CN112345504A - Integrated fluorescence detection device - Google Patents
Integrated fluorescence detection device Download PDFInfo
- Publication number
- CN112345504A CN112345504A CN202011194047.8A CN202011194047A CN112345504A CN 112345504 A CN112345504 A CN 112345504A CN 202011194047 A CN202011194047 A CN 202011194047A CN 112345504 A CN112345504 A CN 112345504A
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- cylindrical frame
- fluorescence
- opening
- lens
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- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 11
- 239000000126 substance Substances 0.000 claims abstract description 10
- 230000003287 optical effect Effects 0.000 claims abstract description 8
- 230000005284 excitation Effects 0.000 claims abstract description 3
- 238000001914 filtration Methods 0.000 claims abstract description 3
- 238000001514 detection method Methods 0.000 abstract description 6
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 241000711573 Coronaviridae Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229910044991 metal oxide Inorganic materials 0.000 description 1
- 150000004706 metal oxides Chemical class 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
- G01N2021/6467—Axial flow and illumination
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
- G01N2021/6471—Special filters, filter wheel
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- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses an integrated fluorescence detection device, which is horizontally aligned with a sample and comprises: a cylindrical frame with one end opened and horizontally facing the sample; the light sources are fixed on the inner side edge of the opening of the cylindrical frame at equal intervals and used for emitting light; a plurality of condenser lenses which are fixed at the inner side edge of the opening of the cylindrical frame at equal intervals, focus the light of each light source and irradiate the surface of the sample; the lens is vertically fixed at the opening of the cylindrical frame at the inner side of the light source and used for converging fluorescence emitted by the excitation of fluorescent substances of the sample; the optical filter is vertically fixed at the opening of the cylindrical frame at the inner side of the lens and is used for filtering the fluorescence from the lens; and the cmos chip is fixed at the opening of the cylindrical frame on the inner side of the optical filter and used for collecting a fluorescence signal from the filtered fluorescence. The miniature light source and the detection are integrated into a whole, and the portable light source is convenient to carry.
Description
Technical Field
The present invention relates to a fluorescence detection device.
Background
With the abuse of new coronavirus in the world, the requirement of rapid and portable nucleic acid detection becomes more and more urgent, the portable field detection has higher requirements on the volume and the weight of equipment, and the traditional light source and detection mode for detecting nucleic acid are not easy to make the light source and the detection have small volume, so the device is not easy to carry.
Disclosure of Invention
The invention aims to provide an integrated fluorescence detection device, which integrates a micro light source and detection into a whole and is convenient to carry.
The technical scheme for realizing the purpose is as follows:
an integrated fluorescence detection device, horizontally aligned with a sample, comprising:
a cylindrical frame with one end opened and horizontally facing the sample;
the light sources are fixed on the inner side edge of the opening of the cylindrical frame at equal intervals and used for emitting light;
a plurality of condenser lenses which are fixed at the inner side edge of the opening of the cylindrical frame at equal intervals, focus the light of each light source and irradiate the surface of the sample;
the lens is vertically fixed at the opening of the cylindrical frame at the inner side of the light source and used for converging fluorescence emitted by the excitation of fluorescent substances of the sample;
the optical filter is vertically fixed at the opening of the cylindrical frame at the inner side of the lens and is used for filtering the fluorescence from the lens; and
and a cmos (complementary metal oxide semiconductor) chip fixed at the opening of the cylindrical frame inside the optical filter and collecting a fluorescence signal from the filtered fluorescence.
Preferably, the method further comprises the following steps: and the control switch is arranged in the cylindrical frame and used for turning on/off the light source.
Preferably, the sample is vertically placed, and the condensing lens and the vertical plane of the sample form a preset angle; under the preset angle, the fluorescent substance of the sample is excited to emit fluorescence level to the lens;
preferably, the number of the light sources and the number of the condenser lenses are 4, and the light sources and the condenser lenses correspond to each other one by one.
The invention has the beneficial effects that: the light source and the cmos chip are integrated into a whole, light is gathered on the surface of the sample, fluorescence emitted by the fluorescent substance of the sample is collected and filtered, and a fluorescence signal is collected by the cmos chip, so that the cmos chip is light and convenient to carry.
Drawings
FIG. 1 is a block diagram of an integrated fluorescence detection device of the present invention;
fig. 2 is a schematic view of the arrangement of light sources in the present invention.
Detailed Description
The invention will be further explained with reference to the drawings.
Referring to fig. 1 and 2, the integrated fluorescence detecting apparatus of the present invention, which is horizontally aligned with a sample 100, includes: the device comprises a cylindrical frame 1, a plurality of light sources 2, a plurality of condenser lenses 3, a lens 4, a filter 5, a cmos chip 6 and a control switch 7.
The sample 100 is placed vertically, and one end of the cylindrical frame 1 is opened and horizontal, and faces the sample 100. The plurality of light sources 2 are fixed at the inner side edge of the opening of the cylindrical frame 1 at equal intervals and used for emitting light. The plurality of condensing lenses 3 correspond to the light sources 2 one by one, are fixed at the inner side edge of the opening of the cylindrical frame 1 at equal intervals, and focus the light of each light source 2 and irradiate the surface of the sample 100. The condensing lens 3 and the vertical plane of the sample 100 form a preset angle; at this predetermined angle, the fluorescent substance of the sample 100 is excited to emit a level of fluorescence toward the lens 4. In this embodiment, the number of the light sources 2 and the number of the condenser lenses 3 are both 4, and the light sources 2 are implemented by using laser light source chips.
The surface of the sample 100 is irradiated, and the fluorescent substance of the sample is excited to emit a specific fluorescence of a certain wavelength. The lens 4 is vertically fixed at the opening of the cylindrical frame 1 at the inner side of the light source 2, and converges the fluorescence emitted by the fluorescent substance of the sample. The optical filter 5 is vertically fixed at the opening of the cylindrical frame 1 at the inner side of the lens 4, and filters the fluorescence from the lens 4. The cmos chip 6 is fixed to the opening of the cylindrical frame 1 inside the optical filter 5, and collects a fluorescence signal from the filtered fluorescence. The cmos chip 6 is realized by using an existing product, and the acquisition of a fluorescence signal can be performed. In this embodiment, the cmos chip 6 is model IMX236 LQ.
A control switch 7 is provided in the cylindrical frame 1 for turning on/off the light source 2. Of course, the invention is also provided with a power supply for supplying power to the cmos chip 6 and the light source 2, and a storage battery is generally adopted.
Thus, the light source 2 is turned on, light is irradiated onto the surface of the sample 100, fluorescence emitted by the fluorescent substance of the sample 100 being excited is filtered by the filter 5 through the lens 4, and a fluorescence signal is collected from the filtered fluorescence by the cmos chip 6.
The above embodiments are provided only for illustrating the present invention and not for limiting the present invention, and those skilled in the art can make various changes and modifications without departing from the spirit and scope of the present invention, and therefore all equivalent technical solutions should also fall within the scope of the present invention, and should be defined by the claims.
Claims (4)
1. An integrated fluorescence detection device, horizontally aligned with a sample, comprising:
a cylindrical frame with one end opened and horizontally facing the sample;
the light sources are fixed on the inner side edge of the opening of the cylindrical frame at equal intervals and used for emitting light;
a plurality of condenser lenses which are fixed at the inner side edge of the opening of the cylindrical frame at equal intervals, focus the light of each light source and irradiate the surface of the sample;
the lens is vertically fixed at the opening of the cylindrical frame at the inner side of the light source and used for converging fluorescence emitted by the excitation of fluorescent substances of the sample;
the optical filter is vertically fixed at the opening of the cylindrical frame at the inner side of the lens and is used for filtering the fluorescence from the lens; and
and the cmos chip is fixed at the opening of the cylindrical frame on the inner side of the optical filter and used for collecting a fluorescence signal from the filtered fluorescence.
2. The integrated fluorescence detection device of claim 1, further comprising: and the control switch is arranged in the cylindrical frame and used for turning on/off the light source.
3. The integrated fluorescence detection device of claim 1, wherein the sample is positioned vertically, and the collection mirror is at a predetermined angle from the vertical of the sample; at the preset angle, the fluorescent substance of the sample is excited to emit a fluorescence level towards the lens.
4. The integrated fluorescence detection device of claim 1, wherein the number of the light sources and the condenser lens is 4, and the light sources and the condenser lens correspond to each other.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011194047.8A CN112345504A (en) | 2020-10-30 | 2020-10-30 | Integrated fluorescence detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011194047.8A CN112345504A (en) | 2020-10-30 | 2020-10-30 | Integrated fluorescence detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112345504A true CN112345504A (en) | 2021-02-09 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011194047.8A Pending CN112345504A (en) | 2020-10-30 | 2020-10-30 | Integrated fluorescence detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112345504A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102590168A (en) * | 2012-02-16 | 2012-07-18 | 无锡迈通科学仪器有限公司 | Light emitting diode-based fluorescent microorganism detector |
| CN104204778A (en) * | 2012-03-12 | 2014-12-10 | 三菱丽阳株式会社 | Fluorescence detection device and fluorescence detection method |
| CN104614354A (en) * | 2015-01-29 | 2015-05-13 | 北京海维尔科技发展有限公司 | Fluorescence imaging device and method |
-
2020
- 2020-10-30 CN CN202011194047.8A patent/CN112345504A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102590168A (en) * | 2012-02-16 | 2012-07-18 | 无锡迈通科学仪器有限公司 | Light emitting diode-based fluorescent microorganism detector |
| CN104204778A (en) * | 2012-03-12 | 2014-12-10 | 三菱丽阳株式会社 | Fluorescence detection device and fluorescence detection method |
| CN104614354A (en) * | 2015-01-29 | 2015-05-13 | 北京海维尔科技发展有限公司 | Fluorescence imaging device and method |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210209 |
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| RJ01 | Rejection of invention patent application after publication |