控冻干果蔬脆片的多孔网络结构,获得具有更大孔隙和更厚孔隙壁的疏松孔隙结构,由此获得更佳酥脆的口感;3. The present invention adopts a slow freezing combined with a freeze-thaw treatment process, so that the ice crystals in the strawberry pulp block continue to grow after freezing, and finally large ice crystals are formed; in the freeze-drying process, the large ice crystals around the frozen fruit block can be formed after sublimation. Larger porosity, these pores can further improve the drying rate; more importantly, this process of adjusting the size of ice crystals through physical action can precisely control the porous network structure of freeze-dried fruit and vegetable chips to obtain larger pores and Loose pore structure with thicker pore walls, resulting in a better crispy mouthfeel;
4、本发明通过加入苹果皮渣膳食纤维,提高了产品的共融点,使得产品可以在更高的温度下升华,加上冻融处理形成的较大孔隙结构有利于水蒸气外散,综合作用可大幅节省干燥时间和生产能耗。4. The present invention improves the fusion point of the product by adding the dietary fiber of apple peel residue, so that the product can be sublimated at a higher temperature, and the larger pore structure formed by the freeze-thaw treatment is conducive to the dispersion of water vapor, and the comprehensive effect Significant savings in drying time and production energy.
本发明的其它优点、目标和特征将部分通过下面的说明体现,部分还将通过对本发明的研究和实践而为本领域的技术人员所理解。Other advantages, objects, and features of the present invention will appear in part from the description that follows, and in part will be appreciated by those skilled in the art from the study and practice of the invention.
具体实施方式Detailed ways
下面结合实施例对本发明做进一步的详细说明,以令本领域技术人员参照说明书文字能够据以实施。The present invention will be further described in detail below with reference to the embodiments, so that those skilled in the art can implement according to the description.
应当理解,本文所使用的诸如“具有”、“包含”以及“包括”术语并不排除一个或多个其它元件或其组合的存在或添加。It should be understood that terms such as "having", "comprising" and "including" as used herein do not exclude the presence or addition of one or more other elements or combinations thereof.
需要说明的是,下述实施方案中所述实验方法,如无特殊说明,均为常规方法,所述试剂和材料,如无特殊说明,均可从商业途径获得。It should be noted that the experimental methods described in the following embodiments are conventional methods unless otherwise specified, and the reagents and materials can be obtained from commercial sources unless otherwise specified.
<实施例1><Example 1>
一种改善真空冷冻干燥重组草莓脆片色泽和质构品质的方法,其包括以下步骤:A method for improving the color and texture quality of vacuum freeze-dried recombinant strawberry chips, comprising the following steps:
预处理:选取成熟度均一、无病虫害的草莓洗净,并通过振动筛沥干水分;Pretreatment: Wash strawberries with uniform maturity and no pests and diseases, and drain the water through a vibrating screen;
混合、打浆:将草莓与苹果皮渣混合,然后置于打浆机中打浆得草莓浆,草莓浆中苹果皮渣的质量分数为5%;。Mixing and beating: mixing strawberries and apple peel residues, and then placing them in a beating machine to obtain strawberry pulp, and the mass fraction of apple peel residues in the strawberry pulp is 5%;
调配:向草莓浆中添加低酯果胶和原儿茶酸(3,4-二羟基苯甲酸),并加入柠檬酸调节pH至3.0得物料A,其中,草莓浆与果胶的质量比为1:0.02,物料A中原儿茶酸的质量浓度为0.1g/kg;Preparation: add low-ester pectin and protocatechuic acid (3,4-dihydroxybenzoic acid) to strawberry pulp, and add citric acid to adjust pH to 3.0 to obtain material A, wherein the mass ratio of strawberry pulp to pectin is 1:0.02, the mass concentration of protocatechuic acid in material A is 0.1g/kg;
均质:将物料A进行均质;Homogenization: Homogenize material A;
再造型:将均质后的物料A倒入模具托盘的模具凹槽中;Remodeling: Pour the homogenized material A into the mold groove of the mold tray;
冻结:将模具托盘置于-18℃的冷库中,缓慢冻结;Freezing: place the mold tray in a cold storage at -18°C and freeze slowly;
冻融处理:调节冷库温度:每隔1h将冷库温度上调1℃,直至冷库温度调至-4℃,保温3~6h后将冷库温度再次调至-18℃,保温1h取出模具托盘,从模具凹槽内取出块状物得物料B;该步骤的目的在于使冻结的草莓块中冰晶持续生长,形成较大颗粒冰晶;Freeze-thaw treatment: adjust the temperature of the cold storage: increase the temperature of the cold storage by 1 °C every 1h until the temperature of the cold storage is adjusted to -4 °C, and then adjust the temperature of the cold storage to -18 °C again after holding for 3 to 6 hours. The block is taken out in the groove to obtain material B; the purpose of this step is to make the ice crystals in the frozen strawberry blocks grow continuously to form larger ice crystals;
涂膜:将物料B置于-1℃的体积分数为30%的壳聚糖溶液中浸泡,2s后取出并置于-40℃的托盘上重新放回-18℃的冷库中冻结后取出即得物料C;Coating: Soak material B in a chitosan solution with a volume fraction of 30% at -1°C, take it out after 2s, put it on a tray at -40°C, put it back in a freezer at -18°C, and then take it out. get material C;
真空冷冻干燥:将物料C置于真空冷冻干燥箱内,其中真空干燥箱的冷阱温度-50℃,冷冻干燥箱的置物盘的温度为0℃;Vacuum freeze-drying: place material C in a vacuum freeze-drying box, wherein the temperature of the cold trap of the vacuum drying box is -50°C, and the temperature of the storage tray of the freeze-drying box is 0°C;
充氮包装:待物料C的含水量降至7%以下时,将物料C从真空冷冻干燥箱内取出,进行充氮包装,即得草莓脆片。充氮包装后的产品至于阴凉、干燥场所贮藏。Nitrogen-filling packaging: When the moisture content of material C drops below 7%, material C is taken out of the vacuum freeze-drying box, and nitrogen-filled packaging is carried out to obtain strawberry chips. The product packed with nitrogen should be stored in a cool and dry place.
<实施例2><Example 2>
一种改善真空冷冻干燥重组草莓脆片色泽和质构品质的方法,其包括以下步骤:A method for improving the color and texture quality of vacuum freeze-dried recombinant strawberry chips, comprising the following steps:
预处理:选取成熟度均一、无病虫害的草莓洗净,并通过振动筛沥干水分;Pretreatment: Wash strawberries with uniform maturity and no pests and diseases, and drain the water through a vibrating screen;
混合、打浆:将草莓与苹果皮渣混合,然后置于打浆机中打浆得草莓浆,草莓浆中苹果皮渣的质量分数为10%;。Mixing and beating: mixing strawberries and apple peel residues, then placing them in a beating machine to obtain strawberry pulp, and the mass fraction of apple peel residues in the strawberry pulp is 10%;
调配:向草莓浆中添加低酯果胶和原儿茶酸(3,4-二羟基苯甲酸),并加入柠檬酸调节pH至3.8得物料A,其中,草莓浆与果胶的质量比为1:0.08,物料A中原儿茶酸的质量浓度为1.0g/kg;Preparation: adding low-ester pectin and protocatechuic acid (3,4-dihydroxybenzoic acid) to strawberry pulp, and adding citric acid to adjust pH to 3.8 to obtain material A, wherein the mass ratio of strawberry pulp and pectin is 1:0.08, the mass concentration of protocatechuic acid in material A is 1.0g/kg;
均质:将物料A进行均质;Homogenization: Homogenize material A;
再造型:将均质后的物料A倒入模具托盘的模具凹槽中;Remodeling: Pour the homogenized material A into the mold groove of the mold tray;
冻结:将模具托盘置于-18℃的冷库中,缓慢冻结;Freezing: place the mold tray in a cold storage at -18°C and freeze slowly;
冻融处理:调节冷库温度:每隔1h将冷库温度上调1℃,直至冷库温度调至-4℃,保温6h后将冷库温度再次调至-18℃,按上述冷库温度的调节方式,循环调节5次(使得冻结的草莓块于-18℃到-4℃波动5个循环,每个循环中冷库温度到达到-4℃时停留6h)后保温1h取出模具托盘,从模具凹槽内取出块状物得物料B;该步骤的目的在于使冻结的草莓块中冰晶持续生长,形成较大颗粒冰晶;Freeze-thaw treatment: Adjust the temperature of the cold storage: increase the temperature of the cold storage by 1 °C every 1h until the temperature of the cold storage is adjusted to -4 °C, and then adjust the temperature of the cold storage to -18 °C again after 6 hours of heat preservation. 5 times (make the frozen strawberry pieces fluctuate at -18°C to -4°C for 5 cycles, and stay for 6h when the temperature of the cold storage reaches -4°C in each cycle), then keep it for 1h, take out the mold tray, and take out the block from the mold groove material B; the purpose of this step is to make ice crystals grow continuously in the frozen strawberry pieces to form larger ice crystals;
涂膜:将物料B置于0℃的体积分数为50%的壳聚糖溶液中浸泡,5s后取出并置于-40℃的托盘上重新放回-18℃的冷库中冻结后取出即得物料C;Coating: Soak material B in a chitosan solution with a volume fraction of 50% at 0°C, take it out after 5s, put it on a tray at -40°C, put it back in a freezer at -18°C, and take it out. material C;
真空冷冻干燥:将物料C置于真空冷冻干燥箱内,其中真空干燥箱的冷阱温度-50℃,冷冻干燥箱的置物盘的温度为70℃;Vacuum freeze-drying: place material C in a vacuum freeze-drying box, wherein the temperature of the cold trap of the vacuum drying box is -50°C, and the temperature of the storage tray of the freeze-drying box is 70°C;
充氮包装:待物料C的含水量降至7%以下时,将物料C从真空冷冻干燥箱内取出,进行充氮包装,即得草莓脆片。充氮包装后的产品至于阴凉、干燥场所贮藏。Nitrogen-filling packaging: When the moisture content of material C drops below 7%, material C is taken out of the vacuum freeze-drying box, and nitrogen-filled packaging is carried out to obtain strawberry chips. The product packed with nitrogen should be stored in a cool and dry place.
<实施例3><Example 3>
一种改善真空冷冻干燥重组草莓脆片色泽和质构品质的方法,其包括以下步骤:A method for improving the color and texture quality of vacuum freeze-dried recombinant strawberry chips, comprising the following steps:
预处理:选取成熟度均一、无病虫害的草莓洗净,并通过振动筛沥干水分;Pretreatment: Wash strawberries with uniform maturity and no pests and diseases, and drain the water through a vibrating screen;
混合、打浆:将草莓与苹果皮渣混合,然后置于打浆机中打浆得草莓浆,草莓浆中苹果皮渣的质量分数为8%;。Mixing and beating: mixing strawberries and apple peel residues, and then placing them in a beating machine to obtain strawberry pulp. The mass fraction of apple peel residues in the strawberry pulp is 8%;
调配:向草莓浆中添加低酯果胶和原儿茶酸(3,4-二羟基苯甲酸),并加入柠檬酸调节pH至3.4得物料A,其中,草莓浆与果胶的质量比为1:0.05,物料A中原儿茶酸的质量浓度为0.5g/kg;Preparation: adding low-ester pectin and protocatechuic acid (3,4-dihydroxybenzoic acid) to strawberry pulp, and adding citric acid to adjust pH to 3.4 to obtain material A, wherein the mass ratio of strawberry pulp and pectin is 1:0.05, the mass concentration of protocatechuic acid in material A is 0.5g/kg;
均质:将物料A进行均质;Homogenization: Homogenize material A;
再造型:将均质后的物料A倒入模具托盘的模具凹槽中;Remodeling: Pour the homogenized material A into the mold groove of the mold tray;
冻结:将模具托盘置于-18℃的冷库中,缓慢冻结;Freezing: place the mold tray in a cold storage at -18°C and freeze slowly;
冻融处理:调节冷库温度:每隔1h将冷库温度上调1℃,直至冷库温度调至-4℃,保温5h后将冷库温度再次调至-18℃,按上述冷库温度的调节方式,循环调节3次(使得冻结的草莓块于-18℃到-4℃波动3个循环,每个循环中冷库温度到达到-4℃时停留5h)后保温1h取出模具托盘,从模具凹槽内取出块状物得物料B;该步骤的目的在于使冻结的草莓块中冰晶持续生长,形成较大颗粒冰晶;Freeze-thaw treatment: Adjust the temperature of the cold storage: increase the temperature of the cold storage by 1°C every 1 hour until the temperature of the cold storage is adjusted to -4 °C, and then adjust the temperature of the cold storage to -18 °C again after 5 hours of heat preservation. 3 times (make the frozen strawberry pieces fluctuate from -18°C to -4°C for 3 cycles, and stay for 5h when the temperature of the cold storage reaches -4°C in each cycle), then keep it for 1h, take out the mold tray, and take out the block from the mold groove material B; the purpose of this step is to make ice crystals grow continuously in the frozen strawberry pieces to form larger ice crystals;
涂膜:将物料B置于-0.5℃的体积分数为40%的壳聚糖溶液中浸泡,4s后取出并置于-40℃的托盘上重新放回-18℃的冷库中冻结后取出即得物料C;Coating: Soak material B in a chitosan solution with a volume fraction of 40% at -0.5°C, take it out after 4s, put it on a tray at -40°C, put it back in a freezer at -18°C, and then take it out. get material C;
真空冷冻干燥:将物料C置于真空冷冻干燥箱内,其中真空干燥箱的冷阱温度-50℃,冷冻干燥箱的置物盘的温度为35℃;Vacuum freeze-drying: Place material C in a vacuum freeze-drying box, where the temperature of the cold trap of the vacuum-drying box is -50°C, and the temperature of the storage tray of the freeze-drying box is 35°C;
充氮包装:待物料C的含水量降至7%以下时,将物料C从真空冷冻干燥箱内取出,进行充氮包装,即得草莓脆片。充氮包装后的产品至于阴凉、干燥场所贮藏。Nitrogen-filling packaging: When the moisture content of material C drops below 7%, material C is taken out of the vacuum freeze-drying box, and nitrogen-filled packaging is carried out to obtain strawberry chips. The product packed with nitrogen should be stored in a cool and dry place.
<对比例1><Comparative Example 1>
一种改善真空冷冻干燥重组草莓脆片色泽和质构品质的方法,与实施例3的不同在于,调配步骤中草莓浆中加入柠檬酸调pH至3.4得物料A;即物料A中不加入果胶和原儿茶酸,其余条件和参数同实施例3。A method for improving the color and texture quality of vacuum freeze-dried reconstituted strawberry chips, which is different from Example 3 in that in the blending step, citric acid is added to the strawberry pulp to adjust the pH to 3.4 to obtain material A; that is, no fruit is added to material A. Gum and protocatechuic acid, other conditions and parameters are the same as in Example 3.
<对比例2><Comparative Example 2>
一种改善真空冷冻干燥重组草莓脆片色泽和质构品质的方法,与实施例3的不同在于,调配步骤中物料A中不加入果胶,其余条件和参数同实施例3。A method for improving the color and texture quality of vacuum freeze-dried reconstituted strawberry chips is different from Example 3 in that no pectin is added to material A in the compounding step, and other conditions and parameters are the same as those of Example 3.
<对比例3><Comparative Example 3>
一种改善真空冷冻干燥重组草莓脆片色泽和质构品质的方法,与实施例3的不同在于,调配步骤中物料A中不加入原儿茶酸,其余条件和参数同实施例3。A method for improving the color and texture quality of vacuum freeze-dried reconstituted strawberry chips is different from Example 3 in that no protocatechuic acid is added to material A in the compounding step, and other conditions and parameters are the same as those of Example 3.
<对比例4><Comparative Example 4>
一种改善真空冷冻干燥重组草莓脆片色泽和质构品质的方法,与实施例3的不同在于,调配步骤中用黄酮代替原儿茶酸,物料A中黄酮的质量浓度为0.1~1.0g/kg,其余条件和参数同实施例3。A method for improving the color and texture quality of vacuum freeze-dried recombinant strawberry chips, which is different from Example 3 in that flavonoids are used to replace protocatechuic acid in the compounding step, and the mass concentration of flavonoids in material A is 0.1-1.0 g/ kg, and other conditions and parameters are the same as in Example 3.
<对比例5><Comparative Example 5>
一种改善真空冷冻干燥重组草莓脆片色泽和质构品质的方法,与实施例3的不同在于,调配步骤中用咖啡酸代替原儿茶酸,物料A中咖啡酸的质量浓度为0.1~1.0g/kg,其余条件和参数同实施例3。A method for improving the color and texture quality of vacuum freeze-dried reconstituted strawberry chips, which is different from Example 3 in that caffeic acid is used instead of protocatechuic acid in the compounding step, and the mass concentration of caffeic acid in material A is 0.1-1.0 g/kg, and other conditions and parameters are the same as in Example 3.
<对比例6><Comparative Example 6>
一种改善真空冷冻干燥重组草莓脆片色泽和质构品质的方法,与实施例3的不同在于,物料A经过冻结步骤后缓慢冻结,直接进入涂膜步骤,即无冻融处理步骤;其余条件和参数同实施例3。A method for improving the color and texture quality of vacuum freeze-dried and reconstituted strawberry chips is different from Example 3 in that material A is slowly frozen after the freezing step, and directly enters the film coating step, that is, there is no freeze-thaw treatment step; other conditions and parameters are the same as in Example 3.
<对比例7><Comparative Example 7>
一种改善真空冷冻干燥重组草莓脆片色泽和质构品质的方法,与实施例3的不同在于,将物料B直接置于真空冷冻干燥箱内进行真空冷冻干燥,即不经过涂膜步骤;其余条件和参数同实施例3。A method for improving the color and texture quality of the reconstituted strawberry chips by vacuum freeze-drying is different from Example 3 in that material B is directly placed in a vacuum freeze-drying box for vacuum freeze-drying, that is, without the coating step; Conditions and parameters are the same as in Example 3.
<对比例8><Comparative Example 8>
一种改善真空冷冻干燥重组草莓脆片色泽和质构品质的方法,与实施例3的不同在于,步骤混合、打浆步骤中,直接将草莓置于打浆机中进行打浆得草莓浆,即不加入苹果皮渣,其余条件和参数同实施例3。A method for improving the color and texture quality of vacuum freeze-drying and reconstituted strawberry chips is different from Example 3 in that in the steps of mixing and beating, the strawberries are directly placed in a beating machine to be beaten to obtain strawberry pulp, that is, without adding Apple peel residue, other conditions and parameters are the same as Example 3.
<对比例9><Comparative Example 9>
一种改善真空冷冻干燥重组草莓脆片色泽和质构品质的方法,其包括以下步骤:A method for improving the color and texture quality of vacuum freeze-dried recombinant strawberry chips, comprising the following steps:
预处理:选取成熟度均一、无病虫害的草莓洗净,并通过振动筛沥干水分;Pretreatment: Wash strawberries with uniform maturity and no pests and diseases, and drain the water through a vibrating screen;
打浆:将草莓置于打浆机中打浆、均质得物料A;Beating: put the strawberries in a beater to beat and homogenize to obtain material A;
真空冷冻干燥:将物料A置于真空冷冻干燥箱内,其中真空干燥箱的冷阱温度-50℃,冷冻干燥箱的置物盘的温度为35℃;Vacuum freeze-drying: place material A in a vacuum freeze-drying box, wherein the temperature of the cold trap of the vacuum drying box is -50°C, and the temperature of the storage tray of the freeze-drying box is 35°C;
充氮包装:待物料A的含水量降至7%以下时,将物料A从真空冷冻干燥箱内取出,进行充氮包装,即得草莓脆片。充氮包装后的产品至于阴凉、干燥场所贮藏。Nitrogen-filled packaging: When the moisture content of material A drops below 7%, take out material A from the vacuum freeze-drying box, and carry out nitrogen-filled packaging to obtain strawberry chips. The product packed with nitrogen should be stored in a cool and dry place.
<试验例><Test example>
1、检测本发明实施例1~3和对比例1~9制备得到的草莓脆片的品质:1. Detect the quality of the strawberry chips prepared in Examples 1-3 of the present invention and Comparative Examples 1-9:
1.1、草莓脆片中天竺葵素-3-葡萄糖苷保留率测定1.1. Determination of retention rate of pelargonidin-3-glucoside in strawberry chips
采用高效液相色谱-质谱联用HPLC-MS法测定样品中总花青素含量。使用2%盐酸甲醇溶液提取样品中的天竺葵素-3-葡萄糖苷,过滤后使用液相法测定。通过标准品获得的标准曲线计算天竺葵素-3-葡萄糖苷含量和保留率。The content of total anthocyanins in the samples was determined by high performance liquid chromatography-mass spectrometry coupled with HPLC-MS. The pelargonidin-3-glucoside in the sample was extracted with 2% hydrochloric acid methanol solution, filtered and determined by liquid phase method. Pelargonium-3-glucoside content and retention were calculated from the standard curve obtained from the standard.
1.2、草莓脆片的色差值的测定1.2. Determination of color difference value of strawberry chips
采用色彩色差仪测定果蔬脆片的色泽,本实验用△L、△a、△b、△E值代表被测样品的色泽(L、a、b)与鲜样的(L*、a*、b*)的色差值。△E计算方法如下:The color of the fruit and vegetable chips was measured by a colorimeter. In this experiment, the △L, △a, △b, and △E values were used to represent the color (L, a, b) of the tested sample and the fresh sample (L*, a*, b*) color difference value. The calculation method of △E is as follows:
式中,L、L*分别为鲜果和干燥后脆片的明度值;a、a*分别是鲜果和干燥后脆片的红绿值;b和b*分别是鲜果和干燥后脆片的黄蓝值;ΔL为总色差值。In the formula, L and L* are the brightness values of fresh fruit and dried chips, respectively; a, a* are the red and green values of fresh fruit and dried chips, respectively; b and b* are the yellowness of fresh fruit and dried chips, respectively. Blue value; ΔL is the total color difference value.
1.3、草莓脆片中膳食纤维含量测定1.3. Determination of dietary fiber content in strawberry chips
采用《GB/T 5009.88-2008食品中膳食纤维的测定》中的称重法测定膳食纤维含量。The content of dietary fiber was determined by the weighing method in "GB/T 5009.88-2008 Determination of Dietary Fiber in Foods".
1.4、共融点测定1.4. Determination of the fusion point
采用电阻法测定物料共融点。将果浆置于-40℃条件下完全冻结,然后逐步升高温度。期间使用电阻仪持续检测物料的电阻,当电阻开始呈现急速减小时的温度即为物料的共融点。The material eutectic point was determined by resistance method. Freeze the pulp completely at -40°C, then gradually increase the temperature. During the period, the resistance meter is used to continuously detect the resistance of the material. When the resistance begins to decrease rapidly, the temperature is the eutectic point of the material.
1.5、孔隙特征分析1.5. Analysis of Pore Characteristics
采用微-CT扫描进行,采用微-CT自带分析软件分析后获得的草莓脆片的内部平均孔隙直径和孔隙壁厚。The micro-CT scan was used, and the internal average pore diameter and pore wall thickness of the strawberry chips obtained after analysis by the micro-CT self-contained analysis software.
1.6、硬脆度的测定1.6. Determination of hardness and brittleness
用TA-XT2i/50型物性测定仪测定,选取形状、大小相近的草莓脆片进行质构测定。从包装中迅速取出样品后,用物性测定仪做削切试验,重复10次,最后取平均值。脆度用测试产生峰数的多少来表示,单位为“个”。测试数值在一定范围内呈正相关增长,峰数越多,则产品的酥脆度越好,反之则产品的酥脆度越差。Measured with TA-XT2i/50 type physical property analyzer, selected strawberry chips with similar shape and size for texture determination. After quickly taking out the sample from the package, use the physical property analyzer to do the cutting test, repeat 10 times, and finally take the average value. The brittleness is expressed by the number of peaks produced by the test, and the unit is "pieces". The test value increases in a positive correlation within a certain range. The more peaks, the better the crispness of the product, and vice versa, the worse the crispness of the product.
1.7、保质期判定1.7. Determination of shelf life
将包装后的草莓脆片至于25℃条件下贮藏,跟踪产品品质,以产品天竺葵素-3-葡萄糖苷降解50%以上,或者a值下降20%以上作为保质期终点。The packaged strawberry chips were stored at 25°C, the product quality was tracked, and the product pelargonidin-3-glucoside was degraded by more than 50%, or the a value decreased by more than 20% as the end of the shelf life.
2、检测结果分析2. Analysis of test results
本发明实施例1~3和对比例1~9的检测结果如表1所示:The detection results of Examples 1 to 3 of the present invention and Comparative Examples 1 to 9 are shown in Table 1:
表1检测结果Table 1 Test results
由表1可知,相较于常规技术对比例9,本发明实施例1~3得到的草莓脆片的天竺葵素-3-葡萄糖苷的保留率均高达90%以上,色差较小,膳食纤维的含量较高,且具有较高的平均孔隙直径和评价壁厚,脆度也达到了45以上,保质期长达18个月,由此说明本发明创制了一种全新的重组草莓脆片,有效解决了产品天竺葵素-3-葡萄糖苷贮藏期不稳定,产品硬脆度不高的问题。As can be seen from Table 1, compared with the conventional technology Comparative Example 9, the retention rates of pelargonidin-3-glucoside of the strawberry chips obtained in Examples 1 to 3 of the present invention are all as high as more than 90%, the color difference is small, and the dietary fiber content is small. The content is high, the average pore diameter and the evaluation wall thickness are relatively high, the brittleness has reached more than 45, and the shelf life is as long as 18 months. It solves the problems of unstable storage period of the product pelargonidin-3-glucoside and low hardness and brittleness of the product.
比较实施例3与对比例1~5的数据可知,本发明在草莓浆中加入果胶和原儿茶酸,基于果胶结合原儿茶酸自发化学稳态化的天竺葵素-3-葡萄糖苷护色工艺;通过精心设计基于食品大小分子相互作用的稳态化机制,加上物理氧气阻隔技术,显著提高了产品贮藏期间天竺葵素-3-葡萄糖苷的稳定性。Comparing the data of Example 3 and Comparative Examples 1 to 5, it can be seen that the present invention adds pectin and protocatechuic acid to strawberry pulp, and based on pectin combined with protocatechuic acid, the spontaneous chemical stabilization of pelargonidin-3-glucoside Color protection process; The stability of pelargonidin-3-glucoside during product storage is significantly improved through careful design of the homeostasis mechanism based on the interaction of large and small molecules in food, coupled with physical oxygen barrier technology.
比较实施例3与对比例6的数据可知,本发明采用缓冻结合冻融处理工艺,使冻结后草莓浆块中的冰晶继续生长,最终形成了较大颗粒冰晶;在冻干过程中,冻结果块外围大颗粒冰晶升华后可形成更大的孔隙率,这些孔隙在进一步可提高干燥速率的同时;更重要的,这种通过物理作用调节冰晶大小的工艺可精准调控冻干果蔬脆片的多孔网络结构,获得具有更大孔隙和更厚孔隙壁的疏松孔隙结构,由此获得更佳酥脆的口感;Comparing the data of Example 3 and Comparative Example 6, it can be seen that the present invention adopts the slow freezing combined with the freeze-thaw treatment process, so that the ice crystals in the frozen strawberry pulp continue to grow, and finally large ice crystals are formed; As a result, larger porosity can be formed after the sublimation of large ice crystals on the periphery of the block. These pores can further improve the drying rate. More importantly, this process of adjusting the size of ice crystals through physical action can precisely control the freeze-dried fruit and vegetable chips. Porous network structure to obtain a loose pore structure with larger pores and thicker pore walls, thereby obtaining a better crispy taste;
比较实施例3与对比例7的数据可知,本发明通过对草莓块进行壳聚糖涂膜处理,在草莓脆片外围形成了一层果胶覆膜,该层涂膜可以有效阻隔氧气、水分的渗入,维持天竺葵素-3-葡萄糖苷分子周边的低氧、低水分活度环境,进一步抑制天竺葵素-3-葡萄糖苷的降解,有效改善贮藏期间天竺葵素-3-葡萄糖苷的降解问题;壳聚糖涂膜还可降低了草莓脆片的吸湿速率,缓解真空冷冻草莓脆片开袋后因快速吸潮而导致的酥脆性丧失问题;Comparing the data of Example 3 and Comparative Example 7, it can be seen that the present invention forms a layer of pectin coating on the periphery of the strawberry chips by carrying out the chitosan coating treatment on the strawberry pieces, which can effectively block oxygen and moisture. The infiltration of pelargonidin-3-glucoside maintains a low oxygen and low water activity environment around the pelargonidin-3-glucoside molecule, further inhibits the degradation of pelargonidin-3-glucoside, and effectively improves the degradation of pelargonidin-3-glucoside during storage; Chitosan coating can also reduce the moisture absorption rate of strawberry chips and alleviate the problem of crispness loss caused by rapid moisture absorption after vacuum-frozen strawberry chips are opened;
比较实施例3与对比例8的数据可知,本发明通过加入苹果皮渣膳食纤维,提高了产品的共融点,使得产品可以在更高的温度下升华,加上冻融处理形成的较大孔隙结构有利于水蒸气外散,综合作用可大幅节省干燥时间和生产能耗。Comparing the data of Example 3 and Comparative Example 8, it can be seen that the present invention improves the fusion point of the product by adding the dietary fiber of apple peel residue, so that the product can be sublimated at a higher temperature, and the larger pores formed by the freeze-thaw treatment are added. The structure is conducive to the dissipation of water vapor, and the comprehensive effect can greatly save drying time and production energy consumption.
这里说明的设备数量和处理规模是用来简化本发明的说明的。对本发明的应用、修改和变化对本领域的技术人员来说是显而易见的。The number of apparatuses and processing scales described here are intended to simplify the description of the present invention. Applications, modifications and variations to the present invention will be apparent to those skilled in the art.
尽管本发明的实施方案已公开如上,但其并不仅仅限于说明书和实施方式中所列运用,它完全可以被适用于各种适合本发明的领域,对于熟悉本领域的人员而言,可容易地实现另外的修改,因此在不背离权利要求及等同范围所限定的一般概念下,本发明并不限于特定的细节。Although the embodiment of the present invention has been disclosed as above, it is not limited to the application listed in the description and the embodiment, and it can be applied to various fields suitable for the present invention. For those skilled in the art, it can be easily Therefore, the invention is not limited to the specific details without departing from the general concept defined by the appended claims and the scope of equivalents.
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