CN111557968A - A kind of traditional Chinese medicine compound composition and application with regulating immune function - Google Patents

Abstract

The invention discloses a traditional Chinese medicine compound composition and application with immune regulation function, which is composed of the following raw materials by weight: 25-35 g of astragalus, 5-15 g of ginseng, 5-15 g of Ganoderma lucidum, 10-20 g of Banzhilian, 10-20 g of Atractylodes Rhizoma 10‑20g, licorice 10‑20g. In the traditional Chinese medicine compound composition formula provided by the present invention, astragalus and ginseng are the monarch medicine, tonifying qi and raising yang, and strengthening the pulse; Ganoderma lucidum and atractylodes can both strengthen the spleen and replenish qi, and support the righteousness, and are the minister medicine, and Banzhilian is the auxiliary medicine. Astragalus and ginseng can improve the body's immunity, and can restrain the heat and anti-tumor of Astragalus, as adjuvant. The above-mentioned six herbs complement each other and have the effects of strengthening the body and eliminating pathogenic factors, as well as tonifying deficiency. Modern pharmacology shows that the traditional Chinese medicine compound composition of the present invention can increase the weight of spleen lymphocytes, improve the proliferation of spleen lymphocytes and the level of IL-2 secretion, thereby enhancing tumor patients. The effect of immunity, prolong the survival period of tumor patients and improve the quality of life.

Description

A kind of traditional Chinese medicine compound composition and application with regulating immune function

technical field

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine compound composition and application with an immune-regulating function.

Background technique

The current treatment of tumors is mainly based on Western medicine, such as surgery, chemotherapy, radiotherapy, targeted therapy, immunotherapy, etc. However, surgery often has complications, and chemotherapy and radiotherapy often have toxic and side effects. The theory of traditional Chinese medicine believes that the imbalance of yin and yang and the prosperity of positive and deficient evil are the key reasons for the occurrence and development of tumors. Adjusting yin and yang, strengthening the righteousness and eliminating pathogenic factors are the key points of tumor treatment. Traditional Chinese medicine emphasizes that by adjusting yin and yang, and supporting righteousness to achieve a balance between yin and yang, and sufficient righteousness, so as to achieve the purpose of controlling tumor growth, improving patients' clinical symptoms, and improving patients' quality of life.

Therefore, it is extremely necessary to develop a traditional Chinese medicine with the functions of strengthening the body and eliminating pathogenic factors and tonic for deficiency, so as to regulate the immune function of the patient and inhibit the growth of the patient's tumor cells.

SUMMARY OF THE INVENTION

In view of the above problems, the present invention provides a traditional Chinese medicine compound composition with regulating immune function. quality.

The purpose of this invention is to realize through the following technical solutions:

A traditional Chinese medicine compound composition with immune regulation function, which is composed of the following raw materials by weight: 25-35g of Astragalus, 5-15g of Ginseng, 5-15g of Ganoderma Lucidum, 10-20g of Banzhilian, 10-20g of Atractylodes Rhizoma, 10g of Licorice -20g.

Preferably, the traditional Chinese medicine compound composition for regulating immune function is composed of the following raw materials by weight: Astragalus 30g, Ginseng 10g, Ganoderma lucidum 10g, Banzhilian 15g, Atractylodes Rhizoma 15g, and Licorice 10g.

Preferably, the traditional Chinese medicine compound composition for regulating immune function is composed of the following raw materials by weight: Astragalus 25g, ginseng 5g, Ganoderma lucidum 5g, Banzhilian 10g, Atractylodes 10g, and licorice 15g.

The invention also provides the application of the traditional Chinese medicine compound composition for regulating immune function in the preparation of anti-tumor traditional Chinese medicine preparations, wherein the traditional Chinese medicine preparations are decoctions, granules, tablets or pills.

Preferably, the preparation method of described decoction is as follows:

1) According to the proportion, decoct and extract astragalus, ginseng, Ganoderma lucidum, bark lotus, Atractylodes Rhizoma, and licorice for 3 times, 40-60min each time, add 8-12 times the amount of water for the first time, and add 6-10 times the amount of water for the second time. times the amount of water, add 4-8 times the amount of water for the third time to obtain a mixed solution;

2) The mixed solution is filtered through a 500-550 mesh sieve and then sterilized and bottled at high temperature to obtain the target substance.

Preferably, the preparation method of described granule, tablet or pill is as follows:

S1. Decoct and extract astragalus, ginseng, ganoderma lucidum, bark lotus, Atractylodes rhizome, and licorice in proportion for 3 times, 40-60min each time, add 8-12 times the amount of water for the first time, and add 6-10 times the amount of water for the second time. amount of water, add 4-8 times the amount of water for the third time, filter the extract, combine the filtrates, and concentrate to an extract with a relative density of 1.30-1.35;

S2. Add excipients to the extract, mix well, add 0-100ml of 75% ethanol to granulate, and dry at 60°C to 65°C to prepare granules, tablets or pills.

Preferably, the auxiliary materials are substances in the following weight ratios: 15-25g sucrose powder and 70g-75g dextrin.

Solution:

Astragalus: sweet, mainly enters the lung, spleen, and heart meridians, has the functions of invigorating qi and raising yang, invigorating the body and strengthening the table, diuretic and swelling, and nourishing the muscles. It can enhance heart function, improve human immunity, anti-aging, and antibacterial effects. .

Ginseng: flat, sweet, slightly bitter, slightly warm. Returns the spleen, lung, and heart meridians. Efficacy: invigorating vitality, strengthening the pulse, invigorating the spleen and benefiting the lung, promoting body fluid and quenching thirst, soothing the mind and improving wisdom. Indications: overwork injury, deficiency of qi, blood and body fluid.

Ganoderma lucidum: nourishes qi and blood, reassures the mind, and strengthens the spleen and stomach. Indications for consumptive fatigue, palpitations, insomnia, dizziness, fatigue, chronic cough and asthma, coronary heart disease, silicosis, and tumors.

Atractylodes: sweet and bitter in taste, warm in nature, belonging to the spleen and stomach meridians. The main functions are to invigorate Qi and invigorate the spleen, dry dampness and diuretic water, stop sweating and soothe the miscarriage. It can be used to treat spleen deficiency and other symptoms. It can invigorate the spleen and dry dampness. It can be used to treat phlegm-drinking internal stoppage and phlegm-dampness boiling in spleen caused by spleen deficiency and yang disorder.

Banzhilian: Pungent in taste, cold in nature, flat in nature. Returns the lung, liver, and kidney meridians. It has the functions of clearing away heat and detoxifying, promoting blood circulation and removing blood stasis, reducing swelling and relieving pain, and anti-cancer; it can treat appendicitis, hepatitis, stomach pain, early liver cancer, lung cancer, cervical cancer, mastitis, etc.;

Licorice tonifies the middle and replenishes the Qi and harmonizes the various medicines as the medicine.

In this recipe, astragalus and ginseng are the monarch medicine, tonifying qi and raising yang, and strengthening the pulse; both Ganoderma lucidum and Atractylodes can strengthen the spleen and qi, and support righteousness. , and can contain Qishen warm, anti-tumor, as an adjuvant, licorice tonifying Qi and reconciling various medicines as the medicine; the above six herbs complement each other, strengthen the righteousness and dispel pathogens.

The immune influence of the traditional Chinese medicine compound composition provided by the present invention on the tumor body refers to the following test:

experiment method:

1. Establishment, grouping and administration of H22 tumor-bearing mouse model

S180 mouse-derived mice were selected for ⁸ days of inoculation. After the abdominal skin was disinfected, ascites was extracted, and the supernatant was discarded after centrifugation. On the next day, the inoculated mice were randomly divided into 4 groups, namely the tumor-bearing model group, the CTX positive group, the traditional Chinese medicine compound composition of the present invention and the CTX combined with compound astragalus oral liquid group, with 8 mice in each group. The CTX positive group was intraperitoneally injected with cyclophosphamide 30 mg/kg, and the traditional Chinese medicine compound composition of the present invention was administered by gavage at a dose of 100 mg/kg in the form of decoction. The CTX combined with the traditional Chinese medicine compound composition group of the present invention was given intraperitoneal injection of cyclophosphamide at 30 mg/kg and 100 mg/kg by gavage. The tumor-bearing model group was given an equal volume of normal saline, once a day, for 16 consecutive days. 24h after the last administration, the mice were sacrificed by de-cervical vertebrae, the tumor tissue was stripped, weighed and the tumor inhibition rate was calculated: tumor weight inhibition rate (%)=(average tumor weight of control group-average tumor weight of treatment group)/average tumor weight of control group Weight × 100%.

1. Preparation of spleen lymphocytes

Mice were sacrificed by vertebral dissection, and their spleens were removed aseptically. The spleen was triturated with a glass slide, passed through a membrane, and centrifuged at 1200 rpm for 5 minutes. The supernatant was discarded, red blood cell lysate (about 1 ml per spleen) was added to the pellet, mixed well, allowed to stand for 1 minute, added with PBS, and centrifuged. Then add PBS, pass through membrane, and centrifuge. After washing the remaining cells 1-2 times, the cells were adjusted to the desired concentration with RPMI-1640 containing 10% FBS.

2. Detection of spleen lymphocyte proliferation function

The spleen lymphocytes in each group were inoculated into 96-well plates (4×10 ⁵ cells/well), and 2.5 μg/ml ConA was added for stimulation. The cells were cultured in a 5% CO2 incubator for 48 hours, and the splenic lymphocytes in each group were measured by MTT method. proliferation.

3. Enzyme-linked immunosorbent assay to detect the level of IL-2 production

Spleen lymphocytes in each group were inoculated into 96-well plates (4×10 ⁵ cells/well), and 2.5 μg/ml ConA was added to stimulate them. The cells were cultured in a 5% CO2 incubator for 16 h, and the culture supernatant was collected. Adsorption method was used to detect the cytokine production level in the culture supernatant.

2. Test results

1. Influence of the traditional Chinese medicine compound composition of the present invention on the lymphocyte spleen weight of H22 tumor-bearing mice

The results in Table 1 show that after 16 days of drug intervention, the spleen was isolated and weighed; the results showed that, compared with the mice in the tumor-bearing model group, the spleen of the chemotherapy drug cyclophosphamide group was significantly lower than that of the mice in the tumor-bearing model group, and the difference was significant. Compared with the chemotherapeutic drug cyclophosphamide group, the traditional Chinese medicine compound composition decoction drug intervention of the present invention significantly increased the spleen weight of the mice in the cyclophosphamide group, suggesting that the traditional Chinese medicine compound composition of the present invention has a certain effect on immune organs.

n＝8)Table 1. the influence of invention Chinese medicine composition decoction on lymphocyte spleen weight of H22 tumor-bearing mice (

n=8)

Compared with the tumor-bearing model group: ^* P﹤0.05, ^** P﹤0.01; compared with the cyclophosphamide group ^# P﹤0.05, ^## P﹤0.01

2. Influence of the traditional Chinese medicine compound composition of the present invention on the proliferation function of lymphocytes in H22 tumor-bearing mice

The results in Table 2 show that after 16 days of drug intervention, spleen lymphocytes were isolated from each group, and T lymphocytes were cloned under the induction of ConA, thereby inducing T lymphocyte proliferation; MTT test results showed that compared with the cyclophosphamide group, this The OD value of the traditional Chinese medicine compound composition intervention group or the combined drug group of the present invention was significantly higher than that of the tumor-bearing model group, and the differences were all significant (P﹤0.01). The OD value of the medication group was significantly increased, and the difference was significant (P﹤0.01).

n＝8)Table 2. the effect of traditional Chinese medicine compound composition group of the present invention on lymphocyte proliferation function of H22 tumor-bearing mice (

n=8)

group Dosage (mg/kg) OD value Tumor-bearing model group — 0.36±0.03 Traditional Chinese medicine compound composition group of the present invention 100mg/kg 0.34±0.07 Cyclophosphamide group 30mg/kg 0.26±0.10 Combination group 100mg/kg+30mg/kg 0.51±0.02^**##

Compared with the tumor-bearing model group: ^* P﹤0.05, ^** P﹤0.01; compared with the cyclophosphamide group ^# P﹤0.05, ^## P﹤0.01

3. The effect of the traditional Chinese medicine compound composition of the present invention on the production of IL-2 in lymphocytes of H22 tumor-bearing mice

The results in Table 3 show that, compared with the tumor-bearing model group of mice, cyclophosphamide drug intervention significantly inhibited the level of IL-2 production in spleen lymphocytes; The level of IL-2 production in spleen lymphocytes was significantly higher than that in the control group.

n＝8)Table 3. the effect of Chinese medicine compound composition of the present invention on H22 tumor-bearing mouse spleen lymphocyte IL-2 production level (

n=8)

Compared with the tumor-bearing model group: ^* P﹤0.05, ^** P﹤0.01; compared with the cyclophosphamide group ^# P﹤0.05, ^## P﹤0.01

The above results show that the traditional Chinese medicine compound composition of the present invention can significantly improve the proliferation of spleen lymphocytes and the level of IL-2 secretion, thereby enhancing the tumor immunity of tumor-bearing mice.

The beneficial effects of the present invention are as follows: in the traditional Chinese medicine compound composition provided by the present invention, astragalus and ginseng are the monarch medicine, tonifying qi and raising yang, and strengthening the pulse; As a ministerial medicine, Banzhilian assists Astragalus and Ginseng to improve human immunity, and can also contain Astragalus, warm and anti-tumor. The above-mentioned six herbs complement each other, and have the effects of strengthening the body and eliminating pathogens, and tonic for deficiency; modern pharmacology shows that the traditional Chinese medicine compound composition of the present invention can increase the weight of spleen lymphocytes, improve the proliferation of spleen lymphocytes and the level of IL-2 secretion, thereby enhancing tumor patients. The effect of immunity, prolong the survival period of tumor patients and improve the quality of life.

Detailed ways

The present invention is further described below by way of examples, but the present invention is not limited to the scope of the described examples.

Example 1

A traditional Chinese medicine compound composition with the function of regulating immunity is composed of the following raw materials by weight: astragalus 30g, ginseng 10g, ganoderma lucidum 10g, bark lotus 15g, atractylodes 15g, licorice 10g.

Add 30g of Astragalus, 10g of ginseng, 10g of Ganoderma lucidum, 15g of Banzhilian, 15g of Atractylodes Rhizoma, and 10g of Licorice with water and decocted and extracted 3 times, 50min each time, add 10 times the amount of water for the first time, add 8 times the amount of water for the second time, and then add 8 times the amount of water for the second time. Add 6 times the amount of water three times to obtain a mixed solution; filter the mixed solution through a 500-mesh sieve, then sterilize and bottle at high temperature to prepare a decoction with an immune-regulating function.

Add 30g of Astragalus, 10g of ginseng, 10g of Ganoderma lucidum, 15g of Banzhilian, 15g of Atractylodes Rhizoma, and 10g of Licorice with water and decocted and extracted 3 times, 50min each time, add 10 times the amount of water for the first time, add 8 times the amount of water for the second time, and then add 8 times the amount of water for the second time. Add 6 times the amount of water three times, filter the extract through a 500-mesh sieve, combine the filtrates, and concentrate to an extract with a relative density of 1.32; add 20g sucrose powder and 72g dextrin to the extract, mix well, and add 75% ethanol 60ml is granulated and dried at 63°C to make granules, tablets or pills.

Example 2

A traditional Chinese medicine compound composition with immune regulation function is composed of the following raw materials by weight: 25 g of astragalus, 5 g of ginseng, 5 g of ganoderma lucidum, 10 g of bark lotus, 10 g of atractylodes, and 15 g of licorice.

Add 25g of Astragalus, 5g of ginseng, 5g of Ganoderma lucidum, 10g of Banzhilian, 10g of Atractylodes Rhizoma, and 15g of Licorice in water to extract 3 times, each 40min, add 8 times the amount of water for the first time, add 6 times the amount of water for the second time, Add 4 times the amount of water three times to obtain a mixed solution; filter the mixed solution through a 520-mesh sieve, then sterilize and bottle at high temperature to prepare a decoction with an immune-regulating function.

Add 25g of Astragalus, 5g of ginseng, 5g of Ganoderma lucidum, 10g of Banzhilian, 10g of Atractylodes Rhizoma, and 10g of Licorice in water to extract 3 times, 40min each time, add 8 times the amount of water for the first time, add 6 times the amount of water for the second time, Add 4 times the amount of water three times, filter the extract through a 500-mesh sieve, combine the filtrates, and concentrate to an extract with a relative density of 1.32; add 15g sucrose powder and 70g dextrin to the extract, mix well, and add 75% ethanol 40ml is granulated and dried at 60°C to make granules, tablets or pills.

Example 3

A traditional Chinese medicine compound composition with regulating immune function, which is composed of the following raw materials by weight:

Astragalus 35g, Ginseng 15g, Ganoderma lucidum 15g, Banzhilian 20g, Atractylodes 20g, Licorice 20g.

Add 35g of Astragalus, 15g of ginseng, 15g of Ganoderma lucidum, 20g of Banzhilian, 20g of Atractylodes Rhizoma, and 20g of Licorice in water for 3 times, 60min each time, add 12 times the amount of water for the first time, add 10 times the amount of water for the second time, Add 8 times the amount of water three times to obtain a mixed solution; filter the mixed solution through a 550-mesh sieve, then sterilize and bottle at high temperature to prepare a decoction with an immune-regulating function.

Add 35g of Astragalus, 15g of ginseng, 15g of Ganoderma lucidum, 20g of Banzhilian, 20g of Atractylodes Rhizoma, and 20g of Licorice in water to extract 3 times, each time for 60min, add 12 times the amount of water for the first time, add 10 times the amount of water for the second time, Add 8 times the amount of water three times, filter the extract through a 550-mesh sieve, combine the filtrates, and concentrate to an extract with a relative density of 1.35; add 25g sucrose powder and 75g dextrin to the extract, mix well, and add 75% ethanol 100ml is granulated and dried at 65°C to make granules, tablets or pills.

Claims (7)

1. A traditional Chinese medicine compound composition with the function of regulating immunity is characterized by comprising the following components by weight

The raw materials in proportion are as follows: 25-35g of astragalus membranaceus, 5-15g of ginseng, 5-15g of lucid ganoderma, 10-20g of sculellaria barbata, 10-20g of bighead atractylodes rhizome and 10-20g of liquorice.

2. The compound traditional Chinese medicine composition for regulating immune function according to claim 1, characterized in that

The feed is characterized by comprising the following raw materials in parts by weight: 30g of astragalus, 10g of ginseng, 10g of lucid ganoderma, 15g of barbed skullcap herb, 15g of largehead atractylodes rhizome and 10g of liquorice.

3. The traditional Chinese medicine compound composition for regulating the immune function according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 25g of astragalus, 5g of ginseng, 5g of lucid ganoderma, 10g of sculellaria barbata, 10g of bighead atractylodes rhizome and 15g of liquorice.

4. The application of the compound traditional Chinese medicine composition for regulating the immune function in preparing the anti-tumor traditional Chinese medicine preparation according to the claims 1 to 3 is characterized in that the traditional Chinese medicine preparation is decoction, granules, tablets or pills.

5. The application of the traditional Chinese medicine compound composition for regulating the immune function in preparing an anti-tumor traditional Chinese medicine preparation according to claim 4 is characterized in that the preparation method of the decoction is as follows:

1) decocting radix astragali, Ginseng radix, Ganoderma, herba Scutellariae Barbatae, Atractylodis rhizoma, and Glycyrrhrizae radix in water for 3 times, 1 hr each time, adding 8-12 times of water for the first time, 6-10 times of water for the second time, and 4-8 times of water for the third time to obtain mixed solution;

2) filtering the mixed solution through a 500-550-mesh sieve, sterilizing at high temperature and bottling to obtain the target product.

6. The application of the traditional Chinese medicine compound composition for regulating the immune function in preparing an anti-tumor traditional Chinese medicine preparation according to claim 4 is characterized in that the preparation method of the granules, the tablets or the pills is as follows:

s1, adding water into the astragalus, the ginseng, the ganoderma lucidum, the barbed skullcap herb, the largehead atractylodes rhizome and the liquorice according to the proportion, decocting and extracting for 3 times, wherein 1 hour is used for each time, 8-12 times of water is added for the first time, 6-10 times of water is added for the second time, 4-8 times of water is added for the third time, filtering extracting solutions, combining filtrates, and concentrating to obtain an extract with the relative density of 1.30-1.35;

s2, adding auxiliary materials into the extract, mixing uniformly, adding 0-100 ml of 75% ethanol, granulating, drying at 60-65 ℃, and preparing into granules, tablets or pills.

7. The application of the traditional Chinese medicine compound composition for regulating the immune function in preparing an anti-tumor traditional Chinese medicine preparation according to claim 5 is characterized in that the auxiliary materials are the following substances in parts by weight: 15-25g of sucrose powder and 70-75 g of dextrin.