CN101993823B - Environmental self-control type cytology experimental platform - Google Patents

Abstract

A cytology experiment platform with the ability to work independently and automatically regulate the cell culture environment. It is characterized by including cell culture environment self-regulation system, quasi-open cell culture system, multi-parameter monitoring system and cell culture area; cell culture environment self-regulation system includes gas composition control system and temperature control system; quasi-open cell culture The system uses semi-permeable membrane technology as the supporting cell culture system, including special cell culture bottles, sample holders, semi-permeable membranes and sealing caps. The sample holder is built into the cell culture bottle, and the semi-permeable membrane is fixed inside the sealing cap; The multi-parameter monitoring system is composed of microprocessor, A/D conversion, D/A conversion, parameter controller and parameter sensor. The remarkable feature of the invention is that it has independent working ability, can be used for simulation research of microgravity effect in ground cytology, and can automatically regulate the cell culture environment to ensure the normal growth of cells.

Description

Environment self-controlled cytology experiment platform

Technical Field The present invention relates to a cell experiment platform used in the research fields of life science and cell biology, especially a cell microgravity effect simulation platform with the ability to autonomously regulate the cell culture environment. the

Background technology Limited by factors such as few real-time experiment opportunities, large investment, and high risk in aerospace medical cytology space, ground-based simulation research has become a prerequisite and an important supplement for space-based research. more urgent needs. At present, the development of ground-based simulation platform has become a research hotspot at home and abroad, especially the ground-based simulation research platform for aerospace medical cytology research at the cell molecular level has become the focus of attention in this field. the

The development of foreign microgravity effect ground-based simulation platforms is represented by the series of Rotate Wall Vessels (RWVs) developed by NASA. Different series and models of ground-based simulation equipment have been developed in this field to serve different medical research goals. It is precisely because of the widespread use of the above-mentioned equipment that its limitations have gradually emerged. First of all, the existing equipment cannot autonomously regulate the cell culture environment, and cannot meet the needs of medium and long-term aerospace medical cytology research. The existing equipment adopts a fully sealed cell culture mode, and the stability of the cell growth environment is achieved through manual replacement of the cell culture medium, which will affect the smooth implementation of subsequent cytology experiments for a longer period of time. the

Second, the existing equipment cannot work independently and needs to be used in conjunction with auxiliary equipment, and cannot be integrated into submagnetic equipment and artificial radiation equipment, which cannot meet the needs of the urgent research on the spatial compound effect of cytology in aerospace medicine. the

SUMMARY OF THE INVENTION The purpose of the present invention is to establish an environment self-controlled cytology experiment platform, which can be used for the simulation research of microgravity effects in ground cytology, and has the ability of independent work and automatic regulation of cell culture environment, and supports the ground research of aerospace medical cytology. The platform mainly includes cell culture environment self-regulation system, quasi-open cell culture system, multi-parameter monitoring system and cell culture area. the

The self-regulating system of the cell culture environment in the equipment is the executive mechanism to maintain the necessary growth environment of the cells, mainly including the gas composition control system and the humidity control system. The goal of the gas composition control system is to regulate the _CO2 concentration. It is composed of a gas cylinder, a pressure pipe, a pressure reducing valve, a solenoid valve, a filter, and a _CO2 sensor. All components are connected in sequence through the pressure pipe. The _CO2 gas flowing out of the gas cylinder flows through the pressure pipe through the pressure reducing valve and then reaches the solenoid valve. When the equipment requires gas supply, the solenoid valve opens, and the _CO2 gas is filtered by the filter and injected into the cell culture area of the equipment. After the concentration meets the requirements, the solenoid valve closes and the equipment automatically stops the gas supply. The temperature control system is composed of a heat preservation structure, a heater, a radiator, a fan and a sensor. The heat preservation structure is wrapped outside the cell culture area, the heater, radiator and fan are fixed at a corner of the cell culture area, and the sensor is installed in the cell culture area. inside.

The quasi-open cell culture system is supported by semi-permeable membrane technology, including special cell culture flasks, sample holders, semi-permeable membranes and sealing caps. The sample holder is built into the cell culture bottle, and the semi-permeable membrane is fixed inside the sealing cap. the

The function of the multi-parameter monitoring system is to monitor the working state parameters of the equipment, mainly including _CO2 concentration, cell culture temperature and motor working speed, etc. The multi-parameter monitoring system is composed of microprocessor, A/D conversion, D/A conversion, parameter controller and parameter sensor. The system collects data on temperature, CO ₂ concentration and rotational speed, and compares it with the set value; adjusts and controls the action of the actuator so that the actual value tends to the set value.

After the present invention adopts above-mentioned technical scheme, its notable feature is:

1) It has the ability to work independently, and does not depend on other equipment for normal work;

2) It can automatically regulate the cell culture environment to ensure the normal growth of cells. the

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic diagram of the overall structure of the present invention. the

Embodiment The gas cylinder 2 adopted by the gas component control system in the present invention is a 300ml high-pressure gas cylinder, which can withstand 300 atmospheres. The pressure reducing valve 1 is connected with the gas cylinder 2 through an M18 thread, and the outlet of the pressure reducing valve 1 is connected to the solenoid valve 4 The air inlets are connected with copper pressure tubes 3 with an outer diameter of 5 mm. The gas flowing out of the solenoid valve 4 is filtered by a 22 μm filter 5 and injected into the cell culture area 6. The CO ₂ sensor 16 uses a non-diffusion infrared sensor.

The outer insulation material 11 of the cell culture area 6 is directly foamed with polyurethane foam, the heater 12 is a 220V film electric heater with a heating area of 5×5mm ² , the radiator 13 is an aluminum straight-fin radiator, and the heater 12 The space between the radiator 13 and the radiator 13 is coated with thermal conductive silicone grease and fixed with 4 M2 screws. The fan 14 is a 12V axial flow fan. The heater 12 and radiator 13 components are fixed on the outlet of the fan 14 with 2 M3 screws. The temperature sensor 15 is fixed on the inner side of the cell culture area 6 with heat-conducting glue.

In the quasi-open cell culture system, the cell culture bottle 7 is injection-molded with medical polycarbonate, with an inner diameter of 30 mm, a height of 40 mm, and a wall thickness of 2 mm. The sample holder 8 is also injection-molded by medical polycarbonate, and can hold 6 samples. The semi-permeable membrane 9 is processed by medical silicone rubber and has a thickness of 0.1 mm. The sealing cap 10 is connected with the cell culture bottle 7 through an M36 thread. After the sealing cap 10 is tightened, the semi-permeable membrane 9 is pressed against the cell culture bottle 7 bottlenecks. the

When the equipment is working, the heater 12 starts to heat, and the heat is conducted to the radiator 13, and then the fan 14 forms forced convection in the cell culture area 6, so that the temperature of the cell culture _area is maintained at 36.5°C±0.5°C; 2, after being decompressed by the pressure reducing valve 1, it reaches the solenoid valve 4. When the cell culture area 6 requires supplementing CO ₂ , the multi-parameter monitoring system controls the solenoid valve 4 to open, and the CO ₂ gas flows through the solenoid valve 4 and passes through the filter 5 After filtering, inject it into the cell culture area 6, and ensure that the _CO2 concentration in the cell culture area is maintained at a level of 5% ± 0.3%. CO ₂ enters the cell culture flask 7 through the semipermeable membrane 9 to meet the requirements of cell growth.

After the implementation of the above scheme, the equipment has the ability to work independently, and can automatically adjust the environment of the cell culture area to meet the normal growth requirements of cells. the

Claims (1)

1. An environmental self-control type cytology experiment platform, comprising a cell culture environment self-regulation system, a quasi-open cell culture system, a multi-parameter monitoring system and a cell culture area, characterized in that: the cell culture environment self-regulation system includes Gas composition control system and temperature control system; the gas composition control system consists of gas cylinders, pressure pipes, pressure reducing valves, solenoid valves, filters, and CO2 sensors, and all components are connected in sequence through pressure pipes; the temperature control system consists of heat preservation structures, Composed of heater, radiator, fan and sensor, the insulation structure is wrapped outside the cell culture area, the heater, radiator and fan are fixed at a corner of the cell culture area, and the sensor is installed inside the cell culture area; the quasi-open cell culture The system uses semi-permeable membrane technology as a supporting cell culture system, including special cell culture bottles, sample holders, semi-permeable membranes and sealing covers. The sample holders are built into the cell culture bottles, and the semi-permeable membranes are fixed inside the sealing covers; The function of the multi-parameter monitoring system is to monitor the working state parameters of the equipment, which is composed of a microprocessor, A/D conversion, D/A conversion, parameter controller and parameter sensor.

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