CN104099293A - Sperm optimal selection method based on microporous membrane - Google Patents
Sperm optimal selection method based on microporous membrane Download PDFInfo
- Publication number
- CN104099293A CN104099293A CN201410304746.1A CN201410304746A CN104099293A CN 104099293 A CN104099293 A CN 104099293A CN 201410304746 A CN201410304746 A CN 201410304746A CN 104099293 A CN104099293 A CN 104099293A
- Authority
- CN
- China
- Prior art keywords
- sperm
- concentration
- microporous membrane
- nutrient solution
- method based
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000012982 microporous membrane Substances 0.000 title claims abstract description 28
- 238000010187 selection method Methods 0.000 title abstract 3
- 235000015097 nutrients Nutrition 0.000 claims abstract description 20
- 238000000034 method Methods 0.000 claims abstract description 12
- 210000000582 semen Anatomy 0.000 claims description 7
- 239000011734 sodium Substances 0.000 claims description 6
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 claims description 4
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 4
- 229960003531 phenolsulfonphthalein Drugs 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 3
- 229910019142 PO4 Inorganic materials 0.000 claims description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 3
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 3
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 3
- LLSDKQJKOVVTOJ-UHFFFAOYSA-L calcium chloride dihydrate Chemical compound O.O.[Cl-].[Cl-].[Ca+2] LLSDKQJKOVVTOJ-UHFFFAOYSA-L 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 3
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 3
- 239000010452 phosphate Substances 0.000 claims description 3
- 239000011591 potassium Substances 0.000 claims description 3
- 229910052700 potassium Inorganic materials 0.000 claims description 3
- 229910052708 sodium Inorganic materials 0.000 claims description 3
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 2
- NGSFWBMYFKHRBD-UHFFFAOYSA-N sodium;2-hydroxypropanoic acid Chemical compound [Na+].CC(O)C(O)=O NGSFWBMYFKHRBD-UHFFFAOYSA-N 0.000 claims description 2
- 238000012216 screening Methods 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 10
- 238000011084 recovery Methods 0.000 abstract description 6
- 230000005484 gravity Effects 0.000 abstract description 3
- 230000000903 blocking effect Effects 0.000 abstract 1
- 230000002860 competitive effect Effects 0.000 abstract 1
- 231100000688 decreased sperm quality Toxicity 0.000 abstract 1
- 238000003908 quality control method Methods 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 description 7
- 238000013016 damping Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 238000011144 upstream manufacturing Methods 0.000 description 4
- 210000003756 cervix mucus Anatomy 0.000 description 2
- 238000000432 density-gradient centrifugation Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000004899 motility Effects 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000009328 Perro Species 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000000469 anti-sperm effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 239000002520 smart material Substances 0.000 description 1
- 230000009233 sperm competition Effects 0.000 description 1
- 230000019100 sperm motility Effects 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a sperm optimal selection method based on a microporous membrane. High quality and high activity sperms preferentially reach a sperm nutrient solution by using the initiative attracting of the sperm nutrient solution to sperms, the blocking of the microporous membrane, and microporous competitive passing, and under effects of gravity and activity competition, the sperms in the sperm nutrient solution are highly energetic. The method provided by the invention is simple in selection, the sperm recovering rate is high, sperm quality reduction is avoided, and the screening effect is good. Therefore, the sperm optimal selection method based on the microporous membrane has the advantages of simple selection, good selection effect, high recovery ratio, easiness in sperm quality control.
Description
Technical field
The present invention relates to sperm preferred method, be specifically related to a kind of sperm preferred method based on microporous membrane.
Background technology
Motility of sperm is one of key factor of becoming pregnant, the seminal fluid that sperm motility rate is high, and the sperm quantity that its motility is strong is relatively high, and the probability of therefore becoming pregnant is also high.The sperm optimization technique extensively adopting at present has the optimization techniques such as upstream technology, density gradient centrifugation.Upstream technology be utilize sperm own vitality upwards swimming principle and design, sperm is in upstream process, can depart from refining some and endogenicly press down smart material, as antisperm antibody, various types of cells etc., and make sperm in vitro the activity time extended, but upstream technology is relatively low to the high quality sperm rate of recovery.Density gradient centrifugation is processed the rear high quality sperm rate of recovery and is improved, but easily causes sperm DNA peroxide injury, and sperm quality declines.Chinese patent notification number is the patent of invention of CN102352305, the micro-fluidic core technology of utilizing is disclosed, detect and screening sperm, its principle is to utilize slightly poor sperm and the white corpuscle of cervical mucus absorption vigor in sample pool, epithelial cell etc., in recycling damping fluid addition pool, add damping fluid, flow high-precision the vigor in sample pool subband in sample collection pond by damping fluid liquid, reach screening and the collection of high motile sperm, but this patent of invention technology is more complicated, should cervical mucus adsorb, damping fluid is passive is again flushed to sample collection pond by sperm stream, sperm is not active Flow, sperm quality quality is wayward.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of screening simply, and screening effect is good, and the rate of recovery is higher, the manageable sperm preferred method based on microporous membrane of sperm quality.
The present invention solves the problems of the technologies described above adopted technical scheme: a kind of sperm preferred method based on microporous membrane, and its step is as follows:
A, seminal fluid is moved in test tube, be covered with the microporous membrane of micropore in the surface coverage of seminal fluid, described microporous membrane is thick is 50～200 microns, and the aperture of described micropore is 10～200 microns;
B, on microporous membrane, add sperm nutrient solution, in described sperm nutrient solution, contain the sodium-chlor that concentration is 0.1mol/L, concentration is the potassium primary phosphate of 0.4mmol/L, concentration is the glucose of 2.7mmol/L, concentration is the Repone K of 4.7mmol/L, concentration is the magnesium sulfate heptahydrate of 0.2mmol/L, concentration is the Calcium dichloride dihydrate of 2mmol/L, concentration is N-2-hydroxyethyl piperazine-N-2-ethyl sulfonic acid disodium salt (Hepes-Na) of 0.02mol/L, concentration is the sodium bicarbonate of 4mmol/L, concentration is 0.3mmol/L Sodium.alpha.-ketopropionate, concentration expressed in percentage by volume is 0.34% Sodium.alpha.-hydroxypropionate (C
3h
5o
3na) phenol red (the Phenol Red) that, mass percentage concentration is 0.002%, this sperm nutrient solution has the effect that initiatively attracts sperm to move, and by the obstruct of microporous membrane, the sperm precedence of high vigor is entered in sperm nutrient solution from micropore,
C, test tube is placed at 37 DEG C, constant temperature 15～45 minutes, reclaims the sperm nutrient solution of microporous membrane top, obtains preferred sperm.
Compared with prior art, the invention has the advantages that a kind of sperm preferred method based on microporous membrane, utilize sperm nutrient solution to attract the active of sperm, the obstruct of microporous membrane, micropore is emulative to be passed through, making the sperm precedence of high quality, high vigor arrive sperm nutrient solution, under gravity and vigor competition, is substantially all the sperm of high vigor in sperm nutrient solution.Method screening of the present invention is simple, and the sperm rate of recovery is higher, avoids sperm quality to decline simultaneously, and screening effect is good, and therefore the present invention is that a kind of screening is simple, and screening effect is good, and the rate of recovery is higher, the manageable sperm preferred method based on microporous membrane of sperm quality.
Brief description of the drawings
Fig. 1 is the schematic diagram of sperm preferred method of the present invention.
Embodiment
Below in conjunction with accompanying drawing, embodiment is described in further detail the present invention.
Embodiment 1
A kind of sperm preferred method based on microporous membrane as shown in the figure, seminal fluid is moved in test tube 1, in the surface coverage of seminal fluid 3, be covered with the microporous membrane 2 of micropore 21, microporous membrane 2 slightly larger in diameter are in the internal diameter of test tube 1, reach all standing, prevent that sperm is from edge flowing, microporous membrane 2 is thick is 50～200 microns, the aperture of micropore 21 is 10～200 microns, determine according to screening sperm size in the aperture of micropore, the maximum diameter that is slightly larger than screening sperm, while screening as people's sperm, the aperture of micropore is 40 microns of left and right; On microporous membrane 2, add sperm nutrient solution 4, in this sperm nutrient solution, containing the sodium-chlor that concentration is 0.1mol/L, the potassium primary phosphate that concentration is 0.4mmol/L, the glucose that concentration is 2.7mmol/L, the Repone K that concentration is 4.7mmol/L, the magnesium sulfate heptahydrate that concentration is 0.2mmol/L, the Calcium dichloride dihydrate that concentration is 2mmol/L, the Hepes-Na that concentration is 0.02mol/L, sodium bicarbonate, the concentration that concentration is 4mmol/L is that 0.3mmol/L Sodium.alpha.-ketopropionate, concentration expressed in percentage by volume are the C of 0.34% (v/v)
3h
5o
3the Phenol Red that Na, mass percentage concentration are 0.002%; Test tube is placed at 37 DEG C, constant temperature 15～45 minutes, sperm initiatively upwards moves to sperm nutrient solution like this, in the double competition of the vigor of gravity, the sperm that vigor is high enters the sperm nutrient solution on upper strata by micropore, the sperm competition power that vigor is low is inadequate, have to, in lower floor, control constant temperature time, just can control the sperm quality in sperm nutrient solution, the sperm nutrient solution that reclaims microporous membrane top, is placed in another test tube, obtains preferred sperm.Be conducive to the operations such as follow-up artificial insemination, in vitro fertilization, Microfertilization.
The material of above-mentioned microporous membrane can be avirulent polypropylene, glass, vegetable fibre etc.; Sperm source can be Mammals, as people, mouse, rat, rabbit, sheep, dog, cat, ox etc., can be also other animal, as fish, two class etc. of dwelling.
Claims (1)
1. the sperm preferred method based on microporous membrane, is characterized in that step is as follows:
A, seminal fluid is moved in test tube, be covered with the microporous membrane of micropore in the surface coverage of seminal fluid, described microporous membrane is thick is 50～200 microns, and the aperture of described micropore is 10～200 microns;
B, on microporous membrane, add sperm nutrient solution, in described sperm nutrient solution, contain the sodium-chlor that concentration is 0.1mol/L, concentration is the potassium primary phosphate of 0.4mmol/L, concentration is the glucose of 2.7mmol/L, concentration is the Repone K of 4.7mmol/L, concentration is the magnesium sulfate heptahydrate of 0.2mmol/L, concentration is the Calcium dichloride dihydrate of 2mmol/L, concentration is N-2-hydroxyethyl piperazine-N-2-ethyl sulfonic acid disodium salt of 0.02mol/L, concentration is the sodium bicarbonate of 4mmol/L, concentration is 0.3mmol/L Sodium.alpha.-ketopropionate, concentration expressed in percentage by volume is 0.34% Sodium.alpha.-hydroxypropionate, mass percentage concentration be 0.002% phenol red,
C, test tube is placed at 37 DEG C, constant temperature 15～45 minutes, reclaims the sperm nutrient solution of microporous membrane top, obtains preferred sperm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410304746.1A CN104099293A (en) | 2014-06-30 | 2014-06-30 | Sperm optimal selection method based on microporous membrane |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410304746.1A CN104099293A (en) | 2014-06-30 | 2014-06-30 | Sperm optimal selection method based on microporous membrane |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104099293A true CN104099293A (en) | 2014-10-15 |
Family
ID=51667835
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410304746.1A Pending CN104099293A (en) | 2014-06-30 | 2014-06-30 | Sperm optimal selection method based on microporous membrane |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104099293A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498346A (en) * | 2014-12-12 | 2015-04-08 | 张群 | Micron-hole-based apparatus for rapidly screening and collecting healthy sperms and application method of micron-hole-based apparatus |
| CN105838591A (en) * | 2016-04-11 | 2016-08-10 | 同济大学苏州研究院 | Sperm selection device and selection method based on principle of fly trap |
| CN106896051A (en) * | 2017-03-06 | 2017-06-27 | 浙江星博生物科技股份有限公司 | A kind of method of human sperm's acrosome reaction ability detection |
| CN112294408A (en) * | 2020-08-24 | 2021-02-02 | 成都西囡妇科医院有限公司 | A dual-purpose microfluidic sperm collection tube and control method |
| US11352597B2 (en) | 2015-09-28 | 2022-06-07 | My123Baby Medical Limited | Medical device for the selective separation of a biological sample |
| CN115701450A (en) * | 2021-08-02 | 2023-02-10 | 邦睿生技股份有限公司 | Sperm sorting device and method |
| US11709122B2 (en) | 2013-11-20 | 2023-07-25 | The Brigham And Women's Hospital, Inc. | System and method for sperm sorting |
| WO2024086456A1 (en) * | 2022-10-21 | 2024-04-25 | Texas Tech University System | Apparatus and method for collection of sperm samples |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012126478A1 (en) * | 2011-03-21 | 2012-09-27 | Motilitycount Aps | Device for analysis of cellular motility |
| CN103335933A (en) * | 2013-06-28 | 2013-10-02 | 浙江星博生物科技有限公司 | Flow cytometry-based sperm acrosome reaction detection reagent |
-
2014
- 2014-06-30 CN CN201410304746.1A patent/CN104099293A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012126478A1 (en) * | 2011-03-21 | 2012-09-27 | Motilitycount Aps | Device for analysis of cellular motility |
| CN103335933A (en) * | 2013-06-28 | 2013-10-02 | 浙江星博生物科技有限公司 | Flow cytometry-based sperm acrosome reaction detection reagent |
Non-Patent Citations (1)
| Title |
|---|
| 王利红等: "改良上游法在体外精液处理中的应用", 《山东医药》 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11709122B2 (en) | 2013-11-20 | 2023-07-25 | The Brigham And Women's Hospital, Inc. | System and method for sperm sorting |
| US11788943B2 (en) | 2013-11-20 | 2023-10-17 | The Brigham And Women's Hospital, Inc. | System and method for sperm sorting |
| US11841309B2 (en) | 2013-11-20 | 2023-12-12 | The Brigham And Women's Hospital, Inc. | System and method for sperm sorting |
| US11946846B2 (en) | 2013-11-20 | 2024-04-02 | The Brigham And Women's Hospital, Inc. | System and method for sperm sorting |
| US11982610B2 (en) | 2013-11-20 | 2024-05-14 | The Brigham And Women's Hospital, Inc. | System and method for sperm sorting |
| CN104498346A (en) * | 2014-12-12 | 2015-04-08 | 张群 | Micron-hole-based apparatus for rapidly screening and collecting healthy sperms and application method of micron-hole-based apparatus |
| US11352597B2 (en) | 2015-09-28 | 2022-06-07 | My123Baby Medical Limited | Medical device for the selective separation of a biological sample |
| CN105838591A (en) * | 2016-04-11 | 2016-08-10 | 同济大学苏州研究院 | Sperm selection device and selection method based on principle of fly trap |
| CN106896051A (en) * | 2017-03-06 | 2017-06-27 | 浙江星博生物科技股份有限公司 | A kind of method of human sperm's acrosome reaction ability detection |
| CN112294408A (en) * | 2020-08-24 | 2021-02-02 | 成都西囡妇科医院有限公司 | A dual-purpose microfluidic sperm collection tube and control method |
| CN115701450A (en) * | 2021-08-02 | 2023-02-10 | 邦睿生技股份有限公司 | Sperm sorting device and method |
| WO2024086456A1 (en) * | 2022-10-21 | 2024-04-25 | Texas Tech University System | Apparatus and method for collection of sperm samples |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104099293A (en) | Sperm optimal selection method based on microporous membrane | |
| Nagata et al. | Live births from artificial insemination of microfluidic-sorted bovine spermatozoa characterized by trajectories correlated with fertility | |
| Morisawa et al. | Effects of osmolality and potassium on motility of spermatozoa from freshwater cyprinid fishes | |
| Leung et al. | Simulating nature in sperm selection for assisted reproduction | |
| Christiaen et al. | Isolation of sea squirt (Ciona) gametes, fertilization, dechorionation, and development | |
| AU2016231560B2 (en) | High efficiency methods of sex sorting sperm | |
| CN104099237A (en) | Sperm optimal selection device based on microtubule passages and optimal selection method | |
| Ahmadkhani et al. | The influence of the female reproductive tract and sperm features on the design of microfluidic sperm-sorting devices | |
| CN102250836A (en) | Two-layer percoll density gradient centrifugal separation method of boar sperms | |
| CN115491301A (en) | Microfluidic chip integrating sperm capacitation, sperm sorting, in-vitro fertilization and embryo culture, and preparation method and application thereof | |
| CN104099236B (en) | A kind of preferred slide of sperm based on micro-pipe fluidway plate and method for optimizing | |
| CN1284849C (en) | Method for separating XY germ cell of mammal | |
| Ma et al. | Advances in microfluidic technology for sperm screening and in vitro fertilization | |
| CN207908182U (en) | A kind of sampling apparatus for detaching zooplankter and phytoplankton | |
| CN208844105U (en) | A kind of micro flow control chip device for Embryo Culture | |
| CN206324012U (en) | A kind of Daphnia magna chronic toxicity test automatic water-replacing and separator | |
| Nicolson et al. | Salt gland function in the green sea turtle Chelonia mydas | |
| Herbicht et al. | Evaluation of a novel microfluidic chip-like device for purifying bovine frozen-thawed semen for in vitro fertilization | |
| Han et al. | Effect of relaxin on in vitro fertilization of porcine oocytes | |
| CN102978196B (en) | Method for extracting total RNA (Ribose Nucleic Acid) and total protein of rotifer | |
| CN102068429B (en) | Application of fadrozole in inducing transformation of differentiated ovary of tilapia mossambica into functional testis and induction method thereof | |
| CN104560866A (en) | Method for in-vitro sperm capacitation and detection of Kunming dog | |
| CN106771029A (en) | One kind is used for Daphnia magna acute toxicity detection kit | |
| CN107058215A (en) | A kind of Pig Oocytes-in Fertilization in vitro liquid and cultural method for adding traditional Chinese medicine monomer | |
| CN208586284U (en) | Preferred sperm cryopreservation slides |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 315040 Zhejiang high tech District of Ningbo City Xinhui Road No. 139 Building No. 2 Applicant after: Chen Binglai Address before: 315040 Zhejiang high tech District of Ningbo City Xinhui Road No. 139 Building No. 2 Applicant before: Zhejiang Cellpro Biotech Co., Ltd. |
|
| COR | Change of bibliographic data | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20141015 |