CN111073815B - A cytological sub-magnetic effect experimental system - Google Patents
A cytological sub-magnetic effect experimental system Download PDFInfo
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- CN111073815B CN111073815B CN202010021516.XA CN202010021516A CN111073815B CN 111073815 B CN111073815 B CN 111073815B CN 202010021516 A CN202010021516 A CN 202010021516A CN 111073815 B CN111073815 B CN 111073815B
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Abstract
本发明涉及一种细胞学亚磁效应实验系统，包括亚磁实验组件及对照组件，亚磁实验组件包括端盖、实验组件样本架、圆柱管状线圈、隔磁部件、对流风扇及滑环；滑环及对流风扇固定于隔磁部件的一端；圆柱管状线圈安装于隔磁部件内部；实验组件样本架通过圆柱管状线圈内部的轨道安装于圆柱管状线圈内部；端盖扣接在隔磁部件的另一端；对照组件用于为实验的对照样本组建立实验环境，包括筒盖、对照组件样本架、轨道、传动块、圆筒；轨道粘接在圆筒内部，传动块固定在圆筒外表面的两端，对照组件样本架安装在轨道上，所述筒盖扣接在圆筒的开放端。本发明能够建立亚磁环境，并实现细胞培养环境的自动控制，支撑地面开展亚磁复合环境细胞学效应研究。
The present invention relates to a cytological sub-magnetic effect experimental system, including a sub-magnetic experimental component and a control component, the sub-magnetic experimental component includes an end cover, an experimental component sample rack, a cylindrical tubular coil, a magnetic isolation component, a convection fan and a slip ring; the slip ring and the convection fan are fixed to one end of the magnetic isolation component; the cylindrical tubular coil is installed inside the magnetic isolation component; the experimental component sample rack is installed inside the cylindrical tubular coil through a track inside the cylindrical tubular coil; the end cover is buckled on the other end of the magnetic isolation component; the control component is used to establish an experimental environment for the control sample group of the experiment, including a cylinder cover, a control component sample rack, a track, a transmission block, and a cylinder; the track is bonded inside the cylinder, the transmission block is fixed at both ends of the outer surface of the cylinder, the control component sample rack is installed on the track, and the cylinder cover is buckled on the open end of the cylinder. The present invention can establish a sub-magnetic environment, realize automatic control of the cell culture environment, and support the ground to carry out sub-magnetic composite environment cytological effect research.
Description
Technical Field
The invention belongs to the technical field of cytobiology, and particularly relates to a cytological sub-magnetic effect experimental system.
Background
With the continuous deep space exploration, the study of the cytological effect of the space environment, especially the cytological compound effect of the space environment, has been attracting more and more attention. However, the space real-time flight experiment has few opportunities and extremely limited experimental resource conditions, so that the simulation research of the related space compound effect is carried out on the ground, and the method becomes a necessary way for exploring the space mystery and accumulating the space knowledge in the related discipline.
The study of the cytological composite effect related to the space environment is carried out on the ground, and the necessary simulation equipment is needed to be used as a support, but no cytological effect study platform with perfect functions, in particular the capability of establishing different levels of low gravity and sub-magnetic composite environments, exists at present.
The present development of space microgravity effect simulation research often adopts a series of gyros (Rotate WALL VESSELS, RWVS) produced in the United states, while the development of the sub-magnetic cytology effect research adopts a shielding box and the like, and the technical defects of the development of space compound effect research by adopting the independent mutually independent devices include:
1) By adopting the experimental mode of the independent equipment, the experiment of the sub-magnetic environment effect cannot be effectively carried out, and the research of the cytological effect of the sub-magnetic is limited to be carried out on the ground.
2) The existing equipment does not integrate an automatic control system of a cell culture environment, can not autonomously maintain the conditions required by the cell culture experiment, and restricts the long-term development of related experiments.
Disclosure of Invention
The invention aims to provide a cytological sub-magnetic effect experimental system, which establishes a sub-magnetic environment, realizes automatic control of a cell culture environment and supports the ground to develop cytological effect research of the sub-magnetic environment.
The invention provides a cytological sub-magnetic effect experiment system which comprises a sub-magnetic experiment component and a comparison component, wherein the sub-magnetic experiment component is used for establishing a sub-magnetic environment with low magnetic field intensity meeting experiment requirements in a region where a cell sample is located, and comprises an end cover, an experiment component sample frame, a cylindrical tubular coil, a magnetism isolating component, a convection fan and a slip ring, wherein the slip ring and the convection fan are fixed at one end of the magnetism isolating component;
The control assembly is used for establishing an experimental environment for a control sample group of an experiment and comprises a cylinder cover, a control assembly sample frame, a track, a transmission block and a cylinder, wherein the track is adhered inside the cylinder, the transmission block is fixed at two ends of the outer surface of the cylinder, the control assembly sample frame is mounted on the track, and the cylinder cover is buckled at the open end of the cylinder.
Further, the end cover consists of an inner cover, a magnetism isolating plate and an outer cover, wherein the inner cover and the outer cover are respectively adhered to two sides of the magnetism isolating plate;
the experimental component sample rack is used for installing experimental samples and comprises a main body structure and a buffer piece, wherein the buffer piece is adhered to the main body structure;
the cylindrical tubular coil is used for generating a uniform magnetic field required by experiments and comprises a track, a support tube and an enameled wire spirally wound outside the support tube, and the track is adhered to the inner surface of the support tube;
The magnetism isolating component is used for isolating a magnetic field in an experimental environment and comprises a magnetism isolating layer, a protective outer layer, a protective inner layer and a transmission block, wherein the magnetism isolating layer is inlaid outside the protective inner layer, the protective outer layer is inlaid outside the magnetism isolating layer, and the transmission block is fixedly connected with two ends of the protective outer layer.
Further, the system also comprises a shell, wherein the shell is of a box structure, and the sub-magnetic experimental assembly and the control assembly are arranged in the shell and form a closed cell experimental area in the shell.
Further, the shell comprises a body structure, an upper cover, a rear cover, a hinge and a heat preservation layer, wherein the body structure is connected with the upper cover through the hinge, the body structure is connected with the rear cover through a screw, and the heat preservation layer is installed inside the body structure.
The system further comprises a cell culture environment control assembly and a control system, wherein the cell culture environment control assembly is used for establishing conditions of temperature and CO 2 concentration required by cell experiments in the cell experiment area, and the control system is used for regulating and controlling the cell culture environment control assembly to work according to the experiment requirements.
Further, the cell culture environment control assembly comprises a temperature control assembly and a CO 2 concentration control assembly, wherein the CO 2 concentration control assembly comprises a CO 2 gas cylinder, a pressure reducing valve, an electromagnetic valve, a CO 2 concentration sensor and a pressure pipe, the pressure reducing valve is arranged on the CO 2 gas cylinder, the pressure reducing valve is connected with the electromagnetic valve through the pressure pipe, the CO 2 concentration sensor is arranged in a cell experiment area in the shell, and the temperature control assembly is used for adjusting the temperature of the cell experiment area and comprises a heater, a convection fan and a temperature sensor, and the temperature sensor is arranged in the cell experiment area in the shell.
Further, the control system comprises a control circuit board and a display screen, wherein the control circuit board is arranged on the rear cover of the shell, the display screen is arranged on the upper cover of the shell, and the control circuit board is electrically connected with the cell culture environment control assembly and the display screen.
By means of the scheme, the cytomagnetic sub-magnetic effect experimental system can establish a sub-magnetic environment, realize automatic control of a cell culture environment and support the ground to develop cytomagnetic effect research of the sub-magnetic environment.
The foregoing description is only an overview of the present invention, and is intended to provide a better understanding of the present invention, as it is embodied in the following description, with reference to the preferred embodiments of the present invention and the accompanying drawings.
Drawings
FIG. 1 is a schematic diagram of the structure of a sub-magnetic experimental assembly of the present invention;
FIG. 2 is a schematic view of the sample rack of the present invention;
FIG. 3 is a schematic diagram of a control assembly according to the present invention;
FIG. 4 is a schematic view of the housing structure of the present invention;
FIG. 5 is a cytological effect experiment system of the present invention integrated with a low gravity experiment assembly;
FIG. 6 is a schematic diagram of the low gravity test assembly of the present invention;
FIG. 7 is a schematic view of the basic component structure of the present invention;
Fig. 8 is a schematic view of the structure of the transmission component of the present invention.
Reference numerals in the drawings:
1-a sub-magnetic experiment component, 11-an end cover, 12-an experiment component sample rack, 121-a main body structure, 122-a buffer piece, 13-a cylindrical tubular coil, 14-a magnetism isolating component, 15-a convection fan and 16-a slip ring;
2-comparison component, 21-cylinder cover, 22-comparison component sample rack, 23-track, 24-transmission block and 25-cylinder;
3-low gravity experiment components, 31-basic components, 311-bottom plates, 312-side plates, 313-driving support frames, 314-driven support frames, 315-bearings, 32-transmission components, 321-motors, 322-movable seats, 323-driving pulleys, 324-synchronous belts, 325-driven pulleys, 326-driving rotating ends, 327-driven rotating ends and 33-sample trays;
4-shell, 41-body structure, 42-upper cover, 43-back cover, 44-hinge and 45-heat insulation layer;
5-a control system;
6-cell culture environmental control module.
Detailed Description
The following describes in further detail the embodiments of the present invention with reference to the drawings and examples. The following examples are illustrative of the invention and are not intended to limit the scope of the invention.
Referring to fig. 1, the embodiment provides a cytological sub-magnetic effect experimental system, which comprises a sub-magnetic experimental assembly 1 and a control assembly 2, wherein the sub-magnetic experimental assembly is used for establishing a sub-magnetic environment with low magnetic field strength meeting experimental requirements in a region where a cell sample is located, and the control assembly is used for establishing an experimental environment for a control sample group of an experiment.
The sub-magnetic experimental assembly 1 consists of an end cover 11, an experimental assembly sample frame 12, a cylindrical tubular coil 13, a magnetism isolating part 14, a convection fan 15 and a slip ring 16. The slip ring 16 and the convection fan 15 are fixed at one end of the magnetism isolating part 14 through screws, the cylindrical tubular coil 13 is installed inside the magnetism isolating part 14, the experimental component sample rack 12 is installed inside the cylindrical tubular coil 13 through a track inside the cylindrical tubular coil 13 and used for installing experimental samples, and the end cover 11 is buckled at the other end (open end) of the magnetism isolating part 14, so that a closed space is formed inside the magnetism isolating part 14.
The magnetism isolating component 14 is used for isolating a magnetic field in an experimental environment and comprises a magnetism isolating layer, a protective outer layer, a protective inner layer and a transmission block, wherein the magnetism isolating layer is sleeved outside the protective inner layer in an inlaid manner, the protective outer layer is sleeved outside the magnetism isolating layer, and the transmission block is welded at two ends of the protective outer layer. The cylindrical tubular coil 13 is used for generating a uniform magnetic field required by experiments and comprises a track, a support tube and enameled wires spirally wound outside the support tube, and the track is adhered to the inner surface of the support tube. The end cover 11 is composed of an inner cover, a magnetism isolating plate and an outer cover, wherein the inner cover and the outer cover are respectively adhered to two sides of the magnetism isolating plate.
Referring to fig. 2, the experimental component sample rack 12 includes a main structure 121 and a buffer member 122, and the buffer member 122 is bonded to the main structure 121.
Referring to fig. 3, the control assembly 2 includes a cap 21, a control assembly sample holder 22, a rail 23, a drive block 24, and a cylinder 25. The track 23 is adhered inside the cylinder 25, the transmission blocks 24 are welded at two ends of the outer surface of the cylinder 25, the comparison component sample rack 22 is arranged on the track 23, and the cylinder cover 21 is buckled at the open end of the cylinder 25.
Referring to fig. 4, the housing 4 is a mounting base and protective structure of all the above components, the housing is a box structure, and the sub-magnetic experimental component 1 and the control component 2 can be arranged in the housing 4, and a closed cell experimental area is formed in the housing 4. Specifically, the housing 4 is composed of a body structure 41, an upper cover 42, a rear cover 43, a hinge 44 and a heat insulation layer 45, the body structure 41 is connected with the upper cover 42 through the hinge 44, the body structure 41 is connected with the rear cover 43 through screws, and the heat insulation layer 45 is installed inside the body structure 41.
Referring to fig. 5, a control system 5 and a cell culture environment control unit 6 may be provided to automatically control the cell culture environment.
Specifically, the cell culture environment control assembly 6 comprises a temperature control assembly and a CO 2 concentration control assembly, the CO 2 concentration control assembly comprises a CO 2 gas cylinder, a pressure reducing valve, an electromagnetic valve, a CO 2 concentration sensor and a pressure pipe, the pressure reducing valve is arranged on the CO 2 gas cylinder, the pressure reducing valve is connected with the electromagnetic valve through the pressure pipe, the CO 2 concentration sensor is arranged on the bottom plate 311 of the base part 31, CO 2 gas in the CO 2 gas cylinder flows into the electromagnetic valve through the pressure pipe after the pressure of the CO 2 gas is reduced through the pressure reducing valve, the control system 5 regulates the opening of the electromagnetic valve, after the electromagnetic valve is opened, the CO 2 gas flows into a cell experiment area enclosed by the base part 31 through a pipeline, the CO 2 concentration sensor detects, after the CO 2 concentration meets the experiment requirement, the control system 5 regulates the closing of the electromagnetic valve, the CO 2 gas is stopped, and the inflow into the cell experiment area enclosed by the base part 31 is stopped. The temperature control assembly consists of a heater, a convection fan and a temperature sensor and is used for adjusting the temperature of the cell experiment area.
The control system 5 is composed of a plurality of control circuit boards and a display screen, wherein the control circuit boards are arranged on the back (rear cover) of the shell 4, the display screen is arranged on the upper cover of the shell 4, and the control circuit boards are connected with the cell culture environment control assembly and the display screen through wires.
Reference 5 shows that in order to realize the simulation of the low gravity and the sub-magnetic composite environment and the automatic control of the cell culture environment, the low gravity experimental component 3 can be integrated on the basis of the cytological sub-magnetic effect experimental system. The system can independently carry out experiments without the auxiliary support of other peripheral equipment, realizes the automatic control of the cell culture environment, and supports the study of the cytological effect of the space low gravity and sub-magnetic composite environment on the ground.
Referring to fig. 6, the gravity test low assembly 3 includes a base member 31, 4 sets of transmission members 32 (which may be increased or decreased according to the test requirements), and a sample tray 33. The transmission member 32 is installed in the base member 31, and the sample tray 33 is installed at an upper portion of the transmission member 32. The base member 31 is a low gravity test assembly 3 mounting base for supporting the drive member 32 and sample tray 33 in the low gravity test assembly 3. The transmission part 32 is used for driving the sub-magnetic experiment assembly 1 and the comparison assembly 2 to rotate according to experiment requirements.
Referring to fig. 7, the base member 31 includes a bottom plate 311, a side plate 312, a driving support 313, a driven support 314, and a bearing 315, the base member 31 includes a rectangular box structure with an opening at an upper end, the bottom plate 311 is disposed on a bottom surface of the rectangular box structure, the driving support 313 and the driven support 314 are disposed on a set of opposite sides of the rectangular box, the 2 side plates 312 are disposed on another set of opposite sides of the rectangular box, the driving support 313 and the driven support 314 are provided with a plurality of bearing mounting holes, and the bearing 315 is mounted in the bearing mounting holes on the driving support 313 and the driven support 314.
Referring to fig. 8, the transmission member 32 includes a motor 321, a movable base 322, a driving pulley 323, a synchronous belt 324, a driven pulley 325, a driving rotation end 326 and a driven rotation end 327, wherein the output end of the motor 321 is fixed on the movable base 322 by a screw, the driving pulley 323 is mounted on an output shaft of the motor 321, the movable base 322 with the motor 321 and the driving pulley 323 is fixed on a driving support 313 by a screw, the driving rotation end 326 passes through a bearing inner hole of the driving support 313 by an end shaft thereof and is mounted with the driving support 313, the driving pulley 323 is mounted on an end shaft of the driving rotation end 326, the driven rotation end 327 passes through a bearing inner hole of the driven support 314 by an end shaft thereof and is mounted with the driven support 314, the synchronous belt 324 is mounted between the driving pulley 323 and the driven pulley 325, and sliding grooves matched with transmission blocks on the driving experiment assembly 1 and the comparison assembly 2 are respectively arranged on end faces of the driving rotation end 326 and the driven rotation end 327, so that the experiment assembly 1 and the comparison assembly 2 can be driven to rotate.
The heater and convection fan in the cell culture environment control module 6 are mounted on the driven support 314, and the temperature sensor is mounted at the middle position of the base member bottom plate 311. The control circuit board in the control system is electrically connected with the gravity experiment low component 3.
In a specific embodiment, the inner cover and the outer cover of the end cover 11 are both processed by ABS materials, the diameter of the outer cover is 120mm, the thickness of the outer cover is 5mm, the diameter of the inner cover is 85mm, the thickness of the inner cover is 5mm, the magnetism isolating plate is processed by 1J85 materials to be in a disc shape, the diameter of the magnetism isolating plate is 85mm, the thickness of the magnetism isolating plate is 1mm, and the magnetism isolating plate and the inner cover are sequentially and coaxially bonded together by 502 glue according to the sequence of the outer cover, the magnetism isolating plate and the inner cover.
The main body structure 121 of the experiment component sample rack 12 is formed by 3D printing of a resin material, 2T 25 cell culture flasks (the installation requirement of experiment samples of other specifications can be met by adjusting the size of the main body structure), the wire surface of a nylon thread gluing is used as a buffer layer (buffer piece 122), and after cutting, the nylon thread gluing is stuck to the inner surface of the main body structure 121 by using 502 glue, so that the T25 cell culture flasks cannot slip when the experiment component sample rack 12 moves. The control component sample holder 22 may be constructed in the same manner as the experimental component sample holder 12 and will not be described in detail herein.
The support tube of the cylindrical tubular coil 13 is made of organic glass material, the inner diameter is 85mm, the wall thickness is 2mm, the length is 400mm, the outer surface is spirally wound with 1 layer of enameled wire with the diameter of 3mm (enameled wires with other diameters can be wound in multiple layers), the groove-shaped track is formed by adopting an ABS material machine, the groove-shaped track is stuck on the inner surface of the support tube by using 502 glue, and the track and the conducting block are arranged on the same plane.
The magnetism isolating layer of the magnetism isolating part 14 is formed by bending A1 mm thick 1J85 plate into a round tube and welding by argon arc welding, the inner diameter of the magnetism isolating layer is 95mm, the length of the magnetism isolating layer is 400mm (a plurality of magnetism isolating layers can be bent according to the requirement), the inner diameter of the protection outer layer is 104mm, the outer diameter of the protection outer layer is 120mm, the length of the protection outer layer is 430mm, the inner diameter of the protection inner layer is 95mm, the outer diameter of the protection inner layer is 102mm, the length of the protection inner layer is 400mm, the transmission block is formed by machining the aluminum alloy 2A12, the length of the transmission block is 20mm, the thickness of the transmission block is 10mm, and the transmission block is welded at two ends of the outer surface of the protection outer layer by argon arc welding.
The convection fan 15 adopts 40406 series ultra-silent convection fans, the external dimensions are 40mm multiplied by 6mm, the working voltage is 12DCV, and the ultra-silent convection fans are fixed at one end of the protective outer layer of the magnetism isolating part 14 by 4M 3 screws.
The slip ring 16 adopts a 4-path cylindrical miniature slip ring with the diameter of 5mm and the length of 11.4mm,2 paths are used for supplying power to the cylindrical tubular coil 13, and 2 paths are used for supplying power to the convection fan 15. Slip ring 16 is glued at the center of one end of the protective outer layer of magnetic barrier 14 with 502 glue.
The cylinder 25 of the control assembly 2 is formed by machining with ABS material, the inner diameter is 85mm, the outer diameter is 95mm, the length is 400mm, the inner rail 23 is also formed by machining with ABS material, the length is 400mm, the inner rail is adhered to the inside of the cylinder 25 by 502 glue, the transmission block 24 is formed by machining with ABS material, the length is 20mm, the thickness is 10mm, the inner rail is adhered to two ends of the inner outer surface of the cylinder 25 by 502 glue, and the rail 23 and the transmission block 24 are on the same plane.
In the base member 31 of the low gravity test module 3, the driving support 313 and the driven support 314 are formed by machining an aluminum alloy material 2A12, the external dimension is 490mm×200mm×30mm, the internal hollow region is 480mm×200mm×20mm, 8 bearing holes are distributed on the upper part, and the aperture isThe driving support 313 and the driven support 314 are respectively provided with 8 rolling bearings 315, the outer diameter of each bearing 315 is 20mm, the inner diameter is 8mm, the thickness is 5mm, the bottom plate 311 is made of 2A12 materials, the length is 510mm, the width is 490mm, the thickness is 10mm, 2 side plates 312 are formed by machining 2A12, and the external dimensions are 510mm multiplied by 200mm multiplied by 3mm. The driving support 313 and the driven support 314 are fixed to both ends of the bottom plate 311 with 4M 4 screws, and the 2 side plates 312 are fixed to the sides of the driving support 313 and the driven support 314 with M3 screws.
The motor 321 of the transmission part 32 is a 24V direct current motor, the outer diameter is 38mm and the length is 60mm, the movable seat 322 is formed by machining of an aluminum alloy 2A12, the outer diameter is 50mm, the length is 30mm and the inner diameter is 38mm, the driving pulley 323 is formed by machining of an aluminum alloy 2A12, 24T-shaped teeth are arranged, the reference circle diameter is 28mm and the thickness is 10mm, the driven pulley 325 is formed by machining of an aluminum alloy 2A12, 72T-shaped teeth are arranged, the reference circle diameter is 84mm and the thickness is 10mm, the synchronous belt 324 is an XL series light carrier belt, the bandwidth is 8mm, the driving rotating end 326 and the driven rotating end 327 are formed by machining of an aluminum alloy 2A12, the outer diameter is 120mm and the thickness is 10mm, one end is provided with a chute matched with transmission blocks on the hypomagnetic experiment assembly 1 and the comparison assembly 2, the groove width is 10mm, and the other end is provided with a straight shaft with the diameter of 8mm and the length is 35mm. The motor 321 is fixed to the movable base 322 with 2M 3 set screws, and after the driving pulley 3223 is mounted on the motor shaft, the movable base 322 is mounted on the lower portion of one side of the driving support 313, and is fixed with 4M 3 screws. After the shaft of the driving rotation end 326 passes through the bearing hole at the upper portion of the driving support 313, the driven pulley 325 is mounted thereon, and is adhered to prevent slipping by 502. The timing belt 324 is installed between the driving pulley 323 and the driven pulley 325. The driven rotating end 327 is mounted to the driven support 314 by an end shaft passing through the bearing bore of the driven support 314 and is also bonded 502 to prevent slippage.
The sample tray 33 is formed by stamping a stainless steel plate with the thickness of 1mm, and small holes with the diameter of 10mm are uniformly distributed on the bottom of the tray.
The body structure 41, the upper cover 42 and the rear cover 43 of the shell 4 are all formed by adopting photosensitive resin through 3D printing, the wall thickness is 3mm, the upper cover 42 and the rear cover 43 are fixed on the body structure 41 through M3 screws, the heat preservation layer 45 is formed by adopting polyurethane foam materials through integral foaming, and the heat preservation layer 45 is arranged inside the body structure 41.
In the cell culture environment control assembly 6, a CO 2 gas cylinder is an aluminum flat bottom 2L pressure cylinder, a pressure reducing valve is made of bipolar stainless steel, an electromagnetic valve is a 12V normally closed type electromagnetic valve for gas, a CO 2 concentration sensor is an infrared detection type sensor, the measurement range is 0-10%, a pressure pipe adopts a polytetrafluoroethylene hard gas pipe with the outer diameter of 3mm, and the inner diameter is 1mm. The electromagnetic valve and the CO 2 concentration sensor are fixed on the bottom plate through 2M 2 screws.
The heater in the temperature control assembly adopts a polyimide film heater, the heating voltage is 24V, the heating resistance is 24 omega, the size is 40mm to 40mm, the heater is directly attached to the lower part of the driven support 314 through back glue, the 12V convection fan is arranged in front of the polyimide film heater and is fixed through 4M 3 screws, and the temperature sensor adopts a standard digital sensing module and is arranged in the middle of the bottom plate 311 through 4M 2 screws.
The working mode is as follows:
2 sub-magnetic experiment components 1 and 2 control components 2 are arranged on the transmission component 32, 3 sample frames are arranged in each sub-magnetic experiment component 1 and 2 control components, 6T 25 cell culture flasks are contained, 24T 25 samples are arranged on the 4 components in total, and a plurality of standby T25 cell samples can be placed on the sample tray 33 to serve as standby experiment samples. The upper cover 42 is closed, and a closed experiment area is formed in the equipment. The experimental parameters are input on the display screen according to the requirements, the control system 5 controls the 1 sub-magnetic experimental assembly 1 and the 1 comparison assembly 2 to rotate according to the set parameters, and the 1 sub-magnetic experimental assembly 1 and the 1 comparison assembly 2 keep static, so that experimental samples in four states of a normal experimental group, a low gravity experimental group, a sub-magnetic experimental group, a low gravity and sub-magnetic compound experimental group and standby samples cultured in the same environment can be synchronously established. Meanwhile, on the regulation and control of the control system 5, the temperature of a cell experiment area is controlled in the range of 37 ℃ plus or minus 0.5 ℃, the concentration of CO 2 is controlled in the range of 5% plusor minus 0.3%, and the environment condition meeting the growth of cells is formed in the cell experiment area, so that the long-term development of related experiments is ensured. In the experimental process, the experimental states are displayed on a display screen in real time.
The system has the following technical effects:
1) Different levels of low gravity experimental environments can be established;
2) The uniform sub-magnetic environment with different magnetic field intensities meeting the cytological experiment requirement can be established;
3) The cytological experimental environment of low gravity and sub-magnetic combination can be established;
4) The experimental samples in five states of a normal experimental group, a normal control group, a low gravity experimental group, a sub-magnetic experimental group, a low gravity and sub-magnetic composite experimental group can be established simultaneously;
5) The environment parameters of the cell experimental region can be regulated and controlled independently in the experimental process, the cell growth requirement is met, and the long-term development of related experiments is realized.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, and it should be noted that it is possible for those skilled in the art to make several improvements and modifications without departing from the technical principle of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.
Claims (1)
1. The cytological sub-magnetic effect experiment system is characterized by comprising a sub-magnetic experiment assembly and a comparison assembly, wherein the sub-magnetic experiment assembly is used for establishing a sub-magnetic environment with low magnetic field intensity meeting experiment requirements in a region where a cell sample is located, and comprises an end cover, an experiment assembly sample frame, a cylindrical tubular coil, a magnetism isolating part, a convection fan and a slip ring, wherein the slip ring and the convection fan are fixed at one end of the magnetism isolating part;
The control component is used for establishing an experimental environment for a control sample group of an experiment and comprises a cylinder cover, a control component sample frame, a track, a transmission block and a cylinder, wherein the track is adhered inside the cylinder, the transmission block is fixed at two ends of the outer surface of the cylinder, the control component sample frame is arranged on the track, and the cylinder cover is buckled at the open end of the cylinder;
The end cover consists of an inner cover, a magnetism isolating plate and an outer cover, wherein the inner cover and the outer cover are respectively adhered to two sides of the magnetism isolating plate;
the experimental component sample rack is used for installing experimental samples and comprises a main body structure and a buffer piece, wherein the buffer piece is adhered to the main body structure;
the cylindrical tubular coil is used for generating a uniform magnetic field required by experiments and comprises a track, a support tube and an enameled wire spirally wound outside the support tube, and the track is adhered to the inner surface of the support tube;
the magnetic isolation component is used for isolating a magnetic field in an experimental environment and comprises a magnetic isolation layer, a protective outer layer, a protective inner layer and a transmission block, wherein the magnetic isolation layer is inlaid and sleeved outside the protective inner layer, the protective outer layer is inlaid and sleeved outside the magnetic isolation layer, and the transmission block is fixedly connected with two ends of the protective outer layer;
The cytological sub-magnetic effect experimental system also comprises a shell, wherein the shell is of a box structure, and the sub-magnetic experimental component and the control component are arranged in the shell and form a closed cell experimental area in the shell;
the shell consists of a body structure, an upper cover, a rear cover, a hinge and a heat preservation layer, wherein the body structure is connected with the upper cover through the hinge, the body structure is connected with the rear cover through a screw, and the heat preservation layer is arranged in the body structure;
The cytological sub-magnetic effect experiment system further comprises a cell culture environment control assembly and a control system, wherein the cell culture environment control assembly is used for establishing temperature and CO 2 concentration conditions required by cell experiments in the cell experiment area, the control system is used for regulating and controlling the cell culture environment control assembly to work according to the experiment requirements, the cell culture environment control assembly comprises a temperature control assembly and a CO 2 concentration control assembly, the CO 2 concentration control assembly comprises a CO 2 gas cylinder, a pressure reducing valve, an electromagnetic valve, a CO 2 concentration sensor and a pressure pipe, the pressure reducing valve is arranged on the CO 2 gas cylinder, the pressure reducing valve is connected with the electromagnetic valve through the pressure pipe, the CO 2 concentration sensor is arranged in the cell experiment area in the shell, and the temperature control assembly is used for regulating the temperature of the cell experiment area and comprises a heater, a convection fan and a temperature sensor, and the temperature sensor is arranged in the cell experiment area in the shell;
The control system comprises a control circuit board and a display screen, wherein the control circuit board is arranged on the rear cover of the shell, the display screen is arranged on the upper cover of the shell, and the control circuit board is electrically connected with the cell culture environment control assembly and the display screen;
The cytological sub-magnetic effect experimental system is integrated with a low gravity experimental component and is used for simultaneously realizing the simulation of a low gravity and sub-magnetic composite environment and the automatic control of a cell culture environment; the low-gravity experimental assembly comprises a base part, a transmission part and a sample tray, wherein the transmission part is arranged in the base part, the sample tray is arranged on the upper part of the transmission part, the base part is used for supporting the transmission part and the sample tray in the low-gravity experimental assembly, the transmission part is used for driving the sub-magnetic experimental assembly and the contrast assembly to rotate according to experimental requirements, the base part comprises a bottom plate, side plates, a driving support frame, a driven support frame and a bearing, the base part comprises a rectangular box structure with an upper end opening, the bottom plate is arranged on the bottom surface of the rectangular box structure, the driving support frame and the driven support frame are arranged on one group of opposite sides of the rectangular box, two side plates are arranged on the other group of opposite sides of the rectangular box, the driving support frame and the driven support frame are provided with a plurality of bearing mounting holes, the bearing is arranged in the bearing mounting holes on the driving support frame and the driven support frame, the transmission part comprises a motor, a movable seat, a driving pulley, a synchronous belt pulley, a driven pulley, a driving rotating end and a driven rotating end, a motor output end, a motor is arranged on the motor output end and a driving pulley and a driven end, the motor is arranged on the motor through the movable support frame and the driving end and the driven end through a driving end through a screw through a bearing, the driving end and the driving end through the driving end and the bearing support through the fixed end and the driving end through the bearing support shaft, the synchronous belt is arranged between the driving belt wheel and the driven belt wheel, and sliding grooves matched with transmission blocks on the sub-magnetic experiment assembly and the comparison assembly are respectively arranged on the end surfaces of the driving rotating end and the driven rotating end and are used for driving the sub-magnetic experiment assembly and the comparison assembly to rotate;
the heater and the convection fan in the cell culture environment control assembly are arranged on the driven support frame, the temperature sensor is arranged in the middle of the bottom plate of the foundation part, and the control circuit board in the control system is electrically connected with the gravity experiment low assembly.
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