CN112079674A - Preparation method of desert algae composite bacterial fertilizer - Google Patents
Preparation method of desert algae composite bacterial fertilizer Download PDFInfo
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- CN112079674A CN112079674A CN202011006162.8A CN202011006162A CN112079674A CN 112079674 A CN112079674 A CN 112079674A CN 202011006162 A CN202011006162 A CN 202011006162A CN 112079674 A CN112079674 A CN 112079674A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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Abstract
The invention provides a preparation method of desert algae composite bacterial fertilizer, which comprises the following steps: 1) centrifuging the cultured desert algae cell culture solution, removing supernatant, collecting algae cells, desalting, and performing ultrahigh-pressure homogenizing and crushing to obtain desert algae crushed solution; 2) adding the spore mother liquor into the desert algae crushing liquid to obtain a fungus-algae mixed liquor; 3) and crushing the inorganic fertilizer, mixing and dissolving the inorganic fertilizer according to a proportion, and adding the inorganic fertilizer into the bacteria-algae mixed solution to obtain the desert algae composite bacterial fertilizer. The desert algae broken liquid is used for preparing the composite bacterial fertilizer, the desert algae composite bacterial fertilizer is a composite microbial fertilizer which integrates microorganisms, organic nutrients and inorganic nutrients, the nutrients are comprehensive, the functions are complete, and higher economic benefits and ecological benefits can be generated.
Description
Technical Field
The invention relates to the technical field of agricultural biology, in particular to a preparation method of desert algae composite bacterial fertilizer.
Background
In recent years, the application of chemical fertilizers in large quantities has caused a very serious negative impact on the soil in agricultural production. The using amount of the fertilizer is increased year by year, but the effective utilization rate of the fertilizer and the yield increasing effect of the fertilizer are reduced, and excessive nitrogen, phosphorus and potassium can cause soil hardening, so that the fertilizer efficiency of the soil is weakened, and the original ecological balance state in the soil is broken. And relevant researches show that the algae fertilizer can improve soil fertility, increase crop yield, improve crop quality, improve soil physicochemical property and reduce environmental pollution, and is an ideal fertilizer for promoting ecological agriculture, organic agriculture and producing green products.
Desert algae is a specific ecological group of terrestrial algae, mainly referred to as algae organisms growing in desert areas. Desert algae are much more vital than common freshwater green algae or seaweeds, because the extremely harsh desert living environment makes desert algae cells have very specific components and functions: the method has the advantages that the desert environment is naturally lack of water, so that the CGF activity of desert algae cells is high, and the CGF content of the desert algae cells is higher than that of common green algae; ② the desert algae cell contains a large amount of unsaturated fatty acid; ③ the desert algae cell contains a large amount of extracellular polysaccharide (rich alkaline polysaccharide); fourthly, the desert algae has thick cell wall (cellulose component) and can effectively prevent excessive water loss. Chlorella from desert contains natural active substances and mineral elements beneficial to plant growth and development, including polysaccharide, phenolic polymeric compound, unsaturated fatty acid, plant growth regulating substance, and nitrogen, phosphorus, potassium, and microelements such as iron, molybdenum, boron, etc., and can improve soil nutrient status and promote plant growth.
Disclosure of Invention
The invention aims to provide a preparation method of a desert algae composite bacterial fertilizer, which is a composite microbial fertilizer integrating microorganisms, organic nutrients and inorganic nutrients, has comprehensive nutrients and complete functions, and can generate higher economic benefit and ecological benefit.
In order to achieve the purpose, the invention adopts the technical scheme that:
a preparation method of desert algae composite bacterial fertilizer comprises the following steps:
1) centrifuging the cultured desert algae cell culture solution, removing supernatant, collecting algae cells, desalting, and performing ultrahigh-pressure homogenizing and crushing to obtain desert algae crushed solution;
2) adding the spore mother liquor into the desert algae crushing liquid to obtain a fungus-algae mixed liquor;
3) and crushing the inorganic fertilizer, mixing and dissolving the inorganic fertilizer according to a proportion, and adding the inorganic fertilizer into the bacteria-algae mixed solution to obtain the desert algae composite bacterial fertilizer.
Preferably, the inorganic fertilizer is ammonium chloride, monoammonium phosphate or potassium chloride.
Preferably, the method comprises the following specific steps:
1) centrifuging the cultured desert algae cell culture solution with the cell concentration of 107 cells/ml for 8-10min at the temperature of 4 ℃ and at the rpm of 5000-10000, discarding the supernatant, collecting the algae cells, desalting, diluting the desalted algae cells with purified water, and crushing at low temperature and high pressure by using an ultrahigh pressure homogenizer to obtain a desert algae crushed solution;
2) adding 10-15% of spore mother liquor with the cell concentration of 20 hundred million/ml into desert algae broken liquid to obtain bacteria-algae mixed liquid;
3) and crushing ammonium chloride, monoammonium phosphate and potassium chloride, mixing and dissolving the crushed materials according to the weight ratio of 12-20% of ammonium chloride, 3-10% of monoammonium phosphate and 2-8% of potassium chloride, and adding the mixture into the bacteria-algae mixed solution to obtain the desert algae composite bacterial fertilizer.
The invention has the beneficial effects that:
(1) organic nitrogen, phosphorus and other nutrient components contained in the desert algae can be utilized to reduce the dosage of inorganic fertilizer in the fertilizer compatibility and reduce the fertilizer cost.
(2) The nitrogen-fixing, phosphate-solubilizing and potassium-solubilizing bacteria contained in the fertilizer can improve the fertilizer effect and improve the fertilizer utilization rate.
(3) The resource utilization efficiency of the desert algae is improved, and the economic benefit is improved.
Drawings
FIG. 1 is a process flow chart of the preparation method of desert algae composite bacterial manure.
Detailed Description
The technical solution of the present invention will be described in detail by specific examples.
Example 1
A preparation method of desert algae composite bacterial fertilizer comprises the following process steps:
1) the concentration of the cultured cells was 107Centrifuging desert algae cell culture solution per ml at 10000rpm and 4 deg.C for 10min, discarding supernatant, collecting algae cells and desalting, diluting the desalted algae cells with purified water, and crushing at low temperature and high pressure with ultrahigh pressure homogenizer to obtain desert algae crushed solution;
2) adding the spore mother liquor with the cell concentration of 20 hundred million/ml into the desert algae crushing liquid according to the amount of 10 percent to obtain a bacteria-algae mixed liquid;
3) and crushing the ammonium chloride, the monoammonium phosphate and the potassium chloride, mixing and dissolving the crushed materials according to the weight ratio of 12% of the ammonium chloride, 3% of the monoammonium phosphate and 2% of the potassium chloride, and adding the mixture into the bacteria-algae mixed solution to obtain the desert algae composite bacterial fertilizer.
Example 2
A preparation method of desert algae composite bacterial fertilizer comprises the following process steps:
1) the concentration of the cultured cells was 107Centrifuging desert algae cell culture solution per ml at 10000rpm and 4 deg.C for 10min, discarding supernatant, collecting algae cells and desalting, diluting the desalted algae cells with purified water, and crushing at low temperature and high pressure with ultrahigh pressure homogenizer to obtain desert algae crushed solution;
2) adding the spore mother liquor with the cell concentration of 20 hundred million/ml into the desert algae crushing liquid according to the amount of 15 percent to obtain bacteria-algae mixed liquid;
3) and crushing the ammonium chloride, the monoammonium phosphate and the potassium chloride, mixing and dissolving the crushed materials according to the weight ratio of 18% of the ammonium chloride, 7% of the monoammonium phosphate and 5% of the potassium chloride, and adding the mixture into the bacteria-algae mixed solution to obtain the desert algae composite bacterial fertilizer.
Example 3
A preparation method of desert algae composite bacterial fertilizer comprises the following process steps:
1) the concentration of the cultured cells was 107Centrifuging desert algae cell culture solution per ml at 5000rpm and 4 deg.C for 8min, discarding supernatant, collecting algae cells and desalting, diluting the desalted algae cells with purified water, and crushing at low temperature and high pressure with ultrahigh pressure homogenizer to obtain desert algae crushed solution;
2) adding the spore mother liquor with the cell concentration of 20 hundred million/ml into the desert algae crushing liquid according to the amount of 15 percent to obtain bacteria-algae mixed liquid;
3) and crushing the ammonium chloride, the monoammonium phosphate and the potassium chloride, mixing and dissolving the crushed materials according to the weight ratio of 20% of the ammonium chloride, 10% of the monoammonium phosphate and 8% of the potassium chloride, and adding the mixture into the bacteria-algae mixed solution to obtain the desert algae composite bacterial fertilizer.
The desert algae composite bacterial fertilizer is a composite microbial fertilizer integrating microorganisms, organic nutrients and inorganic nutrients, and comprises natural bioactive substances and mineral nutrient elements which are beneficial to plant growth and development, such as protein, lipid, polysaccharide, dietary fiber, vitamins, trace elements, minerals, folic acid, chlorophyll and the like besides soil probiotics. The desert algae composite bacterial fertilizer has the characteristics of improving soil fertility, improving soil micro-ecological environment, increasing crop yield, improving crop quality and the like, and is suitable for green and organic agricultural planting.
Although the present invention has been described in detail with reference to the specific embodiments, the present invention is not limited to the above embodiments, and various changes and modifications without inventive changes may be made within the knowledge of those skilled in the art without departing from the spirit of the present invention.
Claims (3)
1. The preparation method of the desert algae composite bacterial fertilizer is characterized by comprising the following steps:
1) centrifuging the cultured desert algae cell culture solution, removing supernatant, collecting algae cells, desalting, and performing ultrahigh-pressure homogenizing and crushing to obtain desert algae crushed solution;
2) adding the spore mother liquor into the desert algae crushing liquid to obtain a fungus-algae mixed liquor;
3) and crushing the inorganic fertilizer, mixing and dissolving the inorganic fertilizer according to a proportion, and adding the inorganic fertilizer into the bacteria-algae mixed solution to obtain the desert algae composite bacterial fertilizer.
2. The method for preparing desert algae composite bacterial fertilizer as claimed in claim 1, wherein the inorganic fertilizer is ammonium chloride, monoammonium phosphate and potassium chloride.
3. The preparation method of the desert algae composite bacterial fertilizer as claimed in claim 2, which is characterized by comprising the following specific steps:
1) centrifuging the cultured desert algae cell culture solution with the cell concentration of 107 cells/ml for 8-10min at the temperature of 4 ℃ and at the rpm of 5000-10000, discarding the supernatant, collecting the algae cells, desalting, diluting the desalted algae cells with purified water, and crushing at low temperature and high pressure by using an ultrahigh pressure homogenizer to obtain a desert algae crushed solution;
2) adding 10-15% of spore mother liquor with the cell concentration of 20 hundred million/ml into desert algae broken liquid to obtain bacteria-algae mixed liquid;
3) and crushing ammonium chloride, monoammonium phosphate and potassium chloride, mixing and dissolving the crushed materials according to the weight ratio of 12-20% of ammonium chloride, 3-10% of monoammonium phosphate and 2-8% of potassium chloride, and adding the mixture into the bacteria-algae mixed solution to obtain the desert algae composite bacterial fertilizer.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113336608A (en) * | 2021-07-14 | 2021-09-03 | 新疆金正生物科技有限公司 | Humic acid-containing water-soluble fertilizer for desert algae |
| CN113354489A (en) * | 2021-07-14 | 2021-09-07 | 新疆金正生物科技有限公司 | Desert algae macroelement water-soluble fertilizer |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103641640A (en) * | 2013-12-15 | 2014-03-19 | 岳万华 | Activity-enhanced algae microorganism fertilizer and preparation method thereof |
| CN106083436A (en) * | 2016-06-17 | 2016-11-09 | 肥西七色土生态农业开发有限公司 | A kind of liquid nutritional liquid of soilless culture Herba Allii Schoenoprasi and preparation method thereof |
| CN107973623A (en) * | 2017-11-22 | 2018-05-01 | 南昌大学 | Multi-form microalgae bio-fertilizer and preparation method thereof |
-
2020
- 2020-09-23 CN CN202011006162.8A patent/CN112079674A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103641640A (en) * | 2013-12-15 | 2014-03-19 | 岳万华 | Activity-enhanced algae microorganism fertilizer and preparation method thereof |
| CN106083436A (en) * | 2016-06-17 | 2016-11-09 | 肥西七色土生态农业开发有限公司 | A kind of liquid nutritional liquid of soilless culture Herba Allii Schoenoprasi and preparation method thereof |
| CN107973623A (en) * | 2017-11-22 | 2018-05-01 | 南昌大学 | Multi-form microalgae bio-fertilizer and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 新疆师范大学沙漠藻研究院: "来自沙漠的古老营养——沙漠藻肥", 《新疆师范大学沙漠藻研究院网站》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113336608A (en) * | 2021-07-14 | 2021-09-03 | 新疆金正生物科技有限公司 | Humic acid-containing water-soluble fertilizer for desert algae |
| CN113354489A (en) * | 2021-07-14 | 2021-09-07 | 新疆金正生物科技有限公司 | Desert algae macroelement water-soluble fertilizer |
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