CN111149773B - Drosophila resistance strain screening system - Google Patents
Drosophila resistance strain screening system Download PDFInfo
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- CN111149773B CN111149773B CN202010097736.0A CN202010097736A CN111149773B CN 111149773 B CN111149773 B CN 111149773B CN 202010097736 A CN202010097736 A CN 202010097736A CN 111149773 B CN111149773 B CN 111149773B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/60—New or modified breeds of invertebrates
- A01K67/61—Genetically modified invertebrates, e.g. transgenic or polyploid
- A01K67/65—Genetically modified arthropods
- A01K67/68—Genetically modified insects
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Abstract
本发明提供一种果蝇抗性品系筛选系统，属于昆虫研究领域，以提供一种能筛选具有抗药性果蝇的系统。包括若干个果蝇培养管，各个果蝇培养管均配置有管塞，管塞下部与果蝇培养管上部连接，上一个管塞上部与导光管前端连接，导光管末端与下一个果蝇培养管上部连接，除第一个果蝇培养管之外的果蝇培养管侧壁上部均设有黄绿光源，导光管内部安装有果蝇引导器，导光管后端连接有果蝇引导器，果蝇引导器为圆台形并为单向导光材料，果蝇引导器的第一圆面的直径大于第二圆面的直径，导光管内部的果蝇引导器的第一圆面位于其第二圆面之前，黄绿光源所在的圆柱形管的开口正对导光管末端所连接的果蝇引导器的第二圆面，各个黄绿光源均与控制装置和电源连接。
The invention provides a Drosophila resistance strain screening system, which belongs to the field of insect research and provides a system capable of screening Drosophila with drug resistance. Including several fruit fly culture tubes, each fruit fly culture tube is equipped with a tube plug, the lower part of the tube plug is connected with the upper part of the fruit fly culture tube, the upper part of the previous tube plug is connected with the front end of the light pipe, and the end of the light pipe is connected with the next fruit fly culture tube. The upper part of the fly culture tube is connected, and the upper part of the side wall of the fruit fly culture tube except the first fruit fly culture tube is provided with a yellow-green light source. The fly guide, the fruit fly guide is a truncated cone and is a unidirectional light guide material, the diameter of the first circular surface of the fruit fly guide is larger than the diameter of the second circular surface, and the first circle of the fruit fly guide inside the light guide The opening of the cylindrical tube where the yellow-green light source is located is facing the second circular surface of the fruit fly guide connected to the end of the light guide, and each yellow-green light source is connected to the control device and the power supply.
Description
Technical Field
The invention relates to the technical field of insect research, in particular to a drosophila resistant strain screening system.
Background
Screening for resistant lines is important for entomology research. Fruit flies are an important model insect with many advantages not found in non-model insects. The directed screening of the strain of the drosophila melanogaster which can resist a certain pesticide or chemical agent is particularly important for the deep research of the detoxification mechanism and the metabolic mechanism of the drosophila melanogaster. For this reason, it is necessary to provide a system capable of performing line screening for resistance to Drosophila.
Disclosure of Invention
The invention aims to provide a drosophila resistance strain screening system capable of screening drosophila with certain drug resistance.
In order to solve the technical problems, the invention adopts the technical scheme that:
a fruit fly resistant strain screening system comprises a plurality of fruit fly culture tubes, wherein a culture medium is placed into each fruit fly culture tube which is sequentially arranged from left to right, a pesticide is placed into the culture medium, the pesticide amount placed into each fruit fly culture tube which is sequentially arranged from left to right is gradually increased, the fruit fly culture tubes are cylindrical, each fruit fly culture tube is provided with a tube plug which is in an inverted funnel shape, the lower part of the tube plug is connected with the upper part of the fruit fly culture tube, the upper part of the previous tube plug is connected with the front end of a light guide tube, the tail end of the light guide tube is connected with the upper part of the next fruit fly culture tube, yellow green light sources are arranged on the upper parts of the side walls of other fruit fly culture tubes except the first fruit fly culture tube, the yellow green light sources are arranged in the cylindrical tube with an opening at one end, a plurality of fruit fly guides are arranged in the light guide tube, and the tail end of the light guide tube is also connected with a fruit fly guide, the fruit fly guider is in a round table shape and is made of a one-way light guide material, the diameter of a first round surface of the fruit fly guider is larger than that of a second round surface, the first round surface of the fruit fly guider in the light guide pipe is positioned in front of the second round surface, an opening of a cylindrical pipe where a yellow-green light source is located is over against the second round surface of the fruit fly guider connected with the tail end of the light guide pipe, and each yellow-green light source is connected with the control device and the power supply.
Optionally, the light pipe is provided with an electromagnetic valve, and the electromagnetic valve is connected with the control device and the power supply.
Optionally, the diameter of the second circular face of the drosophila guide is 3-4 mm.
Optionally, the lower part of the pipe plug is in threaded connection with the upper part of the fruit fly culture pipe.
Optionally, the electromagnetic valve is disposed at a connection of the plug and the light pipe.
Optionally, the plug is made of a gas permeable material.
The invention has the beneficial effects that:
through setting up structures such as fruit fly culture tube, stopcock, light pipe, yellow green light source, fruit fly director and controlling means, provide one kind and can carry out the system that sieves to the resistant strain of fruit fly, ensure through this system that the fruit fly strain of finally surviving is the resistant strain that passes through multistage screening survival.
Drawings
Fig. 1 is a schematic structural view of the present invention.
Fig. 2 is a schematic diagram of the circuit connection relationship according to the present invention.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples.
As shown in fig. 1 and fig. 2, the system for screening fruit fly resistant strains in the present embodiment includes a plurality of fruit fly culture tubes 1, a culture medium is placed in each fruit fly culture tube 1 arranged in sequence from left to right, a chemical is placed in the culture medium, the amount of the chemical placed in each fruit fly culture tube 1 arranged in sequence from left to right is gradually increased, the fruit fly culture tubes 1 are cylindrical, each fruit fly culture tube 1 is provided with a tube plug 2, the tube plug 2 is in an inverted funnel shape, the lower portion of the tube plug 2 is connected to the upper portion of the fruit fly culture tube 1, the upper portion of the previous tube plug 2 is connected to the front end of a light guide tube 3, the end of the light guide tube 3 is connected to the upper portion of the next fruit fly culture tube 1, the upper portions of the side walls of the other fruit fly culture tubes 1 except for the first fruit fly culture tube 1 are provided with yellow-green light sources 4, the yellow-green light sources 4 are placed in the cylindrical tube with an opening at one end, a plurality of fruit fly guides 5 are installed inside the light guide tube 3, the tail end of the light guide pipe 3 is also connected with a fruit fly guider 5, the fruit fly guider 5 is in a round table shape and is made of a one-way light guide material, the diameter of a first round surface of the fruit fly guider 5 is larger than that of a second round surface, the first round surface of the fruit fly guider 5 inside the light guide pipe 3 is positioned in front of the second round surface, an opening of a cylindrical pipe where the yellow-green light source 4 is located is over against the second round surface of the fruit fly guider 5 connected with the tail end of the light guide pipe 3, and each yellow-green light source 4 is connected with the control device 6 and the power supply 7. In fig. 1, the present invention is exemplified by including three drosophila culture tubes 1, and in the specific implementation, the number of the drosophila culture tubes 1 can be set according to the requirement.
Wherein the power supply 7 is used for supplying power to each yellow-green light source 4. The control device 6 is used for controlling the turn-on time and the turn-on duration of each yellow-green light source 4.
Optionally, the light pipe 3 is provided with an electromagnetic valve 8, and the electromagnetic valve 8 is connected with both the control device 6 and the power supply 7. The electromagnetic valve 8 is used for controlling whether to lead the fruit flies to the next fruit fly culture tube 1.
Optionally, the diameter of the second round surface of the fruit fly guide 5 is 3-4mm, so that 2-4 fruit flies can pass through the second round surface of the fruit fly guide 5 at the same time.
Optionally, the lower part of the pipe plug 2 is in threaded connection with the upper part of the fruit fly culture pipe 1.
Optionally, a solenoid valve 8 is provided at the junction of the plug 2 and the light pipe 3.
Wherein, the pipe plug 2 is made of a breathable material to ensure the normal breathing of the fruit flies.
When the fruit fly culture tube is used, a culture medium is firstly put into each fruit fly culture tube 1 which is sequentially arranged from left to right, a pesticide is put into the culture medium, the pesticide amount put into each fruit fly culture tube 1 which is sequentially arranged from left to right is gradually increased, and then the first generation fruit flies are put into the first fruit fly culture tube 1 for culture. After the first generation of fruit flies are cultured for a period of time and fruit flies with dosage capable of resisting the medicine in the first fruit fly culture tube 1 are left, the control device 6 controls the yellow-green light source 4 on the second fruit fly culture tube 1 to be turned on, and because the fruit flies have phototaxis (yellow-green light) and behavior preference tending to move upwards, the left fruit flies can move into the second fruit fly culture tube 1 through the light guide tube 3. Since the fruit flies have a preference to move from the wide surface to the narrow surface, by setting the diameter of the first circular surface of the fruit fly guide 5 to be larger than the diameter of the second circular surface, the fruit flies can be guided into the latter fruit fly culture tube 1 by the fruit fly guide 5 without generally moving in the opposite direction, so that the fruit flies generally tend to move in one direction. Since the fruit fly guide 5 is made of the unidirectional light guide material, the brightness of the second round surface of the fruit fly guide 5 is higher than that of the first round surface when viewed by fruit flies, and most of the fruit flies tend to enter the next fruit fly culture tube 1 through the second round surface of the fruit fly guide 5. And analogizing in turn until the drosophila melanogaster strain meeting certain drug resistance is screened out. When the electromagnetic valve 8 is arranged on the light guide pipe 3, if the fruit flies are required to be guided into the next fruit fly culture pipe 1, the control device 6 controls the electromagnetic valve 8 to be opened. The electromagnetic valve 8 is arranged at the joint of the pipe plug 2 and the light guide pipe 3, so that after the next fruit fly culture pipe 1 is led in the fruit flies and the electromagnetic valve 8 is closed, part of the fruit flies are prevented from being detained between the pipe plug 2 and the electromagnetic valve, and the part of the fruit flies are prevented from dying. The yellow-green light source 4 is arranged in the cylindrical tube with an opening at one end, the opening of the cylindrical tube with the yellow-green light source 4 is opposite to the second round surface of the fruit fly guider 5 connected with the tail end of the light guide tube 3, and light emitted by the yellow-green light source 4 can be directly irradiated into the second round surface of the fruit fly guider 5.
The method is based on phototaxis and movement behavior preference of the fruit fly imagoes, so that the fruit flies surviving after screening can be automatically transferred to the next fruit fly culture tube 1. Through multiple rounds of screening, the fruit fly survived in the culture medium with the highest concentration of the medicament is the resistant fruit fly which can tolerate the medicament, so that the invention can ensure that the finally remained fruit fly strain is the resistant strain survived through multiple rounds of screening. Of course, the invention can also be used for screening other insect strains with the same behavioral preference as fruit flies.
It will be understood that the above embodiments are merely exemplary embodiments taken to illustrate the principles of the present invention, which is not limited thereto. It will be apparent to those skilled in the art that various modifications and improvements can be made without departing from the spirit and substance of the invention, and these modifications and improvements are also considered to be within the scope of the invention.
Claims (6)
1. A drosophila resistant strain screening system is characterized by comprising a plurality of drosophila culture tubes (1), wherein a culture medium is placed in each drosophila culture tube (1) which is sequentially arranged from left to right, a pesticide is placed in the culture medium, the amount of the pesticide placed in each drosophila culture tube (1) which is sequentially arranged from left to right is gradually increased, the drosophila culture tubes (1) are cylindrical, each drosophila culture tube (1) is provided with a tube plug (2), the tube plug (2) is in an inverted funnel shape, the lower part of the tube plug (2) is connected with the upper part of the drosophila culture tube (1), the upper part of the last tube plug (2) is connected with the front end of the light guide tube (3), the tail end of the light guide tube (3) is connected with the upper part of the next drosophila culture tube (1), the upper parts of the side walls of other drosophila culture tubes (1) except the first drosophila culture tube (1) are provided with yellow-green light sources (4), and place in yellow green light source (4) in one end open-ended cylindrical tube, light pipe (3) internally mounted has a plurality of fruit bat director (5), the end of light pipe (3) also is connected with a fruit bat director (5), fruit bat director (5) are round platform shape and adopt one-way leaded light material to make, the diameter of the first disc of fruit bat director (5) is greater than the diameter of second disc, the first disc of the inside fruit bat director (5) of light pipe (3) is located before its second disc, the opening of the cylindrical tube that yellow green light source (4) were located is just to the second disc of the fruit bat director (5) that light pipe (3) end was connected, each yellow green light source (4) all are connected with controlling means (6) and power (7).
2. The drosophila-resistant strain screening system according to claim 1, wherein the light pipe (3) is provided with an electromagnetic valve (8), and the electromagnetic valve (8) is connected with both the control device (6) and the power supply (7).
3. The drosophila-resistant strain screening system according to claim 1, wherein the diameter of the second circular face of the drosophila guide (5) is 3-4 mm.
4. The drosophila-resistant strain screening system according to claim 1, wherein the lower part of the pipe plug (2) is screwed with the upper part of the drosophila culture pipe (1).
5. The drosophila-resistant strain screening system according to claim 2, wherein the solenoid valve (8) is arranged at the junction of the pipe plug (2) and the light pipe (3).
6. The drosophila-resistant strain screening system according to claim 1, wherein the plug (2) is made of a gas-permeable material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010097736.0A CN111149773B (en) | 2020-02-17 | 2020-02-17 | Drosophila resistance strain screening system |
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| CN202010097736.0A CN111149773B (en) | 2020-02-17 | 2020-02-17 | Drosophila resistance strain screening system |
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| CN111149773A CN111149773A (en) | 2020-05-15 |
| CN111149773B true CN111149773B (en) | 2021-11-19 |
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Effective date of registration: 20250113 Address after: No. 500, Linyang Road, Qidong Economic Development Zone, Nantong City, Jiangsu Province Patentee after: Qidong Fangjing Biotechnology Co.,Ltd. Country or region after: China Address before: 030006, No. 92, Hollywood Road, Xiaodian District, Shanxi, Taiyuan Patentee before: SHANXI University Country or region before: China |