CN112156063A - Repair essence containing plant stem cell extract and preparation method thereof - Google Patents
Repair essence containing plant stem cell extract and preparation method thereof Download PDFInfo
- Publication number
- CN112156063A CN112156063A CN202011053895.7A CN202011053895A CN112156063A CN 112156063 A CN112156063 A CN 112156063A CN 202011053895 A CN202011053895 A CN 202011053895A CN 112156063 A CN112156063 A CN 112156063A
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- Prior art keywords
- parts
- stem cell
- essence
- gum
- repair
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Abstract
The invention relates to the technical field of essence, and particularly relates to a repair essence containing a plant stem cell extract and a preparation method thereof, wherein the repair essence comprises the following components in parts by weight: 70-80 parts of glycerol aqueous solution, 24-31 parts of bioglycan, 14-19 parts of vegetable gum, 12-16 parts of fibronectin, 9-11 parts of tetrahydro-methyl pyrimidine carboxylic acid, 5-8 parts of 4D hyaluronic acid, 4-6 parts of carboxymethyl chitosan, 3-6 parts of lecithin, 2-3 parts of hydrolyzed algae extract and 0.4-0.8 part of triethanolamine; according to the invention, through the biological carbohydrate gum and the plant gum components arranged in the repair essence, the powdery particles of actinidine and apple polyphenol in the plant gum are suspended in the essence, so that the plant gum is spread on the skin in the massage process in the use of the repair essence, and after the skin epidermal cells are repaired by the essence, the actinidine and the apple polyphenol in the plant gum smoothly permeate into the interior of the epidermis, so that the absorption effect of the skin on the functional components is promoted, and the use effect of the repair essence containing the plant stem cell extract is improved.
Description
Technical Field
The invention relates to the technical field of essence, and particularly relates to repair essence containing a plant stem cell extract and a preparation method thereof.
Background
The repair essence is a high-concentration and high-functionality maintenance product, generally contains more active ingredients, has small molecules and strong permeability, and can be used for deeply repairing different skin tissue problems; the core component is an active matter which has an effect on skin, and the active matter added in the product generally has the cosmetic water content of more than 10 percent, namely the active matter is called essence.
The metabolic state of the keratinocytes on the surface layer of the skin is related to the direct visual effect of the skin, and in the process that the epidermal cells are finally transformed into cutin and are removed, the absorption effect of the cells on external active matters is gradually reduced, so that the effective active matters in the repair essence can only enter the basal layer cells in the epidermis to play the corresponding repair effect on the skin.
Some technical solutions related to a repair essence containing a plant stem cell extract also appear in the prior art, for example, a chinese patent with application number 2019113591271 discloses a fermentation product compounded plant extract anti-aging repair composition, an application thereof, and an essence emulsion containing the composition, wherein the anti-aging repair composition comprises a fermentation product extract and a plant extract in parts by weight, and the fermentation product extract comprises: 20-120 parts of yeast/grape fermentation product extract, 45-180 parts of secondary fission yeast fermentation product filtrate/lysate/extract and 30-160 parts of alteromonas fermentation product extract; the plant extract comprises: 2-20 parts of perilla leaf extract, 3-16 parts of lantana leaf extract, 2-14 parts of bidens pilosa extract, 5-40 parts of aesculus hippocastanum extract, 5-20 parts of thyme extract, 3-36 parts of aloe vera extract, 3-34 parts of centella asiatica extract and 2-12 parts of silybum marianum extract, wherein the yeast extract composition in the technical scheme is compounded with a plant extract composition with strong antioxidant capacity, so that the yeast extract composition provides rich active nutrition for skin, repairs injuries and twists off the early senescence and has extremely strong wound healing effect; however, the technical scheme does not solve the problem that the functional components in the repair essence are permeated into epidermal cells inside the skin, so that the improvement effect of the repair essence on the facial skin is weakened.
In view of the above, in order to overcome the above technical problems, the present invention provides a repair essence containing a plant stem cell extract and a preparation method thereof, and a special repair essence and a preparation method thereof are adopted to solve the above technical problems.
Disclosure of Invention
In order to make up the defects of the prior art, the invention provides the repair essence containing the plant stem cell extract and the preparation method thereof, the powdered particles of the actinidine and the apple polyphenol in the plant gum are suspended in the essence through the biological carbohydrate gum and the plant gum components arranged in the repair essence, so that the plant gum is spread on the skin in the massage process in the use of the repair essence, after the skin epidermal cells are repaired by the essence, the actinidine and the apple polyphenol in the plant gum smoothly permeate into the inside of the epidermis, the absorption effect of the skin on the functional components is promoted, and the use effect of the repair essence containing the plant stem cell extract is improved.
The invention relates to a repair essence containing a plant stem cell extract, which comprises the following components in parts by weight: 70-80 parts of glycerol aqueous solution, 24-31 parts of bioglycan, 14-19 parts of vegetable gum, 12-16 parts of fibronectin, 9-11 parts of tetrahydro-methyl pyrimidine carboxylic acid, 5-8 parts of 4D hyaluronic acid, 4-6 parts of carboxymethyl chitosan, 3-6 parts of lecithin, 2-3 parts of hydrolyzed algae extract and 0.4-0.8 part of triethanolamine; wherein the vegetable gum comprises a parsley stem cell extract, actinidine, apple polyphenol and bioglycan, and the specific gravity of the three is 2:3: 8; the actinidine and the apple polyphenol are powdery particles, and a solid wrapping layer formed by biogasose is arranged on the outer surface of the particles; the biological carbohydrate gum in the glycerol aqueous solution is in a saturated dissolved state, so that the vegetable gum and the biological carbohydrate gum form a suspension colloid in the essence; when the repair essence is used, functional components in the repair essence need to be ensured to enter basal layer cells through gaps of skin epidermis so as to achieve the repair effect on the functions of the skin cells, so that the basal layer cells are gradually converted into cells with a spinous layer, a granular layer and a cuticle in skin metabolism, and then are finally converted into cutin to be removed in the metabolic cycle of the skin, and as the metabolic state of the cutin cells on the surface layer of the skin is related to the direct visual effect of the skin, and in the process that the epidermal cells are finally converted into the cutin to be removed, the absorption effect of the cells on external active substances is gradually reduced, so that the functional active substances in the repair essence can exert the repair effect corresponding to the skin only when entering the basal layer cells in the epidermis; therefore, the invention supplies water needed by keratinocytes in the epidermis by arranging the 4D hyaluronic acid, lecithin and the hydrolyzed algae extract, the barrier function between the granular layer and acanthon stratum cells is recovered, the smoothness of intercellular spaces is recovered, the glycerol and the biological carbohydrate gum regulate the viscosity performance of the essence, and the glycerol and the biological carbohydrate gum are matched with the biological carbohydrate gum coating layer on the outer layer of the plant gum to maintain the suspension distribution state of the plant gum, wherein the parsley stem cell extract has the functions of resisting free radicals and reconstructing a skin dermoepidermal connecting layer, the tetrahydromethyl pyrimidine carboxylic acid can surround the components in the cells through a protein protective layer to protect the cells, the sodium hyaluronate with the molecular weight of thousands to millions contained in the 4D hyaluronic acid recovers the moisture retention capacity of the skin, and the carboxymethyl chitosan is used as a linear structure animal polysaccharide with positive charges to help to eliminate the free radicals generated by the cells, then the aging process of cells is relieved, the hydrolyzed algae extract can reduce the sensitivity of skin and enhance the use experience of the repair essence, wherein the alkalescence of triethanolamine is used for reducing the damage of the acidic essence to the skin; according to the invention, the biological carbohydrate gum and the plant gum components arranged in the repair essence are utilized, so that the powdery particles of actinidine and apple polyphenol in the plant gum are suspended in the essence, the plant gum is spread on the skin in the massage process in the use of the repair essence, and after the skin epidermal cells are repaired by the essence, the actinidine and the apple polyphenol in the plant gum smoothly permeate into the interior of the epidermis, so that the absorption effect of the skin on the functional components is promoted, and the use effect of the repair essence containing the plant stem cell extract is improved.
Preferably, the actinidine is obtained by juicing, concentrating and drying selected pulp; selecting pericarp of immature fruit, placing in hot water of 80-92 deg.C, keeping the temperature for 2-3min, extracting polyphenol substances with ethanol as solvent, and controlling the concentration of apple polyphenol added into vegetable gum at 90-130 ppm; actinidine extracted from kiwi fruit pulp has high extraction conversion rate, apple polyphenol has high content in immature peel, most of bacteria are eliminated through a high-temperature hot water disinfection process, meanwhile, the destruction to polyphenol substances is avoided, the strong antioxidation effect of apple polyphenol is utilized, free radicals in skin cells can be captured only through low concentration, the damage to epidermal cells is reduced, and the efficacy of plant gum in the repair essence containing the plant stem cell extract is improved.
The preparation method of the repair essence containing the plant stem cell extract is suitable for the repair essence containing the plant stem cell extract, and comprises the following steps:
s1, stem cell culture: selecting a celery stem cell, dividing the celery stem cell into a first part and a second part for culturing, adding the first part of the stem cell into a culture medium containing animal epidermal cells with normal metabolism, irradiating the first part of the stem cell with ultraviolet rays simulating sunlight, and adding the second part of the stem cell into the culture medium containing aged animal epidermal cells; the plant stem cells are cultured and screened under different conditions, so that the stem cells can generate active ingredients with corresponding environmental adaptability in the differentiation process, and the pertinence of the efficacy of the prepared essence is facilitated;
s2, sorting the extracts: after stem cells in S1 are cultured for 3-5 generations, offspring of the stem cells are divided into culture mediums with depleted nutrients and taken out, supernatant is separated out by centrifugation respectively, the supernatant is divided into AB two parts and is added into acidic methanol and 0.5% acetic acid solution respectively, and meanwhile, apple polyphenol and actinidine are added into the acidic methanol and 0.5% acetic acid solution, so as to obtain solution containing stem cell active substances; extracting active substance components required by the macaque and the apple from the supernatant respectively through different solvents, and further improving the content of corresponding active substances in the prepared essence;
s3, dissolving and concentrating: adding the active substance solution extracted in S2 into 1% inorganic salt solution, simultaneously cooling the inorganic salt solution at room temperature to 10-15 deg.C, filtering out the precipitated crystal, and oven drying at 36-39 deg.C; the dissolved active substances are separated out into crystals through inorganic salt solution, so that the adsorption of biological sugar gum in the subsequent process is facilitated;
s4, temperature-changing mixing: adding biogel into deionized water at 35-42 deg.C, blending to saturated dissolution state, adding crystal in S3, controlling solution temperature to decrease by 3-5 deg.C for 30-50S by constant temperature oscillator, and stopping when the temperature decreases to below room temperature to obtain vegetable gum; separating out biological sugar gum contained in the solution by a step-type cooling process to wrap the biological sugar gum on particles of a crystal substance, and enabling the crystal particles to be in a suspended colloid phase state in the aqueous solution by utilizing an oscillator;
s5, essence blending: preparing a glycerol aqueous solution with the concentration of 20-25%, and then adding 4D hyaluronic acid, lecithin and a hydrolyzed algae extract with corresponding components; uniformly stirring the solution in one direction at 5-12 ℃, adding triethanolamine to adjust the pH value of the solution to be neutral, heating the solution to 32-38 ℃, adding biogel for oscillation, and simultaneously adding the vegetable gum in S4 until the liquid phase is uniform to obtain the repair essence; the temperature in the process of adding the biological carbohydrate gum is increased, so that the activity level of the biological carbohydrate gum in the aqueous solution is improved, the biological carbohydrate gum is favorably and uniformly distributed in all places in the solution, and the distribution balance of the vegetable gum is promoted.
Preferably, in the essence blending process, 5-7 parts of nut seed oil and 1-2 parts of buckwheat seed oil are also added in the process of adding the biogum sugar; wherein the temperature of the nut seed oil and buckwheat seed oil is controlled at 3-7 deg.C before adding; the oil added into the biogum can help to improve the oxidation resistance of the essence, and the oil needs to be slowly dispersed in the solution in the mixing process, so that the adding time of the vegetable gum is influenced; the oil is controlled at a lower temperature before being added, so that the oil is quickly dissipated through the temperature rise process after being added into the aqueous solution at a higher temperature, the addition condition of the vegetable gum can be met, the time consumption of the essence blending is reduced, and the preparation efficiency of the repair essence containing the plant stem cell extract is improved.
Preferably, the extraction steps of the biological carbohydrate gum are as follows:
I. soaking dried fucoidin in warm water at 25-42 deg.C for circular rinsing until the salt concentration detected in water is lower than 1, 6%, transferring the fucoidin to a basin, and mashing; salt content in the fucoidin is reduced through rinsing, and the fucoidin is fully unfolded in a warm water environment, so that the separation of the salt is promoted, and the extraction rate of the biogasose is prevented from being interfered;
II. Adding 75% ethylene glycol solution into the mashed basin in the step I for leaching, simultaneously adding 3-6% diluted acetic acid, performing centrifugal separation to obtain an extracting solution, and performing freeze drying;
III, adding the freeze-dried extract in the step II into a stirring tank, adding cellulase and pectinase, operating the stirring tank for 7-12h, drying the mixture to 25-40% of water content, and sieving through a 100-mesh sieve to obtain biological sugar gum; the reaction rate of cellulase and pectinase on the cell wall of the fucoidin is promoted by the operating stirring tank, and the freshness of the obtained biological carbohydrate gum is maintained.
Preferably, the oscillation frequency in the essence blending process is 0.3-0.5Hz, and the oscillation direction is opposite to the stirring direction; the added plant gum is suspended and distributed in the essence with the viscosity formed by the biological carbohydrate gum, so that the even distribution effect of the plant gum in the essence can be interfered by the overlarge centrifugal acting force and the reciprocating tangential force in the mixing process, and the balanced distribution state of the plant gum in the essence is met by adopting low-frequency directional oscillation, so that the application effect of the preparation method of the repair essence containing the plant stem cell extract is improved.
The invention has the following beneficial effects:
1. according to the invention, through the biological carbohydrate gum and the plant gum components arranged in the repair essence, the powdery particles of actinidine and apple polyphenol in the plant gum are suspended in the essence, and after the skin epidermal cells are repaired by the essence, the actinidine and apple polyphenol in the plant gum smoothly permeate into the interior of the epidermis, so that the absorption effect of the skin on the functional components is promoted.
2. The invention avoids the destruction to polyphenols in the high-temperature hot water disinfection process, and reduces the damage to epidermal cells by utilizing the strong antioxidation of apple polyphenol; the oil is controlled at a lower temperature before being added, so that the oil is quickly dissipated in the temperature rise process, and the time consumption of blending the essence is reduced; the low-frequency directional oscillation is adopted to meet the balanced distribution state of the vegetable gum in the essence.
Drawings
The invention is further described with reference to the following figures and embodiments.
FIG. 1 is a flow chart of a method for preparing a repair serum containing a plant stem cell extract according to the present invention;
FIG. 2 is a flow chart of the steps of extraction of bioglycan in the present invention;
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
As shown in fig. 1 to 2, the repair essence containing plant stem cell extract of the present invention comprises the following components in parts by weight: 70-80 parts of glycerol aqueous solution, 24-31 parts of bioglycan, 14-19 parts of vegetable gum, 12-16 parts of fibronectin, 9-11 parts of tetrahydro-methyl pyrimidine carboxylic acid, 5-8 parts of 4D hyaluronic acid, 4-6 parts of carboxymethyl chitosan, 3-6 parts of lecithin, 2-3 parts of hydrolyzed algae extract and 0.4-0.8 part of triethanolamine; wherein the vegetable gum comprises a parsley stem cell extract, actinidine, apple polyphenol and bioglycan, and the specific gravity of the three is 2:3: 8; the actinidine and the apple polyphenol are powdery particles, and a solid wrapping layer formed by biogasose is arranged on the outer surface of the particles; the biological carbohydrate gum in the glycerol aqueous solution is in a saturated dissolved state, so that the vegetable gum and the biological carbohydrate gum form a suspension colloid in the essence; when the repair essence is used, functional components in the repair essence need to be ensured to enter basal layer cells through gaps of skin epidermis so as to achieve the repair effect on the functions of the skin cells, so that the basal layer cells are gradually converted into cells with a spinous layer, a granular layer and a cuticle in skin metabolism, and then are finally converted into cutin to be removed in the metabolic cycle of the skin, and as the metabolic state of the cutin cells on the surface layer of the skin is related to the direct visual effect of the skin, and in the process that the epidermal cells are finally converted into the cutin to be removed, the absorption effect of the cells on external active substances is gradually reduced, so that the functional active substances in the repair essence can exert the repair effect corresponding to the skin only when entering the basal layer cells in the epidermis; therefore, the invention supplies water needed by keratinocytes in the epidermis by arranging the 4D hyaluronic acid, lecithin and the hydrolyzed algae extract, the barrier function between the granular layer and acanthon stratum cells is recovered, the smoothness of intercellular spaces is recovered, the glycerol and the biological carbohydrate gum regulate the viscosity performance of the essence, and the glycerol and the biological carbohydrate gum are matched with the biological carbohydrate gum coating layer on the outer layer of the plant gum to maintain the suspension distribution state of the plant gum, wherein the parsley stem cell extract has the functions of resisting free radicals and reconstructing a skin dermoepidermal connecting layer, the tetrahydromethyl pyrimidine carboxylic acid can surround the components in the cells through a protein protective layer to protect the cells, the sodium hyaluronate with the molecular weight of thousands to millions contained in the 4D hyaluronic acid recovers the moisture retention capacity of the skin, and the carboxymethyl chitosan is used as a linear structure animal polysaccharide with positive charges to help to eliminate the free radicals generated by the cells, then the aging process of cells is relieved, the hydrolyzed algae extract can reduce the sensitivity of skin and enhance the use experience of the repair essence, wherein the alkalescence of triethanolamine is used for reducing the damage of the acidic essence to the skin; according to the invention, the biological carbohydrate gum and the plant gum components arranged in the repair essence are utilized, so that the powdery particles of actinidine and apple polyphenol in the plant gum are suspended in the essence, the plant gum is spread on the skin in the massage process in the use of the repair essence, and after the skin epidermal cells are repaired by the essence, the actinidine and the apple polyphenol in the plant gum smoothly permeate into the interior of the epidermis, so that the absorption effect of the skin on the functional components is promoted, and the use effect of the repair essence containing the plant stem cell extract is improved.
As an embodiment of the invention, the actinidine is obtained by juicing, concentrating and drying selected pulp; selecting pericarp of immature fruit, placing in hot water of 80-92 deg.C, keeping the temperature for 2-3min, extracting polyphenol substances with ethanol as solvent, and controlling the concentration of apple polyphenol added into vegetable gum at 90-130 ppm; actinidine extracted from kiwi fruit pulp has high extraction conversion rate, apple polyphenol has high content in immature peel, most of bacteria are eliminated through a high-temperature hot water disinfection process, meanwhile, the destruction to polyphenol substances is avoided, the strong antioxidation effect of apple polyphenol is utilized, free radicals in skin cells can be captured only through low concentration, the damage to epidermal cells is reduced, and the efficacy of plant gum in the repair essence containing the plant stem cell extract is improved.
The preparation method of the repair essence containing the plant stem cell extract is suitable for the repair essence containing the plant stem cell extract, and comprises the following steps:
s1, stem cell culture: selecting a celery stem cell, dividing the celery stem cell into a first part and a second part for culturing, adding the first part of the stem cell into a culture medium containing animal epidermal cells with normal metabolism, irradiating the first part of the stem cell with ultraviolet rays simulating sunlight, and adding the second part of the stem cell into the culture medium containing aged animal epidermal cells; the plant stem cells are cultured and screened under different conditions, so that the stem cells can generate active ingredients with corresponding environmental adaptability in the differentiation process, and the pertinence of the efficacy of the prepared essence is facilitated;
s2, sorting the extracts: after stem cells in S1 are cultured for 3-5 generations, offspring of the stem cells are divided into culture mediums with depleted nutrients and taken out, supernatant is separated out by centrifugation respectively, the supernatant is divided into AB two parts and is added into acidic methanol and 0.5% acetic acid solution respectively, and meanwhile, apple polyphenol and actinidine are added into the acidic methanol and 0.5% acetic acid solution, so as to obtain solution containing stem cell active substances; extracting active substance components required by the macaque and the apple from the supernatant respectively through different solvents, and further improving the content of corresponding active substances in the prepared essence;
s3, dissolving and concentrating: adding the active substance solution extracted in S2 into 1% inorganic salt solution, simultaneously cooling the inorganic salt solution at room temperature to 10-15 deg.C, filtering out the precipitated crystal, and oven drying at 36-39 deg.C; the dissolved active substances are separated out into crystals through inorganic salt solution, so that the adsorption of biological sugar gum in the subsequent process is facilitated;
s4, temperature-changing mixing: adding biogel into deionized water at 35-42 deg.C, blending to saturated dissolution state, adding crystal in S3, controlling solution temperature to decrease by 3-5 deg.C for 30-50S by constant temperature oscillator, and stopping when the temperature decreases to below room temperature to obtain vegetable gum; separating out biological sugar gum contained in the solution by a step-type cooling process to wrap the biological sugar gum on particles of a crystal substance, and enabling the crystal particles to be in a suspended colloid phase state in the aqueous solution by utilizing an oscillator;
s5, essence blending: preparing a glycerol aqueous solution with the concentration of 20-25%, and then adding 4D hyaluronic acid, lecithin and a hydrolyzed algae extract with corresponding components; uniformly stirring the solution in one direction at 5-12 ℃, adding triethanolamine to adjust the pH value of the solution to be neutral, heating the solution to 32-38 ℃, adding biogel for oscillation, and simultaneously adding the vegetable gum in S4 until the liquid phase is uniform to obtain the repair essence; the temperature in the process of adding the biological carbohydrate gum is increased, so that the activity level of the biological carbohydrate gum in the aqueous solution is improved, the biological carbohydrate gum is favorably and uniformly distributed in all places in the solution, and the distribution balance of the vegetable gum is promoted.
In one embodiment of the invention, in the essence blending step, 5-7 parts of nut seed oil and 1-2 parts of buckwheat seed oil are added in the process of adding the biogum sugar; wherein the temperature of the nut seed oil and buckwheat seed oil is controlled at 3-7 deg.C before adding; the oil added into the biogum can help to improve the oxidation resistance of the essence, and the oil needs to be slowly dispersed in the solution in the mixing process, so that the adding time of the vegetable gum is influenced; the oil is controlled at a lower temperature before being added, so that the oil is quickly dissipated through the temperature rise process after being added into the aqueous solution at a higher temperature, the addition condition of the vegetable gum can be met, the time consumption of the essence blending is reduced, and the preparation efficiency of the repair essence containing the plant stem cell extract is improved.
As an embodiment of the present invention, the extraction step of the biological carbohydrate gum is as follows:
I. soaking dried fucoidin in warm water at 25-42 deg.C for circular rinsing until the salt concentration detected in water is lower than 1, 6%, transferring the fucoidin to a basin, and mashing; salt content in the fucoidin is reduced through rinsing, and the fucoidin is fully unfolded in a warm water environment, so that the separation of the salt is promoted, and the extraction rate of the biogasose is prevented from being interfered;
II. Adding 75% ethylene glycol solution into the mashed basin in the step I for leaching, simultaneously adding 3-6% diluted acetic acid, performing centrifugal separation to obtain an extracting solution, and performing freeze drying;
III, adding the freeze-dried extract in the step II into a stirring tank, adding cellulase and pectinase, operating the stirring tank for 7-12h, drying the mixture to 25-40% of water content, and sieving through a 100-mesh sieve to obtain biological sugar gum; the reaction rate of cellulase and pectinase on the cell wall of the fucoidin is promoted by the operating stirring tank, and the freshness of the obtained biological carbohydrate gum is maintained.
In one embodiment of the present invention, the oscillation frequency in the essence blending process is 0.3 to 0.5Hz, and the oscillation direction is opposite to the stirring direction; the added plant gum is suspended and distributed in the essence with the viscosity formed by the biological carbohydrate gum, so that the even distribution effect of the plant gum in the essence can be interfered by the overlarge centrifugal acting force and the reciprocating tangential force in the mixing process, and the balanced distribution state of the plant gum in the essence is met by adopting low-frequency directional oscillation, so that the application effect of the preparation method of the repair essence containing the plant stem cell extract is improved.
When in use, functional components in the skin are required to enter basal layer cells through gaps of the skin epidermis so as to achieve the effect of repairing the functions of the skin cells, so that the basal layer cells are gradually converted into cells with a spinous layer, a granular layer and a cuticle layer in the skin metabolism, and then are finally converted into cutin to be removed in the skin metabolism period; the 4D hyaluronic acid, lecithin and the hydrolyzed algae extract are arranged to supplement water needed by keratinocytes in the skin, barrier function between a granular layer and cells with acanthosis is achieved, smoothness of cell gaps is recovered, the glycerol and the biological carbohydrate gum adjust viscosity performance of the essence, the essence is matched with a biological carbohydrate gum wrapping layer on the outer layer of the vegetable gum, and the suspension distribution state of the vegetable gum is maintained, wherein weak alkalinity of triethanolamine is used for reducing damage of the acidic essence to the skin; the actinidine extracted from the kiwi fruit pulp has high extraction conversion rate, the apple polyphenol has high content in immature peel, most bacteria in the kiwi fruit pulp are eliminated through a high-temperature hot water disinfection process, meanwhile, the destruction to polyphenol substances is avoided, and by utilizing the strong antioxidation effect of the apple polyphenol, free radicals in skin cells can be captured only at a low concentration, so that the damage to epidermal cells is reduced; the oil is controlled at a lower temperature before being added, so that the oil can be quickly dissipated through a heating process after being added into a higher-temperature aqueous solution, the adding condition of the vegetable gum can be met, and the time consumption of the blending of the essence is reduced; the low-frequency directional oscillation is adopted to meet the balanced distribution state of the vegetable gum in the essence.
In order to verify the practical use effect of the repair essence containing the plant stem cell extract according to the present invention, the following experiment was performed.
Starting a new product activity in a certain beauty parlor, providing the repair essence liquid for the women who care skin for trial, randomly selecting 30 women who use the brand A facial skin repair essence liquid, dividing the women into three groups by 10 people, respectively using the repair essence liquid, continuing to use the brand A repair essence liquid and replacing the repair essence liquid with the brand B repair essence liquid, and recording the facial skin conditions of five days after using the repair essence liquid in the period from seventies and ten days to eighty days;
experimental group 1: the repair essence of the invention is used
Experimental group 2: continuously using first brand repair essence
Experimental group 3: replacing and using brand B repairing essence
The results recorded by the experimental groups show that the facial skin condition can be improved by using the repair essence, the brand A repair essence and the brand B repair essence in the market, the repair essence in the invention has a better repair speed, and the repair essence has a better and obvious improvement effect on the facial skin after being used for one month.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. The repair essence containing the plant stem cell extract is characterized by comprising the following components in parts by weight: 70-80 parts of glycerol aqueous solution, 24-31 parts of bioglycan, 14-19 parts of vegetable gum, 12-16 parts of fibronectin, 9-11 parts of tetrahydro-methyl pyrimidine carboxylic acid, 5-8 parts of 4D hyaluronic acid, 4-6 parts of carboxymethyl chitosan, 3-6 parts of lecithin, 2-3 parts of hydrolyzed algae extract and 0.4-0.8 part of triethanolamine; wherein the vegetable gum comprises actinidine, apple polyphenol and biological sugar gum, and the specific gravity of the three is 2:3: 8; the actinidine and the apple polyphenol are powdery particles, and a solid wrapping layer formed by biogasose is arranged on the outer surface of the particles; the biological carbohydrate gum in the glycerol aqueous solution is in a saturated dissolved state, so that the vegetable gum and the biological carbohydrate gum form a suspension colloid in the essence.
2. The repair essence containing plant stem cell extract according to claim 1, wherein: the actinidine is obtained by juicing, concentrating and drying selected pulp; the apple polyphenol is prepared by selecting pericarp of immature fruit, placing in hot water of 80-92 deg.C, keeping the temperature for 2-3min, extracting polyphenol substances with ethanol as solvent, and controlling the concentration of apple polyphenol added into vegetable gum at 90-130 ppm.
3. A method for preparing a repair essence containing a plant stem cell extract, which is applied to the repair essence containing a plant stem cell extract according to claims 1-2, and comprises the following steps:
s1, stem cell culture: selecting a celery stem cell, dividing the celery stem cell into a first part and a second part for culturing, adding the first part of the stem cell into a culture medium containing animal epidermal cells with normal metabolism, irradiating the first part of the stem cell with ultraviolet rays simulating sunlight, and adding the second part of the stem cell into the culture medium containing aged animal epidermal cells;
s2, sorting the extracts: after stem cells in S1 are cultured for 3-5 generations, offspring of the stem cells are divided into culture mediums with depleted nutrients and taken out, supernatant is separated out by centrifugation respectively, the supernatant is divided into AB two parts and is added into acidic methanol and 0.5% acetic acid solution respectively, and meanwhile, apple polyphenol and actinidine are added into the acidic methanol and 0.5% acetic acid solution, so as to obtain solution containing stem cell active substances;
s3, dissolving and concentrating: adding the active substance solution extracted in S2 into 1% inorganic salt solution, simultaneously cooling the inorganic salt solution at room temperature to 10-15 deg.C, filtering out the precipitated crystal, and oven drying at 36-39 deg.C;
s4, temperature-changing mixing: adding biogel into deionized water at 35-42 deg.C, blending to saturated dissolution state, adding crystal in S3, controlling solution temperature to decrease by 3-5 deg.C for 30-50S by constant temperature oscillator, and stopping when the temperature decreases to below room temperature to obtain vegetable gum;
s5, essence blending: preparing a glycerol aqueous solution with the concentration of 20-25%, and then adding 4D hyaluronic acid, lecithin and a hydrolyzed algae extract with corresponding components; uniformly stirring the raw materials in one direction at 5-12 ℃, adding triethanolamine to adjust the pH value to be neutral, heating the solution to 32-38 ℃, adding biogel for oscillation, and simultaneously adding the vegetable gum in S4 until the liquid phase is uniform to obtain the repair essence.
4. The method for preparing the repair essence containing plant stem cell extract according to claim 3, wherein the method comprises the following steps: in the essence blending procedure, 5-7 parts of nut seed oil and 1-2 parts of buckwheat seed oil are also added in the process of adding the biogum sugar; wherein the nut seed oil and buckwheat seed oil are controlled at a temperature of 3-7 deg.C before addition.
5. The method for preparing the repair essence containing plant stem cell extract according to claim 4, wherein; the extraction steps of the biological carbohydrate gum are as follows:
I. soaking dried fucoidin in warm water at 25-42 deg.C for circular rinsing until the salt concentration detected in water is lower than 1, 6%, transferring the fucoidin to a basin, and mashing;
II. Adding 75% ethylene glycol solution into the mashed basin in the step I for leaching, simultaneously adding 3-6% diluted acetic acid, performing centrifugal separation to obtain an extracting solution, and performing freeze drying;
and III, adding the freeze-dried extract in the step II into a stirring tank, adding cellulase and pectinase, operating the stirring tank for 7-12h, drying the mixture until the water content is 25-40%, and sieving by using a 100-mesh sieve to obtain the biological carbohydrate gum.
6. The method for preparing the repair essence containing plant stem cell extract according to claim 3, wherein the method comprises the following steps: the oscillation frequency in the essence blending process is 0.3-0.5Hz, and the oscillation direction is opposite to the stirring direction.
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