CN112294408A - A dual-purpose microfluidic sperm collection tube and control method - Google Patents
A dual-purpose microfluidic sperm collection tube and control method Download PDFInfo
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- CN112294408A CN112294408A CN202010856357.5A CN202010856357A CN112294408A CN 112294408 A CN112294408 A CN 112294408A CN 202010856357 A CN202010856357 A CN 202010856357A CN 112294408 A CN112294408 A CN 112294408A
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- 238000000034 method Methods 0.000 title claims description 16
- 238000005192 partition Methods 0.000 claims abstract description 31
- 239000012528 membrane Substances 0.000 claims abstract description 14
- 238000001514 detection method Methods 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 230000001850 reproductive effect Effects 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 description 6
- 238000011144 upstream manufacturing Methods 0.000 description 6
- 230000002159 abnormal effect Effects 0.000 description 5
- 230000004720 fertilization Effects 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 238000000432 density-gradient centrifugation Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 208000000509 infertility Diseases 0.000 description 3
- 230000036512 infertility Effects 0.000 description 3
- 231100000535 infertility Toxicity 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 208000007466 Male Infertility Diseases 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 208000032170 Congenital Abnormalities Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 239000008119 colloidal silica Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000003642 reactive oxygen metabolite Substances 0.000 description 1
- 229910000077 silane Inorganic materials 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods
- A61B17/42—Gynaecological or obstetrical instruments or methods
- A61B17/425—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation
- A61B17/43—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation for artificial insemination
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Surgery (AREA)
- Engineering & Computer Science (AREA)
- Gynecology & Obstetrics (AREA)
- Biomedical Technology (AREA)
- Pregnancy & Childbirth (AREA)
- Reproductive Health (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical & Material Sciences (AREA)
- Water Supply & Treatment (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Heart & Thoracic Surgery (AREA)
- Medical Informatics (AREA)
- Molecular Biology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
本发明属于生殖医学检测技术领域，公开了一种两用式微流控精子收集管，外壳呈圆柱形，内部中空；外壳底部设置有微孔滤膜，微孔滤膜上侧设置有第一隔板，微孔滤膜与第一隔板之间预留有一定间隙；第一隔板为半圆形，设置于外壳内部一侧；外壳内转动套接有内壳，内壳上端一体化设置有卡板，内壳外壁与外壳内部紧密贴合，内壳横截面积为半圆形，内壳底部一体化设置有第二隔板。第二隔板设置于第一隔板上侧，第二隔板与第一隔板紧密贴合。内壳外壁与所述外壳内部紧密贴合。本发明结构简单合理，可快速完成精子的收集工作，提高了工作者的工作效率，降低了劳动强度。
The invention belongs to the technical field of reproductive medicine detection, and discloses a dual-purpose microfluidic sperm collection tube. There is a certain gap reserved between the microporous filter membrane and the first partition; the first partition is semicircular and set on one side inside the outer shell; the inner shell is rotatably connected to the inner shell, and the upper end of the inner shell is integrated There is a clamping plate, the outer wall of the inner shell is closely attached to the inside of the outer shell, the cross-sectional area of the inner shell is semicircular, and the bottom of the inner shell is integrally provided with a second partition. The second partition is arranged on the upper side of the first partition, and the second partition is closely attached to the first partition. The outer wall of the inner shell is in close contact with the inside of the outer shell. The invention has a simple and reasonable structure, can quickly complete the sperm collection work, improves the work efficiency of workers, and reduces labor intensity.
Description
Technical Field
The invention belongs to the technical field of reproductive medicine detection, and particularly relates to a dual-purpose microfluidic sperm collecting pipe and a control method.
Background
Currently, according to the statistics of the world health organization, infertility becomes the third world-wide disease after tumors and cardiovascular and cerebrovascular diseases. Chinese fertile couples are about 2.3 million people, of which there are nearly ten million infertility patients, and increase in speed by hundreds of thousands each year. In recent years, the incidence of male infertility has been on the rise year by year due to environmental pollution, pressure of life, and changes in lifestyle. Assisted reproduction technologies such as intrauterine artificial insemination (IUI), in vitro fertilization-embryo transfer (IVF-ET), intracytoplasmic sperm microinjection (ICSI) and the like are the main technical methods for treating infertility at present. The advent and widespread use of assisted reproduction technology has enabled more and more male infertility to be effectively treated, but the success rate remains low and needs to be further refined. The collection of good quality sperm with sufficient quantity, good vitality, normal shape and complete DNA is the first technical challenge, and the use of good quality sperm in assisted reproduction technology can avoid the birth of test-tube infants with fertilization failure, low fertilization and even congenital defects to a certain extent.
Currently, the methods for collecting sperm, which are widely used clinically, are the upstream method and the density gradient centrifugation method, but both methods have certain limitations, for example, the upstream method cannot distinguish sperm with normal morphology from sperm with abnormal morphology, which may cause a large amount of sperm with normal motility and abnormal morphology to be mixed into the finally collected sperm, while the gradient liquid used in the density gradient centrifugation method is mostly colloidal silica particles coated by silane, and repeated centrifugation steps may cause generation of active oxygen, which may greatly damage DNA of sperm with normal appearance and normal morphology, thereby affecting sperm quality.
Through the above analysis, the problems and defects of the prior art are as follows:
(1) the upstream method cannot distinguish the sperm with normal morphology from the sperm with abnormal morphology, which can cause a large amount of the sperm with normal activity but abnormal morphology to be mixed into the finally collected sperm, thereby causing low fertilization rate;
(2) after the upstream is finished, the sperm suspension with better vitality on the upper layer needs to be sucked out manually, but if the operation is not proper, the sperms and impurities with poor vitality on the lower layer are easy to be sucked in;
(3) repeated centrifugation steps in density gradient centrifugation can result in the production of reactive oxygen species that can greatly impair the DNA integrity of otherwise viable and morphologically normal sperm, thereby affecting sperm quality, resulting in poor fertilization rates or abnormal embryo development.
The difficulty in solving the above problems and defects is:
(1) sorting out sperms with good vitality and normal shape in the upstream sperms;
(2) how to avoid absorbing sperms and impurities with poor activity in the lower layer;
(3) how to avoid using chemical substances such as gradient liquid and the like and generating active oxygen to damage sperms by repeated centrifugation.
The significance of solving the problems and the defects is as follows: developing a new sperm optimization method suitable for the assisted reproduction technology, sorting high-quality sperm with good vitality, normal shape and complete DNA, and improving the outcome of the assisted reproduction technology.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a dual-purpose microfluidic sperm collecting tube and a control method.
The invention is realized in such a way that a dual-purpose microfluidic sperm collecting tube is provided with:
a housing;
the shell is cylindrical and hollow inside;
a microporous filter membrane is arranged at the bottom of the shell, a first partition plate is arranged on the upper side of the microporous filter membrane, and a certain gap is reserved between the microporous filter membrane and the first partition plate; the first partition plate is semicircular and is arranged on one side in the shell;
the inner shell is rotatably sleeved in the outer shell, the cross section area of the inner shell is semicircular, and a second partition plate is integrally arranged at the bottom of the inner shell.
Further, the second partition plate is arranged on the upper side of the first partition plate, and the second partition plate is tightly attached to the first partition plate.
Further, the outer wall of the inner shell is tightly attached to the inside of the outer shell.
Furthermore, a clamping plate is integrally arranged on the outer side of the upper end of the inner shell.
By combining all the technical schemes, the invention has the advantages and positive effects that: before collection, the inner shell is rotated to one side of the outer shell, the sperms flow upstream through self activity, and the aperture of the microporous filter membrane (3-5 microns) is slightly larger than the width of the head of the normal sperms (2.5-3.2 microns), so that the sperms with normal shapes can pass through. After a certain time of collection, the inner shell is rotated back to the original position, the bottom is closed, and the tube is directly taken out, namely the collected high-quality sperm suspension is obtained in the tube, and the sperm suspension does not need to be sucked out manually. The invention has simple and reasonable structure, can quickly finish the collection work of high-quality sperms, is beneficial to improving the fertilization rate of the auxiliary reproduction technology, and can improve the working efficiency of workers and reduce the labor intensity.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a dual-purpose microfluidic sperm collection tube according to an embodiment of the present invention;
FIG. 2 is a schematic structural diagram of a dual-purpose microfluidic sperm collection tube according to an embodiment of the present invention;
in the figure: 1. a housing; 2. clamping a plate; 3. a first separator; 4. a microporous filtration membrane; 5. a second separator; 6. an inner shell.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a dual-purpose microfluidic sperm collecting tube and a control method thereof, and the invention is described in detail with reference to the attached drawings.
As shown in fig. 1 to 2, the dual-purpose microfluidic sperm collection tube provided by the present invention comprises: the device comprises an outer shell 1, a clamping plate 2, a first partition plate 3, a microporous filter membrane 4, a second partition plate 5 and an inner shell 6.
The shell 1 is cylindrical and hollow inside; a microporous filter membrane 4 is arranged at the bottom of the shell 1, a first partition plate 3 is arranged at the upper side of the microporous filter membrane 4, and a certain gap is reserved between the microporous filter membrane 4 and the first partition plate 3; the first clapboard 3 is semicircular and is arranged on one side in the shell 1; inner shell 6 has been cup jointed to 1 internal rotation of shell, and 6 upper end integrations of inner shell are provided with cardboard 2, and 6 outer walls of inner shell closely laminate with 1 inside of shell, and 6 cross-sectional areas of inner shell are semi-circular, and 6 bottoms integrations of inner shell are provided with second baffle 5.
The second partition board 5 is arranged on the upper side of the first partition board 3, and the second partition board 5 is tightly attached to the first partition board 3.
When the device is used, the outer shell 1 is vertically placed in a centrifugal tube, the centrifugal tube can be clamped on the centrifugal tube through the clamping plate 2, the inner shell 6 is rotated through the clamping plate 2, the second partition plate 5 is overlapped with the first partition plate 3, sperms enter the outer shell 1 through the microporous filter membrane 4, the inner shell 6 is rotated through the clamping plate 2 again, the inner shell 6 is reset, and the collection can be completed after the sperms are taken out.
In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, should not be construed as limiting the invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
The above description is only for the purpose of illustrating the present invention and the appended claims are not to be construed as limiting the scope of the invention, which is intended to cover all modifications, equivalents and improvements that are within the spirit and scope of the invention as defined by the appended claims.
Claims (5)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010856357.5A CN112294408A (en) | 2020-08-24 | 2020-08-24 | A dual-purpose microfluidic sperm collection tube and control method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010856357.5A CN112294408A (en) | 2020-08-24 | 2020-08-24 | A dual-purpose microfluidic sperm collection tube and control method |
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| Publication Number | Publication Date |
|---|---|
| CN112294408A true CN112294408A (en) | 2021-02-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010856357.5A Pending CN112294408A (en) | 2020-08-24 | 2020-08-24 | A dual-purpose microfluidic sperm collection tube and control method |
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| Country | Link |
|---|---|
| CN (1) | CN112294408A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5575914A (en) * | 1994-12-23 | 1996-11-19 | Vance Products Incorporated | Sperm filter trap having compressed glass wool filter material |
| US20080311653A1 (en) * | 2007-03-14 | 2008-12-18 | Farhang Abed | Method and apparatus for regulating optimum flow of semen and separating motile sperms |
| WO2012126478A1 (en) * | 2011-03-21 | 2012-09-27 | Motilitycount Aps | Device for analysis of cellular motility |
| CN104099293A (en) * | 2014-06-30 | 2014-10-15 | 浙江星博生物科技有限公司 | Sperm optimal selection method based on microporous membrane |
| CN205756869U (en) * | 2016-06-27 | 2016-12-07 | 广西壮族自治区妇幼保健院 | A kind of trace sperm cryopreservation device with centrifugal function |
| CN107460116A (en) * | 2017-08-31 | 2017-12-12 | 同济大学苏州研究院 | One kind is used for the preferable sperm swim-up device of sperm |
| CN110079436A (en) * | 2019-05-31 | 2019-08-02 | 柳州市妇幼保健院 | A kind of sperm size preferred embodiment and its application method |
| CN213156327U (en) * | 2020-08-24 | 2021-05-11 | 成都西囡妇科医院有限公司 | Dual-purpose microfluidic sperm collecting pipe |
-
2020
- 2020-08-24 CN CN202010856357.5A patent/CN112294408A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5575914A (en) * | 1994-12-23 | 1996-11-19 | Vance Products Incorporated | Sperm filter trap having compressed glass wool filter material |
| US20080311653A1 (en) * | 2007-03-14 | 2008-12-18 | Farhang Abed | Method and apparatus for regulating optimum flow of semen and separating motile sperms |
| WO2012126478A1 (en) * | 2011-03-21 | 2012-09-27 | Motilitycount Aps | Device for analysis of cellular motility |
| CN104099293A (en) * | 2014-06-30 | 2014-10-15 | 浙江星博生物科技有限公司 | Sperm optimal selection method based on microporous membrane |
| CN205756869U (en) * | 2016-06-27 | 2016-12-07 | 广西壮族自治区妇幼保健院 | A kind of trace sperm cryopreservation device with centrifugal function |
| CN107460116A (en) * | 2017-08-31 | 2017-12-12 | 同济大学苏州研究院 | One kind is used for the preferable sperm swim-up device of sperm |
| CN110079436A (en) * | 2019-05-31 | 2019-08-02 | 柳州市妇幼保健院 | A kind of sperm size preferred embodiment and its application method |
| CN213156327U (en) * | 2020-08-24 | 2021-05-11 | 成都西囡妇科医院有限公司 | Dual-purpose microfluidic sperm collecting pipe |
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