CN111888278B - Hair-growing oil and preparation method thereof - Google Patents

Abstract

The invention provides a hair tonic oil prepared from raw materials comprising the following components: 45-50wt% geranic acid, 25-32wt% choline, 20-25wt% PBS buffer and 0.001-0.1wt% keratinocyte growth factor. In the present invention, the ratio of geranic acid and choline is adjusted, and the two are used as the carrier of keratinocyte growth factor, which can increase the permeability of the skin, and cooperate with PBS buffer to provide a relatively stable ionic environment, so that keratinocytes can be Growth factors penetrate the skin and ensure their activity, further stimulating the proliferation and differentiation of hair follicle stem cells and hair matrix cells, thereby promoting the regeneration and maturation of hair follicles and achieving hair growth. The results of the examples show that when the hair tonic provided by the present invention is evenly smeared on the skin of adult black mice whose back hair has been shaved, most of the shaved parts return to the length and density before being shaved within three weeks.

Description

A kind of hair growth oil and preparation method thereof

technical field

The invention belongs to the technical field of daily cosmetics, and particularly relates to a hair tonic and a preparation method thereof.

Background technique

Seborrheic alopecia, the scientific name is androgenetic alopecia, accounts for about 90% of hair loss patients. The speed and range of hair loss in seborrheic alopecia vary from person to person. Some only have mild baldness, or can last for many years. It can also reach the level of senile baldness in just a few years, and generally progresses fastest around the age of 30. In our country, anti-hair loss products are included in the scope of special product production license management. This series of reasons has led to slow progress in research on hair loss in the past few decades.

The current drug for hair loss is minoxidil liniment, which is applied to the affected area of the head. After being absorbed by the scalp, it can expand the blood vessels under the scalp, improve the microcirculation around the hair follicle, reduce the infiltration of inflammatory cells around the hair follicle, and delay the epithelium. Senescence of stromal cells, which in turn enlarges shrinking hair follicles and promotes hair growth. However, this liniment topical drug needs to be administered through the skin, and the epidermis, especially the stratum corneum, is the main barrier layer of the skin. Studies have found that only a few drugs can enter the skin, and the stability is poor, and the biological activity is difficult to maintain for a long time, resulting in This kind of liniment is difficult to play a good hair growth effect.

Therefore, there is an urgent need to improve hair growth products to provide their hair growth effects.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a hair tonic and a preparation method thereof. The hair growth oil provided by the present invention has excellent hair growth effect.

The invention provides a hair tonic, which is prepared from raw materials comprising the following components: 45wt%-50wt% geranic acid, 25wt%-32wt% choline, 20wt%-25wt% PBS buffer and 0.001wt%-0.1wt% % Keratinocyte Growth Factor.

Preferably, it is prepared from raw materials including the following components: 48wt%-50wt% geranic acid, 29wt%-32wt% choline, 22wt%-25wt% PBS buffer and 0.001wt%-0.05wt% keratinocyte growth factor.

Preferably, it is prepared from raw materials including the following components: 48.192wt%～48.197wt% geranic acid, 29.075wt%～29.077wt% choline, 22.723wt%～22.725wt% PBS buffer and 0.001wt%～0.005 wt% keratinocyte growth factor.

Preferably, the purity of the geranic acid is ≥85%.

The present invention also provides the preparation method of the hair growth oil described in the above technical solution, comprising the following steps:

(1) geranic acid and choline are mixed to carry out metathesis reaction to obtain ionic liquid;

(2) drying the ionic liquid obtained in the step (1) to obtain an oily liquid;

(3) mixing keratinocyte growth factor and PBS buffer to obtain growth factor solution;

(4) mixing the oily liquid obtained in the step (2) with the growth factor solution obtained in the step (3) to obtain hair growth oil;

The steps (1)-(2) and step (3) are not in order.

Preferably, the mixing in the step (1) is carried out under stirring conditions.

Preferably, the end point of the metathesis reaction in the step (1) is the generation of no carbon dioxide gas.

Preferably, the drying temperature in the step (2) does not exceed 70°C.

Preferably, the drying temperature in the step (2) is 50-60°C.

Preferably, the drying time in the step (2) is not less than 36h.

The invention provides a hair tonic oil prepared from raw materials comprising the following components: 45wt%-50wt% geranic acid, 25wt%-32wt% choline, 20wt%-25wt% PBS buffer and 0.001wt% % ~ 0.1 wt% keratinocyte growth factor. The invention can increase the permeability of the skin by adjusting the ratio of geranic acid and choline as carriers of keratinocyte growth factor, and cooperate with PBS buffer to provide a relatively stable ionic environment, so that the original The macromolecular keratinocyte growth factor that cannot penetrate the skin penetrates the skin and ensures its activity, further stimulating the proliferation and differentiation of hair follicle stem cells and hair matrix cells, thereby promoting the regeneration and maturation of hair follicles and achieving hair growth effect. The results of the examples show that when the hair tonic provided by the present invention is evenly smeared on the skin of adult black mice whose back hair has been shaved, most of the shaved parts are restored to the length and density before being shaved within three weeks.

Description of drawings

Figure 1 is a comparison chart of black mice without applying the product of control group 1 and applying product of control group 1 for 7 days, 14 days and 21 days;

Figure 2 is a comparison chart of black mice without applying the product of the control group 2 and applying the product of the control group 2 for 7 days, 14 days and 21 days;

Figure 3 is a comparison chart of black mice without applying the 3 products of the control group and applying the 3 products of the control group for 7 days, 14 days and 21 days;

Figure 4 is a comparison chart of black mice without applying the product of Example 1 and applying the product of Example 1 for 7 days, 14 days and 21 days.

Detailed ways

The invention provides a hair tonic, which is prepared from raw materials comprising the following components: 45wt%-50wt% geranic acid, 25wt%-32wt% choline, 20wt%-25wt% PBS buffer and 0.001wt%-0.1wt% % Keratinocyte Growth Factor.

The raw material of the hair growth oil provided by the present invention includes 45wt% to 50wt% of geranic acid, preferably 48wt% to 50wt%, more preferably 48.192wt% to 48.197wt%, more preferably 48.195wt%. In the present invention, the purity of the geranic acid is preferably ≥85%. In the present invention, when the purity of the geranic acid is less than 85%, the present invention preferably purifies the geranic acid. The present invention has no particular limitation on the purification operation, and the purity of geranic acid can be increased to more than 85%. The source of the geranic acid is not particularly limited in the present invention, and a commercially available product well known to those skilled in the art can be used. In the present invention, the geranic acid can undergo a metathesis reaction with choline to obtain an ionic liquid, which can promote the skin permeability of keratinocyte growth factor (KGF-2) and improve the hair growth effect.

The raw material of the hair tonic provided by the present invention includes 25wt% to 32wt% of choline, preferably 29wt% to 32wt%, more preferably 29.075wt% to 29.077wt%, more preferably 29.076wt%. The source of the choline is not particularly limited in the present invention, and a commercially available product well known to those skilled in the art can be used. In the present invention, the choline can undergo metathesis reaction with geranic acid to obtain ionic liquid, which can promote the skin permeability of KGF-2 and improve the hair growth effect.

The raw material of the hair tonic provided by the present invention includes 20wt% to 25wt% of PBS buffer, preferably 22wt% to 25wt%, more preferably 22.723wt% to 22.725wt%, more preferably 22.724wt%. The source of the PBS buffer is not particularly limited in the present invention, and commercially available products well known to those skilled in the art can be used. In the present invention, the PBS buffer can not only dissolve KGF-2, but also provide a relatively stable ionic environment to avoid inactivation of KGF-2 and protect KGF-2.

The raw material of the hair tonic provided by the present invention includes keratinocyte growth factor 0.001wt%-0.1wt%, preferably 0.001wt%-0.05wt%, more preferably 0.001wt%-0.005wt%, more preferably 0.003wt%. The source of the keratinocyte growth factor is not particularly limited in the present invention, and a commercially available product well known to those skilled in the art can be used. In the present invention, the keratinocyte growth factor can stimulate the proliferation and differentiation of hair follicle stem cells and hair matrix cells, thereby promoting the regeneration and maturation of hair follicles, and achieving the effect of hair growth.

The invention can increase the permeability of the skin by adjusting the ratio of geranic acid and choline as carriers of keratinocyte growth factor, and cooperate with PBS buffer to provide a relatively stable ionic environment, so that the original The macromolecular keratinocyte growth factor that cannot penetrate the skin penetrates the skin and ensures its activity, further stimulating the proliferation and differentiation of hair follicle stem cells and hair matrix cells, thereby promoting the regeneration and maturation of hair follicles and achieving hair growth effect.

The invention overcomes the shortcoming of keratinocyte growth factor action time by adjusting the ratio of raw materials, can stimulate the growth of epithelial cells, start the coordinated signal feedback of epithelial cells to the subcutaneous mesenchyme, promote the formation of new tissues, and improve the The hair growth oil of the present invention can reduce the immunogenicity in the body, reduce the incidence of adverse reactions such as allergies, has no toxicity, no potential side effects, and has high safety; it can stimulate the growth of new or aged epithelial cells. The role of splitting has a wide range of applications.

The present invention also provides the preparation method of the hair growth oil described in the above technical solution, comprising the following steps:

(1) geranic acid and choline are mixed to carry out metathesis reaction to obtain ionic liquid;

(2) drying the ionic liquid obtained in the step (1) to obtain an oily liquid;

(3) mixing keratinocyte growth factor and PBS buffer to obtain growth factor solution;

(4) mixing the oily liquid obtained in the step (2) with the growth factor solution obtained in the step (3) to obtain hair growth oil;

The steps (1)-(2) and step (3) are not in order.

In the present invention, geranic acid and choline are mixed to carry out metathesis reaction to obtain ionic liquid. In the present invention, the mixing of geranic acid and choline is preferably carried out under stirring conditions. The present invention does not have a special limitation on the stirring time, as long as it reaches the end point of the metathesis reaction. The present invention has no particular limitation on the stirring operation, and the stirring operation well known to those skilled in the art can be adopted. In the present invention, the end point of the metathesis reaction is preferably no carbon dioxide gas generation. The temperature of the metathesis reaction is not particularly limited in the present invention, and it can be under normal temperature conditions. In the present invention, the liquid obtained by metathesis reaction of Xiangye powder and choline can be used as a carrier of keratinocyte growth factor to increase the permeability of the skin.

After the ionic liquid is obtained, the present invention dries the ionic liquid to obtain an oily liquid. In the present invention, the drying temperature preferably does not exceed 70°C, more preferably 50-60°C. In the present invention, the drying time is preferably not less than 36h, more preferably 40-50h. The present invention has no special limitation on the drying operation, and the drying operation well known to those skilled in the art can be used. In the present invention, the drying is to remove the moisture in the ionic liquid.

In the present invention, the keratinocyte growth factor and the PBS buffer are mixed to obtain the growth factor solution. The present invention does not specifically limit the operation of mixing the keratinocyte growth factor and the PBS buffer, and the technical solution for preparing the mixed material well known to those skilled in the art can be used. In the present invention, the keratinocyte growth factor is mixed with the PBS buffer to dissolve the keratinocyte growth factor, while providing a relatively stable ionic environment to avoid the inactivation of the keratinocyte growth factor, thereby protecting the keratinocyte growth factor.

After the oily liquid and the growth factor solution are obtained, the present invention mixes the oily liquid and the growth factor solution to obtain a hair growth oil. The present invention has no particular limitation on the operation of mixing the oily liquid and the growth factor solution, and the two solutions may be mixed uniformly.

The preparation method provided by the invention is easy to operate, simple in process and low in cost, and is very suitable for rapid and large-scale production.

The present invention does not specifically limit the usage and dosage of the hair tonic, and the usage and dosage of liniment external medicines well known to those skilled in the art can be used.

The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some, but not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

Example 1

(1) get 48.195wt% geranic acid (purity of 90%) and 29.076wt% choline in a round-bottomed flask and stir at room temperature until no carbon dioxide gas is generated to obtain an ionic liquid;

(2) vacuum drying the ionic liquid obtained in step (1) at 60° C. for 48h to obtain an oily liquid;

(3) Dissolving 0.005wt% KGF-2 in 22.724wt% PBS buffer to obtain a growth factor solution;

(4) uniformly mixing the oily liquid obtained in the step (2) and the growth factor solution obtained in the step (3) to obtain a hair growth oil.

Example 2

(1) get 48.197wt% geranic acid (purity of 90%) and 29.077wt% choline in a round-bottomed flask and stir at room temperature until no carbon dioxide gas is generated to obtain an ionic liquid;

(2) vacuum drying the ionic liquid obtained in step (1) at 60° C. for 48h to obtain an oily liquid;

(3) Dissolving 0.001wt% KGF-2 in 22.725wt% PBS buffer to obtain a growth factor solution;

(4) uniformly mixing the oily liquid obtained in the step (2) and the growth factor solution obtained in the step (3) to obtain a hair growth oil.

Example 3

(1) get 48.192wt% geranic acid (purity is 90%) and 29.075wt% choline in a round bottom flask and stir at room temperature until no carbon dioxide gas is generated to obtain an ionic liquid;

(2) vacuum drying the ionic liquid obtained in step (1) at 60° C. for 48h to obtain an oily liquid;

(3) Dissolving 0.010wt% KGF-2 in 22.723wt% PBS buffer to obtain a growth factor solution;

(4) uniformly mixing the oily liquid obtained in the step (2) and the growth factor solution obtained in the step (3) to obtain a hair growth oil.

Comparative Example 1

(1) get 23.477wt% geranic acid (purity of 90%) and 28.815% choline in a round-bottomed flask and stir at room temperature until no carbon dioxide gas is generated to obtain an ionic liquid;

(2) vacuum drying the ionic liquid obtained in step (1) at 60° C. for 48h to obtain an oily liquid;

(3) Dissolving 0.005wt% KGF-2 in 47.703wt% PBS buffer to obtain a growth factor solution;

(4) uniformly mixing the oily liquid obtained in the step (2) and the growth factor solution obtained in the step (3) to obtain a hair growth oil.

The hair tonic oil prepared in Example 1 and control groups 1 to 3 (wherein control group 1 is a PBS buffer, control group 2 is a mixed solution of an ionic liquid composed of choline and geranic acid and a PBS buffer, and control group 3 is Growth factor solution, prepared by dissolving KGF-2 in PBS buffer, was used in animal experiments to investigate its effect.

Use a dropper to take 4 drops of the solutions of Example 1 and control groups 1 to 3, and apply them evenly on the skin of adult black mice whose back hair has been shaved. The results are shown in Figures 1 to 4. 1 is the comparison chart of the unsmeared control group 1 product, the control group 1 product applied for 7 days, 14 days and 21 days of black mice; Figure 2 is the unsmeared control group 2 product, applied with the control group 2 product for 7 days, 14 days and 21 days. The comparison chart of the black mice for 21 days; Figure 3 is the comparison chart of the black mice without applying the 3 products of the control group, and applying the 3 products of the control group for 7 days, 14 days and 21 days; Comparison diagram of black mice for 7 days, 14 days and 21 days of the product of Example 1.

As can be seen from Figures 1 to 4, within three weeks, compared with the control groups 1 to 3, the mice in Example 1 had the best hair growth effect on the shaved part, and most of the back hair could be restored to the length before being shaved. and density, it can be proved that the hair tonic oil of the present invention can improve the growth rate of hair.

The products prepared in Examples 1 to 3 and Comparative Example 1 were respectively applied to the dry scalps of 12 people, 1 mL of each was applied and massaged to promote drug absorption, twice a day, for two months. The length and density of the above hair were measured and the data are shown in Table 1.

Table 1 Measurement data of the products of Examples 1 to 3 and Comparative Example 1 acting on the scalp

As can be seen from Table 1, after applying the product for two months, Examples 1 to 3 of the present invention can better promote the regeneration and maturation of hair follicles compared to Comparative Example 1, indicating that the hair growth oil provided by the present invention is distributed in each group Excellent hair growth effect can be achieved within the ratio range.

It can be seen from the above examples that the hair tonic oil provided by the present invention has excellent hair growth effect.

The above are only the preferred embodiments of the present invention. It should be pointed out that for those skilled in the art, without departing from the principles of the present invention, several improvements and modifications can be made. It should be regarded as the protection scope of the present invention.

Claims (10)

1. A hair tonic, prepared from the raw materials of the following components: 45wt%-50wt% geranic acid, 25wt%-32wt% choline, 20wt%-25wt% PBS buffer and 0.001wt%-0.1wt% keratinocytes growth factor; The keratinocyte growth factor is KGF-2. 2. The hair tonic oil according to claim 1 is characterized in that, it is prepared from the raw materials of the following components: 48wt%-50wt% geranic acid, 29wt%-32wt% choline, 22wt%-25wt% PBS buffer and 0.001wt% to 0.05wt% keratinocyte growth factor. 3. The hair tonic oil according to claim 2, characterized in that, it is prepared from the raw materials of the following components: 48.192wt%～48.197wt% geranic acid, 29.075wt%～29.077wt% choline, 22.723wt%～ 22.725wt% PBS buffer and 0.001wt% to 0.005wt% keratinocyte growth factor. 4 . The hair tonic oil according to claim 1 , wherein the purity of the geranic acid is ≥85%. 5 . 5. the preparation method of the hair tonic of any one of claim 1～4, comprises the steps: (1) geranic acid and choline are mixed to carry out metathesis reaction to obtain ionic liquid; (2) drying the ionic liquid obtained in the step (1) to obtain an oily liquid; (3) mixing keratinocyte growth factor and PBS buffer to obtain growth factor solution; (4) mixing the oily liquid obtained in the step (2) with the growth factor solution obtained in the step (3) to obtain hair growth oil; The steps (1)-(2) and step (3) are not in order. 6 . The preparation method according to claim 5 , wherein the mixing in the step (1) is carried out under stirring conditions. 7 . 7 . The preparation method according to claim 5 , wherein the end point of the metathesis reaction in the step (1) is no carbon dioxide gas generation. 8 . 8 . The preparation method according to claim 5 , wherein the drying temperature in the step (2) does not exceed 70° C. 9 . 9 . The preparation method according to claim 8 , wherein the drying temperature in the step (2) is 50-60° C. 10 . 10. The preparation method according to claim 5, wherein the drying time in the step (2) is not less than 36h.

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