CN112063516A - Microinjection system for testudinate eggs - Google Patents

Abstract

The invention belongs to the technical field of microinjection, and particularly relates to a microinjection system for testudinate eggs. The invention comprises a fixing device for fixing turtle eggs and soft-shelled turtle eggs and a microinjection device for injecting substances into the turtle eggs and soft-shelled turtle eggs, wherein the fixing device comprises a movable egg tray for placing the turtle eggs and soft-shelled turtle eggs; the microinjection apparatus includes a microinjector and a control assembly for controlling an injection amount and an injection depth of the microinjector. The injection device is provided with the fixing device and the microinjection device, so that the testudinate eggs can be fixed, the testudinate eggs are stable and immobile when being injected, injection substances can be accurately injected to a specified position conveniently, the injection substances can be quantitatively injected into the testudinate eggs, the depth of the microinjection needle entering the eggshell can be controlled, and the accuracy of an experiment is further improved.

Description

A microinjection system for turtle eggs

technical field

The invention belongs to the field of microinjection, in particular to a microinjection system for turtle eggs.

Background technique

Microinjection technology is a technology that uses microinjection needles to inject foreign genes or other substances into cells, and is one of the important technical means of modern bioengineering.

Turtles are also known as golden turtles, tortoises, mud turtles and mountain turtles; most turtles are carnivorous, feeding on worms, snails, shrimp and small fish, as well as the stems and leaves of plants. Turtle, also known as soft-shelled turtle, is rich in high-quality protein, amino acids, minerals, trace elements and vitamins A, B1, B2, etc. "The name. Turtles and soft-shelled turtles are one of the common turtles and are the oldest reptiles in existence.

In the existing experimental research on turtle eggs (referring to the collective name of turtle eggs and turtle eggs), it is usually necessary to inject dyes, viruses, pharmaceutical reagents and plasmids into turtle eggs before proceeding to the next step. However, in the existing operation, it is impossible to quantitatively inject substances into the turtle eggs, and it is impossible to control the depth of the microinjection needle injected into the turtle eggs, so that the substances cannot be accurately injected into the specific positions of the turtle eggs. In some laboratories, there is no specific device for fixing turtle eggs, which makes it difficult to fix turtle eggs.

SUMMARY OF THE INVENTION

In order to overcome the above-mentioned defects in the prior art, the present invention provides a microinjection system for turtle eggs. The invention can not only control the amount of substances injected into the turtle eggs and the injection depth of the microinjection needle, but also has the advantage of stabilizing the turtle eggs.

To achieve the above object, the present invention adopts the following technical solutions:

A microinjection system for turtle eggs, the system includes a fixing device for fixing turtle eggs/turtle eggs and a microinjection device for injecting substances into turtle eggs/turtle eggs, the fixing device includes for placing turtle eggs/ The movable egg tray of turtle eggs; the microinjection device includes a microinjector and a control component for controlling the injection volume and injection depth of the microinjector.

Preferably, the egg holder is provided with a holder cavity for fixing tortoise/turtle eggs and a moving component for moving the holder cavity.

Preferably, the microinjector includes a microinjection barrel loaded with the injected substance and a microinjection needle for extending into the eggshell.

Preferably, the control assembly includes a first telescopic unit for controlling the depth of the microinjector extending into/out of the egg shell and a second telescopic unit for controlling the injection volume of the microinjector.

Preferably, the holder cavity includes an oval shape and a circular shape.

Preferably, the moving assembly includes a third telescopic unit and a fourth telescopic unit that make the support cavity move in a horizontal direction and a vertical direction, respectively.

Preferably, the microinjection barrel includes a barrel body and a push-pull member inserted in the barrel body for propelling the injected substance into the egg shell.

Preferably, the microinjection device further comprises a first fixing member for fixing the barrel body and a second fixing member fixedly connected with the push-pull member.

Preferably, the microinjection device further includes a base mounted with a second telescopic unit, and the control assembly further includes a controller mounted on the base, the controller is electrically connected to the first telescopic unit and the second telescopic unit, respectively.

Preferably, the egg tray is further provided with a chute for restricting the movement of the tray cavity along the horizontal direction.

The advantages of the present invention are:

(1) The present invention is provided with a fixing device and a microinjection device, not only can the turtle eggs be fixed, so that the turtle eggs are stable when the turtle eggs are injected, and it is convenient to accurately inject the injection material into the designated area. The position of the injection material can be quantitatively injected into the turtle eggs, and the depth of the microinjection needle into the eggshell can also be controlled to further improve the accuracy of the experiment.

(2) The first fixing member and the second fixing member provided in the present invention can fix the barrel body of the microinjection cylinder, which is convenient to improve the stability of the microinjector during operation.

(3) The moving component provided in the present invention is convenient for automatically injecting the next turtle egg/turtle egg after the injection of one of the turtle eggs/turtle eggs, which reduces the operation of manually moving the egg tray and the moving position is more accurate , improve the injection efficiency.

Description of drawings

Figure 1 is a schematic structural diagram of the present invention.

FIG. 2 is a schematic structural diagram of the fixing device of the present invention.

FIG. 3 is a schematic structural diagram of the microinjection device of the present invention.

FIG. 4 is a schematic view of the side structure of the fixing device of the present invention.

FIG. 5 is a schematic top view of the structure of the support cavity of the present invention.

FIG. 6 is a schematic structural diagram of the second fixing member of the present invention.

The meanings of the symbols in the figure are as follows:

1-Fixing device, 11-Egg tray, 111-Torting cavity, 112-Third telescopic unit, 113-Fourth telescopic unit, 114-Fixed seat, 115-Chute, 2-Microinjection device, 21-Microscope Syringe, 211-microinjection barrel, 2111-push-pull piece, 212-microinjection needle, 22-control assembly, 221-first telescopic unit, 222-second telescopic unit, 223-controller, 23-first fixation 231-block, 24-second fixing piece, 241-card slot, 25-base, 26-limiting piece.

Detailed ways

As shown in Figures 1-6, a microinjection system for turtle eggs, the system includes a fixing device 1 for fixing turtle eggs/turtle eggs and a microinjection device 2 for injecting substances into turtle eggs/turtle eggs .

As shown in FIG. 2 , the fixing device 1 includes a movable egg holder 11 for placing turtle eggs/turtle eggs; the egg holder 11 is provided with a holder cavity 111 for fixing the turtle eggs/turtle eggs and allowing the holder cavity 111 to move mobile components. The moving assembly includes a third telescopic unit 112 and a fourth telescopic unit 113 that make the support cavity 111 move in the horizontal direction and the vertical direction respectively. The third telescopic unit 112 and the fourth telescopic unit 113 are both electric telescopic rods or ball screws The structure, etc., can be moved in the horizontal direction or the vertical direction. Specifically, the non-telescopic end of the third telescopic unit 112 is fixed on the fixed seat 114 , and the telescopic end of the third telescopic unit 112 is connected to the bottom of the bracket cavity 111 . The telescopic end of the fourth telescopic unit 113 is fixed to the fixed seat 114, so that the fixed seat 114 can move vertically; the fixed seat 114 is also provided with a chute 115 for guiding the support cavity 111 to move in the horizontal direction, connecting The blocks can slide within the chute 115 .

As shown in Figure 4-5, since turtle eggs are oval and turtle eggs are round, the support cavity 111 includes an oval support cavity 111 and a circular support cavity 111, which is convenient for fixing and experimenting with different types. The support cavity 111 is provided with a sponge pad, which can increase the frictional force between the support cavity 111 and the egg shell, thereby facilitating the fixing of the egg shell.

As shown in FIG. 3 , the microinjection device 2 includes a microinjector 21 and a control component 22 for controlling the injection volume and injection depth of the microinjector 21 . The microinjector 21 includes a microinjection barrel 211 loaded with the injected substance and a microinjection needle 212 for protruding into the egg shell. The control assembly 22 includes a first telescopic unit 221 for controlling the depth of the microinjector 21 extending into/out of the egg shell and a second telescopic unit 222 for controlling the injection volume of the microinjector 21, and the movement direction of the first telescopic unit 221 It is parallel to the length direction of the cylinder body of the injection cylinder 211. Specifically, the first telescopic unit 221 moves in the vertical direction and matches with the horizontally placed support cavity 111, which is convenient for exerting force on the turtle eggs without causing the turtle eggs. The movement of the egg enables the microinjection needle 212 to quickly penetrate into the turtle egg. The microinjection barrel 211 includes a barrel body and a push-pull member 2111 connected with the barrel body for propelling the injected substance into the eggshell. The barrel body is provided with a microfluidic channel for accommodating the injected substance. The microinjection device 2 further includes a first fixing member 23 for fixing the barrel and a second fixing member 24 for fixing the push-pull member 2111 . The microinjection device 2 further includes a base 25 mounted with a second telescopic unit 222 , the first fixing member 23 and the second fixing member 24 are both installed on the base 25 , and the control assembly 22 further includes a controller 223 installed on the base 25 , the controller 223 is electrically connected to the first telescopic unit 221 and the second telescopic unit 222 respectively, and is used to precisely control the working conditions of the first telescopic unit 221 and the second telescopic unit 222 .

The base 25 is also provided with a limiting member 26 penetrating the second fixing member 24 , the limiting member 26 is parallel to the telescopic direction of the second telescopic unit 222 , and the limiting member 26 can limit the moving direction of the second fixing member 24 , and further It is convenient to improve the stability of the movement of the second fixing member 24 .

As shown in FIG. 6 , the second fixing member 24 is provided with a slot 241 for engaging the end of the push-pull member 2111 away from the cylinder body. The first fixing member 23 is provided with a groove for accommodating the cylinder body and a clamping block 231 for further fixing the cylinder body.

The working process of the device will be described in detail below with reference to the accompanying drawings in the embodiments.

First place the turtle/turtle egg to be studied in the corresponding support cavity 111, then adjust the position of the turtle/turtle egg under the microinjection needle 212, and set the first telescopic unit 221 on the controller 223 and the telescopic length of the second telescopic unit 222, the height of the drop of the microinjection needle 212 and the amount of advancement of the push-pull member 2111 can be determined, thereby controlling the depth of the microinjection needle 212 into the turtle/turtle egg and the entry of the injected substance The amount into the egg shell, and then control the third telescopic unit 112 to make the support cavity 111 move in the horizontal direction, so as to facilitate the microinjection of the next turtle/turtle egg.

The above are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention shall be included in the present invention. within the scope of protection.

Claims (10)

1. a microinjection system for turtle eggs, is characterized in that: the system comprises a fixing device (1) for fixing turtle eggs/turtle eggs and a microinjection device (1) for injecting substances into turtle eggs/turtle eggs 2), the fixing device (1) includes a movable egg tray (11) for placing turtle eggs/turtle eggs; the microinjection device (2) includes a microinjector (21) and is used for controlling A control assembly (22) for the injection volume of the microinjector (21) and the injection depth. 2. The microinjection system for turtle eggs according to claim 1, characterized in that: the egg holder (11) is provided with a holder cavity (111) for fixing tortoise eggs/turtle eggs and a holder for making the holder A mobile assembly for the movement of the cavity (111). 3. The microinjection system of a turtle egg according to claim 1, wherein the microinjector (21) comprises a microinjection barrel (211) loaded with injection substances and a microinjection barrel (211) for extending into the egg Microinjection needle (212) inside the shell. 4. The microinjection system for turtle eggs according to claim 3, wherein the control assembly (22) comprises a depth for controlling the microinjector (21) to extend into/out of the eggshell a first telescopic unit (221) and a second telescopic unit (222) for controlling the injection volume of the microinjector (21). 5 . The microinjection system for turtle eggs according to claim 2 , wherein the holding cavity ( 111 ) comprises an ellipse and a circle. 6 . 6 . The microinjection system for turtle eggs according to claim 2 , wherein the moving component comprises a third telescopic unit that makes the support cavity (111) move in the horizontal direction and in the vertical direction, respectively. 7 . (112) and a fourth telescopic unit (113). 7. The microinjection system of a turtle egg according to claim 4, wherein the microinjection barrel (211) comprises a barrel body and a microinjection device inserted in the barrel body for pushing into the eggshell Push-pull (2111) for injecting substances. 8. The microinjection system for turtle eggs according to claim 7, wherein the microinjection device (2) further comprises a first fixing member (23) for fixing the cylinder body and a The push-pull member (2111) is fixedly connected to the second fixing member (24). 9. The microinjection system for turtle eggs according to claim 8, wherein the microinjection device (2) further comprises a base (25) on which a second telescopic unit (222) is installed, The control assembly (22) further includes a controller (223) mounted on the base (25), and the controller (223) is electrically connected to the first telescopic unit (221) and the second telescopic unit (222) respectively. 10. The microinjection system for turtle eggs according to claim 2, wherein the egg holder (11) is further provided with a chute (115) that restricts the movement of the holder cavity (111) along the horizontal direction ).

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