CN111919754B - Potato tissue culture method - Google Patents
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- CN111919754B CN111919754B CN202010973908.6A CN202010973908A CN111919754B CN 111919754 B CN111919754 B CN 111919754B CN 202010973908 A CN202010973908 A CN 202010973908A CN 111919754 B CN111919754 B CN 111919754B
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- 235000002595 Solanum tuberosum Nutrition 0.000 title claims abstract description 140
- 244000061456 Solanum tuberosum Species 0.000 title claims abstract description 140
- 238000012136 culture method Methods 0.000 title claims abstract description 15
- 238000004140 cleaning Methods 0.000 claims abstract description 49
- 230000005540 biological transmission Effects 0.000 claims abstract description 37
- 230000007246 mechanism Effects 0.000 claims abstract description 17
- 239000007788 liquid Substances 0.000 claims description 87
- 238000005192 partition Methods 0.000 claims description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 239000007921 spray Substances 0.000 claims description 21
- 230000001954 sterilising effect Effects 0.000 claims description 21
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 20
- 238000005406 washing Methods 0.000 claims description 19
- 238000004659 sterilization and disinfection Methods 0.000 claims description 18
- 230000033001 locomotion Effects 0.000 claims description 16
- 239000008223 sterile water Substances 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 10
- 230000001360 synchronised effect Effects 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 3
- 238000007790 scraping Methods 0.000 description 12
- 235000012015 potatoes Nutrition 0.000 description 9
- 239000012535 impurity Substances 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 6
- 230000008569 process Effects 0.000 description 5
- 230000000903 blocking effect Effects 0.000 description 4
- 238000011010 flushing procedure Methods 0.000 description 4
- 239000010865 sewage Substances 0.000 description 4
- 206010020649 Hyperkeratosis Diseases 0.000 description 3
- 230000002146 bilateral effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000000265 homogenisation Methods 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 238000004161 plant tissue culture Methods 0.000 description 3
- 230000010405 clearance mechanism Effects 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 210000001938 protoplast Anatomy 0.000 description 2
- 238000007630 basic procedure Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Apparatuses For Bulk Treatment Of Fruits And Vegetables And Apparatuses For Preparing Feeds (AREA)
Abstract
The invention relates to a potato tissue culture method, which uses a potato tissue culture device, wherein the potato tissue culture device comprises a transmission mechanism and a cleaning unit, the lower end of the transmission mechanism is arranged on the existing working ground, the cleaning unit is arranged right below the upper end of the transmission mechanism, and the lower end of the cleaning unit is connected with the existing working ground.
Description
Technical Field
The invention relates to the technical field of plant tissue culture, in particular to a potato tissue culture method.
Background
Tissue culture of plants, also called isolated culture in the broad sense, refers to a technique of separating desired tissues, organs or cells, protoplasts, etc. from plant bodies, and inoculating them on a culture medium containing various nutrients and plant hormones under aseptic conditions by aseptic operation to obtain regenerated whole plants or produce other products having economic value, and the narrow sense refers to a technique of culturing each part of plant tissue, such as cambium, parenchyma, mesophyll tissue, endosperm, etc. to obtain regenerated plants, and also refers to a technique of producing callus from each organ during the culture process, and the callus is redifferentiated to form regenerated plants, and the plant tissue culture can be divided into embryo culture, organ culture, tissue culture, cell culture and protoplast culture, and the basic procedures of plant tissue culture include surface sterilization, callus induction, detoxification and bud differentiation, the basic steps of subculture of buds, rooting culture, hardening off seedlings, transplanting and the like, but the following problems can occur in the surface sterilization process in the potato tissue culture:
1. the device for processing the cut potatoes is suitable for culturing a small amount of potatoes in a single batch, so that the whole working efficiency is low, the time is long, meanwhile, the placing gaps of the potatoes are small, the mutual blocking phenomenon is easy to form, and the phenomenon that part of potatoes are processed unevenly is caused;
2. in the process of washing the potato dices, the washing water flow easily causes the phenomenon that the sewage generated by the previous washing or the residual impurities on the surface of the structure are splashed upwards, so that the probability of secondary pollution of the potato dices is increased, and meanwhile, the potato dices are easily treated unevenly by simply placing the potato dices stacking group in the treatment liquid.
Disclosure of Invention
Technical scheme (I)
In order to achieve the above object, the present invention adopts the following technical solution, wherein a potato tissue culture apparatus is used, the potato tissue culture apparatus comprises a transmission mechanism and a cleaning unit, and the specific culture method when the potato tissue culture apparatus is used for potato tissue culture is as follows:
s1, placing and conveying the potato dices: the potato blocks are manually placed on the placing group, the linkage wheel is driven to do reciprocating rotation motion through the reciprocating unit, and the placing group carries the potato blocks to do synchronous left-right and up-down reciprocating motion through the cooperation among the linkage wheel, the transmission belt and the transmission wheel;
s2, cleaning: the potato blocks sequentially pass through the liquid containing pool from left to right, when the potato blocks are positioned in the liquid containing pool at the leftmost end, cleaning water is injected into the connecting pipe through the cleaning machines at two sides of the liquid containing pool, and the cleaning water is sprayed out by the spray heads to wash the potato blocks;
s3, surface sterilization: the cleaned potato slices sequentially pass through the ethanol solution and the sterilization solution to realize surface sterilization of the potato slices;
s4, washing with sterile water: when the sterilized potato blocks are located in the rightmost liquid containing pool, sterile water is injected into the connecting pipe through the cleaning machines on the two sides of the liquid containing pool, and the sterile water washes the potato blocks through the spray heads.
The lower end of the transmission mechanism is arranged on the existing working ground, the cleaning unit is arranged right below the upper end of the transmission mechanism, and the lower end of the cleaning unit is connected with the existing working ground.
The transmission mechanism comprises an inverted L-shaped frame, shaft fixing plates, shaft rods, a linkage wheel, long vertical plates, short vertical plates, transmission wheels, transmission belts, reciprocating units and a placement group, the lower end of the inverted L-shaped frame is arranged on the existing working floor, the shaft fixing plates are symmetrically arranged on the left and right of the lower end surface of the upper end of the inverted L-shaped frame, the shaft rods are symmetrically arranged on the front and back of the shaft fixing plates, the linkage wheel is arranged on the shaft rods through bearings, the long vertical plates are equidistantly arranged from left to right between the left and right opposite fixed plates, the short vertical plates are symmetrically arranged on the left and right sides of the long vertical plates, the upper end surfaces of the short vertical plates and the upper end surfaces of the long vertical plates are both connected with the lower end surface of the upper end of the inverted L-shaped frame, the transmission wheels are arranged between the lower ends of the front and back opposite short vertical plates and the front and back opposite long vertical plates through pin shafts, and inverted, it is connected with the drive belt to control between the just relative interlocking wheel, link to each other through the sliding fit mode between drive belt and the drive wheel, link to each other through reciprocating unit between the upper end of type of falling L frame and the interlocking wheel, turn right the equidistance from a left side between the drive belt and install and settle the group, place the potato stripping and slicing on settling the group through artifical mode, and make the interlocking wheel be reciprocating rotary motion through reciprocating unit, through interlocking wheel 13, cooperation between drive belt and the drive wheel makes to settle and organizes and carry the potato stripping and slicing and do synchronous left and right sides and up-and-down reciprocating motion, then make the potato stripping and slicing accept the different processing of clearance mechanism in proper order.
The reciprocating unit comprises driven gear teeth, driving gear teeth, a base plate and an electric sliding block, wherein the driven gear teeth are symmetrically arranged at the front end and the rear end of the linkage wheel, the driven gear teeth are positioned on the inner side of the shaft fixing plate, driving gear teeth are arranged above the driven gear teeth from left to right at equal intervals, the driven gear teeth are connected with the driving gear teeth in a sliding fit mode, the upper end surface of the driving gear teeth is connected with the lower end surface of the base plate, the electric sliding block is arranged in the middle of the upper end surface of the base plate, the upper end of the electric sliding block is connected with the upper end of the inverted L-shaped frame in a sliding fit mode, the base plate is driven by the electric sliding block to synchronously reciprocate left and right, the base plate drives the driving gear teeth to synchronously move, the linkage wheel synchronously rotates in a reciprocating mode through the matching between the driven gear teeth and the driving gear teeth, the linkage wheel, the placing group carries the potato blocks to synchronously move along with the transmission belt.
The utility model discloses a potato slicing machine, including T type pole, round platform, fixed plate and three-dimensional square frame, settle the group and include T type pole, the round platform, fixed plate and three-dimensional square frame, T type pole turns right the equidistance from a left side and installs between the drive belt, the lower extreme of T type pole is provided with the round platform, the lower extreme front and back symmetry of round platform is provided with the fixed plate, three-dimensional square frame is installed to the medial surface longitudinal symmetry of just relative fixed plate in front and back, place the potato stripping and slicing in three-dimensional square frame through artifical mode, T type pole drives the round platform and is reciprocal side-to-side motion under the drive of drive belt, simultaneously again because of the existence of V type.
The cleaning unit comprises liquid containing pools, connecting pipes, a cleaning machine and spray heads, wherein the liquid containing pools are arranged on the existing working ground in an equidistance way from left to right, the left end and the right end of the leftmost liquid containing pool and the left end and the right end of the rightmost liquid containing pool are symmetrically provided with the connecting pipes, the lower ends of the connecting pipes are connected with the upper ends of the cleaning machine, the lower ends of the cleaning machine are arranged on the existing working ground, the spray heads are arranged at the inner side ends of the upper ends of the connecting pipes in a threaded fit way, the spray heads are positioned in the liquid containing pools, the liquid containing pools between the liquid containing pools provided with the connecting pipes are sequentially filled with ethanol liquid and sterilizing liquid from left to right, the potato cut blocks are driven by the three-dimensional frame to synchronously do left-right and up-down reciprocating motion, the potato cut blocks sequentially pass through the liquid containing pools from left to right, when the potato cut blocks are positioned in the leftmost liquid containing pools, wash water and wash the potato piece by the shower nozzle blowout to avoid the potato piece to carry impurity such as earth, the potato piece after later washing loops through ethanol liquid and sterilization liquid, in order to realize the surface sterilization of potato piece, when the potato piece is located the flourishing liquid pool of rightmost end, pours into sterile water into the connecting tube into through the cleaning machine of this flourishing liquid pool both sides, and sterile water washes the potato piece through the shower nozzle.
As a preferred technical scheme of the invention, the lower ends of the front and rear inner side walls of the liquid containing tank are symmetrically provided with the ear plates, the ear plates are arranged in a bilateral symmetry way, the pin shafts are arranged between the left and right opposite ear plates, the middle part of the pin shaft is provided with the partition plate in a sliding fit way, the partition plate is positioned below the spray head, the outer side end surface of the partition plate is not contacted with the inner side wall of the liquid containing tank, the outer side end of the upper end surface of the partition plate is symmetrically provided with the electric push rod in a bilateral symmetry way, the upper end of the electric push rod is connected with the inner side wall of the liquid containing tank, when the cleaning machine works for a single time, the partition plate is pushed downwards by the electric push rod until the partition plate is in a horizontal state, at the moment, the front and rear opposite partition plates are matched with the spray head to separate the lower part of the fixed plate, so as, and then guarantee the washing quality of potato stripping and slicing in order to reduce the influence to follow-up cultivation result, after the cleaning machine single work, drive the baffle through electric putter and upwards rotate, the remaining washing sewage of baffle up end can follow the baffle of slope and flow into flourishing liquid pool bottom downwards.
As a preferred technical scheme of the invention, the inner side end of the upper end surface of the partition plate is symmetrically provided with two electric slide blocks in a left-right manner in a sliding fit manner, the two electric slide blocks are positioned between the electric push rods, the upper end surface of the two electric slide blocks is connected with the lower end of the inverted U-shaped plate, the lower end surface of the upper end of the inverted U-shaped plate is provided with scraping brushes equidistantly from left to right, the lower end of the scraping brushes is connected with the upper end surface of the partition plate in a sliding fit manner, after the single work of the cleaning machine is finished, the partition plate is driven to rotate upwards by the electric push rods, the inverted U-shaped plate is driven to move downwards along the partition plate by the two electric slide blocks, the inverted U-shaped plate drives the scraping brushes to move downwards synchronously, the scraping brushes can play a role in scraping residual flushing impurities on the upper end surface of the partition plate so as to avoid the phenomenon that the residual impurities splash upwards, thereby improving the processing quality of the potato dices to improve the success rate of the subsequent cultivation.
According to a preferred technical scheme, the upper end of the circular table is connected with the lower end of the T-shaped rod in a threaded fit mode, a circular groove is formed in the lower end face of the circular table, arc electric sliding blocks are symmetrically arranged in the circular groove in a front-back sliding fit mode, the lower end of each arc electric sliding block is connected with the upper end of the fixing plate, when potato slices are located in the liquid containing pool, the fixing plates are driven to do circular motion along the circular grooves through the arc electric sliding blocks, the fixing plates drive the potato slices to synchronously rotate through the three-dimensional frames, the purpose of improving the uniformity of washing of the potato slices is achieved, meanwhile, the ethanol liquid and the sterilizing liquid can form a vortex state to improve the contact degree and uniformity of the potato slices and the two liquid, and further the overall processing quality of the potato slices is improved.
As a preferred technical scheme of the invention, the side end faces of the three-dimensional frames are all of a grid structure, the three-dimensional frames opposite to each other in the front and back are in an up-and-down staggered arrangement structure, the grid structure can realize water permeability and can realize the functions of separating and preventing falling, and the up-and-down staggered arrangement structure can reduce the probability of mutual blocking phenomenon formed among the potato blocks on the basis of realizing the processing of a plurality of potato blocks, thereby improving the homogenization degree of the processing of the potato blocks.
As a preferred technical scheme of the invention, the connecting plates are arranged between the adjacent T-shaped rods, the lower end surfaces of the connecting plates are symmetrically provided with the convex structures at the left and right sides, the convex structures are connected with the upper ends of the liquid containing pools in a sliding fit manner, and when the three-dimensional frame carries the potato cut blocks to move from the current liquid containing pool to the next liquid containing pool, the connecting plates can play a role in receiving the dripping treatment liquid at the middle transition section, so that the probability of pollution of the dripping treatment liquid to the liquid in the liquid containing pools is reduced, and meanwhile, the probability of pollution to the working environment is reduced.
According to a preferable technical scheme of the invention, the telescopic plates are symmetrically arranged at the upper and lower sides of the inner sides of the fixed plates which are opposite from front to back, the telescopic plates are connected with the upper end of the three-dimensional frame in a sliding fit manner, the telescopic plates are symmetrically arranged at the front and back, through holes are formed in the upper end surfaces of the telescopic plates at equal intervals from front to back, the through holes are arranged at equal intervals from left to right, the potato cut pieces are placed in the three-dimensional frame in a manual manner, the telescopic plates are pulled out in a manual manner to enable the upper end of the three-dimensional frame to be in a certain closed state, and the potato cut pieces can be limited by the fit between the telescopic plates and the three-dimensional frame, so that the potato cut pieces are prevented from floating upwards and being separated from the three-dimensional frame when being immersed in.
(II) advantageous effects
1. According to the potato tissue culture method, the potato tissue culture is carried out by adopting the design concept of a multi-station treatment structure, the arranged transmission mechanism can realize synchronous, orderly and gradual surface sterilization treatment of the cleaning mechanism for a plurality of potato blocks, so that the potato block culture treatment efficiency can be greatly improved, the labor can be saved, and meanwhile, a structure with a protection effect is arranged aiming at the washing of the potato blocks so as to improve the washing quality of the potatoes;
2. the partition plate and the spray head are matched to partition the lower part of the fixed plate, so that the phenomenon that sewage generated after washing the cut potatoes stored in the liquid containing pool splashes upwards to cause repeated pollution to the potatoes is avoided, and the washing quality of the cut potatoes is further ensured to reduce the influence on the subsequent culture result;
3. the scraping brush can play a role in scraping residual flushing impurities on the upper end surface of the partition plate so as to avoid the phenomenon that the residual impurities jump upwards along with water drops in subsequent flushing to pollute the cut potatoes, and further improve the processing quality of the cut potato receiving so as to improve the success rate of subsequent culture;
4. according to the invention, the circular arc electric sliding block and the fixing plate are matched, so that the potato cut pieces are subjected to treatment in a rotating state, the uniformity of washing the potato cut pieces is improved, and meanwhile, the ethanol solution and the sterilization solution form a vortex shape, so that the contact uniformity of the potato cut pieces and the potato cut pieces is improved, and the integral treatment quality of the potato cut pieces is improved;
5. the three-dimensional square frame with the structure of staggered arrangement from top to bottom can reduce the probability of mutual blocking phenomenon formed among the potato blocks on the basis of realizing the processing of a plurality of potato blocks, thereby improving the homogenization degree of the processing of the potato blocks.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a process flow diagram of the present invention;
FIG. 2 is a schematic perspective view of the present invention;
FIG. 3 is a first cross-sectional view of the present invention;
FIG. 4 is a second cross-sectional view of the present invention;
FIG. 5 is an enlarged view of the X-direction detail of FIG. 3 of the present invention;
FIG. 6 is an enlarged view of the Y-direction portion of FIG. 4 in accordance with the present invention;
FIG. 7 is an enlarged view of the invention in the Z-direction of FIG. 4;
fig. 8 is an enlarged view of the invention in the direction of M of fig. 4.
Detailed Description
The embodiments of the invention will be described in detail below with reference to the drawings, but the invention can be implemented in many different ways, which are defined and covered by the claims.
As shown in fig. 1 to 8, a potato tissue culture method using a potato tissue culture apparatus comprising a transmission mechanism 1 and a cleaning unit 2 includes the following specific culture methods:
s1, placing and conveying the potato dices: the potato blocks are manually placed on the placing group, the linkage wheel 13 is driven to do reciprocating rotation motion through the reciprocating unit, and the placing group carries the potato blocks to do synchronous left-right and up-down reciprocating motion through the cooperation among the linkage wheel 13, the transmission belt 17 and the transmission wheel 16;
s2, cleaning: the potato blocks sequentially pass through the liquid containing pool 20 from left to right, when the potato blocks are positioned in the liquid containing pool 20 at the leftmost end, cleaning water is injected into the connecting pipe 21 through the cleaning machines 22 at the two sides of the liquid containing pool 20, and the cleaning water is sprayed out from the spray head 23 to wash the potato blocks;
s3, surface sterilization: the cleaned potato slices sequentially pass through the ethanol solution and the sterilization solution to realize surface sterilization of the potato slices;
s4, washing with sterile water: when the sterilized potato blocks are positioned in the liquid containing pool 20 at the rightmost end, sterile water is injected into the connecting pipe 21 through the cleaning machines 22 at the two sides of the liquid containing pool 20, and the sterile water washes the potato blocks through the spray nozzles 23.
The lower end of the transmission mechanism 1 is arranged on the existing working ground, the cleaning unit 2 is arranged right below the upper end of the transmission mechanism 1, and the lower end of the cleaning unit 2 is connected with the existing working ground.
The transmission mechanism 1 comprises an inverted L-shaped frame 10, a shaft fixing plate 11, a shaft rod 12, a linkage wheel 13, a long vertical plate 14, a short vertical plate 15, a transmission wheel 16, a transmission belt 17, a reciprocating unit and a placement group, wherein the lower end of the inverted L-shaped frame 10 is arranged on the existing working ground, the shaft fixing plate 11 is symmetrically arranged on the left and right of the lower end surface of the upper end of the inverted L-shaped frame 10, the shaft fixing plate 11 is symmetrically arranged in front and back, the shaft rod 12 is arranged between the lower ends of the front and back shaft fixing plates 11 which are opposite, the linkage wheel 13 is arranged on the shaft rod 12 through a bearing, the long vertical plates 14 are equidistantly arranged between the left and right fixed plates 192 which are opposite, the short vertical plates 15 are symmetrically arranged on the left and right sides of the long vertical plates 14, the upper end surfaces of the short vertical plates 15 and the upper end surfaces of the long vertical plates 14 are both connected with the lower end surface of the upper end of the inverted, an inverted V-shaped structure is formed between a driving wheel 16 at the lower end of a long vertical plate 14 and a driving wheel 16 at the lower end of a short vertical plate 15 adjacent to the long vertical plate, a driving belt 17 is connected between the right and left linkage wheels 13 which are opposite, the driving belt 17 is connected with the driving wheel 16 in a sliding fit mode, the upper end of an inverted L-shaped frame 10 is connected with the linkage wheels 13 through reciprocating units, arrangement groups are arranged between the driving belts 17 at equal intervals from left to right, after potato blocks are manually placed on the arrangement groups, the linkage wheels 13 make reciprocating rotation movement through the reciprocating units, the linkage wheels 13 drive the driving belt 17 to move synchronously, the driving wheel 16 makes reciprocating rotation movement synchronously along with the linkage wheels 13 through the driving belt 17, the arrangement groups carry the potato blocks to move synchronously along with the potato blocks 17, and due to the existence of the inverted V-shaped structure, the arrangement groups can make reciprocating movement from, make the potato stripping and slicing accept the different processing of clearance mechanism in proper order then, the device is whole can realize the synchronous orderly processing of polylith potato stripping and slicing simultaneously, and then can improve potato stripping and slicing cultivation treatment effeciency greatly and can practice thrift the manpower.
The reciprocating unit comprises driven gear teeth 180, driving gear teeth 181, a base plate 182 and a first electric sliding block 183, wherein the driven gear teeth 180 are symmetrically arranged at the front end and the rear end of the linkage wheel 13, the driven gear teeth 180 are positioned on the inner side of the fixed shaft plate 11, the driving gear teeth 181 are arranged above the driven gear teeth 180 from left to right at equal intervals, the driven gear teeth 180 are connected with the driving gear teeth 181 in a sliding fit manner, the upper end surface of the driving gear teeth 181 is connected with the lower end surface of the base plate 182, the first electric sliding block 183 is arranged in the middle of the upper end surface of the base plate 182, the upper end of the first electric sliding block 183 is connected with the upper end of the inverted L-shaped frame 10 in a sliding fit manner, the base plate 182 is driven by the first electric sliding block 183 to synchronously reciprocate left and right, the base plate 182 drives the driving gear teeth 181 to synchronously move, the driven gear teeth 180 are matched, the linkage wheel 13 drives the transmission belt 17 to move synchronously, the transmission wheel 16 synchronously makes reciprocating rotation motion along with the linkage wheel 13 through the transmission belt 17, and the placing group carries the potato blocks to move synchronously along with the transmission belt 17.
The arrangement group comprises a T-shaped rod 190, round platforms 191, fixing plates 192 and a three-dimensional frame 193, the T-shaped rod 190 is arranged between the transmission belts 17 in an equidistance mode from left to right, the round platforms 191 are arranged at the lower end of the T-shaped rod 190, the fixing plates 192 are symmetrically arranged at the front and back of the lower end of the round platforms 191, the three-dimensional frame 193 is symmetrically arranged at the upper and lower sides of the inner side faces of the fixing plates 192 opposite to each other in a front-back mode, potato blocks are manually placed in the three-dimensional frame 193, the T-shaped rod 190 drives the round platforms 191 to reciprocate left and right under the driving of the transmission belts 17, meanwhile, the T-shaped rod 190 synchronously reciprocates up and down due to the existence of a V-shaped structure, the.
The upper end of the circular truncated cone 191 is connected with the lower end of the T-shaped rod 190 through a thread fit mode, a circular groove is formed in the lower end face of the circular truncated cone 191, circular arc electric sliders 19a are symmetrically arranged in the circular groove in a front-back mode through a sliding fit mode, the lower end of each circular arc electric slider 19a is connected with the upper end of the corresponding fixed plate 192, when potato slices are located in the liquid containing pool 20, the circular arc electric sliders 19a drive the fixed plates 192 to do circular motion along the circular groove, the fixed plates 192 drive the potato slices to synchronously rotate through the three-dimensional frame 193, the purpose of improving the uniformity of washing of the potato slices is achieved, meanwhile, the ethanol liquid and the sterilization liquid can form a vortex state, the contact degree and the uniformity of the potato slices and the two potato slices are improved, and the overall processing quality of the potato slices is further.
The inner sides of the fixing plates 192 which are right opposite to each other in the front and back are symmetrically provided with the telescopic plates 19c up and down, the telescopic plates 19c are connected with the upper end of the three-dimensional frame 193 in a sliding fit mode, the telescopic plates 19c are symmetrically arranged in the front and back, through holes are formed in the upper end faces of the telescopic plates 19c in the front and back at equal intervals, the through holes are arranged in the left and right at equal intervals, after the potato cut pieces are placed in the three-dimensional frame 193 in a manual mode, the telescopic plates 19c are pulled out in a manual mode to enable the upper end of the three-dimensional frame 193 to be in a certain closed state, the potato cut pieces can be limited by the cooperation between the telescopic plates 19c and the three-dimensional frame 193, and therefore the potato cut pieces are prevented from floating up and being separated from the three-dimensional frame.
The side end faces of the three-dimensional square frames 193 are all of grid structures, the three-dimensional square frames 193 which are opposite from front to back are in an up-and-down staggered arrangement structure, the grid structures can achieve the functions of water permeability and separation and anti-falling, the up-and-down staggered arrangement structure can reduce the probability of mutual blocking phenomenon formed among the potato blocks on the basis of achieving that a plurality of potato blocks are subjected to treatment, and further the homogenization degree of the potato block treatment is improved.
Adjacent T type pole 190 between be provided with fishplate bar 19b, fishplate bar 19 b's lower terminal surface bilateral symmetry installs protruding structure, protruding structure passes through the sliding fit mode and links to each other with the upper end of flourishing liquid pool 20, when three-dimensional square frame 193 carries the potato stripping and slicing and removes the in-process by present flourishing liquid pool 20 to next flourishing liquid pool 20, fishplate bar 19b can play the effect of accepting the processing liquid that drips in the middle changeover portion, and then reduced the probability that the processing liquid that drips led to the fact the pollution to the inside liquid of flourishing liquid pool 20, can reduce the probability that causes the pollution to operational environment simultaneously again.
The cleaning unit 2 comprises liquid containing pools 20, connecting pipes 21, a cleaning machine 22 and spray heads 23, wherein the liquid containing pools 20 are arranged on the existing working ground in a left-to-right equidistant mode, the left end and the right end of the leftmost liquid containing pool 20 and the left end and the right end of the rightmost liquid containing pool 20 are symmetrically provided with the connecting pipes 21, the lower ends of the connecting pipes 21 are connected with the upper ends of the cleaning machine 22, the lower ends of the cleaning machine 22 are arranged on the existing working ground, the spray heads 23 are arranged at the inner side ends of the upper ends of the connecting pipes 21 in a threaded matching mode, the spray heads 23 are positioned in the liquid containing pools 20, the liquid containing pools 20 among the liquid containing pools 20 provided with the connecting pipes 21 are sequentially filled with ethanol liquid and sterilizing liquid from left to right, in the process that the potato cutting blocks are driven by a three-dimensional frame 193 to synchronously reciprocate left and right and up and down, the potato cutting blocks sequentially pass through the liquid containing pools 20 from right, when the, wash water is injected into connecting pipe 21 through the cleaning machine 22 of this flourishing liquid bath 20 both sides, wash water and wash the potato dice by shower nozzle 23 blowout, in order to avoid the potato dice to carry impurity such as earth, potato dice after later washing loops through ethanol liquid and sterilization liquid, in order to realize the surface sterilization of potato dice, when the potato dice is located the flourishing liquid bath 20 of right-hand member, inject aseptic water into connecting pipe 21 through the cleaning machine 22 of this flourishing liquid bath 20 both sides, aseptic water washes the potato dice through shower nozzle 23.
The lower ends of the front inner side wall and the rear inner side wall of the liquid containing tank 20 are symmetrically provided with the ear plates 201, the ear plates 201 are symmetrically arranged in a left-right mode, a pin shaft is arranged between the left ear plate 201 and the right ear plate 201 which are opposite, the middle part of the pin shaft is provided with the partition plate 202 in a sliding fit mode, the partition plate 202 is positioned below the spray head 23, the outer end surface of the partition plate 202 is not contacted with the inner side wall of the liquid containing tank 20, the outer end surface of the upper end surface of the partition plate 202 is symmetrically provided with the electric push rod 203 in a left-right mode, the upper end of the electric push rod 203 is connected with the inner side wall of the liquid containing tank 20, when the cleaning machine 22 works for a single time, the partition plate 202 is pushed downwards through the electric push rod 203 until the partition plate 202 is in a horizontal state, at the moment, the front and rear baffle plate 202 and the spray head 23 which are opposite are, and then guarantee the washing quality of potato stripping and slicing in order to reduce the influence to follow-up cultivation result, after cleaning machine 22 single work, drive baffle 202 through electric putter 203 and upwards rotate, the remaining washing sewage that the baffle 202 up end can follow the baffle 202 of slope and flow into flourishing liquid pond 20 bottom downwards.
The inner side end of the upper end face of the partition plate 202 is bilaterally and symmetrically provided with two electric sliding blocks 20a in a sliding fit mode, the two electric sliding blocks 20a are positioned between the electric push rods 203, the upper end face of the two electric sliding blocks 20a is connected with the lower end of the inverted U-shaped plate 20b, the lower end face of the upper end of the inverted U-shaped plate 20b is provided with scraping brushes 20c in an equal distance from left to right, the lower end of the scraping brush 20c is connected with the upper end face of the partition plate 202 in a sliding fit mode, after the single work of the cleaning machine 22 is finished, the partition plate 202 is driven to rotate upwards through the electric push rods 203, meanwhile, the inverted U-shaped plate 20b is driven to move downwards along the partition plate 202 through the two electric sliding blocks 20a, the inverted U-shaped plate 20b drives the scraping brushes 20c to move downwards synchronously, the scraping brushes 20c can play a role in scraping residual flushing impurities from the upper end face of the partition plate 202, so as to avoid the phenomenon, thereby improving the processing quality of the potato dices to improve the success rate of the subsequent cultivation.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (7)
1. A potato tissue culture method uses a potato tissue culture device which comprises a transmission mechanism (1) and a cleaning unit (2), and is characterized in that the specific culture method when the potato tissue culture device is used for carrying out potato tissue culture is as follows:
s1, placing and conveying the potato dices: the potato blocks are manually placed on the placing group, the linkage wheel (13) is driven to do reciprocating rotation motion through the reciprocating unit, and the placing group carries the potato blocks to do synchronous left-right and up-down reciprocating motion through the cooperation among the linkage wheel (13), the transmission belt (17) and the transmission wheel (16);
s2, cleaning: the potato blocks sequentially pass through the liquid containing pool (20) from left to right, when the potato blocks are positioned in the liquid containing pool (20) at the leftmost end, cleaning water is injected into the connecting pipe (21) through the cleaning machines (22) at the two sides of the liquid containing pool (20), and the cleaning water is sprayed out by the spray nozzle (23) to wash the potato blocks;
s3, surface sterilization: the cleaned potato slices sequentially pass through the ethanol solution and the sterilization solution to realize surface sterilization of the potato slices;
s4, washing with sterile water: when the sterilized potato dices are positioned in the liquid containing pool (20) at the rightmost end, sterile water is injected into the connecting pipe (21) through the cleaning machines (22) at the two sides of the liquid containing pool (20), and the potato dices are washed by the sterile water through the spray head (23);
the lower end of the transmission mechanism (1) is arranged on the existing working ground, a cleaning unit (2) is arranged right below the upper end of the transmission mechanism (1), and the lower end of the cleaning unit (2) is connected with the existing working ground;
the transmission mechanism (1) comprises an inverted L-shaped frame (10), shaft fixing plates (11), shaft rods (12), linkage wheels (13), long vertical plates (14), short vertical plates (15), transmission wheels (16), transmission belts (17), reciprocating units and arrangement groups, wherein the lower ends of the inverted L-shaped frame (10) are installed on the existing working ground, the shaft fixing plates (11) are symmetrically installed on the left and right of the lower end face of the upper end of the inverted L-shaped frame (10), the shaft fixing plates (11) are symmetrically arranged in the front and back direction, the shaft rods (12) are installed between the lower ends of the front and back oppositely-facing shaft fixing plates (11), the linkage wheels (13) are installed on the shaft rods (12) through bearings, the long vertical plates (14) are equidistantly arranged between the left and right oppositely-facing fixed plates (192) from left to right, the short vertical plates (15) are symmetrically arranged on the left and right sides of the long vertical plates (14), the upper end faces of the short vertical plates (15) and the upper end faces of, the lower ends of front and rear short vertical plates (15) and the lower ends of front and rear long vertical plates (14) which are right opposite are respectively provided with a driving wheel (16) through a pin shaft, the driving wheel (16) at the lower end of the long vertical plate (14) and the driving wheel (16) at the lower end of the short vertical plate (15) adjacent to the driving wheel form an inverted V-shaped structure, a driving belt (17) is connected between left and right linkage wheels (13) which are right opposite, the driving belt (17) is connected with the driving wheel (16) in a sliding fit manner, the upper end of an inverted L-shaped frame (10) is connected with the linkage wheels (13) through a reciprocating unit, and arranging groups are arranged between the driving belts (17) at equal;
the reciprocating unit comprises driven gear teeth (180), driving gear teeth (181), a base plate (182) and an electric sliding block (183), wherein the driven gear teeth (180) are symmetrically arranged at the front end and the rear end of a linkage wheel (13), the driven gear teeth (180) are positioned on the inner side of a shaft fixing plate (11), the driving gear teeth (181) are arranged above the driven gear teeth (180) from left to right at equal intervals, the driven gear teeth (180) are connected with the driving gear teeth (181) in a sliding fit manner, the upper end face of the driving gear teeth (181) is connected with the lower end face of the base plate (182), the electric sliding block (183) is arranged in the middle of the upper end face of the base plate (182), and the upper end of the electric sliding block (183) is connected with the upper end of the inverted L-shaped frame (10) in a;
the arrangement group comprises T-shaped rods (190), round tables (191), fixing plates (192) and three-dimensional frames (193), the T-shaped rods (190) are equidistantly arranged between the transmission belts (17) from left to right, the round tables (191) are arranged at the lower ends of the T-shaped rods (190), the fixing plates (192) are symmetrically arranged at the lower ends of the round tables (191) front and back, and the three-dimensional frames (193) are symmetrically arranged at the upper and lower parts of the inner side end faces of the fixing plates (192) which are right opposite to each other front and back;
the cleaning unit (2) comprises liquid containing pools (20), connecting pipes (21), a cleaning machine (22) and spray heads (23), wherein the liquid containing pools (20) are arranged on the existing working ground in a right-to-right equidistant mode from left, the connecting pipes (21) are symmetrically arranged at the left end and the right end of the liquid containing pool (20) at the leftmost end and the left end and the right end of the liquid containing pool (20) at the rightmost end, the lower ends of the connecting pipes (21) are connected with the upper ends of the cleaning machine (22), the lower ends of the cleaning machine (22) are arranged on the existing working ground, the spray heads (23) are arranged at the inner side ends of the upper ends of the connecting pipes (21) in a threaded matching mode, the spray heads (23) are located inside the liquid containing pools (20), and the liquid containing pools (20) between the liquid containing pools (20) provided with the connecting pipes (21) are sequentially filled with.
2. The potato tissue culture method according to claim 1, wherein the lower ends of the front and rear inner side walls of the liquid containing tank (20) are symmetrically provided with the ear plates (201), the ear plates (201) are symmetrically arranged in a left-right manner, the pin shafts are arranged between the right and left ear plates (201) which are opposite, the middle parts of the pin shafts are provided with the partition plates (202) in a sliding fit manner, the partition plates (202) are positioned below the spray heads (23), the outer side end surfaces of the partition plates (202) are not in contact with the inner side walls of the liquid containing tank (20), the outer side end surfaces of the upper end surfaces of the partition plates (202) are symmetrically provided with the electric push rods (203), and the upper ends of the electric push rods (203) are connected with the inner side walls of the liquid containing.
3. The potato tissue culture method according to claim 2, wherein two electric sliders (20a) are symmetrically arranged on the inner side end of the upper end face of the partition plate (202) in a left-right mode in a sliding fit manner, the two electric sliders (20a) are located between the electric push rods (203), the upper end face of the two electric sliders (20a) is connected with the lower end of the inverted U-shaped plate (20b), scrapers (20c) are arranged on the lower end face of the upper end of the inverted U-shaped plate (20b) in an equidistant mode from left to right, and the lower end of each scraper (20c) is connected with the upper end face of the partition plate (202) in a sliding fit manner.
4. The potato tissue culture method according to claim 1, wherein the upper end of the circular truncated cone (191) is connected with the lower end of the T-shaped rod (190) in a threaded fit manner, a circular groove is formed in the lower end surface of the circular truncated cone (191), circular arc electric sliders (19a) are symmetrically arranged in the circular groove in a front-back manner in a sliding fit manner, and the lower end of each circular arc electric slider (19a) is connected with the upper end of the fixing plate (192).
5. The method for culturing potato tissue according to claim 1, wherein the side end faces of the three-dimensional frames (193) are all of a grid structure, and the three-dimensional frames (193) which are opposite to each other are arranged in a staggered manner.
6. The potato tissue culture method according to claim 1, wherein a joint plate (19b) is arranged between the adjacent T-shaped rods (190), and the lower end surface of the joint plate (19b) is symmetrically provided with a convex structure at left and right sides, and the convex structure is connected with the upper end of the liquid containing pool (20) in a sliding fit manner.
7. The potato tissue culture method according to claim 1, wherein the inner sides of the front and back fixed plates (192) which are opposite to each other are symmetrically provided with telescopic plates (19c) up and down, the telescopic plates (19c) are connected with the upper end of the three-dimensional frame (193) in a sliding fit manner, the telescopic plates (19c) are symmetrically arranged in front and back, the upper end surfaces of the telescopic plates (19c) are provided with through holes at equal intervals from front to back, and the through holes are arranged at equal intervals from left to right.
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