CN110664989A - Striae gravidarum removing composition containing polypeptide and preparation method and application thereof - Google Patents
Striae gravidarum removing composition containing polypeptide and preparation method and application thereof Download PDFInfo
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 42
- 206010040925 Skin striae Diseases 0.000 title claims abstract description 40
- 239000000203 mixture Substances 0.000 title claims abstract description 30
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 23
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 240000006740 Cichorium endivia Species 0.000 claims abstract description 22
- 235000003733 chicria Nutrition 0.000 claims abstract description 22
- IHRKJQSLKLYWBQ-QKDODKLFSA-N (2s)-2-[[(2s)-1-[(2s)-5-amino-2-[[2-(hexadecanoylamino)acetyl]amino]-5-oxopentanoyl]pyrrolidine-2-carbonyl]amino]-5-(diaminomethylideneamino)pentanoic acid Chemical group CCCCCCCCCCCCCCCC(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C(N)N)C(O)=O IHRKJQSLKLYWBQ-QKDODKLFSA-N 0.000 claims abstract description 19
- 229940056582 human hair preparation Drugs 0.000 claims abstract description 19
- 229940092309 pumpkin seed extract Drugs 0.000 claims abstract description 18
- 229940094946 palmitoyl tetrapeptide-7 Drugs 0.000 claims abstract description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 60
- 238000002156 mixing Methods 0.000 claims description 49
- 239000000047 product Substances 0.000 claims description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- 238000001035 drying Methods 0.000 claims description 20
- 239000000706 filtrate Substances 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 18
- 240000001980 Cucurbita pepo Species 0.000 claims description 15
- 235000009852 Cucurbita pepo Nutrition 0.000 claims description 15
- 238000000605 extraction Methods 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 10
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 10
- 239000002244 precipitate Substances 0.000 claims description 10
- 239000011347 resin Substances 0.000 claims description 10
- 229920005989 resin Polymers 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 10
- 239000003480 eluent Substances 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 6
- 229940099259 vaseline Drugs 0.000 claims description 6
- 238000000944 Soxhlet extraction Methods 0.000 claims description 5
- 239000003463 adsorbent Substances 0.000 claims description 5
- 239000000919 ceramic Substances 0.000 claims description 5
- 238000005238 degreasing Methods 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
- 238000010828 elution Methods 0.000 claims description 5
- 150000002195 fatty ethers Chemical class 0.000 claims description 5
- 239000012535 impurity Substances 0.000 claims description 5
- 230000014759 maintenance of location Effects 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 5
- 238000007873 sieving Methods 0.000 claims description 5
- 239000002904 solvent Substances 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- 239000003814 drug Substances 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 3
- 241000219130 Cucurbita pepo subsp. pepo Species 0.000 claims 1
- 235000003954 Cucurbita pepo var melopepo Nutrition 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000002994 raw material Substances 0.000 abstract description 6
- 230000037303 wrinkles Effects 0.000 abstract description 3
- 206010050637 Skin tightness Diseases 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 8
- 238000009499 grossing Methods 0.000 description 8
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- 208000031439 Striae Distensae Diseases 0.000 description 3
- 239000003054 catalyst Substances 0.000 description 3
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- 230000036541 health Effects 0.000 description 2
- 239000003973 paint Substances 0.000 description 2
- 206010000060 Abdominal distension Diseases 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 210000000579 abdominal fat Anatomy 0.000 description 1
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- 238000009825 accumulation Methods 0.000 description 1
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/02—Peptides of undefined number of amino acids; Derivatives thereof
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/36—Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells
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- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/618—Molluscs, e.g. fresh-water molluscs, oysters, clams, squids, octopus, cuttlefish, snails or slugs
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/07—Tetrapeptides
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
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- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/16—Emollients or protectives, e.g. against radiation
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Abstract
The invention discloses a striae gravidarum removing composition containing polypeptide and a preparation method and application thereof. The striae gravidarum removing composition comprises the following components in parts by weight: 6-13 parts of transdermal short peptide, 3-7 parts of palmitoyl tetrapeptide-7 parts, 10-22 parts of pumpkin seed extract, 4-8 parts of crinis carbonisatus, 5-15 parts of cuttlebone and 5-12 parts of endive root extract. The striae gravidarum removing composition is prepared from transdermal short peptide, palmitoyl tetrapeptide-7, pumpkin seed extract, crinis carbonisatus, cuttlebone and endive root extract, the raw materials are safe, the prepared composition cannot cause damage to the body of pregnant women or lying-in women, and the preparation process is simple and easy to implement; by using the striae gravidarum removing composition for 50 days, the wrinkle removing rate can reach over 90 percent, the skin color uniformity reaches 95 percent, and the skin tightness, smoothness and fineness are obviously improved.
Description
Technical Field
The invention relates to a pharmaceutical composition, in particular to a striae gravidarum removing composition containing polypeptide and a preparation method and application thereof.
Background
The striae gravidarum is mainly formed due to the influence of hormones in pregnancy, and the abdominal distension causes elastic fibers and collagen fibers of the skin to be damaged or broken, so that the abdominal skin becomes thin and thin, and pink or purple wavy patterns with different widths and lengths appear. After delivery, these lines gradually disappear, leaving white or silvery-white shiny scar lines, i.e. stretch marks. Stretch marks appear mainly on the abdominal wall, and may also appear on the inside and outside of the thighs, buttocks, chest, back waist, arms, and the like. The striae gravidarum is accompanied by skin relaxation, breast drop and abdominal fat accumulation, which seriously affects the postpartum physical and mental health of women.
At present, some medicines for removing striae gravidarum exist in the market, but pregnant women have babies, and the lying-in women need to nurse the babies after birth, so the medicines are very cautious. More or less striae gravidarum on the market has some chemical components, and when the dosage is more, the body health of pregnant women or lying-in women is influenced, and the body health of infants is influenced; when the dosage is less, the effect of removing striae gravidarum is not obvious.
Disclosure of Invention
The invention aims to provide a striae gravidarum removing composition containing polypeptide, a preparation method and application thereof, and aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a striae gravidarum removing composition containing polypeptide comprises the following components in parts by weight: 6-13 parts of transdermal short peptide, 3-7 parts of palmitoyl tetrapeptide-7 parts, 10-22 parts of pumpkin seed extract, 4-8 parts of crinis carbonisatus, 5-15 parts of cuttlebone and 5-12 parts of endive root extract.
As a further scheme of the invention: the paint comprises the following components in parts by weight: 8-12 parts of transdermal short peptide, 4-6 parts of palmitoyl tetrapeptide-7, 13-20 parts of pumpkin seed extract, 5-7 parts of crinis carbonisatus, 8-11 parts of cuttlebone and 8-10 parts of endive root extract.
As a further scheme of the invention: the paint comprises the following components in parts by weight: 10 parts of transdermal short peptide, 5 parts of palmitoyl tetrapeptide-7, 15 parts of pumpkin seed extract, 6 parts of crinis carbonisatus, 10 parts of cuttlebone and 9 parts of endive root extract.
As a further scheme of the invention: the preparation method of the extract of the radices sophorae flavescentis comprises the following steps: weighing herba Sonchi Oleracei root, decocting with water, cooling the decoction to room temperature, and centrifuging to obtain medicinal liquid and precipitate; microfiltering the medicinal liquid through an inorganic ceramic membrane, and collecting to obtain a microfiltrate; passing the micro filtrate through AB-8 macroporous adsorbent resin column, eluting with 70-75% ethanol, collecting eluate, concentrating the eluate at 60-70 deg.C under reduced pressure, and recovering ethanol to obtain extract I; drying the precipitate under reduced pressure, performing Soxhlet extraction with 70-75% ethanol, and concentrating the extractive solution at 72-75 deg.C under reduced pressure to recover ethanol to obtain extract II; and mixing the extract I and the extract II, uniformly mixing, and drying to obtain the extract of the root of the endive.
As a further scheme of the invention: the preparation method of the pumpkin seed extract comprises the following steps: crushing the cucurbita pepo seeds into 100-200 meshes; degreasing the obtained product with fatty ether or n-hexane at 47-50 deg.C for 2-3 h; filtering, and removing the filtrate to obtain degreased particles; then ethanol with the volume concentration of 65 percent is used as an extraction solvent for extraction, and the ratio of the material to the liquid is 1: 25-30; filtering the extracting solution to obtain an extracting filtrate; adsorbing and separating the extract filtrate with low-polarity or medium-polarity macroporous resin, eluting with distilled water to remove impurities, eluting with 65% ethanol when the effluent is colorless, stopping elution when the volume of the eluate is 8-10 column retention volumes, and mixing the eluates; drying the eluent to obtain the cucurbita pepo seed extract.
A method for preparing striae gravidarum removing composition containing polypeptide comprises the following steps:
(1) mixing the transdermal short peptide and the palmitoyl tetrapeptide-7, adding 25-40 parts of water, and uniformly mixing to obtain a product I;
(2) mixing the carbonized human hair and the cuttlebone, crushing, sieving by a sieve of 100 meshes and 200 meshes, adding 15-20 parts of water, and mixing for 10-20min at 90-98 ℃ to obtain a product II;
(3) mixing Cucurbita pepo seed extract and herba Sonchi arvensis root extract, adding 30-45 parts of water, and mixing at 80-90 deg.C for 20-35min to obtain product III;
(4) and mixing the product I, the product II and the product III, stirring uniformly at normal temperature, drying at 45-55 ℃, adding vaseline, and stirring uniformly to obtain the product.
Application of striae gravidarum removing composition containing polypeptide in preparing striae gravidarum removing medicine is provided.
Compared with the prior art, the invention has the beneficial effects that:
the striae gravidarum removing composition is prepared from transdermal short peptide, palmitoyl tetrapeptide-7, pumpkin seed extract, crinis carbonisatus, cuttlebone and endive root extract, the raw materials are safe, the prepared composition cannot cause damage to the body of pregnant women or lying-in women, and the preparation process is simple and easy to implement; by using the striae gravidarum removing composition for 50 days, the wrinkle removing rate can reach over 90 percent, the skin color uniformity reaches 95 percent, and the skin tightness, smoothness and fineness are obviously improved.
Detailed Description
The technical solution of the present patent will be described in further detail with reference to the following embodiments.
Example 1
A striae gravidarum removing composition containing polypeptide comprises the following components in parts by weight: 6 parts of transdermal short peptide, 3 parts of palmitoyl tetrapeptide-7, 10 parts of pumpkin seed extract, 4 parts of crinis carbonisatus, 5 parts of cuttlebone and 5 parts of endive root extract.
The preparation method of the extract of the radices sophorae flavescentis comprises the following steps: weighing herba Sonchi Oleracei root, decocting with water to obtain medicinal liquid and precipitate; microfiltering the medicinal liquid through an inorganic ceramic membrane, and collecting to obtain a microfiltrate; passing the micro-filtrate through AB-8 macroporous adsorbent resin column, eluting with 70% ethanol, collecting eluate, concentrating the eluate at 60 deg.C under reduced pressure, and recovering ethanol to obtain extract I; drying the precipitate under reduced pressure, performing Soxhlet extraction with 70% ethanol, and concentrating the extractive solution at 72 deg.C under reduced pressure to recover ethanol to obtain extract II; and mixing the extract I and the extract II, uniformly mixing, and drying to obtain the extract of the root of the endive.
The preparation method of the pumpkin seed extract comprises the following steps: crushing the cucurbita pepo seeds into 100 meshes; degreasing the obtained product with fatty ether or n-hexane at 47 deg.C for 2 h; filtering, and removing the filtrate to obtain degreased particles; then ethanol with the volume concentration of 65 percent is used as an extraction solvent for extraction, and the ratio of the material to the liquid is 1: 25; filtering the extracting solution to obtain an extracting filtrate; adsorbing and separating the extracted filtrate by using low-polarity or medium-polarity macroporous resin, eluting with distilled water to remove impurities, eluting with 65% ethanol by volume concentration when the effluent liquid is colorless, stopping elution when the volume of the eluate is 8 column retention volumes, and combining the eluates in the section; drying the eluent to obtain the cucurbita pepo seed extract.
A method for preparing striae gravidarum removing composition containing polypeptide comprises the following steps: (1) mixing the transdermal short peptide and the palmitoyl tetrapeptide-7, adding 25 parts of water, and uniformly mixing to obtain a product I; (2) mixing crinis Carbonisatus and Os Sepiae, pulverizing, sieving with 100 mesh sieve, adding 15 parts of water, and mixing at 90 deg.C for 10min to obtain product II; (3) mixing Cucurbita pepo seed extract and herba Sonchi arvensis root extract, adding 30 parts of water, and mixing at 80 deg.C for 20min to obtain product III; (4) and mixing the product I, the product II and the product III, stirring uniformly at normal temperature, drying at 45 ℃, adding vaseline, and stirring uniformly to obtain the catalyst.
Example 2
A striae gravidarum removing composition containing polypeptide comprises the following components in parts by weight: 13 parts of transdermal short peptide, 7 parts of palmitoyl tetrapeptide-7, 22 parts of pumpkin seed extract, 8 parts of crinis carbonisatus, 15 parts of cuttlebone and 12 parts of endive root extract.
The preparation method of the extract of the radices sophorae flavescentis comprises the following steps: weighing herba Sonchi Oleracei root, decocting with water to obtain medicinal liquid and precipitate; microfiltering the medicinal liquid through an inorganic ceramic membrane, and collecting to obtain a microfiltrate; passing the micro-filtrate through AB-8 macroporous adsorbent resin column, eluting with 75% ethanol, collecting eluate, concentrating the eluate at 70 deg.C under reduced pressure, and recovering ethanol to obtain extract I; drying the precipitate under reduced pressure, performing Soxhlet extraction with 75% ethanol, and concentrating the extractive solution at 75 deg.C under reduced pressure to recover ethanol to obtain extract II; and mixing the extract I and the extract II, uniformly mixing, and drying to obtain the extract of the root of the endive.
The preparation method of the pumpkin seed extract comprises the following steps: crushing the cucurbita pepo seeds into 200 meshes; degreasing the obtained product with fatty ether or n-hexane at 50 deg.C for 3 hr; filtering, and removing the filtrate to obtain degreased particles; then ethanol with the volume concentration of 65 percent is used as an extraction solvent for extraction, and the ratio of the material to the liquid is 1: 30; filtering the extracting solution to obtain an extracting filtrate; adsorbing and separating the extracted filtrate by using low-polarity or medium-polarity macroporous resin, eluting with distilled water to remove impurities, eluting with 65% ethanol by volume concentration when the effluent liquid is colorless, stopping elution when the volume of the eluent is 10 column retention volumes, and combining the eluates in the section; drying the eluent to obtain the cucurbita pepo seed extract.
A method for preparing striae gravidarum removing composition containing polypeptide comprises the following steps: (1) mixing the transdermal short peptide and the palmitoyl tetrapeptide-7, adding 40 parts of water, and uniformly mixing to obtain a product I; (2) mixing crinis Carbonisatus and Os Sepiae, pulverizing, sieving with 200 mesh sieve, adding 20 parts of water, and mixing at 98 deg.C for 20min to obtain product II; (3) mixing Cucurbita pepo seed extract and herba Sonchi arvensis root extract, adding 45 parts of water, and mixing at 90 deg.C for 35min to obtain product III; (4) and mixing the product I, the product II and the product III, stirring uniformly at normal temperature, drying at 55 ℃, adding vaseline, and stirring uniformly to obtain the catalyst.
Example 3
A striae gravidarum removing composition containing polypeptide comprises the following components in parts by weight: 10 parts of transdermal short peptide, 5 parts of palmitoyl tetrapeptide-7, 15 parts of pumpkin seed extract, 6 parts of crinis carbonisatus, 10 parts of cuttlebone and 9 parts of endive root extract.
The preparation method of the extract of the radices sophorae flavescentis comprises the following steps: weighing herba Sonchi Oleracei root, decocting with water, cooling the decoction to room temperature, and centrifuging to obtain medicinal liquid and precipitate; microfiltering the medicinal liquid through an inorganic ceramic membrane, and collecting to obtain a microfiltrate; passing the micro-filtrate through AB-8 macroporous adsorbent resin column, eluting with 72% ethanol, collecting eluate, concentrating the eluate at 65 deg.C under reduced pressure, and recovering ethanol to obtain extract I; drying the precipitate under reduced pressure, performing Soxhlet extraction with 73% ethanol, and concentrating the extractive solution at 73 deg.C under reduced pressure to recover ethanol to obtain extract II; and mixing the extract I and the extract II, uniformly mixing, and drying to obtain the extract of the root of the endive.
The preparation method of the pumpkin seed extract comprises the following steps: crushing the cucurbita pepo seeds into 150 meshes; degreasing the obtained product with fatty ether or n-hexane at 48 deg.C for 2.6 h; filtering, and removing the filtrate to obtain degreased particles; then ethanol with the volume concentration of 65 percent is used as an extraction solvent for extraction, and the ratio of the material to the liquid is 1: 27; filtering the extracting solution to obtain an extracting filtrate; adsorbing and separating the extracted filtrate by using low-polarity or medium-polarity macroporous resin, eluting with distilled water to remove impurities, eluting with 65% ethanol by volume concentration when the effluent liquid is colorless, stopping elution when the volume of the eluent is 9 column retention volumes, and combining the eluates in the section; drying the eluent to obtain the cucurbita pepo seed extract.
A method for preparing striae gravidarum removing composition containing polypeptide comprises the following steps: (1) mixing the transdermal short peptide and the palmitoyl tetrapeptide-7, adding 30 parts of water, and uniformly mixing to obtain a product I; (2) mixing crinis Carbonisatus and Os Sepiae, pulverizing, sieving with 150 mesh sieve, adding 18 parts of water, and mixing at 95 deg.C for 15min to obtain product II; (3) mixing Cucurbita pepo seed extract and herba Sonchi arvensis root extract, adding 35 parts of water, and mixing at 85 deg.C for 25min to obtain product III; (4) and mixing the product I, the product II and the product III, uniformly stirring at normal temperature, drying at 50 ℃, adding vaseline, and uniformly stirring to obtain the catalyst.
Example 4
Different from the embodiment 3, the striae gravidarum removing composition containing the polypeptide comprises the following components in parts by weight: 10 parts of transdermal short peptide, 5 parts of palmitoyl tetrapeptide-7, 15 parts of pumpkin seed extract, 6 parts of crinis carbonisatus, 10 parts of cuttlebone and 9 parts of endive root extract.
Example 5
Different from the embodiment 3, the striae gravidarum removing composition containing the polypeptide comprises the following components in parts by weight: 10 parts of transdermal short peptide, 5 parts of palmitoyl tetrapeptide-7, 15 parts of pumpkin seed extract, 6 parts of crinis carbonisatus, 10 parts of cuttlebone and 9 parts of endive root extract.
Comparative example 1
The preparation process is the same as example 3 except that the raw materials do not contain crinis carbonisatus.
Comparative example 2
The preparation process is identical to example 3, except that the raw material does not contain the extract of the endive root.
Comparative example 3
The preparation process is identical to example 3, except that the raw materials do not contain crinis carbonisatus and extract of endive root.
Comparative example 4
The raw materials are consistent with those in example 3, and the preparation process adopts: mixing all the materials, and mixing with vaseline.
Examples of the experiments
800 patients with stretch marks are selected and divided into 8 groups, and examples 1-3, comparative examples 1-4 and commercial repair cream (control group) are respectively used; applying 5g of the ointment to the affected part every day, eliminating striae gravidarum, and stopping applying after 50 days, and counting the cure rate, and after 1 year, counting the recurrence rate. The statistical results are shown in table 1:
TABLE 1
| Item | The striae gravidarum removing rate% | The color and luster of the skin are uniform | Skin condition | Relapse rate after 1 year% |
| Example 1 | 92 | 95 | Compacting; smoothing; fineness of fineness | 1 |
| Example 2 | 91 | 96 | Compacting; smoothing; fineness of fineness | 1 |
| Example 3 | 95 | 98 | Compacting; smoothing; fineness of fineness | 0 |
| Comparative example 1 | 64 | 80 | The structure is compact; smoothing; fineness of fineness | 6 |
| Comparative example 2 | 72 | 68 | Not compact; smoothing; fineness of fineness | 5 |
| Comparative example 3 | 62 | 65 | Not compact; smoothing; fineness of fineness | 8 |
| Comparison ofExample 4 | 80 | 82 | Compacting; smoothing; fineness of fineness | 3 |
| Control group | 87 | 90 | Compacting; smoothing; fineness of fineness | 7 |
The result shows that the product of the invention can remove wrinkles by more than 90 percent in 50 days, the color uniformity of the skin reaches 95 percent, and the skin is firmed, smooth and fine.
Although the preferred embodiments of the present patent have been described in detail, the present patent is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present patent within the knowledge of those skilled in the art.
Claims (7)
1. The striae gravidarum removing composition containing the polypeptide is characterized by comprising the following components in parts by weight: 6-13 parts of transdermal short peptide, 3-7 parts of palmitoyl tetrapeptide-7 parts, 10-22 parts of pumpkin seed extract, 4-8 parts of crinis carbonisatus, 5-15 parts of cuttlebone and 5-12 parts of endive root extract.
2. The striae gravidarum removing composition containing the polypeptide according to claim 1, which comprises the following components in parts by weight: 8-12 parts of transdermal short peptide, 4-6 parts of palmitoyl tetrapeptide-7, 13-20 parts of pumpkin seed extract, 5-7 parts of crinis carbonisatus, 8-11 parts of cuttlebone and 8-10 parts of endive root extract.
3. The striae gravidarum removing composition containing the polypeptide according to claim 1, which comprises the following components in parts by weight: 10 parts of transdermal short peptide, 5 parts of palmitoyl tetrapeptide-7, 15 parts of pumpkin seed extract, 6 parts of crinis carbonisatus, 10 parts of cuttlebone and 9 parts of endive root extract.
4. The striae gravidarum removing composition containing polypeptides according to claim 1, wherein the extract of endive root is prepared by: weighing herba Sonchi Oleracei root, decocting with water, cooling the decoction to room temperature, and centrifuging to obtain medicinal liquid and precipitate; microfiltering the medicinal liquid through an inorganic ceramic membrane, and collecting to obtain a microfiltrate; passing the micro filtrate through AB-8 macroporous adsorbent resin column, eluting with 70-75% ethanol, collecting eluate, concentrating the eluate at 60-70 deg.C under reduced pressure, and recovering ethanol to obtain extract I; drying the precipitate under reduced pressure, performing Soxhlet extraction with 70-75% ethanol, and concentrating the extractive solution at 72-75 deg.C under reduced pressure to recover ethanol to obtain extract II; and mixing the extract I and the extract II, uniformly mixing, and drying to obtain the extract of the root of the endive.
5. The striae gravidarum removing composition containing the polypeptide according to claim 1, wherein the zucchini seed extract is prepared by: crushing the cucurbita pepo seeds into 100-200 meshes; degreasing the obtained product with fatty ether or n-hexane at 47-50 deg.C for 2-3 h; filtering, and removing the filtrate to obtain degreased particles; then ethanol with the volume concentration of 65 percent is used as an extraction solvent for extraction, and the ratio of the material to the liquid is 1: 25-30; filtering the extracting solution to obtain an extracting filtrate; adsorbing and separating the extract filtrate with low-polarity or medium-polarity macroporous resin, eluting with distilled water to remove impurities, eluting with 65% ethanol when the effluent is colorless, stopping elution when the volume of the eluate is 8-10 column retention volumes, and mixing the eluates; drying the eluent to obtain the cucurbita pepo seed extract.
6. A process for preparing a striae gravidarum removing composition containing a polypeptide according to any of claims 1-5, comprising the steps of:
(1) mixing the transdermal short peptide and the palmitoyl tetrapeptide-7, adding 25-40 parts of water, and uniformly mixing to obtain a product I;
(2) mixing the carbonized human hair and the cuttlebone, crushing, sieving by a sieve of 100 meshes and 200 meshes, adding 15-20 parts of water, and mixing for 10-20min at 90-98 ℃ to obtain a product II;
(3) mixing Cucurbita pepo seed extract and herba Sonchi arvensis root extract, adding 30-45 parts of water, and mixing at 80-90 deg.C for 20-35min to obtain product III;
(4) and mixing the product I, the product II and the product III, stirring uniformly at normal temperature, drying at 45-55 ℃, adding vaseline, and stirring uniformly to obtain the product.
7. Use of a striae gravidarum removing composition containing a polypeptide according to any one of claims 1-5 in the preparation of a striae gravidarum removing medicament.
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