CN111929885B - Parallel optical coherence tomography imaging equipment and auxiliary debugging method - Google Patents
Parallel optical coherence tomography imaging equipment and auxiliary debugging method Download PDFInfo
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Abstract
The parallel optical coherence tomography equipment comprises a cube spectroscope, wherein the input end of the cube spectroscope is connected with a lighting arm, and the output end of the cube spectroscope is connected with an imaging arm, a sample arm and a reference arm. The invention provides an auxiliary assembly and adjustment method for parallel optical coherence tomography equipment, which takes a point image or a minimum image of a light source as a reference, uses a collimating lens and an imaging lens to carry out non-reference iterative assembly and adjustment without references such as a collimator tube and the like, directly utilizes the position relation between the collimating lens and the light source and the position relation between the imaging lens and a camera image sensor, can realize interference imaging of a wide-spectrum extended light source through repeated iterative assembly and adjustment, has high assembly and adjustment precision, can greatly improve the assembly and adjustment efficiency of a system, calibrates a camera lens under the condition of no collimator tube, can collimate the light source under the condition of no calibration of the camera lens, and is accurate, reliable and simple and convenient to operate.
Description
Technical Field
The invention relates to the technical field of coherent tomography, in particular to a parallel optical coherent tomography device and an auxiliary debugging method.
Background
The Parallel Optical Coherence Tomography (POCT) technology is a further development of the traditional Optical Coherence Tomography (OCT), the POCT technology overcomes the defects that the traditional OCT depends on transverse scanning and has insufficient transverse resolution, the influence of the transverse scanning on a system is avoided in a multi-channel simultaneous Tomography mode, and the transverse resolution and the longitudinal resolution are improved to 1um by matching with a wide-spectrum light source, a large-numerical-aperture amplification objective lens and a high-quantum well depth camera. Has wide application prospect in the fields of ophthalmic examination, digestive tract endoscopic examination, biological tissue in vitro biopsy, medicine research and the like.
POCT optical systems are generally complex, sensitive to errors in the optical lens spacing, require high mounting accuracy, are difficult to adjust, and require multiple corrections and attempts even though conventional calibrated lenses and collimated light sources cannot be used directly. At present, the assembly and adjustment technical data of the POCT system is few, and a plurality of practical problems need to be solved.
Therefore, an auxiliary adjustment method for the parallel optical coherence tomography imaging device needs to be designed.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides the auxiliary debugging method of the parallel optical coherence tomography equipment, which has high debugging precision and simple and convenient operation and can greatly improve the debugging efficiency of a system.
The technical problem to be solved by the invention is realized by adopting the following technical scheme:
the parallel optical coherence tomography imaging device comprises a cubic spectroscope, wherein the input end of the cubic spectroscope is connected with an illumination arm, and the output end of the cubic spectroscope is connected with an imaging arm, a sample arm and a reference arm.
Preferably, the lighting arm comprises an auxiliary adjusting light source, the auxiliary adjusting light source is connected with a collimating lens, the collimating lens is arranged on the lighting arm linear motion platform, and the output end of the collimating lens is connected with the cubic spectroscope.
Preferably, the imaging arm comprises a CMOS camera, the CMOS camera corresponds to an imaging lens, the imaging lens is disposed on the imaging arm linear motion platform, and an output end of the imaging lens is connected to the cubic beam splitter.
Preferably, the sample arm comprises a reflector, a silicon chip is arranged on the reflector, the reflector is arranged on the sample arm linear motion platform, a sample microscope objective is arranged on the lens of the reflector correspondingly, and the sample microscope objective is connected with the cubic spectroscope.
Preferably, the reference arm comprises a reference arm linear motion platform, a reflector is arranged on the reference arm linear motion platform, a silicon wafer is arranged on the reflector, a reference microscope objective is correspondingly arranged on a lens of the reflector, and an output end of the reference microscope objective is connected with the cubic spectroscope.
Preferably, the auxiliary adjusting light source is an LD point light source, and the center wavelength of the auxiliary adjusting light source is 705nm.
Preferably, the surface of the reflector has a local burr scratch.
Preferably, the collimating lens is a single lens with positive focal power or a lens group consisting of two or more lenses.
Preferably, the image sensor of the CMOS camera is an area array detector, and is one of a CCD detector and a CMOS detector.
An auxiliary adjusting method of a parallel optical coherence tomography device comprises the following steps:
firstly, a collimating lens is a biconvex symmetrical convex lens with the focal length f1 of 50mm, the center of the lens is positioned at the center of a lens body, a vernier caliper is used for roughly positioning the collimating lens, and the distance d1 between the collimating lens and an auxiliary adjusting light source is equal to the focal length f1 of the collimating lens of 50mm, so that a roughly collimated light beam is obtained;
secondly, the imaging lens is an achromatic double cemented lens with a focal length f2 of 150mm, the center of the lens can be regarded as being at the center of the lens body, the distance between the center of the lens and a flange of the CMOS camera is d (2-1), the distance between the flange of the CMOS camera and the image sensor chip is d (2-2), the distance between the center of the lens and the image sensor chip is d2= d (2-1) + d (2-2), the imaging lens is roughly positioned by means of vernier calipers so that d2= f2=150mm, and after a light source passes through the collimating lens and the imaging lens, an image plane is basically aligned with the image sensor chip of the CMOS camera;
thirdly, connecting the CMOS camera with the display through a collecting card and a computer, and checking the point image of the light source through image processing software and the display after the CMOS camera images the light source;
fourthly, placing a light absorption baffle between the cubic spectroscope and the reference arm, and temporarily blocking the reference arm;
fifthly, driving the collimating lens to move by 5um step length by using the lighting arm linear motion platform, moving the collimating lens in any direction by taking the size reduction of the point image as a measurement reference, continuing to move if the size of the point image is reduced, otherwise moving in the opposite direction, determining the moving direction, gradually moving slightly, and entering the next step until the size of the point image is not reduced any more;
sixthly, driving the imaging lens to move by 5um step length by using the imaging arm linear motion platform, moving the imaging lens in any direction by taking the size reduction of the point image as a measurement reference, if the size of the point image is reduced, continuing to move, otherwise, moving in the opposite direction, determining the moving direction, then gradually moving slightly, and entering the next step until the size of the point image is not reduced;
seventhly, repeating the fifth step and the sixth step for multiple times until the size of the point image is not reduced, stopping installation and adjustment, and converting the auxiliary installation and adjustment light source into an LED light source;
and step eight, moving the reflector by using the sample arm linear motion platform with the step length of 1um until scratches or burrs on the reflector are clearly displayed on the display.
A ninth step of removing the light absorption baffle between the cubic spectroscope and the reference arm and placing the light absorption baffle between the cubic spectroscope and the sample arm;
step ten, moving the silicon chip on the reflector by using the sample arm linear motion platform in a step length of 1um until the image on the silicon chip is clearly displayed on the display;
and step eleven, removing the light absorption baffle, moving the silicon wafer on the reflector at a speed of 5 steps/second by using a reference arm linear motion platform at a step length of 0.2um, and generating an interference image to finish the whole assembly and adjustment process.
The invention has the advantages and positive effects that:
the invention provides an auxiliary assembly and adjustment method for parallel optical coherence tomography equipment, which takes a point image or a minimum image of a light source as a reference, uses a collimating lens and an imaging lens to carry out non-reference iterative assembly and adjustment without references such as a collimator tube and the like, directly utilizes the position relation of the collimating lens and the light source and the position relation of the imaging lens and a camera image sensor, can realize interference imaging of a wide-spectrum extended light source through repeated iterative assembly and adjustment, has high assembly and adjustment precision, can greatly improve the assembly and adjustment efficiency of a system, calibrates a camera lens under the condition of no collimator tube, and can collimate the light source under the condition of no calibrated lens camera, and is accurate, reliable and simple and convenient to operate.
Drawings
FIG. 1 is a schematic diagram of the setup configuration of the parallel optical coherence tomography apparatus of the present invention;
fig. 2 is a flow chart of the adjusting method of the parallel optical coherence tomography device of the invention.
In the figure:
2. an illumination arm; 2-1, auxiliary installation and adjustment of a light source; 2-2, a lighting arm linear motion platform; 2-3, a collimating lens;
3. an imaging arm; 3-1, CMOS camera; 3-2, imaging arm linear motion platform; 3-3, an imaging lens;
4. a sample arm; 4-1, a sample arm linear motion platform; 4-2, a reflector; 4-3, sample microscope objective;
5. a reference arm; 5-1, a reference arm linear motion platform; 5-2, a reflector; 5-3, a reference microscope objective;
6. a cube beam splitter.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When a component is referred to as being "connected" to another component, it can be directly connected to the other component or intervening components may also be present. When a component is referred to as being "disposed on" another component, it can be directly on the other component or intervening components may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
The embodiments of the invention will be described in further detail below with reference to the accompanying drawings:
as shown in fig. 1, the parallel optical coherence tomography apparatus of the present invention includes a cube beam splitter 6, an input end of the cube beam splitter 6 is connected to an illumination arm 2, and an output end of the cube beam splitter 6 is connected to an imaging arm 3, a sample arm 4 and a reference arm 5.
Further, the lighting arm 2 comprises an auxiliary adjusting light source 2-1, the auxiliary adjusting light source 2-1 is connected with a collimating lens 2-3, the collimating lens 2-3 is arranged on the lighting arm linear motion platform 2-2, and the output end of the collimating lens 2-3 is connected with the cubic spectroscope 6.
Further, the imaging arm 3 comprises a CMOS camera 3-1, the CMOS camera 3-1 is correspondingly provided with an imaging lens 3-3, the imaging lens 3-3 is arranged on the imaging arm linear motion platform 3-2, and the output end of the imaging lens 3-3 is connected with the cubic spectroscope 6.
Further, the sample arm 4 comprises a reflecting mirror 4-2, a silicon chip is arranged on the reflecting mirror 4-2, the reflecting mirror 4-2 is arranged on the sample arm linear motion platform 4-1, a lens of the reflecting mirror 4-2 corresponds to a sample microscope objective 4-3, and the sample microscope objective 4-3 is connected with the cube spectroscope 6.
Further, the reference arm 5 comprises a reference arm linear motion platform 5-1, a reflective mirror 5-2 is arranged on the reference arm linear motion platform 5-1, a silicon chip is arranged on the reflective mirror 5-2, a reference microscope objective 5-3 is correspondingly arranged on a lens of the reflective mirror 5-2, and an output end of the reference microscope objective 5-3 is connected with the cubic beam splitter 6.
Furthermore, the auxiliary adjusting light source 2-1 is an LD point light source, and the central wavelength of the auxiliary adjusting light source is 705nm.
Furthermore, the surface of the reflector 4-2 is provided with local burr scratches.
Further, the collimating lens 2-3 is a single lens with positive focal power or a lens group consisting of two or more lenses.
Further, the image sensor of the CMOS camera 3-1 is an area array detector, which is one of a CCD detector or a CMOS detector.
As shown in fig. 2, the auxiliary debugging method for a parallel optical coherence tomography apparatus according to the present invention includes the following steps:
firstly, a collimating lens 2-3 is a biconvex symmetrical convex lens with a focal length f1 of 50mm, the center of the lens is positioned at the center of a lens body, a vernier caliper is used for roughly positioning the collimating lens 2-3, so that the distance d1 between the collimating lens 2-3 and an auxiliary adjusting light source 2-1 is equal to the focal length f1 of the collimating lens 2-3 of 50mm, and a roughly collimated light beam is obtained;
secondly, the imaging lens 3-3 is an achromatic double cemented lens with a focal length f2 of 150mm, the center of the lens can be regarded as being at the center of the lens body, the distance between the center of the lens and a flange of the CMOS camera 3-1 is d (2-1), the distance between the flange of the CMOS camera 3-1 and the image sensor chip is d (2-2), the distance between the center of the lens and the image sensor chip is d2= d (2-1) + d (2-2), the imaging lens 3-3 is roughly positioned by means of a vernier caliper so that d2= f2=150mm, and an image surface is basically aligned with the image sensor chip of the CMOS camera 3-1 after a light source passes through the collimating lens 2-3 and the imaging lens 3-3;
thirdly, connecting the CMOS camera 3-1 with a display through a collection card and a computer, and checking the point image of the light source through image processing software and the display after the CMOS camera 3-1 images the light source;
fourthly, a light absorption baffle is arranged between the cubic spectroscope 6 and the reference arm 5, and the reference arm 5 is temporarily blocked;
fifthly, driving the collimating lens 2-3 to move by 5um step length by using the lighting arm linear motion platform 2-2, moving the collimating lens 2-3 in any direction by taking the size reduction of the point image as a measurement reference, if the size of the point image is reduced, continuing to move, and if the size of the point image is reduced, moving in the opposite direction, determining the moving direction, then gradually performing micro-movement until the size of the point image is not reduced, and entering the next step;
sixthly, driving the imaging lens 3-3 to move by 5um step length by using the imaging arm linear motion platform 3-2, moving the imaging lens 3-3 in any direction by taking the size reduction of the point image as a measurement reference, if the size of the point image is reduced, continuing to move, otherwise, moving in the opposite direction, determining the moving direction, gradually and slightly moving until the size of the point image is not reduced, and entering the next step;
seventhly, repeating the fifth step and the sixth step for multiple times until the size of the point image is not reduced, stopping the installation and adjustment, and replacing the auxiliary installation and adjustment light source 2-1 with an LED light source;
and an eighth step of moving the mirror 4-2 by 1um step length using the sample arm linear motion platform 4-1 until the scratch or burr on the mirror 4-2 is clearly displayed on the display.
The ninth step, the light absorption baffle between the cube spectroscope 6 and the reference arm 5 is removed, and the light absorption baffle is placed between the cube spectroscope 6 and the sample arm 4;
step ten, moving the silicon wafer on the reflector 4-2 by using the sample arm linear motion platform 4-1 in a step length of 1um until an image on the silicon wafer is clearly displayed on a display;
and step eleven, removing the light absorption baffle, moving the silicon wafer on the reflector 5-2 at a speed of 5 steps/second by using the reference arm linear motion platform 5-1 with a step length of 0.2um, and generating an interference image to finish the whole assembly and adjustment process.
Further, the light path of the adjustment is as follows: the method comprises the steps that light emitted by a light source passes through a single lens or a lens group, a coarse collimated light beam emitted by the light source enters an imaging lens after being transmitted for a certain distance, then enters a camera for imaging, a point image or an object image is displayed by a display, collimation of the light source and calibration of the imaging lens can be completed by utilizing a camera with an uncollimated light source and the uncollimated imaging lens, the light source collimating lens and a focusing lens of the camera are alternately moved respectively according to the point image size or the edge contrast of the object image of the light source on the camera, each step of movement is close to a target with the point image size being reduced or the edge contrast of the object image being increased, and when the point image size reaches a pixel or a minimum image, collimation of the light source and calibration of a camera lens can be completed. In the initial stage of the installation and debugging, the size of a point image or the contrast of the edge of an object image can be observed through human eyes, the multiple times of iterative installation and debugging are carried out by taking the size of the point image or the contrast of the edge of the object image as reference, and in the middle and later stages of the installation and debugging, whether the size of the point image is reduced or whether the contrast of the edge of the object image is increased cannot be distinguished by the human eyes.
The invention provides an auxiliary assembly and adjustment method for parallel optical coherence tomography equipment, which takes a point image or a minimum image of a light source as a reference, uses a collimating lens and an imaging lens to carry out non-reference iterative assembly and adjustment without references such as a collimator tube and the like, directly utilizes the position relation between the collimating lens and the light source and the position relation between the imaging lens and a camera image sensor, can realize interference imaging of a wide-spectrum extended light source through repeated iterative assembly and adjustment, has high assembly and adjustment precision, can greatly improve the assembly and adjustment efficiency of a system, calibrates a camera lens under the condition of no collimator tube, can collimate the light source under the condition of no calibration of the camera lens, and is accurate, reliable and simple and convenient to operate.
It should be emphasized that the embodiments described herein are illustrative rather than restrictive, and thus the present invention is not limited to the embodiments described in the detailed description, but other embodiments derived from the technical solutions of the present invention by those skilled in the art are also within the scope of the present invention.
Claims (3)
1. An auxiliary adjusting method of a parallel optical coherence tomography device is characterized in that: the parallel optical coherence tomography imaging device comprises a cubic spectroscope (6), wherein the input end of the cubic spectroscope (6) is connected with an illumination arm (2), and the output end of the cubic spectroscope (6) is connected with an imaging arm (3), a sample arm (4) and a reference arm (5);
the illumination arm (2) comprises an auxiliary adjusting light source (2-1), the auxiliary adjusting light source (2-1) is connected with a collimating lens (2-3), the collimating lens (2-3) is arranged on the illumination arm linear motion platform (2-2), and the output end of the collimating lens (2-3) is connected with the cubic spectroscope (6);
the imaging arm (3) comprises a CMOS camera (3-1), the CMOS camera (3-1) is correspondingly provided with an imaging lens (3-3), the imaging lens (3-3) is arranged on the imaging arm linear motion platform (3-2), and the output end of the imaging lens (3-3) is connected with the cubic spectroscope (6);
the sample arm (4) comprises a reflecting mirror (4-2), a silicon chip is arranged on the reflecting mirror (4-2), the reflecting mirror (4-2) is arranged on a sample arm linear motion platform (4-1), a lens of the reflecting mirror (4-2) corresponds to a sample microscope objective (4-3), the sample microscope objective (4-3) is connected with the cube spectroscope (6), and the surface of the reflecting mirror (4-2) is provided with local burr scratches;
the reference arm (5) comprises a reference arm linear motion platform (5-1), a reflective mirror (5-2) is arranged on the reference arm linear motion platform (5-1), a silicon wafer is arranged on the reflective mirror (5-2), a lens of the reflective mirror (5-2) corresponds to a reference microscope objective (5-3), and the output end of the reference microscope objective (5-3) is connected with the cube beam splitter (6);
the auxiliary adjusting method of the parallel optical coherence tomography equipment comprises the following steps:
firstly, a collimating lens (2-3) is a biconvex symmetrical convex lens with a focal length f1 of 50mm, the center of the lens is positioned at the center of a lens body, a vernier caliper is used for roughly positioning the collimating lens (2-3), so that the distance d1 between the collimating lens (2-3) and an auxiliary adjusting light source (2-1) is equal to the focal length f1 of the collimating lens (2-3) of 50mm, and a roughly collimated light beam is obtained;
secondly, the imaging lens (3-3) is an achromatic double cemented lens with a focal length f2 of 150mm, the center of the lens is regarded as being at the center of the lens body, the distance between the center of the lens and a flange of the CMOS camera (3-1) is d3, the distance between the flange of the CMOS camera (3-1) and the image sensor chip is d4, the distance between the center of the lens and the image sensor chip is d2= d3+ d4, the imaging lens (3-3) is roughly positioned by means of a vernier caliper so that d2= f2=150mm, and after a light source passes through the collimating lens (2-3) and the imaging lens (3-3), an image plane is basically aligned with the image sensor chip of the CMOS camera (3-1);
thirdly, connecting the CMOS camera (3-1) with a display through a collection card and a computer, and checking the point image of the light source through image processing software and the display after the CMOS camera (3-1) images the light source;
fourthly, a light absorption baffle is placed between the cubic spectroscope (6) and the reference arm (5), and the reference arm (5) is temporarily blocked;
fifthly, driving the collimating lens (2-3) to move by 5um step length by using the lighting arm linear motion platform (2-2), moving the collimating lens (2-3) in any direction by taking the size reduction of the point image as a measurement reference, continuing to move if the size of the point image is reduced, otherwise moving in the opposite direction, determining the moving direction, and then gradually slightly moving until the size of the point image is not reduced any more, and entering the next step;
sixthly, driving the imaging lens (3-3) to move by 5um step length by using the imaging arm linear motion platform (3-2), moving the imaging lens (3-3) in any direction by taking the size reduction of the point image as a measurement reference, continuing to move if the size of the point image is reduced, otherwise moving in the opposite direction, determining the moving direction, and then gradually slightly moving until the size of the point image is not reduced any more, and entering the next step;
seventhly, repeating the fifth step and the sixth step for multiple times until the size of the point image is not reduced, stopping installation and adjustment, and replacing the auxiliary installation and adjustment light source (2-1) with an LED light source;
eighthly, moving the reflector (4-2) by using the sample arm linear motion platform (4-1) in a step length of 1um until scratches or burrs on the reflector (4-2) are clearly displayed on a display;
ninthly, removing a light absorption baffle between the cubic spectroscope (6) and the reference arm (5), and placing the light absorption baffle between the cubic spectroscope (6) and the sample arm (4);
step ten, moving the silicon chip on the reflector (4-2) by 1um step length by using a sample arm linear motion platform (4-1) until an image on the silicon chip is clearly displayed on a display;
and step eleven, removing the light absorption baffle, moving a silicon wafer on the reflector (5-2) at a speed of 5 steps/second by using a reference arm linear motion platform (5-1) with a step length of 0.2um, and generating an interference image to finish the whole assembly and adjustment process.
2. The auxiliary debugging method of the parallel optical coherence tomography equipment according to claim 1, characterized in that: the auxiliary adjusting light source (2-1) is an LD point light source, and the central wavelength of the auxiliary adjusting light source is 705nm.
3. The auxiliary adjusting method of the parallel optical coherence tomography equipment according to claim 1, characterized in that: the collimating lens (2-3) is a single lens with positive focal power or a lens group consisting of two or more lenses.
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