CN104614354A - Fluorescence imaging device and method - Google Patents
Fluorescence imaging device and method Download PDFInfo
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- CN104614354A CN104614354A CN201510046546.5A CN201510046546A CN104614354A CN 104614354 A CN104614354 A CN 104614354A CN 201510046546 A CN201510046546 A CN 201510046546A CN 104614354 A CN104614354 A CN 104614354A
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Abstract
The invention relates to a fluorescence imaging device and method. The device comprises at least one group of laser sources, a to-be-tested sample, an optical color filter, an imaging lens, an image sensor and an intelligent terminal. An optical filter is positioned above the to-be-tested sample and fixedly arranged on the lower end of the imaging lens, the image sensor is fixedly arranged on the upper end of the imaging lens and connected with the intelligent terminal; the laser sources emit laser to be irradiated onto the to-be-tested sample and generate fluorescence which is irradiated onto the optical color filter, only fluorescence with specific wavelength in the fluorescence can penetrate through the optical color filter and irradiate into the imaging lens, and is focused on the image sensor by the imaging lens. According to the fluorescence imaging device, the image acquiring speed and the irradiation uniformity of the laser excited light can be improved, the stability and reliability of equipment can be improved, the cost can be effectively lowered, the utilization rate of the light source can be increased, and the heat radiation characteristic can be greatly improved.
Description
Technical field
The present invention relates to a kind of optical imaging field, be specifically related to a kind of fluoroscopic imaging device and method.
Background technology
Due to the single spectrum characteristic of laser, the optimal excitation source arrived in the only fluoroscopic examination of the suitable wavelength that LASER Light Source is launched.But, due to LASER Light Source self high directionality and cannot the characteristic of uniform illumination large area sample, current LASER Light Source is only limitted to single-point in the application in fluoroscopic examination field and detects or linearity test.Because single-point detection or linearity test once can only detect a point or a line, this type of detection system must use the motion of high complexity at present, in order to obtain the fluoroscopic image of testing sample two dimensions.Therefore, this type of this type of scanning device is due to the existence of motion structure, and one-piece construction is all very complicated and Installation and Debugging difficulty is very large.Meanwhile, because fluoroscopic image is that the speed of Image Acquisition slowly by synthesizing after a detection or line detection.
Traditional large area non-scanning type fluoroscopic imaging systems uses wide spectral high-intensity light source usually, and such as arc lamp or high strength light emitting diode, as the excitation source of fluorescent samples to be measured.In order to filter out for the necessary narrow-band spectrum of fluorescence excitation from broad spectrum light source, this kind of excitation source needs to coordinate very expensive high-quality optical filter to use, and makes the output light of most broad spectrum light source be colored sheet and filters out.Therefore, the service efficiency of this types of light sources is very low.Use arc lamp as excitation source, also must consider heat dissipation problem in system level.
Summary of the invention
Technical matters to be solved by this invention is to provide a kind of fluoroscopic imaging device and method, the defect such as solve traditional large area non-scanning type fluoroscopic imaging systems complex structure, Installation and Debugging difficulty is large, image acquisition speed is slow, with high costs, light source utilization rate is low, heat dissipation characteristics is poor.
The technical scheme that the present invention solves the problems of the technologies described above is as follows:
According to one aspect of the present invention, provide a kind of fluoroscopic imaging device, comprise at least one group of LASER Light Source, testing sample, optical filter, imaging lens, imageing sensor and intelligent terminal.Described LASER Light Source comprises shaking device and at least even tabula rasa of a slice, wherein said even tabula rasa is arranged on laser array dorsal part and described even tabula rasa side is fixedly connected on shaking device, the uneven spot of irradiation on the microcosmic eliminating LASER Light Source generation itself; Described optical filter to be positioned at above testing sample and to be fixed on the lower end of described imaging lens, and described imageing sensor is fixed on the upper end of imaging lens, and described imageing sensor is connected to intelligent terminal; Described LASER Light Source sends exciting light and to be irradiated on testing sample and to produce fluorescence, described fluorescence is irradiated on optical filter, only have the fluorescence of specific wavelength through optical filter in described fluorescence and expose in imaging lens, imaging lens is collected the fluorescence of specific wavelength and is focused it on imageing sensor, fluorescent optics signal is converted into electric signal by imageing sensor, gather two-dimensional spatial location information simultaneously, form spatial image information and be sent to intelligent terminal, intelligent terminal display space image.
According to another aspect of the present invention, provide a kind of fluorescence imaging method, comprising:
LASER Light Source sends exciting light and to be irradiated on testing sample and to produce fluorescence;
Described fluorescence is irradiated on optical filter, only has the fluorescence of specific wavelength through optical filter and expose in imaging lens in described fluorescence;
Imaging lens is collected the fluorescence of specific wavelength and is focused it on imageing sensor;
Fluorescent optics signal is converted into electric signal by imageing sensor, gathers two-dimensional spatial location information simultaneously, forms spatial image information and is sent to intelligent terminal;
Intelligent terminal display space image.
The invention has the beneficial effects as follows: one of the present invention should imaging device and method, produce fluorescence to carrying out laser excitation in whole testing sample effective coverage simultaneously simultaneously, veiling glare is filtered out by optical filter, make the fluorescence of specific wavelength through optical filter and by imaging lens by imaging on fluorescent foci to imageing sensor, effectively improve the speed of Image Acquisition, the labyrinth of multiple moving component in legacy equipment is enormously simplify by shaking device, improve the light-struck homogeneity of laser excitation, the stability of raising equipment and reliability, effectively reduce cost, improve the utilization factor of light source, substantially improve heat dissipation characteristics.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of fluoroscopic imaging device of the present invention;
Fig. 2 is laser array structure schematic diagram in the embodiment of the present invention;
Fig. 3 is two groups of laser point light source distribution front views in the embodiment of the present invention;
Fig. 4 is the side view of LASER Light Source in the embodiment of the present invention.
In accompanying drawing, the list of parts representated by each label is as follows:
1, LASER Light Source, 2, exciting light, 3, testing sample, 4, fluorescence, 5, optical filter, 6, imaging lens, 7, imageing sensor, 8, intelligent terminal;
11 laser point light sources, the 111, first laser point light source, the 112, second laser spots light, the 121, first even tabula rasa, 122 second even tabula rasas, 13 shaking devices.
Embodiment
Be described principle of the present invention and feature below in conjunction with accompanying drawing, example, only for explaining the present invention, is not intended to limit scope of the present invention.
Embodiment one, a kind of fluoroscopic imaging device, be described in detail a kind of fluoroscopic imaging device that the present embodiment provides below in conjunction with Fig. 1
As shown in Figure 1, a kind of structural representation of fluoroscopic imaging device, comprises at least one group of LASER Light Source 1, testing sample 3, optical filter 5, imaging lens 6, imageing sensor 7 and intelligent terminal 8.Described optical filter 5 is positioned at also just to the lower port of described imaging lens 6 above testing sample 3, and described imageing sensor 7 is fixed on the upper end of imaging lens 6, and described imageing sensor 7 is connected to intelligent terminal 8.There is shown two groups of LASER Light Source 1, described LASER Light Source 1 sends exciting light 2 and to be irradiated on testing sample 3 and to produce fluorescence 4, described fluorescence 4 is irradiated on optical filter 5, only have the fluorescence of specific wavelength through optical filter 5 in described fluorescence 4 and expose in imaging lens 6, imaging lens 6 is collected the fluorescence of specific wavelength and is focused it on imageing sensor 7, fluorescence 4 optical signalling is converted into electric signal by imageing sensor 7, gather two-dimensional spatial location information simultaneously, form spatial image information and be sent to intelligent terminal 8, intelligent terminal 8 display space image.
In the present embodiment, described imageing sensor 7 adopts charge-coupled device (CCD) (CCD) or complementary metal oxide semiconductor (CMOS) (CMOS) imageing sensor.Described imaging lens 6 adopts Large visual angle optical imaging lens, make imaging lens 6 can to sample product to be tested 3 region complete imaging, the combination of imageing sensor 7 and imaging lens 6 makes fluoroscopic imaging device can gather whole fluorescence 4 in testing sample 3 effective coverage simultaneously.Spatial image information is sent to intelligent terminal 8 by wired or wireless mode and shows by imageing sensor 7.
Preferably, described fluoroscopic imaging device comprises even number set LASER Light Source 1 and is symmetrical arranged, and can improve the homogeneity that in effective irradiation area, exciting light 2 irradiates.
As shown in Figure 2, described one group of LASER Light Source 1 comprises by the identical laser point light source 11 of multiple peak wavelength, described multiple laser point light source 11 forms laser array with certain array format, such as: described multiple laser point light source 11 can be arranged in circular array, square array or other form arrays, this array structure can solve two problems: one is from LASER Light Source 1 in effective irradiation distance of testing sample 3, effectively improves the homogeneity of testing sample 3 being carried out to the field of illumination of exciting irradiation; Two be can improve LASER Light Source 1 heat dissipation characteristics thus effective improve its serviceable life.By reducing the power of each single laser point light source 11, the heat that each single laser point light source 11 produces is dispersed to a larger area of space, compares and uses single high power laser sources, effectively improve the problem of heat accumulation.
In addition, described one group of LASER Light Source 1 also can comprise the laser point light source (such as 680 nanometers and 780 nanometers) of two groups of different peak wavelengths, as shown in Figure 3, often organize laser point light source and comprise the identical laser point light source of multiple peak wavelength, described two groups of laser point light source uniform crossover arrayed, can independently control, in order to excite the exciting light of two kinds of different peak wavelengths.After first laser point light source 111 and the second laser point light source 112 irradiate testing sample, testing sample will produce the fluorescence of different wave length.
Preferably, a kind of fluoroscopic imaging device of the present invention comprises the second optical filter and slip and fills 13 and put, described second optical filter and optical filter 5 are disposed side by side on the lower port of imaging lens 6 and two panels optical filter can move around, and the colour filter of described two panels optics is for filtering the veiling glare in two kinds of fluorescence; Described carriage 13 makes optical filter 5 or the second optical filter just to the lower port of imaging lens 6, in order to switch the light path of different wave length for the two panels optical filter that moves around.Certainly, a kind of two waveband narrowband optical color filter also can be selected to substitute optical filter in the present embodiment and the second optical filter, preferably imaging effect can be obtained equally.
Described LASER Light Source 1 comprises shaking device 13 and at least even tabula rasa of a slice, and described vibrating device 13 produces vibrations for making even tabula rasa, eliminates the uneven spot of irradiation on microcosmic that LASER Light Source 1 produces itself.As shown in Figure 4, in the present embodiment, described LASER Light Source comprises the first even tabula rasa 121 and the second even tabula rasa 122, wherein, first even tabula rasa 121 is arranged on the nearer position of distance laser array and its one end is fixedly connected on shaking device 13, and the second even tabula rasa 122 is arranged on distance laser array position far away in order to eliminate the laser facula on microcosmic further.In addition, the frequency of described shaking device 13 can pre-set, and when the set of frequency of shaking device 13 is to enough high, as when the frequency of shaking device reaches 1000Hz in the present embodiment, laser facula can be made in effective lighting region to eliminate completely.
The present invention uses LASER Light Source as the excitation source of fluorescence, because LASER Light Source has stronger unicity, the exciting light of single wavelength can be produced, so the optical filter that wave band can be adopted relatively wide, do not need photochromic color filter even completely, coordinate the use of suitable fluorescence color filter can reach good optical imaging effect, compared to the optical filter needing narrower wave band in legacy equipment excitation light path, greatly reduce the index request to optical filter, significantly reduce cost.Compare to use and be similar to the traditional Large visual angle imaging device of the broad spectrum light source such as arc lamp or LED as excitation source, the present invention greatly can improve the sensitivity of fluoroscopic imaging device.
Embodiment two, a kind of fluorescence imaging method, be described in detail a kind of fluorescence imaging method provided the present embodiment below.
A kind of fluorescence imaging method, comprising:
LASER Light Source 1 sends exciting light 2 and to be irradiated on testing sample 3 and to produce fluorescence 4;
Described fluorescence 4 is irradiated on optical filter 5, only has the fluorescence of specific wavelength through optical filter 5 and expose in imaging lens 6 in described fluorescence 4;
Imaging lens 6 is collected the fluorescence of specific wavelength and is focused it on imageing sensor 7;
Fluorescent optics signal is converted into electric signal by imageing sensor 7, gathers two-dimensional spatial location information simultaneously, forms spatial image information and is sent to intelligent terminal 8;
Intelligent terminal 8 display space image.
The laser point light source 11 that in described one group of LASER Light Source 1, multiple peak wavelength is identical forms laser array with certain array format, effectively improve the homogeneity of testing sample 3 being carried out to the field of illumination of exciting irradiation, the heat dissipation characteristics of LASER Light Source 1 can be improved in addition thus effectively improve its serviceable life.
In the present embodiment, in two groups of laser point light sources in described one group of LASER Light Source 1, the laser point light source 111 or 112 that multiple peak wavelengths often in group laser point light source are identical, described two groups of laser point light sources 111 and 112 uniform crossover arrayed, send the exciting light of two kinds of different wave lengths, slant respectively on testing sample 3 and produce the different fluorescence of two kinds of wavelength, described fluorescence is injected in imaging lens through optical filter 5 or the second optical filter.
In the present embodiment, the second optical filter in described fluoroscopic imaging device and optical filter 5 are disposed side by side on the lower port of imaging lens 6 and two panels optical filter can move around, and described two panels optical filter filters the veiling glare in two kinds of fluorescence; Carriage in the described fluoroscopic imaging device two panels optical filter that is used for moving around makes optical filter or the second optical filter just to imaging lens lower port, in order to switch the light path of different wave length.
Wherein, optical filter 5 and the second optical filter according to the wavelength chooses of fluorescence, can filter the veiling glare in the fluorescence of different wave length respectively, make imaging effect better.Preferably, a kind of two waveband narrowband optical color filter can be selected to substitute optical filter in the present embodiment and the second optical filter, preferably imaging effect can be obtained equally.
In the present embodiment, LASER Light Source comprises the first even tabula rasa 121 and the second even tabula rasa 122, and described first even tabula rasa 121 is arranged on laser array offside, and the second even tabula rasa 122 is arranged, for improving the light-struck homogeneity of laser excitation away from laser array.The side of wherein said first even tabula rasa 121 is fixed on shaking device 13, described shaking device 13 produces vibrations for making even tabula rasa, when the frequency of shaking device is enough high, the uneven spot of irradiation on the microcosmic of LASER Light Source generation itself can be eliminated, when the present embodiment medium frequency reaches 1000Hz, laser facula can be made in effective lighting region to eliminate completely.Certainly, if in order to reach better even light effect, one or more even tabula rasa can be set again along optical path direction.Described intelligent terminal 8 can be the interactive devices such as PC, smart mobile phone, PDA, and described intelligent terminal 8 is by completing data message transmission between wired and/or wireless mode and imageing sensor 7.
In the present invention, the distance between testing sample and imaging lens is unsuitable excessive, all then will effect diagram as image quality.
One of the present invention should imaging device and method, produce fluorescence to carrying out laser excitation in whole testing sample effective coverage simultaneously, veiling glare is filtered out by optical filter, make the fluorescence of specific wavelength through optical filter and by imaging lens by imaging on fluorescent foci to imageing sensor, effectively improve the speed of Image Acquisition, the labyrinth of multiple moving component in legacy equipment is enormously simplify by shaking device, improve the light-struck homogeneity of laser excitation, the stability of raising equipment and reliability, effectively reduce cost, improve the utilization factor of light source, substantially improve heat dissipation characteristics.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. a fluoroscopic imaging device, is characterized in that: comprise at least one group of LASER Light Source, testing sample, optical filter, imaging lens, imageing sensor and intelligent terminal;
Described optical filter is positioned at above testing sample, and just to the lower port of described imaging lens, described imageing sensor is fixed on the upper end of imaging lens, and described imageing sensor is connected to intelligent terminal;
Described LASER Light Source sends exciting light and to be irradiated on testing sample and to produce fluorescence, described fluorescence is irradiated on optical filter, only have the fluorescence of specific wavelength through optical filter in described fluorescence and expose in imaging lens, imaging lens is collected the fluorescence of specific wavelength and is focused it on imageing sensor, fluorescent optics signal is converted into electric signal by imageing sensor, gather two-dimensional spatial location information simultaneously, form spatial image information and be sent to intelligent terminal, intelligent terminal display space image.
2. a kind of fluoroscopic imaging device according to claim 1, is characterized in that: described one group of LASER Light Source comprises by the identical laser point light source of multiple peak wavelength, and described multiple laser point light source arranges formation laser array in the form of an array.
3. a kind of fluoroscopic imaging device according to claim 1, it is characterized in that: described one group of LASER Light Source comprises the laser point light source of two groups of different peak wavelengths, often organize laser point light source and comprise the identical laser point light source of multiple peak wavelength, described two groups of laser point light source uniform crossover arrayed, send the exciting light of two kinds of different wave lengths, be irradiated to respectively on testing sample and produce the different fluorescence of two kinds of wavelength.
4. a kind of fluoroscopic imaging device according to claim 3, it is characterized in that: also comprise the second optical filter and carriage, described second optical filter and optical filter are disposed side by side on the lower port of imaging lens and two panels optical filter can move around, and the colour filter of described two panels optics is for filtering the veiling glare in two kinds of fluorescence; The described carriage two panels optical filter that is used for moving around makes optical filter or the second optical filter just to imaging lens lower port, in order to switch the light path of different wave length.
5. a kind of fluoroscopic imaging device according to any one of Claims 1-4, it is characterized in that: described LASER Light Source also comprises shaking device and at least even tabula rasa of a slice, described vibrating device is used for making even tabula rasa produce vibrations, eliminates the uneven spot of irradiation on the microcosmic of LASER Light Source generation itself; Wherein described in a slice, even tabula rasa is arranged on laser array offside, and its side is fixedly connected on shaking device, and all the other even tabula rasas set gradually away from laser array, for improving the light-struck homogeneity of laser excitation.
6. a fluorescence imaging method, is characterized in that: it adopts a kind of fluoroscopic imaging device described in claim 1-5, comprising:
Described LASER Light Source sends exciting light and to be irradiated on testing sample and to produce fluorescence;
Described fluorescence is irradiated on optical filter, only has the fluorescence of specific wavelength through optical filter and expose in imaging lens in described fluorescence;
Imaging lens is collected the fluorescence of specific wavelength and is focused it on imageing sensor;
Fluorescent optics signal is converted into electric signal by imageing sensor, gathers two-dimensional spatial location information simultaneously, forms spatial image information and is sent to intelligent terminal;
Intelligent terminal display space image.
7. a kind of fluorescence imaging method according to claim 6, is characterized in that: the laser point light source that in described one group of LASER Light Source, multiple peak wavelength is identical arranges formation laser array in the form of an array.
8. a kind of fluorescence imaging method according to claim 6, it is characterized in that: in two groups of laser point light sources in described one group of LASER Light Source, the laser point light source that multiple peak wavelengths often in group laser point light source are identical, described two groups of laser point light source uniform crossover arrayed, send the exciting light of two kinds of different wave lengths, be irradiated to respectively on testing sample and produce the different fluorescence of two kinds of wavelength.
9. a kind of fluorescence imaging method according to claim 8, it is characterized in that: the second optical filter in described fluoroscopic imaging device and optical filter are disposed side by side on the lower port of imaging lens and two panels optical filter can move around, and described two panels optical filter filters the veiling glare in two kinds of fluorescence; Carriage in the described fluoroscopic imaging device two panels optical filter that is used for moving around makes optical filter or the second optical filter just to imaging lens lower port, in order to switch the light path of different wave length.
10. a kind of fluorescence imaging method described in any one of claim 6 to 9, it is characterized in that: the shaking device in described LASER Light Source makes even tabula rasa produce vibrations, eliminate the uneven spot of irradiation on the microcosmic of LASER Light Source generation itself, wherein described in a slice, even tabula rasa is arranged on laser array offside, and its side is fixedly connected on shaking device, all the other even tabula rasas set gradually away from laser array, for improving the light-struck homogeneity of laser excitation.
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| CN105606581A (en) * | 2016-03-21 | 2016-05-25 | 中国工程物理研究院激光聚变研究中心 | Super-continuum spectrum light source based multicolor fluorescence imaging system |
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| CN111487037A (en) * | 2020-04-16 | 2020-08-04 | 中国科学院微电子研究所 | Light source uniformity detection system and method |
| CN112345504A (en) * | 2020-10-30 | 2021-02-09 | 上海世艾生物科技有限公司 | Integrated fluorescence detection device |
| CN112540067A (en) * | 2020-12-04 | 2021-03-23 | 长春理工大学 | Magnetic suspension gene biological imaging microfluidic chip switching device and imaging method thereof |
| CN114686351A (en) * | 2020-12-31 | 2022-07-01 | 上海新微技术研发中心有限公司 | Imaging detection components of PCR nucleic acid detector |
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| CN114686351A (en) * | 2020-12-31 | 2022-07-01 | 上海新微技术研发中心有限公司 | Imaging detection components of PCR nucleic acid detector |
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Application publication date: 20150513 |