CN111528221A - Nematicide taking turpentine as carrier and application thereof - Google Patents

Abstract

The invention discloses a nematicide taking turpentine as a carrier and application thereof, and relates to a nematicide taking turpentine as a carrier and application thereof. The invention aims to solve the problems that most of solvents used for dissolving pesticides are organic solvents which are difficult to degrade and are toxic to plants and human health, and the existing nematocides cannot be well dissolved with turpentine and then cannot be transported and conducted in pine bodies sufficiently so that the pesticide effect cannot be exerted sufficiently. The nematicide with turpentine as a carrier is applied to the prevention and treatment of plant pathogenic nematodes. Not only has no toxic action on the pine tree, but also solves the problem that the pesticide is difficult to be fully dissolved, transported and dispersed in the rosin on the premise of reducing pollution, ensures that the pesticide is fully dissolved and the pesticide effect is fully exerted. The invention is applied to the field of plant disease control.

Description

A kind of nematicide with turpentine as carrier and its application

technical field

The invention relates to a nematicide with turpentine as a carrier and its application.

Background technique

Plant pathogenic nematodes have caused serious damage to crops and forest resources in my country. Such as root knot nematode (Meloidogyne spp.), cyst nematode (Heterodera spp.), rice stem tip nematode (Aphelenchoidesbesseyi), etc., lead to crop yield reduction, serious harm and extremely difficult to control. In forestry, pine wiltdisease (Pine wiltdisease) is a devastating disaster caused by the pine wood nematode (Bursaphelenchus xylophilus) that infects pine trees. The pine wood nematode has been listed as a key quarantine object at home and abroad. Once the disease occurs, it will be difficult to contain. At present, it has a tendency to expand its living range and gradually move northward, causing huge damage to my country's pine forest resources, resulting in serious economic losses and damage. immeasurable consequential loss.

At present, chemical control is still an effective measure to control plant pathogenic nematodes, but the safety, environmental and health problems caused by the use of pesticides cannot be ignored. We must try our best to reduce the environmental pollution caused by chemical control. At present, most of the solvents used for dissolving pesticides are organic solvents. Such organic solvents are not only toxic to plants and human health, but also have long-term safety hazards because they are difficult to be degraded.

Although relatively safe aqueous preparations have been developed, the advantages of emulsifiable concentrates, such as stable preparation and good efficacy, cannot be ignored. In addition, in the process of pine wood nematode control, many nematicides are often diluted with water as a solvent when preparing a working solution, so the chemicals cannot be well dissolved with pine resin, and then cannot be fully transported and transported in the pine tree. conduct. The drug cannot fully contact the pine wood nematodes parasitic in the pine resin canals, so it cannot fully exert its efficacy, and we cannot control the development of the disease in the forest. Therefore, the development of green substitutes that are compatible with turpentine and light aromatic hydrocarbon solvents can further solve the problem, so as to better prevent and control agricultural and forestry plant pathogenic nematodes represented by pine wood nematodes.

SUMMARY OF THE INVENTION

The purpose of the present invention is to solve the problem that most of the solvents used for dissolving pesticides are organic solvents, which are difficult to degrade, poison plants and human health, and the existing nemicide liquid can not be well dissolved with turpentine, and then can not fully dissolve The transport and conduction in the pine tree cannot fully exert the medicinal effect. A nematicide with turpentine as the carrier and its application are proposed.

The turpentine as carrier of the present invention is composed of 0.1-85% nematicide, 0.1-20% cosolvent, 0.1-35% emulsifier and the balance of turpentine according to mass fraction.

The nematicide with turpentine as a carrier of the present invention is applied to the control of plant pathogenic nematodes.

Turpentine can be used as a solvent to stabilize the efficacy of the drug, and it can play a very good role in stabilizing the drug in terms of anti-ultraviolet, anti-strong sunlight, cold and hot storage of the preparation. In addition, turpentine is a terpene compound, which is related to the resistance of pine trees. It not only has repellent effect, but also has good fat solubility. The high content of α-pinene and β-pinene in turpentine is more helpful for pine trees to resist pine wood nematode infestation. According to the parasitic habit of different pathogenic nematodes, different contents of turpentine oil solution (0.1-99.9%) are selected to dilute the original drug in the application process of the nematicide using turpentine oil as a carrier. This method can solve the problem that the nematicide liquid is difficult to dissolve with the rosin, and ensure that the nematicide is fully transported and dispersed in the pine tree. Therefore, the application of the nematicide with turpentine as the carrier not only has no toxic effect on the pine tree itself, but also solves the problem that pesticides are difficult to fully dissolve, transport and disperse in turpentine under the premise of reducing pollution, and ensure that the chemicals are fully dissolved. The effect of the drug is fully exerted.

Description of drawings

Fig. 1 is the ratio of the number of diseased trees in the treatment group and the control group in Example 1.

Detailed ways

Embodiment 1: In this embodiment, a nematicide with turpentine as a carrier quality is composed of 0.1-85% nematicide, 0.1-20% cosolvent, 0.1-35% emulsifier and the balance of turpentine.

The sum of the components in this embodiment is 100%.

Turpentine can be used as a solvent to stabilize the efficacy of the drug, and it can play a very good role in stabilizing the drug in terms of anti-ultraviolet, anti-strong sunlight, cold and hot storage of the preparation. In addition, turpentine is a terpene compound, which is related to the resistance of pine trees. It not only has repellent effect, but also has good fat solubility. The high content of alpha pinene and beta pinene in turpentine is more helpful for pine trees to resist pine wood nematodes.

The application of the nematicide with turpentine as a carrier in this embodiment not only has no toxic effect on the pine tree itself, but also solves the problem that pesticides are difficult to fully dissolve, transport and disperse in turpentine under the premise of reducing pollution, and ensures that the chemicals are fully dissolved. , the drug effect is fully exerted.

Embodiment 2: The difference between this embodiment and Embodiment 1 is that the nematicide is aldicarb, carbofuran, furancarb, salicarb, kexianbao, kexiandan, omethoate, probromide Phosphorus, chlorfenaphos, fenamiphos, chlorfenaphos, thiazophos, thiazophos, phoxim, fensophos, methyl isofosphos, fenox, chlorpyrifos, cyclophosphine, Matrine, Lime Nitrogen, 1,3-Dichloropropene, Butulfacarb, Verbaum, Dianron, Chloropicrin, Milore, Methyl Bromide, 1,2-Di-3-Chlorobromopropane, 1, 2-dibromoethane, imidacloprid, isofosulfos-methyl, diarnon, ammonium phosphine, terbuthion, emamectin, cyfluranil, pyrimidinox, thiamethoxam, acitretin A mixture of one or more of the bacteriocin and methylaminoabamectin benzoate in any ratio. Others are the same as the first embodiment.

Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the emulsifier is ammonium salt of dodecylbenzenesulfonate, sodium lignosulfonate, alkylaryl polyoxypropylene polyoxyethylene ether, Phenethylphenol polyoxyethylene ether, tristyrylphenol polyoxyethylene polyoxypropylene ether, styrylphenol formaldehyde resin polyoxyethylene propylene block polyether, styrene phenol formaldehyde resin polyoxyethylene ether, octyl One or more of phenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, castor oil polyoxyethylene ether, tetradecanol polyoxyethylene ether, nonylphenol polyoxyethylene ether and propylene glycol block polyether Mixtures in any ratio. Others are the same as in the first or second embodiment.

Embodiment 4: The difference between this embodiment and Embodiments 1 to 3 is that the co-solvent is methanol, ethanol, glycerol, isoamyl alcohol, ethyl acetate, propyl acetate, butyl acetate, sec-butyl acetate One or more of ester, dimethyl carbonate, methyl salicylate, dimethyl formamide, dimethyl sulfoxide, Tween 80, acetone, N-ethylpyrrolidone, isophorone and xylene A mixture in any ratio. Others are the same as one of Embodiments 1 to 3.

Embodiment 5: The present embodiment is a nematicide with turpentine as a carrier applied to the control of plant pathogenic nematodes.

Embodiment 6: The difference between this embodiment and Embodiment 5 is: when using turpentine as a carrier nematicide to control plant pathogenic nematodes, the nematicide is diluted with turpentine with a mass concentration of 0.1-99.9%, and the dilution ratio is 500- 7000. Others are the same as the fifth embodiment.

Embodiment 7: The difference between this embodiment and Embodiment 5 or 6 is that the plant pathogenic nematodes are C. elegans, C. elegans, Root-knot nematode, Cyst Semi-piercing nematodes. Others are the same as one of the fifth or sixth embodiment.

Embodiment 8: The difference between this embodiment and any one of Embodiments 5 to 7 is that the nematode Umbellifera is a pine wood nematode or a pine wood nematode. Others are the same as one of Embodiments 5 to 7.

Embodiment 9: This embodiment is different from any one of Embodiments 5 to 8 in that: when preventing and controlling the nematode, the nematicide is diluted with turpentine with a mass concentration of 30-99.9%, and the dilution ratio is 500-7000. Others are the same as one of Embodiments 5 to 8.

The following experiments were carried out for verifying the beneficial effects of the present invention:

Example 1:

Add 1.8% abamectin, 1% dimethyl sulfoxide, 4% ammonium dodecylbenzenesulfonate, 3% castor oil polyoxyethylene ether in sequence according to mass fraction in the reaction kettle, and the rest are turpentine. At a speed of 5000 rpm, the mixture was uniformly mixed for 15 minutes to prepare a turpentine-based nematicide and 1.8% abamectin EC.

Comparative Example 1:

Add 1.8% abamectin, 1% dimethyl sulfoxide, 4% ammonium dodecylbenzenesulfonate, 3% castor oil polyoxyethylene ether in sequence by mass fraction in the reactor, and the rest are xylene, At a speed of 5000 rpm, the mixture was homogenized for 15 minutes to prepare 1.8% abamectin EC.

Comparative Example 2:

Commercially available 1.8% Abamectin EC was selected as Comparative Example 2 of this test.

(1) Comparison of solubility

The premise of using turpentine as a carrier to prepare a nematicide is that turpentine can replace light aromatic hydrocarbons to dissolve pesticides, so the solubility of turpentine and xylene to abamectin was determined, and the results are shown in Table 1. The solubility of turpentine is better than that of xylene.

pesticide name turpentine Xylene Avermectin 12 10

Table 1 Turpentine oil and xylene to compare the solubility of abamectin (g/L, 25 ℃)

(2) Stability test

Samples were sealed in ampoules with a syringe and weighed at room temperature. Place the sealed ampoules in a metal container, and then store the metal container in a thermostat at 54±2°C and -5±2°C for 14 days, and conduct thermal storage and low-temperature stability tests respectively. The results are shown in Table 2. After storage for 14d in high temperature and low temperature environment, the stability of three kinds of medicaments is qualified, but the abamectin decomposition rate is lower in embodiment 1, and the storage stability performance of embodiment 1 abamectin emulsifiable concentrate is illustrated to be better than comparative example 1 , 2 Abamectin EC, which further indicates that Abamectin can exist more stably in turpentine.

Table 2 Stability test of three nematicides after 14 days

(3) Other performance comparison

The surface tension of the above three groups of nemicide was measured with a surface tensiometer, and each group was repeated three times. The measurement results are shown in Table 3. The surface tension of Example 1 is lower than that of Comparative Examples 1 and 2, and the lower surface tension helps the nematicide to spread and cover within the pine tree. In addition, the penetration time of Example 1 is significantly shorter than that of Comparative Examples 1 and 2, which is more favorable for the nematicide to exert its efficacy.

Performance comparison Example 1 Comparative Example 1 Comparative Example 2 Surface Tension 29.98 31.59 31.87 Penetration time 35.21s 48.36s 49.22s

Table 3 Performance comparison of three kinds of nemicide

Based on the above performance test results, it can be seen that turpentine, as an organic solvent, has a solubility greater than that of xylene, and can be used as a green preparation to replace light aromatic hydrocarbons. The nematicide using the turpentine oil of the present invention as a carrier reaches the quality standard of commercial pesticides, and is better than the two groups of controls in terms of stability, surface tension and penetration time.

(4) Nemicide treatment

The application of the nematicide described in this example takes the plant pathogenic nematode pine wood nematode as an example.

Since pine wood nematodes damage pine trees in the resin canal, whether the nematicide can fully dissolve with pine resin after entering the tree trunk is the key to affecting its control effect. In the application of the nematicide with turpentine as a carrier of the present invention, selecting a solution containing turpentine to dilute the original drug can further promote the dissolution of the nematicide and turpentine.

Example 1 was diluted with 99.9% turpentine oil solution, and Example 1 and Comparative Examples 1 and 2 were diluted with sterile water, and the dilution ratio was 2000 times. The control treatment of Example 1 was 99.9% turpentine oil solution, which was named as Control 1; The remaining controls were treated with sterile water. Controls were set up to exclude errors caused by other factors.

The pine tree tested in this experiment was Pinus sylvestris. Healthy pine sylvestris were selected as test pine trees. Measure the diameter at breast height of the pine tree to be treated at a distance of 1.5m from the ground, and apply the medicine at a dosage of 1.25ml per centimeter of diameter at breast height. 8 groups of sample plots of Pinus sylvestris were treated respectively with Example 1+turpentine oil, Example 1+sterile water, Comparative Example 1+sterile water, Comparative Example 2+sterile water and the corresponding control, with 100 plants in each group, Regularly observe the condition of the pine trees.

(5) Inoculation with pine wood nematodes

After 3 months, the pine wood nematodes were inoculated, and the nematode suspension was injected at a concentration of 10,000/plant, and the condition of the pine trees was observed regularly.

(6) Statistics on prevention and control effects

After 3 months, 8 groups of Pine sylvestris were investigated, the number of diseased trees was counted and recorded, and the ratio of the number of diseased trees in the treatment group and the control group was calculated. The specific situation is shown in Figure 1. As can be seen from Figure 1, the ratio of the number of diseased trees of Example 1+turpentine oil is significantly lower than that of Example 1+sterile water, Comparative Example 1+sterile water, Comparative Example 2+sterile water, the control effect of four groups of nematicides. 95%, 90%, 85% and 84% can be achieved, respectively. It can be seen that the turpentine oil solution is used for dilution, that is, the nematicide with turpentine oil as the carrier of the present invention has the best control effect.

The following conclusions can be drawn from the results of this test: First, turpentine as an organic substance has the value of formulation development and can dissolve the original drug of pesticides. Secondly, the stability performance is very important for the pesticide formulation, which can ensure that it remains active during long-distance transportation and storage, and the stability performance of Example 1 is better than that of Comparative Examples 1 and 2. More importantly, the lower surface tension and faster penetration rate allow the nematicide to disperse and spread more rapidly in the pine tree, which has been demonstrated in the application of this example to control pine wood nematodes. The statistical results of the prevention and control effect show that the control effect of the Example 1+turpentine oil group is better than that of the Example 1+sterile water, the comparative example 1+sterile water, and the comparative example 2+sterile water, which further proves that the turpentine oil is used as the carrier to prepare The nematicide is of great significance for the control of pine wood nematodes. The pine wood nematodes enter the pine tree when they feed on the pine tree, and multiply in the resin canal. Using turpentine as a carrier can not only ensure the full transportation and dispersion of the drug in the pine tree, but also promote the full dissolution of the nematicide and the resin due to its characteristics, and then fully contact the pathogen pine wood nematode. Using turpentine as a carrier further fundamentally improves the control effect on pine wood nematodes on the basis of the original excellent performance of the nematicide of the present invention.

In addition, turpentine is a natural product derived from nature, which not only has good environmental ecological safety, but also has no toxic effect on the applied plant itself as a plant extract. If the present invention can be put into pesticide production, it will not only help to improve the pollution situation and promote the development of agriculture and forestry, but also better exert the medicinal effect and strictly control the occurrence and development of plant pathogenic nematodes.

Claims (9)

1. a turpentine oil is the nematicide of carrier, it is characterized in that this nematicide is made up of the turpentine oil of 0.1-85% nematicide, 0.1-20% cosolvent, 0.1-35% emulsifier and surplus by mass fraction . 2. a kind of turpentine oil according to claim 1 is the nematicide of carrier, it is characterized in that the nematicide is aldicarb, carbofuran, furancarb, salicylic acid, Kelinebao, Kelinedan, oxygen Dimethoate, profenofos, grammephos, fenamiphos, chlorfenaphos, chlorazophos, thiazophos, thiazophos, phoxim, phoxofos, isofenfos-methyl, chlorfenaphos, chlorpyrifos, Butylphosphine, Matrine, Lime Nitrogen, 1,3-Dichloropropene, Butulfacarb, Verbamu, Dianron, Chloropicrin, Milore, Methyl Bromide, 1,2-Di-3-Chloro Bromopropane, 1,2-Dibromoethane, Imidacloprid, Methyl isofosphos, Diaron, Amphos, Terbuthion, Emamectin, Cyanfluranil, Pyridoxine, Fluthiam A mixture of one or more of sulfone, abamectin and methylamino abamectin benzoate in any ratio. 3. a kind of turpentine oil according to claim 1 is the nemicide of carrier, it is characterized in that emulsifying agent is dodecylbenzene sulfonic acid ammonium salt, sodium lignosulfonate, alkyl aryl polyoxypropylene polyoxygen Vinyl ether, phenethylphenol polyoxyethylene ether, tristyrylphenol polyoxyethylene polyoxypropylene ether, styrylphenol formaldehyde resin polyoxyethylene propylene block polyether, styrene phenol formaldehyde resin polyoxyethylene ether , one of octylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, castor oil polyoxyethylene ether, tetradecanol polyoxyethylene ether, nonylphenol polyoxyethylene ether and propylene glycol block polyether or multiple mixtures in any ratio. 4. a kind of turpentine oil according to claim 1 is the nematicide of carrier, it is characterized in that cosolvent is methyl alcohol, ethanol, glycerol, isoamyl alcohol, ethyl acetate, propyl acetate, butyl acetate, acetic acid One of sec-butyl ester, dimethyl carbonate, methyl salicylate, dimethyl formamide, dimethyl sulfoxide, Tween 80, acetone, N-ethylpyrrolidone, isophorone and xylene or multiple mixtures in any ratio. 5. turpentine oil as claimed in claim 1 is the application of the nematicide of carrier, it is characterized in that the nematicide of turpentine oil as carrier is applied to the control of plant pathogenic nematodes. 6. a kind of turpentine according to claim 5 is the application of the nematicide of carrier, it is characterized in that when using turpentine as the nematicide of carrier to control phytopathogenic nematodes, adopt the turpentine dilution of mass concentration 0.1-99.9% to kill nematicides agent, the dilution ratio is 500-7000. 7. a kind of turpentine oil according to claim 5 is the application of the nematicide of carrier, it is characterized in that the phytopathogenic nematode is the nematode Umbellifera, the nematode, the root knot nematode, the cyst nematode, the perforating nematode, the stem nematode , short-bodied nematodes or semi-piercing nematodes. 8. a kind of turpentine oil according to claim 5 is the application of the nematicide of carrier, it is characterized in that Umbellifera is pine wood nematode or pine wood nematode. 9. a kind of turpentine according to claim 7 or 8 is the application of the nematicide of carrier, when it is characterized in that preventing and controlling the nematode, adopting mass concentration is the turpentine dilution nematicide of 30-99.9%, dilution ratio 500-7000.

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