CN111700927A - A kind of medicine and food homologous composition with hypoglycemic effect and its preparation method and application - Google Patents

Abstract

The invention discloses a medicine and food homologous composition with hypoglycemic effect and a preparation method and application thereof. The composition is prepared by mixing the extracts of Eucommia ulmoides, Alpinia officinalis and Panax notoginseng. The composition comprises the following steps: (1) by the raw materials of Eucommia ulmoides, Cordyceps sinensis and Panax notoginseng, through steps such as drying, pulverizing, sieving to obtain Eucommia, Cordyceps sinensis and Panax notoginseng powder; (2) Eucommia ulmoides, Cordyceps sinensis and Panax notoginseng The Panax notoginseng powder was extracted by solvent, alcohol precipitation, extraction and crystallization to obtain the extracts of Eucommia ulmoides, Cordyceps sinensis and Panax notoginseng. ‑1:1:3 method to mix and shake to prepare the combined extract. The medicine and food homologous composition produced by the invention can significantly reduce the blood sugar index and postprandial blood sugar content of diabetic patients, has good preventive and therapeutic effects on complications caused by diabetes, and has significant social and economic benefits.

Description

A kind of medicine and food homologous composition with hypoglycemic effect and its preparation method and application

technical field

The invention belongs to the technical field of extraction and application of plant effective active ingredients, and in particular relates to a medicine-food homologous composition with hypoglycemic effect and a preparation method and application thereof.

Background technique

Diabetes mellitus refers to a disease of glucose metabolism disorder caused by insufficient insulin secretion, insulin action disorder, glucose metabolism disorder, or a combination of the three. Its symptoms are polydipsia, polyphagia, polyuria and weight loss. Diabetes is one of the largest global health emergencies of the 21st century with a rising global prevalence and currently affects 8.8% of adults. Diabetes can induce a variety of acute or chronic complications, such as cardiovascular and cerebrovascular and diabetic nephropathy. Persistent glucose and lipid metabolism disorders can lead to many diseases in large and small blood vessels, microvessels, nervous systems, etc., resulting in abnormal tissue structure and function. Diabetes is known in the medical community as "the source of all diseases" and endangers the health of people around the world. There are various types of hypoglycemic drugs on the market. Most of these drugs are chemically or biologically synthesized. Although the hypoglycemic effect is obvious, they also bring many side effects. At the same time, the expensive price is also prohibitive. In recent years, researches on extracting active ingredients from natural products to prevent and treat diabetes have become a hot topic. Therefore, it is a great development trend to find high-efficiency, low-toxicity, and cheap natural medicines and health foods from the resource-rich traditional Chinese medicines.

The scientific name of Mansanqi is "Gynura procumbens (Lour.) Merr.", which belongs to the Compositae family and belongs to the genus of Panax notoginseng. Cool, a perennial herbal medicinal and edible plant, widely distributed in China, Malaysia, Thailand, Indonesia, South Korea and the Philippines and other countries. Recumbent chrysanthemum Panax notoginseng has a wide range of pharmacological effects. In the first half of 2009, relevant units such as China Center for Disease Control and Prevention, National Center for Food Quality Supervision and Inspection, China Food and Fermentation Industry Research Institute and other relevant units completed the toxicology test, sanitation The results of scientific testing and testing of various components are as follows: non-toxic and non-teratogenic; its active ingredients have the functions of dredging meridians and activating collaterals, reducing inflammation and relieving cough, dispersing stasis and swelling, promoting blood circulation and promoting muscle growth, treating traumatic injuries, bronchial pneumonia, tuberculosis, etc. Efficacy; can delay aging, activate immune cells, improve immune function, enhance human immunity, enhance human metabolism and improve memory impairment; it has blood pressure, blood lipid, blood sugar, anti-oxidation, anti-ulcer and prevention. It has significant effects on chronic kidney disease and hepatitis B inhibition; it has certain curative effects on the prevention and treatment of cardiovascular and cerebrovascular diseases, diabetes, etc. On the other hand, Chrysanthemum Panax notoginseng is a unique plant that can be used for both medicine and food, and has excellent nutritional value. Therefore, it is widely used in the food and pharmaceutical industry, daily chemical industry and other fields. High economic value of medicinal and edible plants.

Lithocarpus polystachyus Rehd, also known as Lithocarpus polystachyus Rehd. Most of them are distributed in the provinces south of the Yangtze River in the wild state, and the resources are most concentrated in Jiangxi, Hunan, Fujian, Guangxi, Anhui and other provinces. Phloridzin and trilobulin are the main components, and the content is as high as 9.0% to 14.60%. Modern medical research has shown that Cordyceps sinensis has the functions of clearing heat and diuresis, moistening the lungs and relieving cough, moisturizing the liver and kidney, etc. It can be used to prevent and treat warm dysentery, carbuncle malignant sores, skin itching, etc. effect. Modern research has shown that Cordyceps sinensis has antioxidant, hypoglycemic, hypolipidemic, antibacterial, anti-inflammatory, anti-allergic and other pharmacological effects.

Eucommia ulmoides Oliver, also known as bakelite, is a plant of the Eucommia family. It is a unique and precious nourishing Chinese medicinal material in my country with high medicinal value. Modern pharmacological studies have shown that Eucommia has anti-aging, anti-oxidation, lowering blood pressure, lowering blood lipids, lowering blood sugar, anti-mutagenic, anti-cancer, antibacterial and antiviral effects. Eucommia is rich in active ingredients, mainly including flavonoids, phenylpropanoids, iridoids, polysaccharides, polyphenols, phenylpropanoids, lignans, etc., and Eucommia has prominent pharmacological effects and has a variety of health care benefits. effect. The whole body of Eucommia is a treasure, and its seeds, flowers, skins, and leaves can be eaten. After toxicological identification, Eucommia seed oil and Eucommia male flower are safe and non-toxic. Both have been approved as new food raw materials by the National Health and Family Planning Commission. Eucommia ulmoides leaves were also approved by the National Health and Health Commission in 2019 to be listed as homologous to human medicine and food. In the pilot list, there are great prospects in the development of healthy food.

SUMMARY OF THE INVENTION

The object of the present invention is to provide a medicine and food homologous composition with hypoglycemic effect and its preparation method and application, which utilizes different extraction and purification methods to enrich the active ingredients in Eucommia ulmoides, Alpinia officinalis and Panax notoginseng respectively. , and the obtained powders are combined by optimizing the combination, and the composition is tested for hypoglycemic activity. Compared with a single extract, the effects are complemented, and the hypoglycemic effect can be synergistically improved.

For realizing the above-mentioned purpose of the invention, the present invention adopts following technical scheme:

A medicinal and food homologous composition with hypoglycemic effect, respectively using Eucommia, Alpinia officinalis and Panax notoginseng as raw materials, extracting Eucommia ulmoides extract, Alpinia officinalis extract, Panax notoginseng extract, and then The Eucommia ulmoides extract, the Cordyceps sinensis extract and the Panax notoginseng extract are mixed in a mass ratio of 1:1:1-1:1:3 to obtain a combined extract.

Further preferably, the combined extract needs to contain chlorogenic acid, cryptochlorogenic acid, caffeic acid, geniposide, phytoside, isochlorogenic acid B, catechin, isoquercitrin, isogreen Orthoic acid A, phloridzin, pinoresinol diglucoside, isochlorogenic acid C, treloin and rutin.

A preparation method of a medicine-food homologous composition with hypoglycemic effect, comprising the following steps:

Step 1): collecting Eucommia ulmoides leaves as raw materials, using water as extraction solvent after drying and pulverizing, the extraction temperature is 80 ° C, the material-liquid ratio is 1:10-20, stirring and extracting, filtering, and the combined filtrate is concentrated under reduced pressure to 1/1 of the original solution. 10. Add 4 times the amount of absolute ethanol, put it in the refrigerator, overnight, collect the supernatant by centrifugation, concentrate until there is no alcohol smell, add an equal volume of distilled water to ultrasonically disperse, add an equal volume of solvent for extraction, extract three times, and combine the extraction phases. , concentrated to dryness to prepare Eucommia ulmoides leaf extract;

Step 2): collecting Cordyceps sinensis as raw material, using water as extraction solvent after drying and pulverizing, the extraction temperature is 70 ° C, the ratio of material to liquid is 1:10-20, stirring and extracting, filtering, and the combined filtrate is concentrated under reduced pressure to 1 of the original solution. /10, add 10% activated carbon at 70°C and stir to decolorize, and the filtrate is spray-dried to obtain the extract of Cordyceps sinensis;

Step 3): collecting the Panax notoginseng leaves as raw materials, using an aqueous ethanol solution as the extraction solvent after drying and pulverizing, collecting the supernatant by centrifugation, concentrating until there is no alcohol smell, and concentrating to dryness to prepare the Panax notoginseng leaf extract;

Step 4): Mix and shake the Eucommia ulmoides extract, the Cordyceps sinensis extract, and the Panax notoginseng extract according to the proportions to prepare a medicine and food homologous composition.

Further, in step 4), the extract of Eucommia ulmoides, the extract of Cordyceps sinensis, and the extract of Panax notoginseng are mixed in a mass ratio of 1:1:1-1:1:3 to obtain a combined extract.

Further, the composition extract needs to contain chlorogenic acid, cryptochlorogenic acid, caffeic acid, geniposide, phytoside, isochlorogenic acid B, catechin, isoquercitrin, isogreen Orthoic acid A, phloridzin, pinoresinol diglucoside, isochlorogenic acid C, treloin and rutin.

Further, in step 3), the extraction solvent is 50-90% ethanol aqueous solution with PH=2-7, the liquid-material ratio is 1:10-20, the extraction temperature is normal temperature, the ultrasonic extraction is performed for 2 hours, and the filtrate is concentrated under reduced pressure to No alcohol smell.

Further, the organic solvent used in step 1) is any one of petroleum ether, n-hexane, dichloromethane, ethyl acetate, n-butanol, dichloromethane/methanol and petroleum ether/ethyl acetate.

Further, preferably, the extraction solvent used in step 1), the selected extraction solvent is ethyl acetate/methanol, and the ethyl acetate/methanol volume ratio of 4:1 is the best.

The invention also provides the application of the prepared medicine and food homologous composition in the preparation of diabetes medicine.

Due to adopting the above-mentioned technical scheme, the present invention has the following advantages:

The present invention enriches the effective components of Eucommia ulmoides, Cordyceps sinensis and Panax notoginseng through different extraction and purification methods, and obtains a total of 14 effective components, which are chlorogenic acid, cryptochlorogenic acid, caffeic acid, and genipin. Glycosides, phytoside, isochlorogenic acid B, catechin, isoquercitrin, isochlorogenic acid A, phlorizin, pinoresinol diglucoside, isochlorogenic acid C, trilobin and rutin , and its content accounts for 90% of the composition powder, which reveals the material basis for lowering blood sugar. Compared with traditional Chinese medicine, it more effectively enriches the active ingredients and discards some ineffective ingredients. The prepared medicine and food homologous composition has a particularly good effect on diabetic patients, and the patients who have used the extract basically have no recurrence of gout, and the effect is obviously better than the single use of Eucommia ulmoides, Alpinia officinalis and Panax notoginseng extracts. thing.

Description of drawings

Figure 1 is a liquid chromatogram of the composition of the present invention.

Fig. 2 is the liquid chromatogram of the Panax notoginseng extract of the present invention.

Figure 3 is a liquid chromatogram of the Eucommia ulmoides extract of the present invention.

Fig. 4 is the liquid chromatogram of the Cordyceps sinensis extract of the present invention.

Fig. 5 is a liquid chromatogram of the extract of the comparative example of the present invention.

Detailed ways

The present invention can be further described in detail with reference to the following examples; however, the following examples are merely illustrative, and the present invention is not limited to these examples.

A preparation method of a medicine-food homologous composition with hypoglycemic effect, comprising the following steps:

Step 1): collecting Eucommia ulmoides leaves as raw materials, using water as extraction solvent after drying and pulverizing, the extraction temperature is 80 ° C, the material-liquid ratio is 1:10-20, stirring and extracting, filtering, and the combined filtrate is concentrated under reduced pressure to 1/1 of the original solution. 10. Add 4 times the amount of absolute ethanol, put it in the refrigerator, overnight, collect the supernatant by centrifugation, concentrate until there is no alcohol smell, add an equal volume of distilled water to ultrasonically disperse, add an equal volume of solvent for extraction, extract three times, and combine the extraction phases. , concentrated to dryness to prepare Eucommia ulmoides leaf extract;

Step 2): collecting Cordyceps sinensis as raw material, using water as extraction solvent after drying and pulverizing, the extraction temperature is 70 ° C, the ratio of material to liquid is 1:10-20, stirring and extracting, filtering, and the combined filtrate is concentrated under reduced pressure to 1 of the original solution. /10, add 10% activated carbon at 70°C and stir to decolorize, and the filtrate is spray-dried to obtain the extract of Cordyceps sinensis;

Step 3): collecting the Panax notoginseng leaves as raw materials, using alcohol water as the extraction solvent after drying and pulverizing, collecting the supernatant by centrifugation, concentrating until there is no alcohol smell, and concentrating to dryness to prepare the Panax notoginseng leaf extract;

Step 4): Mix and shake the Eucommia ulmoides extract, the Cordyceps sinensis extract, and the Panax notoginseng extract according to the proportions to prepare a medicine and food homologous composition. The combined extract contains chlorogenic acid, cryptochlorogenic acid, caffeic acid, geniposide, phytoside, isochlorogenic acid B, catechin, isoquercitrin, isochlorogenic acid by liquid phase detection A. Phloridzin, Pinoresinol Diglucoside, Isochlorogenic Acid C, Trilobin and Rutin.

Example 1

Take 100 g of Eucommia ulmoides leaves, dry and pulverize, use water as the extraction solvent, the extraction temperature is 80 ° C, the material-liquid ratio is 1:20, stir and extract, filter, the combined filtrate is concentrated under reduced pressure to 1/10 of the original solution, and 4 times the amount of Absolute ethanol, put in the refrigerator, overnight, centrifuged to collect the supernatant, concentrated until there is no alcohol smell, add equal volume of distilled water to ultrasonically disperse, add equal volume of n-butanol for extraction, extract three times, combine the extract phases, and concentrate to dryness. 2.35 g of Eucommia ulmoides leaf extract was obtained, with a yield of 2.35%. The liquid chromatogram of the Eucommia ulmoides extract is shown in FIG. 3 .

Take 100 g of Cordyceps sinensis, dry and pulverize, and use water as the extraction solvent, the extraction temperature is 70 ° C, and the ratio of material to liquid is 1:20, stirring and extracting, filtering, the combined filtrate is concentrated under reduced pressure to 1/10 of the original solution, and 10% of activated carbon is added. Stirring at 70° C. for decolorization, and the filtrate was spray-dried to obtain 6.42 g of the extract of Cordyceps sinensis, with a yield of 6.42%.

Take 100 g of Panax notoginseng leaves, dry and pulverize, use 50% ethanol aqueous solution with pH=3, the material-liquid ratio is 1:10, the extraction temperature is normal temperature, ultrasonic extraction is carried out for 2 hours, and the filtrate is concentrated under reduced pressure until there is no alcohol smell. Concentrating to dryness to prepare 9.24 g of the Panax notoginseng leaf extract, the yield is 9.24%, and the liquid chromatogram of the Panax notoginseng extract is shown in Figure 2 .

The eucommia ulmoides extract, the extract of Cordyceps sinensis, and the extract of Panax notoginseng are mixed and shaken in a mass ratio of 1:1:1 to prepare a medicine-food homologous composition. The liquid phase detection in the combined extract contains 1 chlorogenic acid, 2 cryptochlorogenic acid, 3 caffeic acid, 4 geniposide, 5 phytoside, 6 isochlorogenic acid B, 7 catechin, 8 isochlorogenic acid Quercitrin, 9 isochlorogenic acid A, 10 phloridzin, 11 pinoresinol diglucoside, 12 isochlorogenic acid C, 13 trilobulin and 14 rutin and other components, as shown in Figure 1.

Example 2

Take 100 g of Eucommia ulmoides leaves, use water as the extraction solvent after drying and pulverizing, the extraction temperature is 80 ° C, the material-liquid ratio is 1:10, stir and extract, filter, combine the filtrate and concentrate under reduced pressure to 1/10 of the original solution, add 4 times the amount of Absolute ethanol, put in the refrigerator, overnight, centrifuged to collect the supernatant, concentrated until there is no alcohol smell, add equal volume of distilled water to ultrasonically disperse, add equal volume of dichloromethane/methanol for extraction, extract three times, combine the extract phases, and concentrate to Dry prepared Eucommia ulmoides leaf extract 4.18g, yield 4.18%;

Take 100 g of Cordyceps sinensis, dry and pulverize, use water as the extraction solvent, the extraction temperature is 70 ° C, the material-to-liquid ratio is 1:10, stir and extract, filter, the combined filtrate is concentrated under reduced pressure to 1/10 of the original solution, and 10% of activated carbon is added. Stirring at 70° C. for decolorization, the filtrate was spray-dried to obtain 4.25 g of the extract of Cordyceps sinensis, with a yield of 4.25%.

Take 100 g of Panax notoginseng leaves, dry and pulverize, use 80% ethanol aqueous solution with pH=2, the material-to-liquid ratio is 1:20, the extraction temperature is normal temperature, ultrasonic extraction is performed for 2 hours, and the filtrate is concentrated under reduced pressure until there is no alcohol smell. Concentrate to dryness to prepare 8.77 g of the extract of Panax notoginseng leaves, and the yield is 8.77%.

The eucommia ulmoides extract, the extract of Cordyceps sinensis, and the extract of Panax notoginseng are mixed according to the mass ratio of 1:1:2, and shaken to prepare a medicine-food homologous composition. The combined extract contains chlorogenic acid, cryptochlorogenic acid, caffeic acid, geniposide, phytoside, isochlorogenic acid B, catechin, isoquercitrin, isochlorogenic acid by liquid phase detection A. Phloridzin, Pinoresinol Diglucoside, Isochlorogenic Acid C, Trilobin and Rutin.

Example 3

Take 100 g of Eucommia ulmoides leaves, dry and pulverize, use water as the extraction solvent, the extraction temperature is 80 ° C, the material-liquid ratio is 1:15, stir and extract, filter, the combined filtrate is concentrated under reduced pressure to 1/10 of the original solution, and 4 times the amount of Absolute ethanol, put in the refrigerator, overnight, centrifuged to collect the supernatant, concentrated until there is no alcohol smell, add equal volume of distilled water to ultrasonically disperse, add equal volume of petroleum ether/ethyl acetate for extraction, extract three times, combine the extract phases, and concentrate To dryness, 2.04 g of Eucommia ulmoides leaf extract was prepared, and the yield was 2.04%.

Take 100 g of Cordyceps sinensis, use water as extraction solvent after drying and pulverizing, the extraction temperature is 70 ° C, the ratio of material to liquid is 1:15, stirred and extracted, filtered, the combined filtrate was concentrated under reduced pressure to 1/10 of the original solution, and 10% of activated carbon was added. Stirring and decolorizing at 70° C., the filtrate was spray-dried to obtain 7.44 g of the extract of Cordyceps sinensis, with a yield of 7.44%.

Take 100 g of Panax notoginseng leaves, dry and pulverize, use a 60% ethanol aqueous solution with pH=7, the material-to-liquid ratio is 1:15, the extraction temperature is normal temperature and ultrasonic extraction for 2 hours, and the filtrate is concentrated under reduced pressure until there is no alcohol smell. Concentrate to dryness to prepare 10.45 g of the extract of Panax notoginseng leaves, and the yield is 10.45%.

The eucommia ulmoides extract, the extract of Cordyceps sinensis, and the extract of Panax notoginseng are mixed and shaken in a mass ratio of 1:1:3 to prepare a medicine-food homologous composition. The combined extract contains chlorogenic acid, cryptochlorogenic acid, caffeic acid, geniposide, phytoside, isochlorogenic acid B, catechin, isoquercitrin, isochlorogenic acid by liquid phase detection A. Phloridzin, Pinoresinol Diglucoside, Isochlorogenic Acid C, Trilobin and Rutin.

Comparative ratio

Take 100 g each of Eucommia ulmoides leaves, Cordyceps sinensis and Pseudomonas notoginseng, pulverize them together with a pulverizer, and add them to a conical flask. The extraction solvent is a 90% ethanol aqueous solution with pH=5, the material-to-liquid ratio is 1:15, and the extraction temperature is Extracted at room temperature and ultrasonic for 2 hours, filtered, and the filtrate was concentrated under reduced pressure until there was no alcohol smell. Add 4 times the volume of 100% ethanol solution to the obtained solution, put it in a 4°C refrigerator overnight for alcohol precipitation, collect the supernatant by centrifugation, and concentrate under reduced pressure until there is no alcohol odor to obtain a mixed extract, which is subjected to solvent extraction. , the extraction solvent used was n-butanol, the extracts were combined, concentrated under reduced pressure, and dried to obtain 4.52 g of a mixed extract containing chlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, phloridzin , isochlorogenic acid C and rutin. Compounds cryptochlorogenic acid, caffeic acid, geniposide, phytoside, catechin, isoquercitrin, pinoresinol diglucoside and trefoil were not detected.

Effects of medicinal and food homologous compositions on blood glucose in mice induced by streptozotocin

130 healthy male Kunming mice (about 20g) were selected as experimental animals and randomly divided into 13 groups: (blank control group, model control group, positive control group (acarbose), mixed extract = 1:1:1 High-dose group, mixed extract=1:1:1 Low-dose group, mixed extract=1:1:2 High-dose group, mixed extract=1:1:2 Low-dose group, mixed extract=1:1 : 3 high-dose group, mixed extract = 1:1:3 low-dose group, Radix chinensis extract group, Panax notoginseng extract group, Eucommia ulmoides leaf extract group, control group) 10 in each group. In the first week, ordinary feed was used to provide sufficient food and water to avoid abnormalities caused by environmental changes. One week later, all mice were fed with water quickly, and 12 hours later, 0.1 mol/L STZ solution was injected into the abdomen outside the blank control group. The injection dose of each mouse was selected to be 60 mg/kg to establish a model of type 2 diabetes, and the blank control group was injected with an equal volume of distilled water. After injection, mice in the blank control group were fed a normal chow diet, and the remaining mice were fed a high-sugar and high-fat diet for 5 days. After 5 days of modeling, the blood glucose of the mice was tested, and a blood glucose range of 11-25 mmol/L was considered successful. If there are mice with blood glucose values less than 11 mmol/L, supplementary injection of STZ is required, and STZ is dissolved in citrate buffer between pH 4.2 and 4.5. And store it in a test tube wrapped in foil. And in 30min rapid injection into the abdominal cavity of mice. The data results are expressed as mean ± standard deviation (x ± s), and the differences between groups are expressed by one-way analysis of variance through the statistical software package SPSS version 19.0. sexual differences.

Mice with blood glucose concentration within 11-25 mmol/L after modeling were evenly divided into 13 groups according to body weight and blood glucose concentration, and were given intragastric administration according to Table 1.

Table 1 Animal experiment grouping

As shown in Table 2, it can be seen from the table that the fasting blood glucose levels of the diabetic mice were significantly increased after successful modeling. And maintain high blood sugar level, compared with blank control group, there is a very significant difference (p<0.01), STZ successfully induced and simulated the hyperglycemia model of mouse diabetes. During the entire administration period, the average blood glucose level of the blank control group was stable at 5 mmol/L, while the mice in the model control group gradually increased with the administration period. It indicated that the condition of diabetes was gradually serious until the fourth and fifth weeks, which remained at about 25mmol/L, and there was a significant or extremely significant difference with the low and high dose groups of the composition (P<0.01 or P<0.05).

In positive control group and mixed extract=1:1:1 high dose group, mixed extract=1:1:1 low dose group, mixed extract=1:1:2 high dose group, mixed extract=1:1 1:2 low-dose group, mixed extract=1:1:3 high-dose group, mixed extract=1:1:3 low-dose group, the blood glucose level of mice in the first three weeks increased with the treatment period . However, it was slower than the model control group. From the 4th week, the positive control group significantly reduced the fasting blood glucose level of the diabetic mice; after five weeks of gavage, the blood glucose of the positive control group decreased by 45.54% compared with the model control group, a significant difference.

Mixed extract=1:1:1 high-dose group, mixed extract=1:1:2 high-dose group, mixed extract=1:1:3 high-dose group Fasting blood glucose values of mice started from the fourth week of gavage There were different degrees of decline, and compared with the model control group, there was a significant or extremely significant difference ((P<0.01 or P<0.05), after 5 weeks of gavage, mixed extract = 1:1:1 high-dose group , mixed extract = 1:1:2 high-dose group, mixed extract = 1:1:3 high-dose group, the blood sugar of mice in the high-dose group decreased by an average of 80% compared with the blood sugar of the model group mice, which can prove that the composition may have effects on diabetes. certain control.

The fasting blood glucose values of the mice in the Mice extract group, the Periphora Radix Notoginseng extract group, the Eucommia ulmoides leaf extract group, and the control group showed the same trend as the high and low dose groups of the mixed extracts. In the fourth and fifth weeks, the effect of the extract group, the Radix Notoginseng extract group, and the Eucommia ulmoides leaf extract group was worse than that of the mixed extract group, but the Radix Radix et Rhizoma extract group, the Radix Radix Notoginseng In the extract group and the Eucommia ulmoides leaf extract group, the fasting blood glucose values of the mice in the fourth and fifth weeks were lower than those in the control group, indicating that at the same dose, the effect of the mixed extract was better than that of the three medicines and food homology alone. The plant extract, the comparative example had the worst effect.

Table 2 Effects of mixed extracts on blood glucose levels in diabetic mice

^## P<0.01 compared with normal control group; ^* P<0.05, ^** P<0.01 and ^*** P<0.001 compared with model group.

The above descriptions are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention shall be included in the protection of the present invention. within the range.

Claims (8)

1. a medicine-food homologous composition with hypoglycemic effect and its preparation method and application, it is characterized in that: composition is respectively raw material with Eucommia ulmoides, Cordyceps sinensis and Periflora Radix Notoginseng, extracts Eucommia ulmoides extract, Eucommia ulmoides respectively. Ginger leaf extract, Periflora Radix Notoginseng extract, and then the Eucommia ulmoides extract, Alpinia officinalis extract, Periflora Radix Notoginseng extract are mixed in a ratio of 1:1:1-1:1:3 to obtain a combined extract. 2. a kind of medicine and food homologous composition with hypoglycemic effect according to claim 1 and its preparation method and application, it is characterized in that: comprise 1 chlorogenic acid, 2 cryptochlorogenic acid, 3 coffee in the combined extract acid, 4 geniposide, 5 phytoside, 6 isochlorogenic acid B, 7 catechin, 8 isoquercitrin, 9 isochlorogenic acid A, 10 phlorizin, 11 pinoresinol diglucoside , 12 isochlorogenic acid C, 13 triloboside and 14 rutin. 3. a medicine-food homologous composition with hypoglycemic effect and its preparation method and application, is characterized in that, comprises the following steps: Step 1): collecting Eucommia ulmoides leaves as raw materials, using water as extraction solvent after drying and pulverizing, the extraction temperature is 80 ° C, the liquid-material ratio is 1:10-20, stirring and extracting, filtering, and the combined filtrate is concentrated under reduced pressure to 1/1 of the original solution. 10. Add 4 times the amount of absolute ethanol, put it in the refrigerator, overnight, collect the supernatant by centrifugation, concentrate until there is no alcohol smell, add an equal volume of distilled water to ultrasonically disperse, add an equal volume of solvent for extraction, extract three times, and combine the extraction phases. , concentrated to dryness to prepare Eucommia ulmoides leaf extract; Step 2): collecting Cordyceps sinensis as raw material, using water as extraction solvent after drying and pulverizing, the extraction temperature is 70 ° C, the ratio of liquid to material is 1:10-20, stirring and extracting, filtering, and the combined filtrate is concentrated under reduced pressure to 1 of the original solution. /10, add 10% activated carbon at 70°C and stir to decolorize, and the filtrate is spray-dried to obtain the extract of Cordyceps sinensis; Step 3): collecting the Panax notoginseng leaves as raw materials, using alcohol water as the extraction solvent after drying and pulverizing, collecting the supernatant by centrifugation, concentrating until there is no alcohol smell, and concentrating to dryness to prepare the Panax notoginseng leaf extract; Step 4): Mix and shake the Eucommia ulmoides extract, the Cordyceps sinensis extract, and the Panax notoginseng extract according to the proportions to prepare a medicine and food homologous composition. 4. the medicine-food homologous composition with hypoglycemic effect according to claim 1 and its preparation method and application, it is characterized in that, in step 4), by Eucommia ulmoides extract, Cordyceps sinensis extract, Chrysanthemum The seven extracts are mixed in a ratio of 1:1:1-1:1:3 to obtain a combined extract. 5. the medicine-food homologous composition with hypoglycemic effect according to claim 1 and its preparation method and application, it is characterized in that, need to comprise 1 chlorogenic acid, 2 cryptochlorogenic acid, 3 caffeic acid, 4 geniposide, 5 phylloside, 6 isochlorogenic acid B, 7 catechin, 8 isoquercitrin, 9 isochlorogenic acid A, 10 phlorizin, 11 pinoresinol two Glucoside, 12-isochlorogenic acid C, 13-triloside and 14-rutin. 6. the medicine-food homologous composition with hypoglycemic effect according to claim 1 and its preparation method and application, it is characterized in that, in step 3), extraction solvent is 50-90% ethanol of PH=2-7 Aqueous solution, the ratio of liquid to material is 1:10-20, the extraction temperature is normal temperature and ultrasonic extraction is performed for 2 hours, and the filtrate is concentrated under reduced pressure until there is no alcohol smell. 7. the medicine-food homologous composition with hypoglycemic effect according to claim 1 and its preparation method and application, it is characterized in that, organic solvent used in step 1) is petroleum ether, n-hexane, dichloromethane, acetic acid Any of ethyl ester, n-butanol, dichloromethane/methanol and petroleum ether/ethyl acetate. 8. The application of the medicine and food homologous composition prepared in any one of claims 1 to 7 in the preparation of diabetes medicine.

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