CN111263587B - Use of isotianil against Panama disease - Google Patents

Abstract

The present invention relates to the use of isotianil (formula (I)) (I) for controlling panama disease in plantago plants. Furthermore, the invention relates to a method for controlling panama disease by treating plants of the family musaceae with isotianil (I).

Description

Use of isotianil against Panama disease

The present invention relates to the use of isotianil (IST) (formula (I)) for controlling Panama disease in Musaceae plants.

Furthermore, the present invention also relates to a method for controlling Panama disease by treating Musaceae plants with isotianil or a formulation comprising isotianil (formula (I)).

Furthermore, the present invention also relates to mixtures of isotianil with at least one other active ingredient selected from fungicides, bactericides, for example to broaden the spectrum of action or prevent the development of resistance, for the treatment of Panama disease pesticides, acaricides, nematicides, herbicides, insecticides, safeners, host defense inducers, soil improvement products or products that reduce plant stress (eg Myconate).

In a preferred embodiment, the present invention relates to a mixture of isotianil and at least one other active ingredient selected from the group consisting of aluminum triethylphosphonate (Fosetyl -Al), as well as mono- and disodium phosphites, mono- and di-potassium phosphites and mono- and di-ammonium phosphites (eg Phostrol).

Compounds of formula (I) are known inter alia from WO 99/024413, WO 2006/098128, JP 2007-84566 and WO 96/29871.

invention

Panama disease is a fungal pathogen Fusarium oxysporum (Fusarium oxysporum), especially Fusarium oxysporum Cuba specialization (Fusarium oxysporum f.sp. Cuban (F0c)) race 1 and 4 caused by Invasive plant disease on the roots of banana plants. It is the most destructive disease of bananas. For example, in the 1950s, Panama disease decimated the production of most commercial Gros Michel (the dominant banana variety at the time) banana production. Today, a new strain of Panama disease is once again threatening yields of today's most popular cultivar, the Cavendish variety.

Fusarium fungi enter the roots of plants and spread through the plants' xylem vessels. The fungus damages the plant's vascular system, causing the leaves to yellow and wilt, eventually causing the plant to die.

To date, four races of the pathogen have affected banana crops worldwide. Its "race 1" has spread to the Philippines and Indonesia, where the more aggressive "tropical race 4" is now spreading. Race 1 has also spread in Africa and Australia. It has yet to reach Latin America, but it is only a matter of time when Panama disease will also destroy banana plantations in the region. This will cause huge economic losses and endanger the survival of the relevant banana farmers.

To date, Fusarium pathogens are resistant to fungicides and no chemical solutions are available.

In WO 2010/037482, isotianil derivatives are described for controlling microbial and animal pathogens in Musaceae plants, the only example being Mycosphaerella fijiensis.

It has now been found that isotianil and mixtures of isotianil and at least one other active ingredient are particularly suitable for controlling Panama disease on Musaceae plants.

Therefore, the first subject of the present invention is the use of isotianil for controlling Panama disease in Musaceae plants.

Therefore, another subject of the present invention is the use of isotianil for controlling Panama disease in plants of the genus Musa.

Another subject of the present invention is a method for controlling Panama disease in Musaceae plants, characterized in that the Musaceae plants are treated with isotianil.

Another subject of the invention is the above-mentioned use and method, wherein isotianil is used in combination with at least one other active ingredient selected from the group consisting of fungicides, Bactericides, acaricides, nematicides, herbicides, insecticides, safeners, host defense inducers, soil improvement products or products that reduce plant stress (eg Myconate).

A preferred embodiment of the present invention is the above-described use and method, wherein the F. oxysporum specialization type race 1 or race 4 causes Panama disease.

Another preferred embodiment of the present invention is the above-described use and method, wherein isotianil or a combination of isotianil and at least one other active ingredient is applied, wherein the other active ingredient is preferably selected from triethylphosphonic acid Aluminum, monosodium and disodium salts of phosphorous acid, monopotassium and dipotassium salts of phosphorous acid, and mono- and diammonium salts of phosphorous acid, more preferably aluminum triethylphosphonate.

Another preferred embodiment of the present invention is the above-mentioned use and method, wherein the method used is drip irrigation, preferably every 30 days, more preferably every 14 days using 2.5 to 0.5 g aluminium triethylphosphonate per plant and 0.035 to 0.015 IST /plant, more preferably 2.0 to 1.0 g aluminium triethylphosphonate/plant and 0.03 to 0.02 g IST/plant, even more preferably 2.0 to 1.0 g aluminium triethylphosphonate/plant and 0.03 to 0.02 g IST/plant and most preferred 1.6g aluminium triethylphosphonate/plant and 0.024g IST/plant.

Another preferred embodiment of the present invention is the above-described use and method, wherein isotianil is used in combination with aluminium triethylphosphonate in a ratio of 1 to 60 to 1 to 70, in % by weight.

Another preferred embodiment of the present invention is the above use and method, wherein the method used is drip irrigation, preferably every 30 days, more preferably every 14 days using 0.45 to 0.1 g aluminium triethylphosphonate per plant and 0.04 to 0.015 g IST/plant, more preferably 0.4 to 0.15 g aluminium triethylphosphonate/plant and 0.04 to 0.02 g IST/plant, even more preferably 0.35 to 0.25 g aluminium triethylphosphonate/plant and 0.035 to 0.025 g IST/plant and most 0.28 g aluminium triethylphosphonate/plant and 0.028 g IST/plant are preferred.

Another preferred embodiment of the present invention is the above-described use and method, wherein isotianil is used in combination with aluminium triethylphosphonate in a ratio of 1 to 5 to 1 to 15, in % by weight.

Another preferred embodiment of the present invention is the above use and method, wherein the method used is drip irrigation, preferably every 30 days, more preferably every 14 days using 0.035 to 0.015 g IST/plant, more preferably 0.03 to 0.02 g IST/ Plant, even more preferably 0.03 to 0.02 g IST/plant and most preferably 0.024 g IST/plant.

Another preferred embodiment of the present invention is the above use and method, wherein the method used is drip irrigation, preferably every 30 days, more preferably every 14 days using 0.04 to 0.015 g IST/plant, more preferably 0.04 to 0.02 g IST/ Plant, even more preferably 0.035 to 0.025 g IST/plant and most preferably 0.028 g IST/plant.

definition

Musabukensis、Musa campestris、红蕉(Musa coccinea Andrews)、红花蕉(Musa uranoscoposLour)、Musa exotica Valmayor、Musa fitzalanii、Musa flavida、Musa gracilis、Musahirta Becc.、岛山芭蕉(Musa insularimontana Hayata)、Musa jackeyi、Musa johnsii、手指蕉(Musa lawitiensis)、Musa lolodensis、Musa maclayi、Musa monticola、Musamuluensis、河口指天蕉(Musa paracoccinea)、Musa peekelii、Musa pigmaea Hotta、阿希蕉(Musa rubra)、Musa salaccensis、Musa splendida A.Chev.、Musa suratii、蕉麻(Musatextilis)：马尼拉麻蕉(Abacá)、Japanese hardy或fibre banana、香蕉(Musatroglodytarum)、Musa tuberculata、Musa violascens、巨芭蕉(Musa ingens)、Musaparadisiaca sapientm、Musa paradisiaca normali，和这些物种的杂交品种。The Musaceae family consists in particular of the following species: Musa acuminata, Musabalbisiana, Musa acuminata Colla (with varieties "Dwarf Cavendish", "Large Cavendish""GiantCavendish" and "Gros Michel), Dwarf Banana (Musa cavendishii Lamb.ex Paxt.), Musa malaccensis Ridl., Musa angcorensis Gagnep., Musa aurantiaca, Musa balbisiana, Musa seminifera Lour., Musabanksii F.Muell., Musa basjoo, Musa cheesmanii, Musa flaviflora Simmonds, Musa griersonii, Musa itinerans, Musa laterita, Musa mannii, Musa nagensium), Musa ochracea, Musa ornata Roxb., Musa siamea, Musa sikkimensis, Musa thomsonii Noltie, Musa velutina Wendl.&Drude, Musa alinsanaya, Musa beccarii, Musa boman , Musa

Musabukensis, Musa campestris, Musa coccinea Andrews, Musa uranoscopos Lour, Musa exotica Valmayor, Musa fitzalanii, Musa favida, Musa gracilis, Musahirta Becc., Musa insularimontana Hayata, Musa jackeyi, Musa johnsii, Musa lawitiensis, Musa lolodensis, Musa maclayi, Musa monticola, Musamuluensis, Musa paracoccinea, Musa peekelii, Musa pigmaea Hotta, Musa rubra, Musa salaccensis, Musa splendida A.Chev., Musa suratii, Musatextilis: Manila banana (Abacá), Japanese hardy or fiber banana, banana (Musatroglodytarum), Musa tuberculata, Musa violascens, giant plantain (Musa ingens), Musaparadisiaca sapientm, Musa paradisiaca normali, and hybrids of these species.

Examples of fungi of the genus Fusarium that cause Panama disease in Musaceae plants are Fusarium spp, such as Fusarium pallidoroseum, Fusarium solani anamorph, dinoflagellate C. Nectria haematococca), Fusarium oxysporum, Fusarium moniliforme genotypes: Gibberella fujikuroi, Fusarium oxysporum f.sp.cubense ( Foc)), in particular races 1 and 4 of Foc, more particularly race 4 of Foc.

According to the present invention, the abovementioned isotianil or isotianil mixtures are used in particular against fungi of the genus Fusarium spp, such as Fusarium pallidoroseum, which cause Panama disease in Musaceae plants ), Fusarium solani anamorph, Nectriahaematococca, Fusarium oxysporum, Fusarium moniliforme genotype: Gibberella fujikuroi ), Fusarium oxysporumf.sp.cubense, especially Foc races 1 and 4, more especially Foc race 4.

If appropriate, isotianil can exist as a mixture of the various possible isomeric forms, especially stereoisomers, such as optical isomers.

Therefore, isotianil can be used to protect plants from or delay the attack/symptoms of the above-mentioned pathogens for a certain period of time after treatment. After the treatment of the plants with the active substance, protection is usually provided for a period of time from 1 to 30 days, preferably from 1 to 14 days. Depending on the application form, the availability of the active substance to the plants can be controlled in a targeted manner.

According to the invention, the good plant tolerance of isotianil allows the treatment of above-ground and below-ground plant parts, vegetative propagation material and soil at the concentrations required to control plant diseases.

According to the present invention, all Musaceae plants can be treated. In the context of the present invention, Musaceae plants are understood to mean all plant parts and plant populations, such as desired and undesired wild plants or crop plants (including naturally occurring crop plants). Crop plants may be Musaceae plants obtainable by traditional breeding and optimization methods or by biotechnological and recombinant methods or a combination of these methods, including transgenic Musaceae plants and including those subject to and not subject to plant breeders' rights (Plant Breeders). 'Rights) protected plant varieties such as Big Mac, Cavendish, Dwarf Cavendish, Dwarf Chinese, Enano, Caturra, Big Cavendish, Gran Enano, Grande Naine, WilliamsHybrid, Valery, Robusta, Poyo, Lacatan, Pisang masak hijau, Monte cristo, Bout rond. Plant parts are intended to mean all aerial and underground parts and organs of plants, such as herbs, pseudostems, buds, leaves, bracts, sheaths, petioles, blades, flowers and roots, examples that may be mentioned are leaves, needles , stems, stems, flowers, fruit bodies, fruits, banana hands, bunches and seeds as well as roots, tubers, rhizomes, lateral branches, suckers, secondary growth. Plant parts also include crop material and vegetative and generative propagation material, such as cuttings, tubers, rhizomes, shoots and seeds.

As already mentioned above, all Musaceae plants can be treated according to the invention. In a preferred embodiment, plant species found in the wild or obtained by conventional biological breeding methods such as crossing, meristem culture, micropropagation, somatic embryogenesis, direct organogenesis or protoplast fusion are treated and plant varieties and their parts. In another preferred embodiment, transgenic Musaceae plants and Musaceae plant varieties (genetically modified organisms) obtained by recombinant methods (if appropriate in combination with traditional methods) are treated, for example by means of Agrobacterium or embryogenic cells Transformation of particle bombardment and micropropagation. Musaceae plants include all plant parts as mentioned below.

Particular preference is given to treating according to the invention those plants of the Musaceae family which are commercially available or in use in each case. Plant varieties are understood to mean plants with novel properties ("traits") obtained by conventional breeding, by mutagenesis or by recombinant DNA techniques. They can be varieties, breeds, biotypes and genotypes.

In a preferred embodiment, the above-mentioned Musaceae plants treated according to the invention are of the Big Mack, Cavendish and Dwarf Cavendish varieties, preferably the Cavendish variety.

The treatment method according to the invention can be used for the treatment of genetically modified organisms (GMOs), such as plants or seeds. Genetically modified plants (or transgenic plants) are plants in which a heterologous gene has been stably integrated into the genome. Basically, the term "heterologous gene" refers to a gene that is provided or assembled externally to a plant, which upon introduction into the nuclear, chloroplast, or mitochondrial genome of a transformed plant, by expressing a protein or polypeptide of interest, or by down-regulating or shutting down Another gene or other genes present in the plant (eg by means of antisense technology, co-suppression technology or RNAi technology [RNA interference]) confer new or improved agronomic or other properties. Heterologous genes present in the genome are also known as transgenes. A transgene defined by its specific presence in the plant genome is called a transformation event or transgenic event.

Depending on the plant species or plant varieties, their location and their growing conditions (soil, climate, vegetative period, nutrition), the treatment according to the invention may also produce superadditive ("synergistic") effects. For example, the following effects are possible, which exceed those actually expected: lower application rates and/or broaden their spectrum of action and/or increase their efficacy of the active substances and compositions that can be used according to the invention, better plant growth , increased tolerance to high or low temperatures, increased tolerance to drought or water or soil salinity, improved flowering performance, easier harvesting, accelerated ripening, higher yield, larger fruit, higher plant height , darker leaf green, earlier flowering, better quality and/or higher nutritional value of the harvested crop, higher fruit sugar concentration, better storability and/or workability of the harvested crop.

At certain application rates, isotianil can also have a strengthening effect on plants. Therefore, they are suitable for mobilizing plant defense systems against microbial and animal pathogens. This may be one of the reasons for the increased efficacy of the combinations according to the invention, eg against fungi. Plant-strengthening (resistance-inducing) substances are also to be understood here to mean those substances or substance combinations capable of stimulating the defense system of plants in such a way that, when subsequently inoculated with microorganisms and animal pathogens, the treated plants are resistant to these microorganisms and animal pathogens. Animal pathogens exhibit a large degree of resistance. Thus, the substances according to the invention can be used to protect plants from the abovementioned pathogens for a certain period of time after treatment.

The Musaceae plants and plant varieties preferably treated according to the invention include all plants containing genetic material which confer on these plants particularly advantageous, useful traits (whether this is achieved by breeding and/or biotechnology).

It is also preferred that the Musaceae plants and plant varieties treated according to the invention are resistant to one or more biotic stress factors, i.e. these plants are resistant to animal and microbial pathogens (such as nematodes, insects, mites, phytopathogenic fungi, bacteria, virus and/or viroid) with improved defenses. Those which must preferably be mentioned in this context are Musaceae which are resistant to phytopathogenic fungi or viruses.

Musaceae plants and plant varieties which may also be treated according to the invention are those plants which are resistant to one or more abiotic stress factors. Abiotic stress conditions may include, for example, drought, low and high temperature conditions, osmotic stress, waterlogging, increased soil salinity, increased mineral exposure, ozone conditions, strong light conditions, limited nitrogen nutrient availability, limited phosphorus nutrient availability, or Avoid the shade.

Musaceae plants and plant varieties which may also be treated according to the present invention are those plants in which the vaccine or therapeutic protein is heterologously expressed. These include, for example, the hepatitis B antigen.

Musaceae plants and plant varieties that may also be treated according to the present invention are those plants characterized by improved yield characteristics. In these plants, increased yield can result from, for example, improved plant physiology, improved plant growth, and improved plant development, such as water use efficiency, water retention efficiency, improved nitrogen use, increased carbon assimilation, improved photosynthesis effect, improved seed vigor and accelerated maturation. In addition, yield can be affected by improved plant architecture (under both stressed and non-stressed conditions), including early flowering, controlled flowering to produce hybrid seeds, seedling vigour, plant size, number and distance of internodes, root growth, seed size, Fruit size, pod size, number of pods or ears, number of seeds per pod or ear, seed biomass, increased seed fill, decreased seed shedding, decreased pod shatter, and lodging resistance. Other yield-related traits include seed composition such as carbohydrate content, protein content, oil content and oil composition, nutritional value, reduction in anti-nutritional compounds, improved processability and improved storability.

Musaceae plants that can be treated according to the present invention are hybrid plants that have expressed heterosis or hybrid vigour which generally results in higher yields, higher vigour, healthier plants and better biological and Abiotic stress factors have higher resistance. Such plants are typically produced by crossing one inbred male sterile parent line (the female parent) with another inbred male fertile parent line (the male parent). Hybrid seeds are usually harvested from male sterile plants and sold to growers. Male sterile plants can sometimes (such as in maize) be produced by emasculation (ie, mechanical removal of male reproductive organs or male flowers), but, more commonly, male sterility results from genetic determinants in the plant genome . In this case, and especially when the seed is the desired product to be harvested from the hybrid plant, it is often useful to ensure that the male fertility of the hybrid plant containing the genetic determinants that cause male sterility is fully restored. This can be achieved by ensuring that the male parent has a suitable fertility restorer gene capable of restoring male fertility in hybrid plants containing genetic determinants that cause male sterility. Genetic determinants of male sterility can be located in the cytoplasm. Examples of cytoplasmic male sterility (CMS) are described, for example, in Brassica species. However, genetic determinants of male sterility can also be located in the nuclear genome. Male sterile plants can also be obtained by plant biotechnology methods such as genetic engineering. A particularly useful method of obtaining male sterile plants is described in WO 89/10396, in which, for example, a ribonuclease such as Bacillus RNase is selectively expressed in tapetum cells in the stamens. Fertility can then be restored by expressing a ribonuclease inhibitor, such as a Bacillus RNase inhibitor, in tapetum cells.

Musaceae plants or plant varieties (obtained by plant biotechnology methods such as genetic engineering) which may be treated according to the invention are herbicide tolerant plants, ie plants made tolerant to one or more given herbicides. These plants can be obtained by genetic transformation or by selection of plants containing mutations conferring tolerance to the herbicide.

Herbicide-tolerant plants are, for example, glyphosate-tolerant plants, ie plants made tolerant to the herbicide glyphosate or a salt thereof. For example, glyphosate-tolerant plants can be obtained by transforming plants with a gene encoding the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Examples of such EPSPS genes are the AroA gene (mutant CT7) of the bacterium Salmonella typhimurium, the CP4 gene of Agrobacterium sp., the gene encoding Petunia EPSPS, the tomato EPSPS gene or the gene encoding EPSPS of the genus Eleusine. It can also be a mutated EPSPS. Glyphosate-tolerant plants can also be obtained by expressing a gene encoding a glyphosate oxidoreductase. Glyphosate-tolerant plants can also be obtained by expressing a gene encoding a glyphosate acetyltransferase. Glyphosate-tolerant plants can also be obtained by selecting plants containing naturally occurring mutations in the above-mentioned genes.

Other herbicide-resistant plants are, for example, plants made tolerant to herbicides that inhibit glutamine synthase, such as bialaphos, phosphinothricin or glufosinate. Such plants can be obtained by expressing an enzyme that detoxifies the herbicide or expressing a glutamine synthase mutant that is resistant to inhibition. One such effective detoxifying enzyme is, for example, the enzyme encoding glufosinate acetyltransferase (eg, the bar or pat protein of Streptomyces sp.). Plants expressing exogenous glufosinate acetyltransferase have been described.

Other herbicide tolerant plants are also plants tolerant to herbicides that inhibit hydroxyphenylpyruvate dioxygenase (HPPD). Hydroxyphenylpyruvate dimethoxyase is an enzyme that catalyzes the reaction of converting p-hydroxyphenylpyruvate (HPP) to homogentisate. Plants tolerant to HPPD inhibitors can be transformed with a gene encoding a naturally occurring resistant HPPD enzyme or a gene encoding a mutated HPPD enzyme. Tolerance to HPPD inhibitors can also be obtained by transforming plants with genes encoding certain enzymes that form homogentisate even in the presence of inhibition of the native HPPD enzyme by the HPPD inhibitor. Tolerance of plants to HPPD inhibitors can also be improved by transforming plants with genes encoding prephenate dehydrogenase in addition to genes encoding HPPD tolerance enzymes.

Other herbicide-resistant plants are plants that are tolerant to acetolactate synthase (ALS) inhibitors. Known ALS inhibitors include, for example, sulfonylurea, imidazolidinone, triazolopyrimidines, pyrimidinyloxy(thio)benzoates, and/or sulfonylaminocarbonyltriazolinone herbicides. Different mutations in the ALS enzyme, also known as acetohydroxy acid synthase (AHAS), are known to confer tolerance to different herbicides and groups of herbicides. The generation of sulfonylurea tolerant plants and imidazolinone tolerant plants is described in International Publication WO 1996/033270. Other sulfonylurea tolerant plants and imidazolinone tolerant plants are also described, for example, in WO 2007/024782.

Other plants tolerant to imidazolinones and/or sulfonylureas can be obtained by mutagenesis, selection in cell culture in the presence of herbicides or by mutation breeding.

Musaceae plants or plant varieties (obtained by plant biotechnology methods such as genetic engineering) which may also be treated according to the invention are insect-resistant transgenic plants, ie plants which are resistant to attack by certain target insects. Such plants can be obtained by genetic transformation or by selection of plants containing mutations conferring resistance to the insects.

As used herein, an "insect-resistant transgenic plant" includes any plant that contains at least one transgene comprising a coding sequence encoding the following proteins:

1) Insecticidal crystal proteins from Bacillus thuringiensis, or insecticidal parts thereof, such as those described at: httn://www.lifesci.sussex.ac.uk/Home/Neil Crickmore /Bt/ , or a pesticidal portion thereof, such as a protein of the Cry proteins Cry1Ab, Cry1Ac, Cry1F, Cry2Ab, Cry3Ae or Cry3Bb, or a pesticidal portion thereof; or

or

3) A hybrid insecticidal protein comprising portions of two different insecticidal crystal proteins from Bacillus thuringiensis, such as a mixture of the proteins of 1) above or a mixture of proteins of 2) above, such as Cry1A produced by corn event MON98034. 105 protein (WO 2007/027777); or

4) The protein of any one of the above points 1) to 3), wherein some, especially 1 to 10 amino acids are replaced by another amino acid, thereby obtaining a higher insecticidal activity and/or amplification for the target insect species The range of target insect species affected, and/or due to changes introduced into the coding DNA during cloning or transformation, such as the Cry3Bb1 protein in maize events MON863 or MON88017, or the Cry3A protein in maize event MIR604;

5) Insecticidal secreted proteins or insecticidal parts thereof from Bacillus thuringiensis or Bacillus cereus, such as vegetative insecticidal proteins (VIPs) listed at: http://www. lifesci.sussex.ac.uk/Home/Neil_Crickmore/Bt/vip.html such as proteins from the VIP3Aa class of proteins; or

6) a secreted protein from Bacillus thuringiensis or Bacillus cereus having insecticidal activity in the presence of a second secreted protein from Bacillus thuringiensis or Bacillus cereus, such as a binary toxin consisting of VIP1A and VIP2A proteins; or

7) A mixed insecticidal protein comprising fractions of different secreted proteins from Bacillus thuringiensis or Bacillus cereus, such as a mixture of proteins of 1), or a mixture of proteins of 2) above; or

8) The protein of any of the above points 1) to 3), wherein some, especially 1 to 10, amino acids are replaced by another amino acid, thereby obtaining a higher insecticidal activity against the target insect species and/or expanding the affected range of target insect species, and/or due to changes introduced into the coding DNA during cloning or transformation (while still encoding an insecticidal protein), such as the VIP3Aa protein in cotton event COT 102.

Of course, insect-resistant transgenic plants as used herein also include any plant comprising a combination of genes encoding proteins of any of classes 1 to 8 above. In one embodiment, the insect resistant plant contains more than one transgene encoding a protein of any one of classes 1 to 8 above, to expand the range of target insect species affected, or by using Different proteins with insecticidal activity but different modes of action (eg binding to different receptor binding sites in insects) delay the development of insect resistance to plants.

Musaceae plants or plant varieties (obtained by plant biotechnology methods such as genetic engineering) which may also be treated according to the invention are tolerant to abiotic stress factors. Such plants can be obtained by genetic transformation or by selection of plants containing a mutation conferring said stress resistance. Particularly useful stress-tolerant plants include:

a. Plants containing a transgene capable of reducing the expression and/or activity of a poly(ADP-ribose) polymerase (PARP) gene in plant cells or plants;

b. Plants containing an enhanced stress tolerance transgene capable of reducing the expression and/or activity of a PARP-encoding gene in a plant or plant cell;

c. Plants containing enhanced stress tolerance transgenes encoding plant functional enzymes of the nicotinamide adenine dinucleotide salvage biosynthetic pathway, including nicotinamidase, nicotinate phosphoribosyl transfer Enzyme, nicotinic acid mononucleotide adenyl transferase, nicotinamide adenine dinucleotide synthase, or nicotinamide phosphoribosyl transferase.

Application form

According to the invention, the treatment of Musaceae plants and plant parts and propagation material with isotianil is carried out directly or by conventional treatment methods for their environment, habitat or storage space, for example by drip irrigation, spraying , atomization, spraying, broadcasting, smearing, injection.

In a particularly preferred embodiment of the present invention, isotianil or a preparation thereof is used for application to treat vegetative propagation material, or for rhizome or foliar application, or for drip irrigation, particularly preferably drip irrigation, preferably every 30 day, more preferably every 14 days, drip irrigation, preferably using 2.5 to 0.5 g aluminium triethylphosphonate/plant and 0.035 to 0.015 g IST/plant, more preferably 2.0 to 1.0 g aluminium triethylphosphonate/plant and 0.03 to 0.02 g IST/plant, even more preferably 2.0 to 1.0 g aluminium triethylphosphonate/plant and 0.03 to 0.02 g IST/plant, most preferably 1.6 g aluminium triethylphosphonate/plant and 0.024 g IST/plant.

In another particularly preferred embodiment of the present invention, isotianil or a preparation thereof is used for the application of treatment of vegetative propagation material, or for rhizome or foliar application, or for dipping application, particularly preferably for drip irrigation, preferably every 30 days, more preferably every 14 days, drip irrigation, preferably with 0.45 to 0.1 g aluminium triethylphosphonate/plant and 0.04 to 0.015 g IST/plant, more preferably 0.4 to 0.15 g aluminium triethylphosphonate/plant and 0.04 to 0.04 0.02g IST/plant, even more preferably 0.35 to 0.25g aluminium triethylphosphonate/plant and 0.035 to 0.025g IST/plant and most preferably 0.28g aluminium triethylphosphonate/plant and 0.028g IST/plant.

In an alternative embodiment of the present invention, isotianil or a formulation thereof is applied in the form of granules (for aluminium triethylphosphonate) for soil treatment.

In another particularly preferred embodiment of the invention, isotianil or its preparations are used solely as active ingredient for the treatment of vegetative propagation material, or for rhizome or foliar application, or for drip irrigation, particularly preferably drip irrigation , preferably every 30 days, more preferably every 14 days, preferably 0.035 to 0.015g IST/plant, more preferably 0.03 to 0.02g IST/plant, even more preferably 0.03 to 0.02g IST/plant and most preferably 0.024g IST/plant plant.

In another particularly preferred embodiment of the invention, isotianil or its preparations are used solely as active ingredient for the treatment of vegetative propagation material, or for rhizome or foliar application, or for drip irrigation, particularly preferably drip irrigation , preferably every 30 days, more preferably every 14 days, preferably using 0.04 to 0.015g IST/plant, more preferably 0.04 to 0.02g IST/plant, even more preferably 0.035 to 0.025g IST/plant and most preferably 0.028g IST/ plant.

In a preferred embodiment, the first treatment is performed 15 days before planting, regardless of the interval between subsequent treatments.

Depending on their respective physical and/or chemical properties, isotianil can be converted into conventional formulations such as solutions, emulsions, suspensions, powders, foams, pastes, granules, sachets, aerosols, polymers microcapsules in , as well as ULV cold and hot mists.

These formulations are prepared in a known manner, for example by mixing isotianil with extenders, ie liquid solvents, pressurized liquefied gases and/or solid carriers, optionally with the use of surfactants, ie emulsifiers and/or dispersants and/or blowing agents. If water is used as extender, it is also possible, for example, to use organic solvents as auxiliary solvents. Suitable liquid solvents are mainly: aromatic compounds, such as xylene, toluene or alkylnaphthalene; chlorinated aromatic compounds or chlorinated aliphatic hydrocarbons, such as chlorobenzene, vinyl chloride or methylene chloride; aliphatic hydrocarbons, such as cyclic Hexane or paraffins, such as mineral oil fractions; alcohols, such as butanol or glycols, and their ethers and esters; ketones, such as acetone, methyl ethyl ketone, methyl isobutyl ketone or cyclohexanone; highly polar Solvents, such as dimethylformamide and dimethylsulfoxide, and water; and mineral, animal, and vegetable oils, such as palm or other vegetable seed oils. Liquefied gaseous extenders or carriers are understood to mean those liquids which are gaseous at normal temperature and pressure, for example aerosol propellants such as halogenated hydrocarbons and butane, propane, nitrogen and carbon dioxide. Suitable solid carriers are, for example, ground natural minerals, such as kaolin, clay, talc, chalk, quartz, attapulgite, montmorillonite or diatomaceous earth; and ground synthetic minerals, such as highly dispersible silica, Alumina and Silicates. Suitable solid carriers for granules are, for example, crushed and graded natural rocks, such as calcite, pumice, marble, sepiolite, dolomite; and synthetic granules of inorganic and organic powders, as well as organic materials such as sawdust, coconut shells, Pellets of corn on the cob and tobacco stalks. Suitable emulsifiers and/or blowing agents are, for example, nonionic, cationic and anionic emulsifiers, such as polyoxyethylene fatty acid esters, polyoxyethylene fatty alcohol ethers (eg alkylarylpolyglycol ethers), alkanes sulfonates, alkyl sulfates, arylsulfonates and protein hydrolysates. Suitable dispersants are, for example, lignin sulfite waste liquor and methylcellulose.

Adhesives such as carboxymethyl cellulose, natural and synthetic polymers (eg gum arabic, polyvinyl alcohol, polyvinyl acetate) and natural phospholipids (eg cephalins and lecithins) in powder, granule or latex form can be used in the formulation ), and synthetic phospholipids. Other additives can be mineral and vegetable oils.

Colorants can be used, such as inorganic pigments, such as iron oxide, titanium oxide, Prussian blue; and organic dyes, such as alizarin, azo and metal phthalocyanine dyes; and micronutrients, such as iron salts, manganese salts, boron salts, copper salts, cobalt salts, molybdenum salts and zinc salts.

Typically, the formulation contains 5 to 95% by weight active, preferably 10 to 70% active, more preferably 15 to 30% active, and most preferably 20% active.

The control of microbial and animal pathogens by treating the vegetative propagation material of plants has been known for a long time and is the subject of continuous improvement. However, the handling of vegetative propagation material still involves a series of problems that cannot be resolved in a satisfactory manner. Therefore, it would be desirable to develop methods for the protection of vegetative propagation material and germinating plants which do not require, or at least significantly reduce, the additional application of plant protection products after planting or after plant emergence. Furthermore, it is desirable to optimize the amount of active substance used so as to provide the best possible protection for the vegetative propagation material and the germinating plant from microbial pathogens, without causing damage to the plants themselves by the active substance used. In particular, the method of handling the vegetative propagation material should also take into account the properties inherent to the transgenic plants in order to achieve the best possible protection of the vegetative propagation material and germinating plants, while keeping the application rates of plant protection products as low as possible.

Accordingly, the present invention also particularly relates to a method for protecting vegetative propagation material and germinating plants from microbial and animal pathogens by treating seeds and vegetative propagation material with the composition of the invention.

The present invention also relates to the use of a composition according to the invention for the treatment of vegetative propagation material to protect vegetative propagation material and germinating plants from microbial and animal pathogens.

One of the advantages of the present invention is that, due to the special systemic properties of the compositions according to the invention, the treatment of vegetative propagation material with these compositions not only provides protection for the vegetative propagation material itself against damage by microorganisms and animal pathogens, but also provides protection for the vegetative propagation material itself from damage by microorganisms and animal pathogens after planting. Plants grown from it provide protection. In this way, it is not necessary to treat the crop immediately at the time of sowing or shortly thereafter.

Another advantage is that, in particular, the composition of the invention can also be used on transgenic vegetative propagation material.

The compositions of the present invention are suitable for protecting the vegetative propagation material of any plant species used in agriculture, greenhouse, forestry or horticulture. In particular, it is the vegetative propagation material of the Musaceae family.

Within the scope of the present invention, the composition of the present invention is applied to the vegetative propagation material alone or in a suitable formulation. Preferably, the vegetative propagation material is treated in a sufficiently stable state so that no damage occurs during the treatment. In general, vegetative propagation material can be processed at any time between harvest and planting. Typically, the vegetative propagation material used is isolated from the plant and the cob, shell, stem, pod, hair or pulp have been removed.

In general, when handling vegetative propagation material, care must be taken to select the amount of the composition of the invention and/or other additives applied to the vegetative propagation material so that germination of the vegetative propagation material is not adversely affected, or caused by vegetative propagation Plants growing out of the material are not damaged. This has to be taken into account in particular in the case of active substances which can have phytotoxic effects at certain application rates.

The compositions of the present invention can be applied directly, in other words, free of other components and undiluted. In general, it is preferred to apply the composition in a suitable formulation to the vegetative propagation material. Suitable formulations and methods for treating seeds and vegetative propagation material are known to those skilled in the art.

The compounds that can be used according to the invention and are selected from the compounds of formula (I) can be converted into customary formulations, such as solutions, emulsions, suspensions, powders, foams and ULV formulations

These formulations are prepared in a known manner by mixing a compound selected from the group of compounds of formula (I) with customary additives, such as customary extenders as well as solvents or diluents, colorants, wetting agents, dispersants, Emulsifiers, antifoams, preservatives, secondary thickeners, adhesives, gibberellins, mineral and vegetable oils and water.

Colorants which can be present in the formulations which can be used according to the invention are all colorants customary for this purpose. In this context, sparingly water-soluble pigments and water-soluble dyes can be used. Examples which may be mentioned are the colorants known under the names Rhodamine B, C.I. Pigment Red 112 and C.I. Solvent Red 1 .

Wetting agents which can be present in the formulations which can be used according to the invention are all substances conventionally used for formulating agrochemically active substances and which promote wetting. Alkylnaphthalenesulfonates such as diisopropylnaphthalenesulfonate or diisobutylnaphthalenesulfonate may preferably be used.

Suitable dispersants and/or emulsifiers which can be present in the formulations which can be used according to the invention are all nonionic, anionic and cationic dispersants customary for the formulation of agrochemically active substances. The following can preferably be used: nonionic or anionic dispersants, or mixtures of nonionic or anionic dispersants. Suitable nonionic dispersants which may be mentioned are in particular ethylene oxide/propylene oxide block polymers, alkylphenol polyglycol ethers and tristyrylphenol polyglycol ethers, and their phosphorylated or Sulfated derivatives. Suitable anionic dispersants are in particular lignosulfonates, polyacrylates and arylsulfonate/formaldehyde condensates.

Antifoams which can be present in the formulations which can be used according to the invention are all foam inhibitor substances conventionally used for formulating agrochemical active substances. Silicone antifoams and magnesium stearate may preferably be used.

Preservatives which can be present in the formulations which can be used according to the invention are all substances which can be used for this purpose in agrochemical compositions. Examples that may be mentioned include dichlorophenol and benzyl alcohol hemiformal.

Secondary thickeners which can be present in the formulations which can be used according to the invention are all substances which can be used for this purpose in agrochemical compositions. Preferably, suitable secondary thickeners are cellulose derivatives, acrylic acid derivatives, xanthan gum, modified clays and highly dispersed silica.

Adhesives which can be present in the formulations which can be used according to the invention are all customary adhesives which can be used in mordants. Mention may preferably be made of polyvinylpyrrolidone, polyvinyl acetate, polyvinyl alcohol and sodium tylacetate.

The gibberellins which can be present in the formulations which can be used according to the invention are preferably gibberellins A1 , gibberellins A3 (gibberellic acid), gibberellins A4 and gibberellins A7. Gibberellic acid is especially preferred.

″[Chemistry of plant protection and pesticideagents]，第2卷，Springer Verlag，Berlin-Heidelberg-New York，1970，第401-412页)。Gibberellins are known (see R. Wegler"Chemie der Pflanzenschutz-und

"[Chemistry of plant protection and pesticide agents], Vol. 2, Springer Verlag, Berlin-Heidelberg-New York, 1970, pp. 401-412).

The formulations which can be used according to the invention can be used for the treatment of various types of seeds, either directly or after being previously diluted with water. Thus, the concentrate or the preparation obtainable therefrom by dilution with water can be used for dressing the seeds of Musaceae. The formulations which can be used according to the invention or their diluted formulations can also be used for the treatment of vegetative propagation material of transgenic plants. Here, additional synergistic effects can be produced in combination with substances formed by expression.

The application rates of the formulations which can be used according to the invention can vary within wide limits. The application rate depends on the content of the respective active substance in the formulation as well as on the vegetative propagation material. In general, the application rate of active substance is preferably from 0.001 to 50 g/kg of vegetative propagation material, more preferably from 0.01 to 15 g/kg of vegetative propagation material.

mixture

The compounds selected from the compounds of formula (I) can be used as such, in the form of preparations, or in combination with known fungicides, bactericides, acaricides, nematicides, herbicides, insecticides , safeners, soil improvement products or mixtures of products for reducing plant stress (eg Myconate), eg to broaden the spectrum of action or prevent the development of resistance. In many cases, this creates a synergistic effect, meaning that the efficacy of the mixture exceeds that of the individual components.

In a preferred embodiment, the present invention is a mixture of isotianil and at least one other active ingredient selected from the group consisting of aluminum triethylphosphonate, mono- and disodium salts of phosphorous acid, Monopotassium salts and dipotassium salts of phosphoric acid and monoammonium salts and diammonium salts of phosphorous acid (such as Phostrol), more preferably aluminum triethylphosphonate; wherein the ratio by weight of isotianil to mixed partner is preferably 1 to 20 to 1 to 100, more preferably 1 to 40 to 1 to 80, even more preferably 1 to 60 to 1 to 75.

In another preferred embodiment, the present invention is a mixture of isotianil with at least one other active ingredient selected from aluminum triethylphosphonate, monosodium and disodium phosphites, monopotassium and dipotassium phosphites and mono- and diammonium phosphites (such as Phostrol), more preferably aluminium triethylphosphonate; wherein the ratio by weight of isotianil to mixed partner is preferably from 1 to 1 to 1 to 50, more preferably 1 to 3 to 1 to 30, even more preferably 1 to 5 to 1 to 15.

According to the present invention, the term "mixture" means possible different combinations of at least two of the above-mentioned active substances, eg ready-to-use mixtures, tank mixes (this is understood to mean that the individual active substances are prepared by mixing and diluting before application formulation of substances) or a combination thereof (eg, a binary ready-to-use mixture of two of the above active substances is made into a tank mix by using a formulation of a third separate substance). According to the invention, it is also possible to apply the individual active substances sequentially, ie one after the other, at reasonable intervals of a few hours or days, in the case of the treatment of seeds, for example also by applying several layers containing different active substances. of active substances. Preferably, the order in which the individual active substances can be used is immaterial.

The compounds of formula (I) can be used as such, in their formulations or in the use forms prepared therefrom, such as ready-to-use solutions, suspensions, wettable powders, pastes, soluble powders, powders and granules. They are applied in a conventional manner, eg by spraying, spraying, atomizing, spreading, dusting, foaming, spreading and the like. In addition, the compounds of the formula (I) can be applied by ultra-low volume methods or the active substance preparations or the active substances themselves can be injected into the soil. It is also possible to process vegetative propagation material of plants.

When using a compound selected from the group consisting of compounds of formula (I), the application rate can vary widely, depending on the type of application. When treating plant parts, the application rate of active substance is preferably from 0.1 to 10 000 g/ha, more preferably from 10 to 1000 g/ha. When treating vegetative propagation material, the application rate of active substance is preferably 0.001 to 50 g/kg vegetative propagation material, more preferably 0.01 to 10 g/kg vegetative propagation material. When treating soil, the application rate of the active substance is preferably 0.1 to 10000 g/ha, more preferably 1 to 5000 g/ha.

The following examples are intended to illustrate the invention without imposing any limitations.

Example:

Example 1

Efficacy of isotianil and aluminum triethylphosphate against Fusarium oxysporum race 4

This example illustrates the efficacy of a composition containing isotianil against Fusarium oxysporum race 4 in Cavendish-type bananas.

The trial was conducted using a randomized complete block design (RCBD) with 4 treatments (10 plants/treatment) as follows:

a) untreated,

b) Aliette 80WG 2g/plant (FEA 1.6g active ingredient/plant),

c) Isotianil SC 200g/L 0.12mL/plant (IST 0.024g active ingredient/plant), and

d) Aliette 80WG+isothiazide SC200-2g+0.12mL/plant (1.6+0.024g active ingredient/plant).

Apply monthly by soil drenching.

result

Fusarium infection affected by different fungicide applications

Trial 1: Preliminary trial results indicated that monthly prophylactic application of 2 grams of Aliette + 0.12 mL of isotianil per plant in the form of soil drenching provided 90% protection against Fusarium infection. Infection was controlled at 30% and 50% when 0.12 mL of isotianil and 2 g of Aliette/plant were applied alone. In this case, the synergy between the two compounds can be determined (Colby's formula - efficacy calculated by Abbott: 65%). In addition, symptom onset was delayed on treated plants by 22 days (Aliette and isotianil alone) and 155 days (Aliette + isotianil) compared to the untreated control group (UTC).

Example 2

Efficacy of isotianil and isotianil + aluminum triethylphosphonate against Fusarium oxysporum race 1

This example illustrates the efficacy of a composition comprising isotianil against Fusarium oxysporum race 1 in the banana variety Big Mac.

The trial was conducted using a randomized complete block design (RCBD) with 3 blocks with 8 replicates each - all treated as follows:

e) not processed,

f) Aliette 80WG 2g/plant (FEA 1.6g active ingredient/plant),

g) Isotianil SC 200g/L 0.12mL/plant (IST 0.024g active ingredient/plant). and

h) Aliette 80WG+Isothiazide SC200-2g+0.12mL/plant (1.8+0.024g active ingredient/plant).

Application was performed as follows: soil soaking was started in the nursery 15 days before planting and continued at planting time, then monthly (application at the base of the plant and around 40 cm). The evaluation tests were the percentage of infected plants and the severity of tree trunk infection (severity scale 0=asymptomatic to 4=plant death) at the end of the test period.

Results of a trial in Costa Rica in 2015/2016

Trial 2: Final trial results showed that monthly prophylactic application of 2 g of Aliette + 0.12 mL of isothiazide/plant in soil soaking provided 100% protection from Fusarium infection 90 days after planting . Infections were controlled at 43% and 27% when 0.12 mL of isotianil and 2 g of Aliette/plant were applied alone. In this case, the synergy between the two compounds can be determined (Colby's formula - efficacy calculated by Abbott: 58%). In addition, despite the lower persistence of the single compounds, the mixtures exhibited higher levels of control and reduced infection severity at the highest stems 180 days after planting.

Example 3

Room temperature test-Efficacy of isotianil + aluminum triethylphosphonate against Fusarium oxysporum race 4

This example illustrates the efficacy of a composition containing isotianil against Fusarium oxysporum race 4 on the banana plant Cavendish cultivar GrandeNaine.

Experiments were carried out in the greenhouse with 30 plants per treatment (10 plants each in 3 replicates). Transplant two-month-old banana plants into infected soil. The compound was infiltrated (protective administration) 6 days before transplantation. The second administration was performed 4 weeks after the first administration. Administration takes place in the following manner:

j) Aliette 80WG 2g/plant (FEA 1.6g active ingredient/plant), and

k) Aliette 80WG + Isothiazide SC200-2g + 0.12mL/plant (1.8+0.024g active ingredient/plant) compared to i) untreated contaminated plants.

Six weeks after inoculation, plants were scored on an isometric scale for fading, a typical symptom of Fusarium oxysporum. Scale 0 = asymptomatic to 6 = complete plant necrosis

result

Trial 3: Final trial results at 6 weeks post-vaccination showed significant differences in the following protective protection:

i) untreated contaminated (average of 3 replicates = 6),

j) Aliette 2g/plant (average of 13 replicates for T1C = 5.3), and

k) Aliette + Isotianil 2 g + 0.12 mL/plant (Trt T4C 13 replicate mean = score 1.7). Where Aliette was less effective, the mixture of aluminum triethylphosphonate + isotianil provided good protection against Foc under these very severe infection conditions, suggesting that it would be advantageous to mix it with isotianil.

Example 4 - Soil Application - 2 Ratio Test Applications

Isothiazide and aluminum triethylphosphonate (ratio 60:1 and ratio 10:1) applied by saturating against Fusarium oxysporum Efficacy of bacterial race 4

This example illustrates the efficacy of two compositions containing aluminum triethylphosphonate and isotianil applied in a flooded fashion against Fusarium oxysporum race 4 in banana plants.

The trial was conducted in a randomized complete block design (RCBD) plantation of the Cavendish variety (3 replicates/treatment - a total of 20 plants/plot evaluated), including untreated, aluminum triethylphosphonate + iso Thiapyrad (SP102000028595WG77% - ratio 10:1) - 0.4g/plant (0.3+0.03g active ingredient/plant), aluminum triethylphosphonate (Fosetyl) + isotianil (SP102000033663 WG76.5% - ratio 60) : 1)-0.4g/plant (0.3+0.005g/plant). The ratios of the 2 formulations were calculated to yield similar amounts of aluminum triethylphosphonate and variable amounts of isotianil. All FEA+IST applications were carried out as immersion applications, once a month, with 500 ml of water per plant.

Preliminary trial results at 2 sites indicated that the use of FEA+IST in a drenched application method significantly delayed the development of F. oxysporum race 4 at both 10:1 and 60:1 ratios. This delay in disease development translates into retention of production potential in the treated plots.

Results of two trials:

Example 5 - Foliar application - 2 ratios tested

Isotianil and aluminum triethylphosphonate (ratio 60:1 and ratio 10:1) applied as a foliar spray against Fusarium oxysporum Efficacy of bacterial race 4

This example illustrates the efficacy of two compositions containing aluminum triethylphosphonate and isotianil applied as a foliar spray against Fusarium oxysporum race 4 in banana plants.

The trial was conducted in a randomized complete block design (RCBD) plantation of the Cavendish variety (3 replicates/treatment - a total of 20 plants/plot evaluated), including untreated, aluminum triethylphosphonate + iso Thiapyrad (SP102000028595WG77%-ratio 10:1)-2g/plant (1.4+0.14g active ingredient/plant), aluminum triethylphosphonate (Fosetyl)+isothiazide (SP102000033663 WG76.5%-ratio 60: 1)-2g/plant (1.5+0.025g/plant). All FEA+IST applications were performed as foliar applications once a month with 50 ml of water per plant.

Preliminary test results at 2 sites showed that the use of FEA+IST as a foliar spray significantly delayed the development of F. oxysporum race 4 at both 10:1 and 60:1 ratios. The delay in the development of this disease means that the production potential in the treated plots is preserved.

Results of two trials:

Conclusion on protecting banana plantations from Fusarium oxysporum, which causes fusarium wilt

The five examples documented demonstrate the advantages of using isotianil-based compounds to limit the development of F. oxysporum race 4 in banana plants. A mixture of isotianil and aluminium triethylphosphonate showed even better protection in field plantations. The 2 ratios tested in saturating application and foliar spray showed a significant reduction in disease infection and under certain conditions would delay the development of Panama disease in banana plantations. For producers, this advantage means better plantation survival with fewer infected plants and higher yields.

Claims (13)

1. Use of isotianil (formula (I)) for preventing and treating Panama disease in Musaceae plants

It is characterized in that isotianil is used in combination with aluminum triethylphosphonate in a ratio of 1:60 to 1:75 or in a ratio of 1:5 to 1:15, in % by weight. 2. Use of isotianil according to claim 1 for controlling fungi of the genus Fusarium oxysporum in Musaceae plants. 3. The purposes of the isotianil according to claim 1 or 2, which is used for controlling the fungus of Fusarium oxysporum Cuban-specialized type in Musaceae plants. 4. The purposes of the isotianil according to claim 1 or 2, it is used for preventing and treating the fungus of Fusarium oxysporum Cuban specialization type race 1 in Musaceae plants. 5. The purposes of isotianil according to claim 1 or 2, which is used for controlling the fungus of Fusarium oxysporum Cuban specialization race 4 in Musaceae plants. 6. The use of isotianil according to claim 1 or 2, wherein the Musaceae plant is selected from Cavendish or Big Mac varieties. 7. A method for controlling Panama disease in Musaceae plants, characterized in that isotianil (I) and aluminum triethylphosphonate are used in a ratio of 1:60 to 1:75 or in a ratio of 1:5 to 1:15 The ratio of the combined treatment Musaceae plants, in % by weight. 8. The method of claim 7, wherein the first treatment is performed 15 days before planting. 9. The method according to claim 7 or 8, characterized in that the treatments are carried out at intervals of 30 days. 10. The method of claim 7 or 8, wherein the treatments are performed at 14-day intervals. 11. The method according to claim 7 or 8, wherein the amount of active ingredient per treatment per plant is 2.5 to 0.5 g aluminium triethylphosphonate and 0.035 to 0.015 g isotianil, or two combination of . 12. The method according to claim 7 or 8, wherein the amount of active ingredient per treatment per plant is 0.45 to 0.1 g aluminum triethylphosphonate and 0.04 to 0.015 g isotianil, or both. combination of . 13. The method of claim 7 or 8, wherein the treatment is a drip irrigation application.