CN111227229A

CN111227229A - Plant source nutrition powder and preparation method and edible method thereof

Info

Publication number: CN111227229A
Application number: CN202010029153.4A
Authority: CN
Inventors: 刘润钊
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-01-13
Filing date: 2020-01-13
Publication date: 2020-06-05

Abstract

The invention provides a plant source nutrition powder and a preparation method and an edible method thereof. A plant source nutrition powder comprises the following raw materials in parts by weight: 3-10 parts of soybeans, 3-8 parts of Chinese yam, 2-7 parts of saussurea involucrate seeds, 8-15 parts of oats, 5-10 parts of almonds, 0-1.5 parts of white sesame seeds, 0-1.5 parts of walnuts, 0-20 parts of coix seeds, 0-20 parts of poria cocos and 50-80 parts of coconut powder. The raw material combination can provide balanced and comprehensive nutrition level for users, and is beneficial to converting healthy life style, thereby regulating and reducing the contents of blood sugar, blood fat and serum cholesterol, preventing the cholesterol from depositing on the inner wall of blood vessels, removing the deposits, reducing the blood viscosity and promoting the blood circulation.

Description

Plant source nutrition powder and preparation method and edible method thereof

Technical Field

The invention belongs to the field of functional foods, and particularly relates to plant source nutrition powder and a preparation method and an edible method thereof.

Background

At present, the dietary structure of people is greatly changed, sub-health people are increased year by year, and particularly, three-high patients are remarkably increased due to the fact that the life rhythm of developed regions is accelerated and the working pressure is increased. More and more people pay attention to health care and pay attention to diet, so that the research on functional ingredients of food and the development of functional food become hot spots of food research at home and abroad aiming at the specific requirements of specific people, and the method has wide market prospect.

Disclosure of Invention

The invention aims to provide plant source nutrition powder, a preparation method and an eating method thereof, so as to improve the blood pressure, blood fat and blood sugar levels of people eating the powder through functional food.

According to one aspect of the invention, the plant source nutrition powder comprises the following raw materials in parts by weight: 3-10 parts of soybeans, 3-8 parts of Chinese yam, 2-7 parts of saussurea involucrate seeds, 8-15 parts of oats, 5-10 parts of almonds, 0-1.5 parts of white sesame seeds, 0-1.5 parts of walnuts, 0-20 parts of coix seeds, 0-20 parts of poria cocos and 50-80 parts of coconut powder.

Preferably, the raw materials comprise, by mass: 3.5-6 parts of soybeans, 4-7 parts of Chinese yam, 2.5-5 parts of saussurea involucrate seeds, 10-15 parts of oats, 6-9 parts of almonds, 1-8 parts of coix seeds, 1-5 parts of poria cocos and 60-70 parts of coconut powder.

Preferably, the raw materials comprise, by mass: 5 parts of soybeans, 5 parts of Chinese yam, 4 parts of snow lotus seeds, 12 parts of oat, 8 parts of almonds, 1 part of white sesame seeds, 1 part of walnuts, 3 parts of coix seeds, 2 parts of poria cocos and 64 parts of coconut powder.

Preferably, the raw materials also comprise 0.5 to 3 parts of trehalose by weight.

Preferably, the raw material comprises 1 part of trehalose by mass.

According to another aspect of the present invention, there is provided a method for preparing the plant source nutrition powder, which comprises the following steps: steam explosion pretreatment: performing steam explosion pretreatment on the raw materials by adopting a steam explosion method to obtain a steam explosion product; and (3) drying: drying the steam explosion product; a material mixing step: and uniformly mixing the dried materials to obtain a finished product.

Preferably, the specific parameters of the steam explosion pretreatment step are set as follows: setting the steam explosion pressure at 0.5-3 MPa, and maintaining for 15-45 s.

According to another aspect of the present invention, there is provided a method for eating the plant source nutrition powder, which is characterized in that: according to the plant source nutrition powder: milk 1: 3-10, and adding the plant source nutrition powder into the milk for administration.

The raw materials are combined to form the raw material components of the plant source nutrition powder, and the raw material combination can provide balanced and comprehensive nutrition level for eaters, particularly, various active substances such as lecithin, isoflavone, Chinese yam mucilage, β -glucan, antioxidant peptide, ACE (angiotensin converting enzyme) inhibitory peptide, platelet aggregation activity inhibitory peptide, antihypertensive peptide, lactoferrin, immunoglobulin, metallothionein, brown pigment, enzyme protein, collagen, alcohol soluble protein and soybean protein isolate contained in the raw materials can be converted into a healthy life style through various in vivo action mechanisms, so that the blood sugar, blood fat and serum cholesterol content can be adjusted, the deposition of cholesterol on the inner wall of a blood vessel is prevented, the viscosity of the cholesterol is eliminated, and the blood circulation function of the blood is reduced.

The method provided by the invention is adopted to process the raw materials, so that the effective active ingredients in the raw materials can be effectively released, or the nutrient substances in the raw materials are converted to a form more beneficial to the absorption of a human body, and the finally prepared plant source nutrient powder can help to regulate the blood sugar, the blood fat and the blood pressure of a user, is beneficial to keeping the blood sugar, the blood fat and the blood pressure of the user in a normal level range, and promotes the cardiovascular and cerebrovascular of the user to be in a healthy state.

The plant source nutrition powder provided by the invention does not contain any additives such as hormone, antibiotics, preservatives and the like, has fragrant and smooth taste, does not cause excessive internal heat, can be taken by being mixed with milk or honey, and can also be used as a health maintenance meal replacement bag. The milk is a nutrient substance containing rich nutrient components, however, in real life, many children and children can cause the phenomena of constipation, hemorrhoids, pharyngitis, fever and the like caused by drinking the milk, the milk is added into the plant source nutrient powder provided by the invention to prepare the plant milk, and the plant milk is used as a daily diet regulating food, so that the excessive ingestion of fish, meat, eggs and milk of a user can be effectively relieved, and various chronic diseases caused by the excessive fish, meat, eggs and milk can be effectively reduced. The proper use of the 'plant milk' can effectively reduce the intake of chemical substances such as food additives, antiseptics, antibiotics, hormones and the like in a human body, reduce the dependence on fish, egg and milk, reduce the adverse effect of regulating blood fat, blood sugar and cholesterol on the human body, effectively prevent various acute and chronic diseases, can shape and beautify the skin, and supplement various trace elements, plant protein, amino acid, fatty acid and other nutrient substances required by the human body, and is a faithful partner for the health of the whole people in modern elegant life.

Drawings

FIG. 1 is a graph showing the change in SBP of the experimental mouse of example 3;

FIG. 2 is a graph showing DBP changes in the experimental mice of example 3.

Detailed Description

In order to make the technical solutions of the present invention better understood by those skilled in the art, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.

Example 1

In this example, 5 plant source nutrition powders were prepared by weighing the required raw materials according to the raw material amounts listed in table 1.

TABLE 1 raw Material composition of the plant-derived nutrient powder prepared in example 1

The preparation method of the plant source nutrition powder A, the plant source nutrition powder B, the plant source nutrition powder C and the plant source nutrition powder D comprises the following steps:

weighing the required raw materials according to the amount shown in the table 1, mixing the raw materials, cutting into powder, further uniformly mixing the crushed raw materials, and sieving by a 40-mesh sieve to obtain a finished product.

The main equipment required for preparing the plant source nutrition powder E comprises the following components: QBS-80 supersonic ejection type air blasting machine (Heliandao biological energy Co., Ltd.), experimental spray dryer SP-1500 (Shanghai Shunji). The preparation method of the plant source nutrition powder E comprises the following steps:

weighing the required raw materials according to the amount shown in table 1, mixing the raw materials, cutting the raw materials into pieces, putting the crushed raw materials into a reaction chamber of a steam explosion machine, setting the steam explosion pressure to be 2MPa and maintaining the pressure for 30 seconds to obtain a steam explosion product, drying the steam explosion product by using a spray dryer to obtain a semi-finished product, cutting the semi-finished product into powder, further uniformly mixing the powder, and sieving the powder by using a 40-mesh sieve to obtain the plant source nutrition powder E.

Example 2

Blood lipid lowering experiment

1. Experimental construction mode

Hyperlipidemia is a common disorder of lipid metabolism, and can be promoted by high-fat and high-cholesterol diet, and hyperlipidemia is a main cause of atherosclerosis. In this example, an experimental group was set up using 5 plant-derived nutritional powders prepared in example 1 as reference products, a control group was set up, and a hyperlipidemic rat model was constructed using high fat emulsion gavage SD (Sprague-dawley, SD) rats. The lipid metabolism of rats is observed, and serum TG, TC, LDL-C and HDL-C are detected simultaneously, so as to preliminarily discuss the protective effect of each reference product on blood vessels.

2. Main experimental materials

Lard, commercially available; cholesterol, shanghai xin tide biotechnology, ltd; propylthiouracil, Shanghai Korea Daihui pharmaceutical Co., Ltd; sodium cholate, shanghai source leaf biotechnology limited; simvastatin, a limited Tianfang pharmaceutical industry company; four blood lipid detection kits (TC/TG/LDL-C/HDL-C) and NOS detection kits; preparing a high-fat emulsion (Liulijuan, Zhouchui. an experimental study for establishing a rat model of hyperlipidemia and hypertension [ J ]. J. Med. in lake south, 2010,26(6): 111-112.); the other reagents are analytically pure.

3. Main equipment

Bio Tek Eon^TMMicroplate spectrophotometer, Biotek Instruments, Ins, usa;

ALLEGRAX-12R Universal desk top refrigerated centrifuge, Beckman Coulter, USA;

FRESCO21 Mini desk refrigerated centrifuge, Theromo Scientific, USA;

free Zone 12plus vacuum freeze dryer, Labconco, usa;

SJM-DGN-02 multifunctional membrane separation equipment, Hefeijie Membrane engineering, LLC.

4. Laboratory animal management

Experimental animals SPF grade male SD rats, 6 weeks old, 120 + -20 g in body weight. Raising temperature: 25 ± 2 ℃, humidity: 40-70%, 12h light and shade alternate illumination, free diet and water intake. All experiments were reviewed and approved by the ethics committee for laboratory animals and performed according to the operating guidelines.

After 7 days of adaptive feeding, the mice were divided into a blank group and a high fat emulsion group, the blank group was re-fed with physiological saline every 1mL/100g body per day, and the high fat emulsion group was re-fed with high fat emulsion every 1mL/100g body for 1 time/d for 60 days, while normally supplying drinking water and basal diet. After the last 1 gastric lavage, the animals in each group were fasted for 12h without water supply. The infraorbital venous plexus was bled, serum was isolated, and serum TC, LDL-C and HDL-C levels were determined to see if the model was successful. And randomly selecting 48 high-fat model groups of experimental mice successfully modeled, randomly dividing the experimental mice into 6 groups, and respectively numbering the groups into an experiment I group, an experiment II group, an experiment III group, an experiment IV group, an experiment V group and a control II group, wherein each group comprises 8 mice, and each experimental mouse is a duplicate. From the blank group, 8 mice were randomly selected as control group I mice, one replicate for each mouse.

5. Preparation of feeding liquid

The 5 plant source nutrition powders prepared in the example 1 are used as reference products, each group of reference products (powder) are weighed according to the dosage of 105mg/kg, the reference products are mixed with boiled water to prepare feeding liquid, the feeding liquid is used for feeding experimental mice of an experimental group, and the experimental mice of a control group I and a control group II are fed with normal saline. Groups of mice and corresponding gavage liquid are shown in table 1.

TABLE 1 grouping of blood fat-reducing experimental animals and ingredients of gastric lavage liquid

Group of	Gastric lavage liquid material	Group of	Gastric lavage liquid material
				Experiment I group	Plant source nutrition powder A	Control group I	Physiological saline
Experiment II group	Plant source nutrition powder B	Control group II	Physiological saline
				Experiment group III	Plant source nutrition powder C
Experimental group IV	Plant source nutrition powder D
				Experiment group V	Plant source nutrition powder E

6. Index acquisition mode

Serum preparation: after the last gastric lavage of each group of animals, fasting for 12h without water prohibition, carrying out abdominal anesthesia by using 10% chloral hydrate according to 400mg/kg of body weight, collecting about 5mL of whole blood from abdominal aorta, standing at room temperature, naturally coagulating the blood, centrifuging (3500rpm, 10min, 4 ℃), taking yellow transparent supernatant fluid as serum, and subpackaging the serum into a plurality of 1.5m L Eppendorf (EP) tubes for later use at-20 ℃. The TC, LDL-C and HDL-C of serum are measured by using a microplate spectrophotometer and a scientific research kit, and the specific operation method refers to the kit specification.

With reference to the method provided in the prior art (Feng L J, Yu C H, Ying K J, et al. Hyperpolipidemic and systemic effects of total flavanoids of Perilla fractions with induced by high-fat stream [ J ]. Food research International,2011,44(1):404- > 409 ]), the Atherosclerosis Index (AI) is calculated according to the following formula:

7. results of the experiment

Hyperlipidemia is characterized by abnormal lipid levels caused by elevated TC and LDL-C levels and lowered HDL-C levels in the blood. Compared with the control group I, the TC and LDL-C levels of the experimental mice of the control group II and the experimental groups I-V are obviously higher, while the HDL-C level is obviously lower before the test products are fed, so that the successful modeling of the experimental mice of the high-fat emulsion group is proved. Table 2 shows the change of serum index before and after the experiment of feeding the test products. As can be seen from Table 2, the serum index levels of the groups I-V of the test products fed by the feeding were improved, wherein the serum index of the group IV of the test products was closest to the normal level (group II of the control products), and the serum index of the group II of the control products was further developed in the direction deviating from the normal level. The AI of the experimental groups I-V is obviously reduced, and the plant source nutrition powder prepared in the example 1 can improve the adverse condition of atherosclerosis of experimental mice and has a certain prevention effect on atherosclerosis.

According to the experimental results of this example, 5 plant-derived nutrition powders prepared in example 1 can effectively reduce the blood lipid level of the experimental mouse, and the magnitude sequence of the blood lipid reducing effect is as follows: the plant source nutrition powder E, the plant source nutrition powder B, the plant source nutrition powder D, the plant source nutrition powder C and the plant source nutrition powder A are all mixed.

TABLE 2 serum indices before (day 0) and after (day 30) the feeding experiment

(unit: mmol/L)

Example 3

Blood pressure lowering experiment

1. Animal management mode

Experiments were performed using 14-17 week old male SHR rats weighing 280 ± 20g, feeding temperature: 25 ± 2 ℃, humidity: 40-70%, 12h light and shade alternate illumination, and free intake and drinking. All experiments were approved and approved by the laboratory animal ethics committee and performed according to the operating guidelines.

2. Principal devices or materials

NO rat Elisa kit, tokyo senega biotechnology ltd;

ALC-NIBP non-invasive blood pressure measurement and analysis system, Shanghai Oercote Biotech limited;

bio Tek Eon TM microplate spectrophotometer, Biotek Instruments, Ins, USA;

tissue grinder with medium and high flux of Tissue Lyser II, QIAGEN, Germany;

ALLEGRAX-12R Universal desk top refrigerated centrifuge, Beckman Coulter, USA;

FRESCO21 Mini desk refrigerated centrifuge, Theromo Scientific, USA;

free Zone 12plus vacuum freeze dryer, Labconco, usa.

3. Preparation of feeding liquid

The 5 plant-derived nutrition powders prepared in example 1 were used as reference products, each group of reference products (powder) was weighed according to a dosage of 105mg/kg, and the reference products were mixed with boiled water to prepare feeding solutions, and the feeding solutions were fed to the experimental rats of the experimental group, and the experimental rats of the control group were fed with physiological saline. Groups of mice and corresponding gavage liquid are shown in table 3.

TABLE 3 grouping of hypotensive experimental animals and ingredients of gavage liquid

Group of	Gastric lavage liquid material	Group of	Gastric lavage liquid material
				Experiment I group	Plant source nutrition powder A	Experimental group IV	Plant source nutrition powder D
Experiment II group	Plant source nutrition powder B	Experiment group V	Plant source nutrition powder E
				Experiment group III	Plant source nutrition powder C	Control group	Physiological saline

4. Data acquisition mode

A42-day Blood Pressure lowering effect test was conducted using a mouse having a Systolic Blood Pressure (SBP) of 170mm Hg or more. After 10 days of adaptive training in SHR, gavage experiments were performed and the animal groups and doses are shown in Table 3. The gavage time was continued for 6 weeks, SBP and Diastolic Blood Pressure (DBP) were measured once a week, and the effect of each test substance on the Blood Pressure of SHR rats was observed.

5. Results of the experiment

The test results of SBP and DBP are shown in FIG. 1 and FIG. 2, respectively, and it can be seen from the figure that SBP and DBP of the control group always maintain higher levels, relatively speaking, SBP and DBP levels corresponding to the experimental groups I-V are all in a descending trend, and the magnitude order of the pressure-reducing effect is as follows: the plant source nutrition powder A, the plant source nutrition powder E, the plant source nutrition powder B, the plant source nutrition powder D and the plant source nutrition powder C are sequentially mixed. Compared with the experimental mouse fed with the plant source nutrition powder A, the experimental mouse fed with the plant source nutrition powder B, the plant source nutrition powder C, the plant source nutrition powder D and the plant source nutrition powder E respectively has a relatively stable trend of decreasing the blood sugar level, so that the experimental mouse is more beneficial to the adaptation of a body, and when the experiment is carried out for 42 days, the blood pressure of the experimental mouse fed with the plant source nutrition powder E is closest to a normal value.

Example 4

Blood sugar lowering experiment

1. Experimental construction mode

After 7 days of adaptive feeding, the rats are divided into a blank group and a hyperglycemic group after fasting for 16 hours without water prohibition, and are injected with 2% alloxan solution in the abdominal cavity, the first intraperitoneal injection dosage is 150mg/kg of 2% alloxan solution, and the second intraperitoneal injection dosage is 120mg/kg of 2% alloxan solution. And 25% glucose was administered 10mL/kg after injection and gavage, respectively. Blood was collected 7 days after injection, and the blood glucose concentration was selected as the success of the diabetic rat model, and the same amount of physiological saline was injected daily to the rats in the blank group.

48 experimental mice in a hyperglycemia group which are successfully modeled are randomly selected, are randomly divided into 6 groups and are respectively numbered as an experiment I group, an experiment II group, an experiment III group, an experiment IV group, an experiment V group and a control II group, 8 experimental mice in each group are repeated. From the blank group, 8 mice were randomly selected as control group I mice, one replicate for each mouse.

2. Data acquisition mode

Blood was collected on

days

0, 7 and 14 after feeding the test products to measure fasting blood glucose, rats in each group were fasted without water supply during the blood collection day, and blood was collected by tail-cutting method and blood glucose value was measured by glucometer according to the instruction.

3. Results of the experiment

The fasting blood glucose test results of the experimental mice are shown in table 4, and before the test products are fed, the blood glucose values of the experimental mice in the experimental groups I-V and the experimental mice in the control group II are obviously higher than those of the control group I, so that the successful modeling of the experimental mice in the experimental groups I-V and the hyperglycemic experimental mice in the control group II is shown. After the feeding experiment is carried out, the blood sugar values of the experimental rats of the experimental IV group and the experimental V group are obviously reduced on the 7 th day, the blood sugar values of the experimental rats of the experimental I-V groups are reduced to a certain extent on the 14 th day, and the blood sugar values of the experimental rats of the experimental IV group and the experimental V group are close to the normal blood sugar level.

TABLE 4 fasting blood glucose test results (unit: mmol/L) of the experimental mouse of this example

Group of	Day 0	Day 7	Day 14
				Control group I	5.4	5.5	5.4
Control group II	25.6	25.8	26.4
				Experiment I group	25.9	19.8	8.4
Experiment II group	26.1	22.8	13.3
				Experiment group III	25.8	20.7	9.3
Experimental group IV	25.9	18.4	6.9
				Experiment group V	26.0	17.2	5.6

According to the experimental results of this example, 5 plant-derived nutrition powders prepared in example 1 can effectively reduce the blood sugar level of laboratory mice, and the order of the blood sugar reducing effect is as follows: the plant source nutrition powder E, the plant source nutrition powder D, the plant source nutrition powder A, the plant source nutrition powder C and the plant source nutrition powder B are sequentially mixed.

Although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes, modifications, and equivalents may be made therein without departing from the spirit and scope of the invention.

Claims

1. The plant source nutrition powder is characterized by comprising the following raw materials in parts by weight: 3-10 parts of soybeans, 3-8 parts of Chinese yam, 2-7 parts of saussurea involucrate seeds, 8-15 parts of oats, 5-10 parts of almonds, 0-1.5 parts of white sesame seeds, 0-1.5 parts of walnuts, 0-20 parts of coix seeds, 0-20 parts of poria cocos and 50-80 parts of coconut powder.

2. The plant nutrition powder as claimed in claim 1, wherein the raw materials comprise, in parts by mass: 3.5-6 parts of soybeans, 4-7 parts of Chinese yam, 2.5-5 parts of saussurea involucrate seeds, 10-15 parts of oats, 6-9 parts of almonds, 1-8 parts of coix seeds, 1-5 parts of poria cocos and 60-70 parts of coconut powder.

3. The plant source nutrition powder of claim 1, wherein the raw materials comprise, in parts by mass: 5 parts of soybeans, 5 parts of Chinese yam, 4 parts of snow lotus seeds, 12 parts of oats, 8 parts of almonds, 1 part of white sesames, 1 part of walnuts, 3 parts of coix seeds, 2 parts of poria cocos and 64 parts of coconut powder.

4. The plant-derived nutritional powder of claim 1, wherein: the raw materials also comprise 0.5-3 parts of trehalose by weight.

5. The plant-derived nutritional powder of claim 4, wherein: the raw materials comprise 1 part of trehalose by weight.

6. A method of producing a plant derived nutritional powder according to any one of claims 1 to 5, comprising the steps of, in order:

steam explosion pretreatment: performing steam explosion pretreatment on the raw materials by adopting a steam explosion method to obtain a steam explosion product;

and (3) drying: drying the steam explosion product;

a material mixing step: and uniformly mixing the dried materials to obtain a finished product.

7. The preparation method of the plant source nutrition powder as claimed in claim 6, wherein the specific parameters of the steam explosion pretreatment step are as follows: setting the steam explosion pressure at 0.5-3 MPa, and maintaining for 15-45 s.

8. A method for eating the plant source nutrition powder as claimed in any one of claims 1 to 5, which is characterized in that: according to the plant source nutrition powder: milk 1: 3-10, and the plant source nutrition powder is added into milk for administration.