CN101637411A - Tiny vein injection drug delivery system on body surface of experimental animal - Google Patents

Abstract

The present invention relates to a drug delivery device for experimental animals, especially a drug delivery device for small intravenous injection on the surface of experimental animals. The needle 2 is composed of the syringe 1, the experimental animal fixing compartment 5 fixes the experimental animal, the blood vessel clip 7 is clamped above the injection blood vessel, the syringe 1 is connected with the ultrafine injection needle 2, and the simple desktop magnifying glass is placed above the intravenous injection operation, and the injection can be performed The components of the device can also be used alone or in any combination according to actual operation needs. The beneficial effect of the invention is to make the process of micro-intravenous injection of experimental animals more accurate, efficient, convenient and fast.

Description

Experimental animal body surface micro intravenous injection drug delivery device

Technical field

The patent of the present invention relates to a drug delivery device for experimental animals, in particular to a drug delivery device for micro-vein injection on the body surface of experimental animals.

Background technique

Microvenous administration on the body surface is a commonly used method of drug administration in experimental animals, but due to its high technical difficulty, the success rate in actual operation is very low, especially in the long-term administration process, often resulting in failure of the entire experiment due to the failure of the administration operation. Finish.

At present, the microvenous administration methods of experimental animals are mostly manually operated. The traditional operation process is to place the mice in an upside-down beaker, expose the tail of the mice to the outside of the beaker, and inject the mice into the tail vein with a 1 ml syringe. When using this method to operate, the experimental mice cannot be fixed well, and the experimental animals are always in a struggling state, which often leads to the inability to insert the needle or the needle punctures the blood vessel after the animal struggles, resulting in the failure of the experiment. In addition, when using this method for tail vein injection, because the tail vein blood vessels of mice are relatively small and difficult to fill, the needle insertion is inaccurate, or the resistance during injection after needle insertion is relatively high, resulting in local swelling and whitening, and the injection fails. rate is low.

Patent CN: 200810018231 Use a special rod clamp to fix the hand shape of the operator when injecting the tail vein of mice. The position and depth of the needle insertion are consistent with the hand shape for each subsequent injection, but due to individual differences in mice Large size, short tail, uneven thickness, using the same needle insertion depth and angle, cannot meet the actual needs of all experimental mice, so the effect of this patent is not ideal in actual operation, and its application is limited.

It is currently reported that the syringe needles used are relatively thick, and it is difficult to penetrate into the microvein of experimental animals, or even if it penetrates into the microvein, the needle is too thick to easily puncture the blood vessel wall and cause the liquid to leak, resulting in injection failure.

Contents of the invention

In order to overcome the deficiencies in the administration of microintravenous injection of the above-mentioned experimental animals. The patent of the present invention provides a drug delivery device for microvein injection on the surface of experimental animals. The device can simplify the operation process of microvenous injection on the surface of experimental animals, and can make the operation process more accurate, convenient and efficient.

In order to achieve the above object, the technical scheme of the patent of the present invention is as follows:

The experimental animal body surface micro-venous injection drug delivery device is composed of an experimental animal fixed chamber 5, a simple desktop magnifying glass, a blood vessel clip 7 and a syringe 1 with an ultra-fine injection needle 2, the experimental animal fixed chamber 5 fixes the experimental animal, and the blood vessel clip 7 Clipped above the injection vessel, the syringe 1 is connected to the ultrafine injection needle 2, and the simple desktop magnifying glass is placed above the intravenous injection operation, and the injection can be performed. The components of the device can also be used alone or in any combination according to actual operation needs.

1. Fixed chamber for experimental animals:

In order to overcome the problem that the experimental animals (rats, mice) cannot be fixed well during the operation, resulting in the inability to insert needles, or the experimental animals struggle after inserting the needles, resulting in injection failures, the experimental animal fixing chamber 5 of the present invention is equipped with a removable The cylindrical container of the extraction partition 4 and the fixed compartment cover 9 for the experimental animal. The fixed compartment 5 for the experimental animal has a slot for extracting the partition 4. According to the actual size of the experimental animal, select a different slot position to insert the extractable compartment. The partition 4 can fix the experimental animals in the smallest space, effectively reducing the struggling range of the experimental animals. In order to facilitate the rat tail vein injection operation, set the experimental animal tail piercing hole 6 in the center of the fixed bin cover 9 of the experimental animal, and pass the experimental animal tail through the hole. The position will not change with the twisting of animal limbs, and the operation is easy and smooth.

2. Simple desktop magnifying glass:

Because the small veins of experimental animals are small and thin, it is difficult for the operator to determine the position and depth of the small veins, and it is also difficult to determine whether the needle is in the blood vessel when injecting the small veins with the naked eye. The present invention has designed the simple desktop magnifying glass that magnification is more than 40 times, and simple desktop magnifying glass is made up of magnifying glass 3 and magnifying glass support 8, and magnifying glass support 8 supports magnifying glass 3 and is placed above the intravenous injection operation, and magnifying glass 3 can lift on the magnifying glass support 8, The height of the magnifying glass 3 can be adjusted according to the actual situation. Performing microvenous injection under the magnifying glass 3 can not only accurately locate the position of the microvenous, but also accurately determine whether the needle is in the microvenous.

3. Vascular clamp:

Traditional methods often use hot water bath and smearing ethanol to fill the small veins of experimental animals before injection, which is convenient for injection. However, the operation process of the hot water bath method is cumbersome, and the method of smearing ethanol makes the operation site slippery, making the operation more difficult. The present invention designs a crocodile-mouth-shaped blood vessel clip 7 that is convenient for clamping. During operation, the blood vessel clip 7 is used to clamp the upper part of the injection blood vessel, so that the tiny veins of experimental animals can be quickly filled and thickened, which is convenient for injection.

4. Syringes with ultra-fine injection needles:

In order to overcome the shortcoming that the syringe needle is thick, it is difficult to enter the microvein or easily puncture the blood vessel after entering the microvein, the present invention selects the syringe 1 with the ultrafine injection needle 2 having a diameter of 0.2 mm for microvenous injection, effectively avoiding the Operation failure caused by too thick injection needle.

The invention has the beneficial effects of making the process of micro-intravenous injection of experimental animals more accurate, efficient, convenient and fast.

Description of drawings

Fig. 1 is a structural schematic diagram of the present invention.

Fig. 2 is a schematic diagram of the structure of a syringe with an ultrafine needle.

Fig. 3 is a schematic diagram of the structure of a simple desktop magnifying glass.

Fig. 4 is a schematic diagram of the structure of the experimental animal fixed chamber.

In the figure: 1. Syringe, 2. Ultrafine injection needle, 3. Magnifying glass, 4. Extractable partition, 5. Fixed compartment for experimental animals, 6. Tail piercing hole for experimental animals, 7. Blood vessel clamp, 8. Magnifying glass Bracket, 9. The experimental animal is fixed to the cover.

Detailed ways

Example 1

Mouse tail vein injection administration:

As shown in Fig. 1 , the device for microvenous injection on the surface of experimental animals consists of a fixed chamber 5 for experimental animals, a simple desktop magnifying glass, a blood vessel clamp 7 and a syringe 1 with an ultrafine injection needle 2. The fixed chamber 5 for experimental animals is a There is a cylindrical container with an extractable partition 4 and a fixed compartment cover 9 for experimental animals. There is a slot for the extractable partition 4 on the fixed compartment 5 for experimental animals, and the tail of the experimental animal is set in the center of the fixed compartment cover 9 for experimental animals to pass through. Hole 6; the simple desktop magnifying glass is composed of a magnifying glass 3 and a magnifying glass bracket 8, the magnification of the magnifying glass 3 is more than 40 times, and the magnifying glass bracket 8 supports the magnifying glass 3 and is placed above the intravenous injection operation; The clip 7 clamps above the injection vessel; the syringe 1 has an ultrafine injection needle 2 with a diameter of 0.2 mm.

When performing tail vein injection of mice, first pick up the tail of the mouse, pass the tail of the mouse through the experimental animal tail hole 6 in the center of the experimental animal fixed compartment cover 9, and then place the mouse at the entrance of the experimental animal fixed compartment 5 , the mouse will instinctively crawl into the fixed compartment, and after the hind foot of the mouse enters the experimental animal fixed compartment 5, tighten the experimental animal fixed compartment cover 9, and select a suitable position for insertion according to the size of the mouse (juvenile mouse, adult mouse). The dividing plate 4 can be extracted to fix the experimental animal in the small space of the experimental animal fixed bin 5, so that the tail of the mouse can be exposed to the outside of the experimental animal fixed bin 5, and the rat tail can not be distorted along with the twisting of the mouse limbs. Mobile, easy to operate. After the mouse is fixed, the base of the tail of the mouse is clamped with a blood vessel clip 7 to fill the tail vein. The operator holds a syringe equipped with an ultra-fine needle in one hand and pinches the tail of the mouse with the other hand, and under the magnifying glass of a simple desktop magnifying glass 3 times Accurately locate the position and depth of the tail vein of the mouse, then use the syringe 1 with an ultra-fine injection needle 2 with a diameter of 0.2 mm to perform microvenous injection and then perform tail vein injection. See Table 1 for comparison of the effects of the tail vein injection of mice using the present invention and the traditional method.

Table 1 adopts traditional method and the present invention to carry out mouse tail vein injection effect comparison

Mice (only) success (only) Fail (only) Success rate Traditional method 20 6 14 30% this invention 20 19 1 95% ^#

It can be seen from Table 1 that the statistical results show that the present invention can significantly improve the success rate of mouse tail vein injection, and the difference compared with the traditional method is statistically significant, P<0.05.

Example 2

Rat tail vein injection administration:

The specific embodiment is the same as in Example 1, except that the experimental animal fixed compartment 5 and the experimental animal fixed compartment cover 9 of the mouse are replaced with the experimental animal fixed compartment 5 and the experimental animal fixed compartment cover 9 of the rat.

Example 3

Subcutaneous injection of mouse paw:

Expose the sole of the mouse’s foot, select the plantar vein of the foot under a magnifying glass, clamp the proximal end of the plantar vein of the mouse with a blood vessel clip 7, accurately locate the plantar vein of the mouse under the magnifying glass 3 of a simple desktop magnifying glass, and then use a A syringe 1 with an ultra-fine injection needle 2 having a diameter of 0.2 mm was injected into the plantar vein of the mouse.

Example 4

Sublingual intravenous injection in rats:

After the rats were anesthetized with ether, the rat’s mouth was opened to expose the sublingual vein, and the sublingual vein of the rat was accurately located under the magnifying glass 3 of a simple desktop magnifying glass. injection.

Claims (2)

1, tiny vein injection drug delivery system on body surface of experimental animal, it is characterized in that, this device is made of laboratory animal fixed bin (5), simple and easy desk-top magnifier, vascular clamp (7) and the syringe (1) that has ultra-fine injection needle (2), laboratory animal fixed bin (5) but for having the drum ladle that extracting dividing plate (4) and laboratory animal fixed bin cover (9), laboratory animal fixed bin (5) but on the slot of extracting dividing plate (4) is arranged, in laboratory animal fixed bin lid (9) central authorities the laboratory animal tail is set and passes hole (6); Simple and easy desk-top magnifier is made up of magnifier (3) and magnifying glass bracket (8), and the amplification of magnifier (3) is more than 40 times, and magnifier 3 is liftable on magnifying glass bracket 8, and magnifying glass bracket (8) supports magnifier (3) and places intravenous injection operation top; Vascular clamp (7) is the crocodile mouth shape, clamps injection blood vessel top with vascular clamp (7) during operation; It is 0.2 millimeter ultra-fine injection needle (2) that syringe (1) has diameter.

2, tiny vein injection drug delivery system on body surface of experimental animal according to claim 1, it is characterized in that, described laboratory animal fixed bin (5), simple and easy desk-top magnifier, vascular clamp (7) and have the syringe (1) of ultra-fine injection needle (2) can be separately or combination in any use.

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