JPH0320761Y2 - - Google Patents

Description

[Detailed Description of the Invention] [Industrial Field of Application] This invention relates to an instrument for staining optical microscope specimens, and in particular, to an instrument for staining optical microscope specimens that utilizes capillarity.

[Problems to be solved by conventional techniques and ideas]

In conventional immunostaining, in one staining process, the moisture around each specimen is wiped off several times, and the staining solution is applied onto each glass slide. I have been working on adding it dropwise and stirring it. As a result, a huge amount of time was consumed, and accidents involving damage to specimens often occurred.

However, the staining reaction time was not constant, and furthermore, since the reaction was stopped and the degree of reaction promotion was observed under a microscope, it was almost impossible to maintain a constant staining result.

[Means for solving problems]

This invention was proposed in order to solve the problem described above, and is equipped with a cross-sectional guide 1, and a sliding piece 2 is placed inside the guide 1 so as to be slidable in the longitudinal direction. , this sliding piece 2
Further, on the surface of the upright piece on both sides of the guide 1, recesses 1c, 1c, etc. are provided facing each other at regular intervals in the width direction, and the facing recesses 1c, The slide glasses 4, 4... can be inserted into the slide glasses 1c, respectively, and a small gap is formed between the lower surface of the slide glasses 4, 4... and the upper surface of the sliding piece 2. It is an object of the present invention to provide an apparatus for staining optical microscope specimens that utilizes capillary phenomenon and is characterized by the following characteristics.

[Effect]

A sliding piece is inserted into the guide, and the staining liquid droplet formed on the sliding piece is positioned between the recesses provided in the width direction of the guide. After that, staining is applied to the staining liquid droplet. Add liquid (antibody, etc.) dropwise. Then, the slide glass with the staining object pasted thereon is inserted into the recesses provided on the surfaces of the upright pieces on both sides of the guide, with the pasting surface facing downward. Furthermore, after that, while sliding the sliding piece on the guide, the staining solution dripping portion is slid onto the staining target surface of the slide glass. When this happens, a liquid layer is formed in between. At this time, a liquid layer is easily formed due to capillarity and does not flow out. Furthermore, the reciprocating movement of the sliding piece is repeated to agitate the staining solution (antibody, etc.), and the staining solution (antibody, etc.) is allowed to stand still to promote the reaction.

〔Example〕

An embodiment of this invention will be described below in detail with reference to the accompanying drawings. Reference numeral 1 denotes a guide, which is formed in a cross-sectional shape, and both end surfaces thereof are also shaped and open. The material may be glass, stainless steel, various types, resin, etc., but should not be limited to these. Further, a large number of grooves 1a, 1a, . . . are formed on the upper bottom surface to reduce friction.
Further, recesses 1c, 1c, . . . are provided at regular intervals R in the upright pieces 1b, 1b on both sides, facing each other. The depth of the grooves 1c, 1c... is the same as that of the slide glasses 4, 4...
It is sufficient that they can be inserted into each other. Also, this guide 1
Support portions 1d, 1d, 1d, and 1d are protruded from both ends in the longitudinal direction when stacked on each other. Reference numeral 2 denotes a sliding piece, which may be made of the same material as the guide 1 described above. The sliding piece 2 is placed within the guide 1 and is formed to be able to slide within the guide 1. Therefore, the width of the sliding piece 2 is generally formed to be approximately equal to the width of the inner bottom surface of the guide 1, and although it is flat, the upper surface thereof has small grooves 3 in the width direction at regular intervals. It is equipped with a dyeing liquid droplet portion shaped like a number of 3.... Furthermore, ground glass surfaces 2a, 2a, etc. for writing memos are formed within the space between the staining liquid droplets, and concave notches 2b, 2b,... are provided in the width direction adjacent thereto to avoid unnecessary waste. Grooves are formed to prevent staining by staining solution. Although the thickness of this sliding piece 2 may be arbitrary, there is a small gap between its upper surface and the lower surface of the slide glass placed in the recesses 1c, 1c, . constructed and configured to be slidable. 4 is a slide glass. The width of this slide glass 4 is the recess 1c, 1 of the guide 1.
Any material that can be inserted into the hole c.c. Further, its length may be such that it protrudes outward from both the recesses 1c, 1c.

One embodiment of this invention has the configuration described above, so first, the sliding piece 2 is placed on the upper surface of the guide 1, and the dyeing liquid droplet portion formed on the upper surface of this sliding piece 2 is placed on the upper surface of the guide 1. They are positioned between the recesses 1c, 1c, . . . of the guide 1, respectively. Then, a staining solution (antibody, etc.) is dropped into the area where the staining solution is dropped. The dropping amount can be approximately 0.12 to 0.3 ml. Next, a tissue section to be stained is pasted on the lower surface of each of the slide glasses 4, 4.... Then, they are inserted into the recesses 1c, 1c, . . . of the guide 1, respectively, with the pasted portion β facing downward. In this case, the staining liquid drop portion α and the tissue section pasting portion β are not yet in contact with each other. Therefore, the sliding piece 2 is slid along the surface of the guide 1 until the dripping portions α and the sticking portions β come into contact with each other. In this case, a liquid layer is formed between the dripping portion α and the sticking portion β. At this time, a capillary phenomenon occurs between the dripping portion α and the lower surface of the slide glass, and the liquid layer can be easily formed without leaking to the outside. Furthermore, the staining solution (antibody, etc.) is stirred by repeating the reciprocating motion of the sliding piece 2 in small increments.
The small grooves 3, 3 are installed so that stirring can be carried out effectively. The reaction can then be accelerated by allowing it to stand still.

[Effect of idea]

Since this invention will be described in detail in one embodiment, complicated staining operations (mainly immunohistochemical staining, immunostaining and fluorescent antibody staining) can be performed easily and accurately.

(b) Edge work can be omitted.

(b) Staining solution stirring work can be simplified.

(c) It is possible to prevent specimen damage accidents caused by edging work and stirring work.

The evaporation of the staining liquid is prevented by significantly reducing the contact surface with the outside air.

Since the liquid layer is stable, it is possible to prevent the staining liquid from flowing out.

Staining time can be significantly shortened.

By using a transparent or translucent material for the guide, the state of staining can be observed under a microscope without stopping the reaction.

Since several specimens can be stained at the same time, substantially uniform staining results can be obtained.

[Brief explanation of the drawing]

The figures show one embodiment of this invention, in which Fig. 1 is an overall oblique view, Fig. 2 is a plan view of the guide, Fig. 3 is a front view of the same, Fig. 4 is a side view of the same, and Fig. 5 is a slider. FIG. 6 is a front view of the moving piece, FIGS. 7 and 8 are partially cutaway plan views showing how to use it, and FIG. 9 is a side view of the moving piece. Explanation of symbols, 1...Guide, 1a...Recess, 2...
...Sliding piece, 3...Small groove, 4...Slide glass.

Claims (1)

[Scope of utility model registration request] A cross-sectional guide 1 is provided, a sliding piece 2 is placed on the guide 1 so as to be slidable in the longitudinal direction, and a staining liquid dripping portion is provided on the surface of the sliding piece 2,
Further, on the surfaces of the upright pieces on both sides of the guide 1, there are recesses 1c, 1c facing each other at regular intervals in the width direction.
... is provided, and in these opposing recesses 1c, 1c...,
The slide glasses 4, 4... can be inserted into each other, and a small gap is formed between the lower surface of the slide glasses 4, 4... and the upper surface of the sliding piece 2. An instrument for staining specimens under an optical microscope that utilizes capillary action.