CN111458529B - Blood coagulation routine test constant speed test method - Google Patents
Blood coagulation routine test constant speed test method Download PDFInfo
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- CN111458529B CN111458529B CN202010446032.XA CN202010446032A CN111458529B CN 111458529 B CN111458529 B CN 111458529B CN 202010446032 A CN202010446032 A CN 202010446032A CN 111458529 B CN111458529 B CN 111458529B
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- 238000010998 test method Methods 0.000 title claims abstract description 17
- 238000009666 routine test Methods 0.000 title claims abstract description 12
- 230000023555 blood coagulation Effects 0.000 title claims abstract description 11
- 238000012360 testing method Methods 0.000 claims abstract description 175
- 238000001514 detection method Methods 0.000 claims abstract description 110
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 37
- 239000008280 blood Substances 0.000 claims abstract description 29
- 210000004369 blood Anatomy 0.000 claims abstract description 29
- 238000004519 manufacturing process Methods 0.000 claims abstract description 11
- 238000004458 analytical method Methods 0.000 claims abstract description 10
- 230000005540 biological transmission Effects 0.000 claims abstract description 10
- 238000010438 heat treatment Methods 0.000 claims abstract description 7
- 238000004140 cleaning Methods 0.000 claims description 9
- 238000009534 blood test Methods 0.000 claims description 2
- 230000015271 coagulation Effects 0.000 claims 6
- 238000005345 coagulation Methods 0.000 claims 6
- 238000000034 method Methods 0.000 description 7
- 238000005070 sampling Methods 0.000 description 6
- 230000023597 hemostasis Effects 0.000 description 3
- 230000000740 bleeding effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000002439 hemostatic effect Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 101100327917 Caenorhabditis elegans chup-1 gene Proteins 0.000 description 1
- 206010053567 Coagulopathies Diseases 0.000 description 1
- 208000028399 Critical Illness Diseases 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000035602 clotting Effects 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000035931 haemagglutination Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 238000004879 turbidimetry Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
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Abstract
The invention discloses a blood coagulation routine test constant speed test method, which is characterized in that each test cup is provided with a set of photoelectric signal detection and analysis and data transmission device and a constant temperature device, each test cup carries out the detection of one item, a plurality of test cups are placed on a live production line, and the live production line drives the test cups to move and supplies power to the test cups; the electrified assembly line drives the test cups to move for a certain distance at fixed time intervals, and each movement enables the next test cup to reach the position of the previous test cup when the test cup is paused; when the test cup is paused, the detection instrument adds a blood sample into the test cup at a fixed position, then a constant temperature device of the test cup is started, and the blood sample in the test cup is preheated for 120 s; after 120s of pre-heating is finished, adding a detection reagent into the test cup by the detection instrument at a fixed position, and starting to detect the blood sample by the test cup and uploading a detection result to the instrument; and after the detection is finished, the detection instrument cleans the test cup at a fixed position.
Description
Technical Field
The invention belongs to the field of medicine testing equipment, and particularly relates to a blood coagulation routine testing method.
Background
Before a patient is hospitalized and operated, a doctor always requires the patient to take blood for routine blood coagulation examination, and the aim is to know whether the hemostasis function of the patient is flawless before the operation so as to prepare in advance and prevent large bleeding in the operation from being too late. The hemostatic function of the human body is very important. When people accidentally injure and bleed, the hemostatic function quickly plays a role, so that blood is coagulated to plug wounds to stop bleeding, and the blood is prevented from being lost in large quantity. When a patient needs an operation, a doctor must know the hemostasis function of the patient in advance, for example, the hemostasis function is not perfect, and the patient may bleed greatly during the operation, so that an operation accident occurs and even the patient dies.
The routine examination of blood coagulation generally has six indexes, namely three qualitative indexes and three quantitative indexes.
From the aspect of detection time, three qualitative indexes can be completed within less than 1min generally, and three quantitative indexes are respectively 3min (FIB) and 8min (DD and FDP) according to clinical experience; the blood coagulation analyzer for routine blood coagulation examination which is used in large quantity at present realizes automatic sampling, automatic reagent adding and automatic test cup replacement, and in order to improve the detection efficiency, the test cup is made into an eight-linked cup form, the detection adopts a photoelectric turbidimetry method, each cup hole can be used for one test, the detection between cups is mutually independent, the detection can be carried out by the same index, and the detection can also be carried out by different indexes. When each detection is started, the test cup is kept static, a sample and a reagent are respectively added into the test cup by a sample adding needle capable of translating in XYZ three axes according to the detection index requirements set by a program, and after all detection items in the test cup are finished, the test cup is automatically replaced, and the next sample is tested continuously.
Although the detection efficiency of the eight-joint cup is greatly improved compared with that of a single cup, indexes of each patient to be detected are possibly different due to diversity and difference of pathogens of the patient, and some two, some four and still six complete detections are performed; at this time, if the detection simultaneously performed in the eight-linked cup has both quantitative indexes, the final detection completion time of the test cup is determined by the long-time quantitative index, and even if the quantitative index detection is completed, the next sample cannot be detected continuously, and the detection of the next sample can be performed only after the quantitative index detection is completed. Therefore, the efficiency of the test is not improved but is inhibited for the quantitative index detection in the current test method.
Disclosure of Invention
In order to solve the technical problems, the invention is realized by the following technical scheme: a blood coagulation routine test constant speed test method, equip a set of photoelectric signal detection analysis and data transmission device and thermostatic apparatus for each test cup, each test cup carries on the detection of an item, place a plurality of test cups on the live assembly line, drive the test cup to move and supply power to the test cup by the live assembly line; the electrified assembly line drives the test cups to move for a certain distance at fixed time intervals, and each movement enables the next test cup to reach the position of the previous test cup when the next test cup is paused; when the test cup is paused, the detection instrument adds a blood sample into the test cup at a fixed position, then the test cup moves forward by a distance of one test cup, the constant temperature device of the test cup is started, and the blood sample in the test cup is preheated for 120 s; after 120s of pre-heating is finished and the test cup is paused, the detection instrument adds a detection reagent into the test cup at a fixed position, the test cup starts a photoelectric signal detection analysis and data transmission device, starts to detect a blood sample and uploads a detection result to the instrument; after the detection is finished and the test cup is paused, the detection instrument cleans the test cup at a fixed position.
Further, when only one detection reagent is needed for a detection item performed by the test cup, the detection reagent is added into the test cup and detection is started after 120s of preheating is finished; when two detection reagents are needed for a detection item performed by the test cup, a first detection reagent is added into the test cup after the preheating is completed within 120s, and a second reagent is added into the test cup after 180s and the detection is started.
Further, the time interval for the test cup to move was 5 seconds.
Furthermore, the electrified assembly line is provided with a movable electric brush and a conductive bar, so that the power supply and the uploading of the detection result of the test cup are realized.
Furthermore, the electrified assembly line is in a closed ring shape, the position of the detecting instrument for cleaning the testing cup is adjacent to the position of adding the blood sample to the testing cup, and the electrified assembly line drives the testing cup to the position of adding the blood sample after the cleaning of the testing cup is finished.
Further, the product of the number of test cups and the running interval of the test cups is not less than the sum of the longest time consumption in the routine blood test items and the two moving intervals of cleaning the test cups and adding the blood samples.
Furthermore, a group of test cups is formed by a plurality of test cups, the test cups share the photoelectric signal detection and analysis device, the data transmission device and the constant temperature device to form a station unit, and the test cups in each station unit are independently detected.
Furthermore, when a sample needs to be tested for multiple items, the testing reagent of the item consuming the longest time is added into the testing cup first, so as to test the item consuming the longest time first, and the total time consumption of the multiple items of testing of a single sample is the shortest.
Furthermore, the test cups are closely arranged on the electrified assembly line, and the electrified assembly line drives the test cups to move by the distance of one test cup each time.
The invention has the advantages that the detection efficiency can be improved and the constant speed detection can be realized when a plurality of samples are detected.
Drawings
FIG. 1 is a schematic diagram of an embodiment of the present invention;
FIG. 2 is a flow chart of the time required to test two samples according to the present invention and the prior art method.
Detailed Description
The invention is described in detail below with reference to the following figures and embodiments:
a blood coagulation routine test constant speed test method, equip a set of photoelectric signal detection analysis and data transmission device and thermostatic apparatus for each test cup, each test cup carries on the detection of an item, place a plurality of test cups on the live assembly line, drive the test cup to move and supply power to the test cup by the live assembly line;
the electrified assembly line drives the test cups to move for a certain distance at fixed time intervals, and each movement enables the next test cup to reach the position of the previous test cup when the test cup is paused;
when the test cup is paused, the detection instrument adds a blood sample into the test cup at a fixed position, then the test cup moves forward by a distance of one test cup, the constant temperature device of the test cup is started, and the blood sample in the test cup is preheated for 120 s;
after 120s of pre-heating is finished and the test cup is paused, the detection instrument adds a detection reagent into the test cup at a fixed position, the test cup starts a photoelectric signal detection analysis and data transmission device, starts to detect a blood sample and uploads a detection result to the instrument;
and after the detection is finished and the test cup is paused, the detection instrument cleans the test cup at a fixed position.
The detecting instrument adds the blood sample to the testing cup through the sample adding unit component, adds the detection reagent to the testing cup through the reagent adding unit component, and cleans the testing cup through the cleaning unit component. The cleaning unit assembly can be referred to patent CN110624916A.
When only one detection reagent is needed for the detection items carried out by the test cup, the detection reagent is added into the test cup and the detection is started after the 120s pre-heating is finished; when two detection reagents are needed for a detection item performed by the test cup, a first detection reagent is added into the test cup after the preheating is completed within 120s, and a second reagent is added into the test cup after 180s and the detection is started. The first reagent is separated from the second reagent by 180 seconds, which time allows the first reagent to react sufficiently with the blood sample.
The time interval for the test cup to move was 5s. In the test method, only the test cup moves, and other components of the detection instrument do not need to move, so that respective actions can be completed in a short time, the time for moving the sample adding needle is saved, and the short time is generally within 5S. The time interval for the movement of the pipeline is now set to 5S, i.e. the test cup is moved once every 5S.
Electrified assembly line is equipped with portable brush and busbar to realize the power supply of test cup and upload the testing result. The structure can be referred to patent CN110568208A.
The electrified assembly line is closed annular, and the position that detecting instrument washd test cup is adjacent with the position that adds the blood sample to test cup, and test cup washs the position of accomplishing the back and being driven to adding the blood sample by electrified assembly line promptly. The closed annular electrified assembly line can realize the recycling of the test cup, so that the utilization rate of the test cup is high.
In the closed annular live-line production line, the product of the number of the test cups and the running intervals of the test cups is not less than the sum of the longest time consumption in the conventional blood detection item and the two moving intervals of cleaning the test cups and adding the blood samples, and the cyclic use of the test cups can be met only in this way. Usually, the longest detection time is 8min of immunoassay, and at this time, if the movement interval is 5s, the number of test cups needs to be 8 × 60 ÷ 5+2= 98. That is, the washing was performed immediately after the completion of the measurement within 8min after the addition of the blood sample, and a new measurement was performed immediately after the completion of the washing.
When one sample needs to be subjected to multiple detections, the detection reagent of the item consuming the longest time is added into the detection cup to detect the item consuming the longest time first, so that the total time consumption of the multiple detections of a single sample is shortest. If the FIB detection is 3min, and the DD and FDP detection is 8min, the DD and FDP detection is firstly carried out, and then the FIB detection is carried out, so that the total time of the multiple detections of one sample is 8min plus the time of adding the blood sample twice for 10s, and the total time is 8min10s.
When the device is actually used, a group of test cups can be formed by a plurality of test cups, the group of test cups share the photoelectric signal detection and analysis device, the data transmission device and the constant temperature device to form a station unit, and the test cups in each station unit are independently detected. In practice, the eight-in-one cup is often used.
The test cups can be arranged closely on the live production line, and the live production line drives the test cups to move by the distance of one test cup at each time, so that the production line is more compact and the efficiency is higher.
Taking the example that the test cups operate once at intervals of 5s and the electrified assembly line adopts a closed ring shape and the test cups are recycled, the test method can complete 720 tests every hour.
In contrast to existing test methods, it is assumed that the clotting routine of two samples, both from critically ill patients requiring urgent surgery, now needs to be tested. The indexes of the sample No. 1 needing to be detected are as follows: PT, APTT, TT, FIB; the indices for sample No. 2 are: PT, APTT, TT, FIB, DD, FDP.
Firstly, calculating the time required for completing two samples by using the existing full-automatic hemagglutination instrument, wherein the average sampling time of a sampling needle is 5S, the average reagent adding time of the sampling needle is 3S, and the time for replacing a reagent cup is 3S; referring to the left side of the flow chart in fig. 2, the time T =20+3+180+3+30 +480 + 480=719srequired for completing all the tests is calculated from the diagram. In the detection of each sample, the item which takes the longest time is performed, so that the time for adding the reagent is not accumulated, and only 3s is calculated once.
Then, according to the method, the test cup is moved forward at a speed of moving one frame every 5S, the sampling and reagent adding time is completed within 5S and can be simultaneously operated, and the time T =20+10+480=510S required for completing all tests is calculated as shown in the right side of the flow chart of FIG. 2. The time for adding the reagent into DD and FDP needs to be accumulated, and the time for detecting the two project items is the same.
It can be seen from above comparison that the efficiency of testing that has improved the sample after adopting the new test method of this patent greatly increases along with the quantity increase of actual clinical sample, efficiency promotion will be more obvious. And because the assembly line moves in steps at a continuous fixed time interval, the actions of re-sampling or adding reagent and the like can be carried out every moving step, and the following test can be carried out without waiting for the completion of long-time detection.
Furthermore, from the viewpoint of long-time continuous operation, the number of detections per hour in the existing method is not necessarily constant within a fixed time, and the longest time consumption of the test in each octal cup is changed according to different detection items; according to the method, the number of detections in each hour is constant and is irrelevant to detection items, high-speed and constant-speed detection can be realized as long as the stepping interval time of the assembly line is constant, and a hospital can make a detection plan and result reporting time according to the outpatient quantity and the patient quantity in a ward. In the prior instrument method, the detection time is related to the detection item, and the instrument cannot perform constant-speed detection, so that the clinical laboratory cannot accurately provide the report time of the detection result.
Fig. 1 shows an implementation structure of the method, wherein a test cup 1 is driven by a closed ring-shaped live production line 2 to circularly operate, and sequentially passes through a sample loading unit component 3, a first reagent loading unit component 41, a second reagent loading unit component 42 and a cleaning unit component 5.
It is to be emphasized that: the above embodiments are only preferred embodiments of the present invention, and are not intended to limit the present invention in any way, and all simple modifications, equivalent changes and modifications made to the above embodiments according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.
Claims (7)
1. A blood coagulation routine test constant speed test method is characterized in that: each test cup is provided with a set of photoelectric signal detection and analysis and data transmission device and a constant temperature device, each test cup carries out the detection of one item, a plurality of test cups are placed on a live production line, and the live production line drives the test cups to move and supplies power to the test cups; the electrified assembly line drives the test cups to move for a certain distance at fixed time intervals, and each movement enables the next test cup to reach the position of the previous test cup when the test cup is paused; when the test cup is paused, the detection instrument adds a blood sample into the test cup at a fixed position, then the test cup moves forward by the distance of one test cup, the constant temperature device of the test cup is started, and the blood sample in the test cup is preheated for 120 s; after 120s of preheating is finished, when the test cup is paused, the detection instrument adds a detection reagent into the test cup at a fixed position, the test cup starts a photoelectric signal detection and analysis and data transmission device, starts to detect a blood sample and uploads a detection result to the instrument; after the detection is finished and the test cup is paused, the test cup is cleaned by the detection instrument at a fixed position;
the product of the number of the test cups and the running interval of the test cups is not less than the sum of the longest time consumption in the routine blood test items and the two moving intervals of cleaning the test cups and adding the blood samples;
when a sample needs to be detected for multiple items, the detection reagent of the item which consumes the longest time is added into the test cup firstly, so that the item which consumes the longest time is detected firstly, and the total time consumption of multiple items of detection of a single sample is the shortest.
2. The coagulation routine test constant velocity test method according to claim 1, characterized in that: when only one detection reagent is needed for the detection items carried out by the test cup, the detection reagent is added into the test cup and the detection is started after the 120s pre-heating is finished; when two detection reagents are needed for a detection project carried out by the test cup, a first detection reagent is added into the test cup after 120s of pre-heating is finished, and a second reagent is added into the test cup after 180s of pre-heating and detection is started.
3. The coagulation routine test constant velocity test method according to claim 1, characterized in that: the time interval of the test cup moving is 5s.
4. The coagulation routine test constant velocity test method according to claim 1, characterized in that: the electrified assembly line is equipped with portable brush and busbar to realize the power supply of test cup and upload the testing result.
5. The coagulation routine test constant velocity test method according to claim 1, characterized in that: the electrified assembly line is in a closed ring shape, the position of the detection instrument for cleaning the test cup is adjacent to the position of the test cup for adding the blood sample, and the test cup is driven to the position for adding the blood sample by the electrified assembly line after being cleaned.
6. The coagulation routine test constant velocity test method according to claim 1, characterized in that: a group of test cups is formed by a plurality of test cups, the test cups share the photoelectric signal detection and analysis and data transmission device and the constant temperature device to form a station unit, and the test cups in each station unit are independently detected.
7. The coagulation routine test constant velocity test method according to claim 1, characterized in that: the test cups are closely arranged on the live production line, and the live production line drives the test cups to move by the distance of one test cup each time.
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| CN202010446032.XA CN111458529B (en) | 2020-05-25 | 2020-05-25 | Blood coagulation routine test constant speed test method |
| PCT/CN2020/130901 WO2021238109A1 (en) | 2020-05-25 | 2020-11-23 | Fixed-rate test method for blood coagulation routine test |
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| CN111458529B (en) * | 2020-05-25 | 2023-01-10 | 北京中勤世帝生物技术有限公司 | Blood coagulation routine test constant speed test method |
| CN112375670A (en) * | 2020-10-10 | 2021-02-19 | 四川大学 | Household convenient liquid microbial component detection device and detection method |
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| CN111458529A (en) | 2020-07-28 |
| WO2021238109A1 (en) | 2021-12-02 |
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