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CN112384632A - 对多核苷酸进行测序的方法 - Google Patents

对多核苷酸进行测序的方法 Download PDF

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Publication number
CN112384632A
CN112384632A CN201880095214.8A CN201880095214A CN112384632A CN 112384632 A CN112384632 A CN 112384632A CN 201880095214 A CN201880095214 A CN 201880095214A CN 112384632 A CN112384632 A CN 112384632A
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Prior art keywords
nucleotide
chemiluminescent
nucleotides
chemiluminescent label
luminescence
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CN201880095214.8A
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CN112384632B (zh
Inventor
廖莎
陈奥
章文蔚
徐崇钧
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Qingdao Huada Zhizao Technology Co ltd
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Shenzhen Huada Zhizaojichuang Technology Co ltd
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Priority to CN202411777713.9A priority patent/CN119432989A/zh
Publication of CN112384632A publication Critical patent/CN112384632A/zh
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

提供一种用于确定靶单链多核苷酸的序列的方法，包括监测与靶单链多核苷酸互补的核苷酸的依次掺入，其中所述核苷酸各自附接至引发不同发光动力学或发光类型的化学发光标记物，其中每个掺入的核苷酸通过检测所述化学发光标记物参与的化学发光反应的发光动力学或发光类型和随后除去所述化学发光标记物来鉴别。

Description

PCT国内申请，说明书已公开。

Claims (13)

  1. PCT国内申请，权利要求书已公开。
CN201880095214.8A 2018-10-11 2018-10-11 对多核苷酸进行测序的方法 Active CN112384632B (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202411777709.2A CN119753111A (zh) 2018-10-11 2018-10-11 对多核苷酸进行测序的方法
CN202411777713.9A CN119432989A (zh) 2018-10-11 2018-10-11 对多核苷酸进行测序的方法

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PCT/CN2018/109809 WO2020073274A1 (zh) 2018-10-11 2018-10-11 对多核苷酸进行测序的方法

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CN202411777709.2A Division CN119753111A (zh) 2018-10-11 2018-10-11 对多核苷酸进行测序的方法

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CN112384632B CN112384632B (zh) 2024-12-27

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024067674A1 (zh) * 2022-09-28 2024-04-04 深圳华大智造科技股份有限公司 测序方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030104437A1 (en) * 2001-12-04 2003-06-05 Colin Barnes Labelled Nucleotides
US20040171040A1 (en) * 2002-11-22 2004-09-02 Veeraiah Bodepudi Detectable labeled nucleoside analogs and methods of use thereof
EP1681357A2 (en) * 1999-05-19 2006-07-19 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
CN1973048A (zh) * 2003-02-05 2007-05-30 通用电气医疗集团生物科学公司 末端磷酸根标记的核苷酸和使用方法
US9951385B1 (en) * 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
CN108251516A (zh) * 2017-12-06 2018-07-06 上海交通大学 Dna单分子测序方法与测序系统

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2744064C (en) * 2008-11-18 2021-09-14 Bionanomatrix, Inc. Polynucleotide mapping and sequencing
CN101942000A (zh) * 2010-04-13 2011-01-12 深圳华因康基因科技有限公司 携带修饰物的核苷酸及其制备方法和用于基因测序的方法
CN102329884B (zh) * 2011-10-20 2013-05-08 东南大学 两核苷酸同时合成dna测序方法及其应用
CN103484106B (zh) * 2013-09-05 2015-08-19 上海交通大学 四色荧光标记可逆终端及其在dna测序中的用途

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1681357A2 (en) * 1999-05-19 2006-07-19 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US20030104437A1 (en) * 2001-12-04 2003-06-05 Colin Barnes Labelled Nucleotides
US20040171040A1 (en) * 2002-11-22 2004-09-02 Veeraiah Bodepudi Detectable labeled nucleoside analogs and methods of use thereof
CN1973048A (zh) * 2003-02-05 2007-05-30 通用电气医疗集团生物科学公司 末端磷酸根标记的核苷酸和使用方法
US9951385B1 (en) * 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
CN108251516A (zh) * 2017-12-06 2018-07-06 上海交通大学 Dna单分子测序方法与测序系统

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
袁津玮: "核酸分子的化学发光标记和化学发光检测", 国外医学.分子生物学分册, no. 05, pages 197 - 202 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024067674A1 (zh) * 2022-09-28 2024-04-04 深圳华大智造科技股份有限公司 测序方法

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CN119753111A (zh) 2025-04-04
WO2020073274A1 (zh) 2020-04-16
CN112384632B (zh) 2024-12-27
CN119432989A (zh) 2025-02-14

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