CN111812290B - Water pollution monitoring biosensor, monitoring system and monitoring method - Google Patents

Abstract

The invention discloses a water pollution monitoring biosensor, a monitoring system and a monitoring method, wherein the monitoring biosensor comprises the following components: aquatic organisms, which are living bivalve organisms; the grating fixing structure comprises a first fixing base, a second fixing base and an elastic beam piece, wherein the elastic beam piece is of an arch structure, and a first fixing part and a second fixing part of the elastic beam piece are respectively arranged on the first fixing base and the second fixing base, so that a bending part in the middle of the elastic beam piece is positioned between the two fixing bases; the two fixed bases are respectively fixed on the surfaces of the two shells of the aquatic organisms; the grating is a fiber Bragg grating and is adhered to the outer side of the bending part of the grating fixing structure; the opening and closing of the two shells of the aquatic organisms drive the bending parts to expand or contract through the two fixing bases, and then the grating is driven to regularly expand or contract to generate signal change.

Description

Water pollution monitoring biosensor, monitoring system and monitoring method

technical field

The invention relates to the technical field of water pollution monitoring, in particular to a biosensor for water pollution monitoring, a monitoring system and a monitoring method.

Background technique

The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art.

Conventional water quality monitoring often requires local sampling at the water source, and the sample water is brought back to the laboratory or testing center for offline testing. This technical method has a long monitoring cycle, poor timeliness, poor data reliability, and high cost consumption. Online monitoring is not possible.

At present, there are some electronic products that can realize online water quality monitoring, but such electronic products need power supply, and it is difficult to deploy them at multiple points in large-scale waters, especially in seawater, where they are easy to be attached by aquatic organisms and become invalid. Long-term work in marine corrosive environment. It is a new technology to install sensors on organisms to realize environmental monitoring, but most of them are realized by electronic sensors, which also have problems such as corrosion resistance, difficulty in power supply, and susceptibility to signal transmission interference, which cannot be monitored online for a long time. Conducive to the monitoring of water pollution.

Contents of the invention

In order to solve the technical problems existing in the prior art, the object of the present invention is to provide a water pollution monitoring biosensor, a monitoring system and a monitoring method.

In order to solve the above technical problems, one or more embodiments of the present invention provide the following technical solutions:

In a first aspect, the present invention provides a water pollution monitoring biosensor, comprising:

Aquatic organisms are living bivalve organisms;

The grating fixing structure includes a first fixing base, a second fixing base and an elastic beam. The elastic beam is an arched structure, and its first fixing part and second fixing part are respectively installed on the first fixing base and the second fixing base. On the fixed base, the curved part in the middle is located between the two fixed bases; the two fixed bases are respectively fixed on the two shell surfaces of the aquatic organisms;

The grating, which is a fiber Bragg grating, is adhered to the outside of the curved portion of the grating fixing structure;

The opening and closing of the two shells of the aquatic organism drives the expansion or contraction of the curved part through the two fixed bases, and then drives the regular expansion or contraction of the grating, resulting in a signal change.

In a second aspect, the present invention provides a water pollution monitoring system, comprising:

The water pollution monitoring biosensor;

The demodulator is connected with the grating of the biosensor through an optical cable;

The computer is connected with the demodulator.

In a third aspect, the present invention provides a water pollution monitoring method, comprising the steps of:

The respiration of the bivalve mollusk organisms causes the opening and closing movement of the two shells, which is transmitted to the elastic beam through the two fixed bases, causing the expansion or compression of the curved part of the elastic beam, and then bending The grating on the upper part is deformed synchronously, causing the central wavelength of the grating to change;

By monitoring the change rule of the central wavelength of the grating, the water quality of the water area where the bivalve mollusk organisms are located is monitored.

Compared with the prior art, the above one or more technical solutions of the present invention have achieved the following beneficial effects:

When the water pollution monitoring biosensor is in use, fix the first fixed base and the second fixed base on bivalve mollusks such as scallops, and then place them in the water body to be monitored. Changes, real-time response to a series of indicators such as water pollution, oxygen content, and pesticide content to complete the monitoring of water pollution, realize the monitoring of water pollution with the help of biological carriers, and facilitate water body monitoring.

The fiber Bragg grating is attached to the outside of the bending part, which is more prone to uniform strain and then generates regular signals. In addition, the bending part has a certain degree of elasticity, can be reset in time, and will not appear plastic deformation within a certain force range, so as to ensure the accuracy of monitoring.

The water pollution monitoring method is to use bivalve mollusc organisms as the sensing body of water pollution degree, and obtain the activity characteristics of organisms through optical fiber sensor collection, thereby realizing the monitoring of water pollution indicators. For water pollution monitoring, especially for organisms Water pollution monitoring that can have a direct impact has a more effective and direct on-line monitoring effect.

Fiber Bragg gratings are used to sense the activity characteristics of bivalve molluscs, and the strain signals of the gratings are transmitted outwards through optical cables. When monitoring in this way, the monitoring distance can reach hundreds of kilometers without relay stations or power supply, which solves the problem At present, the difficulty of off-line sampling of biosensors and remote long-term online monitoring of electronic sensors provides necessary monitoring means for the laying of marine information monitoring networks.

In addition, multiple biosensors can be connected in series, and the series detection of multiple sensors can be realized by using wavelength division multiplexing, which greatly reduces the difficulty of wiring and branching, and provides convenience for actual ocean layout.

Description of drawings

The accompanying drawings constituting a part of the present invention are used to provide a further understanding of the present invention, and the schematic embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations to the present invention.

Fig. 1 is the structural representation of the water pollution monitoring biosensor provided by the embodiment of the present invention;

Fig. 2 is a partial structural schematic diagram of a water pollution monitoring biosensor provided by an embodiment of the present invention;

Fig. 3 is a schematic structural diagram of an elastic beam provided by an embodiment of the present invention;

Fig. 4 is a schematic structural diagram of a first fixed base provided by an embodiment of the present invention;

Fig. 5 is a schematic structural diagram of a second fixed base provided by an embodiment of the present invention;

FIG. 6 is a schematic structural diagram of a water pollution monitoring system provided by an embodiment of the present invention;

Fig. 7 is an example diagram of healthy and sick waveforms of bivalve organisms provided by the embodiment of the present invention;

Fig. 8 is a waveform diagram of respiration, filter feeding and dormancy of bivalve mollusc organisms provided by an embodiment of the present invention in a normal water quality environment.

Wherein, 1, the first fixed base, 101, the first card slot, 102, the first hole;

2. The second fixed base, 201, the second card slot, 202, the second hole, 203, the fiber slot;

3. The elastic beam, 301, the first fixing part, 302, the second fixing part, 303, the bending part;

4. Optical fiber Bragg grating, 5. Isolation chamber, 6. Optical cable, 7. Optical cable buffer protection sleeve, 8. Computer, 9. Demodulator, 10. Bivalve mollusk.

Detailed ways

It should be noted that the following detailed description is exemplary and intended to provide further explanation of the present invention. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

It should be noted that the terminology used here is only for describing specific embodiments, and is not intended to limit exemplary embodiments according to the present invention. As used herein, unless the context clearly dictates otherwise, the singular is intended to include the plural, and it should also be understood that when the terms "comprising" and/or "comprising" are used in this specification, they mean There are features, steps, operations, means, components and/or combinations thereof.

In a first aspect, the present invention provides a water pollution monitoring biosensor, comprising:

Aquatic organisms are living bivalve organisms;

The grating fixing structure includes a first fixing base, a second fixing base and an elastic beam. The elastic beam is an arched structure, and its first fixing part and second fixing part are respectively installed on the first fixing base and the second fixing base. On the fixed base, the curved part in the middle is located between the two fixed bases; the two fixed bases are respectively fixed on the two shell surfaces of the aquatic organisms;

The grating, which is a fiber Bragg grating, is adhered to the outside of the curved portion of the grating fixing structure;

The opening and closing of the two shells of the aquatic organism drives the expansion or contraction of the curved part through the two fixed bases, and then drives the regular expansion or contraction of the grating, resulting in a signal change.

In some embodiments, when the bending portion is not elastically deformed, the first fixing base is parallel to the second fixing base. With this structure, the opening and closing between the two fixed bases can be transmitted to the bending part more sensitively, thereby making the deformation of the bending part more sensitive, which is beneficial to improving the sensitivity of monitoring.

In some embodiments, the outer sidewall of the curved portion is in the isolation chamber, and the interior of the isolation chamber is filled with isolation liquid.

The outer wall of the curved part refers to the side wall of the convex side of the curved part, the grating is pasted on the side wall, and the grating is placed in the spacer fluid, which can isolate the grating from the outside water body to ensure the normal operation of the grating , and guarantee the service life of the grating.

Further, the material of the waterproof partition wall is rubber or plastic.

Further, the spacer fluid is silicone oil. Silicone oil has high heat resistance, water resistance, electrical insulation and low surface tension, and can be used as a release agent. Other liquid components are also available, and must have the characteristics of no corrosion to metal and rubber, and the characteristics of non-freezing, non-vaporization, and small expansion coefficient in the four-season temperature range of conventional seawater or freshwater.

Further, the isolation bin is wrapped on the outside of the elastic beams and the grating.

Furthermore, one end of the isolation bin is fixed on the first fixed base, and the other end is fixed on the second fixed base.

In some embodiments, the first fixing base is provided with a first card slot, the second fixing base is provided with a second card slot, and the first fixing part and the second fixing part of the elastic beam are inserted into the first fixing part respectively. Inside the first card slot and the second card slot.

Further, the elastic beam is made of stainless steel or fiber composite material. It has strong elasticity and resistance to seawater erosion.

In some embodiments, the first fixing base and the second fixing base are bonded to the shell of the bivalve organism by waterproof glue.

Further, a first hole is provided at the bonding position of the first fixed base and the housing, and a second hole is provided at the bonding position of the second fixed base and the housing, the first hole and the second hole are both through hole.

The arrangement of the first hole and the second hole is convenient to apply waterproof glue between the fixed base and the shell, so as to improve the bonding strength between the fixed base and the shell. In addition, with this structure, the fixed base and the housing can be positioned first, and then the adhesive can be injected inward through the first hole and the second hole, which helps to better realize the positioning and fixing of the fixed base.

In some embodiments, the first fixed base or the second fixed base is provided with a groove.

The groove is used to place the optical cable so as to connect the optical cable with the grating.

In a second aspect, the present invention provides a water pollution monitoring system, comprising:

The water pollution monitoring biosensor;

The demodulator is connected with the grating of the biosensor through an optical cable;

The computer is connected with the demodulator.

In some embodiments, an optical cable buffer and protective sheath is provided at the connection between the grating and the optical cable. It is used to protect the connection between fiber Bragg grating and optical cable.

Further, the optical cable buffer protection sleeve is arranged in the optical fiber groove.

The number of biosensors for water pollution monitoring can be expanded according to actual needs, and the sensors are connected in series.

In a third aspect, the present invention provides a water pollution monitoring method, comprising the steps of:

The respiration of the bivalve mollusk organisms causes the opening and closing movement of the two shells, which is transmitted to the elastic beam through the two fixed bases, causing the expansion or compression of the curved part of the elastic beam, and then bending The grating on the upper part is deformed synchronously, causing the central wavelength of the grating to change;

By monitoring the change rule of the central wavelength of the grating, the water quality of the water area where the bivalve mollusk organisms are located is monitored.

An embodiment of the present invention provides a novel biosensor for water pollution monitoring. The water pollution monitoring sensor is used to be fixed on bivalve mollusks such as scallops. Fig. 1 is a schematic structural diagram of a water pollution monitoring sensor provided by an embodiment of the present invention. As shown in FIG. 1 , the water pollution monitoring sensor provided by the embodiment of the present invention includes a first fixed base 1 , a second fixed base 2 , an elastic beam 3 and a fiber Bragg grating 4 .

One end of the elastic beam 3 is fixedly connected to the first fixed base 1, and the other end of the elastic beam 3 is fixedly connected to the second fixed base 2, that is, the elastic beam 3 realizes connecting the first fixed base 1 and the second fixed base. 2 connected together. The first fixed base 1 and the second fixed base 2 are used for fixedly connecting shells of bivalve molluscs such as scallops. The elastic beam 3 has good elasticity and can play a good transmission role. In the embodiment of the present invention, the rear ends of the first fixed base 1 and the second fixed base 2 are connected to the elastic beam 3, and the front ends of the first fixed base 1 and the second fixed base 2 are used for fixedly connecting scallops, etc. bivalve mollusk organisms.

When the water pollution monitoring sensor is fixed on the organism through the first fixed base 1 and the second fixed base 2, the breathing of the organism causes the opening and closing of the upper and lower shells, thereby driving the first fixed base 1 and the second fixed base 2. The opening and closing movement of the second fixed base 2 , and the opening and closing movements of the first fixed base 1 and the second fixed base 2 are transmitted to the elastic beam 3 , causing bending deformation of the elastic beam 3 . The fiber Bragg grating 4 is fixedly arranged on the elastic beam 3. When the elastic beam 3 is bent and deformed, the bending deformation will cause the fiber Bragg grating 4 close to the elastic beam 3 to simultaneously generate strain, thereby causing the fiber Bragg Change of grating 4 center wavelength.

The central reflection wavelength of the fiber Bragg grating is:

(1), where: is the effective refractive index of the guided mode, is the natural period of the grating.

When the wavelength satisfies the Bragg condition (1), the incident light will be reflected back by the fiber grating.

From the formula (1), it can be known that the central reflection wavelength of the fiber grating with and changed by changes. Fiber Bragg gratings are sensitive to stress, and the stress passes through the elasto-optic effect and the period of the fiber grating changes to affect .

When the fiber grating is only subjected to stress, the refractive index and period of the fiber grating change, causing the central reflection wavelength move, so there are:

(2)

In the formula: is the change in the refractive index, is the change of grating period.

Refractive index change when the grating is stressed:

(3)

In the formula:

(4)

is the axial stress, is the Poisson's ratio of the core material, , is the elastic-optical coefficient, is the effective elastic-optical coefficient.

Assume that the fiber grating is absolutely uniform, that is, the relative rate of change of the period of the grating is consistent with the relative rate of change of the physical length of the grating segment.

(5)

So formula (3) can be written as:

(6)

Formula (6) is the general calculation formula for bare fiber grating stress measurement.

axial stress It is caused by the bending of the beam caused by the opening and closing of the bivalve organisms that the grating is subjected to, that is, the opening and closing of the bivalve organisms causes the stress change of the fiber grating, and the bivalve respiration can be established by formula (6). And the opening and closing degree Q of the filter-feeding shell and the central wavelength of the fiber grating Corresponding relationship:

Δ (7), where, is the slope coefficient.

According to the above formula, as long as the variation of the center wavelength of the grating is measured, the frequency characteristics of opening and closing respiration and filter feeding of bivalve organisms can be measured.

In the embodiment of the present invention, bivalve molluscs are preferably scallops, which have high viability, but are not limited to scallops, and can be selected according to the actual detection of water bodies. When selecting scallops for use, as shown in accompanying drawing 1, bivalve mollusk 10 is clamped on the first fixed base 1 and the second fixed base 2 one end away from its fixedly connected elastic beam sheet 3, the first fixed base The seat 1 and the second fixed base 2 are respectively fixedly connected to the upper and lower shells of the bivalve mollusk 10 .

The corresponding relationship between the respiration and feeding laws of organisms and water quality components can establish a professional database according to the changes in different components and biological characteristics. Different types of shells have different responses to different components, and the same type of shells respond to different components. The relationship is also different, but the corresponding response relationship between certain shells and certain components is certain. For example, there is an obvious corresponding relationship between oil pollution and scallop opening and closing respiratory activity. Therefore, the monitoring of water pollution is completed by monitoring the changes in the physiological characteristics of organisms in real time to reflect a series of indicators such as water pollution, oxygen content, and pesticide content, and realizes the monitoring of water pollution with the help of biological carriers, which is convenient for water body monitoring.

The water pollution monitoring sensor provided by the embodiments of the present invention is mainly used in water body environment, such as ocean water body and the like. In order to prevent the water body from corroding the elastic beam 3 and the fiber Bragg grating 4 in the water body or being attached by water body organisms to affect the use of the water pollution monitoring sensor, the water pollution monitoring sensor provided in the embodiment of the present invention also includes an isolation chamber 5 . The isolation bin 5 is fixedly connected to the first fixed base 1 and the second fixed base 2, and the elastic beam 3 and the fiber Bragg grating 4 are located in the isolation bin 5, and the isolation bin 5 is used to prevent the elastic beam 3 and the fiber Bragg grating 4 from Corroded in water bodies or attached by water body organisms. The isolation bin 5 is made of elastic soft material. Optionally, the elastic soft material of the isolation chamber 5 can be selected from waterproof rubber material or waterproof film material. Further, the isolation chamber 5 is filled with silicone oil, which can better protect the elastic beam 3 and the fiber Bragg grating 4, and facilitate the use of the water pollution monitoring sensor underwater.

The elastic beam 3 can be made of high Cr, Ni and low C seawater corrosion-resistant stainless steel, FeAlMo alloy stainless steel, elastic seawater corrosion-resistant fiber composite material, etc., so that the elastic beam 3 has strong elasticity and seawater corrosion resistance.

The isolation bin 5 includes an isolation bin inner sheet and an isolation bin outer sheet, one end of the isolation bin inner sheet and the isolation bin outer sheet is fixedly connected to the first fixed base 1, and the other end is fixedly connected to the second fixed base 2, and the isolation bin outer sheet is located at The end of the first fixed base 1 and the second fixed base 2, the inner side of the isolation compartment is located on the opposite inner side of the first fixed base 1 and the second fixed base 2, and the inner side of the isolation compartment is connected to the outer side of the isolation compartment form a cavity, and wrap the elastic beam 3 and the fiber Bragg grating 4 inside.

Fig. 2 is a schematic diagram of a partial structure of a water pollution monitoring sensor provided by an embodiment of the present invention. Fig. 2 shows the basic structure of the elastic beam 3, the first fixed base 1 and the second fixed base 2 in the embodiment of the present invention. Figure 3 is a schematic structural view of the elastic beam provided in the embodiment of the present invention; Figure 4 is a schematic structural view of the first fixed base provided in the embodiment of the present invention; Figure 5 is a second fixed base provided in the embodiment of the present invention Schematic diagram of the seat structure. The elastic beam 3 , the first fixed base 1 and the second fixed base 2 provided by the embodiment of the present invention will be described in detail below with reference to FIGS. 2-5 .

As shown in FIG. 3 , the elastic beam 3 provided by the embodiment of the present invention includes a first fixing part 301 , a second fixing part 302 and a bending part 303 , and the bending part 303 connects the first fixing part 301 and the second fixing part 302 . The elastic beam 3 is fixedly connected to the first fixed base 1 through the first fixing part 301, the elastic beam 3 is fixedly connected to the second fixed base 2 through the second fixing part 302, and the fiber Bragg grating 4 is fixedly arranged on the fixed base 1. above the curved portion 303. The concave surface of the curved part 303 faces the first card slot 101 and the second card slot 201, and the convex surface faces the direction of 102 and 202. 201 fixed connection.

Specifically, the first fixing part 301 and the second fixing part 302 are straight structures, which are convenient to realize the connection with the first fixing base 1 and the second fixing base 2, and the first fixing part 301 and the second fixing part 302 The distance between them can be selected according to the actual thickness of bivalve organisms such as scallops. In the embodiment of the present invention, the fiber Bragg grating 4 is fixed on the bending part 303 to facilitate the triggering of the fiber Bragg grating 4 . When the shells of bivalve molluscs such as scallops are opened and closed, the first fixed base 1 and the second fixed base 2 are opened and closed, and the first fixed base 1 and the second fixed base 2 that are opened and closed are passed through the elastic beams. The first fixing part 301 and the second fixing part 302 of 3 are transmitted to the bending part 303 of the elastic beam 3, thereby triggering the fiber Bragg grating 4, that is, when the bending deformation occurs in the bending part 303, the bending deformation will cause the The fiber Bragg grating 4 on the 3 is strained synchronously, thereby causing the change of the center wavelength of the fiber Bragg grating 4 . The combination of the first fixing part 301 , the second fixing part 302 and the bending part 303 facilitates more accurate triggering of the fiber Bragg grating 4 , and thus can more accurately measure the frequency characteristics of the opening and closing of the organism, respiration and filter feeding.

Preferably, the fiber Bragg grating 4 is arranged outside the bending portion 303 of the elastic beam 3 , which facilitates the triggering of the fiber Bragg grating 4 and facilitates the installation of the fiber Bragg grating 4 .

As shown in FIG. 4 , in the embodiment of the present invention, a first locking slot 101 is provided on the first fixed base 1 . The elastic beam 3 is engaged with the first fixed base 1 through the first slot 101 . The first fixing portion 301 of the elastic beam 3 is engaged with the first locking groove 101 .

As shown in FIG. 4 , a first hole 102 is provided at an end of the first fixed base 1 far away from the fixedly connected elastic beam 3 . In the embodiment of the present invention, the water pollution monitoring sensor usually uses waterproof glue to fix the first fixed base 1 on the shells of bivalve molluscs such as scallops, so the setting of the first hole 102 is convenient for applying waterproof glue. Strengthen the connection between the shell and the water pollution monitoring sensor, effectively prevent the water pollution monitoring sensor from falling off the shell, and improve the stability of the connection between the water pollution monitoring sensor and the shell. In the embodiment of the present invention, the first hole 102 is a through hole. Preferably, the number of the first holes 102 is two, but not limited to two, and can be selected according to specific usage requirements.

Further, as shown in FIG. 5 , in the embodiment of the present invention, a second card slot 201 is provided on the second fixed base 2 . The elastic beam 3 is engaged with the second fixed base 2 through the second slot 201 . Optionally, the second fixing portion 302 of the elastic beam 3 is engaged with the second locking groove 201 .

As shown in FIG. 5 , a second hole 202 is provided at an end of the second fixed base 2 away from its fixedly connected elastic beam 3 . In the embodiment of the present invention, the water pollution monitoring sensor usually uses waterproof glue to fix the second fixed base 2 on the shells of bivalve molluscs such as scallops, so setting the second hole 202 is convenient for applying waterproof glue and strengthening the shells. The connection with the water pollution monitoring sensor effectively prevents the water pollution monitoring sensor from falling off the shell, and improves the stability of the connection between the water pollution monitoring sensor and the shell. In the embodiment of the present invention, the second hole 202 is a through hole. Preferably, the number of the second holes 202 is two, but not limited to two, and can be selected according to specific usage requirements.

Furthermore, in order to facilitate the fixed connection between the first fixed base 1 and the second fixed base 2 and bivalve mollusks such as scallops, the front ends of the first fixed base 1 and the second fixed base 2 are provided with through holes 102, 202, are used for effectively fixing shell and base, can prevent slipping and loosening.

In the embodiment of the present invention, the water pollution monitoring sensor further includes an optical cable 6 connected to the fiber Bragg grating 4 . The optical cable 6 is used to realize the output of the center wavelength of the fiber Bragg grating 4, which is more convenient for the connection and use of the water pollution monitoring sensor.

Further, an optical cable buffer protection sheath 7 is set at the connection between the fiber Bragg grating 4 and the optical cable 6 , and the optical cable buffer protection sheath 7 is sleeved on the connection joint between the optical fiber Bragg grating 4 and the optical cable 6 . The optical cable buffer protection sleeve 7 is used to protect the connection between the fiber Bragg grating 4 and the optical cable 6 .

Furthermore, in order to facilitate the installation of the optical cable 6, the first fixed base 1 or the second fixed base 2 is provided with an optical fiber groove, and the optical cable buffer protection sleeve 7 is embedded in the optical fiber groove. As shown in Figure 5, the second fixed base 2 is provided with an optical fiber slot 203, the optical fiber slot 203 passes through the second card slot 201, and the optical cable buffer protection sleeve 7 is embedded in the optical fiber slot 203, when the second card slot 201 After the elastic beam 3 is connected, the optical cable 6 is pressed into the optical fiber groove 203 through the optical cable buffer protection sleeve 7 .

Based on the water pollution monitoring sensor provided by the embodiment of the invention, the invention also provides a water pollution monitoring system. Fig. 6 is a schematic structural diagram of a water pollution monitoring system provided in an embodiment of the present invention. As shown in Fig. 6, the water pollution monitoring system provided in the embodiment of the present invention includes a computer 8, a demodulator 9 and several water pollution monitoring sensors. The water pollution monitoring sensor is the water pollution monitoring sensor described in the above embodiments. The signal input end of the demodulator 9 is connected to the output end of the fiber Bragg grating 4 of the water pollution monitoring sensor through an optical cable, and the signal output end of the demodulator 9 is connected to the computer 8 . The computer 8 obtains the change curve of the grating center wavelength of the fiber Bragg grating 4 through the demodulator 9, so as to obtain the characteristic law of the organism's respiration and filter feeding, and then calculate the corresponding water pollution characteristic parameters.

In the embodiment of the present invention, a plurality of water pollution monitoring sensors can be installed in the water pollution monitoring system, and the number of sensors can be expanded according to actual needs. Preferably, the fiber grating reference center wavelengths used by each water pollution monitoring sensor are different, and then wavelength division multiplexing can be realized, that is, a group of water pollution monitoring sensors that include a plurality of sensing probes can share an optical fiber with each other. /Optical cable channel without crosstalk, multiple water pollution monitoring sensors can be connected in parallel through optical fiber splitters or optical fiber couplers, or can be designed in series. Each group of water pollution monitoring sensors is connected through an optical cable for signal transmission. The optical fiber cable is a tensile multi-core optical cable such as Teflon, which can be used not only for signal transmission, but also as a cable for retracting and releasing sensors.

In the water pollution monitoring system provided in the embodiment of the present invention, select bivalve mollusc organisms that are healthy and suitable for survival in the monitored waters, such as scallops, etc., and assemble and package the water pollution monitoring sensor according to Figure 6, bivalve molluscs The organism is stably clamped between the first fixed base 1 and the second fixed base 2 of the water pollution monitoring sensor, and is coated with fast-curing cement or water-resistant glue through the first and second holes reserved for the upper and lower bases , which is conducive to long-term and firm fixation of bivalve organisms on the first fixing base 1 and the second fixing base 2 .

The demodulator 9 is connected to the water pollution monitoring sensor array by connecting the optical fiber cable 6, the demodulator 9 is connected to the computer 8 through a network cable interface, and the computer is provided with data reading software, which can monitor the internal packaging of each water pollution monitoring sensor in real time The center wavelength of the fiber Bragg grating.

As an example, Fig. 7 is a waveform diagram of healthy and sick bivalve organisms before and after water poisoning, and Fig. 8 is a waveform diagram of respiration, filter feeding and dormancy of a certain bivalve organism in a normal water quality environment. Then according to the data in Figure 7 and Figure 8, the change of water quality can be monitored in real time. For example, some mussel shells are open 70-80% of the time to allow access to food and oxygen, but the timing of their shell closure changes in the presence of water pollution.

At the same time, because different types of organisms have different basic biological characteristics, in practical applications, multiple water pollution monitoring sensors are often selected for array deployment, and the types of organisms used are guaranteed to be consistent. In addition, a standard water tank is set up in the water area, and the water quality in the water tank is standard healthy water quality, for example, seawater is healthy seawater water source without pollution, and fresh water is ordinary drinking water source water and other common water without obvious pollution. The data collected by the water pollution monitoring sensors placed in the water tank is set as the standard value signal, and the signals of other sensors are compared with the standard value signal for observation. If the characteristics are the same or similar, the water quality is considered safe, and if there is a huge fluctuation difference, it is judged as abnormal. Multiple water pollution monitoring sensors placed in the same water area refer to each other. If one of the water pollution monitoring sensors is abnormal, for example, when the signal characteristic disappears due to the death of organisms, the other water pollution monitoring sensors have no abnormality, and the abnormality can be ruled out. The water pollution monitoring sensor signal is treated as a failure signal.

In the embodiment of the present invention, several water pollution monitoring sensors are connected in series in the water pollution monitoring system. The series method can use one optical fiber to string several water pollution monitoring sensors. When many sensors are required to be deployed, only one optical cable can be connected in series and combined with wavelength division multiplexing, which greatly reduces the difficulty of wiring and branching. It provides convenience for the deployment of monitoring, especially for actual ocean deployment.

The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and changes. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims (13)

1. A water pollution monitoring biosensor, comprising: the method comprises the following steps:

aquatic organisms, which are living bivalve organisms;

the grating fixing structure comprises a first fixing base, a second fixing base and an elastic beam piece, wherein the elastic beam piece is of an arch structure, and a first fixing part and a second fixing part of the elastic beam piece are respectively arranged on the first fixing base and the second fixing base, so that a bending part in the middle of the elastic beam piece is positioned between the two fixing bases; the two fixed bases are respectively fixed on the surfaces of the two shells of the aquatic organisms;

the grating is a fiber Bragg grating and is adhered to the outer side of the bending part of the grating fixing structure;

the outer side wall of the bending part is positioned in the isolation bin, the isolation bin is formed by surrounding the bending part and a waterproof isolation wall, and isolation liquid is filled in the isolation bin;

the waterproof isolation wall is made of rubber or plastic;

the isolation liquid is corrosion-resistant, low in expansion coefficient and not easy to freeze or vaporize;

the isolation bin is wrapped outside the elastic beam piece and the grating;

one end of the isolation bin is fixed on the first fixed base, and the other end of the isolation bin is fixed on the second fixed base.

2. The water pollution monitoring biosensor as recited in claim 1, wherein: when the bending part is not elastically deformed, the first fixed base is parallel to the second fixed base.

3. The water pollution monitoring biosensor as recited in claim 1, wherein: the first fixing base is provided with a first clamping groove, the second fixing base is provided with a second clamping groove, and the first fixing part and the second fixing part of the elastic beam piece are inserted into the first clamping groove and the second clamping groove respectively.

4. The water pollution monitoring biosensor as recited in claim 3, wherein:

the elastic beam piece is made of stainless steel or fiber composite materials.

5. The water pollution monitoring biosensor as recited in claim 1, wherein: the first fixing base and the second fixing base are bonded on the shell of the bivalve shellfish organism through waterproof glue.

6. The water pollution monitoring biosensor as recited in claim 5, wherein: the first fixing base is provided with a first hole at the bonding position with the shell, the second fixing base is provided with a second hole at the bonding position with the shell, and the first hole and the second hole are through holes.

7. The water pollution monitoring biosensor as recited in claim 1, wherein: a groove body is arranged on the first fixing base or the second fixing base.

8. A water pollution monitoring system, characterized by: the method comprises the following steps:

a water pollution monitoring biosensor as claimed in claim 1;

the demodulator is connected with the grating of the biosensor through an optical cable;

the computer is connected with the demodulator;

a plurality of biosensors are connected in series, and the series monitoring of the biosensors is realized by adopting wavelength division multiplexing.

9. The water pollution monitoring system according to claim 8, wherein: and an optical cable buffer protective sleeve is arranged at the joint of the grating and the optical cable.

10. The water pollution monitoring system according to claim 9, wherein: the optical cable buffer protective sleeve is arranged in the optical fiber groove.

11. The water pollution monitoring system according to claim 9, wherein: the reference center wavelength of the grating used by each sensor is different.

12. The water pollution monitoring system according to claim 8, wherein: the water pollution monitoring device further comprises a standard water tank, and standard water and a water pollution monitoring biosensor are placed in the standard water tank.

13. A method for monitoring water pollution using the water pollution monitoring biosensor of claim 1, wherein: the method comprises the following steps:

the opening and closing movement of the two shells caused by the respiration of the bivalve shellfish organism is transmitted to the elasticity Liang Pianshang through the two fixed bases to cause the relaxation or the compression of the bending part of the elastic beam piece, so that the grating tightly attached to the bending part of the elastic beam piece is synchronously deformed to cause the change of the central wavelength of the grating;

the water quality condition of the water area where the bivalve shellfish organism is located is monitored by monitoring the change rule of the central wavelength of the grating.

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