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CN111407695B - Sea-buckthorn water and preparation method and application thereof - Google Patents

Sea-buckthorn water and preparation method and application thereof Download PDF

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CN111407695B
CN111407695B CN202010227865.7A CN202010227865A CN111407695B CN 111407695 B CN111407695 B CN 111407695B CN 202010227865 A CN202010227865 A CN 202010227865A CN 111407695 B CN111407695 B CN 111407695B
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sea buckthorn
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seabuckthorn
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CN111407695A (en
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王春
赵荣
周文娜
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Shaanxi Haitian Pharmaceutical Co ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/007—Preparations for dry skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
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Abstract

The invention discloses sea-buckthorn water which is extracted from sea-buckthorn plants. The invention also discloses a preparation method of the sea buckthorn water, which is implemented according to the following steps: step 1, cleaning and pretreating sea buckthorn plants; and 2, extracting the raw materials obtained in the step 1 to obtain the sea buckthorn water. The fructus Hippophae water has effects of keeping moisture, preventing ultraviolet burn, repairing skin, whitening skin, resisting oxidation, and inhibiting skin melanin deposition. The research on sea buckthorn is mostly focused on the preparation and application of sea buckthorn oil. Compared with the seabuckthorn oil, the extraction and preparation of the seabuckthorn water are rarely researched, the seabuckthorn water is prepared by a supercritical carbon dioxide method, and the gap of preparation and research of the seabuckthorn water is filled.

Description

Sea-buckthorn water and preparation method and application thereof
Technical Field
The invention belongs to the field of cosmetics, and particularly relates to sea buckthorn water, and a preparation method and application of the sea buckthorn water.
Background
Sea buckthorn (Hippophae rhamnoides L.) is a plant of the genus Hippophae of the family Elaeagnaceae, is drought-resistant and sandstorm-resistant, and is an economic plant with multiple purposes in northwest plateau. The sea-buckthorn fruit is also a plant with homology of medicine and food, and has rich nutrient substances and bioactive substances in roots, stems, leaves, flowers and fruits, particularly the sea-buckthorn fruit has the effects of relieving cough and reducing sputum, invigorating stomach and promoting digestion, and activating blood circulation and dissipating blood stasis when being used as a medicine; the seabuckthorn seeds also contain various substances beneficial to human bodies, and the seabuckthorn seed oil extracted from the seabuckthorn seeds is applied to various fields. When the company extracts the seabuckthorn seed oil by supercritical carbon dioxide, a large amount of aqueous solution is separated from the extracted effective components by standing; after the solution is prepared into a mask, the mask is found to have moisturizing and other effects. The conventional sea buckthorn water is a part which is easy to neglect, and how to collect the sea buckthorn water in the sea buckthorn berries is particularly necessary.
To investigate other effects of the aqueous solution, the company studied it. Extracting the sea buckthorn water by adopting a proper method aiming at different parts of the sea buckthorn, and researching the biological activity of the sea buckthorn water.
Disclosure of Invention
The invention aims to provide the sea buckthorn water which has the effects of delaying senescence, brightening skin, moistening skin, inhibiting bacteria, diminishing inflammation and removing acnes and can effectively solve the problems of dry, dark, aged and acne marks of the skin.
The second purpose of the invention is to provide a preparation method of the sea buckthorn water.
The third purpose of the invention is to provide the application of the sea buckthorn water.
The first technical scheme adopted by the invention is as follows: a fructus Hippophae water is prepared by extracting fructus Hippophae.
The second technical scheme adopted by the invention is as follows: a preparation method of sea buckthorn water is specifically implemented according to the following steps:
step 1, cleaning and pretreating sea buckthorn plants;
and 2, extracting the raw materials obtained in the step 1 to obtain the sea buckthorn water.
The invention adopting the second technical proposal is also characterized in that,
the seabuckthorn plants in the step 1 comprise seabuckthorn seeds, dried seabuckthorn fruits, fresh seabuckthorn fruits, seabuckthorn leaves and seabuckthorn stems.
The pretreatment process of the sea buckthorn seeds or the dried sea buckthorn fruits in the step 1 is crushing and embryo pressing, the thickness of each piece is 0.3-0.5 mm, and the fine powder smaller than 18 meshes and smaller than 18 meshes after crushing does not exceed 10% of the total mass.
And 2, extracting the sea-buckthorn seeds/dried sea-buckthorn fruits by adopting supercritical carbon dioxide, extracting the fresh sea-buckthorn fruits by adopting a squeezing method, and extracting the sea-buckthorn leaves and the sea-buckthorn stems by adopting an azeotropic distillation method.
The invention adopts a third technical scheme that the invention is an application of sea buckthorn water in the field of cosmetics.
The invention has the beneficial effects that: the fructus Hippophae water has effects of keeping moisture, preventing ultraviolet burn, repairing skin, whitening skin, resisting oxidation, and inhibiting skin melanin deposition. The research on sea buckthorn mostly focuses on the preparation and application of sea buckthorn oil. Compared with the seabuckthorn oil, the extraction preparation method of the seabuckthorn water is rarely studied, the seabuckthorn water is prepared by a supercritical carbon dioxide method, and the gap of preparation research of the seabuckthorn water is filled.
Drawings
FIG. 1 is a flow chart of the preparation of sea buckthorn water according to the present invention;
FIG. 2 is a graph showing the effect of extraction temperature and extraction pressure on extraction rate according to the present invention;
FIG. 3 is a graph showing the effect of extraction pressure and extraction time on extraction yield according to the present invention;
FIG. 4 is a graph showing the effect of extraction time and carbon dioxide flow rate on extraction rate according to the present invention;
FIG. 5 is a comparison of the face of a patient before 1 week, after 3 weeks and after recovery from the application of sea buckthorn water of the present invention;
FIG. 6 is a graph of the effect of sea buckthorn water on the rate of apoptosis of UVC radiation HaCaT cells;
fig. 7 is a comparison of facial moisture content of the mask prepared using the present invention for the control group and the test group.
Detailed Description
The invention is further described with reference to the following figures and detailed description.
The invention relates to sea buckthorn water which is obtained by extracting and separating sea buckthorn plants. The method for preparing the sea buckthorn water from different parts of the sea buckthorn plant comprises the following steps:
the preparation method for extracting the sea buckthorn water from the sea buckthorn seeds or the dried sea buckthorn fruits is implemented according to the following steps:
step 1, taking the seabuckthorn seeds or the dried fruits, cleaning, crushing and tabletting, wherein the thickness of the tablets is 0.3-0.5 mm, and the crushed tablets are smaller than 18 meshes.
Step 2, pressing the raw materials obtained in the step 1CO at a pressure of 270-290 bar and a temperature of 52-55 deg.C 2 The flow rate is 2750 and 2850kg/h, and the extraction time is 4.5-5.5 h.
And 3, performing primary separation on the substance extracted in the step 2 under the conditions that the pressure is 100-110 bar and the temperature is 45-50 ℃, performing secondary separation under the conditions that the pressure is 50-55 bar and the temperature is 30-35 ℃, and standing and separating the oil-water substance obtained by separation under the conditions that the temperature is 40-45 ℃ and the time is 5 hours to obtain the sea buckthorn water.
(II) the preparation method of the sea-buckthorn water in the fresh sea-buckthorn fruits is implemented according to the following steps:
squeezing fresh fructus Hippophae to obtain juice, centrifuging the juice in a centrifuge at 3000r/min for more than 10min, standing to obtain upper layer, evaporating the upper layer solution at 45 deg.C, 40r/min and 0.1Mpa, and distilling to obtain water.
(III) the preparation method of the sea buckthorn water in the sea buckthorn leaves/sea buckthorn stems is implemented according to the following steps:
adding folium Hippophae and xylene at a mass ratio of 1:10 into an industrial distillation device, adjusting temperature at a heating rate of 2 deg.C/min, collecting the effluent when the temperature reaches azeotropic point of 92 deg.C, heating at constant temperature, and stopping heating when the temperature of azeotrope steam changes. Weighing the collected azeotrope, respectively placing the azeotrope in a liquid separating device, standing for layering, collecting a water sample after layering, weighing and calculating the water content of the water sample to obtain the sea buckthorn water,
as shown in table 1 below, the comparison of the amounts of water extracted from different parts of the sea buckthorn plant is performed, and the analysis of the amounts of water extracted from the dry sea buckthorn fruits, the sea buckthorn seeds, the fresh sea buckthorn fruits, the sea buckthorn leaves and the sea buckthorn stems extracted from the different parts of the plant sea buckthorn results in different amounts of water extracted from the different parts of the plant sea buckthorn, and the amount of water contained in the fresh sea buckthorn fruits is as large as 76-79%, which is caused by the fact that more water exists in the fresh sea buckthorn fruits.
TABLE 1 comparison table of water extraction amount of different parts of sea buckthorn
Dried sea buckthorn fruit (%) Sea buckthorn seed (%) Sea buckthorn fresh fruit (%) Sea buckthorn leaf (%) Sea buckthorn stem (%)
12～14.5 12～13 76～79 53～55 48～50
Comparison of sea buckthorn water and sea buckthorn oil: the sea buckthorn water obtained by the invention is a clear, transparent or semitransparent liquid with clear color, and the sea buckthorn oil is a transparent liquid with yellow to orange red. The oleum Hippophae and fructus Hippophae water both contain vitamins, flavone, carotene, linoleic acid, alpha-linolenic acid, and amino acids, but the oleum Hippophae contains less esters, fatty acids, and alcohols. The seabuckthorn oil is more applied to the aspect of medical treatment, while the seabuckthorn water is light in color and rich in amino acid, vitamins and other substances, and is clear in water quality, so that the seabuckthorn oil is more conveniently applied to the aspect of beauty and skin care.
Optimizing the extraction process of sea buckthorn water by taking the extraction of sea buckthorn seed oil as an example
The method comprises determining 4 main factors by integrating the influence of each factor on the extraction rate of the oil-water mixture of seabuckthorn seeds, adopting Box-Bohnken (BBD) combination Design in Design-Expert 8.0.6, and adopting A extraction temperature, B extraction pressure and C CO 2 The flow rate and the extraction time D are independent variables,the extraction rate of the seabuckthorn seed oil is taken as a dependent variable for analysis, and the factors and the coding level are shown in a table 2:
TABLE 2 analysis and leveling of response surface factors
Figure BDA0002428301750000051
TABLE 3 response surface test design and results
Figure BDA0002428301750000052
Figure BDA0002428301750000061
The structure of the response surface BBD test scheme is shown in Table 3, and after the test is performed according to the response surface BBD test scheme, the least square method is used for fitting a relational equation between the response value Y and the independent variable X as follows:
Y＝21.46+0.03X 1 +0.99X 2 +0.37X 3 +2.57X 4 +0.25X 1 X 2 +0.0025X 1 X 3 +1.32X 1 X 4 +0.0025X 2 X 3 +0.11X 2 X 4 +0.72X 3 X 4 +7.7X 1 2 +0.076X 2 2 +8.24X 3 2 +3.61X 4 2
the results of analysis of variance and significance test on the model equation show that the effect of extraction temperature, carbon dioxide flow and extraction time on the extraction of the seabuckthorn oil is more significant, and the interaction among all factors is more obvious. The regression significance of the model (P is less than 0.0001), the complex correlation coefficient of the model is 0.9935, and the correction decision coefficient Adj-R of the model 2 0.9695, the model predicts a change in the response value Y of 96.95%. Therefore, it is feasible that this model is used to optimize the extraction yield of the oil-water mixture of seabuckthorn. The optimal process conditions for extracting the seabuckthorn oil by the response surface optimization method are as follows: the extraction temperature was 53.7 deg.CThe extraction pressure is 281bar, CO 2 The flow rate is 2807kg/h, the extraction time is 5h, and the collection rate of the seabuckthorn oil-water mixture is 9.6%. Experiments verify that the response surface method can perform regression analysis and parameter optimization on the extraction of the seabuckthorn oil-water mixture.
(1) Influence of extraction temperature and extraction pressure on extraction rate
As shown in fig. 2, through the optimization of the response curved surface, under the condition of a certain extraction temperature, the increase of the extraction rate of the seabuckthorn oil-water mixture is not changed greatly along with the increase of the extraction pressure, and under the condition of a certain extraction pressure, the extraction rate is increased firstly and then reduced along with the increase of the extraction temperature. Through optimization, the optimal extraction temperature of the method is 57.3 ℃, and the extraction pressure is 281 bar.
(2) Influence of extraction pressure and extraction time on extraction rate
As shown in fig. 3, through the optimization of the response surface, under the condition of a certain extraction pressure, the extraction rate of the seabuckthorn oil-water mixture is gradually increased along with the increase of the extraction time, but the change of the extraction rate gradually decreases when the extraction rate is increased to a certain trend. Through optimization, the optimal extraction pressure is 281bar, and the extraction time is 5 h.
(3) Influence of extraction time and carbon dioxide flow on extraction rate
As shown in fig. 4, through the optimization of the response curved surface, under the condition of a certain extraction pressure, the growth rate of the seabuckthorn oil-water mixture is increased and then decreased along with the increase of the carbon dioxide flow. Under the condition of a certain carbon dioxide flow, the change range of the extraction rate of the sea buckthorn oil-water mixture is small along with the increase of the extraction pressure. Through optimization, the optimal extraction time of the invention is 5h, and the flow of carbon dioxide is 2807 kg/h.
The effective components and efficacy analysis and verification of the sea buckthorn water prepared by the invention are as follows:
the effective components of the medicinal composition are analyzed and researched, and the specific results are shown in table 4:
TABLE 4 effective components of Hippophae rhamnoides in water
Figure BDA0002428301750000081
Figure BDA0002428301750000091
The ratio of each active ingredient is shown in table 5:
TABLE 5 ratio of effective components of fructus Hippophae water
Figure BDA0002428301750000092
Figure BDA0002428301750000101
The effect of each component in the sea buckthorn water is as follows: leucine can reduce blood sugar concentration, promote the repair of bone, skin and muscle tissue, and is used as the supplementary product for postoperative rehabilitation. Isoleucine can reduce blood sugar concentration, promote the repair of bone, skin and muscle tissue, and is a supplement for postoperative rehabilitation. Valine can reduce blood sugar concentration, promote the repair of bone, skin and muscle tissue, and is a supplement for postoperative rehabilitation. Alanine (L-Alanine) plays an important role in the in vivo protein synthesis process, and during in vivo amino acid metabolism, ketoacid is produced after deamination, and sugar is produced according to a glucose metabolic pathway. The vitamin E can effectively reduce the generation of wrinkles, keep youthful appearance, resist oxidation, protect body cells from being poisoned by free radicals, resist aging and cancer, prevent inflammatory dermatosis and alopecia, protect skin from being damaged by ultraviolet rays and pollution, reduce the deposition of scars and pigments, accelerate the healing of wounds, prevent the coagulation of blood and reduce the generation of speckle tissues. Beta-carotene is an antioxidant, has detoxifying effect, is an indispensable nutrient for maintaining human health, has remarkable functions in resisting cancer, preventing cardiovascular diseases, cataract and oxidation, and further prevents various degenerative diseases caused by aging and senility. Linoleic acid is known as an essential fatty acid because it must be obtained from food because the human body cannot synthesize it or synthesizes it very little by itself. Because linoleic acid can reduce blood cholesterol and prevent atherosclerosis, and meanwhile, linoleic acid can prevent or reduce the incidence rate of cardiovascular and cerebrovascular diseases, the linoleic acid is particularly favorable for preventing and treating hypertension, hyperlipidemia, angina pectoris, coronary heart disease, atherosclerosis, senile obesity and the like, can prevent the deposition of serum cholesterol of a human body on the vessel wall, and has the health-care effect of preventing and treating atherosclerosis and cardiovascular and cerebrovascular diseases. The alpha-linolenic acid can enhance intelligence, improve memory, protect eyesight and improve sleep. Inhibiting thrombosis diseases, preventing myocardial infarction and cerebral infarction, reducing blood lipid, and lowering blood pressure. Inhibiting hemorrhagic apoplexy, and preventing allergy. The total flavone has effects of protecting hematopoietic system and immune system, scavenging free radicals, resisting oxidation, and protecting DNA. Malic acid has effects of relieving fatigue, protecting liver, kidney, and heart; can improve energy metabolism of brain tissue, regulate neurotransmitter in brain, and is beneficial to recovery of learning and memory function, and has obvious improving effect on learning and memory. Vitamin C has effects of decomposing melanin in skin, preventing pigmentation, preventing and treating chloasma and freckle, keeping skin white and tender, promoting wound healing, and strengthening blood vessel and skeleton. Meanwhile, the collagen biological synthesis is promoted; the tissue wound can be healed more quickly; promoting the metabolism of tyrosine and tryptophan in amino acid; the life of the human body is prolonged; improving the utilization of iron, calcium and folic acid; improving the metabolism of fats and lipids, particularly cholesterol; preventing cardiovascular diseases; promoting the growth of teeth and bones and preventing gum bleeding; enhancing the anti-stress capability and immunity of the body to the external environment.
The effect verification of the sea buckthorn water prepared by the invention is as follows:
(1) irritation property
The experimental process comprises the following steps: smearing 5mL of sea buckthorn water on one wrist, continuously smearing for 15 days while keeping smearing once a day, and observing by taking the other wrist as a control, wherein the experimental results are shown in Table 6. The test was divided into two parallel groups, each group being divided into a sample group and a control group.
TABLE 6 integral of skin irritation response to repeated contact with sea buckthorn
Figure BDA0002428301750000111
Figure BDA0002428301750000121
As can be seen from Table 6: through analysis and comparison, the skin smeared by the sample does not have the adverse phenomena of red spots, skin edema and the like compared with a control group. It follows that the sea buckthorn water sample is not irritating to the skin.
(2) Water replenishing effect
The experimental process comprises the following steps: the company randomly draws 50 people, wipes 5mL of the composition on the left wrist, and keeps using the composition once a day for one week. The other wrist was used as a control for observation, and the results are shown in Table 7.
TABLE 7 Water supplement results
Figure BDA0002428301750000122
And (4) conclusion: the skin of the test person becomes moist, tender and smooth after insisting on using the sea buckthorn water for one month, and the roughness of the skin of the test person is obviously relieved. The ratio of good, ordinary and poor skin moisturizing performance after the product is considered by the test person is respectively as follows: 96%, 3% and 1%.
(3) Has effects in removing acne marks on skin.
Experiments randomly select face skin with acne marks at the age of 24-30 years, and the selection of the subjects meets the test standards of the experiments. The skin area is selected as the face in the test, and the tested object is required to be evenly smeared in the test process. The examinee needs to smear the pox-printed part under the regulation requirement, and the pox-printed part is smeared once every morning and evening, and the service cycle is 2 months. And in the testing stage, other cosmetics are stopped. The data were collected 4 times in total during the test, which were at initial use, first week, second week, and fourth week, respectively. The data acquisition and observation stage is the same time period every day. The test results are shown in Table 8, and as shown in Table 8, the acne marks on the skin of the test group become obviously lighter and gradually heal, the period of completely eliminating the acne marks is 25 days, while the acne marks of the skin of the test group are eliminated for a longer period than those of the skin of the test group without sea buckthorn water. 80.0% of the subjects had significantly reduced acne marks, and as shown in FIG. 5, the skin of one of the patients had significantly reduced acne marks after one month of administration of the sea buckthorn water of the present invention.
TABLE 8 Effect on healing of skin acne marks
Figure BDA0002428301750000131
The results of comparing the curative effects of the test group and the control group are shown in the following table 9, the number of people who have lightened skin acne marks is obviously more than that of the control group by applying seabuckthorn lotion, the cure rate of the acne marks reaches 62.85 percent and is obviously higher than that of the control group, the seabuckthorn lotion is further proved to have the function of dispelling and lightening the skin acne marks, and the skin becomes more delicate after the seabuckthorn lotion is used.
TABLE 9 comparison of the therapeutic effects of two groups of patients with acne marks on skin
Figure BDA0002428301750000141
(4) Against the influence of UVC radiation
HaCaT cells are cultured, the tests are divided into groups and subjected to UVC radiation, the cell survival rate is measured by a CCK-8 method, the result is shown in table 10, and the control group refers to the test group which is not subjected to ultraviolet radiation; UVC group is the test group subjected to ultraviolet radiation; the 2mL group of the sea buckthorn water is used for observing the survival state of cells by smearing 2mL of sea buckthorn water through ultraviolet radiation; the 20mL group of seabuckthorn water refers to observing the survival state of cells by smearing 20mL of seabuckthorn water through ultraviolet radiation.
TABLE 10 Effect of Hippophae rhamnoides Water on apoptosis Rate of UVC radiation HaCaT cells
Figure BDA0002428301750000142
Remarking: superoxide dismutase (SOD); glutathione peroxidase (GSH-PX); catalase (CAT).
The number of cells in each group upon UV irradiation varied as follows: the control group showed good cell growth, while the uv-irradiated model group showed a decreased cell viability relative to normal cells. As shown in FIG. 6, the test groups containing 2mL and 20mL of sea buckthorn water showed a decrease in cell death rate and an increase in survival rate, compared to the test group containing UV irradiation. The survival rate of the two sea buckthorn test groups is obviously higher than that of the two sea buckthorn test groups with large amount.
(5) Sea buckthorn water whitening effect
In the test, 30 subjects (without allergy history) with the age of 25-50 years are selected, wherein 15 men and 15 women are selected. The tested part is the part of the forearm of the human body close to the wrist, wherein the left arm is a test object coated with sea buckthorn water, and the right arm is a reference object. The duration of use is 3 months, which is 1 month, 2 months and 3 months respectively before the test. Testing each tested part of the tested subject by using a Lab colorimetric tester, observing the skin color change of the tested part, and averaging the measurement results
Figure BDA0002428301750000151
(Δ E represents color difference Δ Eab) determined as shown in Table 11.
TABLE 11 color difference comparison of test sites of subjects at different time periods
Figure BDA0002428301750000152
From the above table it can be seen that: after the use for 1 month, the left arm color difference is obvious from the right arm color difference, and the number of people accounts for 60 percent. After 2 months of use, the left arm color difference is obvious from the right arm color difference, and the number of people accounts for 67%. After 3 months of use, the left arm color difference is obvious more than the right arm color difference, and the percentage of people is 77%. Therefore, the sea buckthorn water prepared by the invention is beneficial to whitening skin and has whitening effect.
(6) Safety feature
The results of the detection of the microorganism content of the present invention are shown in Table 12.
TABLE 12 microorganisms and hygiene indicators for sea buckthorn water
Figure BDA0002428301750000161
Figure BDA0002428301750000171
From table 12, it can be seen that: the number of the sea-buckthorn water flora does not exceed the standard and heavy metal ions and other harmful ions are not detected, so that the sea-buckthorn aquatic product has high safety.
The application of the sea buckthorn water of the invention is as follows: the sea buckthorn water has the effects of maintaining beauty, keeping young, resisting inflammation and sterilizing, can be applied to the cosmetic fields of facial masks, facial cream, toning lotion, essence and the like, and the application of the sea buckthorn water in the cosmetic field is described by taking the facial masks, the toning lotion, the emulsion and the essence as examples.
The method for preparing the repairing and nourishing mask by utilizing the sea buckthorn water comprises the following steps:
step 1, mixing the sea buckthorn water: propylene glycol: sodium hyaluronate: glycerol: corn gluten amino acids: beta-glucan: oligopeptide-1: sodium acrylate/sodium acryloyldimethyl taurate copolymer: allantoin: carbomer: triethanolamine: the ratio of bis (hydroxymethyl) imidazolidinyl urea is: weighing the raw materials according to the weight ratio of 70.6:5.1:5.1:4.3:2.6:2.6:8.9:0.2:0.2:0.2:0.1:0.1, and putting the weighed raw materials into a sterilized and disinfected vessel.
And 2, adding the propylene glycol, the glycerol, the sodium hyaluronate, the carbomer, the corn gluten amino acids and the allantoin weighed in the step 1 into a sterilized emulsifying pot, stirring and heating to 80-85 ℃, and continuing stirring for 40-45 minutes after all solutes are dissolved.
And 3, uniformly mixing the sea buckthorn water obtained by the method with the sodium acrylate/sodium acryloyldimethyl taurate copolymer at normal temperature, then adding the mixture into the emulsifying pot obtained in the step 1, starting stirring for 3 minutes, cooling the emulsifying pot to 55 ℃, adding triethanolamine and stirring uniformly.
And 4, cooling the emulsifying pot in the step 3 to 45 ℃, adding the beta-glucan, the oligopeptide-1 and the bis (hydroxymethyl) imidazolidinyl urea, and uniformly stirring.
And 5, cooling the emulsifying pot to 38 ℃ in the step 4, taking a small sample for detection, preparing the sea-buckthorn water restoration and nourishing facial mask by using the human-skin-imitated facial mask after the detection is qualified, wherein the appearance detection of the facial mask is shown in a table 13.
TABLE 13 sensory indices of facial mask
Figure BDA0002428301750000181
The application method of the facial mask comprises the following steps: the face is cleaned, then the facial mask is applied to the face for 15-20 min, and then the face is cleaned with warm water. Selecting 30 test persons, continuously using the mask for 3 days, and testing the results as shown in a table 14.
TABLE 14 comparison of skin conditions of fructus Hippophae facial mask after application
Group of Change in skin condition
Common face pack No obvious change of skin state
Sea-buckthorn water mask The skin becomes tender and smooth, and the fine lines of the skin become obviously shallow
In the invention, the preparation flows of the common facial mask and the sea buckthorn water facial mask are the same, but the water in the preparation process of the common facial mask is distilled water, the sea buckthorn water facial mask replaces the distilled water with the sea buckthorn water, the test results are shown in table 15, and the test results are shown in table 15: the sea-buckthorn water mask prepared by the invention can obviously make the skin of a subject become tender and the fine wrinkles become shallow, and has a good water replenishing effect.
The skin moisture content of the test subjects in the control group and the test group was measured with a moisture meter every 5 minutes, and the measurement results are shown in fig. 7, from which it is clear that: the relative facial skin moisture values of the control group and the test group are the same when the mask is just applied, the relative facial skin moisture values of the control group and the test group tend to increase along with the increase of the mask application time, and the relative facial skin moisture values of the control group and the test group tend to be stable in 20 minutes, but obviously, the moisturizing effect of the seabuckthorn water mask prepared by the invention is superior to that of a common mask, so that the moisturizing, wrinkle removing and whitening effects of the seabuckthorn water mask prepared by the invention are obviously superior to that of the common mask.
(II) the method for preparing the sea-buckthorn toner by utilizing the sea-buckthorn water comprises the following steps:
step 1, mixing the sea buckthorn water: propylene glycol: sodium hyaluronate: glycerol: corn gluten amino acids: oligopeptide-1: allantoin: carbomer: the triethanolamine ratio was 82.2: 5.1:5.1:4.3:2.6: 0.2:0.2:0.2: the raw materials were weighed in an amount of 0.1, and the weighed raw materials were put in a sterilized and disinfected dish.
And 2, adding the propylene glycol, the glycerol, the sodium hyaluronate, the carbomer, the corn gluten amino acids and the allantoin weighed in the step 1 into a sterilized emulsifying pot, stirring and heating to 84 ℃, and continuing stirring for 40-45 minutes after all solutes are dissolved.
And 3, step 3: adding sea buckthorn water and the sodium acrylate/sodium acryloyldimethyl taurate copolymer under the premise of the step 2, homogenizing for 5 minutes, keeping for 30 minutes, cooling the emulsifying pot to 45 ℃, adding triethanolamine, and stirring uniformly. Standing, discharging, inspecting, and packaging after qualified inspection.
The use method of the sea buckthorn toner comprises the following steps: cleaning face, and then applying the sea buckthorn water toner on cheek. Selecting 30 test users, continuously using for 1 week, wherein the test results are shown in a table 14, and the following results can be obtained from the table 14: the appearance and the fragrance of the sea buckthorn water toner prepared by the invention both accord with the regulations of the national relevant standards.
TABLE 14 sensory indices of Hippophae rhamnoides toner
Figure BDA0002428301750000191
Figure BDA0002428301750000201
TABLE 15 comparison of skin condition of the applied sea buckthorn water toner
Group of Change in skin condition
Common toner No obvious change of skin state
Sea-buckthorn toner The skin becomes tender and the fine lines become shallow
In the invention, the preparation flows of the common toner and the sea buckthorn water toner are the same, the water in the preparation process of the common toner is distilled water, the sea buckthorn water toner is distilled water instead of distilled water, the test results are shown in table 15, and the test results are shown in table 15: the sea-buckthorn water toner prepared by the invention can obviously make the skin of a subject become tender and the fine lines become shallow, and has a good water replenishing effect.
(III) the preparation method of the sea buckthorn water moisturizing emulsion comprises the following steps:
step 1, mixing the sea buckthorn water: propylene glycol: sodium hyaluronate: glycerin: corn gluten amino acids: beta-glucan: oligopeptide-1: sodium acrylate/sodium acryloyldimethyl taurate copolymer: allantoin: carbomer: triethanolamine: the ratio of bis (hydroxymethyl) imidazolidinyl urea is: weighing the raw materials according to the weight ratio of 70.6:5.1:5.1:4.3:2.6:2.6:8.9:0.2:0.2:0.2:0.1:0.1, and putting the weighed raw materials into a sterilized and disinfected vessel.
And 2, adding the propylene glycol, the glycerol, the sodium hyaluronate, the carbomer, the corn gluten amino acids and the allantoin weighed in the step 1 into a sterilized emulsifying pot, stirring and heating to 84 ℃, and continuing stirring for 40-45 minutes after all solutes are dissolved.
And 3, adding sea buckthorn water and the sodium acrylate/sodium acryloyldimethyl taurate copolymer on the premise of the step 2, homogenizing for 5 minutes, keeping for 30 minutes, cooling the emulsifying pot to 45 ℃, adding triethanolamine, beta-glucan, oligopeptide-1 and bis (hydroxymethyl) imidazolidinyl urea, and uniformly stirring.
And 4, cooling the emulsifying pot to 35-40 ℃ in the step 3, taking a small sample for detection, and packaging after the detection is qualified.
The application method of the sea buckthorn aqueous emulsion comprises the following steps: the face is cleaned, and then the sea-buckthorn aqueous emulsion is smeared on the cheek. 30 subjects were selected and used continuously for 1 week, and sensory results of aqueous emulsion of Hippophae rhamnoides are shown in Table 16.
TABLE 16 sensory indices of aqueous emulsion of Hippophae rhamnoides
Serial number Inspection item Index (I) Test results
1 Appearance of the product Compliance with regulations Qualified
2 Fragrance Compliance with regulations Qualified
TABLE 17 comparison of skin conditions of the moisturizing lotion of Hippophae rhamnoides after application
Group of Changes in skin condition
Ordinary moisturizing emulsion No obvious change of skin state
Aqueous emulsion of seabuckthorn fruit The skin becomes tender and moist, and the fine wrinkles become shallow
In the invention, the preparation flows of the common moisturizing emulsion and the sea-buckthorn aqueous emulsion are the same, distilled water is adopted in the preparation process of the common moisturizing emulsion, and the sea-buckthorn aqueous emulsion replaces the distilled water with the sea-buckthorn water. The test results are shown in Table 17, and it can be seen from Table 17 that: the sea-buckthorn aqueous emulsion prepared by the invention can obviously make the skin of a subject become tender and moist and thin wrinkles become shallow.
(IV) the preparation method of the sea buckthorn water essence comprises the following steps:
step 1, mixing the sea buckthorn water: butanediol: sodium hyaluronate: glycerol: hydrolyzing yeast protein: oligopeptide-1: sodium acrylate/sodium acryloyldimethyl taurate copolymer: collagen: carbomer: triethanolamine: hydroxyphenyl esters are 69.2: weighing the raw materials according to the weight ratio of 5.4:5.4:4.6:2.8:9.4:0.2:2.5:0.2:0.1:0.1, and putting the weighed raw materials into a sterilized vessel.
And 2, adding the butanediol, the glycerol, the sodium hyaluronate, the carbomer and the hydrolyzed yeast protein weighed in the step 1 into a sterilized emulsifying pot, stirring and heating to 84 ℃, and continuing stirring for 30-45 minutes after all solutes are dissolved.
And 3, adding sea buckthorn water and the sodium acrylate/acryloyl dimethyl taurate copolymer under the premise of the step 2, homogenizing for 5 minutes, keeping for 25 minutes, cooling the emulsifying pot to 45 ℃, adding triethanolamine, collagen, oligopeptide-1 and propylparaben, and uniformly stirring.
The use method of the sea buckthorn water essence comprises the following steps: cleaning face, applying conventional cosmetic water and lotion on skin, and applying the essence solution on cheek. 30 test users are selected and used for 1 week continuously, the test results are shown in a table 18, and the following results can be obtained from the table 18: the appearance and the fragrance of the sea buckthorn water toner prepared by the invention both meet the regulations of the national relevant standards.
TABLE 18 sensory indices of aqueous extract and liquid of Hippophae rhamnoides
Serial number Inspection item Index (es) Test results
1 Appearance of the product Compliance with regulations Qualified
2 Fragrance Compliance with regulations Qualified
TABLE 19 comparison of skin conditions of fructus Hippophae water essence after application
Group of Changes in skin condition
Common essence No obvious change of skin state
Sea-buckthorn water essence The skin becomes more moist and tender
In the invention, the preparation flows of the common essence and the sea buckthorn water essence are the same, distilled water is adopted in the preparation process of the common essence, the sea buckthorn water essence replaces the distilled water with the sea buckthorn water, the test results are shown in table 19, and the table 19 shows that: the skin of a subject can be obviously moisturized and tender by the sea-buckthorn water toner prepared by the invention, which shows that the sea-buckthorn water essence prepared by the invention has better effects of moisturizing, brightening and moistening the skin.

Claims (1)

1. A sea buckthorn water is characterized in that the sea buckthorn water is extracted from sea buckthorn plants; the preparation method of the sea buckthorn water is specifically implemented according to the following steps:
step 1, cleaning and pretreating sea buckthorn plants;
the seabuckthorn plants in the step 1 comprise seabuckthorn seeds and seabuckthorn dry fruits;
the pretreatment process of the seabuckthorn seeds or the dried seabuckthorn fruits in the step 1 is crushing and tabletting, the thickness of the tablets is 0.3-0.5 mm, and the crushed tablets are smaller than 18 meshes;
step 2, extracting the raw materials obtained in the step 1 to obtain the sea buckthorn water;
the seabuckthorn seeds/dried seabuckthorn fruits in the step 2 are extracted by supercritical carbon dioxide,
the specific process of extracting the sea buckthorn seeds/dried sea buckthorn fruits by adopting supercritical carbon dioxide comprises the following steps: the raw material after tabletting is carried out at the pressure of 270-290 bar, the temperature of 52-55 ℃ and CO 2 Extracting for 4.5-5.5 h under the condition that the flow is 2750-2850kg/h, performing primary separation under the conditions that the pressure is 100-110 bar and the temperature is 45-50 ℃ after extraction, performing secondary separation under the conditions that the pressure is 50-55 bar and the temperature is 30-35 ℃, and standing and separating oil-water substances obtained through separation under the conditions that the temperature is 40-45 ℃ and the time is 5h to obtain the sea buckthorn water.
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