CN111323573A - Sample adding indicating strip and incubation disc with same - Google Patents

Abstract

The invention relates to a sample adding indicator strip, which comprises: a bottom strip configured to be adapted to be disposed on a carrier having a plurality of dosing slots; the indicating holes are arranged on the bottom strip, and the positions of the indicating holes correspond to the liquid adding grooves of the bearing device; wherein the plurality of indicating holes comprise indicating materials which are suitable for indicating whether the indicating materials are contacted with the liquid added into the liquid adding groove. The application greatly reduces the phenomena of missing addition and wrong addition in the addition process, reduces the error probability in the addition process, and ensures the accuracy of the experimental result. Meanwhile, the method has high operability, can improve the experiment efficiency, saves time and reduces the experiment loss.

Description

A sample loading indicator strip and an incubation plate with the sample loading indicator strip

technical field

The invention relates to an experimental consumable, in particular to a sample loading indicator strip and an incubation plate with the sample loading indicator strip.

Background technique

Blotting experiments are usually carried out in incubation trays, which are divided into 30 wells/incubation trays, 44 wells/incubation trays, 48 wells/incubation trays and 50 wells/incubation trays according to different specifications. Wrong addition and so on.

Fully ensuring that the sample addition process is correct is the most effective way to ensure accurate test results and reduce experimental losses, and it is also the most direct manifestation of being responsible to patients. At present, laboratories often use a marker pen to mark the sample number or component code on the incubation plate to avoid mistakes. Although this method can achieve a certain effect of avoiding errors, in the case of a huge sample size, this method is not very feasible and inefficient, and at the same time, it is inevitable to add wrong or missing samples or components. .

SUMMARY OF THE INVENTION

In view of the technical problems existing in the prior art, the present invention proposes a sample adding indicator strip, comprising: a bottom strip, which is configured to be suitable for being placed on a carrying device having a plurality of liquid adding grooves; and a plurality of indicator holes , the plurality of indicator holes are arranged on the bottom strip, wherein the positions of the plurality of indicator holes correspond to the plurality of liquid filling grooves of the carrying device; wherein, the plurality of indicator holes include indicator materials, which are suitable for to indicate whether it is in contact with the liquid added to the dosing tank.

The sample loading indicator strip as described above further comprises a waterproof layer covering the bottom strip.

The sample adding indicator strip as described above, wherein the indicator material is anhydrous cobalt chloride.

The sample loading indicator strip as described above, wherein above the plurality of indicator holes includes an anti-contamination material.

The sample loading indicator strip as described above, wherein the anti-pollution material is cotton fiber or filter paper fiber.

The sample loading indicator strip as described above, wherein the side of the bottom strip without the indicator hole comprises a back glue.

According to another aspect of the present invention, a sample application indicator strip roll is provided, comprising the sample application indicator strip as described above.

According to another aspect of the present invention, an incubation tray is provided, comprising: a tray body; a plurality of liquid addition grooves, which are arranged on the tray body; The positions of the plurality of indication holes of the sample addition indicator strip correspond to the plurality of liquid addition grooves.

According to another aspect of the present invention, a method for using a sample adding indicator strip is provided, comprising: cutting out a part of the sample adding indicator strip described above; and cutting out a part of the sample adding indicator strip described above; The strip is affixed to the incubation plate so that the positions of the plurality of indicator holes of the sample addition indicator strip as described in any of the above correspond to the plurality of addition slots of the incubation plate.

According to another aspect of the present invention, there is provided a method for adding liquid by using the sample adding indicator strip as described above, comprising: before or after adding liquid to the liquid addition tank, adding liquid for adding liquid The head contacts the indicator hole of any of the above-mentioned sample addition indicator strips corresponding to the liquid addition tank.

According to another aspect of the present invention, there is provided a method for adding liquid using the above-mentioned incubation tray, comprising: before or after adding liquid to the liquid addition tank, contacting a liquid addition head for liquid addition with the liquid addition tank. The indicator hole of any one of the above-mentioned sample addition indicator strips corresponding to the liquid addition tank.

The present application greatly reduces the phenomenon of missed addition and wrong addition in the sample addition process, reduces the error probability in the sample addition process, and ensures accurate experimental results. At the same time, it has high operability, which can improve experimental efficiency, save time and reduce experimental loss.

Description of drawings

Below, the preferred embodiments of the present invention will be described in further detail in conjunction with the accompanying drawings, wherein:

FIG. 1A is a front view of a sample addition indicator strip according to an embodiment of the present invention;

FIG. 1B is a top view of a sample addition indicator strip according to an embodiment of the present invention;

1C is a partial enlarged view of a sample adding indicator bar according to an embodiment of the present invention;

FIG. 2 is a roll of a splice indicator strip according to one embodiment of the present invention; and

Fig. 3 is a schematic diagram of the use of a sample loading indicator strip on an incubation plate according to an embodiment of the present invention.

Detailed ways

In order to make the purposes, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments These are some embodiments of the present invention, but not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

In the following detailed description, reference may be made to the accompanying drawings, which are considered a part of this application to illustrate specific embodiments of the application. In the figures, like reference numerals describe substantially similar components in the different figures. The specific embodiments of the present application are described in sufficient detail below to enable those of ordinary skill with relevant knowledge and technology in the art to implement the technical solutions of the present application. It should be understood that other embodiments may also be utilized or structural, logical or electrical changes may be made to the embodiments of the present application. In this application, the same reference numerals denote the same structures.

The application provides a sample loading indicator strip, which can be used to incubate a plate and perform experiments such as immunodetection. The structure is simple and the operability is strong, which can effectively improve the experimental efficiency and save experimental consumables.

1A-1C show a sample adding indicator bar according to an embodiment of the present application; wherein, FIG. 1A is a schematic structural diagram of the sample adding indicator bar, and FIG. 1B is a structural schematic diagram of another angle of the sample adding indicator bar. 1C is a partial enlarged view of the sample loading indicator strip. As shown in FIGS. 1A-1C , the sample adding indicator strip 100 (sometimes referred to herein as indicator strips, both of which have the same meaning) includes: a bottom strip 110 , a plurality of indicator holes 121 - 128 and an indicator material 130 . The bottom strip 110 is adapted to be placed on a carrier having a plurality of liquid addition tanks, such as an incubation tray. A plurality of indicator holes 121-128 are arranged on the bottom strip 110, and their positions correspond to the liquid filling grooves on the carrying device one by one. The plurality of indicator holes 121-128 include an indicator material 130, which is adapted to indicate whether it is in contact with the liquid added to the liquid addition tank, thereby indicating whether reagent addition has been completed in a liquid addition tank.

As shown in FIGS. 1A and 1B , the bottom strip 110 is in the shape of a strip. The surface of the bottom strip 110 can withstand a certain degree of attack by water molecules, so that it has a stable shape, and is not deformed due to contamination by liquid reagents, and has good durability, so as to extend its shelf life and service life to the greatest extent. In some embodiments, the bottom strip 110 has good flexibility and can be placed in a roll (refer to FIG. 2 ) for convenient storage. In some embodiments, the bottom strip 110 is made of a waterproof material, such as waterproof paper. In some embodiments, a waterproof layer 160 is attached to the bottom strip 110 . In some embodiments, the waterproof layer 160 is a film, and the film has good flexibility and water resistance to prevent the bottom strip 110 and the indicator material 130 from getting wet.

In some embodiments, the width H of the bottom strip 110 is 3-7 mm. For example, the width H of the bottom strip 110 may be about 5 mm to facilitate its use with the carrying device.

As shown in FIG. 1A and FIG. 1B , the indicator holes 121 - 128 are provided on the bottom strip 110 and have a concave structure. In some embodiments, the indicator holes 121 - 128 are recessed downward by the bottom strip 110 . In some embodiments, the indicator holes 121 - 128 are formed by pressing the bottom strip 110 with a hard object. In some embodiments, the indicator holes 121 - 128 are formed by etching on the bottom strip 110 . In other embodiments, the indicator holes 121-128 may also be horizontal or convex. Without limitation, the concave structure is more conducive to storing the indicator material, and is a more preferred solution.

The indicator holes 121-128 are arranged along the long side of the bottom bar 110, the centers of the plurality of indicator holes 121-128 are arranged on a straight line, and the center line is parallel to the long side of the bottom bar 110, so that the indicator bar 100 is beautiful, The indicator bar 100 can also be tailored according to the size of the equipment used with it. In some embodiments, the indicator holes 121 - 128 are equidistant from both sides of the broad side of the bottom strip 110 .

In some embodiments, the indicator holes 121-128 are dotted slots. The distance L between adjacent indicator holes is equal, about 7-12mm, and the distance is the same as the distance between adjacent liquid filling tanks on the carrying device. In some embodiments, the distance L between adjacent indicator holes is about 9 mm.

In some embodiments, the indicator holes 121-128 are approximately 1.0-3.0 mm in diameter. For example, the indicator holes 121-128 may be approximately 1.5 mm in diameter. The size of the indicator holes 121-128 is convenient for the experimenter to touch with the pipette tip of the pipette gun 300 and can accommodate a small amount of liquid reagents. The diameter and depth of the indicator holes 121-128 are not limited in this experiment.

In some embodiments, indicator material 130 is included within the grooves of indicator holes 121-128. By indicating the change of material properties, the corresponding reagent addition in the liquid addition tank is characterized. In some embodiments, the indicator material 130 is anhydrous cobalt chloride. Anhydrous cobalt chloride is blue, hygroscopic, and turns pink or red after absorbing water. Its color changes quickly, and it can timely indicate whether the reagent is added in the corresponding liquid addition tank on the carrier device. In some embodiments, the indicator material 130 is added on the surface of the waterproof layer 160 inside the indicator hole to prevent the indicator material 130 from being denatured by moisture.

According to an embodiment of the present application, the indicator holes 121-128 may be covered with an anti-pollution material 170. In some embodiments, the antifouling material is cotton fibers; in other embodiments, the antifouling material is filter paper fibers. The anti-pollution material can not only isolate the indicator material 130 from contact with water molecules in the air, prevent the indicator material from absorbing moisture in the air and denature during storage, but also ensure that water molecules can quickly penetrate into the indicator holes 121-128 during the experiment. In contact with the indicator material 130 .

According to an embodiment of the present application, the indicator strip 100 further includes a back glue 140 for adhering the indicator strip to the carrying device. The adhesive 140 is attached to the side of the bottom strip 110 without the indicator holes 121-128. In some embodiments, the adhesive backing 140 is a viscous non-marking adhesive material, so that the indicator strip 100 can be firmly adhered to the surface of the carrier device, and the indicator bar 100 can be torn off from the carrier device at any time without leaving traces. In some embodiments, the surface of the adhesive backing 12 may be covered with a material such as smooth plastic paper, which can be completely separated from the adhesive backing and has good flexibility, so as to facilitate rolling up and storage of the indicator strip containing the adhesive backing.

Figure 2 is a roll of a splice indicator strip according to one embodiment of the present invention. As shown in FIG. 2 , in some embodiments, the indicator strip 100 can be rolled into an indicator strip roll, which is convenient for storage. When using, it is only necessary to cut off the required length of the indicator bar on the indicator bar roll.

According to an embodiment of the present application, an indicator strip can be attached to an incubation plate, wherein the incubation plate can be a disposable experimental consumable, or an experimental consumable that can be reused many times. The incubation plate includes a plate body 120 and a plurality of liquid addition grooves 221-2215, the plurality of indicator holes 121-1215 on the indicator bar correspond to the plurality of liquid addition grooves 221-2215 on the incubation plate respectively, and each indicator hole indicates its corresponding Whether liquid is added to the dosing tank.

In some embodiments, the indicator strip is integrated with the incubation plate, and the incubation plate with the indicator strip is a disposable experimental consumable. As shown in FIG. 3 , the incubation plate with indicator strips includes a plurality of indicator holes 121-1215, a plate body 210 and a plurality of sample addition slots 221-2215. The plurality of indication holes 121-1215 are in one-to-one correspondence with the plurality of liquid addition tanks 221-2215, and each indication hole indicates whether liquid is added to the corresponding liquid addition tank.

According to an embodiment of the present application, a method for using a sample adding indicator strip includes:

1. Intercept a part of the sample adding indicator strip as mentioned above;

2. Paste the cut sample loading indicator strip on the incubation plate, so that the positions of the multiple indicator holes of the sample loading indicator strip correspond to the multiple liquid adding grooves of the incubation plate (as shown in Figure 3).

According to an embodiment of the present application, the method for adding liquid using the above-mentioned sample adding indicator strip includes: before or after adding liquid to the liquid addition tank, contacting a liquid addition head for liquid addition with liquid addition The indicator hole of the sample adding indicator bar corresponding to the slot.

According to an embodiment of the present application, the method for adding liquid by using the above-mentioned incubation plate includes: before or after adding liquid into the liquid adding tank, contacting the liquid adding head for adding liquid with a liquid adding tank corresponding to the liquid adding tank. The indicator hole of the sample loading indicator strip.

As shown in Figure 3, in some embodiments, when using the indicator hole to indicate the addition of the liquid reagent corresponding to the liquid addition tank, the following steps may be referred to:

1. Truncate an indicator strip of moderate length. Wherein, the number of indication holes in the intercepted indication bar should be the same as the number of liquid addition tanks on the device to be indicated.

2. Paste the indicator strip on the incubation plate. Before sticking firmly, adjust the position of the indicator strip so that each indicator hole corresponds to each sample filling slot.

3. Add the reagents to the liquid addition tank, and mix the reagents by blowing and sucking according to the experimental requirements.

4. Without replacing the liquid filling head of the pipette, directly use the top of the liquid filling head of the pipette for adding reagents to contact the corresponding indicator hole.

5. The indication hole changes in character, for example, the color of the indication hole changes from blue to red, indicating that the addition of the sample to the liquid addition tank has been completed.

Wherein, in some embodiments, step 3 and step 4 can be interchanged, first touch the indicator hole with the liquid filling tip of the pipette that absorbs the reagent, and then add the reagent into the corresponding liquid addition tank.

According to an embodiment of the present application, as shown in FIG. 2 , each time a liquid reagent is added to a liquid addition tank, immediately use the liquid addition tip of the sample addition pipette to touch the corresponding indicator hole. For example, the liquid addition tanks 221-224 and the indicator holes 121-124 have respectively added reagents correspondingly, and the indicator holes 121-124 have changed. After adding the reagent into the liquid addition tank 225, immediately use the pipette tip of the pipette gun 300 for adding the reagent to touch the corresponding indicator hole 125. When the indicator hole 125 changes from blue to pink, it indicates that the liquid addition tank 225 The liquid addition has been completed, and the liquid addition tanks 226-2215 are to be added.

This method can ensure that sample reagents are added to each sample addition tank without repeated additions. Even if there is a short pause, the continuous sample addition position can be easily identified, and no additional consumables are required, saving costs.

The above-mentioned embodiments are only for the purpose of illustrating the present invention, rather than limiting the present invention. Those of ordinary skill in the relevant technical field can also make various changes and modifications without departing from the scope of the present invention. Therefore, all Equivalent technical solutions should also belong to the scope of the disclosure of the present invention.

Claims (11)

1. A sample adding indicator strip, comprising: a bottom strip configured to be suitable for placement on a carrier having a plurality of refill tanks; and a plurality of indication holes, the plurality of indication holes are arranged on the bottom strip, wherein the positions of the plurality of indication holes correspond to the plurality of liquid filling grooves of the carrying device; wherein, the plurality of indication holes include indications material, which is suitable to indicate whether it is in contact with the liquid added to the dosing tank. 2. The sample loading indicator strip of claim 1, further comprising a waterproof layer covering the bottom strip. 3. The sample loading indicator strip according to claim 1, wherein the indicator material is anhydrous cobalt chloride. 4. The sample loading indicator strip of claim 1, wherein an anti-contamination material is included above the plurality of indicator holes. 5. The sample loading indicator strip according to claim 4, wherein the anti-contamination material is cotton fiber or filter paper fiber. 6. The sample loading indicator strip according to claim 1, wherein the side of the bottom strip without the indicator hole comprises a back glue. 7. A sample application indicator strip volume, comprising the sample application indicator strip according to any one of claims 1-6. 8. An incubation plate comprising: body; a plurality of liquid adding tanks, which are arranged on the disc body; and The sample adding indicator strip according to any one of claims 1-6; Wherein, the positions of the plurality of indication holes of the sample addition indicator strip according to any one of claims 1-6 correspond to the plurality of liquid addition grooves. 9. A method of using a sample adding indicator strip, comprising: A truncated part of the sample addition indicator strip according to any one of claims 1-6; and A part of the sample loading indicator strip according to any one of claims 1-6 is cut out and pasted on the incubation plate, so that the positions of the plurality of indicator holes of the sample loading indicator strip according to any one of claims 1-6 are the same as those described in the above. The multiple addition tanks of the incubation plate correspond to each other. 10. A method for adding liquid using the sample adding indicator strip as described in any one of claims 1-6, comprising: before or after adding liquid in the liquid adding tank, contacting a liquid addition head for liquid addition The indicator hole of the sample addition indicator strip according to any one of claims 1-6 corresponding to the liquid addition tank. 11. A method for adding liquid using the incubation plate according to claim 8, comprising: before or after adding liquid to the liquid adding tank, contacting a liquid adding head for adding liquid with a liquid adding head corresponding to the liquid adding tank. The indicator hole of the sprinkling indicator strip according to any one of claims 1-6.

Images