CN110856463A - Tissue culture and rapid propagation method of red raspberries - Google Patents
Tissue culture and rapid propagation method of red raspberries Download PDFInfo
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- 244000235659 Rubus idaeus Species 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 18
- 235000015810 grayleaf red raspberry Nutrition 0.000 title claims abstract description 10
- 241000196324 Embryophyta Species 0.000 claims abstract description 132
- 230000001954 sterilising effect Effects 0.000 claims abstract description 35
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 30
- 230000001678 irradiating effect Effects 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000004140 cleaning Methods 0.000 claims abstract description 6
- 238000013138 pruning Methods 0.000 claims abstract description 6
- 238000009966 trimming Methods 0.000 claims abstract description 6
- 239000002689 soil Substances 0.000 claims description 55
- 238000012258 culturing Methods 0.000 claims description 21
- 238000005286 illumination Methods 0.000 claims description 20
- RDXARWSSOJYNLI-UHFFFAOYSA-N [P].[K] Chemical compound [P].[K] RDXARWSSOJYNLI-UHFFFAOYSA-N 0.000 claims description 14
- 239000004927 clay Substances 0.000 claims description 14
- 239000003337 fertilizer Substances 0.000 claims description 14
- 239000000618 nitrogen fertilizer Substances 0.000 claims description 14
- 235000011034 Rubus glaucus Nutrition 0.000 claims description 9
- 235000009122 Rubus idaeus Nutrition 0.000 claims description 9
- 230000004083 survival effect Effects 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 5
- 239000003640 drug residue Substances 0.000 abstract 1
- 239000003814 drug Substances 0.000 description 8
- 244000291333 Serissa japonica Species 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 230000008569 process Effects 0.000 description 3
- 230000001902 propagating effect Effects 0.000 description 3
- 239000003864 humus Substances 0.000 description 2
- 235000021013 raspberries Nutrition 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/08—Devices for filling-up flower-pots or pots for seedlings; Devices for setting plants or seeds in pots
- A01G9/086—Devices for repotting
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Developmental Biology & Embryology (AREA)
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- Forests & Forestry (AREA)
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Abstract
The invention discloses a tissue culture and rapid propagation method of red raspberries, which specifically comprises the following steps: step one, mother plant collection: collecting a root seedling stock plant of an underground stem section of a red raspberry plant; step two, pruning the stock plant: properly trimming the root system and the overground position of the stock plant of the bunch root seedling collected in the step one; step three, mother plant sterilization: and (4) cleaning the root-string seedling stock plant trimmed in the second step by using clear water, and then irradiating the tip or the root-string seedling for 5-7 minutes by using ultraviolet sterilization equipment. The invention ensures the sterilization effect in actual operation, has no drug residue, does not influence the subsequent growth of the stock plant of the bunch root seedling, does not reduce the survival rate of the stock plant of the bunch root seedling, and ensures the survival rates of the bunch root stock plant, the single bunch root seedling and the plant capable of being planted.
Description
Technical Field
The invention relates to the technical field of tissue culture and rapid propagation of plants, in particular to a tissue culture and rapid propagation method of red raspberries.
Background
The red raspberry is also called raspberry, is shrub and is 1-2 meters high; the branches are brown or reddish brown, and are fluffy, short, soft and smooth when young, and are thinned with skin thorns. 3-7 small leaves, 3 small leaves are sometimes arranged on flowering branches, 5-7 small leaves are often arranged on sterile branches, the shape of the long oval or the ellipse, the shape of the terminal small leaves is often oval, sometimes the shape of the short and sharp top ends is 3-8 cm long, the width is 1.5-4.5 cm, the base part is circular, the base part of the terminal small leaves is heart-shaped, the upper surface has no hair or loose and soft hair, the lower surface is densely covered with grey white hair, and irregular coarse saw teeth or heavy saw teeth are arranged on the edge; the petiole is 3-6 cm long, and the apical leaflet petiole is about 1 cm long and is all villiform short, soft and sparse; the leaf supporting line is shaped like a line and has short and soft hair.
In the prior art, the tissue culture and rapid propagation method for raspberries still has some defects in actual execution, for example, the stock plant of the bunch root seedling is usually sterilized when being transplanted, most of the existing sterilization modes for the stock plant of the bunch root seedling are medicament sterilization, and the medicament residue after sterilization can affect the subsequent growth of the stock plant of the bunch root seedling, so that the survival rate of the stock plant of the bunch root seedling is reduced.
Therefore, it is necessary to invent a tissue culture and rapid propagation method of raspberry to solve the above problems.
Disclosure of Invention
The invention aims to provide a tissue culture and rapid propagation method of raspberries, which replaces the traditional medicine sterilization mode by an ultraviolet sterilization mode, ensures the sterilization effect in actual operation, does not have the condition of medicine residue, does not influence the subsequent growth of the stock plant of the bunch root seedling, does not cause the reduction of the survival rate of the stock plant of the bunch root seedling, and ensures the survival rates of the stock plant of the bunch root seedling, the single bunch root seedling and the plantable plant, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a tissue culture and rapid propagation method of red raspberries specifically comprises the following steps:
step one, mother plant collection: collecting a root seedling stock plant of an underground stem section of a red raspberry plant;
step two, pruning the stock plant: properly trimming the root system and the overground position of the stock plant of the bunch root seedling collected in the step one;
step three, mother plant sterilization: cleaning the stock plant of the root-string seedling trimmed in the second step by using clear water, and then irradiating the tip or the root-string seedling for 5-7 minutes by using ultraviolet sterilization equipment;
step four, transplanting the stock plant: transplanting the sterilized bunch root seedling stock plant in the third step into culture soil in a culture tank, wherein the components of the culture soil comprise 55-58% of clay soil, 0.3-0.5% of nitrogen fertilizer, 0.1-0.15% of phosphorus-potassium fertilizer and the balance of sandy soil;
step five, mother plant culture: keeping the temperature in the culture tank at 22-28 ℃, the illumination intensity at 1600-1800 lux and the illumination time at 8-10 hours, and culturing the root-crossing seedling mother plant for 10-15 days;
step six, dividing parent plants: dividing the stock plant of the string root seedling cultured in the step five into a plurality of plants to obtain a plurality of single-plant string root seedlings;
seventhly, transplanting the single-plant root-bunch seedlings: transplanting the multiple single-plant string-root seedlings obtained in the sixth step into culture soil again, wherein the components of the culture soil comprise 60-65% of clay soil, 0.5-0.7% of nitrogen fertilizer, 0.2-0.24% of phosphorus-potassium fertilizer and the balance of sandy soil;
step eight, culturing single-plant root-string seedlings: keeping the temperature in the culture tank at 22-28 ℃, the illumination intensity at 1600-1800 lux and the illumination time at 8-10 hours, and culturing the stock plant of the root-string seedling for 20-22 days to obtain a plurality of rooting root-string seedlings;
step nine, outdoor planting: transferring the rooted serial root seedlings obtained in the step eight to the outdoor through a culture tank for further planting;
step ten, sterilizing the single-plant root-string seedlings: lifting the outdoor planted root-stringing seedlings out of the culture soil, refreshing the root-stringing seedlings by using clear water, and irradiating tip tips or the root-stringing seedlings for 4-6 minutes by using ultraviolet sterilization equipment to obtain a plurality of plants capable of being planted;
eleven, transplanting plants which can be planted: and D, replanting the multiple plants which can be planted and are obtained in the step ten into seedling bags to obtain the raspberry seedlings.
Preferably, the collection time in the first step is set from the end of autumn to the early spring.
Preferably, the planting time in the ninth step lasts 7-14 days.
The invention has the technical effects and advantages that:
1. the method has the advantages that the ultraviolet sterilization mode is used for replacing the traditional medicine sterilization mode in the prior art, the sterilization effect is ensured during actual operation, meanwhile, the condition of medicine residue does not exist, the subsequent growth of the cluster root seedling stock plant is not influenced, the survival rate of the cluster root seedling stock plant is not reduced, and the survival rates of the cluster root stock plant, the single-plant cluster root seedling and the plant capable of being planted are ensured;
2. the culture soil comprising the clay soil, the nitrogen fertilizer, the phosphorus-potassium fertilizer and the sandy soil is arranged, so that the nutrient components required by plants can be provided due to more humus in the clay soil, the nitrogen fertilizer and the phosphorus-potassium fertilizer can supplement required nutrient elements for the plants, and the sandy soil is convenient for providing a good growth environment for the stock plants and the single-plant string-root seedlings after the stock plants are transplanted and the single-plant string-root seedlings are transplanted.
Drawings
FIG. 1 is a schematic flow chart of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The invention provides a tissue culture and rapid propagation method of red raspberries as shown in figure 1, which comprises the following steps:
step one, mother plant collection: collecting the root seedling stock plant of the underground stem section of the red raspberry plant, wherein the collection time is set from late autumn to early spring;
step two, pruning the stock plant: properly trimming the root system and the overground position of the stock plant of the bunch root seedling collected in the step one;
step three, mother plant sterilization: cleaning the stock plant of the root-string seedling trimmed in the second step by using clear water, and then irradiating the tip or the root-string seedling for 5 minutes by using ultraviolet sterilization equipment;
step four, transplanting the stock plant: transplanting the sterilized bunch root seedling stock plant in the third step into culture soil in a culture tank, wherein the components of the culture soil comprise 55% of clay soil, 0.3% of nitrogen fertilizer, 0.1% of phosphorus-potassium fertilizer and the balance of sandy soil;
step five, mother plant culture: keeping the temperature in the culture tank at 22 ℃, the illumination intensity at 1600 lux and the sunlight illumination time at 8 hours, and culturing the stock plant of the bunch root seedling for 10 days;
step six, dividing parent plants: dividing the stock plant of the string root seedling cultured in the step five into a plurality of plants to obtain a plurality of single-plant string root seedlings;
seventhly, transplanting the single-plant root-bunch seedlings: transplanting the multiple single-plant string-root seedlings obtained in the sixth step into culture soil again, wherein the components of the culture soil comprise 60-6% of clay soil, 0.5% of nitrogen fertilizer, 0.2% of phosphorus-potassium fertilizer and the balance of sandy soil;
step eight, culturing single-plant root-string seedlings: maintaining the temperature in the culture tank at 22 ℃, the illumination intensity at 1600 lux and the sunlight illumination time at 8 hours, and culturing the stock plant of the bunch root seedling for 20 days to obtain a plurality of rooting bunch root seedlings;
step nine, outdoor planting: transferring the rooted serial root seedlings obtained in the step eight to the outdoor through a culture tank for further planting for 7 days;
step ten, sterilizing the single-plant root-string seedlings: lifting the outdoor planted root-stringing seedlings out of the culture soil, refreshing the root-stringing seedlings by using clear water, and irradiating tip tips or the root-stringing seedlings for 4 minutes by using ultraviolet sterilization equipment to obtain a plurality of plants capable of being planted;
eleven, transplanting plants which can be planted: and D, replanting the multiple plants which can be planted and are obtained in the step ten into seedling bags to obtain the raspberry seedlings.
Selecting 50 serials of the stock plants of the string root seedlings, then culturing and propagating according to the technical scheme, wherein the survival number of the stock plants of the string root seedlings in the culturing process is 45, obtaining 225 single-plant serial root seedlings after dividing the 45 stock plants of the string root seedlings, and finally growing 220 plants which can be planted.
Example 2
The invention provides a tissue culture and rapid propagation method of red raspberries as shown in figure 1, which comprises the following steps:
step one, mother plant collection: collecting the root seedling stock plant of the underground stem section of the red raspberry plant, wherein the collection time is set from late autumn to early spring;
step two, pruning the stock plant: properly trimming the root system and the overground position of the stock plant of the bunch root seedling collected in the step one;
step three, mother plant sterilization: cleaning the stock plant of the root-string seedling trimmed in the second step by using clear water, and then irradiating the tip or the root-string seedling for 6 minutes by using ultraviolet sterilization equipment;
step four, transplanting the stock plant: transplanting the sterilized bunch root seedling stock plant in the third step into culture soil in a culture tank, wherein the components of the culture soil comprise 56% of clay soil, 0.4% of nitrogen fertilizer, 0.13% of phosphorus potassium fertilizer and the balance of sandy soil;
step five, mother plant culture: keeping the temperature in the culture tank at 25 ℃, the illumination intensity at 1700 lux and the illumination time at 9 hours, and culturing the stock plant of the serial root seedling for 13 days;
step six, dividing parent plants: dividing the stock plant of the string root seedling cultured in the step five into a plurality of plants to obtain a plurality of single-plant string root seedlings;
seventhly, transplanting the single-plant root-bunch seedlings: transplanting the multiple single-plant string-root seedlings obtained in the sixth step into culture soil again, wherein the components of the culture soil comprise 63% of clay soil, 0.6% of nitrogen fertilizer, 0.22% of phosphorus-potassium fertilizer and the balance of sandy soil;
step eight, culturing single-plant root-string seedlings: maintaining the temperature in the culture tank at 25 ℃, the illumination intensity at 1700 lux and the sunlight illumination time at 9 hours, and culturing the stock plant of the bunch root seedling for 21 days to obtain a plurality of rooting bunch root seedlings;
step nine, outdoor planting: transferring the rooted serial root seedlings obtained in the step eight to the outdoor through a culture tank for further planting for 11 days;
step ten, sterilizing the single-plant root-string seedlings: lifting the outdoor planted root-stringing seedlings out of the culture soil, refreshing the root-stringing seedlings by using clear water, and irradiating tip tips or the root-stringing seedlings for 5 minutes by using ultraviolet sterilization equipment to obtain a plurality of plants capable of being planted;
eleven, transplanting plants which can be planted: and D, replanting the multiple plants which can be planted and are obtained in the step ten into seedling bags to obtain the raspberry seedlings.
Selecting 50 serissa serissoide mother plants, then culturing and propagating according to the technical scheme, wherein the survival number of the serissa serissoide mother plants is 49 in the culturing process, 265 single serissa serissoide is obtained after the 49 serissa serissoide mother plants are divided, and finally 263 plants which can be planted are grown.
Example 3
The invention provides a tissue culture and rapid propagation method of red raspberries as shown in figure 1, which comprises the following steps:
step one, mother plant collection: collecting the root seedling stock plant of the underground stem section of the red raspberry plant, wherein the collection time is set from late autumn to early spring;
step two, pruning the stock plant: properly trimming the root system and the overground position of the stock plant of the bunch root seedling collected in the step one;
step three, mother plant sterilization: cleaning the stock plant of the root-string seedling trimmed in the second step by using clear water, and then irradiating the tip or the root-string seedling for 7 minutes by using ultraviolet sterilization equipment;
step four, transplanting the stock plant: transplanting the sterilized bunch root seedling stock plant in the third step into culture soil in a culture tank, wherein the components of the culture soil comprise 58% of clay soil, 0.5% of nitrogen fertilizer, 0.15% of phosphorus potassium fertilizer and the balance of sandy soil;
step five, mother plant culture: keeping the temperature in the culture tank at 28 ℃, the illumination intensity at 1800 lux and the illumination time at 10 hours, and culturing the stock plant of the bunch root seedling for 15 days;
step six, dividing parent plants: dividing the stock plant of the string root seedling cultured in the step five into a plurality of plants to obtain a plurality of single-plant string root seedlings;
seventhly, transplanting the single-plant root-bunch seedlings: transplanting the multiple single-plant string-root seedlings obtained in the sixth step into culture soil again, wherein the components of the culture soil comprise 65% of clay soil, 0.7% of nitrogen fertilizer, 0.24% of phosphorus-potassium fertilizer and the balance of sandy soil;
step eight, culturing single-plant root-string seedlings: maintaining the temperature in the culture tank at 28 ℃, the illumination intensity at 1800 lux and the sunlight illumination time at 10 hours, and culturing the stock plant of the bunch root seedling for 22 days to obtain a plurality of rooting bunch root seedlings;
step nine, outdoor planting: transferring the rooted serial root seedlings obtained in the step eight to the outdoor through a culture tank for further planting for 14 days;
step ten, sterilizing the single-plant root-string seedlings: lifting the outdoor planted root-stringing seedlings out of the culture soil, refreshing the root-stringing seedlings by using clear water, and irradiating tip tips or the root-stringing seedlings for 4-6 minutes by using ultraviolet sterilization equipment to obtain a plurality of plants capable of being planted;
eleven, transplanting plants which can be planted: and D, replanting the multiple plants which can be planted and are obtained in the step ten into seedling bags to obtain the raspberry seedlings.
Selecting 50 serials of the stock seedlings, culturing and propagating according to the technical scheme, wherein the survival number of the serials of the stock seedlings is 46 in the culturing process, obtaining 240 single serials of the stock seedlings after the 46 serials of the stock seedlings are separated, and finally growing into 236 plants capable of being planted.
The following table can be derived from the above examples 1 to 3:
as can be seen from the above table, in examples 1 to 3, the root-bunch seedling stock plant processed by each item of data in example 2 has the largest survival quantity, the largest number of single root-bunch seedlings after plant division and the largest number of plants capable of being planted, the ultraviolet sterilization mode is used to replace the traditional medicine sterilization mode, so that the sterilization effect in actual operation is ensured, no medicine residue is left, the subsequent growth of the root-bunch seedling stock plant is not affected, the survival rate of the root-bunch seedling stock plant is not reduced, the survival rates of the root-bunch seedling stock plant, the single root-bunch seedling and the plant capable of being planted are ensured, meanwhile, the culture soil comprising clay soil, nitrogen fertilizer, phosphorus-potassium fertilizer and sand soil is provided, as the clay soil has more humus, the required nutrient components can be provided for the plants, and the nitrogen fertilizer and the phosphorus-potassium fertilizer can supplement the required nutrient elements for the plants, and the sandy soil is used for providing a good growth environment for the stock plant and the single-plant root-string seedling after the stock plant is transplanted and the single-plant root-string seedling is transplanted.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (3)
1. A tissue culture and rapid propagation method of red raspberries is characterized by comprising the following steps:
step one, mother plant collection: collecting a root seedling stock plant of an underground stem section of a red raspberry plant;
step two, pruning the stock plant: properly trimming the root system and the overground position of the stock plant of the bunch root seedling collected in the step one;
step three, mother plant sterilization: cleaning the stock plant of the root-string seedling trimmed in the second step by using clear water, and then irradiating the tip or the root-string seedling for 5-7 minutes by using ultraviolet sterilization equipment;
step four, transplanting the stock plant: transplanting the sterilized bunch root seedling stock plant in the third step into culture soil in a culture tank, wherein the components of the culture soil comprise 55-58% of clay soil, 0.3-0.5% of nitrogen fertilizer, 0.1-0.15% of phosphorus-potassium fertilizer and the balance of sandy soil;
step five, mother plant culture: keeping the temperature in the culture tank at 22-28 ℃, the illumination intensity at 1600-1800 lux and the illumination time at 8-10 hours, and culturing the root-crossing seedling mother plant for 10-15 days;
step six, dividing parent plants: dividing the stock plant of the string root seedling cultured in the step five into a plurality of plants to obtain a plurality of single-plant string root seedlings;
seventhly, transplanting the single-plant root-bunch seedlings: transplanting the multiple single-plant string-root seedlings obtained in the sixth step into culture soil again, wherein the components of the culture soil comprise 60-65% of clay soil, 0.5-0.7% of nitrogen fertilizer, 0.2-0.24% of phosphorus-potassium fertilizer and the balance of sandy soil;
step eight, culturing single-plant root-string seedlings: keeping the temperature in the culture tank at 22-28 ℃, the illumination intensity at 1600-1800 lux and the illumination time at 8-10 hours, and culturing the stock plant of the root-string seedling for 20-22 days to obtain a plurality of rooting root-string seedlings;
step nine, outdoor planting: transferring the rooted serial root seedlings obtained in the step eight to the outdoor through a culture tank for further planting;
step ten, sterilizing the single-plant root-string seedlings: lifting the outdoor planted root-stringing seedlings out of the culture soil, refreshing the root-stringing seedlings by using clear water, and irradiating tip tips or the root-stringing seedlings for 4-6 minutes by using ultraviolet sterilization equipment to obtain a plurality of plants capable of being planted;
eleven, transplanting plants which can be planted: and D, replanting the multiple plants which can be planted and are obtained in the step ten into seedling bags to obtain the raspberry seedlings.
2. The tissue culture and rapid propagation method of raspberry according to claim 1, characterized in that: and in the first step, the collection time is set from the end of autumn to the early spring.
3. The tissue culture and rapid propagation method of raspberry according to claim 1, characterized in that: the planting time in the ninth step lasts for 7-14 days.
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| CN101695280A (en) * | 2009-10-27 | 2010-04-21 | 云南省农业科学院花卉研究所 | Tissue culture and rapid propagation method of raspberries |
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