CN111849818A - Biological aerobic fermentation bacteria - Google Patents
Biological aerobic fermentation bacteria Download PDFInfo
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- CN111849818A CN111849818A CN202010732321.6A CN202010732321A CN111849818A CN 111849818 A CN111849818 A CN 111849818A CN 202010732321 A CN202010732321 A CN 202010732321A CN 111849818 A CN111849818 A CN 111849818A
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- 238000010564 aerobic fermentation Methods 0.000 title claims abstract description 18
- 241000894006 Bacteria Species 0.000 title claims description 9
- 240000006439 Aspergillus oryzae Species 0.000 claims abstract description 38
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims abstract description 38
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 38
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 38
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 38
- 241000186361 Actinobacteria <class> Species 0.000 claims abstract description 37
- 244000168141 Geotrichum candidum Species 0.000 claims abstract description 37
- 235000017388 Geotrichum candidum Nutrition 0.000 claims abstract description 37
- 238000001035 drying Methods 0.000 claims abstract description 30
- 238000000855 fermentation Methods 0.000 claims abstract description 30
- 230000004151 fermentation Effects 0.000 claims abstract description 28
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 11
- 238000012258 culturing Methods 0.000 claims abstract description 10
- 238000002156 mixing Methods 0.000 claims abstract description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 21
- 238000001291 vacuum drying Methods 0.000 claims description 13
- 239000010902 straw Substances 0.000 claims description 7
- 239000000126 substance Substances 0.000 abstract description 7
- 239000000758 substrate Substances 0.000 abstract description 5
- 238000000354 decomposition reaction Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 210000003608 fece Anatomy 0.000 description 6
- 239000010871 livestock manure Substances 0.000 description 6
- 102000010911 Enzyme Precursors Human genes 0.000 description 3
- 108010062466 Enzyme Precursors Proteins 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 244000144977 poultry Species 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 239000010806 kitchen waste Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002068 microbial inoculum Substances 0.000 description 2
- 239000010815 organic waste Substances 0.000 description 2
- 239000002910 solid waste Substances 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- Organic Chemistry (AREA)
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- General Engineering & Computer Science (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract
The invention discloses a biological aerobic zymocyte, belonging to the technical field of biological fermentation, which is prepared by respectively culturing, fermenting, drying and mixing bacillus licheniformis, bacillus subtilis, saccharomycetes, actinomycetes, aspergillus oryzae and geotrichum candidum. The biological aerobic zymophyte can efficiently carry out aerobic and anaerobic fermentation under different conditions, obviously improves the fermentation decomposition efficiency and effect of the substrate, and realizes the full reuse of the substances.
Description
Technical Field
The invention belongs to the technical field of biological fermentation, and particularly relates to a biological aerobic fermentation bacterium.
Background
The rapid development of the large-scale and intensive livestock and poultry breeding industry makes great contribution to the improvement of the living level of people, and simultaneously generates a large amount of livestock and poultry manure to cause serious environmental pollution. However, only about 30% of organic wastes of the current intensive farms are primarily treated and utilized, and 70% of the organic wastes are directly discharged to the environment. Solving the problems of the discharge of livestock and poultry manure and environmental pollution becomes a big problem related to the survival and development of human beings and the development of ecological agriculture in the 21 st century. The animal manure, especially the cattle (sheep) manure, contains high organic matter content and also contains a certain amount of nitrogen, phosphorus, potassium, calcium, magnesium, various trace elements and other nutrient components. Can be used as raw material of organic fertilizer.
In addition, in other economic development production or life, solid wastes such as factory solid wastes, kitchen wastes, production wastes and the like are generated, and the substances also have high utilization value, but are mostly treated by landfill, so that the material resources are wasted.
The biological aerobic zymophyte is a microbial inoculum mainly used for aerobic fermentation, can perform good fermentation decomposition treatment on the substances, promotes the substances to be recycled, and improves the utilization rate of the substances. However, the existing biological aerobic zymogens mostly have the problems of low fermentation speed, complex fermentation operation and high cost.
Disclosure of Invention
The invention aims to provide a biological aerobic zymocyte.
The technical purpose of the invention is realized by the following technical scheme:
a biological aerobic zymocyte is prepared from Bacillus licheniformis, Bacillus subtilis, yeast, actinomycetes, Aspergillus oryzae, and Geotrichum candidum by culturing, fermenting, drying, and mixing; the weight portions are as follows:
15-25 parts of bacillus licheniformis, 10-15 parts of bacillus subtilis, 8-10 parts of saccharomycetes, 20-25 parts of actinomycetes, 14-18 parts of aspergillus oryzae and 6-10 parts of geotrichum candidum.
Preferably, the weight portions are specifically as follows:
15 parts of bacillus licheniformis, 10 parts of bacillus subtilis, 8 parts of yeast, 20 parts of actinomycetes, 14 parts of aspergillus oryzae and 6 parts of geotrichum candidum.
Preferably, the weight portions are specifically as follows:
20 parts of bacillus licheniformis, 13 parts of bacillus subtilis, 9 parts of yeast, 22 parts of actinomycetes, 16 parts of aspergillus oryzae and 8 parts of geotrichum candidum.
Preferably, the weight portions are specifically as follows:
25 parts of bacillus licheniformis, 15 parts of bacillus subtilis, 10 parts of yeast, 25 parts of actinomycetes, 18 parts of aspergillus oryzae and 10 parts of geotrichum candidum.
Furthermore, the effective viable count of the bacillus licheniformis, the bacillus subtilis, the microzyme, the actinomycetes, the aspergillus oryzae and the geotrichum candidum is 5 multiplied by 1010More than one/ml.
Further, the culture treatment is carried out for 20-28 h under the conditions that the temperature is 26-32 ℃ and the rpm is 180-190 rpm.
Further, the fermentation treatment is to mix and ferment culture solution of bacillus licheniformis, bacillus subtilis, saccharomycetes, actinomycetes, aspergillus oryzae and geotrichum candidum and fermented bran or fermented straw according to the weight ratio of 1:1, and the fermentation time is controlled to be 38-46 h.
Furthermore, the drying treatment adopts a vacuum drying treatment mode.
Further, the drying temperature is controlled to be 60-65 ℃ during vacuum drying treatment, and the vacuum degree is 15-20 Pa during drying.
Compared with the prior art, the invention has the following advantages:
the invention discloses a biological aerobic zymocyte which is prepared by respectively culturing, fermenting, drying and mixing bacillus licheniformis, bacillus subtilis, saccharomycetes, actinomycetes, aspergillus oryzae and geotrichum candidum, reasonably collocates a plurality of strains, adjusts the optimal proportion, ensures that the aerobic and anaerobic fermentation can be efficiently carried out under different conditions, obviously improves the fermentation and decomposition efficiency and effect of a substrate, realizes the full reutilization of substances, and has strong use and popularization values and market competitiveness.
Detailed Description
Example 1
A biological aerobic zymocyte is prepared from Bacillus licheniformis, Bacillus subtilis, yeast, actinomycetes, Aspergillus oryzae, and Geotrichum candidum by culturing, fermenting, drying, and mixing; the weight portions are as follows:
15 parts of bacillus licheniformis, 10 parts of bacillus subtilis, 8 parts of yeast, 20 parts of actinomycetes, 14 parts of aspergillus oryzae and 6 parts of geotrichum candidum.
The effective viable count of the bacillus licheniformis, the bacillus subtilis, the microzyme, the actinomycetes, the aspergillus oryzae and the geotrichum candidum is 5 multiplied by 1010More than one/ml.
The culture treatment is carried out at 26 deg.C and 180rpm for 20 h.
The fermentation treatment is to mix and ferment culture solution of bacillus licheniformis, bacillus subtilis, saccharomycetes, actinomycetes, aspergillus oryzae and geotrichum candidum and fermented bran or fermented straw correspondingly according to the weight ratio of 1:1, and the fermentation time is controlled to be 38 hours.
The drying treatment adopts a vacuum drying treatment mode.
The drying temperature is controlled to be 60 ℃ during the vacuum drying treatment, and the vacuum degree is controlled to be 15Pa during the drying.
Example 2
A biological aerobic zymocyte is prepared from Bacillus licheniformis, Bacillus subtilis, yeast, actinomycetes, Aspergillus oryzae, and Geotrichum candidum by culturing, fermenting, drying, and mixing; the weight portions are as follows:
20 parts of bacillus licheniformis, 13 parts of bacillus subtilis, 9 parts of yeast, 22 parts of actinomycetes, 16 parts of aspergillus oryzae and 8 parts of geotrichum candidum.
The effective viable count of the bacillus licheniformis, the bacillus subtilis, the microzyme, the actinomycetes, the aspergillus oryzae and the geotrichum candidum is 5 multiplied by 1010More than one/ml.
The culture treatment is carried out at 30 ℃ and 185rpm for 26 h.
The fermentation treatment is to mix and ferment culture solution of bacillus licheniformis, bacillus subtilis, saccharomycetes, actinomycetes, aspergillus oryzae and geotrichum candidum and fermented bran or fermented straw correspondingly according to the weight ratio of 1:1, and the fermentation time is controlled to be 42 hours.
The drying treatment adopts a vacuum drying treatment mode.
The drying temperature during the vacuum drying treatment is controlled to be 63 ℃, and the vacuum degree during the drying is 18 Pa.
Example 3
A biological aerobic zymocyte is prepared from Bacillus licheniformis, Bacillus subtilis, yeast, actinomycetes, Aspergillus oryzae, and Geotrichum candidum by culturing, fermenting, drying, and mixing; the weight portions are as follows:
25 parts of bacillus licheniformis, 15 parts of bacillus subtilis, 10 parts of yeast, 25 parts of actinomycetes, 18 parts of aspergillus oryzae and 10 parts of geotrichum candidum.
The effective viable count of the bacillus licheniformis, the bacillus subtilis, the microzyme, the actinomycetes, the aspergillus oryzae and the geotrichum candidum is 5 multiplied by 1010More than one/ml.
The culture treatment is carried out for 28h under the conditions of temperature of 32 ℃ and 190 rpm.
The fermentation treatment is to mix and ferment culture solution of bacillus licheniformis, bacillus subtilis, saccharomycetes, actinomycetes, aspergillus oryzae and geotrichum candidum and fermented bran or fermented straw correspondingly according to the weight ratio of 1:1, and the fermentation time is controlled to be 46 h.
The drying treatment adopts a vacuum drying treatment mode.
The drying temperature is controlled to be 65 ℃ during the vacuum drying treatment, and the vacuum degree is controlled to be 20Pa during the drying.
Example 4
A biological aerobic zymocyte is prepared from Bacillus licheniformis, Bacillus subtilis, yeast, actinomycetes, Aspergillus oryzae, and Geotrichum candidum by culturing, fermenting, drying, and mixing; the weight portions are as follows:
15 parts of bacillus licheniformis, 13 parts of bacillus subtilis, 9 parts of yeast, 22 parts of actinomycetes, 16 parts of aspergillus oryzae and 6 parts of geotrichum candidum.
The bacillus licheniformis, the bacillus subtilis, the microzyme and the actinomycetesThe effective viable count of the bacteria, Aspergillus oryzae and Geotrichum candidum is 5 × 1010More than one/ml.
The culture treatment is carried out at the temperature of 26 ℃ and the speed of 185rpm for 24 h.
The fermentation treatment is to mix and ferment culture solution of bacillus licheniformis, bacillus subtilis, saccharomycetes, actinomycetes, aspergillus oryzae and geotrichum candidum and fermented bran or fermented straw correspondingly according to the weight ratio of 1:1, and the fermentation time is controlled to be 40 h.
The drying treatment adopts a vacuum drying treatment mode.
The drying temperature is controlled to be 605 ℃ during the vacuum drying treatment, and the vacuum degree is controlled to be 15Pa during the drying.
Example 5
A biological aerobic zymocyte is prepared from Bacillus licheniformis, Bacillus subtilis, yeast, actinomycetes, Aspergillus oryzae, and Geotrichum candidum by culturing, fermenting, drying, and mixing; the weight portions are as follows:
20 parts of bacillus licheniformis, 13 parts of bacillus subtilis, 9 parts of yeast, 25 parts of actinomycetes, 18 parts of aspergillus oryzae and 10 parts of geotrichum candidum.
The effective viable count of the bacillus licheniformis, the bacillus subtilis, the microzyme, the actinomycetes, the aspergillus oryzae and the geotrichum candidum is 5 multiplied by 1010More than one/ml.
The culture treatment is carried out for 28h at the temperature of 28 ℃ and the speed of 190 rpm.
The fermentation treatment is to mix and ferment culture solution of bacillus licheniformis, bacillus subtilis, saccharomycetes, actinomycetes, aspergillus oryzae and geotrichum candidum and fermented bran or fermented straw correspondingly according to the weight ratio of 1:1, and the fermentation time is controlled to be 44 hours.
The drying treatment adopts a vacuum drying treatment mode.
The drying temperature is controlled to be 65 ℃ during the vacuum drying treatment, and the vacuum degree is controlled to be 20Pa during the drying.
Control group 1
A biological aerobic fermentation bacteria is prepared by respectively culturing Bacillus licheniformis, Bacillus subtilis, yeast, actinomycetes and Aspergillus oryzae, fermenting, drying and mixing to obtain the biological aerobic fermentation bacteria; the weight portions are as follows:
20 parts of bacillus licheniformis, 13 parts of bacillus subtilis, 9 parts of yeast, 22 parts of actinomycetes and 16 parts of aspergillus oryzae.
The comparison of control 1 with example 2 only differs from the comparison in that the geotrichum candidum component is omitted, and the steps are the same except for this.
In order to compare the effects of the invention, fermentation experiments are carried out on the zymogens prepared correspondingly in the above examples 1-5 and the control group 1 and a commercially available aerobic fermentation microbial inoculum, the substrates are respectively pig manure, chicken manure and kitchen waste, then the corresponding zymogens are inoculated and then are subjected to conventional fermentation, the management and control modes of the substrates, the environmental conditions and the like during the fermentation are completely the same, then the time duration required for the central temperature of each substrate to reach 65 ℃ is counted, and the specific comparison data are shown in the following table 1:
TABLE 1
As can be seen from the above table 1, the aerobic fermentation tubes of the invention can rapidly realize the fermentation and decomposition of substances, rapidly increase the temperature of materials, promote the acceleration of the process and have good popularization and application values.
Claims (9)
1. A biological aerobic zymocyte is characterized in that the biological aerobic zymocyte is prepared by respectively culturing, fermenting, drying and mixing bacillus licheniformis, bacillus subtilis, saccharomycetes, actinomycetes, aspergillus oryzae and geotrichum candidum; the weight portions are as follows:
15-25 parts of bacillus licheniformis, 10-15 parts of bacillus subtilis, 8-10 parts of saccharomycetes, 20-25 parts of actinomycetes, 14-18 parts of aspergillus oryzae and 6-10 parts of geotrichum candidum.
2. The biological aerobic fermentation bacteria of claim 1, which are specifically calculated by the weight parts as follows:
15 parts of bacillus licheniformis, 10 parts of bacillus subtilis, 8 parts of yeast, 20 parts of actinomycetes, 14 parts of aspergillus oryzae and 6 parts of geotrichum candidum.
3. The biological aerobic fermentation bacteria of claim 1, which are specifically calculated by the weight parts as follows:
20 parts of bacillus licheniformis, 13 parts of bacillus subtilis, 9 parts of yeast, 22 parts of actinomycetes, 16 parts of aspergillus oryzae and 8 parts of geotrichum candidum.
4. The biological aerobic fermentation bacteria of claim 1, which are specifically calculated by the weight parts as follows:
25 parts of bacillus licheniformis, 15 parts of bacillus subtilis, 10 parts of yeast, 25 parts of actinomycetes, 18 parts of aspergillus oryzae and 10 parts of geotrichum candidum.
5. The biological aerobic fermentation strain of any one of claims 1 to 4, wherein the effective viable count of the Bacillus licheniformis, the Bacillus subtilis, the yeast, the actinomycetes, the Aspergillus oryzae and the Geotrichum candidum is 5 x 1010More than one/ml.
6. A biological aerobic fermentation broth according to any of claims 1 to 4 wherein the cultivation is carried out at a temperature of 26 to 32 ℃ and 180 to 190rpm for 20 to 28 hours.
7. The biological aerobic fermentation strain according to any one of claims 1 to 4, wherein the fermentation treatment is a mixed fermentation of a culture solution of Bacillus licheniformis, Bacillus subtilis, yeast, actinomycetes, Aspergillus oryzae and Geotrichum candidum and fermentation bran or fermentation straw in a weight ratio of 1:1, and the fermentation time is controlled to be 38 to 46 hours.
8. A biological aerobic fermentation tube according to any of claims 1 to 4, wherein the drying treatment is a vacuum drying treatment.
9. A biological aerobic fermentation broth according to claim 8 wherein the temperature of drying is controlled to 60-65 ℃ and the vacuum degree is controlled to 15-20 Pa.
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| CN202010732321.6A CN111849818A (en) | 2020-07-27 | 2020-07-27 | Biological aerobic fermentation bacteria |
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| CN202010732321.6A CN111849818A (en) | 2020-07-27 | 2020-07-27 | Biological aerobic fermentation bacteria |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113115714A (en) * | 2021-04-14 | 2021-07-16 | 甘肃省畜牧兽医研究所 | Method for recycling wastes of large-scale farm |
Citations (3)
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|---|---|---|---|---|
| CN102531766A (en) * | 2011-09-15 | 2012-07-04 | 北京世纪阿姆斯生物技术股份有限公司 | Microbial decomposing agent and production method thereof |
| CN103667117A (en) * | 2013-11-18 | 2014-03-26 | 广西金穗生物科技有限责任公司 | Compound microbial bacterium for aerobic fermentation stage |
| CN109423462A (en) * | 2017-09-04 | 2019-03-05 | 上海康地源生物科技有限公司 | One kind being used for straw and the decomposed complex microorganism preparations manufacturing method of animal wastes |
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- 2020-07-27 CN CN202010732321.6A patent/CN111849818A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102531766A (en) * | 2011-09-15 | 2012-07-04 | 北京世纪阿姆斯生物技术股份有限公司 | Microbial decomposing agent and production method thereof |
| CN103667117A (en) * | 2013-11-18 | 2014-03-26 | 广西金穗生物科技有限责任公司 | Compound microbial bacterium for aerobic fermentation stage |
| CN109423462A (en) * | 2017-09-04 | 2019-03-05 | 上海康地源生物科技有限公司 | One kind being used for straw and the decomposed complex microorganism preparations manufacturing method of animal wastes |
Non-Patent Citations (1)
| Title |
|---|
| 韩梦颖 等: "降解秸秆微生物及秸秆腐熟剂的研究进展", 《南方农业学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113115714A (en) * | 2021-04-14 | 2021-07-16 | 甘肃省畜牧兽医研究所 | Method for recycling wastes of large-scale farm |
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