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CN110628822A - 重配btv和ahsv疫苗 - Google Patents

重配btv和ahsv疫苗 Download PDF

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CN110628822A
CN110628822A CN201910804847.8A CN201910804847A CN110628822A CN 110628822 A CN110628822 A CN 110628822A CN 201910804847 A CN201910804847 A CN 201910804847A CN 110628822 A CN110628822 A CN 110628822A
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M·帕尔马里尼
P·于代莱
J-C·奥多内
S·F·努涅斯
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Merial Ltd
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Abstract

本发明包括BTV和ASHV疫苗或产生重组重配BTV和ASHV载体的组合物和方法，以及接种抗BTV和ASHV的疫苗的方法。

Description

重配BTV和AHSV疫苗
本申请是2013年6月13日提交的同名发明专利申请201380038286.6的分案申请。
【相关申请的交叉参考】
本申请要求2012年6月13日提交的美国临时申请61/659,198的优先权。
【技术领域】
本发明涉及用于抵抗动物的蓝舌病病毒(Bluetongue Virus)(BTV)或非洲马瘟病毒(African Horse Sickness Virus)(AHSV)感染的组合物。本发明提供了包括重组的BTV或AHSV载体的药物组合物，接种抗BTV或AHSV疫苗的方法以及用于与这样的方法和组合物使用的试剂盒。
【背景技术】
蓝舌病(BT)是反刍动物的节肢动物传播的感染性病毒疾病。牛和山羊可容易地感染致病性BTV但没有广泛的血管损伤，从而这些物种通常不能显示明显的临床体征。相反地，该疾病在羊中的特征在于口、鼻和前胃的粘膜的卡他性炎症，和蹄的冠状带和蹄叶的炎症。存在上皮的擦伤，和颊粘膜的最终坏死；肿胀发炎的舌和口呈现蓝色，因而疾病据此命名(Spreull1905)。在绵羊中的死亡率被估计在1－30％。
BTV是环状病毒属(Orbivirus genus)(呼肠弧病毒科(Reoviridae family))的原型病毒，其由至少24种不同血清型(Wilson和Mecham 2000)组成。BTV的不同菌株在世界范围内已被鉴定为遍布整个热带和温带地区。BTV感染的发生在欧洲远至45°N，在亚洲和北美远至50°N，以及向南远至35°。BTV不在反刍动物之间接触传染，因此BTV的分布依赖于库蠓属(Culicoides sp.)的节肢动物媒介种类(vector species)(咬蠓(biting midges))的存在，不同的媒介种类存在于世界的不同地区。最近的数据表明，遗传漂变和沉默效应(founder effect)促成BTV的野生株的单个基因区段的多样化(Bonneau,Mullens等人，2001)。已经显示BTV血清阳性动物对同源BTV血清型的感染具有抗性。
反刍动物的BTV感染是瞬时的，而库蠓属(Culicoides)昆虫载体的感染是持久的。病毒血症的持续时间取决于动物种类和BTV的毒株。据报道，病毒血症在绵羊中可以是非常短暂的，在BTV感染的个体中可持续长达41天，在山羊中长达42天，在牛中长达100天。由于牛的BTV感染通常导致长时间，但不持久的病毒血症，因此牛充当库蠓属媒介可从其摄取病毒，随后传播给其它反刍动物的贮库(Anderson,Stott等人，1985；MacLachlan 1994；MacLachlan和Pearson 2004)。库蠓属媒介的许多种类的生态学知之甚少并且它们的孳生地在很大程度上未被鉴定，它们的扩散率是未知的。黑唇库蠓(Culicoides sonorensis)是BTV在北美的主要载体。雌库蠓昆虫成为被BTV持续感染和并且在长达14天的外潜伏期后能传播病毒(Mullens，Tabachnick等，1995)。BTV在温带地区越冬可通过垂直感染的昆虫媒介实现，但最近的数据表明，在持续BTV感染过程中在昆虫媒介的幼虫阶段外衣壳基因表达减少(White,Wilson等人2005)。
BTV的病毒体具有为～69nm的直径，双壳外套(衣壳)有时被从受感染细胞的细胞膜衍生的脂蛋白“假包膜”包围。BTV基因组包括10个不同的双链RNA片段，其共同地编码7个结构(VP1至VP7)和4个非结构(NS1、NS2、NS3和NS3a)蛋白(Roy 1996)；基因组片段中有9个是单顺反子，而区段10使用第二框内起始密码子编码NS3和NS3A。基因组RNA在二十面体病毒体颗粒中被双层蛋白质衣壳包壳(Verwoerd,Els等人1972)。二十面体核心由2个大蛋白质(VP3和VP7)和3个小的蛋白质(VP1、VP4、VP6)组成并且被外衣壳包围，所述外衣壳由分别由基因组区段2和5编码的VP2和VP5组成(Roy 1996)。VP2负责BTV的结合和进入细胞、中和、血清型特异性和血凝集。VP2的多聚体形式(二聚体和三聚体)装饰病毒颗粒外表面上的大部分VP5支架表面(Hassan和Roy 1999)。VP2在24种BTV血清型中变化最大，并且抗VP2抗体的水平与体外和体内病毒中和相关(Huismans和Erasmus 1981)。VP5在BTV的不同血清型和毒株之间变化也很明显(de Mattos,de Mattos等人，1994；DeMaula,Bonneau等人2000)，虽然迄今还未鉴定出VP5特异性中和MAb，但数据表明，这种蛋白质通过其对VP2的构象影响在中和以及血清型测定中具有作用(Huismans和Erasmus 1981；Roy,Urakawa等人1990；DeMaula等人,2000)。将利用BTV抗核心血清免疫吸附以除去痕量VP7的纯化的VP2注射入绵羊。50微克的VP2的初始剂量足以诱导VP2-沉淀抗体以及中和和血凝抑制抗体。这些绵羊针对利用相同BTV血清型的毒株的攻击得到了充分保护。即便在攻击前未检测到中和抗体，较低剂量的VP2仍然提供显著水平的保护(Huismans,van der Walt等人1987)。最近的结果表明，VP2和NS1表达被细胞毒性T淋巴细胞(CTL)识别的表位(Andrew,Whiteley等人1995)，然而VP7和VP5不可能具有CTL表位。到目前为止，VP3、VP4、VP6、NS2和NS3没有刺激绵羊的CTL反应(Lobato,Coupar等人1997)。下面的表1(从Wilson和Mecham 2000修改而来)概述了BTV的基因及其蛋白质的功能。
表1蓝舌病病毒基因和具有蛋白的定位、性质和功能的编码的蛋白
感兴趣的特定BTV抗原多肽包括VP2和VP5。二十面体核心由两种大(VP3和VP7)和三种小的蛋白质(VP1、VP4、VP6)组成，并且被外衣壳包围，所述外衣壳被分别由基因组区段2和5编码的VP2和VP5包围(Roy 1996)。VP2负责BTV的结合和进入细胞、中和、血清型特异性和血凝集。VP2的多聚体形式(二聚体和三聚体)装饰病毒颗粒外表面上的VP5支架的大部分表面(Hassan和Roy 1999)。VP2在24种BTV血清型中变化最大，抗VP2抗体的水平与体外和体内病毒中和相关(Huismans和Erasmus 1981)。VP5在BTV的不同血清型和毒株之间变化也很明显(de Mattos,de Mattos等人，1994；DeMaula,Bonneau等人2000)，虽然迄今还未鉴定出VP5特异性中和MAb，但数据表明，这种蛋白通过其对VP2的构象影响在中和以及血清型测定中具有作用(Huismans和Erasmus 1981；Roy,Urakawa等人1990；DeMaula等人,2000)。将利用BTV抗核心血清免疫吸附以除去痕量VP7的纯化的VP2注射入绵羊。50微克的VP2的初始剂量足以诱导VP2-沉淀抗体以及中和和血凝抑制抗体。这些绵羊针对利用相同BTV血清型的毒株的攻击得到了充分保护。即便在攻击前未检测到中和抗体，较低剂量的VP2仍然提供显著水平的保护(Huismans,van der Walt等人1987)。最近的结果表明，VP2和NS1表达被细胞毒性T淋巴细胞(CTL)识别的表位(Andrew,Whiteley等人1995)，然而VP7和VP5不可能具有CTL表位。到目前为止，VP3、VP4、VP6、NS2和NS3没有刺激绵羊的CTL反应(Lobato,Coupar等人1997)。参见表1。
非洲马瘟(AHS)是马和骡的严重的、往往是致命的节肢动物传播的病毒病(African Horse Sickness,The Merck Veterinary Manual)。在该疾病的一些形式中死亡率可高达95％。无症状或轻度感染可以在马以及斑马和驴，特别是在先前感染了不同血清型的病毒的马中发生。受感染的动物或媒介可携带病毒至无AHS的地区。有些学者推测，气候变化可能会增加节肢动物传播的疾病，如非洲马瘟蔓延的风险，因为最近已出现相关蓝舌病病毒(Wilson A等人,Parasitol.Res.2008；103:69–77)。残肢库蠓(Culicoidesimicola)，该疾病的主要媒介，已侵入北非和南欧。潜在的节肢动物媒介事实上也遍及全球几乎所有地区，包括美国的许多地区和美洲其余地区。
非洲马瘟起因于感染非洲马瘟病毒(呼肠病毒科的环状病毒的成员)。迄今为止，非洲马瘟病毒的9个血清型是已知的。非洲马瘟病毒血清型9遍布流行地区，然而血清型1至8主要见于有限的地理区域。血清型9一直是造成大部分非洲以外的非洲马瘟暴发的致病原因。血清型4于1987年与1990年之间在西班牙和葡萄牙造成暴发(Lubroth J.,EquinePract.1988；10:26–33)。
关于非洲马瘟病毒的初步研究导致十九世纪三十年代针对非洲马瘟病毒的小鼠脑减毒修饰的活病毒疫苗的开发。这些疫苗被精制，并导致十九世纪六十年代组织培养减毒修饰的活病毒(MLV)疫苗的开发。
尽管该疫苗具有功效，但其具有一些固有的限制，包括个别动物的疫苗反应(包括死亡)，个体动物的变化多样的免疫反应，在通过被动母体免疫来免疫幼小动物中的困难，疫苗病毒的毒力的恢复的可能性，以及接种后疫苗株的重组和可能的毒力恢复(duPlessis M.等人，1998,Onderstepoort Journal of Veterinary Research 65:321-329)。还存在关于使用MLV疫苗的社会经济影响。南非有允许其出口马至欧盟和许多其它国家的流程。该流程也使得来自其它国家的马进入南非以在各活动中竞赛或临时作为种畜的可能。流程基于确保马对抗非洲马瘟病毒充分疫苗接种。兽医局知道使用MLV疫苗的可能的危险。这些问题的大部分将通过开发替代性非洲马瘟病毒疫苗大大降低。
非洲马瘟病毒基因组由编码至少10种病毒蛋白的10个双链RNA区段(Oellermann,R.A.等人，1970；Bremer,C.W.等人，1976)组成。该基因组区段按它们在PAGE中迁移的顺序编号为1-10。病毒蛋白中有7种是结构性的并且形成双壳病毒颗粒。外衣壳是由两种大病毒蛋白VP2和VP5组成，所述病毒蛋白决定非洲马瘟病毒的抗原变异性，而内衣壳由两种大的(VP3和VP7)和三种小的(VP1、VP4和VP6)病毒蛋白组成(Lewis SA和Grubman MJ,1991；Martinez-Torrecuadrada JL等人,1994)；Bremer,CW等人，1990；Grubman,M.J.&Lewis,S.A.,1992)。VP3和VP7在9种血清型中是高度保守的(Oellermann等人，1970；Bremer等人，1990)。至少三个非结构蛋白NS1、NS2和NS3已被鉴定(Huismans,H.&Els,H.J.,1979；vanStaden,V.&Huismans,H.,1991；Mizukoshi,N.等人,1992)。
来源于减毒病毒的重组金丝雀痘病毒已被开发为用于异源病毒基因表达的载体。其中一些金丝雀痘构建至今已在许多国家包括南非、欧盟和美利坚合众国被授权作为在马(Minke JM，等人，2004a和b；Minke JM，等人，2007；Siger L，等人，2006)和其它物种(Poulet H，等人，2003)中使用的疫苗。
这些疫苗仅含有目标生物的基因的事实使得它们固有地安全(Minke JM，等人，2004b)。此外，即使在单次剂量的疫苗施用后，可检测的中和抗体的出现仍然是快速的(Minke JM等人，2004b)。这样的疫苗的固有安全性和中和抗体的产生的性质使得这样的疫苗在动物流行病中的使用特别具有吸引力(Minke JM等人，2004a)。
先前的研究已经表明，当用外源表达的VP2和适当的佐剂接种马时，它们产生针对AHS的中和抗体(Scanlen M，等人，2002年)。绵羊中的研究显示，针对蓝舌病病毒的中和抗体反应通过用其中共表达VP2和VP5的病毒样颗粒接种绵羊得到增强(Pearson LD,Roy P,1993)。最近已显示，共表达为编码蓝舌病病毒的VP2和VP5外衣壳蛋白的基因的重组金丝雀痘病毒疫苗在绵羊中诱导高水平的保护作用(Boone JD等人，2007)。
还未证明的是，当用含有AHSV VP2和VP5并共表达其的载体接种时，马产生针对非洲马瘟病毒的中和抗体。因此，可以理解的是，本发明通过提供重组痘病毒，包括来源于其的组合物和产品，特别是基于ALVAC的重组体和来源于其的组合物和产品，尤其是这样的表达AHSV VP 2和5的重组体或其任意组合和来源于其的组合物和产品，满足了本领域内的需要。
关于BTV或AHSV的遗传修饰的研究已经由Piet A van Rijn等人(VirologyJournal,2010,7:261)、Polly Roy等人(Journal of Viology,2011,85,19；10213-10221)、Polly Roy等人(Journal of Viology,2008,p8339-8348)、Massimo Palmarini等人(PloSpathogens,2011,7(12):e1002477)以及在WO2009/068870中进行了描述。
因此，有利地提供抗BTV和AHSV的具有改善的免疫原性的疫苗组合物，以及制备和使用这样的组合物(包括提供鉴别诊断法、测定和试剂盒的这样的组合物)的方法。
考虑到动物(包括人)对BTV或AHSV的易感性，预防BTV或AHSV感染和保护动物的方法是必要的。因此，存在对抗BTV或AHSV的有效疫苗的需要。
【发明内容】
提供了包含一种或多种重组重配(reassortant)BTV或AHSV载体的组合物，所述载体包含编码BTV或AHSV的至少一种抗原的一个或多个异源多核苷酸。
本发明的方法包括用于制备该重组重配BTV或AHSV组合物或载体的方法。方法还包括使用方法，所述使用方法包括给动物施用有效量的组合物或载体，以产生保护性免疫原性反应。
【附图说明】
可最好结合附图理解以举例的方式给出，但并非意在仅将本发明限制于所描述的具体实施方案中的下列详细描述，其中：
图1描述了重配BTV-1及BTV-8的示意图和产生重组重配BTV载体的流程图。
图2描述了BTV重配株(reassortant)的平均噬菌斑(plaue)直径。
图3描述了重组病毒的生长曲线分析。大多数重配株与野生型亲代病毒类似地生长。
图4描述了BTV中和结果。
图5描述了BTV重配株的产生。
图6描述了使用反向遗传学(reverse genetics)产生BTV重配株的示意图。
图7显示了包含SEQ ID NO分配以及DNA和蛋白质序列的表。
图8描述了攻击后平均直肠温度的评估。
图9描述了最大过热的分散。
图10描述了平均日临床评分的评估。
图11描述了整体临床评分的分散。
图12描述了平均病毒血症滴度的演变。
图13描述了AUC的分散。
图14描述了均值BTV-8中和抗体滴度的演变。
图15描述了包含每个血清型的VP2和VP5蛋白的sBTV的噬菌斑形态学。
图16描述了BHK-21中的sBTV病毒的病毒滴度。
图17描述了BTV1+BTV8 VP2(RAS10)的感染滴度、ELISA、斑点印迹Vp2。
图18描述了BTV1+BTV8 VP2/VP5(RAS32)的感染滴度、ELISA，斑点印迹Vp2。
【发明详述】
提供包含一种或多种重组BTV或AHSV载体的组合物，所述载体包含编码BTV或AHSV的至少一种抗原的一个或多个异源多核苷酸，所述抗原引发动物的免疫原性反应。在一个实施方案中，多肽抗原是BTV VP2或VP5多肽或其活性片段或变体。
应当认识到，本发明的抗原性多肽可以是全长多肽或其活性片段或变体。“活性片段”或“活性变体”是指片段或变体保留了多肽的抗原性质。因此，本发明包括引发动物的免疫原性反应的任何BTV或AHSV多肽、抗原、表位或免疫原。所述BTV或AHSV多肽、抗原、表位或免疫原可以是任何BTV或AHSV多肽、抗原、表位或免疫原，例如但不限于，引发、诱导或刺激动物例如绵羊类动物、牛类动物、山羊类动物或马类动物的反应的蛋白质、肽或其片段或变体。
本发明涉及牛类动物、绵羊类动物、山羊类动物或马类动物疫苗或组合物，其可包含有效量的重组BTV或AHSV载体和药学上或兽医学上可接受的载体、赋形剂、佐剂或媒介物。
在一些实施方案中，所述疫苗还包含佐剂，例如美国专利7,371,395中描述的水包油(O/W)乳剂。
在其它实施方案中，佐剂包括EMULSIGEN、氢氧化铝和皂苷和CpG，或其组合。
在一些实施方案中，动物的反应是保护性免疫反应。
关于“动物”，其意指哺乳动物、鸟类等。动物或宿主包括哺乳动物和人类。动物可选自马科动物(equine)(例如，马)、犬科动物(cannine)(例如，狗、狼、狐狸、丛林狼、豺)、猫科动物(feline)(例如，狮子、老虎、家猫、野猫、其它大型猫科动物和其它猫科动物，包括猎豹和山猫)、绵羊类动物(ovine)(例如绵羊)、牛类动物(bovine)(例如，牛)、猪类动物(porcine)(例如，猪)、山羊类动物(caprine)(例如，山羊)、禽类动物(avian)(例如，鸡、鸭、鹅、火鸡、鹌鹑、雉鸡、鹦鹉、雀、鹰、乌鸦、鸵鸟、鸸鹋和鹤鸵)、灵长类动物(primate)(例如，狐猴、眼镜猴、猴、长臂猿、猿)和鱼。术语“动物”还包括所有发育阶段，包括胚胎期和胎儿期中的个体动物。
除非另有说明，否则本文使用的所有技术和科学术语具有与本公开内容所属的技术领域的普通技术人员通常中理解的含义相同的含义。除非上下文另外明确指出，否则单数术语“一个/一种(a)”、“一个/一种(an)”和“该/所述/其(the)”包括复数对象。类似地，除非上下文清楚地表明并非如此，否则单词“或”旨在包括“和”。
应当指出，在本公开和特别在权利要求和/或段落中，术语例如“包含(comprises)”、“包含(comprised)”、“包含(comprising)”等可具有美国专利法中规定的含义；例如，它们可以意指“包括(includes)”、“包括(included)”、“包括(including)”等；并且术语例如“基本上由......组成(consisting essentially of)”和“基本上由……组成(consists essentially of)”具有美国专利法中规定的含义，例如，它们允许未明确引述的元素，但不包括在现有技术中被发现的要素或影响本发明的基本或新颖特征的元素。
术语“重组BTV或AHSV载体”、“重组重配BTV或AHSV载体”、“重配BTV或AHSV”、“BTV或AHSV重配株”在本文可互换使用，是指BTV或AHSV病毒的任何修饰、改变或工程。BTV或AHSV病毒的修饰、改变或工程可包括但不限于一个或多个核苷酸或氨基酸的缺失，整个基因的缺失，基因的密码子优化，氨基酸的保守置换，一个或多个异源多核苷酸的插入，不同血清型或物种的基因、转录物、RNA区段，DNA区段至新组合的混合。
本发明的抗原性多肽能够防止BTV和AHSV的侵害。也就是说，它们能够刺激动物的免疫反应。“抗原”或“免疫原”是指诱导宿主动物特异性免疫反应的物质。抗原可以包括整个生物体(杀死的、减毒的或活的)；生物体的亚单位或部分；包含具有免疫原性性质的插入的重组载体；在呈递给宿主动物后能够诱导免疫反应的DNA的碎片或片段；多肽、表位、半抗原或其任意组合。或者，免疫原或抗原可包含毒素或抗毒素。
如本文所用，术语“免疫原性蛋白、多肽或肽”包括这样的多肽，所述多肽在一旦给宿主施用后，其就能够激起针对所述蛋白的体液和/或细胞类型的免疫反应的意义上是有免疫活性的。优选地蛋白片段是这样的片段，其具有与完整蛋白大体上相同的免疫活性。因此，根据本发明的蛋白片段包含至少一个表位或抗原决定簇，或基本上由其组成或由其组成。如本文所用，“免疫原性”蛋白或多肽，包括蛋白的全长序列，其类似物，或其免疫原性片段。“免疫原性片段”意指包含一个或多个表位，从而引发上述免疫反应的蛋白的片段。这样的片段可使用任何数目的本领域中公知的表位作图技术来鉴定。参见，例如，EpitopeMapping Protocols in Methods in Molecular Biology,第66卷(Glenn E.Morris,编辑,1996)。例如，线性表位可以通过例如如下过程来确定：在固体载体上同时合成大量肽，所述肽对应于蛋白质分子的各个部分，随后将肽与抗体反应，同时肽仍然附连于载体。这样的技术在本领域是已知的并且描述于例如美国专利No.4,708,871；Geysen等人，1984；Geysen等人，1986中。类似地，构象表位可以容易地通过确定氨基酸的空间构象(例如通过例如X射线结晶学和二维核磁共振)来鉴定。参见，例如，Epitope Mapping Protocols，同上。
如所讨论，本发明包括抗原性多肽的活性片段和变体。因此，术语“免疫原性蛋白、多肽或肽”还考虑针对序列的缺失、添加和置换，只要所述多肽起着产生本文中定义的免疫反应的功能即可。术语“保守变异”意指氨基酸残基被另一个生物学上相似的残基置换，或核酸序列中使得所编码的氨基酸残基不发生变化或为另一种生物学上相似的残基的核苷酸的置换。在这方面，特别优选的置换在性质上通常是保守性的，即，在氨基酸的家族内发生的那些置换。例如，氨基酸一般被分成4个家族：(1)酸性-天冬氨酸和谷氨酸；(2)碱性-赖氨酸、精氨酸、组氨酸；(3)非极性-丙氨酸，缬氨酸，亮氨酸，异亮氨酸，脯氨酸，苯丙氨酸，甲硫氨酸，色氨酸；和(4)不带电荷的极性-甘氨酸，天冬酰胺，谷氨酰胺，半胱氨酸，丝氨酸，苏氨酸，酪氨酸。苯丙氨酸，色氨酸和酪氨酸有时被分类为芳香族氨基酸。保守变异的实例包括一个疏水残基如异亮氨酸，缬氨酸，亮氨酸或甲硫氨酸对另一个疏水残基的置换，或一个极性残基对另一个极性残基的置换，例如精氨酸对赖氨酸的置换，谷氨酸对天冬氨酸的置换，或谷氨酰胺对天冬酰胺的置换等；或对生物活性无重大影响的结构上相关的氨基酸对氨基酸的相似保守置换。因此，具有与参考分子大体上相同的氨基酸序列但具有少量大体上不影响该蛋白的免疫原性的氨基酸置换的蛋白质，在参考多肽的定义内。通过这些修饰产生的所有多肽都包括在本文中。术语“保守变异”还包括使用置换的氨基酸替代未置换的亲代氨基酸，只要针对置换的多肽产生的抗体也与未置换的多肽免疫反应。
术语“表位”是指特异性B细胞和/或T细胞对其作出反应的抗原或半抗原上的位点。也可将该术语与“抗原决定簇”或“抗原决定位点”交换使用。可在简单的免疫测定中鉴定识别相同表位的抗体，所述免疫测定显示一种抗体阻断另一种抗体对靶抗原的结合的能力。
针对组合物或疫苗的“免疫反应”是细胞和/或抗体介导的针对目标组合物或疫苗的免疫反应在宿主中的发展。通常地，“免疫反应”包括但不限于下列效应的一个或多个：特异性针对目标组合物或疫苗中包含的一种或多种抗原的抗体、B细胞、辅助T细胞和/或细胞毒性T细胞的产生。优选地，宿主将显示治疗性或保护性免疫反应，以便对新感染的抗性将得到增强和/或疾病的临床严重度减轻。这样的保护作用将通过通常由受感染的宿主显示的症状的减轻或不存在、更快的恢复时间和/或受感染的宿主中更低的病毒滴度来证明。
合成抗原也包括在定义内，例如，多表位、侧翼表位(flanking epitopes)和其它重组或合成衍生的抗原。参见，例如，Bergmann等人，1993；Bergmann等人,1996；Suhrbier,1997；Gardner等人,1998。为了本发明的目的，通常免疫原性片段会包含分子的至少约3个氨基酸，至少约5个氨基酸，至少约10-15个氨基酸，或约15-25个氨基酸或更多个氨基酸。对于片段的长度没有严格的上限，所述片段可包括几乎全长蛋白质序列，或甚至融合蛋白包含所述蛋白的至少一个表位。
因此，表达表位的多核苷酸的最小结构是，其包含编码BTV或AHSV多肽的表位或抗原决定簇的核苷酸或基本上由所述核苷酸组成或由所述核苷酸组成。编码BTV或AHSV多肽的片段的多核苷酸可包含编码多肽的序列的最少15个核苷酸、约30-45个核苷酸、约45-75或至少57、87或150个连续或邻接的核苷酸，或基本上由所述核苷酸组成或由所述核苷酸组成。表位测定法，例如产生重叠肽文库(Hemmer等人,1998)、肽扫描(Geysen等人,1984；Geysen等人,1985；Van der Zee R.等人,1989；Geysen,1990；Multipin.RTM.PeptideSynthesis Kits de Chiron)和算法(De Groot等人,1999；PCT/US2004/022605)可在本发明的实践中使用。
术语“核酸”和“多核苷酸”是指线性或分支的、单或双链的RNA或DNA，或其杂合体。术语还包括RNA/DNA杂合体。以下为多核苷酸的非限制性实例：基因或基因片段、外显子、内含子、mRNA、tRNA、rRNA、核酶、cDNA、重组多核苷酸、分支多核苷酸、质粒、载体、任意序列的分离的DNA、任意序列的分离的RNA、核酸探针和引物。多核苷酸可包含修饰核苷酸，例如甲基化核苷酸和核苷酸类似物、尿嘧啶、其它糖和连接基团例如氟代核酸和硫醇盐，以及核苷酸分支。可聚合后用标记组分进一步修饰核苷酸的序列，例如通过缀合。包括在此定义中的其它类型的修饰是帽、用类似物对1个或更多个天然存在的核苷酸的置换，和装置(means)的引入(用以将多核苷酸连接于蛋白质、金属离子、标记组分、其它多核苷酸或固体载体)。多核苷酸可以通过化学合成获得或来源于微生物。
术语“基因”在广义上用于指与生物学功能相关的多核苷酸的任何区段。因此，基因包括内含子和外显子(如在基因组序列中)，或仅编码序列(如在cDNA中)和/或它们的表达所需的调控序列。例如，基因还指表达mRNA或功能性RNA，或编码特定蛋白质的核酸片段，其包括调控序列。
术语“蛋白质”、“肽”、“多肽”和“多肽片段”在本文可互换使用，是指任何长度的氨基酸残基的聚合物。聚合物可以是线性的或分支的，其可以包含修饰氨基酸或氨基酸类似物，并且其可被除氨基酸外的化学部分中断。术语还包括氨基酸聚合物，所述聚合物已被天然地修饰或通过干预修饰；例如二硫键形成、糖基化、脂质化、乙酰化、磷酸化或任何其它操作或修饰，例如与标记或生物活性组分的缀合。
“分离的”生物组分(例如核酸或蛋白质或细胞器)是指这样的组分，所述组分已大体上与所述组分天然存在于其中的生物的细胞中的其它生物组分例如其它染色体和染色体外DNA和RNA、蛋白质和细胞器分离，或从所述其它组分纯化出来。已被“分离的”核酸和蛋白质包括通过标准纯化方法纯化的核酸和蛋白质。术语还涵盖通过重组技术以及化学合成制备的核酸和蛋白质。
如本文所用，术语“纯化的”不需要绝对纯度；相反地，它意欲作为相对术语。因此，例如，纯化的多肽制剂是其中多肽比所述多肽在其天然环境中更加富集的制剂。即将多肽与细胞组分分离。所谓“大体上纯化的”，其意指使得多肽代表几个至少60％，至少70％，至少80％，至少90％，至少95％，或至少98％或更多的细胞组分或材料已被除去的实施方案。同样地，该多肽可以是部分纯化的。“部分纯化的”意欲指少于60％的细胞组或材料被去除。这同样适用于多核苷酸。本文所公开的多肽可以通过本领域公知的任何方法进行纯化。
如上所述，抗原性多肽或其片段或变体是BTV或AHSV抗原性多肽。公开的多核苷酸和由其编码的多肽的片段和变体也包括在本发明中。“片段”意欲是多核苷酸的一部分或由其编码的抗原性氨基酸序列的一部分。多核苷酸的片段可以编码蛋白片段，所述片段保留天然蛋白的生物活性，从而具有如本文中其它地方指出的免疫原性活性。多肽序列的片段保留诱导动物的保护性免疫反应的能力。
“变体”意指大体上相似的序列。对于多核苷酸，变体包含缺失和/或添加一个或多个核苷酸在天然多核苷酸内的一个或多个位点上的缺失和/或添加，和/或一个或多个核苷酸在天然多核苷酸内的一个或多个位点上的置换。如本文使用的，“天然”多核苷酸或多肽分别包含天然存在的核苷酸序列或氨基酸序列。本发明的特定多核苷酸(即，参照多核苷酸)的变体还可通过由变体多核苷酸编码的多肽与由参照多核苷酸编码的多肽之间的序列同一性百分比的比较来评价。“变体”蛋白意指通过天然蛋白质内一个或多个位点上的一个或多个氨基酸的缺失或添加或天然蛋白质内一个或多个位点上的一个或多个氨基酸的置换，从天然蛋白质衍生的蛋白质。由本发明包括的变体蛋白具有生物学活性，即它们引发免疫反应的能力。
在一个实施方案中，本发明提供了包含一种或多种重组BTV或AHSV载体的组合物或疫苗，所述载体包含编码BTV或AHSV的至少一种多肽的一个或多个异源多核苷酸。所述多肽可以是选自VP1、VP2、VP3、VP4、VP6、VP5、VP7、NS1、NS2、NS3/3A的任何BTV或AHSV多肽。在一方面，重组BTV或AHSV载体包含来源于BTV或AHSV血清型1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25和26的基因组的载体主链。在另一个方面，异源多核苷酸编码来自BTV或AHSV的血清型的抗原，所述BTV或AHSV血清型与用作载体主链的BTV或AHSV的血清型不同。
在另一个实施方案中，重组BTV或AHSV载体包含编码多肽VP1、VP2、VP3、VP4、VP5、VP7、NS1、NS2、VP6和NS3/3A的异源多核苷酸，其中所述多肽可以是来自BTV或AHSV的不同血清型，例如血清型1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25和26。来自BTV或AHSV的不同血清型的多肽的不同组合可在下列表中进行举例说明。用于本发明的重组重配BTV(或AHSV)载体的命名法被定义为：名称的大写部分是指病毒的主链，随后是包括置换的蛋白质的下标，在此之前是区段的血清型来源，例如BTV-18VP1是指具有包含BTV-8的VP1基因的BTV-1主链的病毒。
表1包含来自一个BTV血清型的1或2种多肽和来自另一个BTV血清型的9或8种多肽的重配BTV的一些实例
VP1 VP2 VP3 VP4 VP5 VP6 VP7 NS1 NS2 NS3/3A
BTV-1 1 1 1 1 1 1 1 1 1 1
BTV-1<sub>8VP1VP2</sub> 8 1 1 1 1 1 1 1 1 1
BTV-1<sub>8VP2</sub> 1 8 1 1 1 1 1 1 1 1
BTV-1<sub>8VP3</sub> 1 1 8 1 1 1 1 1 1 1
BTV-1<sub>8VP4</sub> 1 1 1 8 1 1 1 1 1 1
BTV-1<sub>8VP5</sub> 1 1 1 1 8 1 1 1 1 1
BTV-1<sub>8VP6</sub> 1 1 1 1 1 8 1 1 1 1
BTV-1<sub>8VP7</sub> 1 1 1 1 1 1 8 1 1 1
BTV-1<sub>8NS1</sub> 1 1 1 1 1 1 1 8 1 1
BTV-1<sub>8NS2</sub> 1 1 1 1 1 1 1 1 8 1
BTV-1<sub>8NS3/3A</sub> 1 1 1 1 1 1 1 1 1 8
BTV-8 8 8 8 8 8 8 8 8 8 8
BTV-8<sub>1VP1</sub> 1 8 8 8 8 8 8 8 8 8
BTV-8<sub>1VP2</sub> 8 1 8 8 8 8 8 8 8 8
BTV-8<sub>1VP3</sub> 8 8 1 8 8 8 8 8 8 8
BTV-8<sub>1VP4</sub> 8 8 8 1 8 8 8 8 8 8
BTV-8<sub>1VP5</sub> 8 8 8 8 1 8 8 8 8 8
BTV-8<sub>1VP6</sub> 8 8 8 8 8 1 8 8 8 8
BTV-8<sub>1VP7</sub> 8 8 8 8 8 8 1 8 8 8
BTV-8<sub>1NS1</sub> 8 8 8 8 8 8 8 1 8 8
BTV-8<sub>1NS2</sub> 8 8 8 8 8 8 8 8 1 8
BTV-8<sub>1NS3/3A</sub> 8 8 8 8 8 8 8 8 8 1
BTV-1<sub>8VP1,VP2</sub> 8 8 1 1 1 1 1 1 1 1
BTV-1<sub>8VP2,VP3</sub> 1 8 8 1 1 1 1 1 1 1
BTV-1<sub>8VP2,VP5</sub> 1 8 1 1 8 1 1 1 1 1
BTV-8<sub>1VP1,VP2</sub> 1 1 8 8 8 8 8 8 8 8
BTV-8<sub>1VP2,VP3</sub> 8 1 1 8 8 8 8 8 8 8
BTV-8<sub>1VP2,VP5</sub> 8 1 8 8 1 8 8 8 8 8
本发明包括包含重配BTV的重组BTV载体，所述重配BTV具有式BTV-ABVPa、BTV-ABVPc,VPd、BTV-ABNSe、BTV-ABVPc,NSe，其中A和B＝1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25和26(对于BTV或AHSV血清型)；c和d＝1、2、3、4、5、6和7(对于VP1、VP2、VP3、VP4、VP5、VP6或VP7)；e＝1、2和3/3A(对于NS1、NS2或NS3/3A)。
在另一个方面，本发明提供了具有示于SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189或190的序列的BTV多肽及其变体或片段。
此外，来自绵羊类动物、牛类动物、山羊类动物或马类动物的BTV或AHSV多肽的同源物旨在本发明的范围之内。如本文所用，术语“同源物”包括直向同源物(orthologs)、类似物和种内同源物(paralogs)。术语“类似物”是指具有相同或类似功能，但已经在不相关的生物分别进化的两种多核苷酸或多肽。术语“直向同源物”是指来自不同物种，但已通过物种形成从一个共同祖先基因演化而来的两个多核苷酸或多肽。通常地，直向同源物编码具有相同或相似功能的多肽。术语“种内同源物”是通过基因组内的复制而相关的两种多核苷酸或多肽。种内同源物通常具有不同的功能，但这些功能可以相关。野生型BTV或AHSV多肽的类似物、直向同源物和种内同源物可与野生型BTV多肽相异在于翻译后修饰、氨基酸序列差异或两者。具体地，本发明的同源物将通常显示与野生型BTV或AHSV或多核苷酸序列的全部或部分至少80-85％，85-90％、90-95％、或95％、96％、97％、98％、99％的序列同一性，并且将显示相似的功能。变体包括等位基因变体。术语“等位基因变体”是指包含导致蛋白质的氨基酸序列的变化并且存在于天然群体(例如，病毒种或变种)中的多态性的多核苷酸或多肽。这样的天然等位基因变异通常可导致1-5％的多核苷酸或多肽的变异。等位基因变体可以通过对许多不同物种中的目标核酸序列进行测序来鉴定，所述测序可通过使用杂交探针来鉴定这些物种的相同基因的基因座来容易地进进。作为天然等位基因变异的结果并且不改变目标基因的功能活性的任何和所有这样的核酸变异和所得的氨基酸多态性或变异意在本发明的范围之内。
如本文中所用，术语“衍生物”或“变体”是指这样的多肽或编码多肽的核酸，其具有一个或多个保守氨基酸变异或其它小的修饰，以便(1)当与野生型多肽比较时，相应多肽具有基本上等同功能，或(2)针对所述多肽的抗体与野生型多肽发生免疫反应。这些变体或衍生物包括具有BTV或AHSV一级氨基酸序列的少量修饰的多肽，所述修饰可导致相较于未修饰对应多肽具有大体上相当的活性的肽。这样的修饰可以是有意的，如通过定点诱变，或可以是自发的。术语“变体”还涉及对序列的缺失、添加和置换，只要多肽发挥产生如本文中所定义的免疫反应的功能。
术语“保守变异”是另一种生物学上相似的残基对氨基酸残基的取代，或核酸序列的核苷酸的取代，所述取代使得编码的氨基酸残基不发生变化或为另一种生物学上相似的残基。在这方面，特别优选的置换通常是性质上保守性的，如上文中所述的。
本公开内容的多核苷酸包括作为遗传密码(例如用于特定宿主的最优化的密码子选择)的结果而为简并的序列。如本文中所用，“最优化的”是指经基因工程改造以增加其在给定的物种中的表达的多核苷酸。为了提供最优化的编码BTV或AHSV多肽的多核苷酸，可修饰BTV或AHSV蛋白基因的DNA序列以1)包含由特定物种中的高度表达的基因优先选择的密码子；2)在核苷酸碱基组成上包含A+T或G+C的含量至与在所述物种中发现的含量大体上相同的含量；3)形成所述物种的起始序列；或4)消除造成RNA的不稳定、不适当的多腺苷酸化、降解和终止，或形成二级结构发夹或RNA剪接位点的序列。BTV或AHSV蛋白在所述物种中的增加的表达可通过利用密码子选择在真核生物和原核生物或在特定物种中的分布频率来实现。术语“优先密码子选择的频率”是指由特定宿主细胞在使用核苷酸密码子指定给定的氨基酸上显示的偏好。存在20种天然氨基酸，其大部分由一个以上的密码子指定。因此，所有简并核苷酸序列包括在公开内容中，只要核苷酸序列编码的BTV或AHSV多肽的氨基酸序列在功能上不变。
可利用NCBI(美国国家生物技术信息中心)成对blast以及blosum62矩阵，使用标准参数来建立两个氨基酸序列之间的序列同一性(参见，例如，可在“国家生物技术中心信息”(NCBI，Bethesda,Md.,USA)服务器上，以及在Altschul等人中获得的BLAST或BLASTX算法；并且从而，该文献利用术语“blasts”提及使用算法或BLAST或BLASTX和BLOSUM62矩阵)。
关于序列的“同一性”可指具有相同核苷酸或氨基酸的位置的数目除以两个序列的更短序列中的核苷酸或氨基酸的数目，其中两个序列的比对可以根据Wilbur和Lipman算法(Wilbur和Lipman)，例如，使用20个核苷酸的窗口大小、4个核苷酸的字长和为4的缺口罚分(gap penalty)来测定，包括比对的序列数据的计算机辅助分析和解释可使用商购可得的程序(例如，IntelligeneticsTM Suite,Intelligenetics Inc.CA)来方便地进行。当RNA序列被认为是相似，或与DNA序列具有一定程度的序列同一性或同源性时，DNA序列中的胸苷(T)被认为等同于RNA序列中的尿嘧啶(U)。因此，RNA序列在本发明的范围内，并且可通过将DNA序列中的胸苷(T)认为等同于RNA序列中的尿嘧啶(U)来从DNA序列衍生。
两个氨基酸序列的序列同一性或序列相似性，或两个核苷酸序列之间的序列同一性可使用Vector NTI软件包(Invitrogen,1600 Faraday Ave.,Carlsbad,CA)来测定。
下列文献提供了用于比较序列的相对同一性或同源性的算法，并且相对前述内容另外地或可选择地，这些参考文献中的教导可用于测定百分比同一性或同源性：NeedlemanSB和Wunsch CD；Smith TF和Waterman MS；Smith TF,Waterman MS和Sadler JR；Feng DF和Dolittle RF；Higgins DG和Sharp PM；Thompson JD,Higgins DG和Gibson TJ；以及Devereux J,Haeberlie P和Smithies O。而且，无需过多实验，本领域普通技术人员可咨询用于测定百分比同源性的许多其它程序或参考文献。
可在不同“严格度”的条件下进行杂交反应。提高杂交反应的严格度的条件是公知的。参见例如，“Molecular Cloning:A Laboratory Manual”,第2版(Sambrook等人,1989)。
本发明还包括包含在载体分子或表达载体中以及可操作地连接于启动子元件和任选连接于增强子的BTV多核苷酸。
“载体”指重组DNA或RNA质粒或病毒，其包含待被体外或体内递送至靶细胞的异源多核苷酸。异源多核苷酸可以包含用于预防或治疗目的的目标序列，并且可任选地以表达盒的形式存在。如本文中所用，载体无须能够在最终的靶细胞或受试者中复制。该术语包括克隆载体和病毒载体。
术语“重组体”意指半合成或合成来源的多核苷酸，其不存在于自然界中或以在自然界中未发现的排列方式连接于另一个多核苷酸。
“异源”意指来源于在遗传上与其所被比较的实体(entity)的其余部分不同的实体。例如，多核苷酸可通过基因工程技术被置于来源于不同来源的质粒或载体中，并且为异源多核苷酸。从其天然编码序列取出并可操作地连接于除天然序列外的编码序列的启动子是异源启动子。
本发明涉及绵羊类动物，牛类动物，山羊类动物和猪类动物的疫苗或药物或免疫组合物，其可包括有效量的重组BTV抗原和药学上或兽医学上可接受的载体、赋形剂、佐剂或媒介物。
本文中描述的主题部分地针对与在植物或藻类表达系统中制备的BTV抗原相关的组合物和方法，所述BTV抗原具有高度免疫原性，并且保护动物免受来自同源和异源BTV菌株的攻击。
本发明涉及包含一种或多种重组BTV或AHSV载体和药学上或兽医学上可接受的载体、赋形剂、佐剂或媒介物的组合物或疫苗，所述载体包含编码BTV或AHSV的至少一种多肽的一个或多个异源多核苷酸。
在另一个实施方案中，药学上或兽医学上可接受的载体、赋形剂、佐剂或媒介物可以是油包水乳剂。在又一个实施方案中，油包水乳剂可以是水/油/水(W/O/W)三重乳剂。
本发明还包括包含在载体分子或表达载体中和任选地可操作地连接于启动子元件和任选地连接于增强子的BTV多核苷酸。
在另一个方面，本发明提供了与本发明的抗原性多肽，特别地与具有SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189或190中所示的序列的多肽具有至少70％、至少75％、至少80％、至少85％、至少90％、至少95％、96％、97％、98％或99％的序列同一性的多肽。
在另一个方面，本发明提供了上文中鉴定的BTV多肽(SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189或190)的片段和变体，其可由本领域普通技术人员使用公知的分子生物学技术来容易地制备。
变体是与SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189或190中所示的氨基酸序列具有至少75％、80％、85％、90％、95％、96％、97％、98％或99％的同一性的氨基酸序列的同源多肽。
在另一个方面，本发明提供了编码BTV多肽的多核苷酸，例如编码具有SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189或190中所示的序列的多肽的多核苷酸。在另一个方面，本发明提供了编码多肽或其保守变体、等位基因变体、同源物或免疫原性片段(包含这些多肽之一的至少8个或至少10个连续氨基酸)或这些多肽的组合的多核苷酸，所述多肽与具有SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189或190中所示的序列的多肽具有至少70％、至少75％、至少80％、至少85％、至少90％、至少95％、96％、97％、98％或99％的序列同一性。
在另一个方面，本发明提供了具有SEQ ID NO:63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131或132中所示的核苷酸序列的多核苷酸或其变体。在另一个方面，本发明提供了具有与SEQ ID NO:63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131或132中所示的序列的多核苷酸之一至少70％、至少75％、至少80％、至少85％、至少90％、至少95％、至少95％、96％、97％、98％或99％的序列同一性的多核苷酸或其变体。
本发明的多核苷酸可以包含另外的序列，例如在相同转录单位内的另外的编码序列，控制元件例如启动子、核糖体结合位点、5'UTR、3'UTR、转录终止子、多腺苷酸化位点、在相同或不同的启动子控制之下的另外的转录单位、允许克隆、表达、同源重组以及宿主细胞的转化的序列，并且可期望任何这样的构建体提供本发明的实施方案。
用于BTV或AHSV多肽、抗原、表位或免疫原表达的元件有利地存在于本发明的载体中。它以最低限度的方式包含起始密码子(ATG)、终止密码子和启动子以及任选地还有聚腺苷酸化序列(对某些载体如质粒和某些病毒载体，例如除痘病毒外的病毒载体)，或基本上由其组成或由其组成。当多核苷酸编码多蛋白质片段，例如BTV肽时，有利地在载体中将ATG置于阅读框的5'并将终止密码子置于3'。用于控制表达的其它元件可存在，例如增强子序列、稳定序列，例如内含子和允许蛋白质分泌的信号序列。
本发明还涉及包含载体，例如表达载体的制剂，例如治疗性组合物。制剂可包含一种或更多种载体，例如表达载体，例如体内表达载体，其包含和表达一种或多种BTV或AHSV多肽、抗原、表位或免疫原。在一个实施方案中，载体包含和表达多核苷酸，所述多核苷酸包含编码(且有利地表达)BTV或AHSV抗原、表位或免疫原的多核苷酸(在药学上或兽医学上可接受的载体、赋形剂或媒介物中)，基本上由其组成，或由其组成。因此，根据本发明的实施方案，制剂中的其它一种或多种载体包含(并且在适当的情况下所述载体表达)编码BTV多肽、抗原、表位或免疫原的一种或多种其它蛋白质或其片段的多核苷酸，基本上由所述多核苷酸组成或由所述多核苷酸组成。
根据另一个实施方案，制剂中的一种或多种载体包含编码BTV或AHSV多肽、抗原、表位或免疫原的一种或多种蛋白质或其片段的多核苷酸，基本上由所述多核苷酸组成，或由所述多核苷酸组成，所述一种或多种载体表达所述多核苷酸。在另一个实施方案中，制剂包含一种、两种或更多种载体，所述载体包含编码和表达(有利地在体内)BTV或AHSV多肽、抗原、融合蛋白或其表位的多核苷酸。本发明还涉及载体的混合物，所述载体包含编码并表达不同BTV或AHSV多肽、抗原、表位或免疫原，例如，来自不同动物物种(例如但不限于绵羊类动物、牛类动物、山羊类动物或猪类动物)的BTV或AHSV多肽、抗原、表位或免疫原的多核苷酸。
根据另一个实施方案，表达载体是质粒载体或DNA质粒载体，特别是体内表达载体。在一个具体的非限制性实例中，pVR1020或1012质粒((VICAL Inc.；Luke等人，1997；Hartikka等人，1996，参见例如，美国专利No.5,846,946和6,451,769)可以用作用于多核苷酸序列的插入的载体。pVR1020质粒来源于pVR1012并且包含人tPA信号序列。在一个实施方案中，人tPA信号在Genbank中于登录号HUMTPA14下包含氨基酸M(1)至氨基酸S(23)。在另一个具体的非限制性实例中，用作用于多核苷酸序列的插入的载体的质粒可在Genbank中于登录号U28070下包含马类动物IGF1的从氨基酸M(24)至氨基酸A(48)的信号肽序列。可被咨询或用于实践的关于DNA质粒的另外信息可见于例如美国专利No.6,852,705；6,818,628；6,586,412；6,576,243；6,558,674；6,464,984；6,451,770；6,376,473和6,221,362中。
术语质粒涵盖任何DNA转录单位，所述转录单位包含根据本发明的多核苷酸和其在期望的宿主或靶细胞中体内表达所必需的元件；且在这方面，应指出的是超螺旋或非超螺旋的环状质粒以及线性形式旨在本发明的范围内。
每一个质粒还包含或含有编码BTV或AHSV抗原、表位或免疫原的多核苷酸，其任选地与异源肽序列、变体、类似物或片段融合，可操作地连接于启动子或处于启动子的控制之下或依赖于启动子，或基本上由所述多核苷酸组成。一般而言，有利地使用在真核细胞中具有功能的强启动子。强启动子可以是但不限于人或鼠来源或任选具有另一个来源例如大鼠或豚鼠的即时早期巨细胞病毒启动子(CMV-IE)，超级启动子(Ni,M.等人，Plant J.7,661-676,1995.)。CMV-IE启动子可包含实际启动子部分，其可以与或可以不与增强剂部分相关联。可参考EP-A-260148，EP-A-323597，美国专利No.5,168,062、5,385,839和4,968,615，以及参考PCT申请No WO87/03905。CMV-IE启动子有利地是人CMV-IE(Boshart等人，1985)或鼠CMV-IE。
在更一般的术语中，启动子具有病毒、植物或细胞来源。除可成功地用于本发明的实践的CMV-IE外的强病毒启动子是SV40病毒的早期/晚期启动子或劳斯肉瘤病毒的LTR启动子。可有用地用于本发明的实施的强细胞启动子是细胞骨架基因的启动子，例如结蛋白启动子(Kwissa等人，2000)或肌动蛋白启动子(Miyazaki等人，1989)。
可使用组成型、可调控型或刺激依赖性启动子的任一种启动子。例如，组成型启动子可包括来自根癌土壤杆菌(Agrobacterium tumefaciens)的甘露碱合酶启动子。或者，可有利地使用热休克基因启动子、干旱诱导型基因启动子、病原体诱导型基因启动子、伤口诱导型基因启动子和光/暗诱导型基因启动子。可有用地使用受植物生长调节剂例如脱落酸、生长素、细胞分裂素和赤霉酸控制的启动子。还可选择提供组织特异性表达的启动子(例如，根、叶和花特异性启动子)。
质粒可包括其它表达控制元件。特别有利的是掺入稳定序列例如内含子序列，例如，玉米醇脱氢酶的内含子(Callis等人，Genes&Dev.1(10):1183-1200,Dec.1987)、hCMV-IE的第一内含子(PCT申请No.WO1989/01036)、兔β珠蛋白基因的内含子II(van Ooyen等人，1979)。在另一个实施方案中，质粒可含3'UTR。3'UTR可以是但不限于农杆菌胭脂碱合酶(agrobacterium nopaline synthase)(Nos)3'UTR(胭脂氨酸合酶：转录物作图和DNA序列。Depicker，A.等人J.Mol.Appl.Genet.,1982；Bevan,NAR,1984,12(22):8711-8721)。
关于除痘病毒外的质粒和病毒载体的聚腺苷酸化信号(polyA)，更多地使用牛生长激素(bGH)基因(参见U.S.5,122,458)的poly(A)信号，或兔β珠蛋白基因的poly(A)信号或SV40病毒的poly(A)信号。
“宿主细胞”或“细胞”是指已被遗传改变，或能够通过外源多核苷酸例如重组质粒或载体或ssRNA或dsRNA的施用遗传改变的原核或真核细胞。当指遗传上改变的细胞时，该术语是指最初改变的细胞及其后代。
在一个实施方案中，本文中公开的主题涉及产生重组BTV或AHSV载体的方法，其包括用a)编码BTV或AHSV的血清型的一种或多种多肽的RNA；和b)包含不同血清型的BTV或AHSV的完整基因组的转录物和包含编码a)中的抗原的转录物的缺失的RNA转染细胞。
在另一个实施方案中，本文中公开的主题涉及接种绵羊类动物、牛类动物、山羊类动物或猪类动物的方法，包括给绵羊类动物、牛类动物、山羊类动物或猪类动物施用有效量的疫苗，所述疫苗可包含有效量的重组BTV载体和药学上或兽医学上可接受的载体、赋形剂或媒介物。
在实施方案中，本文中公开的主题涉及引发免疫反应的方法，包括给绵羊类动物、牛类动物、山羊类动物或猪类动物施用包含绵羊类动物、牛类动物、山羊类动物，或猪类动物的重组BTV或AHSV载体的疫苗，其中免疫反应被引发。
施用可以是皮下或肌肉内施用。施用可以是无针的(例如Pigjet或Bioject)。
在本发明的一个实施方案中，可使用初免-加强(prime-boost)方案，该方案由使用至少一种共同的多肽、抗原、表位或免疫原的至少一次初次施用和至少一次加强施用组成。通常情况下，初次施用中使用的免疫组合物或疫苗在性质上与用作增压的那些免疫组合物或疫苗不同。然而，应指出的是，同样的组合物可用作初次施用和加强施用。该施用方案称为“初免-加强”。
根据本发明的初免-加强可包括用于表达编码抗原性多肽或其片段或变体的BTV编码序列或其片段的重组病毒载体。具体地，病毒载体可表达编码抗原性多肽的BTV或AHSV基因或其片段。本文中涉及的病毒载体包括但不限于痘病毒[例如，痘苗病毒或减毒痘苗病毒、禽痘病毒或减毒禽痘病毒(例如，金丝雀痘、鸡痘、dovepox、鸽痘、鹌鹑痘、ALVAC、TROVAC；参见例如，US 5,505,941、US 5,494,8070)、浣熊痘病毒、猪痘病毒等]、腺病毒(例如，人腺病毒、犬腺病毒)、疱疹病毒(例如，犬疱疹病毒、火鸡的疱疹病毒、马立克氏病病毒、传染性喉气管炎病毒、猫疱疹病毒、喉气管炎病毒(ILTV)、牛疱疹病毒、猪疱疹病毒)、杆状病毒、逆转录病毒等。在另一个实施方案中，禽痘表达载体可以是金丝雀痘载体例如ALVAC。在另一个实施方案中，禽痘表达载体可以是鸡痘载体例如TROVAC。待表达的本发明的BTV或AHSV抗原被插入在特定痘病毒启动子的控制下，所述启动子是除了其它以外，例如桑灯蛾昆虫痘病毒(Amsacta moorei)42K启动子(Barcena,Lorenzo等人，2000)、牛痘启动子7.5kDa的(Cochran等人，1985)、牛痘启动I3L(Riviere等人，1992)、牛痘启动子HA(Shida，1986)、牛痘启动子ATI(Funahashi等人，1988)、牛痘启动子H6(Taylor等人，1988b；Guo等人，1989；Perkus等人，1989)。
在另一个实施方案中，禽痘表达载体可以是金丝雀痘载体，如，ALVAC。BTV或AHSV多肽、抗原、表位或免疫原可以是BTV或AHSV VP2或BTV VP5。病毒载体可以是vCP2289，其编码BTV密码子最优化的合成VP2和VP5(参见US 2007/0280960)。
在本发明的初免-加强方案的另一个方面，施用包含本发明的重组BTV载体的组合物，随后施用包含BTV或AHSV抗原的疫苗或组合物、或灭活的病毒疫苗或包含或表达BTV或AHSV抗原的DNA质粒疫苗或组合物。同样地，初免-加强方案可包括施用包含灭活的病毒疫苗的疫苗或组合物，或包含BTV或AHSV抗原的组合物，或包含或表达BTV或AHSV抗原的DNA质粒疫苗或组合物，随后施用包含本发明的重组BTV或AHSV载体的组合物。还应进一步指出，初次和第二施用可包括包含本发明的BTV抗原的组合物。
初免-加强方案包括使用共同的多肽和/或其变体或片段的至少一次初次施用和至少一次加强施用。在初次-施用中使用的疫苗可在性质上与用作随后的加强疫苗的那些疫苗不同。初次施用可包括一次或多次施用。类似地，加强施用可包括一次或多次施用。
用于为哺乳动物的目标物种的组合物的剂量体积，例如基于病毒载体的绵羊类动物、牛类动物、山羊类动物或猪类动物的组合物，例如基于非痘病毒-病毒-载体的组合物的剂量体积通常为约0.1至约5.0ml、约0.1至约3.0ml和约0.5ml至约2.5ml。
可在最后一次免疫后约2至4周，通过用BTV的毒株例如BTV-1/2/3/4/8/9/16或17株攻击动物例如绵羊类动物、牛类动物、山羊类动物或猪类动物来测试疫苗的效力。例如，BTV株可以是血清型17，其最初是从来自Tulare County,CA的绵羊的血液分离而来的(参见Bonneau,DeMaula等人2002；DeMaula,Leutenegger等人2002)。BTV株也可以是血清型8，目前可从Merial Limited获得的灭活的疫苗。
其它菌株可以包括BTV1(澳大利亚分离株)、BTV1(南非分离株)、BTV2(美国分离株)、BTV3(南非分离株)、BTV4-9、BTV10(美国分离株)、BTV11(美国分离株)、BTV12、BTV13(美国分离株)、BTV14-17、BTV17(美国分离株)、BTV18、BTV19、BTV20(澳大利亚分离株)、BTV21-24或科西嘉岛BTV。
将同源和异源菌株用于攻击以测试疫苗的效力。动物可经真皮内、皮下、喷雾、鼻内、眼内、气管内和/或经口攻击。
对于BTV，评估牛类动物和山羊类动物的广泛的血管损伤。同样地对于BTV，评价绵羊类动物的口、鼻和前胃的粘膜的卡他性炎症、蹄的冠状带和蹄叶的炎症、上皮的擦伤、颊粘膜的坏死以及肿胀/发炎的/蓝舌头和嘴。可在攻击后从所有动物收集拭子以用进行病毒分离。存在或不存在，病毒抗原在上述组织中的存在或不存在可通过定量实时逆转录聚合酶链反应(qRRT-PCR)进行评价。可在攻击前和攻击后收集血液样品，可就抗BTV特异性抗体的存在分析所述样品。
初免-加强施用可有利地间隔2-6周进行，例如，间隔约3周。根据一个实施方案，还设想半年加强或一年加强(有利地使用基于病毒载体的疫苗)。在第一次施用时，动物是有利地为至少6至8周龄。
将包含用于初免-加强方案的本发明的重组抗原性多肽的组合物包含于药学上或兽医学上可接受的媒介物、稀释剂或赋形剂中。本发明的方案保护来自绵羊类动物、牛类动物、山羊类动物或猪类动物的动物免受BTV或AHSV的侵害和/或防止疾病在受感染的动物中进展。
各种施用优选间隔1-6周，更具体地间隔约3周进行。根据优选的模式，还设想优选使用疫苗的基于病毒载体的免疫组合物的每年加强。动物在第一次施用时优选为至少1日龄。
本领域普通技术人员应当理解，本文中的公开内容以举例说明的方式提供，并且本发明不限于此。根据本文中的公开内容和本领域的知识，本领域普通技术人员可确定施用次数、施用途径和用于每一个注射方案的剂量而无需任何过度实验。
本发明设想至少一次对动物施用有效量的根据本发明制造的治疗组合物。动物可以是雄性、雌性、怀孕雌性和新生儿。该施用可以通过各种途径，包括但不限于肌内(IM)、真皮内(ID)或皮下(SC)注射或通过鼻内或口服施用进行。根据本发明的治疗组合物还可通过无针装置(例如，例如利用Pigjet、Dermojet、Biojector、Avijet(Merial,GA,USA)、Vetjet或Vitajet装置(Bioject,Oregon,USA))施用。施用质粒组合物的另一种方法是使用电穿孔(参见，例如，Tollefsen等人,2002；Tollefsen等人，2003；Babiuk等人，2002；PCT申请No.WO99/01158)。在另一个实施方案中，通过基因枪或金颗粒轰击将治疗组合物递送给动物。
在一个实施方案中，本发明提供了治疗有效量的制剂的施用，所述制剂用于将BTV或AHSV抗原或表位递送至靶细胞并在所述靶细胞中表达。治疗有效量的确定对于本领域普通技术人员来说是常规实验。在一个实施方案中，制剂包含表达载体和药学上或兽医学上可接受的载体、媒介物或赋形剂，所述表达载体包含表达BTV或AHSV抗原或表位的多核苷酸。在另一个实施方案中，药学上或兽医学上可接受的载体、媒介物或赋形剂促进多核苷酸至宿主动物的转染或其它方式的转移和/或改善载体或蛋白质在宿主中的保存。
在一个实施方案中，本文中公开的主题提供了用于区分受感染的动物与接种的动物(DIVA)的检测方法。
目前，存在几种可用的BTV疫苗。Merial和Intervet都提供灭活的BTV8疫苗。最近已描述了区分BTV接种动物与BTV感染的动物的方法(Sílvia C.Barros等人,Veterinary-Microbiology,2009).
本文中公开了本发明的疫苗或组合物的使用允许检测动物的BTV或AHSV感染。本文中公开了本发明的疫苗或组合物的使用允许通过区分受感染的动物与接种的动物(DIVA)来检测动物的感染。本文中公开了使用基于BTV-NS3的免疫原性检测法，例如，NS3特异性ELISA诊断动物的BTV感染的方法。
制品
在实施方案中，本文中公开的主题涉及用于执行诱发或诱导免疫应答的方法的试剂盒，其可以包括任一种重组的BTV或AHSV免疫组合物或疫苗，或灭活的BTV或AHSV免疫组合物或疫苗，重组的BTV或AHSV病毒组合物或疫苗，和用于执行该方法的说明书。
本发明的另一个实施方案是用于进行诱导动物的针对BTV或AHSV的免疫或保护性应答的方法的试剂盒，其包括包含本发明的BTV抗原的组合物或疫苗和重组BTV或AHSV病毒免疫组合物或疫苗，以及用于进行以引发动物的免疫反应的有效量进行递送的方法的说明书。
本发明的另一个实施方案是用于进行诱导动物的针对BTV或AHSV的免疫或保护性应答的方法的试剂盒，其包括包含本发明的BTV或AHSV抗原的组合物或疫苗和灭活的BTV免疫组合物或疫苗，以及用于进行以引发动物的免疫反应的有效量进行递送的方法的说明书。
如上文中描述的，本发明的另一个方面涉及用于进行根据本发明的初免-加强接种的试剂盒。试剂盒可包含至少两个小瓶：包含用于根据本发明的初次接种的疫苗或组合物的第一小瓶，和包含用于根据本发明的加强接种的疫苗或组合物的第二小瓶。试剂盒可有利地包含用于另外的初次接种或另外的加强接种的另外的第一或第二小瓶。
本发明包括下列实施方案。在实施方案中，公开了包含重组BTV或AHSV载体和药学上或兽医学上可接受的载体、赋形剂、佐剂或媒介物的组合物。在实施方案中，公开了其中药学上或兽医学上可接受的载体、赋形剂、佐剂或媒介物是油包水乳剂或水包油乳剂的上述组合物。在另一个实施方案中，公开了接种对绵羊类动物、牛类动物、山羊类动物或猪类动物BTV或AHSV易感的动物的方法，所述方法包括给动物施用上述组合物。在实施方案中，公开了接种对绵羊类动物、牛类动物、山羊类动物或猪类动物BTV或AHSV易感的动物的方法，其包括初免-加强方案。在一个实施方案中，公开了诊断动物的流感感染的方法。在另一个实施方案中，公开了用于初免-加强接种的试剂盒，其包括至少两个小瓶，其中第一小瓶包含本发明的组合物，第二小瓶包含用于加强-接种的组合物，所述组合物包括包含重组病毒载体的组合物，或包含灭活的病毒组合物的组合物，或包含或表达BTV或AHSV抗原的DNA质粒组合物。
药学上或兽医学上可接受的载体或媒介物或佐剂或赋形剂对于本领域普通技术人员是公知的。例如，药学或兽医学上可接受的载体或媒介物或赋形剂可以是0.9％的NaCl(例如，盐水)溶液或磷酸盐缓冲液。可用于本发明的方法的其他药学或兽医学上可接受的载体或媒介物或赋形剂包括但不限于，聚(L-谷氨酸)或聚乙烯吡咯烷酮。药学上或兽医学上可接受的载体或媒介物或赋形剂可以是促进载体(或从本发明的载体体外表达的蛋白质)的施用的任何化合物或化合物的组合；有利地，载体、媒介物或赋形剂可促进转染和/或改善载体(或蛋白质)的保存。剂量和剂量体积在本文中于一般性描述中进行了讨论，并且还可由本领域普通技术人员根据与本领域的知识结合阅读的本公开内容来测定，而无需任何过度实验。
有利地但非唯一地适合于质粒的包含季铵盐的阳离子脂质有利地是具有下列通式的脂质：
其中R1为具有12至18个碳原子的饱和或不饱和的直链脂烃基，R2为含有2或3个碳原子的另一个脂烃基，并且X是胺基或羟基，如DMRIE。在另一个实施方案中，还可将阳离子脂质与中性脂质例如DOPE结合。
在这些阳离子脂质中，优选考虑DMRIE(N-(2-羟乙基)-N,N-二甲基-2,3-双(十四烷氧基)-1-丙烷铵；WO96/34109)，有利地与中性脂质，有利地DOPE(二油酰-磷脂酰-乙醇胺；Behr，1994)结合以形成DMRIE-DOPE。
有利地，临时和有利地与制剂施用的同时或在制剂施用前不久形成质粒与佐剂的混合物；例如，在施用前不久或即将施用时，有利地形成质粒-佐剂混合物，以便在施用前得到足够的时间以便所述混合物形成复合物，例如在施用前约10至约60分钟，例如施用前约30分钟。
当DOPE存在时，DMRIE:DOPE的摩尔比有利地为约95:约5至约5:约95，更有利地约1:约1，例如1:1。
DMRIE或DMRIE-DOPE佐剂:质粒的重量比可以为约50:约1至约1:约10，例如约10:约1至约1:约5，约1:1至约1:约2，例如，1:1至1:2。
在另一个实施方案中，药学上或兽医学上可接受的载体、赋形剂、媒介物或佐剂可以是油包水乳剂。适合的油包水乳剂的实例包括油基油包水乳剂疫苗，其在4℃是稳定的并且具有流动性，其包含：6至50v/v％，优选12至25v/v％的含抗原水相，50至94v/v％的全部或部分包含下述油的油相：不可代谢的油(例如，矿物油例如石蜡油)和/或可代谢的油(例如，植物油，或脂肪酸、多元醇或醇酯)、0.2至20p/v％，优选3至8p/v％的表面活性剂，后者是全部或部分的，或存在于混合物(聚甘油酯，所述聚甘油酯优选是聚甘油(聚)蓖麻油酸酯，或聚氧乙烯蓖麻油或其他氢化聚氧乙烯蓖麻油)。可用于油包水乳剂的表面活性剂的实例包括乙氧基化脱水山梨醇酯(例如，聚氧乙烯(20)脱水山梨糖醇单油酸酯可获自AppliChem，Inc.,Cheshire,CT)和脱水山梨醇酯(例如，脱水山梨糖醇单油酸酯 可购自Sigma Aldrich,St.Louis,MO)。此外，关于油包水乳剂，也参见美国专利No.6,919,084，例如，其实施例8，其通过引用并入本文。在一些实施方案中，含有抗原的水相包含含有一种或多种缓冲剂的盐水溶液。适合的缓冲液的实例是磷酸盐缓冲盐水。在有利的实施方案中，油包水乳剂可以是水/油/水(W/O/W)三重乳剂(美国专利No.6358500)。其它适合的乳剂的实例描述于美国专利No.7,371,395。
根据本发明的免疫组合物和疫苗可包含一种或多种药学上或兽医学上可接受的载体、赋形剂、媒介物或佐剂或基本上由其组成。用于本发明的实施的适合的载体或佐剂是(1)丙烯酸或甲基丙烯酸的聚合物、马来酸酐和烯基衍生聚合物，(2)免疫刺激序列(ISS)，例如具有一个或多个非甲基化CpG单位的寡脱氧核糖核苷酸序列(Klinman等人，1996；WO98/16247)，(3)水包油乳剂，例如由M.Powell,M.Newman,Plenum Press 1995出版的“Vaccine Design,The Subunit and Adjuvant Approach”的第147页上描述的SPT乳剂和相同著作的第183页上描述的乳剂MF59，(4)含有季铵盐的阳离子脂质，例如DDA，(5)细胞因子，(6)氢氧化铝或磷酸铝，(7)皂苷或(8)在通过引用并入本申请的任何文献中讨论的其他佐剂，或(9)其任意组合或混合物。
特别适合于病毒载体的水包油乳剂(3)可以基于：轻液体石蜡油(欧洲药典类型)，类异戊二烯油例如角鲨烷，角鲨烯，因烯烃例如异丁烯或癸烯的低聚化而产生的油，具有直链烷基的酸或醇的酯，例如植物油、油酸乙酯、丙二醇、二(辛酸酯/癸酸酯)、甘油三(辛酸酯/癸酸酯)和丙二醇二油酸酯，或支链脂肪醇或酸的酯，特别是异硬脂酸酯。
将油与乳化剂组合使用以形成乳剂。乳化剂可以是非离子型表面活性剂，例如：一方面脱水山梨糖醇、二缩甘露醇(例如无水油酸甘露醇酯)、甘油、聚甘油或丙二醇的酯与另一方面油酸、异硬脂酸、蓖麻油酸或羟硬脂酸的酯，所述酯任选地被乙氧基化，或为聚氧丙烯-聚氧乙烯共聚物嵌段，例如Pluronic，例如L121。
在类型(1)佐剂聚合物中，优选考虑交联的丙烯酸或甲基丙烯酸的聚合物，特别地通过糖或多元醇的聚烯基醚交联的聚合物。这些化合物在卡波姆的名义下是已知的(Pharmeuropa,第8卷,no.2,1996年6月)。本领域普通技术人员还可以参考美国专利No.2,909,462，其提供通过多羟基化合物交联的这样的丙烯酸类聚合物，所述多羟基化合物具有至少3个羟基，优选不超过8个这样的基团，至少三个羟基的氢原子被具有至少2个碳原子的不饱和脂烃基替换。优选基团是那些包含2至4个碳原子的基，例如乙烯基、烯丙基和其它烯属不饱和基团。所述不饱和基团还可含有其它取代基，例如甲基。在卡波姆名义出售的产品(BF Goodrich,Ohio,USA)是特别适合的。它们通过烯丙基蔗糖或烯丙基季戊四醇交联。其中，提及卡波普974P、934P和971P。
关于马来酸酐-烯基衍生共聚物，优选考虑EMA(Monsanto)，其是直链或交联的乙烯-马来酸酐共聚物，并且它们例如通过二乙烯基醚交联。也可参考J.Fields等人,1960。
关于结构，丙烯酸或甲基丙烯酸聚合物和EMA优选由具有下列通式的基本单元形成：
其中：
-R1和R2可以相同或不同地代表H或CH3
-x＝0或1，优选地x＝1
-y＝1或2，x+y＝2。
对于EMA，x＝0并且y＝2，以及对于卡波姆x＝y＝1。
这些聚合物可溶于水或生理盐水溶液(20g/l NaCl)，可以例如利用苏打(NaOH)将pH值调整至7.3至7.4，以提供其中可掺入表达载体的佐剂溶液。最终免疫或疫苗组合物中的聚合物浓度可在约0.01至约1.5％w/v、约0.05至约1％w/v和约0.1至约0.4％w/v的范围内。
细胞因子(5)可以以蛋白质形式存在于免疫或疫苗组合物中，或可与其免疫原或表位一起在宿主中共表达。优先考虑通过与表达其免疫原或表位的载体相同的载体或通过其单独的载体进行的一种或多种细胞因子的共表达。
本发明包括制备这样的组合组合物；例如通过，有利地与佐剂、载体、细胞因子和/或稀释剂一起，混合活性成分。
可用于本发明的细胞因子包括但不限于粒细胞集落刺激因子(G-CSF)、粒细胞/巨噬细胞集落刺激因子(GM-CSF)、干扰素α(IFNα)、干扰素β(IFNβ)、干扰素γ、(IFNγ)、白细胞介素-1α(IL-1α)、白细胞介素-1β(IL-1β)、白细胞介素-2(IL-2)、白细胞介素-3(IL-3)、白细胞介素-4(IL-4)、白细胞介素-5(IL-5)、白细胞介素-6(IL-6)、白细胞介素-7(IL-7)、白细胞介素-8(IL-8)、白细胞介素-9(IL-9)、白细胞介素-10(IL-10)、白细胞介素-11(IL-11)、白细胞介素-12(IL-12)、肿瘤坏死因子α(TNFα)、肿瘤坏死因子β(TNFβ)和转化生长因子β(TGFβ)。应理解，可将细胞因子与本发明的免疫或疫苗组合物共同施用和/或依次施用。因此，例如，本发明的疫苗还可包含外源核酸分子，所述外源核酸分子在体内表达合适的细胞因子，例如与待接种的或将在其中引发免疫反应的该宿主匹配的细胞因子(例如，用于待给牛类动物施用的制剂的牛细胞因子)。
有利地，根据本发明的免疫组合物和/或疫苗包含引发治疗反应的有效量的一种或多种本文中论述的多肽，或基本上由所述有效量的所述多肽组成或由所述有效量的所述多肽组成；并且有效量可根据本公开内容，包括并入本文的文献以及本领域的知识来确定，而无需过度实验。
在基于所述表达的多肽的免疫组合物和/或疫苗的情况下，剂量可包括约1μg至约2000μg，有利地约50μg至约1000μg，和更有利地约100μg至约500μg的BTV或FMDV抗原、表位或免疫原。剂量体积可以为约0.1至约10ml，有利地约0.2与约5ml。
本发明现在将通过以下非限制性实施例来进一步描述。
【具体实施方式】
实施例
使用由J.Sambrook等人(Molecular Cloning:A Laboratory Manual，第2版，ColdSpring Harbor Laboratory,Cold Spring Harbor,New York,1989)描述的标准生物学技术进行DNA插入物(insert)、质粒和重组病毒或植物载体的构建。
实施例1重配BTV的产生
细胞和病毒
BSR细胞是BHK-21细胞的克隆，并被维持在补充有5％胎牛血清(FBS)和25μg/ml青霉素/链霉素(p/s,Gibco)的Dulbecco改良伊格尔培养基(DMEM,Gibco)中。将Vero细胞维持在补充有5％FBS和p/s的DMEM培养基中。CPT-Tert细胞是绵羊脉络丛细胞的永生化品系(59)，并且被维持在补充有10％FBS和p/s的Iscove改良Dulbecco培养基(IMDM,Gibco)中。将所有哺乳动物细胞在具有5％CO2的润湿培养箱中于37℃进行温育。
BTV-1是南非血清型1参考菌株，并已用作第一BTV反向遗传学系统的基础。欧洲BTV-8株(BTV-8NET2008/06(IAH环状病毒集合))是在荷兰从临床上受影响的牛分离的，并且具有仅在KC细胞中传代一次，随后在BHK-21细胞中再进行一次传代的传代史。
质粒
先前已描述了用于BTV-1和BTV-8的拯救的质粒(Ratinier等人，2011)。每个质粒包含单个BTV cDNA拷贝的侧翼连接5'T7启动子和3'限制位点的基因组区段。这样排列T7启动子和限制性位点，以便用适当的限制性内切酶线性化的质粒允许体外转录具有真实的BTV末端的加帽RNA转录物。商业(GenScript)合成在与其它拯救质粒相同的背景中包含BTV-2、BTV-4和BTV-9(13)的野外强毒分离株(virulent field isolates)的Seg-2的另外质粒。
反向遗传学
用合适的限制性酶线性化包含BTV-1或BTV-8或所得的突变体的基因组区段的质粒，随后用苯酚-氯仿提取进行纯化。按照制造商的说明书，使用mMESSAGE mMACHINET7Ultra试剂盒(Ambion)将消化的质粒用作用于体外转录的模板。按照制造商的方案，通过苯酚/氯仿提取和通过illustra Microspin G25柱(GE Healthcare Life Sciences)依次纯化ssRNA。
按照期望的重配株装配单个RNA的组合(参见表1和图1)。
在转染前24h，将2×105个BSR细胞涂板在无抗生素的生长培养基中的12孔板中。使用Lipofectamine2000，利用RNA转染细胞2次。对于第一转染，将1×1011个拷贝的体外转录的编码VP1、3、4、6、NS1和NS2的BTV样加帽的RNA与含有RNAsin Plus(0.5U/μl,Promega)的OptiMEM(Gibco)以10μl的终体积混合。将2μlLiptofectamine2000与10μl的OptiMEM(含有RNAsin Plus)混合，在与RNA组合之前温育5分钟。在将转染复合物逐滴添加至细胞之前，使转染复合物形成20分钟。第一转染后约18小时，替换培养基，并按照第一转染第二次转染细胞，但用全部10个区段和3μl脂质体。第二次转染后4小时，将培养基替换为琼脂覆盖。
在整个本报告中采用标准命名法：名称的大写部分是指病毒的主链，随后是包括取代的蛋白质的下标，下标之前是区段的血清型来源，例如BTV-18VP1是指具有BTV-1主链，包含BTV-8的VP1基因的病毒。
描述重配BTV的产生的示意图示于图6中。
生长曲线
关于病毒生长的体外分析，在感染前一天，将2×105个CPT-Tert细胞/孔或3×106个KC细胞/孔涂板在1ml生长培养基中的12孔板中。将细胞在含有相关病毒的培养基中在37℃温育2小时。随后弃去培养基，用DMEM洗涤细胞一次，随后用1ml的适当的新鲜生长培养基进行温育。在感染后(p.i.)0、8、24、48和72小时取出100μl的上清液样品，并用100μl的新鲜生长培养基替换。通过在500×g离心5分钟澄清100μl样品，然后于4℃贮存。随后通过在BSR细胞中进行有限稀释测定法(60)滴定样品，病毒滴度表示为TCID50/ml。
噬菌斑测定
在用BTV感染之前24h，将CPT-Tert细胞以4×105个细胞/孔的密度接种在6孔板中。细胞在37℃感染2小时。之后，将含病毒培养基弃去，用DMEM洗涤细胞，在3ml的半固体覆盖层(1.2％的补充有4％的FBS和p/s的DMEM中的Avicel)中温育72h。随后弃去覆盖层，用PBS洗涤洗涤细胞，随后用甲醛中的结晶紫染色持续>1h。
中和测定
使用50μl的在含有3％FBS的DMEM中稀释的各测试血清在96孔板中一式四份地制备BTV-1或BTV-8特异性抗血清的三倍连续稀释液。随后将100TCID50的BTV-1、BTV-8和选择的重配株添加至相应的孔和平板中，并在37℃温育1h。最后，2.5×104个Vero细胞添加至各孔中，将板在具有5％CO的湿润的培养箱中于37℃温育5天，随后目视评估病毒CPE。随后使用Reed-Muench法(60)来测定定义为血清稀释抑制50％的Vero细胞培养物的BTV感染的血清稀释度的每一个血清样品的保护剂量50(PD50)。将悬浮BHK-21细胞按比例放大用于重配BTV的大规模生产。将收获的重配BTV灭活。将适合的佐剂加至重配BTV以用于疫苗配制。
结果
反向遗传学被用来确定是否有可能将BTV-1或BTV-8的任何个别区段重配成替代主链。在BTV-1的主链中具有BTV-8的单个区段的病毒的所有组合被成功拯救，反之亦然(图1)。通过来自每一个区段的片段的RT-PCR扩增和测序检查每个重配病毒的基因型(图1)。结果表明，任何BTV区段可在BTV-1与BTV-8之间重配。
使用噬菌斑测定并通过进行病毒的生长曲线来评估每一个重组病毒的体外生长特性。从两个独立轮次的噬菌斑测定来测定平均噬菌斑的直径。在大多数情况下，噬菌斑直径基本上不受影响，并与替代的个别区段被插入其中的亲代主链几乎无差异(图2A-B)。然而，一些病毒始终显示相对于亲代主链的较小或较大的噬菌斑表型。BTV-8Seg-8(编码NS2)至BTV-1主链的替代(BTV-18NS2)导致比野生型BTV-1小约33％的噬菌斑(图2A)。相对的重配株即BTV-81NS2的噬菌斑，也减少，尽管程度更低(图2B)。始终产生具有相较于亲代病毒更小的直径的噬菌斑的其他单重配株是具有编码VP1、VP4和NS1的重配区段的重配株。
进行生长曲线分析以评估每个重配株的生长动力学。多数重配株表现与相应野生型病毒(图3)相似的生长动力学(图3)。
若干研究表明，VP2与BTV血清型有着千丝万缕的联系。然而，也有人认为VP5有助于界定特定菌株的血清型。为了调查VP2和VP5对血清型确定的相对贡献，使用重配株BTV-1、BTV-8和具有BTV-1或BTV-8和单独的异源VP2或VP2和VP5的主链的重配株(BTV-18VP2、BTV-18VP2,VP5、BTV-81VP2和BTV-81VP2,VP5)进行血清中和测定。BTV-1和BTV-8中和抗血清来自于接种抗BTV-1或BTV-8疫苗并用同源株攻击的动物。
结果显示，BTV-8抗血清完全中和野生型BTV-8，同时显示了对BTV-1无中和能力(图4A)。类似地，通过BTV-1抗血清但非BTV-8抗血清中和BTV-1(图4A)。
利用包含异源病毒的单独的VP2或VP2和VP5的重配株的实验显示当血清匹配VP2蛋白时，病毒才被中和，从而确认研究使血清型唯一地与VP2相关联。
表明单独的VP2可决定血清型的结果促使我们产生并入来自血清型的选择的Seg-2的另外的BTV-1重配株。分别导致BTV-12VP2、BTV-14VP2和BTV-19VP2的包含BTV-2、BTV-4和BTV-9的VP2的BTV-1重配株病毒被成功地拯救。在所有情况下，与野生型病毒等同地生长的病毒被拯救(图5)。
实施例2接种的动物的临床和血清学研究
将使用BTV-1主链和用(VP2BTV8)或(VP2BTV8+VP5BTV8)替换(VP2BTV1)或(VP2BTV1+VP5BTV1)的两个构建体分别用于攻击研究。被产生来配制测试疫苗的抗原的特性概述于下表2中。
表2抗原的特征
抗原 主链 蛋白质替代(和血清型)
VP2 BTV-1 VP2(BTV-8)
VP2+VP5 BTV-1 VP2(BTV-8)+VP5(BTV-8)
生长重配病毒，将其灭活和处理，以产生两个抗原批次。通过将这些抗原与作为佐剂的铝凝胶(2.7mg氢氧化铝)和皂苷(30HU皂苷/1mL疫苗剂量)混合来将这些抗原配制成疫苗。测试的疫苗列于表3。
表3疫苗的特征
*:通过在AI(Active Ingredient)上进行定量斑点印迹测量的VP2BTV8含量
**:相同mL的非浓缩AI
将疫苗在1或2次注射中施用至常规BTV血清阴性绵羊。保护是通过在接种完成后21天进行强毒BTV-8攻击并且随后的临床和病毒学监测来评估保护作用。按照下表4进行绵羊的攻击研究。
表4
在接种期之前，监测所有动物的健康状况和直肠温度。在D0和/或D21，来自组G1至G6的每一个动物接受1个剂量的1mL适当的疫苗，如表4中描述的。在注射部位局部消毒后，在胸的左(D0)或右(D21)侧面、肘部旁边通过皮下途径进行注射。来自组G7的动物保持不处理并用作对照。
对于所有动物，在D42(挑战前)记录直肠温度，随后从D47至D56每天记录直肠温度。从D47至D56，记录所有动物的一般行为和身体状况。对于一般条件：良好被赋予0分；冷淡被赋予1分；抑郁被赋予2分；屈服被赋予3分。对于身体状况：正常被赋予0分；瘦被赋予1分；恶病质被赋予2分。
从D47至D56，记录在蓝舌病感染期间观察到的临床体征，包括：
-充血和/或水肿，尤其是头部：眼、鼻孔、耳、嘴唇、倒角(chamfer)、下颌间空间
-多涎
-鼻涕/痂皮
-哀怨咩咩
-瘀点
-运动烦恼(跛行)
-呼吸困难(咳嗽/呼吸困难)
-消化麻烦(腹泻)
-红斑
当存在时记录其他临床体征。在其中未进行个体临床监测的日子，每日检查群体的一般健康状况，作为每日护理和维持的部分。
在D0(接种前)、D21(接种疫苗前)、D42(攻击前)和D56，利用光管通过颈内穿刺从所有绵羊采样血液样品。处理所有样品以收集血清。在将它们转移至临床分析实验室之前，将血清等分和热灭活(56℃，30分钟)。通过血清中和测试在所有采样日期测定个体血清的BTV-8特异性抗体滴度。
血清中和试验，按照目前临床分析实验室使用技术(N°002488)进行血清中和测度。简言之，在微量滴定板中以始于1/3的3倍稀释度(0.48Log10)测试血清。将100微升的稀释血清与50微升的给定的BTV血清型(血清型8)的病毒悬浮液一起在37℃温育1小时。随后将50微升的每ml含500000个细胞的VERO细胞悬浮液添加至混合物中，将板在37℃进行温育。在7天的温育后，板的读取基于致细胞病变效应。以Log10(PD50％)表示的血清滴度在角度变换后通过回归来计算。
在D42(攻击前)、D47、D49、D51、D54和D56，利用EDTA管通过颈内穿刺对所有绵羊采集血液样品。
为了检测和定量血液中的蓝舌病病毒RNA，通过qRT-PCR测试对这些样品进行分析。按照当前由临床分析实验室使用的技术No.200336(自动化方法)进行这些测定。
简言之，在使用商业试剂盒(Nucleospin multi 96)提取后，首先通过热处理使RNA变性。随后使用来自Invitrogen Super Script IIIPlatinium One step试剂盒的MGB探针、特异性引物和试剂温育一个等分部分，以允许在热循环仪上进行扩增。以一式两份进行该步骤。荧光信号与合成的DNA的量成正比。扩增涉及24种BTV血清型的任何一种。将样品中的BTV核酸的量与标准化RNA样品相比较。RNA的量表示为Log10RNA拷贝数/mL血液，考虑到在每一个一式两份中获得结果(当二者都是阳性时)，和当发现一式两份之一是阴性的，仅有一个阳性)。技术的阳性截断点95％(定义为可在95％的测试运行被检测的每体积样品的目标序列的最少数量)或阳性响应阈值95％(PRT95)经测定为3.68 log10 RNA拷贝/mL。低于3.68 log10 RNA拷贝/mL的样品滴定的结果表示为<PRT95，并且被认为是阴性的。同样地，给出≥P RT95的样品滴定的结果，该结果被认为是阳性的。
为了进行结果描述(平均值、标准差和曲线下面积的计算)和分析，将3.68 log10RNA拷贝/mL的滴度归因于阴性样品。
过热结果
攻击后平均温度的演化示于图8中。极大过热的分散示于图9。
始于D48(即攻击后6天)在对照组中清楚地观察到直肠温度的增加，并且在D49达到峰值(平均41.3℃)，然后逐渐降低直至D56。
在所有接种疫苗的组中，平均温度在整个监测期间相当稳定。当与对照组(G7)相比较时，在接种组G1、G3和G4中，最大过热在统计学上显著(p≤0.01)降低。对于G2和G6差异在统计学上不显著。
临床体征和临床评分
平均每日临床评分的演化示于图10中。每组GCS的分散描绘于图11中。
攻击后，最常观察到的临床体征是头部的充血和水肿。其中，还偶尔观察到红斑、冷漠、瘦、鼻涕及痂皮、呼吸和运动困难。
这些体征的观察的频率和持续时间在对照组中明显高于接种组。
在对照组(G7)中，平均每日临床评分在D50(即攻击后8天)达到峰值8.8，并且恒定地高于任何接种。G7的平均GCS为50。在接种组中，平均每日临床评分在整个监测期间极低。当与对照组(G7)相比较时，在除G2外的所有接种组中GCS在统计学上地显著(p≤0.01)降低。对于G2，差异接近统计显著性。
病毒血症结果
平均病毒血症滴度的演化概述于图12中。PCR阳性样品的频率和AUC示于表5中。图13示出的AUC的分散。
所有绵羊在攻击前被确认为RT-PCR阴性。在对照组中，所有绵羊在D47(即攻击后5天)为阳性，并且它们以相当高的滴度一直保持阳性，直至监测期结束。
在接种组G3和G4中，在接种后立即取样的日期，一只羊被检测具有低阳性。随后发现两组绵羊总是阴性。这些短暂的低阳性结果很可能归因于攻击接种物的残留物的检测，并且显然不归因于病毒繁殖。
当与对照组(G7)比较时，除G2外，在所有接种组中曲线下面积在统计学上显著地(p≤0.01)减少。对于G2，差异接近统计显著性。病毒血症的完全预防被认为在G1，G3，G4和G5得以实现。
表5PCR阳性样品的频率和AUC
*:商业疫苗BR8:商业BTV-8疫苗(Merial)
**:在D53死亡一只绵羊。
血清学结果
平均BTV-8中和抗体滴度的演化概述于图14中。
所有绵羊在接种前被确认为血清阴性，对照在攻击前保持血清阴性。
在2次注射接种组(G1和G4)中，在D21观察到一些血清转变(sero-conversion)。在D42，所有绵羊在两个组中具有血清转变，并且平均滴度几乎相同。
在一次注射接种组(G2、G3、G5和G6)中，在接种后21天(即D42)观察到一些血清转变。然而在一次与两次注射接种组(G1对G2和G4对G5)之间观察到血清学滴度的明显差异(D42)，根据抗原的有效负载(G2对G3)的血清学滴度的差异不太明显。
所有绵羊在攻击后(D56)发生强烈的血清转变，并且在所有接种组中存在明确的加强效应。
结论
结果表明，构建体提供过热的显著减少，临床体征的显著减少以及病毒血症的完全和显著预防。
实施例3多个血清型的合成BTV的产生
通过使用如实施例1中所述的反向遗传系统，可产生覆盖26种不同的血清型中24种的“合成”BTV重配株(sBTV)，其中每一个VP2与BTV-1主链(VP1、VP3、VP4、NS1、VP7、NS2、VP6和NS3蛋白)中的同源VP5蛋白偶联，如下面表6中显示的。术语“合成的”被用来描述该平台，其原因在于，可从已知序列体外合成基因组区段，而非通过扩增病毒株的基因组来合成基因组区段。
有趣地和令人惊讶地，尽管BTV-25(吐根堡环状病毒属(Toggenburg Orbivirus))在细胞培养不生长，但通过将BTV-1的非翻译区引入VP2和VP5蛋白的编码序列，我们就能够拯救BTV-25血清型的sBTV。因此，疫苗平台对于不能在组织培养容易分离的那些病毒株是极为有用的。
表6通过反向遗传学系统分析的可得sBTV病毒
病毒血清型代码 血清型 VP2蛋白 VP5蛋白 主链 别名<sup>+</sup>
RAS 8 BTV-01 BTV-01 BTV-01 BTV-01 <sub>S1</sub>BTV-1<sub>VP2/VP5</sub>
RAS 41 BTV-02 BTV-02 BTV-01 BTV-01 -/-
RFN 51 BTV-02 BTV-02 BTV-02 BTV-01 <sub>S1</sub>BTV-2<sub>VP2/VP5</sub>
RFN 70 BTV-02 BTV-02 BTV-02 BTV-01 -/-
RFN 30 BTV-03 BTV-03 BTV-06 BTV-01 -/-
RFN 52 BTV-03 BTV-03 BTV-03 BTV-01 <sub>S1</sub>BTV-3<sub>VP2/VP5</sub>
RAS 40 BTV-04 BTV-04 BTV-01 BTV-01 -/-
RFN 53 BTV-04 BTV-04 BTV-04 BTV-01 <sub>S1</sub>BTV-4<sub>VP2/VP5</sub>
RFN 71 BTV-04 BTV-04 BTV-04 BTV-01 -/-
RFN78 BTV-05 BTV-05 BTV-5 BTV-01 -/-
RFN 32 BTV-06 BTV-06 BTV-06 BTV-01 <sub>S1</sub>BTV-6<sub>VP2/VP5</sub>
RFN 73 BTV-07 BTV-07 BTV-01 BTV-01 -/-
RAS 32 BTV-08 BTV-08 BTV-08 BTV-01 <sub>S1</sub>BTV-8<sub>VP2/VP5</sub>
RFN 56 BTV-09 BTV-09 BTV-09 BTV-01 <sub>S1</sub>BTV-9<sub>VP2/VP5</sub>
RAS 39 BTV-09it BTV-09 BTV-01 BTV-01 -/-
RFN 74 BTV-10 BTV-10 BTV-01 BTV-01 -/-
RFN 35 BTV-11 BTV-11 BTV-01 BTV-01 -/-
RFN 58 BTV-11 BTV-11 BTV-11 BTV-01 <sub>S1</sub>BTV-11<sub>VP2/VP5</sub>
RFN 75 BTV-12 BTV-12 BTV-01 BTV-01 -/-
RFN 37 BTV-13 BTV-13 BTV-13 BTV-01 <sub>S1</sub>BTV-13<sub>VP2/VP5</sub>
RFN 38 BTV-14 BTV-14 BTV-06 BTV-01 <sub>S1</sub>BTV-14<sub>VP2</sub>
RFN76 BTV-16 BTV-16 BTV-01 BTV-01 -/-
RFN 79 BTV-16 BTV-16 BTV-10 BTV-01 -/-
RFN 80 BTV-16 BTV-16 BTV-11 BTV-01 -/-
RFN 81 BTV-16 BTV-16 BTV-16 BTV-01 -/-
RFN 82 BTV-16 BTV-16 BTV-21 BTV-01 -/-
RFN 83 BTV-16 BTV-16 BTV-22 BTV-01 -/-
RFN 84 BTV-16 BTV-16 BTV-23 BTV-01 -/-
RFN 85 BTV-16 BTV-16 BTV-26 BTV-01 -/-
RFN 41 BTV-17 BTV-17 BTV-01 BTV-01 -/-
RFN 63 BTV-17 BTV-17 BTV-17 BTV-01 <sub>S1</sub>BTV-17<sub>VP2/VP5</sub>
RFN 77 BTV-18 BTV-18 BTV-01 BTV-01 -/-
RFN 44 BTV-20 BTV-20 BTV-10 BTV-01 -/-
RFN 66 BTV-20 BTV-20 BTV-20 BTV-01 <sub>S1</sub>BTV-20<sub>VP2/VP5</sub>
RFN 45 BTV-21 BTV-21 BTV-16 BTV-01 -/-
RFN 67 BTV-21 BTV-21 BTV-21 BTV-01 <sub>S1</sub>BTV-21<sub>VP2/VP5</sub>
RFN 46 BTV-22 BTV-22 BTV-22 BTV-01 <sub>S1</sub>BTV-22<sub>VP2/VP5</sub>
RFN 47 BTV-23 BTV-23 BTV-01 BTV-01 -/-
RFN 68 BTV-23 BTV-23 BTV-23 BTV-01 <sub>S1</sub>BTV-23<sub>VP2/VP5</sub>
RFN 48 BTV-24 BTV-24 BTV-04 BTV-01 <sub>S1</sub>BTV-24<sub>VP2</sub>
RFN 49 BTV-25 BTV-25(1UTRs)<sup>#</sup> BTV-25(1UTRs)<sup>#</sup> BTV-01 <sub>S1</sub>BTV-25<sub>VP2/VP5</sub>
RFN 50 BTV-26 BTV-26 BTV-26 BTV-01 <sub>S1</sub>BTV-26<sub>VP2/VP5</sub>
#侧翼连接有BTV-1的非翻译区的蛋白质编码区
sBTV的表征和体外复制
生长每个SBTV病毒的病毒原液，且在BSR细胞中进行滴定。在感染后72小时，通过噬菌斑测定在绵羊细胞中评价噬菌斑表型(图15)。如先前所述，所有拯救的血清型具有同源VP2/VP5组合。
在BHK-21细胞(具有0.001的MOI感染)中测定sBTV复制。在感染后72小时收集细胞上清液(图16)。随后通过BHK-21细胞的TCID50滴定一式三份样品(使用Reed-Muench法)，且作为Log10TCID50/ml绘图。
实施例4灭活的重配病毒的产生
在BHK21细胞中，扩增两个构建菌株为重配BTV，一个对应于来自BTV-1主链中的BTV8的VP2，第二个具有来自BTV-1主链中的BTV8的VP2/VP5。
细胞的产生
用5升含有7％的小牛血清和2×109个细胞(6BHK2M12-第47代)的GMEM培养基接种7L生物反应器(第48代)。使用pH值：7.1±0.1，温度：37.0±1.5℃，DO2：40±10％和搅拌：300rpm±10％作为参数调节培养物。在2天的培养后，进行计数。将整个体积的悬浮液转移至预先灭菌并装有35升GMEM培养基+7％CS的B4生物反应器中。使用pH值：7.1±0.1，温度：37.0±1.5℃，DO2：40±10％和搅拌：150rpm±10％作为参数调节培养物。培养3天后，停止生物反应器的搅拌以在+5℃下约24小时的过程中倾倒细胞。倾倒后，除去培养基，(剩余体积＝5升)。添加VMM培养基(30升)。混合后，进行细胞计数，添加接种物：接种物BTV1+BTV8VP2(RAS10)(针对第一活性成分)，和接种物BTV1+BTV8 VP2/VP5(RAS32)(针对第二活性成分)。生物反应器的终体积为35升，计算接种物的体积，以获得等于5×10-4CCID50/细胞的MOI。培养的参数是：pH值：7.4±0.1，温度：37.0±1.5℃，DO2：40±10％，搅拌：100rpm±10％和压力：0.1巴±10％。在约46小时后通过在搅拌下冷却终止培养。
灭活前处理
停止氧的调节，添加氯仿以获得0.05％的终浓度。在搅拌下将混合物在约8℃放置1小时，将pH值调整至7.4±0.1。停止pH调整，在不搅拌的情况下将产物在约8℃贮存，直至处理。利用turrAX T50处理约24升培养物：在实验室温度以1000瓦处理8个3000ml的样品，进行3分钟。处理后混合样品。
随后通过离心(在20℃以3500rpm进行20分钟)澄清产物。过滤上清液，并在灭活前于+5℃贮存。
灭活
将上清液滤液在37℃进行加热，将pH调整至7.4±0.1。添加甲醛溶液以获得0.5mg/ml的终浓度。20分钟后，添加一个剂量的EI以获得1.5mM的终浓度。18小时后，加入第二剂量的EI(同一浓度)。在37℃进行灭活步骤24小时(T0＝EI的第一次添加的时间)。在该灭活步骤过程程中，利用L-半胱氨酸的溶液进行和处理样品。
过滤和浓缩
过滤(CUNO 30S)灭活产物。测量新体积。在实验室温度浓缩滤液，计算浓缩倍数(factor of concentration)。
纯化和甲醛处理
使用具有S6FF的柱子XK 25/100纯化浓缩物。进行2个运行。纯化的条件为：样品的体积：15％的柱子(约60ml)；用于洗脱的缓冲液：磷酸盐缓冲液pH7.4；速度洗脱：2.5ml/分。分别收集排阻峰(exclusion peak)(活性成分)、安全级分和蛋白质峰。将甲醛溶液添加至活性成分以获得在+5℃贮存的1mg/ml的终浓度。
BTV1+BTV8 VP2(RAS10)和BTV1+BTV8 VP2/VP5(RAS32)的感染性滴度、ELISA、斑点印迹Vp2的结果示于图17和18中。成功地进行了两个纯化灭活的BTV重配株的产生。将这两个重配株用来配制疫苗。
***********************
尽管已在本发明的详细的优选实施例进行了描述，但是应当理解，由上述段落限定的本发明不应被限于上面的描述中所示的特定细节，因为许多其显而易见的变化是可能的，并且不背离发本发明的精神或范围。
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本说明书还包括下列内容：
1.包含一种或多种重组BTV或AHSV载体的组合物或疫苗，所述载体包含编码BTV或AHSV的至少一种多肽的一个或多个异源多核苷酸，其中所述重组BTV或AHSV载体包含来源于选自BTV或AHSV的血清型1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25和26的基因组的载体主链。
2.实施方式1的组合物，其中所述多肽选自BTV或AHSV的VP1、VP2、VP3、VP4、VP5、VP7、NS1、NS2、VP6和NS3/3A。
3.实施方式1或2的组合物，其中所述多肽来自BTV-8并且所述载体主链来源于BTV-1基因组。
4.实施方式1或2的组合物，其中所述多肽来自BTV-1并且所述载体主链来源于BTV-8基因组。
5.实施方式3的组合物，其中所述多肽来自BTV-8的VP2并且所述载体主链来源于BTV-1。
6.实施方式3的组合物，其中所述多肽为BTV-8的VP5并且所述载体主链来源于BTV-1。
7.实施方式3的组合物，其中所述多肽为BTV-8的VP2和VP5并且所述载体主链来源于BTV-1。
8.实施方式3的组合物，其中所述多肽为BTV-1的VP2并且所述载体主链来源于BTV-8。
9.实施方式4的组合物，其中所述多肽为BTV-1的VP5并且所述载体主链来源于BTV-8。
10.实施方式4的组合物，其中所述多肽为BTV-1的VP2和VP5并且所述载体主链来源于BTV-8。
11.重组BTV或AHSV载体，所述载体包含编码BTV或AHSV的至少一种多肽的一个或多个异源多核苷酸，其中所述重组BTV或AHSV载体包含载体主链，所述主链包含选自BTV或AHSV的血清型1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25和26的基因组。
12.实施方式11的重组BTV或AHSV载体，其中所述多肽选自BTV或AHSV的VP1、VP2、VP3、VP4、VP5、VP7、NS1、NS2、VP6和NS3/3A。
13.实施方式11或12的重组BTV或AHSV载体，其中所述多肽来自BTV-8并且所述载体主链来源于BTV-1基因组。
14.实施方式11或12的重组BTV或AHSV载体，其中所述多肽来自BTV-1并且所述载体主链来源于BTV-8基因组。
15.实施方式13的重组BTV或AHSV载体，其中所述多肽为BTV-8的VP2并且所述载体主链来源于BTV-1。
16.实施方式13的重组BTV或AHSV载体，其中所述多肽为BTV-8的VP5并且所述载体主链来源于BTV-1。
17.实施方式13的重组BTV或AHSV载体，其中所述多肽为BTV-8的VP2和VP5并且所述载体主链来源于BTV-1。
18.实施方式14的重组BTV或AHSV载体，其中所述多肽为BTV-1的VP2并且所述载体主链来源于BTV-8。
19.实施方式14的重组BTV或AHSV载体，其中所述多肽为BTV-1的VP5并且所述载体主链来源于BTV-9。
20.实施方式14的重组BTV或AHSV载体，其中所述多肽为BTV-1的VP2和VP5并且所述载体主链来源于BTV-8。
21.一种产生重组BTV或AHSV载体的方法，其包括用如下RNA转染细胞：
a)编码BTV或AHSV的血清型的一种或多种多肽的RNA；和
b)包含与a)中的BTV或ASHV的血清型不同的BTV或AHSV血清型的完整基因组的转录物的RNA，和包括编码a)中的多肽的转录物缺失的RNA。
22.实施方式21的方法，其中a)中的RNA编码BTV-8的VP2，并且其中b)中的RNA包含(编码)BTV-1的VP1、VP3、VP4、VP5、VP7、NS1、NS2、VP6NS4和NS3/3A的转录物。
23.实施方式21的方法，其中a)中的RNA编码BTV-8的VP5，并且其中b)中的RNA包含(编码)BTV-1的VP1、VP2、VP3、VP4、VP7、NS1、NS2、VP6NS4和NS3/3A的转录物。
24.实施方式21的方法，其中a)中的RNA编码BTV-8的VP2和VP5，并且其中b)中的RNA包含(编码)BTV-1的VP1、VP3、VP4、VP7、NS1、NS2、VP6NS4和NS3/3A的转录物。
25.实施方式21的方法，其中a)中的RNA编码BTV-1的VP2，并且其中b)中的RNA包含(编码)BTV-8的VP1、VP3、VP4、VP5、VP7、NS1、NS2、VP6NS4和NS3/3A的转录物。
26.实施方式21的方法，其中a)中的RNA编码BTV-1的VP5，并且其中b)中的RNA包含(编码)BTV-8的VP1、VP3、VP4、VP5、VP7、NS1、NS2、VP6NS4和NS3/3A的转录物。
27.实施方式21的方法，其中a)中的RNA编码BTV-1的VP2和VP5，并且其中b)中的RNA包含(编码)BTV-8的VP1、VP3、VP4、VP7、NS1、NS2、VP6NS4和NS3/3A的转录物。
28.一种用于接种动物的方法，其包括至少一次施用实施方式1至20的任一项的组合物或载体。
29.实施方式28的方法，其中所述方法包括初免-加强施用方案。
30.一种用于诱导动物抗一种或多种病原体的免疫原性或保护性应答的方法，其包括至少一次施用实施方式1至20的任一项的组合物或载体。
31.实施方式30的方法，其中所述动物为牛类动物、绵羊类动物、山羊类动物或马类动物。
序列表
<110> Palmarini, Massimo
Nunes, Sandro
Hudelet, Pascal
Audonnet, Jean-Christophe
<120> 重配BTV和AHSV疫苗
<130> MER 12-200.PCT
<150> 61/659,198
<151> 2012-06-13
<160> 194
<170> PatentIn version 3.5
<210> 1
<211> 1302
<212> PRT
<213> 人工序列
<220>
<223> BTV-1 ACR58458的VP1
<400> 1
Met Val Ala Ile Thr Val Gln Gly Ala Gln Leu Ile Lys Arg Val Val
1 5 10 15
Glu Arg Phe Tyr Pro Gly Ile Ala Phe Asn Ile Asp Glu Gly Ala Cys
20 25 30
Tyr Ile Tyr Lys Phe Ser Asp His Ile Arg Arg Ile Arg Met Lys His
35 40 45
Gly Thr Lys Tyr Arg Arg Gln Ala Glu Glu Ile Met Arg Asn Ile Ser
50 55 60
Leu Arg Lys Glu Arg Leu Tyr Gly Ile Pro Val Leu Asp Glu Val Glu
65 70 75 80
Trp Lys Tyr Val Phe Asp Gly Gln Thr Phe Gln Ser Tyr Ala Phe Glu
85 90 95
Val Tyr Val Asn Ser Ile Leu Pro Trp Ser Glu Leu Asp Pro Glu Glu
100 105 110
Glu Phe Leu Arg Asn Tyr Arg Val Ser Arg Glu Met Thr Glu Val Glu
115 120 125
Lys Phe Ile Glu Phe Arg Ala Lys Asn Glu Met Gln Ile Tyr Gly Asp
130 135 140
Ile Pro Ile Lys Val Trp Cys Cys Phe Ile Asn Glu Leu Ser Ala Glu
145 150 155 160
Leu Lys His Ile Pro Leu Gly Met Gln Val Met Ala Asp Phe Val Asn
165 170 175
Arg Phe Asp Ser Pro Phe His Gln Gly Asn Arg Asp Leu Ser Asn Leu
180 185 190
Glu Asp Phe Gln Val Ala Tyr Thr Thr Pro Leu Leu Phe Glu Met Cys
195 200 205
Cys Met Glu Ser Ile Leu Glu Phe Asn Ile Lys Met Arg Met Arg Glu
210 215 220
Glu Asp Ile Ser Ala Leu Glu Phe Gly Asp Met Lys Val Asp Pro Val
225 230 235 240
Gly Leu Leu Arg Glu Phe Phe Ile Leu Cys Leu Pro His Pro Lys Lys
245 250 255
Ile Asn Asn Val Leu Arg Ala Pro Tyr Ser Trp Phe Val Lys Met Trp
260 265 270
Gly Val Gly Ala Asp Pro Ile Val Val Leu Gln Ser Thr Ala Gly Asp
275 280 285
Asp Arg Asn Ser Lys Asp Val Phe Tyr Asp Lys Phe Arg Thr Glu Pro
290 295 300
Asn Arg Tyr Lys Ala Leu Phe Arg Ser Ser Phe Tyr Asn Glu Ser Arg
305 310 315 320
Arg Met Asn Glu Glu Lys Ile Leu Glu Ala Val Lys Tyr Ser Gln Lys
325 330 335
Leu Gly Ser His Glu Arg Arg Leu Pro Leu Phe Glu Lys Met Leu Lys
340 345 350
Thr Val Tyr Thr Thr Pro Phe Tyr Pro His Lys Ser Ser Asn Met Ile
355 360 365
Leu Ala Ser Phe Leu Leu Ser Ile Gln Thr Ile Thr Gly Tyr Gly Arg
370 375 380
Ala Trp Val Lys Asn Val Ser Thr Glu Phe Asp Lys Gln Leu Lys Pro
385 390 395 400
Asn Pro Ser Asn Leu Val Gln Asp Val Ser Asp Leu Thr Arg Glu Phe
405 410 415
Phe Lys Gln Ala Tyr Val Glu Ala Lys Glu Arg Arg Glu Glu Ile Val
420 425 430
Lys Pro Glu Asp Leu Tyr Thr Ser Met Leu Arg Leu Ala Arg Asn Thr
435 440 445
Ser Ser Gly Phe Ser Thr Glu Ile Tyr Val Lys Lys Arg Phe Gly Pro
450 455 460
Arg Leu Arg Asp Lys Asp Leu Ile Lys Ile Asn Ser Arg Ile Lys Ala
465 470 475 480
Leu Val Ile Phe Thr Lys Gly His Thr Val Phe Thr Asp Glu Glu Leu
485 490 495
His Lys Lys Tyr Asn Ser Val Glu Leu Tyr Gln Thr Lys Gly Ser Arg
500 505 510
Asp Val Pro Ile Lys Ala Thr Arg Thr Ile Tyr Ser Ile Asn Leu Ser
515 520 525
Val Leu Val Pro Gln Leu Ile Val Thr Leu Pro Leu Asn Glu Tyr Phe
530 535 540
Ser Arg Val Gly Gly Ile Thr Ser Pro Asp Tyr Lys Lys Ile Gly Gly
545 550 555 560
Lys Val Ile Val Gly Asp Leu Glu Ala Thr Gly Ser Arg Val Met Asp
565 570 575
Ala Ala Asp Cys Phe Arg Asn Ser Ala Asp Arg Asp Ile Phe Thr Ile
580 585 590
Ala Ile Asp Tyr Ser Glu Tyr Asp Thr His Leu Thr Arg His Asn Phe
595 600 605
Arg Thr Gly Met Leu Gln Gly Ile Arg Glu Ala Met Ala Pro Tyr Arg
610 615 620
Asp Leu Arg Tyr Glu Gly Tyr Thr Leu Glu Gln Ile Ile Asp Phe Gly
625 630 635 640
Tyr Gly Glu Gly Arg Val Ala Asn Thr Leu Trp Asn Gly Lys Arg Arg
645 650 655
Leu Phe Lys Thr Thr Phe Asp Ala Tyr Ile Arg Leu Asp Glu Ser Glu
660 665 670
Arg Asp Lys Gly Ser Phe Lys Val Pro Lys Gly Val Leu Pro Val Ser
675 680 685
Ser Ile Asp Val Ala Asn Arg Ile Ala Val Asp Lys Gly Phe Asp Thr
690 695 700
Leu Ile Ala Ala Thr Asp Gly Ser Asp Leu Ala Leu Ile Asp Thr His
705 710 715 720
Leu Ser Gly Glu Asn Ser Thr Leu Ile Ala Asn Ser Met His Asn Met
725 730 735
Ala Ile Gly Thr Leu Ile Gln Arg Glu Val Gly Lys Glu Gln Pro Gly
740 745 750
Ile Leu Thr Phe Leu Ser Glu Gln Tyr Val Gly Asp Asp Thr Leu Phe
755 760 765
Tyr Thr Lys Leu His Thr Thr Asp Thr Lys Val Phe Asp Lys Val Ala
770 775 780
Ala Ser Ile Phe Asn Thr Val Ala Lys Cys Gly His Glu Ala Ser Pro
785 790 795 800
Ser Lys Thr Met Met Thr Pro Tyr Ser Val Glu Lys Thr Gln Thr His
805 810 815
Ala Lys Gln Gly Cys Tyr Val Pro Gln Asp Arg Met Met Ile Ile Ser
820 825 830
Ser Glu Arg Arg Lys Asp Ile Glu Asp Val Gln Gly Tyr Val Arg Ser
835 840 845
Gln Val Gln Thr Met Ile Thr Lys Val Ser Arg Gly Phe Cys His Asp
850 855 860
Leu Ala Gln Leu Ile Leu Met Leu Lys Thr Thr Phe Ile Gly Ala Trp
865 870 875 880
Lys Met Lys Arg Thr Ile Lys Glu Asp Ala Met Tyr Arg Asp Arg Lys
885 890 895
Phe Asp Ser Asn Asp Glu Asp Gly Phe Thr Leu Ile Gln Ile Arg Asn
900 905 910
Pro Leu Ala Leu Tyr Val Pro Ile Gly Trp Asn Gly Tyr Gly Ala His
915 920 925
Pro Ala Ala Leu Asn Ile Val Met Thr Glu Glu Met Tyr Val Asp Ser
930 935 940
Ile Met Ile Ser Lys Leu Asp Glu Ile Met Ala Pro Ile Arg Arg Ile
945 950 955 960
Val His Asp Ile Pro Pro Cys Trp Asn Glu Thr Gln Gly Asp Lys Arg
965 970 975
Gly Leu Ile Ser Ala Thr Lys Met Ser Phe Phe Ser Lys Met Ala Arg
980 985 990
Pro Ala Val Gln Ala Ala Leu Ser Asp Pro Gln Ile Met Asn Leu Val
995 1000 1005
Glu Glu Leu Pro Leu Gly Glu Phe Ser Pro Gly Arg Ile Ser Arg
1010 1015 1020
Thr Met Met His Ser Ala Leu Leu Lys Glu Ser Ser Ala Arg Thr
1025 1030 1035
Leu Leu Ser Ser Gly Tyr Glu Leu Glu Tyr Gln Lys Ala Leu Asn
1040 1045 1050
Ser Trp Ile Ala Gln Val Ser Met Arg Leu Gly Glu Glu Ser Gly
1055 1060 1065
Val Ile Ser Thr Ser Tyr Ala Lys Leu Phe Asp Val Tyr Phe Glu
1070 1075 1080
Gly Glu Leu Asp Gly Ala Pro Tyr Met Phe Pro Asp Gln Asn Leu
1085 1090 1095
Ser Pro Gln Phe Tyr Ile Gln Lys Met Met Ile Gly Pro Arg Val
1100 1105 1110
Ser Ser Arg Val Arg Asn Ser Tyr Val Asp Arg Ile Asp Val Ile
1115 1120 1125
Leu Arg Lys Asp Val Val Met Arg Gly Phe Ile Thr Ala Asn Thr
1130 1135 1140
Ile Leu Asn Val Ile Glu Lys Leu Gly Thr Asn His Ser Val Gly
1145 1150 1155
Asp Leu Val Thr Val Phe Thr Leu Met Asn Ile Glu Thr Arg Val
1160 1165 1170
Ala Glu Glu Leu Ala Glu Tyr Met Thr Ser Glu Lys Ile Arg Phe
1175 1180 1185
Asp Ala Leu Lys Leu Leu Lys Lys Gly Ile Ala Gly Asp Glu Phe
1190 1195 1200
Thr Met Ser Leu Asn Val Ala Thr Gln Asp Phe Ile Asp Thr Tyr
1205 1210 1215
Leu Ala Tyr Pro Tyr Gln Leu Thr Lys Thr Glu Val Asp Ala Ile
1220 1225 1230
Ser Leu Tyr Cys Thr Gln Met Val Met Leu Arg Ala Ala Leu Gly
1235 1240 1245
Leu Pro Lys Lys Lys Met Lys Ile Val Val Thr Asp Asp Ala Lys
1250 1255 1260
Lys Arg Tyr Lys Ile Arg Leu Gln Arg Phe Arg Thr His Val Pro
1265 1270 1275
Lys Ile Lys Val Leu Lys Lys Leu Ile Asp Pro Asn Arg Met Thr
1280 1285 1290
Val Arg Asn Leu Glu Asn Gln Phe Val
1295 1300
<210> 2
<211> 961
<212> PRT
<213> 人工序列
<220>
<223> BTV1 ACR58459的VP2
<400> 2
Met Asp Glu Leu Gly Ile Pro Val Tyr Lys Arg Gly Phe Pro Glu His
1 5 10 15
Leu Leu Arg Gly Tyr Glu Phe Ile Ile Asp Val Gly Thr Lys Ile Glu
20 25 30
Ser Val Gly Gly Arg His Asp Val Thr Lys Ile Pro Glu Met Asn Ala
35 40 45
Tyr Asp Ile Lys Gln Glu Ser Ile Arg Thr Ala Leu Trp Tyr Asn Pro
50 55 60
Ile Arg Asn Asp Gly Phe Val Leu Pro Arg Val Leu Asp Ile Thr Leu
65 70 75 80
Arg Gly Tyr Asp Glu Arg Arg Ala Val Val Glu Ser Thr Arg His Lys
85 90 95
Ser Phe His Thr Asn Asp Gln Trp Val Gln Trp Met Met Lys Asp Ser
100 105 110
Met Asp Ala Gln Pro Leu Lys Val Gly Leu Asp Asp Gln Ser Arg Asn
115 120 125
Val Ala His Ser Leu His Asn Cys Val Val Lys Ile Asp Ser Lys Lys
130 135 140
Ala Asp Thr Met Ser Tyr His Val Glu Pro Ile Glu Asp Ala Ser Lys
145 150 155 160
Gly Cys Leu His Thr Arg Thr Met Met Trp Asn His Leu Val Arg Ile
165 170 175
Glu Thr Phe His Ala Ala Gln Glu Val Ala Tyr Thr Leu Lys Pro Thr
180 185 190
Tyr Asp Ile Val Val His Ala Glu Arg Arg Asp Arg Ser Gln Pro Phe
195 200 205
Arg Pro Gly Asp Gln Thr Leu Ile Asn Phe Gly Arg Gly Gln Lys Val
210 215 220
Ala Met Asn His Asn Ser Tyr Asp Lys Met Val Glu Gly Leu Thr His
225 230 235 240
Leu Val Ile Arg Gly Lys Thr Pro Glu Val Ile Arg Asp Asp Ile Ala
245 250 255
Ser Leu Asp Glu Ile Cys Asn Arg Trp Ile Gln Ser Arg His Asp Pro
260 265 270
Gly Glu Ile Lys Ala Tyr Glu Leu Cys Lys Ile Leu Ser Thr Ile Gly
275 280 285
Arg Lys Val Leu Asp Arg Glu Lys Glu Pro Glu Asp Glu Ala Asn Leu
290 295 300
Ser Ile Arg Phe Gln Glu Ala Ile Asp Asn Lys Phe Arg Gln His Asp
305 310 315 320
Pro Glu Arg Leu Lys Ile Phe Glu His Arg Asn Gln Arg Arg Asp Glu
325 330 335
Asp Arg Phe Tyr Ile Leu Leu Met Ile Ala Ala Ser Asp Thr Phe Asn
340 345 350
Thr Arg Val Trp Trp Ser Asn Pro Tyr Pro Cys Leu Arg Gly Thr Leu
355 360 365
Ile Ala Ser Glu Thr Lys Leu Gly Asp Val Tyr Ser Met Met Arg Ser
370 375 380
Trp Tyr Asp Trp Ser Val Arg Pro Thr Tyr Thr Pro Tyr Glu Lys Thr
385 390 395 400
Arg Glu Gln Glu Lys Tyr Ile Tyr Gly Arg Val Asn Leu Phe Asp Phe
405 410 415
Val Ala Glu Pro Gly Ile Lys Ile Val His Trp Glu Tyr Arg Leu Asn
420 425 430
His Ser Thr Arg Glu Ile Thr Tyr Ala Gln Gly Asn Pro Cys Asp Leu
435 440 445
Tyr Pro Glu Asp Asp Asp Val Ile Val Thr Lys Phe Asp Asp Val Ala
450 455 460
Tyr Gly Gln Met Ile Asn Glu Met Ile Asn Gly Gly Trp Asn Gln Glu
465 470 475 480
Gln Phe Lys Met His Lys Ile Leu Lys Ser Glu Gly Asn Val Leu Thr
485 490 495
Ile Asp Phe Glu Lys Asp Ala Lys Leu Thr Thr Asn Glu Gly Val Thr
500 505 510
Met Pro Glu Tyr Phe Asn Lys Trp Ile Ile Ala Pro Met Phe Asn Ala
515 520 525
Lys Leu Arg Ile Lys His Glu Glu Ile Ala Gln Arg Gln Ser Asp Asp
530 535 540
Pro Met Val Lys Arg Thr Leu Ser Pro Ile Thr Ala Asp Pro Ile Glu
545 550 555 560
Leu Gln Arg Leu Thr Leu Ala Arg Phe Tyr Asp Ile Arg Pro Ala Leu
565 570 575
Arg Gly Gln Ala Leu Ser Arg Gln Gln Ala Gln Ser Thr Tyr Asp Glu
580 585 590
Glu Ile Ser Lys Gln Arg Asp Tyr Ala Glu Ile Leu Lys Arg Arg Gly
595 600 605
Ile Val Gln Ile Pro Lys Lys Pro Cys Pro Thr Val Thr Ala Gln Tyr
610 615 620
Thr Leu Glu Arg Tyr Ala Leu Phe Ile Ile Asn Ile Leu Gln Gln His
625 630 635 640
Val Ala Arg Asp Cys Asp Glu Glu Ala Ile Tyr Glu His Pro Lys Ala
645 650 655
Asp Tyr Glu Leu Glu Ile Phe Gly Glu Ser Ile Val Asp Ile Ser Gln
660 665 670
Val Ile Val Leu Val Phe Asp Leu Ile Phe Glu Arg Arg Arg Arg Val
675 680 685
Arg Asp Val Tyr Glu Ser Arg Tyr Ile Ile Ala Arg Ile Arg Arg Met
690 695 700
Arg Gly Lys Glu Arg Leu Asn Val Ile Ala Glu Phe Phe Pro Thr Tyr
705 710 715 720
Gly Ser Leu Leu Asn Gly Leu Asn Ser Ala Thr Val Val Gln Asp Ile
725 730 735
Met Tyr Leu Asn Phe Leu Pro Leu Tyr Phe Leu Ala Gly Asp Asn Met
740 745 750
Ile Tyr Ser His Arg Gln Trp Ser Ile Pro Leu Leu Leu Tyr Thr His
755 760 765
Glu Val Met Val Val Pro Leu Glu Val Gly Ser Tyr Asn Asp Arg Cys
770 775 780
Gly Leu Ile Ala Tyr Leu Glu Tyr Met Val Phe Phe Pro Ser Lys Ala
785 790 795 800
Ile Arg Leu Ser Lys Leu Asn Glu Ala Gln Pro Lys Ile Ala Arg Glu
805 810 815
Met Leu Lys Tyr Tyr Ala Asn Thr Ala Val Tyr Asp Gly Gly Val Asn
820 825 830
Tyr Asn Val Val Thr Thr Lys Gln Leu Leu Tyr Glu Thr Tyr Leu Ala
835 840 845
Ser Leu Cys Gly Gly Ile Ser Asp Gly Ile Val Trp Tyr Leu Pro Ile
850 855 860
Thr His Pro Asn Lys Cys Ile Val Ala Ile Glu Val Ser Asp Glu Arg
865 870 875 880
Val Pro Ala Ser Ile Arg Ala Gly Arg Ile Arg Leu Arg Phe Pro Leu
885 890 895
Ser Ala Arg His Leu Lys Gly Val Val Ile Ile Gln Ile Asp Glu Glu
900 905 910
Gly Glu Phe Thr Val Tyr Ser Glu Gly Ile Val Ser His Arg Val Cys
915 920 925
Lys Lys Asn Leu Leu Lys Tyr Met Cys Asp Ile Ile Leu Leu Lys Phe
930 935 940
Ser Gly His Val Phe Gly Asn Asp Glu Met Leu Thr Lys Leu Leu Asn
945 950 955 960
Val
<210> 3
<211> 901
<212> PRT
<213> 人工序列
<220>
<223> BTV1 ACR58460的VP3
<400> 3
Met Ala Ala Gln Asn Glu Gln Arg Pro Glu Arg Ile Lys Thr Thr Pro
1 5 10 15
Tyr Leu Glu Gly Asp Val Leu Ser Ser Asp Ser Gly Pro Leu Leu Ser
20 25 30
Val Phe Ala Leu Gln Glu Ile Met Gln Lys Val Arg Gln Val Gln Ala
35 40 45
Asp Tyr Met Thr Ala Thr Arg Glu Val Asp Phe Thr Val Pro Asp Val
50 55 60
Gln Lys Ile Leu Asp Asp Ile Lys Ala Leu Ala Ala Glu Gln Val Tyr
65 70 75 80
Lys Ile Val Lys Val Pro Ser Ile Ser Phe Arg His Ile Val Met Gln
85 90 95
Ser Arg Asp Arg Val Leu Arg Val Asp Thr Tyr Tyr Glu Glu Met Ser
100 105 110
Gln Val Gly Asp Val Ile Thr Glu Asp Glu Pro Glu Lys Phe Tyr Ser
115 120 125
Thr Ile Ile Lys Lys Val Arg Phe Ile Arg Gly Lys Gly Ser Phe Ile
130 135 140
Leu His Asp Ile Pro Thr Arg Asp His Arg Gly Met Glu Val Ala Glu
145 150 155 160
Pro Glu Val Leu Gly Val Glu Phe Lys Asn Val Leu Pro Val Leu Thr
165 170 175
Ala Glu His Arg Ala Met Ile Gln Asn Ala Leu Asp Gly Ser Ile Ile
180 185 190
Glu Asn Gly Asn Val Ala Thr Arg Asp Val Asp Val Phe Ile Gly Ala
195 200 205
Cys Ser Glu Pro Ile Tyr Arg Ile Tyr Asn Arg Leu Gln Gly Tyr Ile
210 215 220
Glu Ala Val Gln Leu Gln Glu Leu Arg Asn Ser Ile Gly Trp Leu Glu
225 230 235 240
Arg Leu Gly Gln Arg Lys Arg Ile Thr Tyr Ser Gln Glu Val Leu Thr
245 250 255
Asp Phe Arg Arg Gln Asp Thr Ile Trp Val Leu Ala Leu Gln Leu Pro
260 265 270
Val Asn Pro Gln Val Val Trp Asp Val Pro Arg Ser Ser Ile Ala Asn
275 280 285
Leu Ile Met Asn Ile Ala Thr Cys Leu Pro Thr Gly Glu Tyr Ile Ala
290 295 300
Pro Asn Pro Arg Ile Ser Ser Ile Thr Leu Thr Gln Arg Ile Thr Thr
305 310 315 320
Thr Gly Pro Phe Ala Ile Leu Thr Gly Ser Thr Pro Thr Ala Gln Gln
325 330 335
Leu Asn Asp Val Arg Lys Ile Tyr Leu Ala Leu Met Phe Pro Gly Gln
340 345 350
Ile Ile Leu Asp Leu Lys Ile Asp Pro Gly Glu Arg Met Asp Pro Ala
355 360 365
Val Arg Met Val Ala Gly Val Val Gly His Leu Leu Phe Thr Ala Gly
370 375 380
Gly Arg Phe Thr Asn Leu Thr Gln Asn Met Ala Arg Gln Leu Asp Ile
385 390 395 400
Ala Leu Asn Asp Tyr Leu Leu Tyr Met Tyr Asn Thr Arg Val Gln Val
405 410 415
Asn Tyr Gly Pro Thr Gly Glu Pro Leu Asp Phe Gln Ile Gly Arg Asn
420 425 430
Gln Tyr Asp Cys Asn Val Phe Arg Ala Asp Phe Ala Thr Gly Thr Gly
435 440 445
Tyr Asn Gly Trp Ala Thr Ile Asp Val Glu Tyr Arg Asp Pro Ala Pro
450 455 460
Tyr Val His Ala Gln Arg Tyr Ile Arg Tyr Cys Gly Ile Asp Ser Arg
465 470 475 480
Glu Leu Ile Asn Pro Thr Thr Tyr Gly Ile Gly Met Thr Tyr His Cys
485 490 495
Tyr Asn Glu Met Leu Arg Met Leu Val Ala Ala Gly Lys Asp Ser Glu
500 505 510
Ala Ala Tyr Phe Arg Ser Met Leu Pro Phe His Met Val Arg Phe Ala
515 520 525
Arg Ile Asn Gln Ile Ile Asn Glu Asp Leu His Ser Val Phe Ser Leu
530 535 540
Pro Asp Asp Met Phe Asn Ala Leu Leu Pro Asp Leu Ile Ala Gly Ala
545 550 555 560
His Gln Asn Ala Asp Pro Val Val Leu Asp Val Ser Trp Ile Ser Leu
565 570 575
Trp Phe Ala Phe Asn Arg Ser Phe Glu Pro Thr His Arg Asn Glu Met
580 585 590
Leu Glu Ile Ala Pro Leu Ile Glu Ser Val Tyr Ala Ser Glu Leu Ser
595 600 605
Val Met Lys Val Asp Met Arg His Leu Ser Leu Met Gln Arg Arg Phe
610 615 620
Pro Asp Val Leu Ile Gln Ala Arg Pro Ser His Phe Trp Lys Ala Val
625 630 635 640
Leu Asn Asp Ser Pro Glu Ala Val Lys Ala Val Met Asn Leu Ser His
645 650 655
Ser His Asn Phe Ile Asn Ile Arg Asp Met Met Arg Trp Val Leu Leu
660 665 670
Pro Ser Leu Gln Pro Ser Leu Lys Leu Val Leu Glu Glu Glu Ala Trp
675 680 685
Ala Ala Ala Asn Asp Phe Glu Asp Leu Met Leu Thr Asp Gln Val Tyr
690 695 700
Met His Arg Asp Met Leu Pro Glu Pro Arg Leu Asp Asp Ile Glu Arg
705 710 715 720
Phe Arg Gln Glu Gly Phe Tyr Tyr Thr Asn Met Leu Glu Ala Pro Pro
725 730 735
Glu Ile Asp Arg Val Val Gln Tyr Thr Tyr Glu Ile Ala Arg Leu Gln
740 745 750
Ala Asn Met Gly Gln Phe Arg Ala Ala Leu Arg Arg Ile Met Asp Asp
755 760 765
Asp Asp Trp Val Arg Phe Gly Gly Val Leu Arg Thr Val Arg Val Lys
770 775 780
Phe Phe Asp Ala Arg Pro Pro Asp Asp Ile Leu Gln Gly Leu Pro Phe
785 790 795 800
Ser Tyr Asp Thr Asn Glu Lys Gly Gly Leu Ser Tyr Ala Thr Ile Lys
805 810 815
Tyr Ala Thr Glu Thr Thr Ile Phe Tyr Leu Ile Tyr Asn Val Glu Phe
820 825 830
Ser Asn Thr Pro Asp Ser Leu Val Leu Ile Asn Pro Thr Tyr Thr Met
835 840 845
Thr Lys Val Phe Ile Asn Lys Arg Ile Val Glu Arg Val Arg Val Gly
850 855 860
Gln Ile Leu Ala Val Leu Asn Arg Arg Phe Val Ala Tyr Lys Gly Lys
865 870 875 880
Met Arg Ile Met Asp Ile Thr Gln Ser Leu Lys Met Gly Thr Lys Leu
885 890 895
Ala Ala Pro Thr Val
900
<210> 4
<211> 644
<212> PRT
<213> 人工序列
<220>
<223> BTV1 ACR58461的VP4
<400> 4
Met Pro Glu Pro His Ala Val Leu Tyr Val Thr Asn Glu Leu Ser His
1 5 10 15
Ile Val Lys Ser Gly Phe Leu Pro Ile Trp Arg Leu Thr Gly Asp Glu
20 25 30
Ser Leu Asn Asp Leu Trp Leu Glu Asn Gly Lys Tyr Ala Thr Asp Val
35 40 45
Tyr Ala Tyr Gly Asp Val Ser Lys Trp Thr Ile Arg Gln Leu Arg Gly
50 55 60
His Gly Phe Ile Phe Val Ser Thr His Lys Asn Ile Gln Leu Ala Asp
65 70 75 80
Ile Ile Lys Thr Val Asp Val Arg Ile Pro Arg Glu Val Ala Lys Ser
85 90 95
Gln Asp Met Lys Ala Phe Glu Asn Glu Ile Gly Arg Arg Arg Ile Arg
100 105 110
Met Arg Lys Gly Phe Gly Asp Ala Leu Arg Asn Tyr Ala Phe Lys Met
115 120 125
Ala Ile Glu Phe His Gly Ser Glu Ala Glu Thr Leu Asn Asp Ala Asn
130 135 140
Pro Arg Leu His Lys Val Tyr Gly Met Pro Glu Ile Pro Pro Leu Tyr
145 150 155 160
Met Glu Tyr Ala Glu Ile Gly Thr Arg Phe Asp Asp Glu Pro Thr Asp
165 170 175
Glu Lys Leu Val Ser Met Leu Asp Tyr Ile Val Tyr Ser Ala Glu Glu
180 185 190
Val His Tyr Val Gly Cys Gly Asp Leu Arg Thr Leu Met Gln Phe Lys
195 200 205
Lys Arg Ser Pro Gly Arg Phe Arg Arg Val Leu Trp His Val Tyr Asp
210 215 220
Pro Ile Ala Pro Glu Ser Ser Asp Pro Asn Val Ile Val His Asn Val
225 230 235 240
Met Val Asp Ser Lys Lys Asp Ile Leu Lys His Met Asn Phe Leu Lys
245 250 255
Arg Val Glu Arg Leu Phe Ile Trp Asp Val Ser Ser Asp Arg Ser Gln
260 265 270
Met Asp Asp Asp Glu Trp Glu Ser Thr Arg Phe Ala Glu Asp Arg Leu
275 280 285
Gly Glu Glu Ile Ala Tyr Glu Met Gly Gly Ala Phe Ser Ser Ala Leu
290 295 300
Ile Lys His Arg Ile Pro Asn Ser Arg Asp Glu Tyr His Cys Ile Ser
305 310 315 320
Thr Tyr Leu Leu Pro Gln Pro Gly Ala Asp Ala Asp Met Tyr Glu Leu
325 330 335
Arg Asn Phe Met Arg Leu Lys Gly Tyr Ser His Val Asp Arg His Met
340 345 350
His Pro Asp Ala Ser Val Met Lys Val Val Ser Arg Asp Val Arg Lys
355 360 365
Met Val Glu Leu Tyr His Gly Arg Asp Arg Gly Arg Phe Leu Lys Lys
370 375 380
Arg Leu Phe Glu His Leu His Ile Val Arg Lys Asn Gly Leu Leu His
385 390 395 400
Glu Ser Asp Glu Pro Arg Ala Asp Leu Phe Tyr Leu Thr Asn Arg Cys
405 410 415
Asn Met Gly Leu Glu Pro Ser Ile Tyr Glu Val Met Lys Lys Ser Val
420 425 430
Ile Ala Thr Ala Trp Val Gly Arg Ala Pro Leu Tyr Asp Tyr Asp Asp
435 440 445
Tyr Ala Leu Pro Arg Ser Thr Val Met Leu Asn Gly Ser Tyr Arg Asp
450 455 460
Ile Arg Val Leu Asp Gly Asn Gly Ala Ile Leu Phe Leu Met Trp Lys
465 470 475 480
Tyr Pro Asp Ile Val Lys Lys Asp Leu Thr Tyr Asp Pro Ala Trp Ala
485 490 495
Met Asn Phe Ala Val Ser Leu Lys Glu Pro Ile Pro Asp Pro Pro Val
500 505 510
Pro Asp Ile Ser Leu Cys Arg Phe Ile Gly Leu Arg Val Glu Ser Ser
515 520 525
Val Leu Arg Val Arg Asn Pro Thr Leu His Glu Thr Ala Asp Glu Leu
530 535 540
Lys Arg Met Gly Leu Asp Leu Ser Gly His Leu Tyr Val Thr Leu Met
545 550 555 560
Ser Gly Ala Tyr Val Thr Asp Leu Phe Trp Trp Phe Lys Met Ile Leu
565 570 575
Asp Trp Ser Ala Gln Ser Lys Glu Gln Lys Leu Arg Asp Leu Lys Arg
580 585 590
Ser Ala Ala Glu Val Ile Glu Trp Lys Glu Gln Met Ala Glu Arg Pro
595 600 605
Trp His Val Arg Asn Asp Leu Ile Ala Ala Leu Arg Glu Tyr Lys Arg
610 615 620
Lys Met Gly Met Arg Glu Gly Ala Ser Ile Asp Ser Trp Leu Glu Leu
625 630 635 640
Leu Arg His Leu
<210> 5
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV1 ACR58462的BP5
<400> 5
Met Gly Lys Val Ile Arg Ser Leu Asn Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ser
65 70 75 80
Gly Glu Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Ala Lys Leu Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Asp Lys Ile Lys Glu Lys Phe Gly Lys Glu Leu Glu
115 120 125
Glu Val Tyr Asn Phe Met Asn Gly Glu Ala Asn Ala Glu Ile Glu Asp
130 135 140
Glu Lys Gln Phe Asp Ile Leu Asn Lys Ala Val Thr Ser Tyr Asn Lys
145 150 155 160
Ile Leu Thr Glu Glu Asp Leu Gln Met Arg Arg Leu Ala Thr Ala Leu
165 170 175
Gln Lys Glu Ile Gly Glu Arg Thr His Ala Glu Thr Val Met Val Lys
180 185 190
Glu Tyr Arg Asp Lys Ile Asp Ala Leu Lys Asn Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Thr His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Ser Val Val Ser Thr Ile Leu Glu
275 280 285
Tyr Arg Ala Lys Glu Ile Pro Asp Asn Ala Leu Ala Val Ser Val Leu
290 295 300
Ser Lys Asn Arg Ala Ile Gln Glu Asn His Lys Glu Leu Met His Ile
305 310 315 320
Lys Asn Glu Ile Leu Pro Arg Phe Lys Lys Ala Met Asp Glu Glu Lys
325 330 335
Glu Ile Cys Gly Ile Glu Asp Lys Val Ile His Pro Lys Val Met Met
340 345 350
Lys Phe Lys Ile Pro Arg Ala Gln Gln Pro Gln Ile His Val Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Ile Ser His
370 375 380
His His Ala Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Ser Ile Asp
385 390 395 400
Leu Val His Tyr Glu Asp Leu Thr Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Thr Ala Ala Gly Arg Thr Leu Thr Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ala Ile Ser Asn Ala Phe Gly Thr Gln Met His Thr Arg
435 440 445
Arg Leu Val Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Leu
450 455 460
His Tyr Asp Ile Ser Phe Ser Asp Leu Arg Gly Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Ile His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Cys Lys Val
500 505 510
Leu Gly Asp Arg Leu Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 6
<211> 552
<212> PRT
<213> 人工序列
<220>
<223> BTV1 ACR58463的NS1
<400> 6
Met Glu Arg Phe Leu Arg Lys Tyr Asn Ile Ser Gly Asp Tyr Ala Asn
1 5 10 15
Ala Thr Arg Thr Phe Leu Ala Ile Ser Pro Gln Trp Thr Cys Ser His
20 25 30
Leu Lys Arg Asn Cys Leu Phe Asn Gly Met Cys Val Lys Gln Asn Phe
35 40 45
Glu Arg Ala Met Ile Ala Ala Thr Asp Val Glu Glu Pro Ala Lys Ala
50 55 60
Tyr Lys Leu Val Glu Leu Ala Lys Glu Ala Met Tyr Asp Arg Glu Thr
65 70 75 80
Val Trp Leu Gln Cys Phe Lys Ser Phe Ser Gln Pro Tyr Glu Glu Asp
85 90 95
Val Glu Gly Lys Met Lys Arg Cys Gly Val Gln Leu Leu Glu Asp Tyr
100 105 110
Arg Lys Ser Gly Met Met Asp Glu Ala Val Lys Gln Ser Ala Leu Val
115 120 125
Asn Ser Glu Arg Val Arg Leu Asp Asp Ser Leu Ser Ala Met Pro Tyr
130 135 140
Ile Tyr Val Pro Ile Lys Glu Gly Gln Ile Val Asn Pro Thr Phe Ile
145 150 155 160
Ser Arg Tyr Arg Gln Ile Ala Tyr Tyr Phe Tyr Asn Pro Asp Ala Ala
165 170 175
Asp Asp Trp Ile Asp Pro Asn Leu Phe Gly Ile Arg Gly Gln His Asn
180 185 190
Gln Ile Lys Arg Glu Val Glu Arg Gln Ile Asn Thr Cys Pro Tyr Thr
195 200 205
Gly Tyr Lys Gly Arg Val Phe Gln Val Met Phe Leu Pro Ile Gln Leu
210 215 220
Ile Asn Phe Leu Arg Met Asp Asp Phe Ala Lys His Phe Asn Arg Tyr
225 230 235 240
Ala Ser Met Ala Ile Gln Gln Tyr Leu Arg Val Gly Tyr Ala Glu Glu
245 250 255
Val Arg Tyr Val Gln Gln Leu Phe Gly Arg Val Pro Thr Gly Glu Phe
260 265 270
Pro Leu His Gln Met Met Leu Ile Arg Arg Asp Phe Pro Thr Arg Asp
275 280 285
Arg Ser Ile Val Glu Ala Arg Val Arg Arg Ser Gly Asp Glu Asn Trp
290 295 300
Gln Ser Trp Leu Leu Pro Met Ile Ile Val Arg Glu Gly Leu Asp His
305 310 315 320
Pro Asp Arg Trp Glu Trp Leu Ile Asp Tyr Met Asp Arg Lys His Thr
325 330 335
Cys Gln Leu Cys Tyr Leu Lys His Ser Lys Gln Ile Pro Thr Cys Gly
340 345 350
Val Ile Asp Val Arg Ala Ser Glu Leu Thr Gly Cys Ser Pro Phe Lys
355 360 365
Thr Val Lys Ile Glu Glu His Val Gly Asn Asp Ser Val Phe Lys Thr
370 375 380
Lys Leu Val Arg Asp Glu Gln Ile Gly Arg Ile Gly Asp His Tyr Tyr
385 390 395 400
Thr Thr Asn Cys Tyr Thr Gly Ala Glu Ala Leu Ile Thr Thr Ala Ile
405 410 415
His Ile His Arg Trp Ile Arg Gly Cys Gly Ile Trp Asn Asp Glu Gly
420 425 430
Trp Gln Glu Gly Ile Phe Met Leu Gly Arg Val Leu Leu Arg Trp Glu
435 440 445
Leu Thr Lys Ala Gln Arg Ser Ala Leu Leu Arg Leu Phe Cys Phe Val
450 455 460
Cys Tyr Gly Tyr Ala Pro Arg Ala Asp Gly Thr Ile Pro Asp Trp Asn
465 470 475 480
Asn Leu Gly Ser Phe Leu Asp Ile Ile Leu Lys Gly Pro Glu Leu Ser
485 490 495
Glu Asp Glu Asp Glu Arg Ala Tyr Ala Thr Met Phe Glu Met Val Arg
500 505 510
Cys Ile Ile Thr Leu Cys Tyr Ala Glu Lys Val His Phe Ala Gly Phe
515 520 525
Ala Ala Pro Ala Cys Glu Ser Gly Glu Val Ile Asn Leu Ala Ala Arg
530 535 540
Met Ser Gln Met Trp Met Glu Tyr
545 550
<210> 7
<211> 349
<212> PRT
<213> 人工序列
<220>
<223> BTV1 ACR58464的VP7
<400> 7
Met Asp Thr Ile Ala Ala Arg Ala Leu Thr Val Met Arg Ala Cys Ala
1 5 10 15
Thr Leu Gln Glu Ala Arg Ile Val Leu Glu Ala Asn Val Met Glu Ile
20 25 30
Leu Gly Ile Ala Ile Asn Arg Tyr Asn Gly Leu Thr Leu Arg Gly Val
35 40 45
Thr Met Arg Pro Thr Ser Leu Ala Gln Arg Asn Glu Met Phe Phe Met
50 55 60
Cys Leu Asp Met Met Leu Ser Ala Ala Gly Ile Asn Val Gly Pro Ile
65 70 75 80
Ser Pro Asp Tyr Thr Gln His Met Ala Thr Ile Gly Val Leu Ala Thr
85 90 95
Pro Glu Ile Pro Phe Thr Thr Glu Ala Ala Asn Glu Ile Ala Arg Val
100 105 110
Thr Gly Glu Thr Ser Thr Trp Gly Pro Ala Arg Gln Pro Tyr Gly Phe
115 120 125
Phe Leu Glu Thr Glu Glu Thr Phe Gln Pro Gly Arg Trp Phe Met Arg
130 135 140
Ala Ala Gln Ala Val Thr Ala Val Val Cys Gly Pro Asp Met Ile Gln
145 150 155 160
Val Ser Leu Asn Ala Gly Ala Arg Gly Asp Val Gln Gln Ile Phe Gln
165 170 175
Gly Arg Asn Asp Pro Met Met Ile Tyr Leu Val Trp Arg Arg Ile Glu
180 185 190
Asn Phe Ala Met Ala Gln Gly Asn Ser Gln Gln Thr Gln Ala Gly Val
195 200 205
Thr Val Ser Val Gly Gly Val Asp Met Arg Ala Gly Arg Ile Ile Ala
210 215 220
Trp Asp Gly Gln Ala Ala Leu His Val His Asn Pro Thr Gln Gln Asn
225 230 235 240
Ala Met Val Gln Ile Gln Val Val Phe Tyr Ile Ser Met Asp Lys Thr
245 250 255
Leu Asn Gln Tyr Pro Ala Leu Thr Ala Glu Ile Phe Asn Val Tyr Ser
260 265 270
Phe Arg Asp His Thr Trp His Gly Leu Arg Thr Ala Ile Leu Asn Arg
275 280 285
Thr Thr Leu Pro Asn Met Leu Pro Pro Ile Phe Pro Pro Asn Asp Arg
290 295 300
Asp Ser Ile Leu Thr Leu Leu Leu Leu Ser Thr Leu Ala Asp Val Tyr
305 310 315 320
Thr Val Leu Arg Pro Glu Phe Ala Ile His Gly Val Asn Pro Met Pro
325 330 335
Gly Pro Leu Thr Arg Ala Ile Ala Arg Ala Ala Tyr Val
340 345
<210> 8
<211> 354
<212> PRT
<213> 人工序列
<220>
<223> BTV1 ACR58465的NS2
<400> 8
Met Glu Gln Lys Gln Arg Arg Phe Thr Lys Asn Ile Phe Val Leu Asp
1 5 10 15
Ala Asn Gly Lys Thr Leu Cys Gly Ala Ile Ala Lys Leu Ser Ser Gln
20 25 30
Pro Tyr Cys Gln Ile Lys Ile Gly Arg Val Ile Ala Phe Lys Pro Val
35 40 45
Lys Asn Pro Glu Pro Lys Gly Tyr Val Leu Asn Val Pro Gly Pro Gly
50 55 60
Ala Tyr Arg Ile Gln Asp Gly Gln Asp Ile Ile Ser Leu Met Leu Thr
65 70 75 80
Pro His Gly Val Glu Ala Thr Thr Glu Arg Trp Glu Glu Trp Lys Phe
85 90 95
Glu Gly Val Ser Val Thr Pro Met Ala Thr Arg Val Gln His Asn Gly
100 105 110
Val Met Val Asp Ala Glu Ile Lys Tyr Cys Lys Gly Met Gly Ile Val
115 120 125
Gln Pro Tyr Met Arg Asn Asp Phe Asp Arg Asn Glu Met Pro Asp Leu
130 135 140
Pro Gly Val Met Arg Ser Asn Tyr Asp Val Arg Glu Leu Arg Gln Lys
145 150 155 160
Ile Lys Asn Glu Arg Glu Ser Ala Pro Arg Leu Gln Val Gln Ser Val
165 170 175
Ala Pro Arg Glu Glu Ser Arg Trp Met Asp Asp Asp Glu Ala Lys Val
180 185 190
Asp Glu Glu Ala Lys Glu Met Ile Pro Gly Thr Ser Arg Leu Glu Lys
195 200 205
Leu Arg Glu Ala Arg Ser Asn Val Phe Lys Glu Val Glu Ala Gly Ile
210 215 220
Asn Trp Asn Leu Asp Glu Lys Asp Glu Glu Asp Arg Asp Glu Arg Glu
225 230 235 240
Asp Glu Glu Gln Val Lys Thr Leu Ser Asp Asp Asp Glu Gln Gly Glu
245 250 255
Asp Ala Ser Asp Asp Glu His Pro Lys Thr His Ile Thr Lys Glu Tyr
260 265 270
Ile Glu Lys Val Ala Lys Gln Ile Lys Leu Lys Asp Glu Arg Phe Met
275 280 285
Ser Leu Ser Ser Ala Met Pro Gln Ala Ser Gly Gly Phe Asp Arg Met
290 295 300
Ile Val Thr Lys Lys Leu Lys Trp Gln Asn Val Pro Leu Tyr Cys Phe
305 310 315 320
Asp Glu Ser Ser Lys Arg Tyr Glu Leu Gln Cys Val Gly Ala Cys Glu
325 330 335
Arg Val Ala Phe Val Ser Lys Asp Met Ser Leu Ile Ile Leu Pro Val
340 345 350
Gly Val
<210> 9
<211> 329
<212> PRT
<213> 人工序列
<220>
<223> BTV1 AEU03964的VP6/NS4
<400> 9
Met Ser Ala Ala Met Leu Leu Ala Pro Gly Asp Val Ile Lys Arg Ser
1 5 10 15
Ser Glu Glu Leu Lys Gln Arg Gln Ile Gln Ile Asn Leu Ile Asp Trp
20 25 30
Thr Glu Gly Glu Ser Glu Lys Glu Ser Lys Ala Glu Ala Lys Glu Gly
35 40 45
Asp Lys Ala Glu Glu Leu Lys Asp Gly Glu Gly Thr Gln Ser Glu Ser
50 55 60
Ser Gln Lys Lys Glu Ser Ser Lys Glu Thr Lys Asp Ala Asp Val Asp
65 70 75 80
Arg Arg Ile His Thr Ala Val Gly Ser Gly Ser Ser Ala Lys Gly Pro
85 90 95
Gly Glu Arg Ala Asn Glu Asn Val Asp Arg Gly Asp Gly Lys Val Gly
100 105 110
Gly Gly Gly Gly Asp Ala Asp Ala Gly Val Gly Thr Thr Gly Ala Asn
115 120 125
Gly Gly Arg Trp Val Val Leu Thr Glu Glu Ile Ala Arg Ala Ile Glu
130 135 140
Ser Lys Tyr Gly Thr Lys Ile Asp Val Tyr Arg Asp Glu Val Pro Ala
145 150 155 160
Gln Ile Ile Glu Val Glu Arg Ser Leu Gln Lys Glu Leu Gly Ile Ser
165 170 175
Arg Glu Gly Val Ala Glu Gln Thr Glu Arg Leu Arg Asp Leu Arg Arg
180 185 190
Lys Glu Lys Ser Gly Ala His Ala Lys Ala Ala Glu Arg Gly Arg Arg
195 200 205
Lys Gln Gly Lys Lys Pro His Gly Asp Ala Gln Arg Glu Gly Thr Glu
210 215 220
Glu Glu Lys Thr Ser Glu Glu Pro Ala Ser Val Gly Ile Thr Ile Glu
225 230 235 240
Gly Val Met Ser Gln Lys Lys Leu Leu Ser Met Ile Gly Gly Val Glu
245 250 255
Arg Lys Met Ala Pro Ile Gly Ala Arg Glu Ser Ala Val Met Leu Val
260 265 270
Ser Asn Ser Ile Lys Asp Val Val Arg Ala Thr Ala Tyr Phe Thr Ala
275 280 285
Pro Thr Gly Asp Pro His Trp Lys Glu Val Ala Arg Glu Ala Ser Lys
290 295 300
Lys Lys Asn Ile Leu Ala Tyr Thr Ser Thr Gly Gly Asp Val Lys Thr
305 310 315 320
Glu Phe Leu His Leu Ile Asp His Leu
325
<210> 10
<211> 229
<212> PRT
<213> 人工序列
<220>
<223> BTV1 ACR58467的NS3
<400> 10
Met Leu Ser Gly Leu Ile Gln Arg Phe Glu Glu Glu Lys Met Lys His
1 5 10 15
Asn Gln Glu Arg Val Glu Glu Leu Ser Leu Val Arg Val Asp Asp Thr
20 25 30
Ile Ser Gln Pro Pro Arg Tyr Ala Pro Ser Ala Pro Met Pro Ser Ser
35 40 45
Met Pro Thr Val Ala Leu Glu Ile Leu Asp Lys Ala Met Ser Asn Thr
50 55 60
Thr Gly Ala Thr Gln Thr Gln Lys Ala Glu Lys Ala Ala Phe Ala Ser
65 70 75 80
Tyr Ala Glu Ala Phe Arg Asp Asp Val Arg Leu Arg Gln Ile Lys Arg
85 90 95
His Val Asn Glu Gln Ile Leu Pro Lys Leu Lys Ser Asp Leu Gly Gly
100 105 110
Leu Lys Lys Lys Arg Ala Ile Ile His Met Thr Leu Leu Ile Ala Ala
115 120 125
Val Val Ala Leu Leu Thr Ser Val Cys Thr Leu Ser Ser Asp Met Ser
130 135 140
Val Ala Phe Lys Leu Asn Gly Thr Ser Ala Glu Ile Pro Gln Trp Phe
145 150 155 160
Lys Ser Leu Asn Pro Met Leu Gly Val Val Asn Leu Gly Ala Thr Phe
165 170 175
Leu Met Met Val Cys Ala Lys Ser Glu Arg Ser Leu Asn Gln Gln Ile
180 185 190
Asp Met Ile Lys Lys Glu Val Met Lys Lys Gln Ser Tyr Asn Asp Ala
195 200 205
Val Arg Met Ser Phe Thr Glu Phe Ser Ser Val Pro Leu Asp Gly Phe
210 215 220
Glu Leu Pro Leu Thr
225
<210> 11
<211> 961
<212> PRT
<213> 人工序列
<220>
<223> BTV8的VP2
<400> 11
Met Glu Glu Leu Ala Ile Pro Ile Tyr Thr Asn Val Phe Pro Ala Glu
1 5 10 15
Leu Leu Asp Gly Tyr Asp Tyr Ile Ile Asp Val Ser Ser Arg Val Glu
20 25 30
Glu Glu Gly Asp Glu Pro Val Lys Arg His Asp Val Thr Glu Ile Pro
35 40 45
Arg Asn Ser Met Phe Asp Ile Lys Asp Glu His Ile Arg Asp Ala Ile
50 55 60
Ile Tyr Lys Pro Val Asn Asn Asp Gly Tyr Val Leu Pro Arg Val Leu
65 70 75 80
Asp Ile Thr Leu Lys Ala Phe Asp Asp Arg Lys Arg Val Val Leu Asn
85 90 95
Asp Gly His Ser Glu Phe His Thr Lys Thr Asn Trp Val Gln Trp Met
100 105 110
Ile Asp Asp Ala Met Asp Val Gln Pro Leu Lys Val Asp Ile Ala His
115 120 125
Thr Arg Ser Arg Ile Ser His Ala Leu Phe Asn Cys Thr Val Arg Leu
130 135 140
His Ser Lys Lys Ala Asp Thr Ala Ser Tyr His Val Glu Pro Val Glu
145 150 155 160
Ile Glu Ser Trp Gly Cys Asn His Thr Trp Leu Ser Arg Ile His His
165 170 175
Leu Val Asn Val Glu Leu Phe His Cys Ser Gln Glu Ala Ala Tyr Thr
180 185 190
Leu Lys Pro Thr Tyr Lys Ile Ile Ser Asn Ala Glu Arg Ala Ser Thr
195 200 205
Ser Asp Ser Phe Asn Gly Thr Met Ile Glu Leu Gly Arg Asn His Gln
210 215 220
Ile Gln Met Gly Asp Gln Gly Tyr Gln Lys Leu Lys Glu Gly Leu Val
225 230 235 240
Gln Val Arg Ile Glu Gly Lys Thr Pro Leu Val Ile Gln Glu Glu Ile
245 250 255
Thr Ala Leu Asn Lys Ile Arg Glu Gln Trp Ile Ala Arg Asn Phe Asp
260 265 270
Gln Arg Glu Ile Lys Val Leu Asp Leu Cys Arg Leu Leu Ser Thr Ile
275 280 285
Gly Arg Lys Met Cys Asn Thr Glu Glu Glu Pro Lys Asn Glu Ala Asp
290 295 300
Leu Ser Val Lys Phe Gln Met Glu Leu Asp Glu Ile Phe Arg Pro Gly
305 310 315 320
Asn Asn Glu Arg Thr Asn Ile Met Gly Gly Gly Val His Arg Lys Asn
325 330 335
Glu Asp Arg Phe Tyr Val Leu Ile Met Ile Ala Ala Ser Asp Thr Asn
340 345 350
Lys Gly Arg Ile Trp Trp Ser Asn Pro Tyr Pro Cys Leu Arg Gly Ala
355 360 365
Leu Ile Ala Ala Glu Val Gln Leu Gly Asp Val Tyr Asn Leu Leu Arg
370 375 380
Asn Trp Phe Gln Trp Ser Val Arg Pro Thr Tyr Val Pro Tyr Asp Arg
385 390 395 400
Asn Arg Glu Ser Asp Lys Tyr Ile Tyr Ser Arg Ile Asn Leu Phe Asp
405 410 415
Ser Thr Leu Arg Pro Gly Asp Lys Ile Val His Trp Glu Tyr Lys Leu
420 425 430
Leu Asn Glu Val Arg Glu Val Ser Ile Asn Lys Gly Asn Glu Cys Asp
435 440 445
Leu Phe Pro Glu Asp Glu Glu Phe Thr Thr Lys Phe His Glu Ala Arg
450 455 460
Tyr Thr Glu Met Lys Asn Gln Ile Ile Gln Ser Gly Trp Asn Gln Arg
465 470 475 480
Asp Phe Lys Met His Lys Ile Leu Glu Asp Gly Ala Asn Val Leu Thr
485 490 495
Ile Asp Phe Glu Lys Asp Ala His Ile Gly Thr Gly Ser Ala Leu Ser
500 505 510
Leu Pro Asp Tyr Tyr Asn Lys Trp Ile Ile Ala Pro Met Phe Asn Ala
515 520 525
Lys Leu Arg Ile Thr Glu Val Val Ile Gly Thr Ala His Thr Asp Asp
530 535 540
Pro Ala Val Gly Arg Ser Ala Lys Ala Phe Thr His Asp Pro Phe Asp
545 550 555 560
Leu Gln Arg Tyr Cys Leu Ala Arg Tyr Tyr Asp Val Arg Pro Gly Met
565 570 575
Met Gly Arg Ala Leu Ser Lys Gln Gln Asn Met Ser Ser Met Thr Asp
580 585 590
Lys Leu Ser Lys Gln Glu Asp Tyr Ala Gly Ile Val Ser Arg Arg Leu
595 600 605
Glu Tyr Lys Glu Arg Glu Asn Arg Cys Leu Thr Glu Thr Ala Gln Tyr
610 615 620
Val Phe Glu Lys Thr Cys Leu Tyr Val Leu Glu Leu Leu Ser Arg His
625 630 635 640
Thr Met Pro Ser Glu Asp Ser Glu Val Thr Phe Glu His Pro Thr Ile
645 650 655
Asp Pro Ser Val Asp Ile Glu Thr Trp Lys Ile Ile Asp Val Ser Gln
660 665 670
Leu Ile Ile Phe Val Phe Asp Tyr Leu Phe Glu Asn Arg Lys Ile Val
675 680 685
Arg Asp Thr Thr Glu Ala Arg Trp Thr Leu Phe Lys Ile Arg Ser Glu
690 695 700
Val Gly Arg Ala Arg Ile Asp Ala Ile Glu Met Thr Phe Pro Arg Phe
705 710 715 720
Gly Arg Met Leu Arg Asn Ala Ser Gln Ala Lys Ile Asn Gln Asp Ile
725 730 735
Ala Cys Leu Asn Phe Leu Pro Leu Leu Phe Ile Ile Gly Asp Asn Ile
740 745 750
Ser Tyr Ala His Arg Gln Trp Ser Ile Pro Val Leu Leu Tyr Ala His
755 760 765
Asp Ile Arg Ile Ile Pro Leu Glu Val Gly Ala Tyr Asn Asn Arg Phe
770 775 780
Gly Leu Thr Ser Tyr Leu Glu Tyr Met Ala Phe Phe Pro Ser Tyr Ala
785 790 795 800
Thr Arg Val Ala Lys Ile Asp Glu Ser Ile Lys Glu Cys Ala Ile Ala
805 810 815
Met Ala Glu Phe Tyr Met Asn Thr Asp Ile His Ser Gly Ser Val Met
820 825 830
Ser Asn Val Ile Thr Thr Lys Arg Leu Leu Tyr Glu Thr Tyr Leu Ala
835 840 845
Ser Leu Cys Gly Gly Tyr Ser Asp Gly Leu Leu Trp Tyr Leu Pro Ile
850 855 860
Thr His Pro Ser Lys Cys Leu Val Ala Phe Glu Val Ala Asp Asp Val
865 870 875 880
Val Pro Leu Ser Val Arg Arg Glu Arg Ile Leu Ser Arg Phe Pro Leu
885 890 895
Ser Ser Arg His Val Lys Gly Ile Ala Leu Ile Ser Val Asp Arg Asn
900 905 910
Gln Lys Val Ser Val Gln Thr Glu Gly Ile Val Thr His Arg Leu Cys
915 920 925
Lys Lys Asn Leu Leu Lys Tyr Val Cys Asp Val Ile Leu Phe Lys Phe
930 935 940
Ser Gly His Val Phe Gly Asn Asp Glu Met Leu Thr Lys Leu Leu Asn
945 950 955 960
Val
<210> 12
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV8 CAM57247的VP5
<400> 12
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Leu Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Met Gly Ser
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Met Gln
85 90 95
Thr Lys Ile Arg Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Ile Arg
100 105 110
Leu Lys Tyr Asn Asp Lys Ile Lys Gln Lys Phe Gly Lys Glu Leu Glu
115 120 125
Glu Val Tyr Glu Phe Met Asn Gly Val Ala Lys Gln Glu Glu Asp Glu
130 135 140
Glu Lys His Tyr Asp Val Leu Lys Lys Ala Val Asn Ser Tyr Asp Lys
145 150 155 160
Ile Leu Thr Glu Glu Glu Lys Gln Met Arg Ile Leu Ala Thr Ala Leu
165 170 175
Gln Lys Glu Val Lys Glu Arg Thr Gly Thr Glu Ala Val Met Val Lys
180 185 190
Glu Tyr Arg Asn Lys Ile Asp Ala Leu Lys Glu Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Thr His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Thr Ile Val Ser Thr Val Leu Asp
275 280 285
His Lys Phe Lys Asp Ile Pro Asp Glu Met Leu Ala Val Ser Val Leu
290 295 300
Ser Lys Asn Arg Ala Ile Glu Glu Asn His Lys Glu Ile Ile His Leu
305 310 315 320
Lys Asn Glu Ile Leu Pro Arg Phe Lys Lys Ala Met Asp Glu Glu Lys
325 330 335
Glu Ile Cys Gly Ile Glu Asp Lys Lys Ile His Pro Lys Val Met Met
340 345 350
Lys Phe Lys Ile Pro Arg Thr Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Ile Ser His
370 375 380
His His Ala Asn Glu Ser Phe Phe Ile Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Leu Val His Tyr Glu Asp Leu Thr Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Ala Ala Val Gly Arg Ser Leu Asn Glu Val Tyr Lys Glu Phe
420 425 430
Leu Asn Leu Ala Ile Asn Asn Thr Tyr Ser Ser Gln Met His Ala Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Leu
450 455 460
His Tyr Asp Ile Ser Phe Ser Thr Leu Arg Ser Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Glu Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Ile Lys His Gly Val Lys Ile
500 505 510
Leu Gly Thr Glu Val Asp Ile Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 13
<211> 962
<212> PRT
<213> 人工序列
<220>
<223> BTV2 AEO19855的VP2
<400> 13
Met Asp Glu Leu Gly Ile Pro Ile Tyr Gly Arg Asn Tyr Pro Glu His
1 5 10 15
Leu Leu Lys Gly Tyr Glu Phe Leu Ile Asn Thr Gly Val Arg Tyr Pro
20 25 30
Ser Gln Gly Gly Arg His Asp Val Ser Lys Ile Pro Glu Met Phe Ala
35 40 45
Tyr Asp Ile Lys Asp Glu Gly Ile Arg Glu Ala Leu Lys Tyr Met Pro
50 55 60
Thr Arg Asn Asp Gly Val Val Leu Pro Arg Ile Val Asp Ile Ser Leu
65 70 75 80
Lys Gly Tyr Asp Ile Arg Lys Ser Val Ile Glu Ala Asn Lys Ser Asn
85 90 95
Ser Phe His Thr Asp Thr Asn Trp Val Gln Trp Met Ile Lys Asp Ser
100 105 110
Met Asp Gln Gln Pro Leu Lys Ile Ser Ile Asp Glu Glu His Ser Arg
115 120 125
Val Val His Ser Leu Phe Asn Cys Gln Val Lys Ile Asp Ala Lys Lys
130 135 140
Ala Asp Thr Leu Ser Tyr His Leu Glu Ala Ile Glu Asp Ala Glu Lys
145 150 155 160
Ala Cys Leu His Thr Arg Gly Gln Leu Cys Asn His Leu Ala Arg Met
165 170 175
Asp Leu Leu His Ala Ala Gln Glu Ile Ala Tyr Ala Ile Lys Pro Thr
180 185 190
Tyr Gln Leu Ile Val His Ser Glu Arg Ala Ser Thr Ser Asp Asn Phe
195 200 205
Glu Leu Gly Arg Gln Asp Val Ile Thr Leu Arg Arg Gly His Arg Ile
210 215 220
Gln Met Gly Asp Glu Ala Tyr Thr Lys Leu Met Glu Arg Leu Val Arg
225 230 235 240
Leu Thr Val Gln Gly Asn Val Pro Arg Lys Ile Gln Ser Glu Ile Glu
245 250 255
Gln Leu Glu Ala Ile Arg Thr Arg Trp Ala Thr Gly Arg Tyr Asp Pro
260 265 270
Ala His Ile Asn Ser Gln Asp Leu Cys Arg Ile Leu Ser Arg Ile Gly
275 280 285
Arg Ile Met Leu Asp Gln Glu Ala Glu Pro Val Asp Glu Asp Ser Leu
290 295 300
Ser Leu Arg Phe Gln Arg Ala Leu Asp Glu Lys Phe Arg Leu Asn Asp
305 310 315 320
Ser Glu Arg Asn Lys Ile Phe Glu Pro Lys Ser His Arg Lys Asp Glu
325 330 335
Asp Arg Phe Tyr Val Leu Leu Ala Ile Ala Ala Ser Asp Thr Tyr Asn
340 345 350
Ser Arg Ile Trp Trp Ser Asn Pro Tyr Pro Cys Leu Arg Gly Thr Leu
355 360 365
Ile Ala Ala Glu Thr Lys Leu Gly Asp Val Tyr Phe Thr Leu Arg Ser
370 375 380
Trp Tyr Asp Trp Ser Val Arg Ser Ser Tyr Val Pro Arg Glu Arg Glu
385 390 395 400
Arg Glu Thr Glu Lys Tyr Ile Phe Ser Lys Ile Asn Leu Phe Asp Tyr
405 410 415
Glu Ala Gly Pro Ser Ser Lys Val Ile His Trp Glu Tyr Gln Leu Tyr
420 425 430
Lys Arg Glu Arg Val Val Thr Leu Glu Arg Gly Asn Pro Cys Asp Leu
435 440 445
Tyr Pro Asp Glu Asp Asp Glu Val Ile Ile Thr Lys Phe Asp Asp Ala
450 455 460
Lys Tyr Ser Glu Met Val Gly Glu Ile Ile Asp Gly Gly Trp Asn Asp
465 470 475 480
Glu Glu Phe Lys Met Tyr Lys Leu Leu Gln Glu Lys Gly Asn Val Leu
485 490 495
Thr Ile Asp Phe Glu Lys Asp Thr Lys Leu Asn Ser Thr Ser Glu Val
500 505 510
Val Leu Pro Asp Tyr Tyr Gly Lys Trp Ile Val Ala Pro Met Phe Asn
515 520 525
Ser Lys Met Arg Ile Ile Glu Thr Glu Ile Ala Thr Asn Arg Ser Asp
530 535 540
Asp Pro Met Ile Lys Arg Thr Leu Lys Pro Met Thr Asp Asp Pro Val
545 550 555 560
Glu Leu Gln Arg Tyr Thr Leu Ala Arg Tyr Tyr Asp Ile Arg Pro Gly
565 570 575
Leu Met Gly Arg Ser Leu Asn Arg Thr Gln Thr Gln Ser Thr Phe Asp
580 585 590
Ala Lys Val Ser Glu Leu Ser Asp Tyr Glu Lys Val Val Ser Arg Phe
595 600 605
Gly Val Ile Lys Lys Pro Thr Arg Pro Cys Val Thr Leu Thr Gly Arg
610 615 620
Tyr Ile Leu Glu Lys Tyr Ser Leu Leu Leu Ile Asp Ile Leu Lys Tyr
625 630 635 640
His Thr Glu Val Glu Glu Asn Pro Gln Glu Glu Phe Thr His Pro Arg
645 650 655
Ile Asp Ser Gln Phe Lys Phe Asn Gly Asn Thr Leu Ser Asp Leu Asn
660 665 670
Gln Thr Val Val Phe Ile Val Asp Tyr Leu His Glu Lys Arg Asn Tyr
675 680 685
Val Arg Ser Val Tyr Glu Ala Arg Tyr Ile Ile Ser Arg Ile Arg Ser
690 695 700
Ser Thr Gly Ala Ala Arg Met Ser Ile Leu Glu Phe Tyr Phe Pro Thr
705 710 715 720
Phe Ala Arg Leu Ile Ser Asn Ala Arg Glu Pro Thr Tyr Val Lys Asp
725 730 735
Leu Met Ala Leu Asn Phe Leu Pro Leu Leu Phe Ile Val Gly Asp Asn
740 745 750
Met Ile Tyr Lys His Arg Gln Trp Ser Ile Pro Leu Leu Leu Tyr Thr
755 760 765
Asp Arg Val Lys Val Ile Pro Leu Glu Val Gly Ser Ser Asn Asn Arg
770 775 780
Gln Gly Leu Val Ser Tyr Leu Glu Tyr Met Phe Phe Phe Pro Ser Leu
785 790 795 800
Ala Asp Arg Thr Ser Lys Val Asp Glu Ser Met Ile Lys Val Ser Lys
805 810 815
Glu Val Val Asn Tyr Tyr Met Lys Thr Thr Ile Ser Glu Gly Gly Val
820 825 830
Asn Leu Asn Val Val Ser Thr Lys Ser Leu Leu Tyr Asp Ile Tyr Leu
835 840 845
Ser Ser Val Cys Gly Gly Val Ser Asp Gly Val Val Trp Tyr Leu Pro
850 855 860
Ile Thr His Pro Tyr Lys Cys Val Val Ala Ile Glu Val Cys Asp Asp
865 870 875 880
Arg Val Pro Ala Arg Leu Arg Cys Asp Arg Leu Arg Leu Arg Phe Pro
885 890 895
Leu Ser Ala Gln His Leu Lys Gly Ile Val Val Ile Gln Ile Asn Glu
900 905 910
Glu Gly Gly Phe Asp Val Tyr Thr Glu Gly Ile Val Thr His Arg Val
915 920 925
Cys Lys Lys Ser Leu Leu Lys His Val Cys Asp Ile Val Leu Leu Lys
930 935 940
Phe His Gly His Val Phe Gly Asn Asp Glu Met Leu Thr Lys Leu Leu
945 950 955 960
Asn Val
<210> 14
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV2 AEO19851的VP5
<400> 14
Met Gly Lys Val Ile Arg Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Ser Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Leu Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ser
65 70 75 80
Gly Glu Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Gln Gly Ile Gln
85 90 95
Thr Lys Leu Lys Glu Leu Glu Glu Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Asp Lys Ile Lys Glu Lys Phe Gly Glu Glu Leu Glu
115 120 125
Gln Val Tyr Glu Phe Met Ser Gly Ala Ala Lys Glu Glu Ala Gly Asp
130 135 140
Glu Lys Gln Phe Asp Ile Leu Ser Lys Ala Val Asn Ser Tyr Asn Lys
145 150 155 160
Ile Leu Thr Glu Glu Asp Leu Gln Met Arg Arg Leu Ala Ile Ala Leu
165 170 175
Gln Lys Glu Ile Gly Glu Arg Thr His Ala Glu Thr Thr Met Val Lys
180 185 190
Glu Tyr Arg Asn Lys Ile Asp Ala Leu Lys Ser Ala Ile Glu Ile Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Thr His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Ser Val Val Ser Thr Ile Leu Glu
275 280 285
Tyr Arg Ala Lys Asp Ile Pro Asp Asn Ala Leu Ala Val Ser Val Leu
290 295 300
Ser Lys Asn Arg Ala Ile Gln Glu Asn His Lys Glu Leu Met His Ile
305 310 315 320
Lys Asp Glu Ile Leu Pro Arg Phe Lys Lys Ala Met Asp Glu Glu Lys
325 330 335
Glu Ile Cys Gly Ile Glu Asp Lys Val Ile His Pro Lys Val Met Met
340 345 350
Lys Phe Lys Ile Pro Arg Ser Gln Gln Pro Gln Ile His Ile Tyr Thr
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Ile Ser His
370 375 380
His His Ala Asn Glu Ser Phe Phe Ile Gly Phe Asp Leu Ser Ile Asp
385 390 395 400
Leu Val His Tyr Glu Asp Leu Thr Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Met Ala Val Gly Arg Thr Leu Thr Glu Ala Tyr Lys Glu Phe
420 425 430
Leu Asn Leu Ala Ile Ser Ser Thr Tyr Gly Thr Gln Met His Thr Arg
435 440 445
Arg Leu Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Leu
450 455 460
His Tyr Asp Ile Ser Phe Ala Asp Leu Arg Gly Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Cys Lys Ile
500 505 510
Leu Gly Asp Arg Leu Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 15
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV2 南非 CAE53012的VP5
<400> 15
Met Gly Lys Val Ile Arg Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Ser Ala Leu Thr Ser Asn Ala Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Leu Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ser
65 70 75 80
Gly Glu Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Gln Gly Met Gln
85 90 95
Thr Lys Leu Lys Glu Leu Glu Glu Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Asp Lys Ile Lys Glu Lys Phe Gly Glu Glu Leu Glu
115 120 125
Gln Val Tyr Glu Phe Met Asn Gly Ala Ala Lys Glu Glu Val Gly Asp
130 135 140
Glu Lys Gln Phe Asp Ile Leu Ser Lys Ala Val Asn Ser Tyr Asn Lys
145 150 155 160
Ile Leu Thr Glu Glu Asp Gln Gln Met Arg Arg Leu Ala Ile Ala Leu
165 170 175
Gln Lys Glu Ile Gly Glu Arg Thr His Val Glu Thr Ala Met Val Lys
180 185 190
Glu Tyr Arg Asn Lys Ile Asp Ala Leu Lys Ser Ala Ile Glu Ile Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Thr His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Ser Val Val Ser Thr Ile Leu Glu
275 280 285
Tyr Arg Ala Lys Asp Ile Pro Asp Asn Ala Leu Ala Val Ser Val Leu
290 295 300
Ser Lys Asn Arg Ala Ile Gln Glu Asn His Lys Glu Leu Met His Ile
305 310 315 320
Lys Asp Glu Ile Leu Pro Arg Phe Lys Lys Ala Met Asp Glu Glu Lys
325 330 335
Glu Ile Cys Gly Ile Glu Asp Lys Thr Ile His Pro Lys Val Met Met
340 345 350
Lys Phe Lys Ile Pro Arg Ser Gln Gln Pro Gln Ile His Ile Tyr Thr
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Ile Ser His
370 375 380
His His Ala Asn Glu Ser Phe Phe Ile Gly Phe Asp Leu Ser Ile Asp
385 390 395 400
Leu Val His Tyr Glu Asp Leu Thr Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Met Ala Val Gly Arg Thr Leu Thr Glu Ala Tyr Lys Glu Phe
420 425 430
Leu Lys Leu Ala Ile Ser Ser Thr Tyr Gly Thr Gln Met His Thr Arg
435 440 445
Arg Leu Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Leu
450 455 460
His Tyr Asp Ile Ser Phe Ala Asp Leu Arg Gly Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Cys Lys Ile
500 505 510
Leu Gly Asp Arg Leu Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 16
<211> 959
<212> PRT
<213> 人工序列
<220>
<223> BTV3 CAE51090的VP2
<400> 16
Met Glu Glu Leu Val Ile Pro Val Ile Ser Arg Gln Phe Asp Lys Lys
1 5 10 15
Leu Val Gly Arg Tyr Glu Tyr Val Ile Glu Leu Ala Glu Pro Glu Gln
20 25 30
Asp Glu Trp Thr Asn His Asp Val Thr Gln Ile Pro Gly Arg Arg Met
35 40 45
Phe Asp Val Ala Gln Gln Gly Ile Arg Glu Ala Ile Ile Tyr Lys Pro
50 55 60
Leu Asp Asn Asp Gly Glu Val Leu Pro Arg Ile Leu Asp Met Ser Ile
65 70 75 80
Ala Cys Tyr Asp Met Arg Lys Thr Met Met Lys Lys Glu Gly Val Asp
85 90 95
Phe Val Ser Asn Thr Arg Trp Leu Glu Trp Met Ile Gln Asp Ser Met
100 105 110
Asp Val Gln Pro Leu Arg Val Gln Met Lys Glu Asp His Ser Thr Val
115 120 125
Gln Tyr Asp Met Phe Ser Ala Lys Val His Ile Asp Ser Arg Lys Ala
130 135 140
Asp Thr Thr Ser Tyr His Ala Ile Ala Val Glu Thr Lys Ala Glu Arg
145 150 155 160
Lys Cys Cys His Val Arg Thr Glu Val Trp Asn Ser Val Val Arg Asn
165 170 175
His Leu Phe Asn Thr Ala Gln Glu Ser Cys Tyr Thr Phe Lys Gln Thr
180 185 190
Tyr Glu Leu Ile Val Asn Ser Glu Arg Leu Ser Thr Glu Glu Glu Phe
195 200 205
Arg Val Gly Ala Pro Gln Phe His Thr Ile Gln Arg Asn His Arg Met
210 215 220
Gln Leu Gly Asp Asn Ala Tyr Asp Lys Phe Leu Lys Gly Leu Val Gln
225 230 235 240
Leu Arg Val Ser Gly Thr Thr Pro Ala Lys Ile Arg Asp Glu Val Ala
245 250 255
Ala Leu Asp Val Ile Arg Asp Asn Trp Ile Arg Gly Ser Phe Asp Arg
260 265 270
Ser His Ile Lys Ser Leu Glu Leu Cys Arg Leu Leu Ser Ser Ile Gly
275 280 285
Arg Lys Met Val Asn Met Glu Glu Glu Pro Lys Asp Glu Lys Asp Leu
290 295 300
Ser Val Lys Phe Gln Phe Arg Leu Asp Glu Lys Phe Ser Pro Asn Asp
305 310 315 320
Pro Glu Arg Asn Val Ile Phe Thr Ser Lys Thr His Arg Thr Asn Glu
325 330 335
Asp Arg Phe Tyr Val Leu Leu Val Ile Ala Ala Ser Asp Thr Asn Asn
340 345 350
Gly Arg Val Trp Trp Ser Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu
355 360 365
Ile Ala Ala Glu Cys Lys Leu Gly Asp Val Tyr His Thr Leu Arg Ser
370 375 380
Lys Tyr Glu Trp Gly Val Arg Pro Thr Tyr Lys Pro Lys Asp Leu Glu
385 390 395 400
Arg Glu Arg Glu Lys Tyr Val Val Gly Arg Val Asn Leu Phe Asp Leu
405 410 415
Glu Gly Glu Pro Ala Thr Lys Val Ile His Trp Glu Tyr Glu Leu Ile
420 425 430
Ser Pro Thr Tyr Ser Val Ser Asn His Lys Gly Asn His Cys Asp Leu
435 440 445
Tyr Pro Asp Asp Val Glu Ile Thr Thr Lys Phe Asn Glu Asp Arg Tyr
450 455 460
Arg Glu Met Ile Gln Ser Val Ile Asp Asp Gly Trp Asp Gln Lys Asn
465 470 475 480
Leu Lys Met Tyr Lys Ile Leu Glu Glu Glu Gly Asn Pro Leu Leu Tyr
485 490 495
Asp Leu Glu Lys Asp Ile Lys Leu Asp Ser Gln Ser Gln Val Val Phe
500 505 510
Pro Ser Tyr Tyr Asn Lys Trp Thr His Ala Pro Met Phe Asn Ala Arg
515 520 525
Val Lys Pro Cys Asp Ile Glu Leu Ala Glu Arg Lys Asn Asp Asp Pro
530 535 540
Phe Val Lys Arg Thr Leu Lys Pro Ile Lys Ala Asp Cys Val Asp Leu
545 550 555 560
Leu Arg Tyr His Met Ser His Tyr Tyr Asp Leu Arg Pro Ser Leu Lys
565 570 575
Gly Val Ser Leu Ser Asn Lys Gln Thr Pro Ser Gly Ile His Gln Ala
580 585 590
Leu Val Gln Asp Asp Leu Tyr Ser Arg Leu Leu Arg Arg Arg Asp Val
595 600 605
Asp Leu Asp Tyr Ser Ser Pro Cys Pro Ile Ile Thr Asn Tyr Phe Leu
610 615 620
Leu Glu Lys Phe His Ile Leu Ile Leu Thr Ile Met Glu Lys His Tyr
625 630 635 640
Trp Glu Leu Asp Asp Ser Asp Asp Val Tyr Glu Phe Pro Lys Ile Asp
645 650 655
Ala Ser Ala Phe Glu Val Asp Gly Thr Leu Tyr Asp Ile Ser Gln Thr
660 665 670
Ile Val His Met Tyr Asp Arg Phe Phe Glu Lys Arg Arg Val Leu Arg
675 680 685
Ser Ile Asp Glu Ser Arg Trp Ile Leu His Leu Ile Arg Ile Ser Gln
690 695 700
Gly Arg Glu Arg Leu Glu Val Ile Glu Arg Phe Phe Pro Asn Tyr Gly
705 710 715 720
Lys Ala Met Arg Gln Arg Asp Phe Lys Lys Val Arg Asp Val Met Phe
725 730 735
Leu Asn Phe Leu Pro Phe Phe Phe Leu Thr Gly Asp Asn Ile Ser Tyr
740 745 750
Glu His Arg Gln Trp Ser Ile Pro Ile Ile Leu Tyr Ala Asp Lys Leu
755 760 765
Arg Ile Leu Pro Ile Glu Val Gly Ala His Tyr Asn Arg Phe Gly Val
770 775 780
Thr Cys Ile Leu Glu Leu Leu Asn Phe Phe Pro Ser Tyr Glu Lys Arg
785 790 795 800
Glu Glu Lys Leu Glu Glu Asp Ile Val Leu Cys Ala Asp Ala Ile Val
805 810 815
Asn Phe Tyr Leu Gln Thr Thr Ile Ser Asn Gly Gly Val Gln Thr Ser
820 825 830
Ile Val Ser Thr Lys Ala Leu Leu Tyr Glu Met Tyr Leu Ser Ser Ile
835 840 845
Cys Gly Gly Tyr Ser Glu Gly Val Leu Trp Tyr Leu Pro Ile Thr His
850 855 860
Pro Val Lys Cys Leu Val Ala Leu Glu Val Ser Asp Ala Leu Val Gly
865 870 875 880
Ala Asp Val Arg Ile Asp Lys Ile Arg Arg Arg Phe Pro Leu Ser Ala
885 890 895
Lys His Leu Lys Gly Ile Val Gln Ile Ser Val His Pro Asn Arg Thr
900 905 910
Phe Ser Val Thr Thr Cys Gly Ile Val Lys His Lys Val Cys Lys Lys
915 920 925
Thr Leu Leu Lys His Arg Cys Asp Val Ile Leu Leu Gln Thr Pro Gly
930 935 940
Tyr Val Phe Gly Asn Asp Glu Leu Leu Thr Lys Leu Leu Asn Ile
945 950 955
<210> 17
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV3 CAE53013的VP5
<400> 17
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ala
65 70 75 80
Gly Asp Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Met Lys Ile Lys Glu Ile Glu Glu Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys His Gly Lys Glu Ile Ala Lys Lys Phe Gly Asp Glu Leu Glu
115 120 125
Glu Ile Tyr Gln Phe Met Asn Gly Glu Val Lys Glu Glu Val Gln Gln
130 135 140
Glu Glu Gln Tyr Lys Val Leu Cys Lys Ala Val Asn Ser Tyr Glu Lys
145 150 155 160
Leu Leu Leu Ala Glu Asn Asp Glu Met Arg Thr Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Thr Glu Arg Thr Glu Thr Glu Ser Lys Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Ala Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Val Gly Ser
225 230 235 240
Gly Met Ala Thr Ala Ile Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Thr Met Val Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Arg Asp Ile Pro Asp Lys Glu Leu Ala Val Ser Ile Leu
290 295 300
Ala Lys Asn Asn Ala Ile Val Ala Asn Thr Arg Glu Val Gln His Ile
305 310 315 320
Lys Glu Glu Ile Leu Pro Lys Phe Lys Lys Ile Met Asp Glu Glu Lys
325 330 335
Glu Leu Glu Gly Ile Asp Asp Lys Lys Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Val Pro Arg Pro Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
Phe His Val Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Ala Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Glu Ala Lys Gly Arg Thr Leu Asn Glu Ala Tyr Lys Glu Phe
420 425 430
Leu Asn Leu Ser Ile Gly Ser Ala Tyr Thr Ser Pro Met His Ala Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Thr Leu Lys Ser Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Val
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Lys Asn Ala
515 520 525
<210> 18
<211> 956
<212> PRT
<213> 人工序列
<220>
<223> BTV4 ABB71699的VP2
<400> 18
Met Glu Glu Phe Val Ile Pro Val Phe Ser Glu Arg Glu Ile Pro Tyr
1 5 10 15
Ala Leu Ile Asn Arg Tyr Pro Leu Ala Ile Gln Thr Asp Val Lys Ile
20 25 30
Ala Asp Val Glu Gly Tyr His Asn Leu Ala Lys Ile Pro Glu Ser Asp
35 40 45
Met Val Asp Val Pro Lys Leu Asp Ile Val Ser Ala Leu Asn Tyr Lys
50 55 60
Pro Thr Arg Asn Asp Gly Ile Val Val Pro Arg Leu Leu Asp Ile Thr
65 70 75 80
Leu Lys Ala Tyr Asp Asp Arg Lys Ser Val Lys Asn Ala Arg Gly Val
85 90 95
Asp Phe Met Thr Asp Ala Lys Trp Met Lys Trp Ala Ile Asp Asp Arg
100 105 110
Met Asp Ile Gln Pro Leu Lys Ile Thr Leu Asp Glu His Tyr Ala Val
115 120 125
Asn His Gln Leu Phe Asn Cys Ile Val Lys Ala Lys Thr Ala Asn Ala
130 135 140
Asp Thr Ile Tyr Tyr Asp Tyr Phe Pro Leu Glu Asp Arg Ala Lys Lys
145 150 155 160
Cys Asn His Thr Asn Leu Glu Leu Leu Arg Ser Leu Thr Thr Ile Glu
165 170 175
Ala Phe His Val Leu Gln Gly Ala Ala Tyr Ser Leu Lys Ser Asn Tyr
180 185 190
Asp Leu Ile Ala Asn Ser Glu Arg Glu Ser Leu Glu Glu Ser Tyr Pro
195 200 205
Ile Gly Ser Glu Lys Trp Val His Leu Ile Arg Arg Thr Lys Ile Gly
210 215 220
Asn Ser Gly Leu Ser Tyr Asn Arg Phe Ile Ser Ser Met Val Gln Val
225 230 235 240
Val Val Arg Gly Lys Val Pro Asp Ile Ile Arg Gly Glu Ile Thr Gln
245 250 255
Leu Asn Arg Ile Arg Thr Glu Trp Ile Ser Ala Ser Tyr Asp Arg Thr
260 265 270
Arg Ile Arg Ala Leu Glu Leu Cys Asn Ile Leu Ser Ala Ile Gly Arg
275 280 285
Lys Met Met Asp Ala His Glu Glu Pro Lys Asp Glu Met Asp Leu Ser
290 295 300
Thr Arg Phe Gln Phe Lys Leu Asp Glu Lys Phe Asn Thr Ser Asp Ser
305 310 315 320
Glu His Val Asn Ile Phe Arg Thr Ser Gly Ala Ala Thr Asn Glu Gly
325 330 335
Arg Phe Tyr Ala Leu Ile Ala Ile Ala Ala Thr Asp Thr Gln Lys Gly
340 345 350
Arg Val Trp Arg Thr Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu Ile
355 360 365
Ala Ser Glu Cys Glu Leu Gly Asp Val Tyr Tyr Thr Leu Arg His Val
370 375 380
Tyr Arg Trp Ser Leu Arg Pro Glu Tyr Gly Gln Arg Glu Arg Gln Leu
385 390 395 400
Glu Asp Asn Lys Tyr Val Phe Gly Arg Val Asn Leu Phe Asp Ser Asp
405 410 415
Leu Ala Val Gly Asp Gln Val Ile His Trp Arg Tyr Glu Ile Thr Glu
420 425 430
Pro Val Lys Thr Thr Tyr Asp Asn Gly Tyr Ile Cys Asn Pro Glu Glu
435 440 445
Lys Asp Asp Glu Leu Leu Cys Lys Ile Asp Asp Glu Arg Tyr Lys Glu
450 455 460
Met Met Glu Arg Leu Ile Glu Gly Gly Trp Asp Gln Glu Arg Phe Lys
465 470 475 480
Leu His Asn Ile Leu Thr Glu Pro Asn Leu Leu Thr Ile Asp Phe Glu
485 490 495
Lys Asp Ala Tyr Leu Asn Ser Arg Ser Glu Leu Val Phe Pro Asn Tyr
500 505 510
Phe Asp Lys Trp Ile Asn Ser Pro Met Phe Asn Ala Arg Leu Arg Ile
515 520 525
Thr His Gly Glu Ile Gly Ser Ser Lys Thr Ile Asp Pro Trp Asn Arg
530 535 540
Arg Val Val Tyr Gly Tyr Ile Lys Thr Ser Ile Glu Ser Leu Asp Tyr
545 550 555 560
Thr Leu Gly Lys Tyr Tyr Asp Ile Arg Leu Gln Leu Phe Gly Asp Thr
565 570 575
Leu Ser Gln Lys Gln Thr Gln Ser Ala Val Phe Thr Tyr Leu Ser Gly
580 585 590
Gln Asp Asp Phe Pro Ala Leu Thr Asn Tyr Leu Lys Gly Glu Ala Val
595 600 605
Cys Pro His Ala Gly Gly Ala Val Tyr Thr Phe Arg Lys Met Ala Leu
610 615 620
Ser Leu Ile Ala Ser Tyr Glu Lys Leu Ser Pro Glu Met His Glu Gly
625 630 635 640
Leu Glu His Gln Thr Tyr Val His Pro Ser Val Asn Thr Ala Tyr Gln
645 650 655
Lys Gln Val Lys Asp Met Lys Asp Leu Ser Gln Leu Ile Cys Phe Ile
660 665 670
Ile Asp Cys Ile Phe Glu Lys Arg Val Gln Ile Arg Gly Val Gly Glu
675 680 685
Ala Arg Arg Ile Val Tyr Leu Ile Gln Asn Ser Thr Gly Leu Gln Arg
690 695 700
Gln Glu Val Leu Lys Arg Thr Phe Pro Asn Phe Leu Met Arg Leu Phe
705 710 715 720
Lys Leu Arg Glu Val Lys Arg Ile Cys Asp Leu Asn Val Ile Asn Phe
725 730 735
Leu Pro Leu Leu Phe Leu Val Gln Asp Asn Ile Ser Tyr Trp His Arg
740 745 750
Gln Trp Ser Val Pro Met Ile Leu Phe Asp Asp Ala Val Arg Leu Ile
755 760 765
Pro Val Glu Val Gly Ala Tyr Ala Asn Arg Phe Gly Phe Lys Ser Phe
770 775 780
Tyr Asn Phe Ile Arg Phe His Pro Gly Asp Ser Lys Lys Lys Gln Asp
785 790 795 800
Ala Asp Asp Met His Lys Glu Tyr Gly Val Ala Cys Phe Glu Tyr Tyr
805 810 815
Met Asn Thr Lys Ile Ser Gln Gly Gly Val Asn Val Pro Val Val Thr
820 825 830
Ser Lys Leu Asp Thr Leu Lys Ile His Leu Ala Ser Leu Cys Ser Gly
835 840 845
Leu Ala Asp Ser Ile Val Tyr Thr Leu Pro Val Ala His Pro Lys Lys
850 855 860
Cys Ile Val Leu Ile Val Val Gly Asp Asp Lys Leu Asp Pro Gln Val
865 870 875 880
Arg Ser Glu Gln Val Leu Ser Lys Tyr Tyr Tyr Ser Arg Arg His Ile
885 890 895
Cys Gly Val Val Ala Val Ser Val Gly Gln Glu Gly Gln Leu Gln Val
900 905 910
Tyr Ser Thr Gly Ile Val Arg His Arg Ile Cys Glu Lys Ser Ile Leu
915 920 925
Lys Tyr Lys Cys Lys Val Val Leu Val Arg Met Pro Gly His Val Phe
930 935 940
Gly Asn Asp Glu Leu Met Thr Lys Leu Leu Asn Val
945 950 955
<210> 19
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV4 CAE53015的VP5
<400> 19
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Ser Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ala Ala Thr Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Thr
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Met Gln
85 90 95
Thr Lys Ile Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Lys Glu Ile Thr Lys Glu Phe Gly Lys Glu Leu Glu
115 120 125
Glu Val Tyr Asp Phe Met Asn Gly Glu Ala Lys Glu Glu Glu Val Val
130 135 140
Gln Glu Gln Tyr Ser Met Leu Cys Lys Ala Val Asp Ser Tyr Glu Lys
145 150 155 160
Ile Leu Lys Ala Glu Asp Ser Lys Met Ala Ile Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Ser Glu Arg Ser Gln Asp Glu Ile Lys Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Asp Ala Ile Glu Ile Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Met Arg Ser Pro Lys Ile Glu Pro Thr Ile Ile Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Lys Glu Ile Pro Asp Glu Gln Leu Ala Val Ser Val Leu
290 295 300
Asn Lys Lys Thr Ala Val Thr Asp Asn Cys Asn Glu Ile Ala His Ile
305 310 315 320
Lys Gln Glu Ile Leu Pro Lys Phe Lys Gln Ile Met Asp Glu Glu Lys
325 330 335
Glu Ile Glu Gly Ile Glu Asp Lys Val Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Ile Pro Arg Thr Gln Gln Pro Gln Ile His Ile Tyr Ala
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Arg Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Thr Ser His Trp His Ala Leu Gly Leu
405 410 415
Ala Gln Glu Ala Ser Gly Arg Thr Leu Thr Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ser Ile Ser Asn Thr Tyr Ser Ser Ala Ile His Ala Arg
435 440 445
Arg Met Ile Arg Ser Arg Ala Val His Pro Ile Phe Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Ala Leu Lys Asn Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Glu Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Ile
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 20
<211> 955
<212> PRT
<213> 人工序列
<220>
<223> BTV5 CAE51092的VP2
<400> 20
Met Asp Glu Phe Gly Val Pro Ile Phe Arg Ser Ser Gln Val Pro His
1 5 10 15
His Ile Leu Glu Gly Tyr Asp Ile Lys Val Asp Leu Ser Asp Arg Val
20 25 30
Val Gln Glu Asp Gly Arg His Asp Pro Asn Lys Leu Lys Glu Arg Lys
35 40 45
Thr Ile Asp Val Pro Asn Gly Glu Ala Arg Ser Asp Leu Asn Tyr Asn
50 55 60
Pro Thr Asp Asn Glu Gly Asn Gln Leu Pro Arg Ala Leu Asp Ile Ala
65 70 75 80
Leu Cys Ala Arg Leu Thr Arg Lys Ser Phe Asn Thr Asn Ser Gly Val
85 90 95
Thr Phe His Thr Asp His Lys Trp Met Glu Trp Met Leu Ser Asp Ala
100 105 110
Met Asp Val Gln Pro Leu Arg Ile Asp Leu Ala Ala Gln Asn Glu Tyr
115 120 125
Ala Lys Cys Asp Leu Phe Asn Ser Ser Val Tyr Val Arg Lys Lys Tyr
130 135 140
Val Asp Ala Ile Ser Tyr Arg Tyr Ile Ser Val Glu Asp Asp Ser Lys
145 150 155 160
Gly Cys Asn His Thr Arg Val Tyr Ser Val Asn His Met Ile Asn Cys
165 170 175
Gly Leu Tyr Asn Val Ala Gln Glu Cys Ala Tyr Ala Phe Lys His Thr
180 185 190
Asn Thr Leu Ile Val Asn Ser Gln Arg Glu Thr Thr Ser Glu Pro Phe
195 200 205
Gln Pro Gly Asn Pro Lys Ile Gly Ser Leu Gly Arg Arg Ala Arg Val
210 215 220
Asp Met Ala Asp Pro Gly Tyr Pro Leu Phe Arg Ala Gly Leu Leu Gln
225 230 235 240
Ile Ile Val Asn Gly Ala Ile Pro Ala Asp Ile Arg Ser Glu Met Asp
245 250 255
Arg Leu Asn Gln Ile Arg Glu Ala Trp Lys Arg Asp Lys Asn Thr Arg
260 265 270
Glu Val Arg Ala Leu Asp Leu Cys Val Leu Leu Ser Lys Ile Gly Arg
275 280 285
Ile Lys Met Asn Met Glu Glu Glu Pro Lys Asp Glu Gly Val Met Ser
290 295 300
Leu Arg Phe Gln Gly Lys Ile Asp Ala Met Phe Phe Ser Glu Asn Thr
305 310 315 320
Glu Lys Thr Asn Ile Met Arg Asp Gly Ser Gly Arg Thr Asp Asp Glu
325 330 335
Arg Phe Tyr Ala Leu Leu Leu Ile Cys Ala Thr Asp Ala Phe Ser Arg
340 345 350
Arg Ile Trp Arg Thr Asn Pro Tyr Pro Cys Leu Arg Gly Thr Leu Ile
355 360 365
Ala Ala Glu Cys Val Leu Gly Asp Pro Tyr Lys Thr Leu Arg Arg Lys
370 375 380
Phe Asp Trp Ser Val Arg His Ala Gly Asp Lys Met Leu Glu Gly Ser
385 390 395 400
Ser Tyr Val Phe Thr Arg Ile Asn Leu Phe Asp Thr Asp Lys Ala Pro
405 410 415
Gly Ser Arg Val Ile His Trp Ile Gln Glu Thr Leu Pro Val Ile Lys
420 425 430
Thr Thr Trp Ala Glu Gly Cys Ser Leu Ala Asp Glu Ala Ser Asp Asp
435 440 445
His Leu His Cys Lys Ile Asp His Ala Gln Tyr Arg Asp Leu Val Ser
450 455 460
Arg Val Ile Asn Gly Gly Trp Asp Gln Glu Asn Phe Lys Ile His Lys
465 470 475 480
Leu Phe Val Glu Arg Gly Asn Ile Phe Leu Met Asp Phe Glu Lys Asp
485 490 495
Ala Lys Ile Thr Thr Gln Ser Glu Val Glu Tyr Pro Tyr Tyr Tyr Asp
500 505 510
Thr Trp Ile Tyr Ala Pro Ile Phe Ser Thr Arg Tyr Lys Ile Thr Glu
515 520 525
Thr Glu Ile Ala Asn Ala Lys Ser Leu Asp Pro Ala Ile Lys Arg Thr
530 535 540
Leu Ala Pro Leu Ser Asp Asp Pro Val Ala Leu Gln Leu Arg Ala Val
545 550 555 560
Gly His Leu Tyr Asp Thr Arg Pro Ala Ile Met Gly Gln Thr Leu Ser
565 570 575
Pro Arg Gln Asn Gln Thr Ala Leu His Lys Thr Leu Leu Asp Asp Pro
580 585 590
Glu Asn Asp Arg Tyr Tyr Glu Ala Arg Ser Ile Lys Lys Val Asp Asn
595 600 605
Pro Cys Ser Val His Tyr Thr Ser Ser Phe Ile Leu Gln Lys Ile Ser
610 615 620
Glu Leu Val Leu Ser Leu Met Ile Tyr His Ile Glu His Asp Trp Glu
625 630 635 640
Pro Leu Lys Gln Tyr Thr His Pro Glu Ile Ser Thr Glu Phe Gly Glu
645 650 655
Ile Ser Cys Glu Ile Cys Asp Ile Ser Gln Leu Thr Val Leu Leu Ile
660 665 670
Asp Leu Phe Phe Glu Asn Arg Arg Thr Val Arg Pro Ser Glu Glu Thr
675 680 685
Arg Tyr Ile Ile His Gln Ile Arg Glu Ser Arg Gly Asp Gly Arg Leu
690 695 700
Lys Met Leu Glu Arg Phe Phe Pro Ala Tyr Gly Gln Ala Leu Arg Lys
705 710 715 720
Ile Glu Ser Ala Thr Glu Val Gly His Ile Thr Met Leu Asn Phe Leu
725 730 735
Pro Phe Phe Phe Leu Leu Gly Asp Asn Ile Ile Tyr Lys His Arg Glu
740 745 750
Trp Thr Val Pro Ile Leu Ile Tyr Thr Asp Gly Val Met Ile Trp Pro
755 760 765
Ala Gln Val Gly Ala Asn Tyr Asn Arg Phe Gly Phe Cys Gly Phe Leu
770 775 780
Asn Tyr Met Arg Phe His Ala Gly Ser Gln Leu Arg Ser Lys Val Leu
785 790 795 800
Asp Asp Ala Glu Lys Thr Ile Ala Lys Glu Met Leu Lys Tyr Tyr Met
805 810 815
Lys Thr Asp Ile Phe Ser Gly Gly Val Gln Lys Asn Ile Arg Val Thr
820 825 830
Lys Ser Met Gln Met Glu Ile Tyr His Ser Ser Leu Cys Gly Gly Leu
835 840 845
Ser Asp Ala Val Val Tyr Ala Leu Pro Ile Ser Phe Pro Ile Lys Cys
850 855 860
Leu Thr Leu Ile Ile Ile Gly Asp Asp Leu Val Glu Pro Gln Leu Arg
865 870 875 880
Ile Ser Arg Val Met Asp Tyr Phe Lys His Val Ser Glu His Val Arg
885 890 895
Gly Val Ala Ser Ile Ser Ile Ser Arg Asn Gly Asp Val Ser Thr Tyr
900 905 910
Ser Arg Gly Ile Val His Val Glu Leu Leu Lys Lys Asn Ile Leu Lys
915 920 925
Tyr Gln Phe Gln Val Ala Leu Leu Lys Val Lys Gly Tyr Val Phe Gly
930 935 940
Asn Asp Glu Met Leu Ile Lys Leu Leu Asn Val
945 950 955
<210> 21
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV5 CAE53016的VP5
<400> 21
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ala
65 70 75 80
Gly Asp Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Met Lys Ile Lys Glu Ile Glu Glu Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys His Gly Lys Glu Ile Thr Arg Lys Phe Gly Ala Glu Leu Glu
115 120 125
Glu Ile Tyr Gln Phe Met Asn Gly Glu Met Arg Glu Glu Ala Glu Gln
130 135 140
Glu Glu Gln Tyr Lys Val Leu Cys Lys Ala Val Asn Ser Tyr Glu Lys
145 150 155 160
Leu Leu Val Ala Glu Asn Asp Gln Met Arg Ala Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Thr Glu Arg Thr Glu Ala Glu Ser Thr Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Ala Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Val Gly Ser
225 230 235 240
Gly Met Ala Thr Ala Ile Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Thr Met Val Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Asp Glu Ile Pro Asp Lys Glu Leu Ala Val Ser Ile Leu
290 295 300
Ala Lys Asn Asn Ala Ile Val Ala Asn Thr Arg Glu Val Gln His Ile
305 310 315 320
Lys Asp Glu Ile Leu Pro Lys Phe Lys Lys Ile Met Asp Glu Glu Lys
325 330 335
Glu Leu Glu Gly Ile Asp Asp Lys Lys Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Val Pro Arg Ser Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
Phe His Ala Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Ala Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Glu Ala Lys Gly Arg Thr Leu Ser Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ser Ile Gly Ser Ala Tyr Thr Ser Pro Met His Ala Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Thr Leu Lys Ser Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Val
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 22
<211> 955
<212> PRT
<213> 人工序列
<220>
<223> BTV6 CAE51093的Vp2
<400> 22
Met Asp Glu Leu Ala Ile Pro Ile Ile Asn Gly His Phe Pro Lys Glu
1 5 10 15
Ala Leu Ile Glu Tyr Asp Cys Ile Val Glu Leu Asn Ala Pro Ser Glu
20 25 30
Asp Asp Glu Val Asp Val Thr Gln Ile Pro Thr Lys Asn Met Met Asp
35 40 45
Ile Pro Asp Val Pro Ile Arg Glu Ala Leu Arg Val Leu Pro Gln Arg
50 55 60
Asn Asp Gly His Val Leu Thr Arg Ile Leu Asp Ile Ser Leu Ser Gly
65 70 75 80
Tyr Asp Arg Arg Lys Tyr Tyr Lys Thr Asn Lys Gly Gly Glu Phe Glu
85 90 95
Ser Pro Lys Glu Trp Leu Asn Trp Met Ile His Asp Ser Met Asp Val
100 105 110
Gln Pro Leu Lys Val Ser Leu Glu Met Arg Pro Thr Val Lys His Glu
115 120 125
Leu Phe Ser Ser Asn Val Tyr Leu Ser Val Lys Lys Ala Asp Thr Ser
130 135 140
Ser Tyr His Ile Glu Ala Ile Gly Asp Thr Gly Lys Ala Cys Asp His
145 150 155 160
Ser Tyr Val Ser Met Trp Asn Asn Leu Val Arg His Glu Val Met His
165 170 175
Cys Ala Gln Glu Ser Ala Tyr Val Leu Lys Pro Thr Met Glu Ile Ile
180 185 190
Val His Ser Glu Arg Ala Ser Thr Asp Val Pro Phe Gln Leu Val Gly
195 200 205
Gln Asn Tyr Val Ser Ile Gly Arg Asn His Arg Leu Val Leu Gly Asp
210 215 220
Glu Pro Tyr Lys Lys Thr Leu Gln Gly Leu Thr Lys Leu Arg Val Gln
225 230 235 240
Gly Lys Val Pro Pro Gln Ile Gln Asp Glu Ile Gln Gln Leu Asn Arg
245 250 255
Ile Arg Asp Ala Trp Ile Gly Asp Ala Tyr Asn Pro Arg His Ile Arg
260 265 270
Ser Leu Glu Leu Cys Arg Ile Leu Ser Ser Val Gly Arg Lys Val Val
275 280 285
Cys Ile Glu Glu Glu Pro Lys Asn Glu Ser Asp Ile Ser Val Lys Phe
290 295 300
Gln Phe Lys Leu Asp Glu Lys Phe Arg Leu Asn Asp Gly Glu Arg Gly
305 310 315 320
Val Ile Phe Thr Ser Lys Asn Gln Arg Asn Asp Gln Asp Arg Phe Phe
325 330 335
Val Leu Ile Met Ile Ala Ala Ser Asp Thr Tyr Asn Gly Arg Ile Trp
340 345 350
Trp Thr Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu Ile Ala Ser Glu
355 360 365
Thr Arg Leu Gly Asp Val Tyr Trp Thr Leu Arg His Thr Tyr Asp Trp
370 375 380
Ser Val Arg Met Thr Tyr Ser Pro Arg Met Lys Glu Arg Glu Asp Asp
385 390 395 400
Lys Tyr Ile Tyr Gly Arg Val Asn Leu Phe Asp Leu Asp Ala Ser Pro
405 410 415
Gly Asp Lys Val Val His Trp Arg Tyr Glu Leu Ile Ser Gln Thr Lys
420 425 430
Lys Thr Thr Phe His Asp Gly Asn Pro Cys Asp Leu Phe Pro Asp Val
435 440 445
Glu Gly Ile Val Thr Lys Phe Asn Lys Thr Ala Tyr Ser Asn Met Ile
450 455 460
Asn Glu Leu Leu Asn Gly Gly Trp Asn Thr Arg Glu Phe Lys Met Tyr
465 470 475 480
Lys Leu Leu Glu Asp Glu Gly Asn Val Leu Thr Ile Asp Leu Glu Lys
485 490 495
Asp Thr Lys Leu Asn Ser Arg Ser Glu Phe Ile Leu Pro Asn Tyr Tyr
500 505 510
Asp Lys Trp Ile Tyr Ala Pro Met Phe Asn Ala Lys Leu Lys Ile Thr
515 520 525
Glu Thr Glu Ile Ala Gln Ala Lys Asn Asp Asp Pro Tyr Val Ile Arg
530 535 540
Thr Leu Ala Pro Ile Gly Ser Asn Pro Phe Thr Leu Gln Arg Leu Cys
545 550 555 560
Leu Gly Asn Tyr Tyr Asp Val Arg Pro Ala Met His Gly Arg Ala Leu
565 570 575
Ser Lys Lys Gln Glu Gln Ser Ala Tyr His Ala Ala Leu Val Lys Glu
580 585 590
Lys Asp Tyr Glu Asp Ile Leu Thr Arg Arg Gly Leu Arg Lys Ala Asp
595 600 605
Lys Pro Cys Pro Leu Thr Thr Cys Ala Tyr Ile Leu Glu Lys Thr Ala
610 615 620
Leu Leu Trp Ile Asp Val Met Glu Lys His Ile Ser Glu Val Thr Asp
625 630 635 640
Asp Ser Asn Glu Phe Asp Phe Pro Lys Ala Cys Val Asp Ser Pro Val
645 650 655
Gly Thr Asp Arg Ile Val Asp Val Thr Gln Leu Ile Val Leu Leu Ile
660 665 670
Asp Trp Ile Tyr Glu Arg Arg Lys Asp Val Arg Ser Val Asp Glu Ser
675 680 685
Arg Trp Val Ile Glu Thr Ile Arg Arg Ala Arg Gly Arg Asn Arg Leu
690 695 700
Glu Leu Ile Ser Arg Glu Phe Pro Asn Phe Gly Arg Ala Val Ala Arg
705 710 715 720
Leu Gln Ser Pro Asn Thr Val Gly Asp Val Met Val Ile Asn Phe Leu
725 730 735
Pro Phe Leu Phe Leu Met Gly Asp Asn Val Ser Tyr Glu His Arg Gln
740 745 750
Trp Ser Val Pro Val Ile Leu His Ala Asp Val Met Trp Phe Ile Pro
755 760 765
Val Glu Val Gly Ala Phe Tyr Asn Arg Phe Gly Val Thr Ser Phe Leu
770 775 780
Glu Tyr Leu Met Phe Phe Pro Ser Phe His Met Arg Thr Val Arg Leu
785 790 795 800
Asp Glu Ala Glu Val Lys Val Ser Lys Leu Ile Leu Asp Phe Tyr Leu
805 810 815
Asp Thr Thr Ile Phe Ser Gly Gly Ile Gln Ser Ser Val Val Ser Thr
820 825 830
Lys Gln Ile Leu Tyr Glu Thr Tyr Leu Ser Ser Leu Cys Gly Gly Phe
835 840 845
Ser Glu Gly Leu Val Trp Tyr Leu Pro Ile Thr His Pro Thr Lys Cys
850 855 860
Val Val Ala Ile Glu Phe Ala Asp Asn Lys Val Ser Ala Val Tyr Arg
865 870 875 880
Cys Asn Arg Leu Arg Ala Arg Phe Pro Leu Ser Ser Ile His Leu Arg
885 890 895
Gly Ile Ala Ile Leu Thr Ile Gly Lys Asn Arg Met Val Asp Ala Tyr
900 905 910
Thr Glu Gly Ile Val Ser His Arg Ile Cys Lys Lys Asn Val Leu Gly
915 920 925
Tyr Thr Cys Gln Ile Leu Leu Leu Lys Phe Ser Gly His Val Phe Gly
930 935 940
Asn Asp Glu Met Leu Thr Lys Leu Leu Asn Val
945 950 955
<210> 23
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV6 CAE53019的Vp5
<400> 23
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ala
65 70 75 80
Gly Asp Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Met Lys Ile Lys Glu Ile Glu Glu Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys His Gly Lys Glu Ile Ala Lys Lys Phe Gly Asp Glu Leu Glu
115 120 125
Glu Ile Tyr Gln Phe Met Asn Gly Glu Val Lys Glu Glu Val Gln Gln
130 135 140
Glu Glu Gln Tyr Lys Val Leu Cys Lys Ala Val Asn Ser Tyr Glu Lys
145 150 155 160
Leu Leu Leu Ala Glu Asn Asp Glu Met Arg Thr Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Thr Glu Arg Thr Glu Thr Glu Ser Lys Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Ala Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Val Gly Ser
225 230 235 240
Gly Met Ala Thr Ala Ile Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Thr Met Val Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Arg Asp Ile Pro Asp Lys Glu Leu Ala Val Ser Ile Leu
290 295 300
Ala Lys Asn Asn Ala Ile Val Ala Asn Thr Arg Glu Val Gln His Ile
305 310 315 320
Lys Glu Glu Ile Leu Pro Lys Phe Lys Lys Ile Met Asp Glu Glu Lys
325 330 335
Glu Leu Glu Gly Ile Asp Asp Lys Lys Ile His Pro Arg Val Met Met
340 345 350
Lys Phe Lys Val Pro Arg Ser Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
Phe His Val Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Ala Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Glu Ala Lys Gly Arg Thr Leu Asn Glu Ala Tyr Lys Glu Phe
420 425 430
Leu Asn Leu Ser Ile Gly Ser Ala Tyr Thr Ser Pro Met His Ala Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Thr Leu Lys Ser Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Val
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Lys Asn Ala
515 520 525
<210> 24
<211> 959
<212> PRT
<213> 人工序列
<220>
<223> BTV7 CAE51094的Vp2
<400> 24
Met Asp Glu Phe Ala Ile Pro Ile Val His Gly Arg Val Ile Pro Tyr
1 5 10 15
Asp Leu Val Arg Ser Tyr Asp Ile Val Val Asp Leu Asp Val Arg Ile
20 25 30
Asp Glu Glu Gly Asp Lys His Asp Thr Thr Lys Ile Pro Glu Ala Tyr
35 40 45
Met Cys Asp Val Pro Asn Ile Ala Met Gln Asp Ala Asn Ser Ala Ile
50 55 60
Leu Gly Arg Asn Asp Gly Tyr Ala Leu Pro Arg Ile Leu Asp Val Ser
65 70 75 80
Ile Ala Ala Tyr Asp Pro Arg Lys Gly Tyr Trp Lys Tyr Arg Gly Ile
85 90 95
Glu Lys Met Thr Asn Gln Thr Trp Leu Lys Trp Ala Ile Lys Asp Phe
100 105 110
Met Asp Val Gln Pro Leu Lys Val Asp Leu Ala Asp Leu Glu Gly Lys
115 120 125
Val Arg Tyr Asp Met Phe Asn Cys Ser Ala Arg Ile Asp Ala Lys Lys
130 135 140
Ala Glu Ala Ile Tyr Tyr Gly Trp Ala Ala Gln Glu Val Val Asn Lys
145 150 155 160
Gly Cys Asn His Ala Lys Ser Glu Met Phe Gln His Val Val Arg Cys
165 170 175
Glu Leu Leu Asn Cys Ser Gln Glu Ser Ala Tyr Ile Leu Lys Pro Thr
180 185 190
Gln Lys Ile Glu Pro Leu Ala Glu Lys Asn Asp Leu Gly Glu Arg Leu
195 200 205
Gly Pro Gly Ser Asn Asn Leu Ile Asn Met Arg Arg Gly Asp Thr Phe
210 215 220
Arg Met Gly Thr Asp Gln Tyr Asn Arg Met Leu Gln Gly Trp Tyr Arg
225 230 235 240
Val Lys Val Lys Gly Gln Ile Pro Asp Met Val Arg Glu Glu Met Ala
245 250 255
Glu Leu Val Lys Ile Arg Asp Arg Trp Lys Val Ala Lys Asn Pro Arg
260 265 270
Glu Ile Lys Ser Leu Glu Leu Cys Lys Leu Leu Ser Thr Ile Gly Arg
275 280 285
Arg Met Tyr Asn Met Tyr Glu Glu Pro Lys Asp Glu Glu Ala Val Ser
290 295 300
Leu Arg Phe Gln Trp Asn Leu Asp Glu Ile Phe Lys Thr Asp Asn Arg
305 310 315 320
Glu His Val Asn Ile Ala Val Asn Lys Asn Gly Arg Thr Asp Asp Glu
325 330 335
Arg Phe Phe Ala Leu Ile Met Ile Ala Ala Thr Asp Thr Asn Arg Gly
340 345 350
Arg Ile Trp Trp Ser Asn Met Phe Pro Cys Leu Arg Gly Ala Leu Ile
355 360 365
Ala Ser Glu Thr Gln Phe Gly Asp Val Tyr Lys Met Leu Arg Val Arg
370 375 380
Phe Glu Trp Ser Val Arg Asn Glu Tyr Ser Pro Gln Ala Asp Val Ala
385 390 395 400
Arg Gln Asn Glu Asn Tyr Val Tyr Ala Arg Val Asn Leu Phe Asp Leu
405 410 415
Val Ala Asp Pro Ala Thr Ser Val Ile His Trp Glu Tyr Lys Leu Asp
420 425 430
Lys Pro Phe Lys Thr Cys Tyr Ser Asp Gly His Gln Cys Asp Glu Tyr
435 440 445
Pro Asp Glu Tyr Asp Leu Ile Cys Ser Phe Asp Glu Ala Lys Tyr Ser
450 455 460
Glu Met Val Gln Arg Ile Ile Glu Gly Gly Trp Asp His Lys Asn Phe
465 470 475 480
Lys His Tyr Lys Ile Leu Lys Glu Ala Ser Asn Val Leu Thr Ile Asp
485 490 495
Phe Glu Lys Asp Ala Lys Leu Asn Glu Ser Ser Glu Met Asn Leu Pro
500 505 510
Tyr Tyr Phe Asn Lys Asp Ile Ala Ala Pro Met Phe Arg Cys Val Leu
515 520 525
Lys Val Cys Glu Thr Ser Ile Ser Glu Arg His Asn Leu Asp Pro Ala
530 535 540
Ile Ser Arg Thr Met Lys Pro Met Arg Ala Lys Ala Ala Asp Leu Leu
545 550 555 560
Asp Tyr Ser Leu Gly Ser Tyr Tyr Glu Thr Arg Ser Gly Val Met Gly
565 570 575
Arg Thr Leu Thr Thr Thr Gln Lys Gln Ser Asp Met Tyr Lys Met Val
580 585 590
Gln Asp Glu Asp Asp Tyr Leu Ser Leu Met Val Gly Arg Gly Val Thr
595 600 605
Asp Lys Phe Ala Asn Pro Cys Pro Ile Thr Thr Ala Gly Gln Tyr Ala
610 615 620
Leu Gln Lys Thr Ser Val Met Ile Phe Ser Arg Val Glu His Tyr Phe
625 630 635 640
Glu Glu Tyr Leu Lys Asp Asp Lys Val Glu Tyr Leu Ser Pro Ser Leu
645 650 655
Asn Leu Glu Trp Val Glu Ile Thr Ser Arg Ile Thr Asp Ile Ser Gln
660 665 670
Leu Ile Val Leu Thr Phe Asp Val Phe Phe Glu Gly Arg Arg Gln Val
675 680 685
Arg Thr Ile Asp Glu Ser Arg Phe Ile Leu Tyr Gln Ile Arg Ala Arg
690 695 700
Lys Gly Met Asp Arg Leu Ser Tyr Ile Lys Asp Leu Phe Pro Gln Leu
705 710 715 720
Tyr Gly Trp Trp Val Ser Pro Glu Pro Thr Tyr Gly His Ala Gln Lys
725 730 735
Leu Asn Leu Leu Pro Leu Leu Phe Ile Ile Gly Asp Asn Phe Ile Tyr
740 745 750
Glu His Arg Gln Trp Ser Tyr Pro Val Ile Leu Val Ser His Lys Leu
755 760 765
Arg Phe Val Pro Val Glu Leu Gly Ala Tyr Tyr Asn Arg Phe Gly Leu
770 775 780
Thr Ser Leu Phe Ser Tyr Leu Val Phe His Val Gly Lys Ser Lys Tyr
785 790 795 800
Gly Leu Glu Leu Asp Glu Ala Glu Lys Lys Val Leu Gln Ala Ala Lys
805 810 815
Asp Tyr Tyr Ala Val Ala Thr Ile Ser Glu Gly Gly Ile Asn Thr His
820 825 830
Ser Thr Met Thr Lys Asp Pro Ile Tyr Gln Val Tyr Leu Ser Thr Ile
835 840 845
Cys Gly Gly Leu Leu Asp Ser Leu Ile Tyr Leu Met Pro Ile Thr His
850 855 860
Pro Val Lys Ser Ile Ile Met Val Ile Ile Asn Asp Gly Arg Gly Ser
865 870 875 880
Ala Ser Asp Cys Val Arg Arg Ala Thr Arg Arg Leu Arg Tyr Ile Gln
885 890 895
Arg His Ile Arg Gly Ser Val Val Val Ser Val Leu Arg Asp Gly Val
900 905 910
Ile Asn Thr Tyr Ser Glu Gly Ile Ala Lys Ala Met Leu Cys Glu Lys
915 920 925
Ser Val Leu Lys Tyr Asn Tyr Lys Val Val Leu Val Lys Val Lys Gly
930 935 940
Tyr Val Phe Gly His Asp Glu Met Leu Thr Lys Leu Leu Asn Val
945 950 955
<210> 25
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV7 CAE53020的VP5
<400> 25
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Val Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Arg Ala Ala Glu Arg Phe Ala Glu Ser Glu Val Gly Ala Ala Thr Ile
35 40 45
Asp Gly Ile Val Gln Gly Ser Val His Ser Ile Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ser
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Thr Lys Leu Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Leu Asn Lys Lys Ile Val Asp Lys Phe Gly Thr Glu Leu Glu
115 120 125
Glu Val Tyr Asp Phe Met Asn Gly Ala Ala Lys Asp Asp Glu Asn Glu
130 135 140
Ile Lys Gln Tyr Asp Ile Leu Cys Lys Ala Val Asp Ser Tyr Gly Lys
145 150 155 160
Ile Leu Arg Glu Glu Asp Lys Lys Met Leu Glu Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ser Glu Ser Arg Ser Asp Glu Glu Thr Lys Met Val Arg
180 185 190
Glu Tyr Arg Ser Lys Ile Asp Ala Leu Arg Asp Ala Val Asp Val Glu
195 200 205
Arg Glu Ser Met Thr Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ser Asp Val Leu Glu Ala Ala Ala Glu Glu Val Pro Leu Val Gly Ser
225 230 235 240
Gly Met Ala Thr Ala Ile Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Thr His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Ser Leu Ile Ala Thr Thr Leu Glu
275 280 285
Tyr Arg Asp Arg Pro Ile Pro Asp Asp Ser Leu Ala Val Ser Val Leu
290 295 300
Glu Lys Arg Arg Ala Ile Glu Glu Asn Lys Asn Glu Ile Glu His Ile
305 310 315 320
Lys Asn Glu Ile Leu Pro Arg Phe Lys Lys Ala Met Glu Glu Glu Lys
325 330 335
Glu Ile Ser Gly Ile Glu Glu Ser Val Ile His Pro Lys Val Met Met
340 345 350
Arg Phe Lys Ile Pro Arg Ser Gln Gln Pro Gln Ile His Ile Tyr Cys
355 360 365
Ser Pro Trp Asp Ser Asp Thr Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Met Asn Glu Ser Phe Phe Ile Gly Phe Asp Leu Glu Leu Asp
385 390 395 400
Leu Val His Phe Glu Asp Leu Thr Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Met Val Val Gly Arg Thr Phe Gln Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Gly Arg Ala Met Met Asp Pro Ser Ser Gly Ala Met His Lys Arg
435 440 445
Arg Met Ser Arg Ser Arg Ser Val His Pro Ile Tyr Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Ser Tyr Ala Thr Leu Lys Ser Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Val Leu Arg Gly Pro Ile His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys His Gly Ile Lys Ile
500 505 510
Leu Asn Asp Lys Ile Asp Leu Thr Ser Leu Leu Arg Asn Ala
515 520 525
<210> 26
<211> 955
<212> PRT
<213> 人工序列
<220>
<223> BTV9 南非 AEO19747的VP2
<400> 26
Met Asp Glu Phe Gly Ile Pro Leu Tyr Arg Ser Thr His Val Pro His
1 5 10 15
His Val Leu Glu Asp Tyr Glu Leu Arg Ile Asp Leu Ser Asp Arg Val
20 25 30
Val His Gly Gly Asp Arg His Asp Pro Lys Lys Leu Lys Glu Arg Lys
35 40 45
Ala Ile Asp Val Pro Asp Gly Asp Val Arg Leu Asp Leu Asn Tyr Asn
50 55 60
Pro Thr Asp Asn Glu Gly Asn Gln Ile Pro Gly Ala Leu Asp Ile Ala
65 70 75 80
Leu Cys Ala Arg Ile Thr Arg Lys Ser Val Lys Thr Asn Glu Gly Val
85 90 95
Thr Phe Thr Thr Asn His Lys Trp Met Glu Trp Met Leu Leu Asp Ala
100 105 110
Met Asp Val Gln Pro Leu Lys Ile Asp Phe Ala Ala Leu Gly Gly Ala
115 120 125
Val Lys Cys Asp Leu Phe Asn Ser Ala Ile Tyr Val Lys Lys Lys His
130 135 140
Ala Asp Ala Ile Met Tyr Arg Tyr Ile Ala Val Glu Asp Glu Ser Arg
145 150 155 160
Gly Cys Asn His Ser Arg Val Gln Asp Val Asn His Leu Ile Asn Asn
165 170 175
Gly Leu Tyr Asn Val Ala Gln Glu Cys Ala Tyr Ala Phe Lys Asp Thr
180 185 190
Tyr Val Leu Arg Val His Ser Gln Arg Glu Asn Val Glu Glu Pro Phe
195 200 205
Glu Ile Gly Arg Pro Lys Val Gly Ser Leu Gly Arg Asn Ala Val Ile
210 215 220
Ser Met Glu Asn Pro Gly Tyr Pro Leu Phe Arg Ala Gly Leu Leu Gln
225 230 235 240
Ile Thr Val Ser Gly Thr Ile Pro Ala Asp Ile Asn Asp Glu Met Asn
245 250 255
Arg Leu Asn Gln Ile Arg Ser Ala Trp Ile Arg Asp Lys Ala Thr Arg
260 265 270
Glu Val Lys Ala Ile Glu Leu Cys Thr Leu Leu Ser Arg Ile Gly Arg
275 280 285
Leu Lys Met Asp Met Glu Glu Glu Pro Lys Asp Glu Gly Ala Met Ser
290 295 300
Leu Arg Phe Gln Gly Lys Ile Asp Thr Met Phe Phe Ser Glu Asn Thr
305 310 315 320
Glu Lys Thr Asn Ile Met Arg Asn Ile Ser Gly Arg Thr Asp Glu Glu
325 330 335
Arg Phe Tyr Val Leu Leu Leu Ile Cys Ala Thr Asp Ala Tyr Thr Arg
340 345 350
Arg Ile Trp Arg Ser Asn Pro Tyr Pro Cys Leu Arg Gly Thr Leu Ile
355 360 365
Ala Thr Glu Cys Val Leu Gly Asp Ala Tyr Lys Thr Leu Arg Gln Lys
370 375 380
Phe Asp Trp Ser Val Arg His Ala Ala Asp Lys Ala Ile Glu Asn Phe
385 390 395 400
Ser His Ile Phe Thr Arg Ile Asn Leu Phe Asp Thr Gly Lys Thr Pro
405 410 415
Gly Ala Arg Ile Ile His Trp Val Gln Glu Met Thr Met Gly Thr Lys
420 425 430
Thr Ser Trp Glu Ser Gly Tyr Pro Leu Lys Asp Glu Ala Pro Asp Asp
435 440 445
Glu Leu His Cys Arg Val Asp Arg Asp Gln His Arg Gln Met Val Thr
450 455 460
Arg Ile Ile Asp Gly Gly Trp Asp His Glu Asn Phe Lys Ile His Lys
465 470 475 480
Leu Phe His Glu Gln Gly Asn Ile Phe Leu Met Asp Phe Glu Lys Asp
485 490 495
Ala Arg Leu Thr Ser Arg Ser Glu Val Glu Tyr Pro Tyr Tyr Tyr Asp
500 505 510
Arg Trp Ile Tyr Ala Pro Ile Phe Asp Thr Arg Tyr Lys Ile Met Glu
515 520 525
Thr Glu Ile Ala Thr Ala Lys Asn Ala Asp Pro Ala Ile Lys Arg Thr
530 535 540
Leu Ala Pro Leu Met Asp Asp Pro Val Met Leu Gln Leu Arg Thr Ile
545 550 555 560
Gly Asn Leu Tyr Asp Thr Arg Pro Ala Ile Met Gly Ser Thr Leu Ser
565 570 575
Ala Arg Gln Ala Gln Thr Ser Leu His Lys Thr Leu Leu Asp Glu Ala
580 585 590
Glu Asn Gly Arg Tyr Tyr Arg Ser Arg Gly Leu Glu Lys Arg Ala Asn
595 600 605
Pro Cys Ser Val Cys Tyr Thr Ser Ser Phe Val Leu Gln Lys Val Ser
610 615 620
Glu Leu Val Leu Ser Leu Met Ile Tyr His Val Asp His Asp Trp Glu
625 630 635 640
Pro Leu Lys Gln Tyr Asn His Pro Asp Val Leu Ser Glu Phe Ser Glu
645 650 655
Thr Val Arg Glu Ile His Asp Ile Ser Gln Leu Ile Val Leu Leu Val
660 665 670
Asp Leu Phe Phe Glu Gln Arg Arg Thr Val Arg Pro Val Glu Glu Ala
675 680 685
Arg Tyr Val Ile Tyr Ser Ile Arg Val Ala Arg Gly Ala Asp Arg Leu
690 695 700
Asp Thr Phe Arg Arg Phe Phe Pro Ala Phe Gly Arg Ala Leu Asp Val
705 710 715 720
Val Asn Arg Ala Thr Asp Val Ala His Ile Thr Ala Leu Asn Phe Leu
725 730 735
Pro Phe Phe Phe Leu Leu Gly Asp Asn Ile Ile Tyr Lys His Arg Glu
740 745 750
Trp Thr Val Pro Val Leu Ile Tyr Thr Glu Gly Val Met Ile Trp Pro
755 760 765
Ala Gln Val Gly Ala Asn Phe Asn Arg Phe Gly Phe Cys Gly Phe Leu
770 775 780
Asn Tyr Met Arg Phe His Ala Gly Ser Arg Leu Arg Ala Lys Pro Leu
785 790 795 800
Asp Asp Ala Glu Lys Ile Val Thr Arg Glu Met Leu Arg Tyr Tyr Met
805 810 815
Lys Thr Asp Ile Phe Asp Gly Gly Val Gln Lys Asn Ile Arg Val Thr
820 825 830
Lys Ser Ser Gln Met Glu Ile Tyr His Ser Ser Leu Cys Gly Gly Leu
835 840 845
Ser Asp Ala Val Val Tyr Val Leu Pro Ile Ser Phe Pro Ile Lys Cys
850 855 860
Leu Thr Leu Ile Ile Val Gly Asp Asp Leu Val Glu Pro Gln Met Arg
865 870 875 880
Phe Gly Arg Val Met Asp Phe Phe Arg His Val Ala Glu His Val Arg
885 890 895
Gly Val Ala Ser Ile Ser Ile Ser Arg Asn Gly Asp Ile Thr Thr His
900 905 910
Ser Gln Gly Ile Val Arg Val Glu Leu Leu Lys Lys Asn Ile Leu Lys
915 920 925
Tyr Gln Phe Gln Val Ala Leu Leu Lys Val Lys Gly Phe Val Phe Gly
930 935 940
Asn Asp Glu Ile Leu Ile Lys Leu Leu Asn Val
945 950 955
<210> 27
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV9 南非 CAE53024的VP5
<400> 27
Met Gly Lys Val Ile Arg Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ser
65 70 75 80
Gly Glu Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Thr Lys Leu Arg Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Asp Glu Ile Lys Arg Lys Phe Gly Glu Glu Leu Glu
115 120 125
Gln Val Tyr Asp Phe Met Asn Gly Val Ala Lys Asp Glu Glu Gly Glu
130 135 140
Glu Lys Gln Phe Asp Ile Leu Asn Lys Ala Val Asn Ser Tyr Asp Lys
145 150 155 160
Ile Leu Ala Glu Glu Asp Thr Gln Met Arg Arg Leu Ala Thr Ala Leu
165 170 175
Gln Lys Glu Ile Gly Asp Arg Thr His Thr Glu Thr Thr Met Val Lys
180 185 190
Glu Tyr Arg Ser Lys Ile Glu Ala Leu Lys Asn Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Thr His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Ser Val Val Ser Thr Ile Leu Glu
275 280 285
Tyr Arg Ala Arg Asp Val Pro Asp Lys Ala Leu Ala Val Ser Val Leu
290 295 300
Ser Lys Asn Arg Thr Ile Gln Glu Asn His Lys Glu Leu Ile His Ile
305 310 315 320
Lys Asp Glu Ile Leu Pro Arg Phe Lys Lys Ala Met Glu Glu Glu Lys
325 330 335
Glu Ile Cys Gly Ile Glu Asp Lys Thr Ile His Pro Lys Val Met Met
340 345 350
Lys Phe Lys Ile Pro Arg Thr Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Ile Ser His
370 375 380
His His Ala Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Ser Ile Asp
385 390 395 400
Leu Val His Tyr Glu Asp Leu Thr Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Thr Ala Ala Gly Arg Thr Leu Thr Glu Ala Tyr Lys Glu Phe
420 425 430
Leu Asn Leu Ala Ile Ser Asn Asn Tyr Gly Thr Gln Met His Thr Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Leu
450 455 460
His Tyr Asp Val Ser Phe Ala Asp Leu Arg Gly Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Cys Lys Ile
500 505 510
Leu Gly Asp Gln Ile Asp Ile Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 28
<211> 955
<212> PRT
<213> 人工序列
<220>
<223> BTV8 意大利 AEO19795的VP2
<400> 28
Met Asp Glu Phe Gly Ile Pro Ile Tyr Leu Ser Ser Ser Val Pro His
1 5 10 15
Asp Leu Leu Ser Asp Tyr Asp Phe Met Ile Asp Leu Ser Asp Lys Val
20 25 30
Val His Ala Gly Asp Arg His Asp Pro Lys Lys Leu Lys Glu Arg Lys
35 40 45
Ala Ile Asp Val Pro Asp Gly Glu Ala Arg Thr Asp Leu Asn Tyr Asn
50 55 60
Pro Thr Asp Asn Glu Gly Asn Gln Leu Pro Arg Ala Leu Asp Ile Ala
65 70 75 80
Leu Ser Ala Arg Leu Thr Arg Lys Ser Val Arg Asn Asn Asp Gly Leu
85 90 95
Asp Phe Leu Thr Asp Asp Lys Trp Met Glu Trp Met Leu Ser Asp Ala
100 105 110
Met Asp Val Gln Pro Leu Lys Ile Asp Leu Ser Ala Arg Asn Gly Phe
115 120 125
Val Lys Cys Asp Ile Phe Asn Ser Ser Ile Tyr Ile Arg Arg Lys Tyr
130 135 140
Ala Asp Ala Ile Ala Tyr Arg Tyr Thr Ser Leu Glu Asp Glu Ser Lys
145 150 155 160
Gly Cys Asn His Thr Arg Val Cys Met Val Asn His Leu Ile Asn Asn
165 170 175
Gly Leu Tyr Asn Ala Val Gln Glu Cys Ala Tyr Ala Leu Lys Asp Thr
180 185 190
Tyr Val Leu Arg Val His Ser Gln Arg Glu Arg Ile Asp Glu Pro Phe
195 200 205
Glu Pro Gly Arg Pro Lys Ile Gly Ser Leu Gly Arg Asn Ala Arg Ile
210 215 220
Asp Met Ser Asp Pro Gly Tyr Ser Leu Phe Lys Gly Gly Met Leu Gln
225 230 235 240
Ile Thr Val Ser Gly Glu Ile Pro Asn Asp Ile Arg Val Glu Met Glu
245 250 255
Arg Leu Asn Gln Ile Arg Ala Thr Trp Ile Arg Asp Lys Ser Ser Arg
260 265 270
Glu Val Arg Ala Ile Glu Leu Cys Thr Leu Leu Ser Arg Ile Gly Arg
275 280 285
Val Lys Trp Asn Ala Glu Glu Glu Pro Lys Asp Glu Gly Ala Met Ser
290 295 300
Leu Arg Phe Gln Asn Lys Ile Asp Ser Met Phe Leu Ser Glu Asn Thr
305 310 315 320
Glu Lys Thr Asn Ile Met Gln Asn Thr Ser Gly Arg Thr Asp Glu Glu
325 330 335
Arg Phe Tyr Ala Leu Leu Leu Ile Cys Ala Thr Asp Ala Tyr Ser Arg
340 345 350
Arg Ile Trp Arg Ser Asn Pro Tyr Pro Cys Leu Arg Gly Thr Leu Ile
355 360 365
Ala Ala Glu Cys Val Leu Gly Asp Pro Tyr Lys Thr Leu Arg Arg Lys
370 375 380
Phe Asn Trp Ser Val Arg His Ala Ala Asp Lys Ala Leu Glu Asn Asn
385 390 395 400
Ala Tyr Ile Phe Thr Arg Ile Asn Leu Phe Asp Thr Glu Arg Thr Pro
405 410 415
Gly Met Arg Val Val His Trp Thr Gln Glu Leu Thr Met Glu Ser Gln
420 425 430
Thr Thr Trp Asp Glu Gly Tyr Pro Leu Lys Asp Glu Ala Pro Asp Asp
435 440 445
Glu Met His Cys Lys Ile Asp Thr Asn Lys Tyr Lys Glu Met Val Thr
450 455 460
Gln Val Ile Asp Gly Gly Trp Asp Gln Glu Asn Phe Lys Ile His Lys
465 470 475 480
Leu Phe His Asp Asp Gly Asn Ile Phe Leu Met Asp Phe Glu Lys Asp
485 490 495
Ala Lys Leu Thr Ala Gln Ser Glu Val Gln Tyr Pro Asp Tyr Tyr Asp
500 505 510
Lys Trp Ile Tyr Ala Pro Ile Phe Asp Thr Lys Tyr Lys Ile Thr Glu
515 520 525
Thr Glu Ile Ala Asn Ala Lys Asn Leu Asp Pro Ala Ile Lys Arg Thr
530 535 540
Ile Asp Pro Met Met Asp Asp Pro Val Met Leu Gln Leu Ser Thr Ile
545 550 555 560
Gly Asn Leu Tyr Asp Thr Arg Pro Ala Val Met Gly Ser Thr Leu Ser
565 570 575
Met Arg Gln Arg Gln Thr Ser Leu His Lys Thr Leu Leu Asp Asp Pro
580 585 590
Gln Asn Glu Arg Phe Tyr Ser Ser Arg Ser Ile Lys Lys Val Asp Asp
595 600 605
Pro Cys Pro Val Tyr Tyr Thr Ala Ser Leu Val Leu Gln Lys Ile Ser
610 615 620
Glu Leu Val Met Ser Leu Met Ile Tyr His Val Asp Gln Asp Trp Glu
625 630 635 640
Ser Leu Lys Gln Tyr Lys His Pro Ser Ile Leu Ser Asp Phe Ser Ser
645 650 655
Leu Val Ser Asn Ile Gln Asp Ile Ser Gln Leu Val Val Leu Leu Val
660 665 670
Asp Phe Phe Phe Glu Arg Arg Ser Val Leu Arg Ser Val Glu Glu Ser
675 680 685
Arg Tyr Ile Ile His Ile Ile Arg Ser Ser Asn Asp Ala Asn Arg Leu
690 695 700
Lys Ser Phe Glu Leu Tyr Phe Pro Arg Phe Gly Gly Ile Leu Arg Thr
705 710 715 720
Ile Lys Asp Ala Lys Glu Val Ser His Val Thr Ala Leu Asn Phe Leu
725 730 735
Pro Tyr Phe Phe Leu Leu Gly Asp Asn Ile Ile Tyr Lys His Lys Glu
740 745 750
Trp Ser Val Pro Val Leu Ile Tyr Ser Asp Gly Leu Met Ile Trp Pro
755 760 765
Ala Gln Val Gly Ala Asn Phe Asn Arg Phe Gly Phe Phe Gly Phe Leu
770 775 780
Asn Tyr Met Arg Phe His Pro Gly Ile Arg Leu Arg Ser Lys Val Leu
785 790 795 800
Asp Asp Ala Glu Lys Val Val Ala Val Glu Met Leu Asn Tyr Tyr Met
805 810 815
Arg Thr Asp Ile Phe Lys Gly Gly Ile Gln Lys Asn Val Arg Val Thr
820 825 830
Lys Leu Ser Gln Met Glu Ile Tyr His Ser Ser Leu Cys Gly Gly Leu
835 840 845
Ser Asp Ala Leu Ile Tyr Ala Leu Pro Ile Ser Phe Pro Ile Lys Cys
850 855 860
Leu Thr Leu Ile Ile Val Gly Asp Asp Leu Ala Glu Pro Gln His Arg
865 870 875 880
Ile Ser Lys Ala Leu Glu Phe Phe Glu His Val Arg Asp His Val Arg
885 890 895
Gly Ile Ala Ser Ile Ser Ile Ser Arg Asn Gly Asp Val Thr Thr His
900 905 910
Ser Gln Gly Ile Val Lys Val Glu Leu Leu Lys Lys Asn Ile Leu Arg
915 920 925
His Gln Phe Arg Val Ala Leu Leu Lys Val Lys Gly Tyr Val Phe Gly
930 935 940
Asn Asp Glu Met Leu Thr Lys Leu Leu Asn Val
945 950 955
<210> 29
<211> 956
<212> PRT
<213> 人工序列
<220>
<223> BTV10 CAE51097的VP2
<400> 29
Met Glu Glu Phe Val Ile Pro Val Phe Ser Glu Arg Asp Ile Pro Tyr
1 5 10 15
Ser Leu Leu Asn His Tyr Pro Leu Ala Ile Arg Ile Asp Val Lys Val
20 25 30
Asp Asp Glu Asp Gly Arg His Asn Leu Ile Lys Ile Pro Glu Ser Asp
35 40 45
Met Ile Asp Val Pro Lys Leu Ser Val Ile Glu Ala Leu Asn Tyr Arg
50 55 60
Pro Lys Arg Asn Asp Gly Val Val Val Pro Arg Leu Leu Asp Ile Thr
65 70 75 80
Leu His Ala Tyr Asp Lys Arg Lys Ser Thr Lys Ser Ala Lys Gly Val
85 90 95
Glu Phe Thr Thr Asp Ala Lys Trp Met Lys Trp Ala Ile Asp Asp Lys
100 105 110
Met Asp Ile Gln Pro Leu Lys Val Thr Leu Asp Asn His Tyr Ser Val
115 120 125
Asn His Gln Leu Phe Asn Cys Ile Val Lys Ala Arg Ser Ala Asn Ala
130 135 140
Asp Thr Ile Tyr Tyr Asp Tyr Tyr Pro Leu Glu Asn Gly Ala Lys Lys
145 150 155 160
Cys Asn His Thr Asn Leu Asp Leu Leu Arg Ser Leu Thr Thr Thr Glu
165 170 175
Met Phe His Ile Leu Gln Gly Ala Ala Tyr Ala Leu Lys Thr Thr His
180 185 190
Glu Leu Val Ala His Ser Glu Arg Glu Ser Thr Ser Glu Thr Tyr Gln
195 200 205
Val Gly Thr Gln Arg Trp Ile Gln Leu Arg Lys Gly Thr Lys Ile Gly
210 215 220
Tyr Arg Gly Gln Pro Tyr Glu Arg Phe Ile Ser Ser Leu Val Gln Val
225 230 235 240
Ile Ile Lys Gly Arg Val Pro Asp Glu Ile Arg Asp Glu Ile Ala Glu
245 250 255
Leu Asn Arg Ile Lys Asp Glu Trp Lys Asn Ala Ala Tyr Asp Arg Thr
260 265 270
Lys Ile Arg Ala Leu Glu Leu Cys Lys Ile Leu Ser Ala Ile Gly Arg
275 280 285
Lys Met Leu Asp Val Gln Glu Glu Pro Lys Asp Glu Met Ala Leu Ser
290 295 300
Thr Arg Phe Gln Phe Lys Leu Asp Glu Lys Phe Ile Arg Thr Asp Gln
305 310 315 320
Glu His Val Asn Ile Phe Glu Val Gly Gly Pro Ala Thr Asp Asp Gly
325 330 335
Arg Phe Tyr Ala Leu Ile Ala Ile Ala Ala Thr Asp Thr Gln Gln Gly
340 345 350
Arg Val Trp Arg Thr Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu Ile
355 360 365
Ala Ala Glu Cys Glu Leu Gly Asp Val Tyr Phe Thr Leu Arg Gln Thr
370 375 380
Tyr Lys Trp Ser Leu Arg Ser Glu Tyr Gly Gln Arg Glu Arg Pro Leu
385 390 395 400
Glu Asp Asn Lys Tyr Val Phe Ser Arg Leu Asn Leu Phe Asp Thr Asn
405 410 415
Leu Ala Ile Gly Asp Glu Ile Ile His Trp Arg Tyr Glu Ile Tyr Arg
420 425 430
Pro Lys Glu Thr Thr His Asp Asp Gly Tyr Ile Cys Val Ser Gln Lys
435 440 445
Asp Asp Asp Glu Leu Leu Cys Glu Ile Asp Gly Asp Arg Tyr Lys Glu
450 455 460
Met Phe Asp Arg Met Ile Gln Gly Gly Trp Asp Gln Glu Arg Phe Lys
465 470 475 480
Leu His Asn Ile Leu Thr Glu Pro Asn Leu Leu Thr Ile Asp Phe Glu
485 490 495
Lys Asp Ala Tyr Leu Gly Ser Arg Ser Glu Leu Val Phe Pro Pro Tyr
500 505 510
Tyr Asp Lys Trp Ile Asn Ser Pro Met Phe Asn Ala Lys Leu Lys Ile
515 520 525
Ala Arg Gly Glu Ile Ala Thr Arg Lys Val Asp Asp Pro Trp Asn Asn
530 535 540
Arg Ala Val His Gly Tyr Ile Lys Thr Ser Ala Glu Ser Leu Gly Tyr
545 550 555 560
Val Leu Gly Pro Tyr Tyr Asp Leu Arg Leu Gln Leu Phe Gly Asp Ala
565 570 575
Leu Ser Leu Glu Gln Lys Gln Ser Ala Val Phe Glu Tyr Met Ala Gln
580 585 590
Gln Asp Asp Phe Pro Ala Leu Thr Asp Tyr Thr Lys Glu Lys Asn Gly
595 600 605
Cys Pro His Ser Gly Gly Thr Phe Tyr Thr Phe Arg Lys Val Ala Leu
610 615 620
Ile Ile Leu Ser Ser Tyr Glu Arg Leu Asp Pro Ser Leu His Glu Gly
625 630 635 640
Arg Glu His Glu Thr Tyr Met His Pro Ala Ile Asn Asp Val Phe Arg
645 650 655
Arg Tyr Ala Leu Glu Met Lys Glu Phe Ser Gln Leu Ile Cys Phe Val
660 665 670
Phe Asp Tyr Ile Phe Glu Lys His Val Gln Leu Arg Asn Ala Lys Glu
675 680 685
Ala Arg Arg Ile Ile Tyr Leu Ile Gln Asn Thr Ser Gly Val His Arg
690 695 700
Leu Asp Ile Leu Arg Glu Thr Phe Pro Asn Phe Leu Arg His Val Met
705 710 715 720
Asn Leu Arg Asp Val Lys Arg Ile Cys Asp Leu Asn Val Ile Asn Phe
725 730 735
Phe Pro Leu Leu Phe Leu Ile Gln Asp Asn Ile Ser Tyr Trp His Arg
740 745 750
Gln Trp Ser Ile Pro Met Ile Leu Phe Gly Glu Val Val Arg Leu Ile
755 760 765
Pro Ile Glu Val Gly Ala Tyr Ala Asn Arg Phe Gly Phe Lys Ser Phe
770 775 780
Leu Asn Phe Ile Arg Phe His Pro Gly Asp Ser Lys Lys Lys Gln Asp
785 790 795 800
Ala Asp Asp Thr His Lys Glu Phe Gly Ser Ile Cys Phe Glu Tyr Tyr
805 810 815
Thr Thr Thr Lys Ile Ser Gln Gly Glu Ile Asp Val Pro Val Ile Thr
820 825 830
Ser Lys Leu Asp Thr Leu Lys Leu His Ile Ala Ser Leu Cys Ala Gly
835 840 845
Leu Ala Asp Ser Leu Val Tyr Thr Leu Pro Val Ala His Pro Lys Lys
850 855 860
Ser Ile Val Leu Ile Ile Val Gly Asp Asp Lys Leu Glu Pro Gln Val
865 870 875 880
Arg Ser Glu Gln Ile Val Asn Lys Tyr His Tyr Ser Arg Arg His Ile
885 890 895
Ser Gly Val Val Ser Ile Cys Val Asp Gln Asn Gly Gln Leu Lys Val
900 905 910
His Ser Met Gly Ile Thr Arg His Arg Ile Cys Asp Lys Ser Ile Leu
915 920 925
Lys Tyr Lys Cys Lys Val Val Leu Val Arg Met Pro Gly His Val Phe
930 935 940
Gly Asn Asp Glu Leu Met Thr Lys Leu Leu Asn Val
945 950 955
<210> 30
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV10 CAE53025的VP5
<400> 30
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Val Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ala Ala Thr Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Thr
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Met Gln
85 90 95
Met Lys Ile Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Lys Glu Ile Thr Glu Lys Phe Gly Lys Glu Leu Gly
115 120 125
Glu Val Tyr Asp Phe Met Asn Gly Glu Ala Lys Glu Val Glu Ala Val
130 135 140
Glu Glu Gln Tyr Thr Met Leu Cys Lys Ala Val Asp Ser Tyr Glu Lys
145 150 155 160
Ile Leu Lys Glu Glu Asp Ser Lys Met Ala Ile Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Ala Glu Arg Ser Glu Asp Glu Ile Lys Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Ser Ala Ile Glu Ile Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Met Arg Ser Pro Lys Ile Glu Pro Thr Ile Ile Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Lys Asp Ile Pro Asp Glu Gln Leu Ala Val Ser Val Leu
290 295 300
Asn Lys Lys Thr Ala Val Ala Asp Asn Cys Ser Glu Ile Ala His Ile
305 310 315 320
Lys Gln Glu Ile Leu Pro Lys Phe Lys Gln Ile Met Asn Glu Glu Lys
325 330 335
Glu Ile Glu Gly Ile Glu Asp Lys Val Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Ile Pro Arg Thr Gln Gln Pro Gln Ile His Ile Tyr Ala
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Arg Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Thr Ser His Trp His Ala Leu Gly Met
405 410 415
Ala Gln Glu Ala Ser Gly Arg Thr Leu Thr Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ser Ile Ser Ser Thr Phe Ser Ser Ala Ile His Ala Arg
435 440 445
Arg Met Ile Arg Ser Arg Ala Val His Pro Ile Phe Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Ala Leu Lys Asn Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Ile
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 31
<211> 956
<212> PRT
<213> 人工序列
<220>
<223> BTV11 CAE51098的VP2
<400> 31
Met Glu Glu Phe Val Ile Pro Val Tyr Ser Glu Thr Asp Ile Pro Tyr
1 5 10 15
Ser Leu Leu Ser His Tyr Pro Leu Ala Ile Arg Thr Asp Val Lys Ile
20 25 30
Ala Asp Thr Asp Glu Gly His Asp Val Val Lys Ile Pro Glu Ser Asp
35 40 45
Met Ile Asp Val Pro Arg Val Asp Ile Val Glu Ala Leu Ala Ala Lys
50 55 60
Pro Met Arg Asn Asp Gly Ile Val Val Pro Arg Leu Leu Asp Ile Thr
65 70 75 80
Leu Arg Ala Tyr Asp Asp Arg Lys Ala Ile Lys Ser Ala Arg Gly Val
85 90 95
Glu Phe Met Thr Asn Ala Lys Trp Met Lys Trp Ala Ile Asp Asp Arg
100 105 110
Met Asp Ile Gln Pro Leu Lys Val Ala Ile Asp His Tyr Asn Ala Val
115 120 125
Asn His Gln Leu Phe Asn Cys Ile Val Lys Ala Arg Ser Ala Asn Ala
130 135 140
Asp Thr Ile Tyr Tyr Asp Tyr Phe Pro Leu Glu Ser Lys Val Lys Lys
145 150 155 160
Cys Asn His Ala Asn Leu Asp Leu Leu Arg Ser Leu Thr Asn Thr Glu
165 170 175
Met Phe His Met Leu Gln Gly Ala Ala Tyr Ser Leu Lys Ser Asn Tyr
180 185 190
Glu Leu Ile Thr Asn Ser Glu Arg Asn Ser Thr Glu Glu Thr Tyr Ala
195 200 205
Pro Gly Val His Asn Gln Ile Arg Leu Val Arg Gly Thr Arg Ile Gly
210 215 220
Tyr Lys Gly Glu Ala Tyr Ser Arg Phe Val Ser Ser Leu Val Gln Val
225 230 235 240
Arg Ile Gln Gly Arg Thr Pro Pro Glu Ile Val Asp Asp Ile Ala Arg
245 250 255
Leu Asn Val Ile Arg Thr Glu Trp Ile Asn Ala Gln Phe Asp Ser Thr
260 265 270
Lys Ile Arg Ala Leu Glu Leu Cys Lys Ile Leu Ser Ala Ile Gly Arg
275 280 285
Lys Met Leu Asn Thr His Glu Glu Pro Lys Asp Glu Met Asp Leu Ser
290 295 300
Thr Lys Phe Gln Phe Lys Leu Asp Asp Lys Phe Lys Lys Thr Asp Ser
305 310 315 320
Glu His Ile Asn Ile Phe Asn Val Arg Ala Pro Ala Thr His Glu Gly
325 330 335
Arg Phe Tyr Ala Leu Ile Ala Ile Ala Ala Thr Asp Thr Gln Arg Gly
340 345 350
Arg Ile Trp Arg Thr Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu Ile
355 360 365
Ala Ala Glu Cys Glu Leu Gly Asp Val Tyr His Thr Leu Arg Gln Val
370 375 380
Tyr Lys Trp Ser Leu Arg Gln Asp Tyr Gly Arg Thr Glu Val Pro Leu
385 390 395 400
Glu Asn Asn Lys Tyr Val Phe Ser Arg Ile Asn Leu Phe Asp Ser Asn
405 410 415
Leu Asp Val Gly Asp Gln Val Val His Trp Met Tyr Glu Val Asp Gly
420 425 430
Pro Ala Glu Thr Thr Tyr Asp Asn Gly Tyr Met Cys Lys Thr Glu Arg
435 440 445
Glu Asp Glu Glu Leu Val Cys Lys Ile Ser Glu Glu Lys Tyr Lys Thr
450 455 460
Met Leu Asp Arg Met Ile Gln Gly Gly Trp Asp Gln Glu Arg Phe Lys
465 470 475 480
Leu Tyr Ser Ile Leu Thr Asp Pro Asn Leu Leu Thr Ile Asp Phe Glu
485 490 495
Lys Asp Ala His Leu Asn Ile Arg Ser Glu Phe Val Leu Pro Ser Tyr
500 505 510
Phe Asp Gln Trp Ile Tyr Ser Pro Met Phe Asn Ala Arg Leu Arg Ile
515 520 525
Thr His Gly Glu Ile Ala Thr Arg Lys Ser Ala Asp Pro Trp Asn Lys
530 535 540
Arg Val Val Phe Gly Tyr Ile Lys Ala Ser Thr Glu Ser Pro Glu Tyr
545 550 555 560
Ala Leu Gly Gln Tyr Phe Asp Met Arg Ile Gln Phe Tyr Gly Asp Ala
565 570 575
Leu Ser Ser Lys Gln Asn Gln Ser Ala Val Phe Gln Tyr Leu Ser Gln
580 585 590
Gln Glu Asp Phe Pro Thr Leu Thr Ser Tyr Ala Lys Gly Asp Val Val
595 600 605
Cys Pro His Ser Gly Gly Ala Leu Tyr Thr Phe Arg Arg Val Ala Leu
610 615 620
Met Leu Met Ala Asn Tyr Glu Lys Leu Ser Pro Asp Leu His Glu Gly
625 630 635 640
Met Glu Asp Tyr Thr Tyr Thr His Pro Ser Ile Gly Gly Ala Tyr Gln
645 650 655
Lys Arg Ile Leu Glu Met Arg Asp Phe Ser Gln Leu Ile Cys Phe Ile
660 665 670
Ile Asp Tyr Ile Phe Glu Arg His Asp Gln Leu Arg Asp Ala Lys Glu
675 680 685
Ala Arg Arg Ile Leu Tyr Leu Ile Gln Asn Leu Asp Glu Pro Gln Arg
690 695 700
Leu Glu Met Leu Asn Val Thr Phe Pro Asn Phe Phe Arg His Phe Leu
705 710 715 720
Lys Leu Lys Asp Val Gln Leu Ile Ser Asp Leu Asn Val Ile Asn Phe
725 730 735
Leu Pro Leu Leu Phe Leu Val Gln Asp Asn Ile Ser Tyr Trp His Arg
740 745 750
Gln Trp Ala Val Pro Met Ile Leu Tyr Asp Asp Val Ile Arg Leu Ile
755 760 765
Pro Val Glu Val Gly Ala Tyr Ala Asn Arg Phe Gly Ile Lys Ser Phe
770 775 780
Phe Asn Phe Thr Arg Phe His Pro Gly Asp Ala Lys Lys Arg Gln Lys
785 790 795 800
Ala Asp Asp Thr His Lys Glu Phe Gly Ser Ile Ser Phe Asn Tyr Tyr
805 810 815
Ala Ser Thr Lys Ile Ala Gln Gly Gly Val His Thr Pro Val Val Thr
820 825 830
Thr Lys Leu Asp Thr Leu Lys Ile His Leu Ser Ser Leu Cys Ala Gly
835 840 845
Leu Ala Asp Ser Ile Val Tyr Thr Leu Pro Val Ala His Pro Lys Lys
850 855 860
Cys Ile Val Leu Ile Ile Val Gly Asp Asp Lys Leu Glu Pro His Val
865 870 875 880
Arg Ser Glu Gln Val Val Ser Lys Tyr Tyr Phe Ser Arg Arg His Val
885 890 895
Ser Gly Ile Val Ser Ile Cys Val Gly Gln Asp Asn Gln Leu Lys Val
900 905 910
Tyr Ser Ser Gly Ile Val Arg His Arg Val Cys Glu Lys Phe Ile Leu
915 920 925
Lys Tyr Lys Cys Lys Val Val Leu Val Lys Met Pro Gly Tyr Val Phe
930 935 940
Gly Asn Asp Glu Leu Met Thr Lys Leu Leu Asn Val
945 950 955
<210> 32
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV11 CAE53026的VP5
<400> 32
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Ser Ala Leu Ser Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ala Ala Thr Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Thr
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Met Gln
85 90 95
Thr Lys Ile Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Lys Glu Ile Thr Lys Glu Phe Gly Lys Glu Leu Glu
115 120 125
Glu Val Tyr Asp Phe Met Asn Gly Glu Ala Lys Glu Glu Glu Val Val
130 135 140
Gln Glu Gln Tyr Ser Met Leu Cys Lys Ala Val Asp Ser Tyr Glu Lys
145 150 155 160
Ile Leu Lys Ala Glu Asp Ser Lys Met Ala Ile Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Ser Glu Arg Ser Gln Asp Glu Ile Lys Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Asn Ala Ile Glu Ile Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Met Arg Ser Pro Lys Ile Glu Pro Thr Ile Ile Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Lys Glu Ile Pro Asp Glu Gln Leu Ala Val Ser Val Leu
290 295 300
Asn Lys Lys Thr Ala Val Thr Asp Asn Cys Asn Glu Ile Ala His Ile
305 310 315 320
Lys Gln Glu Ile Leu Pro Lys Phe Lys Gln Ile Met Asp Glu Glu Lys
325 330 335
Glu Ile Glu Gly Ile Glu Asp Lys Val Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Ile Pro Arg Thr Gln Gln Pro Gln Ile His Ile Tyr Ala
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Arg Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Thr Ser His Trp His Ala Leu Gly Leu
405 410 415
Ala Gln Glu Ala Ser Gly Arg Thr Leu Thr Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ser Ile Ser Ser Thr Tyr Ser Ser Ala Ile His Ala Arg
435 440 445
Arg Met Ile Arg Ser Arg Ala Val His Pro Ile Phe Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Ala Leu Lys Asn Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Glu Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Ile
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 33
<211> 950
<212> PRT
<213> 人工序列
<220>
<223> BTV12 CAE51099的VP2
<400> 33
Met Glu Asp Phe Pro Ile Cys Val Ile Glu Arg Glu Ser Lys Phe Asp
1 5 10 15
Glu Asp Phe Val Val Arg Tyr Pro Val Ile Ile Asp Leu Lys Glu Cys
20 25 30
Val Asp Asp Val Asn Gly Lys Thr Asp Val Ser Lys Leu Glu Glu Thr
35 40 45
Arg Gly Ile Phe Val Arg Ala Glu Gly Ile Arg Val Ala Leu Asn Ser
50 55 60
Arg Pro Asp Asn Leu Ser His Val Ile Thr Pro Ser Ala Leu Asp Val
65 70 75 80
Leu Ile Arg Ala Tyr Asp Arg Lys His Lys Ile Ala Leu Asp Lys Arg
85 90 95
Thr Asp Asp Ser Gly Arg Met Ala Asp His Asn Ile Asp Val Trp Val
100 105 110
Lys Lys Arg Leu Glu Glu Gln Ala Asp Leu Gln Ala Ile Tyr His Asp
115 120 125
Val Asp Leu Gly Arg Gly Lys Leu Lys Phe Lys Met Leu Thr Gly Leu
130 135 140
Val Lys Leu Asp Ser Asn Tyr Ala Glu Thr Val Gly Tyr Asp Tyr Ala
145 150 155 160
Pro Val Pro Lys Val Gly Cys Asp His Gly Asn Val Asp Phe Leu Tyr
165 170 175
Asp Phe Ile Leu Ser Gly Ser Leu Gln Val Ser Lys Thr Ala Gly Tyr
180 185 190
Thr Leu Lys Gln Thr Tyr Arg Leu Thr Val His Ala Glu Ala Asn Ala
195 200 205
Glu Thr Arg Asp Gln Leu Ile Val Asn Gly Val Tyr Thr Glu Gly Val
210 215 220
Thr Arg Gly Glu Glu Ile Arg Asp Met Lys Lys Met Cys Tyr Ser Arg
225 230 235 240
Phe Arg Asp Gly Trp Ile Arg Ala Thr Ile Gln Pro Lys Ile Val Glu
245 250 255
Glu Leu Arg Arg Ser Lys Glu Ala Leu Asp Arg Ile Ser Ser Glu Trp
260 265 270
Tyr Glu Lys Leu Thr Thr Pro Asp Val Val Glu Ile Cys Lys Ile Val
275 280 285
Ser Ser Ile Gly Arg Gln Met Trp Asn Ser Glu Glu Leu Pro Val Asp
290 295 300
Asp Ala Met Arg Ser Lys Val Phe Gln Glu Glu Leu Arg Leu Leu Phe
305 310 315 320
Arg Val Gly Asn Ser Glu Tyr Asp Thr Ile Gln Ala Ile Gln Ser Gly
325 330 335
Gln Thr Pro Leu Lys Lys Phe Tyr Ala Leu Leu Ala Ile Ala Ala Thr
340 345 350
Asp Ser Tyr Arg Trp Arg Ile Trp Trp Ser Asn Pro Tyr Pro Cys Leu
355 360 365
Arg Gly Thr Ile Ile Ala Cys Glu Met Glu Leu Gly Asp Val Tyr Glu
370 375 380
Thr Leu Arg Ser Thr Phe His Trp Ser Leu Arg Pro Thr Tyr Ala Thr
385 390 395 400
Arg Lys Glu Ile Asp Arg Glu Arg Arg Ala Tyr Pro Tyr Gln Arg Ile
405 410 415
Asn Leu Phe Glu Ser Arg Leu Asp Pro Gly Thr Arg Ile Ile Asn Trp
420 425 430
Leu Val Val Arg Glu Pro Leu Ala Glu Pro Asp Val Lys Ser Gly Arg
435 440 445
Val Cys Met Thr Ser Thr Glu Ser Glu Tyr Ile Leu Lys Val Asp Asp
450 455 460
Thr Lys Tyr Arg Glu Met Ile Gly Gln Ile Ile Glu Arg Gly Trp Glu
465 470 475 480
Lys Glu His Leu Lys Leu Glu Lys Ile Val Gln Asp Glu Gly Asn Val
485 490 495
Phe Gln Met Glu Phe Glu Lys Asp Ala Ser Leu Asp Glu Arg Ser His
500 505 510
Leu Val Met Pro Tyr Tyr Tyr Asp Lys Glu Ile His Cys Pro Met Tyr
515 520 525
His Lys Lys Ile Lys Ile Thr Glu Ala Glu Ile Ala Thr Ser Trp Ser
530 535 540
Asp Asp Pro Trp Leu Asn Arg Val Leu Asn Gly Phe Leu Gly Asp His
545 550 555 560
Val Glu Thr Phe Asn Leu Gly Cys Asn His Ile Phe Asp Met Arg Val
565 570 575
Pro Leu Lys Gly Asn Leu Leu Thr Gln Glu Gln Ala Ile Ser Ser Tyr
580 585 590
His Glu His Ile Arg Ser Pro Glu Ala Gln Ser Glu Asn Gln Cys Ala
595 600 605
Leu Lys Val Tyr Ala Asp Glu Ala Val Ser Lys Leu Phe Thr Thr Leu
610 615 620
Tyr His Arg Leu Ala Tyr His Leu Pro Lys Thr Glu Tyr Ile Glu Arg
625 630 635 640
Glu Glu Glu Val Ser Gln Tyr Glu Gly Leu Val Lys Arg Ile Arg Thr
645 650 655
Trp Asp Gln Phe Ile Ile Phe Val Phe Asp Phe Phe Phe Glu Asn Arg
660 665 670
Lys Gly Leu Arg Thr Thr Glu Glu Ala Thr Gln Leu Ala Lys Ala Ile
675 680 685
Arg Ser Asn Val Gly Ala Thr Arg Trp Thr Met Met Ala Ser Ala Phe
690 695 700
Pro Thr Ser Val Arg Leu Val Lys Gly Ile Leu Arg Ala Lys Thr Trp
705 710 715 720
Gly Glu Cys Tyr Ile Met Asn Phe Leu Pro Leu Leu Ile Cys Gly Gly
725 730 735
Ser Ser Ile Ser Tyr Leu His Arg Glu Trp Ser Tyr His Met Ile Val
740 745 750
Val Phe Glu Asp Gly Ile Lys Val Val Pro Ala Met Thr Gly Arg Ser
755 760 765
Leu Thr Gln Gly Ser Leu Gly Glu Trp Ala Ser Phe Val Cys Phe Phe
770 775 780
Met Gly Ser Asn His Glu Lys Ile Lys Leu Glu Glu Asp Glu Leu Glu
785 790 795 800
Val Leu Arg Lys Cys Val Glu Phe Tyr Glu Gly Val Lys Met Val Asp
805 810 815
Ile Ile Lys Asp Thr Pro Val Arg Met Thr Lys Tyr Ile Ala Cys Asp
820 825 830
Leu Ala Phe Gly Ser Gly Cys Gly Gly Thr Ser Glu Ser Leu Ser Phe
835 840 845
Ile Leu Pro Val Thr His Pro Asn Arg Ser Leu Ile Ile Phe Val Val
850 855 860
Ala Asp Asp Lys Ile Ser Pro Ala Gln His Ala Ala Arg Ala Lys Arg
865 870 875 880
Arg Phe Arg Gly Thr Arg Glu Tyr Val Asn Asp Val Ile Val Leu Gln
885 890 895
Met Lys Arg Pro Gly Thr Val Gly Asn Val Trp Ser Glu Lys His Ser
900 905 910
Lys Val Arg Ile Cys Arg Arg Asn Phe Leu Lys Tyr Asp His Lys Val
915 920 925
Val Leu Thr Lys Phe Ala Gly Leu Val Tyr Gly Asn Tyr Glu Leu Ile
930 935 940
Thr Lys Leu Ala Asn Leu
945 950
<210> 34
<211> 529
<212> PRT
<213> 人工序列
<220>
<223> BTV12 CAE53027的VP5
<400> 34
Met Met Gly Lys Ile Ile Lys Ser Leu Gly Lys Phe Gly Lys Lys Val
1 5 10 15
Gly Asn Ala Leu Thr Ser Lys Thr Ala Gln Lys Ile Tyr Arg Thr Ile
20 25 30
Gly Ser Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala
35 40 45
Ile Asp Gly Leu Ile Gln Gly Ser Val His Ser Ile Met Thr Gly Asp
50 55 60
Ser Tyr Gly Glu Ser Val Lys Gln Ala Val Ile Leu Asn Val Leu Gly
65 70 75 80
Gly Gly Asp Thr Ile Ala Asp Pro Leu Ser Pro Gly Glu Asn Glu Ile
85 90 95
Tyr Arg Lys Leu Lys Gln Leu Glu Asp Glu Gln Lys Gly Glu Ile Val
100 105 110
Arg Thr Lys His Asn Thr Glu Ile Leu Lys Lys Phe Gly Asn Asp Leu
115 120 125
Asp Glu Val Tyr Lys Phe Ala Thr Gln Glu His Lys Val Gly Glu His
130 135 140
Glu Glu Asp Gln Ile Lys Met Leu Glu Lys Ala Leu Lys Ser Tyr Ile
145 150 155 160
Gly Val Phe Lys Ala Glu Asn Asp Ser Ile Asp Lys Leu Lys Lys Ala
165 170 175
Leu Tyr Val Glu Ser Thr Glu Arg Thr Glu Glu Glu Thr Lys Met Ile
180 185 190
Ser Glu Tyr Arg Asp Lys Ile Asp Ala Leu Arg Ser Ala Ile Glu Val
195 200 205
Glu Lys Glu Gly Leu Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met
210 215 220
Ser Ala Glu Val Leu Glu Ala Ala Ala Glu Glu Val Pro Leu Phe Gly
225 230 235 240
Ala Gly Val Ala Thr Ser Ile Ala Thr Ala Arg Ala Ile Glu Gly Ala
245 250 255
Tyr Lys Ile Lys Ala Val Ile Ser Ala Leu Thr Gly Ile Asp Leu Thr
260 265 270
His Leu Asn Val Pro Lys Val Gln Pro Lys Thr Leu Glu Ala Ile Leu
275 280 285
Asp Val Pro Lys Gly Val Asp Ile Pro Asp Leu Arg Leu Thr Glu Gly
290 295 300
Leu Ile Ala Lys Met Asp Lys Ile Ser Glu Asn Ile Ser Glu Val Asp
305 310 315 320
His Ile Gln Asn Val Ile Val Pro Lys Leu Lys Lys Ala Val Glu Glu
325 330 335
Asp Lys Glu Asn Ile Thr Asp Trp Ser Glu Lys Arg Ile His Pro Lys
340 345 350
Thr Ala Gln Arg Phe Lys Ile Pro Gln Thr Gln Lys Pro Ser Met His
355 360 365
Ile Tyr Ala Ala Pro Trp Asp Ser Asp Glu Val Phe Ile Phe His Val
370 375 380
Ile Ser Pro His His Lys Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu
385 390 395 400
Glu Ile Glu Tyr Val Phe Tyr Glu Asp Leu Thr Gln His Trp His Ala
405 410 415
Leu Gly Thr Ala Gln Ser Thr Tyr Gly Arg Thr Phe Arg Glu Ala Tyr
420 425 430
Lys Glu Phe Phe Leu Leu Ala Ser Asn Val Ala Gly Gln Thr Ala Ile
435 440 445
His Gln Arg Arg Leu Leu Arg Ser Arg Ser Asn His Pro Ile Tyr Leu
450 455 460
Gly Met His Asp Tyr Glu Val Ser Tyr Ala Gln Met Lys Met Asn Ala
465 470 475 480
Met Ala Leu Val Ser Asn Glu Asp Leu Gln Met His Ile Leu Arg Gly
485 490 495
Pro Ile His Phe Gln Arg Arg Thr Ile Val Ala Ala Leu Lys Tyr Gly
500 505 510
Ile Lys Ile Leu Thr Gln Pro Asp Asp Val Ser Ile Met Leu Arg Asn
515 520 525
Ala
<210> 35
<211> 959
<212> PRT
<213> 人工序列
<220>
<223> BTV13 CAE51100的VP2
<400> 35
Met Glu Glu Leu Val Ile Pro Val Ile Thr Glu Arg Phe Asp Lys Arg
1 5 10 15
Leu Val Gly Arg Tyr Asp Tyr Val Ile Glu Leu Ala Arg Pro Glu Gly
20 25 30
Asp Glu Trp Ser Gly His Asp Val Thr His Ile Pro Asp Lys Arg Met
35 40 45
Phe Asp Ile Lys Val Gln Pro Ile Arg Asp Ala Ile Asp Tyr Lys Pro
50 55 60
Val Glu Asn Asp Gly Glu Val Leu Pro Arg Ile Leu Asp Met Ser Ile
65 70 75 80
Ala Cys Tyr Asp Met Arg Lys Arg Met Met Lys Arg Asp Gly Val Asp
85 90 95
Phe Val Ser Asn Thr Lys Trp Leu Glu Trp Met Ile Gln Asp Ser Met
100 105 110
Asp Val Gln Pro Leu Arg Val Asp Met Lys Glu Asp His Ser Thr Val
115 120 125
Gln Tyr Asp Met Phe Ser Ala Lys Leu His Val Asp Ser Arg Lys Ala
130 135 140
Asp Thr Thr Ser Tyr Asn Ile Leu Ala Leu Glu Val Lys Lys Gly Ala
145 150 155 160
Gln Cys His His Val His Ala Asn Ile Trp Asn His Met Ile Arg Asn
165 170 175
His Leu Phe His Ala Val Gln Glu Ser Cys Tyr Ile Phe Lys Pro Thr
180 185 190
Tyr Lys Leu Ile Val Asn Ser Glu Arg Arg Thr Pro Asp Glu Asp Phe
195 200 205
Gln Ile Gly Asn Pro Gln Phe Pro Thr Leu Arg Arg Asn Gln Gln Ile
210 215 220
Phe Leu Gly Asp Asp Ala Tyr Lys Lys Thr Ala Arg Gly Leu Val Gln
225 230 235 240
Val Leu Val Asn Gly Val Val Pro Asp Ile Ile Arg Asn Glu Ile Ala
245 250 255
Ala Leu Asp Ala Ile Arg Asp Arg Trp Ile Gln Gly Asn Tyr Glu Arg
260 265 270
Thr His Ile Lys Ser Leu Glu Leu Cys Asn Leu Leu Ser Ala Ile Gly
275 280 285
Arg Lys Met Val Asn Leu Glu Glu Glu Pro Lys Asp Glu Arg Asn Leu
290 295 300
Ser Leu Arg Phe Gln His Lys Leu Asp Asp Lys Phe Ala Lys Asn Asp
305 310 315 320
Gln Glu Arg Asn Val Ile Phe Ala Gln Lys Ser Gln Arg Asn Asp Gln
325 330 335
Asp Arg Phe Tyr Val Leu Met Val Ile Ala Ala Ser Asp Thr Asn Asn
340 345 350
Ser Arg Val Trp Trp Ser Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu
355 360 365
Ile Ala Ala Glu Cys Lys Leu Gly Asp Val Tyr Tyr Lys Leu Arg Ser
370 375 380
Trp Tyr Glu Trp Ser Val Arg Glu Gly Tyr Lys Pro Arg Asp Leu Asp
385 390 395 400
Arg Gln Tyr Glu Lys Tyr Ile Val Gly Arg Val Asn Leu Phe Asp Leu
405 410 415
Glu Ala Glu Pro Gly Thr Lys Val Leu His Trp Glu Tyr Glu Leu Ile
420 425 430
Ser Lys Leu Tyr Ile Val Ser Asn His Glu Gly Asn Gln Cys Asp Leu
435 440 445
Tyr Pro Asp Glu Gly Glu Ile Val Ala Lys Phe Asp Asp Ala Arg Tyr
450 455 460
Ser Asp Met Ile Gln Thr Ile Ile Asn Glu Gly Trp Lys Gln Gly Asp
465 470 475 480
Phe Lys Met Phe Lys Val Leu Lys Asp Glu Gly Asn Pro Leu Leu Tyr
485 490 495
Asp Leu Glu Lys Asp Ile Lys Leu Asp Arg Val Ser Arg Val Val Phe
500 505 510
Pro Pro Tyr Phe Asp Gln Trp Thr Tyr Val Pro Met Phe Asn Ala Arg
515 520 525
Ile Lys Pro Cys Glu Val Glu Leu Ala Glu Arg Lys Asn Thr Asp Pro
530 535 540
Tyr Val Lys Arg Thr Leu Lys Pro Leu Lys Ala Asp Cys Ile Glu Leu
545 550 555 560
Met Arg Tyr His Met Ser Gln Tyr Met Asp Leu Arg Val Ser Leu Gln
565 570 575
Gly Thr Ser Leu Ser Ile Lys Gln Val Pro Ser Ser Ile His Gln Ser
580 585 590
Leu Ala Gln Asp Ala Ser Tyr Ala Gly Ile Leu Ser Arg Arg Arg Glu
595 600 605
Asn Leu Asp Tyr Lys Ser Gln Cys Pro Ile Val Thr Asn Leu Phe Leu
610 615 620
Leu Glu Lys Phe Phe Leu Leu Ile Phe Thr Thr Met Glu Lys His Tyr
625 630 635 640
Trp Glu Met Asp Asp Asp Glu Thr Gln Tyr Glu His Pro Lys Ile Asp
645 650 655
Pro Ser Lys Phe Glu Val Glu Gly Thr Leu His Asp Val Ser Gln Val
660 665 670
Met Val His Leu Leu Asp Arg Phe Phe Glu Lys Arg Arg Phe Leu Arg
675 680 685
Thr Val Asp Glu Ser Arg Trp Ile Leu His Leu Ile Arg Ser Thr Ser
690 695 700
Gly Ser Lys Arg Leu Glu Val Leu Ser Arg Phe Phe Pro Ala Phe Ser
705 710 715 720
Asp Gly Leu Arg Ile Arg Glu Phe Lys Lys Val Arg Asp Ile Met Leu
725 730 735
Leu Asn Phe Leu Pro Phe Leu Phe Leu Thr Gly Asp Asn Met Ala Tyr
740 745 750
Glu His Arg Gln Trp Ala Val Pro Val Ile Phe Tyr Ala Asp Lys Ile
755 760 765
Arg Ile Val Pro Ala Glu Val Gly Ala Tyr Tyr Asn Arg Phe Gly Leu
770 775 780
Thr Cys Ile Leu Glu Leu Met Met Phe Phe Pro Ser Tyr Asp Thr Arg
785 790 795 800
Asn Glu Asp Leu Gly Glu Asp Val Arg Ala Cys Ile Gly Pro Ile Ile
805 810 815
Asn Tyr Tyr Leu Asp Thr Thr Ile Ser Asn Gly Gly Ile Gln Thr Ser
820 825 830
Ile Val Ser Thr Lys Ala Leu Leu Tyr Glu Thr Tyr Leu Ser Ser Ile
835 840 845
Cys Gly Gly Phe Ser Glu Ala Ile Leu Trp Tyr Leu Pro Val Thr His
850 855 860
Pro Ser Lys Cys Leu Ile Ala Leu Glu Val Ser Asp Ala Leu Thr Ser
865 870 875 880
Pro Glu Leu Arg Ile Asp Arg Ile Arg Arg Arg Phe Pro Leu Ser Ser
885 890 895
Asn His Leu Lys Gly Ile Val Gln Val Ser Val Arg Pro Asp Arg Thr
900 905 910
Phe Ser Val Ile Thr Gln Gly Ile Val Lys His Arg Val Cys Lys Lys
915 920 925
Thr Leu Leu Arg His Arg Cys Asp Val Ile Leu Ile Gln Thr Pro Gly
930 935 940
Tyr Val Phe Gly Asn Asp Glu Leu Leu Thr Lys Leu Leu Asn Ile
945 950 955
<210> 36
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV13 AE53029的VP5
<400> 36
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Asn Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ala
65 70 75 80
Gly Asp Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Met Lys Ile Lys Glu Ile Glu Glu Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Ile Lys Tyr Gly Lys Glu Ile Arg Glu Lys Phe Gly Glu Gln Leu Glu
115 120 125
Glu Ile Tyr Gln Phe Met Asn Gly Glu Val Lys Gly Glu Glu Glu Gln
130 135 140
Glu Glu Gln Tyr Lys Val Leu Cys Lys Ala Val Asp Ser Tyr Glu Asn
145 150 155 160
Leu Leu Val Thr Glu Asn Glu Gln Met Arg Thr Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Thr Glu Arg Thr Glu Asn Glu Ser Lys Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Ala Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Val Gly Ser
225 230 235 240
Gly Met Ala Thr Ala Ile Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Thr Met Val Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Lys Glu Ile Pro Asp Lys Glu Leu Ala Val Ser Val Leu
290 295 300
Ala Lys Asn Asp Ala Ile Val Ala Asn Thr Lys Glu Ile Lys His Ile
305 310 315 320
Lys Glu Glu Ile Leu Pro Lys Phe Lys Lys Ile Met Glu Glu Glu Lys
325 330 335
Glu Leu Glu Gly Ile Asp Asp Lys Lys Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Val Pro Arg Ser Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
Phe His Ala Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Ala Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Glu Ala Lys Gly Arg Thr Leu Asn Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ser Ile Gly Ser Thr Phe Thr Ser Pro Met His Ala Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Thr Leu Lys Thr Asn Ala Gln Lys Leu
465 470 475 480
Val Tyr Asp Glu Asp Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Val
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Lys Asn Ala
515 520 525
<210> 37
<211> 955
<212> PRT
<213> 人工序列
<220>
<223> BTV14的VP2
<400> 37
Met Asp Glu Leu Ala Ile Pro Ile Ile Asn Gly His Phe Pro Lys Glu
1 5 10 15
Thr Leu Ile Glu Tyr Asp Cys Ile Ile Glu Leu Asn Pro Pro Val Glu
20 25 30
Asp Asp Ala Ala Asp Val Val Gln Ile Pro Thr Arg Asp Met Met Asp
35 40 45
Ile Pro Asn Val Pro Ile Arg Asp Ala Leu Lys His Leu Pro Gln Arg
50 55 60
Asn Asp Gly His Val Leu Thr Arg Val Leu Asp Ile Ser Leu Ser Gly
65 70 75 80
Tyr Asp Arg Arg Lys Tyr His Lys Thr Asn Ser Gly Gly Glu Phe Tyr
85 90 95
Ser Pro Asn Glu Trp Leu Asn Trp Met Ile Asn Asp Ser Met Asp Val
100 105 110
Gln Pro Leu Lys Val Ser Leu Gly Leu Pro Pro Thr Ile Arg His Glu
115 120 125
Leu Phe Ser Ser Ser Val Phe Ile Ser Ala Lys Lys Ala Asp Thr Thr
130 135 140
Ser Tyr His Ile Glu Pro Ile Cys Glu Lys Asp Lys Ala Cys Asp His
145 150 155 160
Thr Arg Val Ser Met Trp Asn Asn Leu Thr Arg His Glu Met Met His
165 170 175
Cys Ala Gln Glu Ser Ala Tyr Val Leu Lys Pro Thr Tyr Asp Ile Thr
180 185 190
Val Tyr Ser Glu Arg Ala Thr Thr Asp Glu Ala Phe Gln Gln Val Gly
195 200 205
Gln Lys Phe Val Thr Ile Thr Lys Asn His Arg Val Val Leu Gly Asp
210 215 220
Asp Ala Tyr Lys Lys Thr Leu Gly Gly Leu Thr Arg Leu Arg Val Gln
225 230 235 240
Gly Lys Ile Pro Ser Leu Ile Gln Asn Asp Ile Gln Gln Leu Asn Gln
245 250 255
Ile Arg Asp Thr Trp Ile Arg Asp Ser Tyr Asn Pro Arg Asn Ile Arg
260 265 270
Ser Leu Glu Leu Cys Lys Leu Leu Ser Gly Ile Gly Arg Lys Met Val
275 280 285
Cys Ile Glu Glu Glu Pro Lys Asn Glu Ser Asp Leu Ser Val Lys Phe
290 295 300
Gln Phe Lys Leu Asp Glu Lys Phe Arg Leu Asp Asp Ser Glu Arg Gly
305 310 315 320
Val Ile Phe Thr Asn Lys Gly Gln Arg Asn Asp Gln Asp Arg Phe Tyr
325 330 335
Val Leu Ile Met Ile Ala Ala Ser Asp Thr Tyr Asn Ser Arg Ile Trp
340 345 350
Trp Thr Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu Ile Ala Ala Glu
355 360 365
Thr Gln Leu Gly Asp Val Tyr Trp Thr Leu Arg His Phe Tyr Glu Trp
370 375 380
Ser Val Arg Pro Ala Tyr Ser Pro Arg Leu Arg Glu Arg Glu Asp Asp
385 390 395 400
Lys Tyr Ile Tyr Gly Arg Val Asn Leu Phe Asp Leu Asp Ala Ser Pro
405 410 415
Gly Thr Lys Ile Ile His Trp Thr Tyr Asp Leu Ile Thr Arg Glu Ser
420 425 430
Lys Ile Thr Tyr His Asn Gly Asn Pro Cys Asp Leu Asn Pro Asp Val
435 440 445
Asp Gly Ile Ile Thr Lys Phe Asn Lys Thr Ala Tyr Thr Asp Met Ile
450 455 460
Gly Glu Leu Leu Asn Gly Gly Trp Asn Thr Arg Glu Phe Lys Met Tyr
465 470 475 480
Lys Ile Leu Glu Ser Val Gly Asn Val Leu Thr Ile Asp Phe Glu Lys
485 490 495
Asp Ala Lys Leu Asn Ser Arg Ser Glu Phe Ile Leu Pro Asp Tyr Tyr
500 505 510
Asp Lys Trp Ile Tyr Ala Pro Met Phe Asn Ala Lys Leu Lys Ile Thr
515 520 525
Glu Val Glu Ile Ala Gln Ala Lys Ser Asp Asp Pro Asp Val Val Arg
530 535 540
Thr Leu Ala Pro Ile Gly Ser Asp Pro Ile Thr Leu Gln Arg Leu Cys
545 550 555 560
Leu Gly Asn Tyr Tyr Asp Ile Arg Pro Ala Met His Gly Arg Ala Leu
565 570 575
Met Lys Lys Gln Glu Gln Thr Ser Tyr His Lys Thr Leu Ala Lys Thr
580 585 590
Lys Asp Tyr Ser Asp Val Leu Asn Arg Arg Gly Tyr Arg Lys Val Glu
595 600 605
Lys Pro Cys Pro Met Val Thr Cys Ser Tyr Val Leu Glu Lys Thr Ala
610 615 620
Leu Phe Trp Ile Asp Val Met Glu Lys His Val Gly Asp Ile Glu Asp
625 630 635 640
Ser Thr Asp Glu Phe Asp Phe Pro Arg Val Asp Val Thr Leu Pro Val
645 650 655
Glu Thr His Lys Val Val Asp Val Ala Gln Leu Ser Val Leu Leu Ile
660 665 670
Asp Phe Leu Tyr Glu Lys Arg Arg Thr Val Arg Asp Ile Asp Ser Ala
675 680 685
Arg Trp Thr Ile Glu Leu Ile Arg Arg Val Arg Gly Lys Asp Arg Leu
690 695 700
Glu Ala Ile Arg Arg Glu Phe Pro Asn Phe Gly Glu Ala Val Thr Ile
705 710 715 720
Leu Met Asn Pro Thr Arg Val Lys Asp Val Met Val Ile Asn Phe Leu
725 730 735
Pro Phe Leu Ile Leu Leu Gly Asp Asn Ile Ser Tyr Glu His Arg Gln
740 745 750
Trp Ser Ile Pro Ile Ile Leu His Ala Asp Ser Met Trp Phe Leu Pro
755 760 765
Val Glu Val Gly Ala Phe Tyr Asn Arg Phe Gly Val Ile Ser Phe Leu
770 775 780
Glu Tyr Met Met Phe Phe Pro Ser Ala Gln Ser Arg Glu Ser Glu Leu
785 790 795 800
Asp Glu Ala Lys Ile Lys Val Ser Lys Leu Ile Leu Glu Phe Tyr Leu
805 810 815
Asp Thr Thr Ile Phe Ser Gly Gly Val Gln Arg Ser Val Val Ser Thr
820 825 830
Lys Gln Ile Leu Tyr Glu Thr Tyr Leu Ser Ser Leu Cys Gly Gly Tyr
835 840 845
Ser Glu Gly Leu Val Trp Tyr Leu Pro Ile Thr His Pro Thr Lys Cys
850 855 860
Val Val Ala Ile Glu Phe Ser Asp Asp Arg Val Asp Ala Ala His Arg
865 870 875 880
Cys Asn Arg Leu Arg Ala Arg Phe Pro Leu Ser Ala Val His Leu Lys
885 890 895
Gly Ile Val Ile Leu Ser Ile Ala Lys Asp Arg Ala Val Lys Ala Tyr
900 905 910
Thr Glu Gly Ile Val Thr His Arg Leu Cys Lys Lys Asn Val Leu Gly
915 920 925
Phe Thr Cys Gln Ile Leu Leu Leu Lys Phe Ser Gly His Val Phe Gly
930 935 940
Asn Asp Glu Met Leu Thr Lys Leu Leu Asn Val
945 950 955
<210> 38
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV14 CAE53030的VP5
<400> 38
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ala
65 70 75 80
Gly Asp Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Met Lys Ile Lys Glu Leu Glu Glu Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys His Gly Lys Glu Ile Thr Lys Lys Phe Gly Glu Glu Leu Glu
115 120 125
Glu Ile Tyr Gln Phe Met Asn Gly Glu Val Lys Asp Glu Asp Glu Gln
130 135 140
Lys Lys Gln Tyr Glu Met Leu Cys Lys Ala Val Asp Ser Tyr Glu Lys
145 150 155 160
Leu Leu Val Ala Glu Asn Asp Glu Met Arg Thr Leu Ala Arg Ala Leu
165 170 175
Arg Arg Glu Thr Thr Glu Arg Thr Glu Val Glu Ser Lys Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Ala Ala Ile Glu Val Gln
195 200 205
Arg Asp Gly Met Gln Glu Glu Gly Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Val Gly Ser
225 230 235 240
Gly Met Ala Thr Ala Ile Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Thr Met Phe Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Arg Asp Ile Pro Asp Lys Glu Leu Ala Val Ser Ile Leu
290 295 300
Ala Lys Asn Asn Ala Ile Val Ala Asn Thr Arg Glu Val Gln His Ile
305 310 315 320
Lys Glu Glu Ile Leu Pro Lys Phe Lys Lys Ile Met Asp Glu Glu Lys
325 330 335
Glu Leu Glu Gly Ile Asp Asp Lys Arg Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Val Pro Arg Ser Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
Phe His Met Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Ala Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Glu Ala Lys Gly Arg Thr Leu Asn Glu Ala Tyr Lys Glu Phe
420 425 430
Leu Asn Leu Ser Ile Gly Ser Thr Tyr Thr Ser Pro Met His Ala Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Thr Leu Lys Ser Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Val
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Lys Asn Ala
515 520 525
<210> 39
<211> 952
<212> PRT
<213> 人工序列
<220>
<223> BTV15 CAE51102的VP2
<400> 39
Met Glu Asp Phe Pro Val Ser Val Ile Glu Arg Asn Ser Thr Ile Ala
1 5 10 15
Pro Ala Ile Ile Lys Arg Tyr Pro Ile Ile Val Asp Val Arg Asn Met
20 25 30
Val Ala Leu Ser Gly Gly Lys Asn Asp Val Thr Asn Ile Pro Arg Val
35 40 45
Arg Asn Ile Gly Val Arg Gln Glu Gly Ile Arg Val Gly Leu Lys Tyr
50 55 60
Arg Pro Asp Thr Glu Thr Asn Val Ile Phe Pro Lys Asn Leu Asp Val
65 70 75 80
Leu Ile Arg Ala Tyr Asp His Lys His Arg Leu Thr Arg Asp Lys Arg
85 90 95
Pro Thr Asp His Asp Val Asp Ser Ile Ser Asp Gly Asp Val Thr Val
100 105 110
Trp Val Thr Asn Arg Thr Asn Glu Phe Tyr Asp Ile Gln Pro Ile Tyr
115 120 125
His Thr Leu Glu Asp Glu Val Cys Lys Val Lys Phe Thr Ser Leu Leu
130 135 140
Gly Ser Val Ser Thr Asp Ser Asn Tyr Ala Glu Ser Met Ser Tyr His
145 150 155 160
Tyr Ile Gly Val Glu Arg Asp Lys Cys Asp His Ser Lys Trp His Arg
165 170 175
Phe Phe Pro Met Leu Leu Ser Asp Ser Ile Asn Leu Gly Lys Glu Val
180 185 190
Gly Tyr Ile Leu Lys Lys Thr Tyr Lys Ile Lys Ala Ile Gly Glu Ala
195 200 205
Arg Glu Gly Gln Arg Gln Glu Leu Arg Val Asp Val Pro Tyr Thr Pro
210 215 220
Gly Ile Gly Arg Asn Glu Arg Ile Ser Asp Met Lys Leu Pro Val Tyr
225 230 235 240
Lys Arg Phe Leu Glu Gly Tyr Ile Arg Cys Gln Ile Glu Pro Asn Val
245 250 255
Pro Glu Lys Leu Thr Glu Leu Lys Asn Gln Leu Asp Ala Ile Cys Val
260 265 270
Ala Trp Tyr Gly Gly Gln Val Pro Ile Val Ala Asp Glu Ile Cys Lys
275 280 285
Ile Thr Ser Arg Ile Gly Arg Leu Met Trp Asn Thr Glu Glu Glu Pro
290 295 300
Val Asp Glu Thr Met Arg Ser Arg Ala Phe Gln Glu Ser Ile Arg Leu
305 310 315 320
Met Phe Arg Thr Glu Asn Ser Glu Tyr Asn Asn Ile Gln Ala Ile Gly
325 330 335
Ser Gly Arg Thr Gln Arg Gln Lys Phe Tyr Ala Pro Leu Ile Ile Ala
340 345 350
Ala Thr Asp Ser Phe Arg Trp Arg Ile Trp Trp Asn Asn Pro Tyr Pro
355 360 365
Cys Leu Arg Gly Cys Leu Ile Ala Cys Glu Met Glu Leu Gly Asp Val
370 375 380
Tyr Lys Ser Leu Lys Ser Ile Tyr Lys Trp Thr Leu Arg Ser Ser Tyr
385 390 395 400
Ser Ser Gln Arg Glu Ile Asp Arg Gln Leu Asn Val Phe Pro Tyr Gln
405 410 415
Lys Ile Asn Leu Phe Asp Phe Ser Gly Thr Pro Gly Thr Glu Ile Ile
420 425 430
His Trp Arg Ile Val His Ile Pro Val Pro Asp Glu Ile Asp Tyr Glu
435 440 445
His Gly Tyr Leu Cys Pro Glu Ser Gly Asp Tyr Asp Ile Val Met Ala
450 455 460
Val Asp Asp Asp Leu Tyr Ser Ala Phe Lys His Arg Val Ile Asp Arg
465 470 475 480
Gly Trp Glu His Gln Ala Met Lys Leu Asp Glu Leu Val Pro Gln Glu
485 490 495
Asn Asn Ile Phe Lys Met Glu Phe Glu Lys Asp Ala Tyr Leu Asp Asn
500 505 510
Arg Asn His Leu Val Leu Pro Pro Tyr Tyr Asn Lys Val Val Tyr Cys
515 520 525
Pro Leu Phe His Ala Thr Ala Lys Ile Thr Pro Thr Glu Ile Ser His
530 535 540
Arg Gln Ser Asp Asp Pro Trp Cys Glu Arg Thr Leu Tyr Gly Phe Arg
545 550 555 560
Gly Asp His Val Ile Thr His Tyr Thr Gly Leu Asn His Ile Val Asn
565 570 575
Arg Arg Lys Pro Leu Met Gly Gln Thr Leu Ser Val Ala Gln Ser Met
580 585 590
Gly Asp Tyr Thr Lys Tyr Ile Leu Gly Asp Asp Gly Ser Ala Asn Ser
595 600 605
Cys Thr Gly Thr Ala Tyr Ser Ser Arg Leu Pro Thr Met Leu Phe Gln
610 615 620
Asp Ile Phe Asp Ala Ile Ile Arg His Leu Pro Lys Val Ile Leu Lys
625 630 635 640
Asp Gln Ala Asp Tyr Phe Ser Glu Ser Arg Lys Arg Val Ser Asp Ile
645 650 655
Ile Asp Trp Asp Thr Phe Ile Val Tyr Ser Ile Tyr Ala Cys Phe Glu
660 665 670
Arg Lys Asn Gln Val Leu Arg Glu Glu Asp Ala Thr Gln Ile Val Arg
675 680 685
Gly Ile Ser Thr Ala Gly Lys Glu Lys Arg Leu Lys Leu Ile Glu Arg
690 695 700
Ser Phe Pro Ile Tyr Tyr Arg Glu Leu Met Lys Ile Lys Asn Ala Lys
705 710 715 720
Thr Trp Lys Asp Ala Tyr Ala Phe Asn Phe Leu Pro Leu Leu Leu Cys
725 730 735
Ala Gly Thr Asn Val Gln Tyr Val His Arg Gln Trp Ser Tyr Pro Val
740 745 750
Leu Val Ser Met Glu Lys Gly Pro Arg Ile Ile Pro Ala Met Val Gly
755 760 765
Arg Asp Leu Ala Asp Gly Asn Leu Gly Asp Trp His Ala Tyr Ile Lys
770 775 780
Tyr Tyr Met Gly Gln His Met Glu Asp Gln Glu Val Glu Glu Asp Ser
785 790 795 800
Leu Glu Ile Leu Lys Ile Ser Leu Glu His Tyr Met Ser His Ser Tyr
805 810 815
Ile Ser Asn Val Lys Asp Val Ala Val Arg Met Thr Lys Glu Ile Val
820 825 830
Leu Asp Leu Tyr Phe Ala Ser Gly Cys Asn Gly Val Ser Glu Met Met
835 840 845
Thr Phe Leu Leu Pro Ile Ile His Pro Arg Lys Gly Leu Val Met Phe
850 855 860
Cys Ile Ala Asp Asp Gln Val Gln Pro Gln Val Gln Cys Glu Thr Ala
865 870 875 880
Leu Arg Arg Phe Pro Tyr Thr Arg Glu Tyr Ile His Asp Val Ile Val
885 890 895
Ile Gln Val Gly Arg Pro Gly Glu Leu Lys Gln Val Trp Lys Arg Gly
900 905 910
Ser Thr Lys Val Lys Val Cys Arg Arg Asn Phe Leu Arg Tyr Asp His
915 920 925
Lys Val Ile Leu Ser Arg Leu Cys Gly Leu Val Tyr Gly Asn Tyr Glu
930 935 940
Leu Met Thr Lys Leu Thr Asn Ile
945 950
<210> 40
<211> 527
<212> PRT
<213> 人工序列
<220>
<223> BTV15 CAE53032的VP5
<400> 40
Met Gly Lys Ile Ile Lys Ser Leu Gly Lys Phe Gly Lys Lys Ile Ser
1 5 10 15
Ser Ala Leu Gly Ser Ser Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Val Glu Ser Asp Leu Gly Ser Ala Thr Ile
35 40 45
Asp Gly Ile Ile Gln Gly Ser Val His Ser Ala Leu Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Ile Leu Asn Val Val Gly Gly
65 70 75 80
Gly Asp Ser Ile Ala Asp Pro Leu Ser Pro Gly Glu Asn Asp Met Val
85 90 95
His Arg Leu Lys Arg Leu Glu Asp Glu Gln Lys Gly Glu Ile Ile Arg
100 105 110
Thr Lys His Asn Ala Gln Val Ile Ala Lys Phe Gly Arg Asp Leu Glu
115 120 125
Asp Val Tyr Lys Phe Ala Ser Arg Glu His Lys Val Gly Glu Glu Glu
130 135 140
Glu Asp Gln Ile Gln Met Leu Glu Lys Ala Leu Arg Ala Tyr Gly Asn
145 150 155 160
Val Val Lys Val Glu Gly Glu Ser Val Gln Lys Leu Ala Lys Ala Leu
165 170 175
Arg Val Asp Glu Glu Glu Arg Thr Asp Glu Glu Thr Arg Met Ile Gly
180 185 190
Glu Tyr Arg Asp Lys Ile Asp Ala Leu Ser Lys Ala Ile Glu Ile Glu
195 200 205
Arg Glu Gly Leu Gln Asp Glu Ala Ile Gln Glu Ile Ala Gly Met Ser
210 215 220
Ala Glu Val Leu Glu Ala Ala Ala Glu Glu Val Pro Ile Phe Gly Ala
225 230 235 240
Gly Val Ala Thr Ser Ile Ala Thr Ala Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Ala Val Ile Thr Ala Leu Thr Gly Ile Asp Leu Ser His
260 265 270
Met Thr Thr Pro Lys Ile Gln Pro Lys Thr Leu Glu Ala Ile Leu Asp
275 280 285
Ala Pro Gly Gly Asn Val Thr Asp Leu Met Leu Thr Arg Gly Leu Asp
290 295 300
Ser Lys Ile Asp Lys Ile Asn Glu Asn Leu Ala Glu Val Glu His Met
305 310 315 320
Gln Thr Ser Ile Leu Pro Arg Ile Lys Lys Ala Ile Glu Glu Asp Arg
325 330 335
Asp Glu Ile Thr Asn Trp Ser Pro Lys Arg Ile His Pro Lys Ser Val
340 345 350
Gln Arg Phe Arg Val Pro Arg Met Gln Thr Pro Ser Ile His Ile Tyr
355 360 365
Ala Ala Pro Trp Asp Ser Asp Ser Val Phe Ile Phe His Val Ile Ser
370 375 380
Pro His His Leu Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Glu Ile
385 390 395 400
Glu Tyr Val His Tyr Glu Asp Leu Ala Gln His Trp His Ser Leu Gly
405 410 415
Ala Ala Gln Glu Val Thr Gly Arg Thr Phe Arg Glu Ala Tyr Arg Glu
420 425 430
Phe Phe Asn Leu Ala Ser Arg Ser Thr Met Ala Ser Asp Ile His Lys
435 440 445
Lys Arg Leu Gln Arg Ser Arg Met Ser His Pro Ile Tyr Leu Gly Val
450 455 460
His Asn Tyr Glu Leu Ser Tyr Ile Ala Ile Lys Ser Asn Ala Met Gln
465 470 475 480
Leu Val Thr Asp Glu Asp Leu Gln Lys His Val Leu Arg Gly Pro Leu
485 490 495
His Phe Gln Arg Arg Val Ile Met Ala Ala Leu Lys Tyr Gly Val Lys
500 505 510
Val Met Ser Arg Ala Asp Asp Ile Ala Met Met Leu Arg Asp Ala
515 520 525
<210> 41
<211> 959
<212> PRT
<213> 人工序列
<220>
<223> BTV16 CAE51103的VP2
<400> 41
Met Glu Glu Leu Val Ile Pro Val Ile Thr Arg Gln Phe Asp Lys Lys
1 5 10 15
Leu Val Gly Arg Tyr Asp Tyr Val Ile Glu Leu Thr Glu Pro Glu Asp
20 25 30
Gly Glu Trp Ser Gly His Asp Val Thr Glu Ile Pro Asn Arg Arg Met
35 40 45
Phe Asp Ile Lys Gln Gln Asn Ile Arg Glu Ala Ile Glu Tyr Lys Pro
50 55 60
Val Asp Asn Asp Gly Glu Val Leu Pro Arg Ile Leu Asp Met Ser Ile
65 70 75 80
Ala Cys Tyr Asp Met Lys Lys Ser Met Met Lys Lys Asp Gly Val Asp
85 90 95
Phe Val Ser Asn Thr Arg Trp Leu Glu Trp Met Ile Gly Asp Ser Met
100 105 110
Asp Val Gln Pro Leu Arg Val Gln Leu Lys Glu Asp His Ser Thr Ile
115 120 125
Gln Tyr Gly Met Phe Ser Asn Thr Leu His Ile Asp Ser Arg Lys Ala
130 135 140
Asp Thr Thr Ser Tyr His Thr Ile Ala Val Glu Ser Lys Gly Glu Arg
145 150 155 160
Gly Cys Cys His Val His Thr Ala Ile Trp Asn His Met Val Arg Asn
165 170 175
His Leu Phe Asn Ala Val Gln Glu Ala Cys Tyr Val Phe Lys Pro Thr
180 185 190
Tyr Asp Leu Ile Val Ile Gly Glu Lys Gln Asn Arg Glu Asp Glu Phe
195 200 205
Arg Ile Gly Glu His Asn Phe Tyr Thr Ile Thr Arg Asn His His Met
210 215 220
Arg Leu Gly Asp Asn Ala Tyr Asn Gln Phe Met Lys Gly Leu Val Gln
225 230 235 240
Leu Arg Val Ala Gly Val Thr Pro Asn Ala Ile Arg Glu Glu Met Ala
245 250 255
Ala Leu Asp Ala Ile Arg Asp Thr Trp Ile Gly Gly Asn Phe Glu Arg
260 265 270
Thr His Ile Lys Ser Leu Glu Ile Cys Lys Leu Leu Ser Ser Ile Gly
275 280 285
Arg Lys Met Val Asn Met Glu Glu Glu Pro Lys Asp Glu Arg Asp Leu
290 295 300
Ser Val Lys Phe Gln Phe Lys Leu Asp Asp Lys Phe Ser Thr Thr Asp
305 310 315 320
Pro Glu Arg Asn Val Ile Phe Thr His Lys Thr His Arg Thr Asn Gln
325 330 335
Asp Arg Phe Tyr Val Ser Leu Met Ile Ala Ala Ser Asp Thr Asn Asn
340 345 350
Gly Arg Val Trp Trp Ser Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu
355 360 365
Ile Ala Ser Glu Cys Lys Leu Gly Asp Val Tyr His Lys Leu Arg Ala
370 375 380
Trp Tyr Glu Trp Ser Val Arg Pro Glu Tyr Lys Pro Arg Asp Leu Glu
385 390 395 400
Arg Glu Gln Glu Lys Tyr Ile Val Gly Arg Val Asn Leu Phe Asp Leu
405 410 415
Glu Gly Glu Pro Ala Thr Lys Val Phe His Trp Glu Tyr Glu Leu Ile
420 425 430
Asn Lys Val Tyr Gln Ile Thr Asn His Thr Gly Asn His Cys Asp Leu
435 440 445
Tyr Pro Asp Asp Val Glu Ile Thr Ala Lys Phe Asp Glu Glu Lys Tyr
450 455 460
Gly Glu Met Ile Gln Met Ile Ile Asn Glu Gly Trp Lys His Gly Asp
465 470 475 480
Phe Lys Met Phe Lys Ile Leu Lys Glu Glu Gly Asn Pro Leu Leu Tyr
485 490 495
Asp Leu Glu Lys Asp Ile Arg Leu Asp Ser Arg Ser Gln Val Ile Phe
500 505 510
Pro Pro Tyr Phe Asn Lys Trp Thr His Ala Pro Met Phe Asn Ala Lys
515 520 525
Val Lys Pro Cys Glu Val Glu Leu Ala Gln Arg Lys Asn Glu Asp Pro
530 535 540
Tyr Val Lys Arg Thr Val Lys Pro Ile Arg Ala Asp Cys Ile Asp Leu
545 550 555 560
Leu Arg Tyr His Met Ser His Tyr Met Asp Val Arg Val Ser Met Lys
565 570 575
Gly Leu Ser Leu Ala Val Lys Gln Thr Pro Ser Ser Ile His Gln Glu
580 585 590
Trp Ala Lys Asp Pro Leu Tyr Pro Gly Phe Leu Gln Arg Arg Asp Glu
595 600 605
Asn Leu Asp His Lys Ser Val Cys Pro Ile Val Thr Asn Tyr Phe Trp
610 615 620
Leu Glu Arg Phe Tyr Thr Leu Val Leu Thr Ile Met Glu Lys His Tyr
625 630 635 640
Trp Asp Leu Asp Asp Ser Asp Val Thr Tyr Glu Phe Pro Thr Leu Asp
645 650 655
Asp Ser Ala Tyr Lys Ile Glu Gly Thr Leu Tyr Asp Val Ser Gln Val
660 665 670
Val Val His Met Ile Asp Arg Phe Phe Glu Lys Arg Arg Phe Leu Arg
675 680 685
Ser Val Asp Glu Cys Arg Trp Ile Leu His Leu Ile Arg Ser Thr Arg
690 695 700
Ser Arg Asp Arg Leu Ser Val Phe Lys Arg Phe Phe Pro Thr Phe Gly
705 710 715 720
Glu Gly Leu Cys Val Asn Asn Phe Arg Lys Val Lys Asp Ile Met Leu
725 730 735
Leu Asn Phe Leu Pro Phe Phe Phe Leu Ile Gly Asp Asn Met Ala Tyr
740 745 750
Glu His Arg Gln Trp Ala Val Pro Leu Leu Phe Tyr Ala Asp Lys Ile
755 760 765
Arg Val Ile Pro Ala Glu Val Gly Ala Tyr Tyr Asn Arg Phe Gly Leu
770 775 780
Val Ser Ile Leu Glu Leu Leu Thr Phe Phe Pro Ser Tyr Glu Met Arg
785 790 795 800
Asp Asn Lys Val Glu Glu Asp Val His Ala Cys Ala Ser Ala Ile Leu
805 810 815
Asp Phe Tyr Leu Thr Thr Thr Ile Ser Asn Gly Gly Ile Gln Ala Ser
820 825 830
Ile Val Ser Thr Lys Ala Leu Leu Tyr Glu Thr Tyr Leu Ser Ser Val
835 840 845
Cys Gly Gly Phe Ser Glu Ala Ile Leu Trp Tyr Leu Pro Ile Thr His
850 855 860
Pro Val Lys Cys Leu Val Ala Leu Glu Val Ser Asp Ser Leu Val Asp
865 870 875 880
Ser Asn Val Arg Ile Asp Lys Ile Lys Arg Arg Phe Pro Leu Ser His
885 890 895
Arg His Leu Arg Gly Ile Val Gln Ile Ser Val Arg Pro Asp Arg Thr
900 905 910
Phe Gly Val Thr Thr Cys Gly Ile Val Lys His Lys Ile Cys Lys Lys
915 920 925
Thr Leu Leu Lys Gln Arg Cys Asp Val Ile Leu Ile Gln Ile Pro Gly
930 935 940
Tyr Val Phe Gly Asn Asp Glu Leu Leu Thr Lys Leu Leu Asn Ile
945 950 955
<210> 42
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV16 CAE53035的VP5
<400> 42
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ala
65 70 75 80
Gly Asp Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Met Lys Ile Lys Glu Ile Glu Glu Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys His Gly Lys Glu Ile Thr Lys Lys Phe Gly Glu Glu Leu Glu
115 120 125
Glu Ile Tyr Gln Phe Met Asn Gly Glu Val Lys Asp Glu Glu Glu Gln
130 135 140
Glu Glu Gln Tyr Lys Val Leu Cys Lys Ala Val Asn Ser Tyr Glu Lys
145 150 155 160
Leu Leu Ile Ala Glu Asn Asp Lys Met His Ile Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Ala Glu Arg Thr Glu Ala Glu Ser Thr Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Ala Ala Ile Glu Ile Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Val Gly Ser
225 230 235 240
Gly Met Ala Thr Ala Ile Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Thr Met Val Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Glu Asp Ile Pro Asp Lys Lys Leu Ala Met Ser Ile Leu
290 295 300
Ala Lys Asn Asn Ala Ile Thr Ala Asn Thr Arg Glu Ile Gln His Ile
305 310 315 320
Lys Glu Glu Ile Leu Pro Lys Phe Lys Lys Val Met Asp Glu Glu Lys
325 330 335
Glu Leu Glu Gly Ile Asp Asp Lys Lys Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Val Pro Arg Ser Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Ile Ser His
370 375 380
Phe His Ala Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Ala Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Glu Ala Lys Gly Arg Thr Leu Asn Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ala Ile Gly Ser Thr Tyr Thr Ser Pro Met His Ala Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Asp Thr Leu Lys Ser Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Val
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Lys Asn Ala
515 520 525
<210> 43
<211> 955
<212> PRT
<213> 人工序列
<220>
<223> BTV17 CAE51104的Vp2
<400> 43
Met Glu Glu Phe Val Ile Pro Val Tyr Ser Glu Asp Glu Ile Pro Tyr
1 5 10 15
Ala Leu Leu Ser Arg Tyr Pro Leu Ala Ile Gln Thr Asn Val Lys Ile
20 25 30
Glu Asp Val Glu Gly Lys His Asn Val Val Lys Ile Pro Glu Ser Asp
35 40 45
Met Ile Asp Ile Pro Lys Leu Thr Ile Val Glu Ala Met Asn Tyr Lys
50 55 60
Pro Ala Arg Asn Asp Gly Ile Val Val Pro Arg Leu Leu Asp Ile Thr
65 70 75 80
Leu Arg Ala Tyr Asp Asp Arg Lys Ser Thr Lys Ser Ala Arg Gly Ile
85 90 95
Glu Phe Met Thr Asn Ala Arg Trp Met Lys Trp Ala Ile Asp Asp Arg
100 105 110
Met Asp Ile Gln Pro Leu Lys Val Thr Leu Asp His Tyr Cys Ser Val
115 120 125
Asn His Gln Leu Phe Asn Cys Val Val Lys Ala Asn Ala Ala Asn Ala
130 135 140
Asp Thr Ile Tyr Tyr Asp Tyr Phe Pro Leu Glu Asp Tyr Lys Lys Arg
145 150 155 160
Cys Asn His Thr Asn Leu Asp Leu Leu Arg Ser Leu Thr Asn Met Glu
165 170 175
Leu Phe His Ala Leu Gln Gly Ala Ala Tyr Ser Ile Lys Ser Ser Tyr
180 185 190
Glu Leu Val Ala Tyr Ser Glu Arg Gly Ser Leu Glu Glu Thr Tyr Val
195 200 205
Val Gly Gln Pro Lys Trp Ile His Leu Thr Arg Gly Thr Arg Ile Gly
210 215 220
Asn Ser Gly Leu Ser Tyr Glu Arg Phe Ile Ser Ser Met Val Gln Val
225 230 235 240
Ser Val Asn Gly Lys Ile Pro Asp Glu Ile Ala Asn Glu Ile Ala Gln
245 250 255
Leu Asn Arg Ile Arg Ala Glu Trp Ile Thr Ala Thr Tyr Asp Arg Gly
260 265 270
Arg Ile Arg Ala Leu Glu Leu Cys Asn Ile Leu Ser Thr Ile Gly Arg
275 280 285
Lys Met Leu Asn Thr His Glu Glu Pro Lys Asp Glu Met Asp Leu Ser
290 295 300
Thr Arg Phe Gln Phe Lys Leu Asp Glu Lys Phe Asn Arg Ala Asp Ser
305 310 315 320
Glu His Val Asn Ile Phe Gly Val Arg Gly Pro Ala Thr Asp Glu Gly
325 330 335
Arg Phe Tyr Ala Leu Ile Ala Ile Ala Ala Thr Asp Thr Gln Lys Gly
340 345 350
Arg Val Trp Arg Thr Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu Val
355 360 365
Ala Ala Glu Cys Glu Leu Gly Asp Val Tyr Ser Thr Leu Arg Arg Val
370 375 380
Tyr Arg Trp Ser Leu Arg Pro Glu Tyr Gly Gln His Glu Arg Gln Leu
385 390 395 400
Glu Asn Asn Lys Tyr Val Phe Asn Arg Ile Asn Leu Phe Asp Ser Asn
405 410 415
Leu Ala Val Gly Asp Gln Ile Ile His Trp Arg Tyr Glu Val Lys Ala
420 425 430
Ser Ala Glu Thr Thr Tyr Asp Ser Gly Tyr Met Cys Arg His Glu Ala
435 440 445
Glu Glu Asp Glu Leu Leu Cys Lys Ile Asn Glu Asp Lys Tyr Lys Glu
450 455 460
Met Leu Asp Arg Met Ile Gln Gly Gly Trp Asp Gln Glu Arg Phe Lys
465 470 475 480
Leu His Asn Ile Leu Thr Asp Pro Asn Leu Leu Thr Ile Asp Phe Glu
485 490 495
Lys Asp Ala Tyr Leu Asn Ser Arg Ser Glu Leu Val Leu Pro Asp Tyr
500 505 510
Phe Asp Lys Trp Ile Ser Ser Pro Met Phe Asn Ala Arg Leu Arg Ile
515 520 525
Thr Lys Gly Glu Ile Gly Thr Ser Lys Lys Asp Asp Pro Trp Asn Asn
530 535 540
Arg Ala Val Arg Gly Tyr Ile Lys Ser Pro Ala Glu Ser Leu Asp Phe
545 550 555 560
Val Leu Gly Pro Tyr Tyr Asp Leu Arg Leu Leu Phe Phe Gly Glu Thr
565 570 575
Leu Ser Leu Lys Gln Glu Gln Ser Ala Val Phe Gln Tyr Leu Ser Gln
580 585 590
Leu Asp Asp Phe Pro Ala Leu Thr Gln Leu Thr Gly Asp Ala Val Cys
595 600 605
Pro His Ser Gly Gly Ala Leu Tyr Thr Phe Arg Lys Val Ala Leu Phe
610 615 620
Leu Ile Gly Asn Tyr Glu Lys Leu Ser Pro Asp Leu His Glu Gly Met
625 630 635 640
Glu His Gln Arg Tyr Val His Pro Ser Thr Gly Gly Thr Tyr Gln Lys
645 650 655
Arg Val Leu Glu Met Lys Asp Pro Cys Gln Leu Thr Cys Phe Val Ile
660 665 670
Asp Tyr Ile Phe Glu Lys Arg Glu Gln Leu Arg Asp Thr Lys Glu Ala
675 680 685
Arg Tyr Ile Val Tyr Leu Ile Gln Ser Leu Thr Gly Thr Gln Arg Leu
690 695 700
Ser Val Leu Arg Ser Thr Phe Pro Asn Phe Phe Gln Arg Leu Leu Met
705 710 715 720
Leu Lys Glu Ile Lys Phe Val Arg Asp Leu Asn Val Ile Asn Phe Leu
725 730 735
Pro Leu Met Phe Leu Val His Asp Asn Ile Ser Tyr Trp His Arg Gln
740 745 750
Trp Ser Ile Pro Met Val Leu Phe Asp Asp Thr Ile Lys Leu Ile Pro
755 760 765
Val Glu Val Gly Ala Tyr Ala Asn Arg Phe Gly Phe Lys Ser Phe Met
770 775 780
Asn Phe Thr Arg Phe His Pro Gly Glu Leu Lys Lys Lys Gln Ile Ala
785 790 795 800
Glu Asp Ile His Lys Glu Phe Gly Val Val Ala Phe Glu Tyr Tyr Thr
805 810 815
Asn Thr Lys Ile Ser Gln Gly Asn Val His Thr Pro Val Met Thr Thr
820 825 830
Lys Met Asp Val Leu Arg Val His Leu Ser Ser Leu Cys Ala Gly Leu
835 840 845
Ala Asp Ser Val Val Tyr Thr Leu Pro Val Ala His Pro Lys Lys Cys
850 855 860
Ile Val Leu Ile Ile Val Gly Asp Asp Lys Leu Glu Pro His Thr Arg
865 870 875 880
Ser Glu Gln Ile Val Ser Arg Tyr Asn Tyr Ser Arg Lys His Ile Cys
885 890 895
Gly Ile Val Ser Val Thr Ile Gly Gln Asn Ser Gln Leu Arg Val His
900 905 910
Thr Ser Gly Ile Val Lys His Arg Val Cys Asp Lys Phe Ile Leu Lys
915 920 925
His Lys Cys Lys Val Ile Leu Val Arg Met Pro Gly Tyr Val Phe Gly
930 935 940
Asn Asp Glu Leu Met Thr Lys Leu Leu Asn Val
945 950 955
<210> 44
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV17 CAE53036的VP5
<400> 44
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ala Ala Thr Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Thr
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Thr Lys Ile Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Lys Glu Ile Thr Lys Glu Phe Gly Lys Glu Leu Glu
115 120 125
Glu Val Tyr Asp Phe Met Asn Gly Glu Ala Lys Glu Glu Glu Val Val
130 135 140
Gln Glu Gln Tyr Ser Met Leu Cys Lys Ala Val Asp Ser Tyr Glu Lys
145 150 155 160
Ile Leu Lys Ala Glu Asp Ser Lys Met Ala Met Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Ser Glu Arg Ser Gln Asp Glu Ile Lys Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Asn Ala Ile Glu Ile Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Met Arg Ser Pro Lys Ile Glu Pro Thr Ile Ile Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Lys Glu Ile Pro Asp Glu Gln Leu Ala Val Ser Val Leu
290 295 300
Asn Lys Lys Thr Ala Val Thr Asp Asn Cys Asn Glu Ile Ala His Ile
305 310 315 320
Lys Gln Glu Ile Leu Pro Lys Phe Lys Gln Ile Met Asp Glu Glu Lys
325 330 335
Glu Ile Glu Gly Ile Glu Asp Lys Val Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Ile Pro Arg Thr Gln Gln Pro Gln Ile His Ile Tyr Ala
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Arg Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Thr Ser His Trp His Ala Leu Gly Leu
405 410 415
Ala Gln Glu Ala Ser Gly Arg Thr Leu Thr Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ser Ile Ser Ser Thr Tyr Ser Ser Ala Ile His Ala Arg
435 440 445
Arg Met Ile Arg Ser Arg Ala Val His Pro Ile Phe Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Ala Leu Lys Asn Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Glu Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Ile
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 45
<211> 957
<212> PRT
<213> 人工序列
<220>
<223> BTV18 CAE51105的VP2
<400> 45
Met Asp Glu Leu Ala Ile Pro Ile Tyr Thr Asp Thr Phe Pro Ala Gly
1 5 10 15
Leu Leu Asp Gly Tyr Asp Tyr Ile Ile Asp Val Ser Thr Arg Val Asp
20 25 30
Glu Pro Lys Gly Arg His Asp Val Thr Glu Ile Pro Lys Arg Ser Met
35 40 45
Phe Asp Ile Lys Asp Glu Pro Ile Arg Glu Ala Ile Ile Tyr Lys Pro
50 55 60
Val Ser Asn Asp Gly Cys Val Leu Pro Arg Val Leu Asp Ile Thr Leu
65 70 75 80
Arg Ala Phe Asp Asp Arg Lys Arg Val Ile Thr Asn Glu Gly His Ser
85 90 95
Glu Phe His Thr Arg Ser Asn Trp Leu Gln Trp Met Ile Asp Asp Ala
100 105 110
Met Asp Tyr Gln Pro Leu Lys Val Asp Val Glu His Val Pro Ser Arg
115 120 125
Ile Ser His Ala Leu Ser Asn Ser Thr Val Arg Leu His Ala Lys Arg
130 135 140
Ala Asp Thr Ala Ser Tyr His Ile Glu Pro Val Glu Val Glu Ala Tyr
145 150 155 160
Gly Cys Asn His Val Trp Thr Glu Arg Ile His His Leu Val Asn Ile
165 170 175
Glu Met Phe His Ser Ser Gln Glu Ala Ala Tyr Ile Leu Lys Pro Thr
180 185 190
Tyr Gln Ile Ile Ala His Ala Glu Arg Ala Ala Val Gly Asp Ala Phe
195 200 205
Asn Gly Arg Thr Leu Glu Leu Asn Arg Asn His Arg Ile Gln Leu Gly
210 215 220
Asp Gln Gly Tyr Val Lys Leu Lys Lys Gly Trp Val Gln Thr Arg Ile
225 230 235 240
Glu Gly Lys Lys Pro Ala Ala Ile Glu Arg Glu Ile Ala Ser Leu Asn
245 250 255
Glu Ile Arg Ala Gln Trp Ile Ala Arg Gly His Asp Arg Arg Glu Ile
260 265 270
Lys Ala Leu Asp Leu Cys Arg Ile Leu Ser Ala Ile Gly Arg Lys Met
275 280 285
Tyr Asn Ile Glu Glu Glu Pro Lys Asn Glu Ala Asp Met Ser Val Lys
290 295 300
Phe Gln Met Glu Leu Asp Glu Ile Phe Lys Pro Gly Asn Ser Glu His
305 310 315 320
Asp Asn Ile Met Gly Gly Gly Thr His Arg Lys Asp Glu Asp Arg Phe
325 330 335
Tyr Val Leu Ile Val Ile Ala Ala Ser Asp Thr Asn Gln Gly Arg Val
340 345 350
Trp Trp Ser Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu Ile Ala Ala
355 360 365
Glu Val Gln Leu Gly Asp Val Tyr Ser Thr Leu Arg Ser Trp Tyr Gln
370 375 380
Trp Ser Ile Arg Pro Ala Tyr Val Pro Tyr Asp Arg Ser Arg Glu Asn
385 390 395 400
Asp Lys Tyr Ile Tyr Thr Arg Thr Asn Leu Phe Asp Ser Ala Leu Gly
405 410 415
Pro Gly Asp Lys Ile Ile His Trp Glu Tyr Gln Leu Leu Asn Glu Thr
420 425 430
Arg Glu Val Ser Met Ser Ser Gly Asn Glu Cys Asp Leu Phe Pro Glu
435 440 445
Asp Glu Glu Phe Thr Thr Lys Phe Asn Glu Glu Arg Tyr Thr Glu Met
450 455 460
Lys Gly Gln Ile Ile Gln Ser Gly Trp Asn His Arg Asp Phe Lys Met
465 470 475 480
His Lys Ile Leu Glu Asp Gly Ala Asn Val Leu Thr Ile Asp Phe Glu
485 490 495
Lys Asp Ala Arg Val Gly Thr Gly Ser Val Leu Thr Leu Pro Asp Tyr
500 505 510
Tyr Asp Lys Trp Ile Val Ala Pro Met Phe Asn Ala Lys Leu Arg Ile
515 520 525
Thr Glu Thr Val Ile Ala Asn Ala His Ser Asp Asp Pro Ala Ile Glu
530 535 540
Arg Ser Val Lys Pro Phe Asp His Asp Pro Ser Thr Leu Gln Arg Phe
545 550 555 560
Cys Leu Ala Arg Tyr Tyr Asp Ile Arg Pro Gly Leu Arg Gly Arg Ala
565 570 575
Leu Leu Lys Gln Gln Asp Gly Ser Thr Ile Thr Lys Lys Val Ser Ala
580 585 590
Leu Asp Asp Tyr Gln Asp Val Val Ser Arg Arg Val Asn Tyr Val Gln
595 600 605
Arg Asp Val Arg Cys Pro Thr Glu Thr Ala Gln Tyr Val Leu Glu Lys
610 615 620
Thr Ser Leu Tyr Leu Leu Ala Leu Phe Asp Lys His Val Met Pro Ser
625 630 635 640
Ala Glu Ser Glu Ala Lys Phe Val His Pro Ser Ile Asp Val Lys His
645 650 655
Asp Ile Glu Thr Trp Arg Ile Ala Asp Ile Ser Gln Leu Ile Val Leu
660 665 670
Ile Phe Asp Tyr Leu Phe Glu Ser Arg Lys Val Val Arg Asp Ala Val
675 680 685
Glu Ala Arg Trp Ile Leu Tyr Lys Ile Arg Ser Ser Thr Gly Arg Ala
690 695 700
Arg Met Glu Val Ile Glu Thr Thr Phe Pro Arg Phe Gly Leu Thr Val
705 710 715 720
Lys Asn Ile Thr Lys Ala Lys Val Asn Gln Asp Ile Ala Cys Leu Asn
725 730 735
Phe Phe Pro Leu Phe Phe Ile Val Gly Asp Asn Met Ala Tyr Ala His
740 745 750
Arg Gln Trp Ser Ile Pro Ile Leu Leu Tyr Ala His Asp Ile Arg Ile
755 760 765
Ile Pro Leu Glu Val Gly Ala Tyr Ser Asn Arg Phe Gly Leu Thr Ser
770 775 780
Tyr Leu Glu Tyr Met Ile Phe Phe Pro Ser Tyr Ala Thr Arg Met Ala
785 790 795 800
Met Ile Asp Glu Ala Asn Lys Glu Cys Ala Ala Ala Leu Leu Asp Tyr
805 810 815
Tyr Leu Ser Thr Asp Val His Arg Gly Gly Val Met Thr Ser Val Ile
820 825 830
Thr Thr Lys Arg Leu Leu Tyr Glu Thr Tyr Leu Ser Ser Leu Cys Gly
835 840 845
Gly Tyr Ser Asp Gly Leu Leu Trp Tyr Ile Pro Ile Thr His Pro Leu
850 855 860
Lys Cys Leu Val Ala Phe Glu Val Ala Asp Asp Val Val Pro Ala Thr
865 870 875 880
Val Arg Lys Glu Arg Ile Leu Met Arg Phe Pro Leu Ser Ala Arg His
885 890 895
Leu Arg Gly Ile Ala Leu Ile Thr Ile Asp Arg Asp Arg Lys Val His
900 905 910
Val Gln Thr Glu Gly Ile Val Ser His Arg Leu Cys Lys Lys Asn Leu
915 920 925
Leu Lys Tyr Val Cys Asp Val Ile Leu Phe Lys Phe Ser Gly Tyr Val
930 935 940
Phe Gly Asn Asp Glu Met Leu Thr Lys Leu Leu Asn Val
945 950 955
<210> 46
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV18 CAE53037的VP5
<400> 46
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Leu Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Met Gly Ser
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Met Gln
85 90 95
Thr Lys Ile Arg Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Ile Arg
100 105 110
Leu Lys Tyr Asn Asp Lys Ile Lys Gln Lys Phe Gly Lys Glu Leu Glu
115 120 125
Glu Val Tyr Glu Phe Met Asn Gly Val Ala Lys Gln Glu Glu Asp Glu
130 135 140
Glu Lys His Tyr Asp Val Leu Lys Lys Ala Val Asn Ser Tyr Asp Lys
145 150 155 160
Ile Leu Thr Glu Glu Glu Lys Gln Met Arg Ile Leu Ala Thr Ala Leu
165 170 175
Gln Lys Glu Val Lys Glu Arg Thr Gly Thr Glu Ala Val Met Val Lys
180 185 190
Glu Tyr Arg Asn Lys Ile Asp Ala Leu Lys Glu Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Thr His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Thr Ile Val Ser Thr Val Leu Asp
275 280 285
His Lys Phe Lys Asp Ile Pro Asp Glu Met Leu Ala Val Ser Val Leu
290 295 300
Ser Lys Asn Arg Ala Ile Glu Glu Asn His Lys Glu Ile Ile His Leu
305 310 315 320
Lys Asn Glu Ile Leu Pro Arg Phe Lys Lys Ala Met Asp Glu Glu Lys
325 330 335
Glu Ile Cys Gly Ile Glu Asp Lys Lys Ile His Pro Lys Val Met Met
340 345 350
Lys Phe Lys Ile Pro Arg Thr Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Ile Ser His
370 375 380
His His Ala Asn Glu Ser Phe Phe Ile Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Leu Val His Tyr Glu Asp Leu Thr Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Ala Ala Val Gly Arg Ser Leu Asn Glu Val Tyr Lys Glu Phe
420 425 430
Leu Asn Leu Ala Ile Asn Asn Thr Tyr Ser Ser Gln Met His Ala Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Leu
450 455 460
His Tyr Asp Ile Ser Phe Ser Thr Leu Arg Ser Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Glu Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Ile Lys His Gly Val Lys Ile
500 505 510
Leu Gly Thr Glu Val Asp Ile Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 47
<211> 962
<212> PRT
<213> 人工序列
<220>
<223> BTV19 CAE51106的VP2
<400> 47
Met Asp Glu Phe Ala Val Pro Ile Val Arg Gly Arg Thr Ile Pro Tyr
1 5 10 15
Asp Leu Ile Arg Ala Tyr Glu Thr Ile Ile Asp Leu Asp Val Lys Val
20 25 30
Glu Glu Asn Glu Gly Lys His Asp Ile Thr Lys Ile Pro Glu Ser Tyr
35 40 45
Met Cys Asp Val Pro Asp Ile Pro Ile Gln Asp Ala Thr Thr Ala Thr
50 55 60
Leu Ser Arg Asn Asp Gly Tyr Ala Leu Pro Arg Ile Leu Asp Val Ser
65 70 75 80
Ile Ala Ala Tyr Asp Thr Arg Lys Gly Tyr Gln Lys Tyr Asn Gly Ile
85 90 95
Glu Arg Met Thr Asn Gln Val Trp Leu Lys Trp Ala Ile Lys Asp Phe
100 105 110
Met Asp Val Gln Pro Leu Lys Val Ser Leu Thr Asp Ala Glu Gly Lys
115 120 125
Val Arg Tyr Asp Leu Phe Asn Cys Ser Val Arg Met Asp Ala Lys Lys
130 135 140
Ala Glu Ala Val Tyr Tyr Gly Trp Ala Ala Gln Glu Val Ile Asp Lys
145 150 155 160
Gly Cys Asn His Ala Arg Ser Glu Leu Leu Gln His Val Ile Arg Cys
165 170 175
Glu Leu Ser Asn Cys Ala Gln Glu Ser Ala Tyr Met Leu Lys Pro Thr
180 185 190
Gln Lys Leu Glu Pro Val Ala Glu Lys Thr Ser Leu Ser Glu Arg Leu
195 200 205
Leu Pro Gly Ser Ser His Leu Thr Ser Met Arg Arg Gly Glu Val Phe
210 215 220
Arg Met Gly Thr Glu Gln Tyr Asn Arg Met Val Gln Gly Met Val Ser
225 230 235 240
Ser Gln Gly Val Lys Gly His Thr Pro Gly Lys Thr Arg Ile Gln Asp
245 250 255
Glu Met Asn Glu Leu Met Lys Ile Arg Asp Arg Trp Lys Val Ala Arg
260 265 270
Asp Arg Arg Glu Ile Lys Ser Leu Glu Leu Cys Lys Leu Leu Ser Ser
275 280 285
Val Gly Arg Lys Met Tyr Asp Lys Tyr Glu Glu Pro Lys Asp Glu Glu
290 295 300
Ala Met Ser Leu Arg Phe Gln Trp Asn Leu Asp Glu Ile Phe Lys Thr
305 310 315 320
Asp Asn Arg Glu His Ile Asn Ile Ala Val Glu Lys Ser Gly Arg Thr
325 330 335
Asn Asp Glu Arg Phe Phe Ala Leu Ile Met Ile Ala Ala Thr Asp Thr
340 345 350
Asn Arg Gly Arg Ile Trp Trp Ser Asn Met Phe Pro Cys Leu Arg Gly
355 360 365
Ala Leu Ile Val Gly Glu Thr Gln Ser Gly Asp Val Tyr Lys Met Leu
370 375 380
Arg Val Arg Phe Asp Trp Ser Val Arg Asn Glu Tyr Ser Thr Gln Ala
385 390 395 400
Asp Val Ala Arg Gln Asn Asp Asn Tyr Thr Phe Thr Arg Val Asn Leu
405 410 415
Phe Asp Leu Ile Ser Glu Pro Ala Thr Pro Val Ile His Trp Glu Tyr
420 425 430
Asp Leu Asp Ala Pro Arg Lys Thr Asn Tyr Leu Asp Gly His Ala Cys
435 440 445
Asp Glu Phe Pro Asp Asp Tyr Asp Leu Val Cys Ser Phe Asp Glu Thr
450 455 460
Lys Tyr Ser Glu Met Ile Gln Arg Ile Leu Asp Gly Gly Trp Asp His
465 470 475 480
Lys Asn Phe Lys Tyr Tyr Lys Ile Leu Lys Asp Ala Gly Asn Val Leu
485 490 495
Thr Ile Asp Phe Glu Lys Asp Ala Arg Leu Asn Glu His Ser Glu Met
500 505 510
Val Met Pro Ser Tyr Phe Asn Lys Arg Ile Ala Ala Pro Met Phe Arg
515 520 525
Cys Ile Leu Arg Ile Cys Glu Thr Thr Ile Ser Val Arg Gln Asn Ala
530 535 540
Asp Pro Ala Val Ser Arg Thr Ile Arg Pro Leu Lys Ala Lys Ala Ala
545 550 555 560
Asp Leu Leu Asp Phe Thr Leu Gly Ser Tyr Tyr Asp Thr Arg Phe Gly
565 570 575
Val Met Gly Arg Thr Leu Val Met Ser Gln Lys Gln Ser Glu Met Tyr
580 585 590
Lys Met Ile Gln Glu Ala Glu Asp Tyr Ser Glu Leu Met Lys Ser Arg
595 600 605
Gly Val Ile Glu Lys Phe Lys Asn Pro Cys Pro Ile Thr Thr Ala Cys
610 615 620
Gln Tyr Thr Leu Gln Lys Thr Ser Val Leu Ile Leu Ser Arg Ala Gln
625 630 635 640
Arg Tyr Phe Arg Glu Phe His Gly Gly Asp Gln Glu Glu Tyr Met Ser
645 650 655
Pro Thr Leu Asn Phe Glu Trp Ser Asp Met Thr Ser Asn Ile Tyr Asp
660 665 670
Val Ala Gln Met Val Val Leu Val Phe Asp Val Leu Phe Glu Arg Arg
675 680 685
Arg Val Val Arg Gly Val Asp Glu Ser Arg Phe Ile Leu His Gln Leu
690 695 700
Arg Ile Arg Lys Gly Leu Asn Arg Leu Asp Tyr Val Lys Ile His Phe
705 710 715 720
Pro His Leu Tyr Gln Trp Trp Lys Ala Ser Glu Ser Thr Tyr Gly Glu
725 730 735
Ala Gln Lys Leu Asn Leu Leu Pro Leu Leu Phe Leu Val Gly Asp Asn
740 745 750
Phe Ile Tyr Glu His Arg Gln Trp Ala Tyr Pro Ala Val Leu Ile Ser
755 760 765
His Lys Leu Arg Phe Ile Pro Val Glu Leu Gly Ala Tyr Tyr Asn Arg
770 775 780
Phe Gly Leu Thr Ser Leu Ile Ser His Leu Thr Phe His Val Gly Lys
785 790 795 800
Ala Lys Tyr Asp Gln Asn Leu Asp Gln Ala Glu Gln Lys Val Phe Ser
805 810 815
Ala Leu Lys Asp Tyr Tyr Ala Glu Ser Thr Ile Thr Asp Gly Gly Val
820 825 830
Asn Thr His Ser Thr Met Thr Lys Asp Pro Ile Tyr Gln Val Tyr Leu
835 840 845
Ser Thr Ile Cys Gly Gly Leu Leu Asp Ser Leu Val Tyr Leu Met Pro
850 855 860
Ile Thr His Pro Ile Lys Ser Ile Val Ala Ile Val Ile Asn Asp Gly
865 870 875 880
Lys Gly Ser Ala Ser Asp Cys Val Arg Arg Val Thr Arg Lys Leu Arg
885 890 895
Tyr Ile Gln His His Ile Arg Gly Ser Val Val Ile Ser Ile Leu Ser
900 905 910
Asp Gly Ser Ile Asn Thr Tyr Ser Glu Gly Ile Ala Lys Ala Thr Leu
915 920 925
Cys Asp Lys Ser Val Leu Lys Tyr Ser Tyr Lys Val Val Leu Ile Lys
930 935 940
Val Lys Gly His Val Phe Gly His Asp Glu Met Leu Thr Lys Leu Leu
945 950 955 960
Asn Val
<210> 48
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV19 CAE53038的VP5
<400> 48
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Val Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Arg Ala Ala Glu Arg Phe Ala Glu Ser Glu Val Gly Ala Ala Thr Ile
35 40 45
Asp Gly Phe Val Gln Gly Ser Val His Ser Ile Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ser
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Thr Lys Leu Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Leu Asn Lys Lys Ile Val Asp Lys Phe Gly Thr Glu Leu Glu
115 120 125
Glu Val Tyr Asp Phe Met Asn Gly Ala Ala Lys Asp Asp Glu Asn Glu
130 135 140
Ile Lys Gln Tyr Asp Ile Leu Cys Lys Ala Val Asp Ser Tyr Gly Lys
145 150 155 160
Ile Leu Arg Glu Glu Asp Lys Lys Met Leu Glu Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ser Glu Ser Arg Ser Asp Glu Glu Thr Lys Met Val Arg
180 185 190
Glu Tyr Arg Ser Lys Ile Asp Ala Leu Arg Asp Ala Val Asp Val Glu
195 200 205
Arg Glu Ser Met Thr Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ser Asp Val Leu Glu Ala Ala Ala Glu Glu Val Pro Leu Val Gly Ser
225 230 235 240
Gly Met Ala Thr Ala Ile Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Thr His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Ser Leu Ile Ala Thr Thr Leu Glu
275 280 285
Tyr Arg Asp Arg Pro Ile Pro Asp Asp Ser Leu Ala Val Ser Val Leu
290 295 300
Glu Lys Arg Arg Ala Ile Glu Glu Asn Lys Asn Glu Ile Glu His Ile
305 310 315 320
Lys Asn Glu Ile Leu Pro Arg Phe Lys Lys Ala Met Glu Glu Glu Lys
325 330 335
Glu Ile Ser Gly Ile Glu Glu Ser Val Ile His Pro Lys Val Met Met
340 345 350
Arg Phe Lys Ile Pro Arg Ser Gln Gln Pro Gln Ile His Ile Tyr Cys
355 360 365
Ser Pro Trp Asp Ser Asp Thr Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Met Asn Glu Ser Phe Phe Ile Gly Phe Asp Leu Glu Leu Asp
385 390 395 400
Leu Val His Phe Glu Asp Leu Thr Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Thr Val Val Gly Arg Thr Phe Gln Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Gly Arg Ala Met Met Asp Pro Ser Ser Gly Ala Met His Lys Arg
435 440 445
Arg Met Ser Arg Ser Arg Ser Val His Pro Ile Tyr Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Ser Tyr Ala Thr Leu Lys Ser Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Val Leu Arg Gly Pro Ile His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys His Gly Ile Lys Ile
500 505 510
Leu Asn Asp Lys Ile Asp Leu Thr Ser Leu Leu Arg Asn Ala
515 520 525
<210> 49
<211> 956
<212> PRT
<213> 人工序列
<220>
<223> BTV20 CAE51107的VP2
<400> 49
Met Glu Glu Phe Val Ile Pro Val Phe Ser Glu Gln Asp Met Pro Tyr
1 5 10 15
Ala Leu Ile Asn Gln Tyr Pro Leu Ala Ile Arg Thr Asp Val Lys Ile
20 25 30
Pro Asp Val Glu Gly Ile His Asp Leu Ala Lys Ile Pro Glu Ser Asp
35 40 45
Met Ile Asp Val Pro Lys Leu Asp Ile Val Ser Ala Leu Asn Tyr Lys
50 55 60
Pro Val Arg Asn Asp Gly Ile Val Val Pro Arg Leu Leu Asp Ile Thr
65 70 75 80
Leu Lys Ala Tyr Asp Asp Arg Lys Ser Thr Lys Asp Ala Gln Gly Ile
85 90 95
Lys Phe Met Thr Asn Ala Gly Trp Met Lys Trp Ala Ile Asp Asp Arg
100 105 110
Met Asp Ile Gln Pro Leu Lys Val Lys Leu Asp Gln His Tyr Ser Ile
115 120 125
Asp Tyr Gln Leu Phe Asn Cys Val Val Lys Ala Lys Thr Ala Asn Ala
130 135 140
Asp Thr Met Tyr Tyr Asp Tyr Phe Pro Ile Glu Asn Lys Thr Lys Lys
145 150 155 160
Cys Asn His Thr Asn Leu Asp Leu Leu Arg Ser Leu Thr Ala Val Glu
165 170 175
Met Phe His Val Leu Gln Gly Ala Ala Tyr Asn Leu Lys Ala Asn Tyr
180 185 190
Glu Leu Ile Ala Asn Ser Glu Arg Glu Ser Leu Glu Glu Ser Tyr Pro
195 200 205
Ile Gly Ser Gln Lys Trp Val His Leu Thr Arg Gly Thr Lys Ile Gly
210 215 220
Asn Ser Gly Val Pro Tyr Glu Arg Phe Ile Ser Ser Met Val Gln Val
225 230 235 240
Val Val Lys Gly Lys Val Pro Val Glu Ile Val Ser Glu Val Val Arg
245 250 255
Leu Asn Glu Ile Arg Thr Glu Trp Ile Asn Ala Gly Phe Asp Arg Ser
260 265 270
Arg Ile Arg Ala Leu Glu Leu Cys Asn Ile Leu Ser Thr Ile Gly Arg
275 280 285
Lys Met Met Asp Thr Gln Glu Glu Pro Lys Asp Glu Met Asp Leu Ser
290 295 300
Thr Arg Phe Gln Phe Lys Leu Asp Asp Lys Phe Asn Thr Ala Asp Ser
305 310 315 320
Glu Tyr Ile Val Ile Ser Arg Val Gly Gly Ala Ala Thr Asn Glu Gly
325 330 335
Arg Phe Tyr Ala Leu Ile Ala Ile Ala Ala Thr Asp Thr Gln Arg Gly
340 345 350
Arg Val Trp Arg Thr Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu Ile
355 360 365
Ala Ala Glu Cys Glu Leu Gly Asp Val Tyr Tyr Ser Leu Arg His Thr
370 375 380
Tyr Gln Trp Ser Leu Arg Pro Glu Tyr Asp Gln Arg Glu Arg Ser Leu
385 390 395 400
Glu Asn Asn Lys Tyr Val Phe Ser Arg Ile Asn Leu Phe Asp Ser Asn
405 410 415
Leu Thr Val Gly Asp Lys Val Ile His Trp Arg Tyr Asp Leu Thr Lys
420 425 430
Pro Met Asp Thr Thr Phe Asp Asp Gly Tyr Met Cys Val Ser His Glu
435 440 445
Ile Asp Gly Glu Leu Leu Cys Arg Ile Asp Asp Asp Arg Tyr Lys Glu
450 455 460
Met Leu Glu Arg Met Ile Gln Gly Gly Trp Asp Gln Glu Arg Phe Lys
465 470 475 480
Leu His Ser Ile Leu Thr Glu Pro Asn Leu Leu Thr Ile Asp Phe Glu
485 490 495
Lys Asp Ala Tyr Leu Asn Ser Arg Ser Glu Leu Val Met Pro Asp Tyr
500 505 510
Phe Asp Lys Trp Ile Asn Ser Pro Met Phe Asn Ala Arg Leu Arg Leu
515 520 525
Thr His Gly Glu Ile Ala Ser Arg Lys Thr Asp Asp Pro Trp Asn Asn
530 535 540
Arg Val Val Phe Gly Tyr Ile Lys Thr Thr Thr Glu Ser Leu Asp Tyr
545 550 555 560
Met Leu Gly Arg Tyr Tyr Asp Val Arg Leu Gln Phe Tyr Gly Asp Thr
565 570 575
Leu Ser Gln Gly Gln Arg Gln Ser Ser Val Phe Ala Tyr Ile Ser Gln
580 585 590
Arg Asp Asp Phe Gln Thr Leu Thr His Tyr Val Lys Asp Asp Ala Ile
595 600 605
Cys Pro His Ala Gly Gly Ser Ile Tyr Thr Phe Arg Lys Thr Ala Leu
610 615 620
Met Leu Met Ala Asn Tyr Glu Lys Leu Ser Pro Glu Leu His Glu Gly
625 630 635 640
Leu Glu His Gln Thr Tyr Thr His Pro Ser Asp Gly Met Asp Tyr Arg
645 650 655
Lys Tyr Val Val Asp Met Lys Asp Leu Ser Gln Leu Ile Cys Phe Val
660 665 670
Phe Asp Tyr Ile Phe Glu Lys Arg Ala Gln Leu Arg Asp Pro Lys Glu
675 680 685
Ala Arg Arg Ile Leu Tyr Leu Val Gln Asn Asn Val Gly Ser Gln Arg
690 695 700
Leu Gly Val Leu Ser Ser Ala Phe Pro Asn Leu Ala Asn Arg Leu Leu
705 710 715 720
Lys Leu Arg Glu Ile His Phe Ile Tyr Asp Met Asn Val Ile Asn Phe
725 730 735
Leu Pro Leu Leu Phe Leu Val Gln Asp Asn Ile Ser Tyr Trp His Arg
740 745 750
Gln Trp Ala Ile Pro Met Val Leu Phe Asp Glu Thr Ile Lys Leu Ile
755 760 765
Pro Val Glu Val Gly Ala Tyr Ala Asn Arg Phe Gly Phe Lys Ser Phe
770 775 780
Phe Asn Phe Thr Arg Phe His Pro Gly Asp Ser Arg Lys Lys Gln Ile
785 790 795 800
Ala Asp Asp Val His Lys Glu Phe Gly Thr Ile Cys Tyr Glu Tyr Tyr
805 810 815
Met Asn Thr Arg Met Ser Gln Gly Gly Val Asn Ile Ser Val Val Thr
820 825 830
Thr Lys Leu Asp Val Leu Lys Ile His Leu Ala Ser Leu Cys Ala Gly
835 840 845
Leu Ala Asp Ser Ile Val Tyr Thr Leu Pro Val Ala His Pro Arg Lys
850 855 860
Cys Ile Val Leu Ile Val Val Gly Asp Asp Lys Leu Glu Pro Gln Ile
865 870 875 880
Arg Ala Glu Gln Val Leu Ser Lys Tyr Tyr Tyr Ser Arg Lys His Val
885 890 895
Leu Gly Val Ile Ser Ile Ser Val Ser Gln Asp Gly Lys Leu Lys Val
900 905 910
Tyr Ser Ser Gly Ile Val Arg His Arg Leu Cys Glu Lys Ser Ile Leu
915 920 925
Lys Tyr Lys Cys Lys Thr Ile Leu Ile Arg Met Pro Gly His Val Phe
930 935 940
Gly Asn Asp Glu Leu Met Thr Lys Leu Leu Asn Ile
945 950 955
<210> 50
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV20 CAE53039的VP5
<400> 50
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ala Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Ser Ala
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Met Gln
85 90 95
Ala Lys Ile Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Ile Arg
100 105 110
Leu Lys Tyr Asn Lys Glu Ile Thr Asp Ala Phe Gly Lys Glu Leu Asn
115 120 125
Glu Val Tyr Glu Phe Met Asn Gly Glu Thr Lys Glu Glu Glu Val Val
130 135 140
Gln Glu Gln Tyr Ser Met Leu Cys Lys Ala Val Asp Ser Tyr Glu Lys
145 150 155 160
Ile Leu Lys Ala Glu Asp Ser Lys Met Ala Val Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Ser Glu Arg Ser Glu Asp Glu Ile Lys Met Val Lys
180 185 190
Glu Tyr Arg His Lys Ile Asp Ala Leu Lys Asn Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Val Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Met Arg Thr Pro Lys Ile Glu Pro Thr Ile Ile Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Lys Asp Ile Pro Asp Asp Arg Leu Ala Ile Ser Val Leu
290 295 300
Asn Lys Lys Ala Ala Val Ala Asp Asn Cys Asn Glu Ile Ser His Ile
305 310 315 320
Lys Gln Glu Ile Leu Pro Lys Phe Arg Lys Ile Met Asp Glu Glu Lys
325 330 335
Glu Ile Glu Gly Ile Glu Asp Lys Val Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Ile Pro Arg Thr Gln Gln Pro Gln Ile His Ile Tyr Ala
355 360 365
Ala Pro Trp Asp Ser Asp Glu Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Arg Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Thr Ser His Trp His Ala Leu Gly Asn
405 410 415
Ala Gln Glu Ala Thr Gly Arg Thr Leu Thr Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ser Ala Thr Ser Met Phe Ser Ser Val Ile His Thr Arg
435 440 445
Arg Met Ile Arg Ser Arg Ala Val His Pro Ile Phe Leu Gly Ser Met
450 455 460
His Tyr Asp Val Thr Tyr Glu Val Leu Lys Asn Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Ile
500 505 510
Leu Gly Asp Lys Leu Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 51
<211> 955
<212> PRT
<213> 人工序列
<220>
<223> BTV21 CAE51108的VP2
<400> 51
Met Asp Glu Leu Ala Ile Pro Ile Ile Asp Gly Arg Phe Pro Lys Glu
1 5 10 15
Ala Leu Leu Glu Tyr Cys Cys Ile Val Glu Leu Asn Pro Ala Ser Glu
20 25 30
Glu Asp Asp Val Asp Val Thr Thr Ile Pro Gln Lys Asp Met Val Asp
35 40 45
Ile Pro Asp Ala Pro Ile Arg Glu Ala Leu Asn Tyr Leu Pro Lys Arg
50 55 60
Asn Asn Gly His Val Leu Thr Arg Val Val Asp Ile Ser Leu Ser Gly
65 70 75 80
Tyr Asp Arg Arg Lys Tyr His Arg Thr Ser Thr Gly Gly Asp Phe Leu
85 90 95
Ser Pro Glu Glu Trp Leu Asn Trp Met Ile Arg Asp Ser Val Asp Val
100 105 110
Gln Pro Leu Lys Ala Ser Leu Glu Phe Pro Ser Lys Ile Lys His Ser
115 120 125
Leu Phe Ser Ser Asn Val Tyr Val Ser Val Lys Lys Ala Asp Thr Ile
130 135 140
Ser Tyr His Ile Glu Ala Lys Glu Val Glu Thr Arg Ala Cys Asp His
145 150 155 160
Ala Arg Val Ala Met Trp Asn Asn Leu Val Arg His Glu Met Met His
165 170 175
Ser Ala Gln Glu Pro Ala Tyr Ile Leu Lys Pro Thr Leu Asp Ile Ile
180 185 190
Val His Ser Glu Arg Ala Ser Thr Asp Val Pro Phe Glu Leu Val Gly
195 200 205
Gln Arg Tyr Ile Ser Ile Gly Lys Asn His Arg Ile Val Leu Gly Asp
210 215 220
Asp Ala Tyr Arg Lys Thr Leu Asp Gly Leu Thr Arg Leu Arg Val Gln
225 230 235 240
Gly Arg Ile Pro Glu Leu Ile Arg Asn Glu Ile Gln Gln Leu His Gln
245 250 255
Ile Arg Asp Ala Trp Ile Thr Asp Ser Tyr Asn Pro Arg His Ile Arg
260 265 270
Ser Leu Glu Leu Cys Arg Ile Leu Ser His Ile Gly Arg Lys Met Val
275 280 285
His Ile Glu Glu Glu Pro Lys Asp Glu Ser Asp Leu Ser Val Lys Phe
290 295 300
Gln Phe Lys Leu Asp Glu Lys Phe Arg Ile Asn Asp Asp Glu Arg Asn
305 310 315 320
Val Ile Phe Thr Ser Lys Gly Gln Arg Asn Asp Gln Gln Arg Phe Phe
325 330 335
Val Leu Ile Met Ile Ala Ala Ser Asp Thr Tyr Asn Ser Arg Ile Trp
340 345 350
Trp Thr Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu Ile Ala Ser Glu
355 360 365
Thr Ser Leu Gly Asp Val Tyr Trp Thr Leu Arg His Phe Tyr Asp Trp
370 375 380
Ser Val Arg Pro Val Tyr Ser Pro Arg Gln Lys Glu Arg Glu Asp Asp
385 390 395 400
Lys Tyr Leu Tyr Gly Arg Val Asn Leu Phe Asp Leu Asp Ala Asp Pro
405 410 415
Gly Thr Gln Ile Ile His Trp Glu Tyr Lys Pro Ile Thr Ser Thr Val
420 425 430
Lys Thr Thr Tyr Glu Arg Gly Asn Met Cys Asp Leu Phe Pro Asp Val
435 440 445
Asp Gly Ile Thr Thr Lys Phe Asn Lys Thr Ala Tyr Ile Asp Met Ile
450 455 460
Ser Glu Leu Leu Asn Gly Gly Trp Asn Thr Arg Glu Phe Lys Met Tyr
465 470 475 480
Lys Ile Leu Glu Ser Val Gly Asn Val Leu Thr Ile Asp Phe Glu Lys
485 490 495
Asp Thr Lys Leu Asn Ser Arg Ser Glu Leu Ser Leu Pro Tyr Tyr Tyr
500 505 510
Asp Lys Trp Ile Tyr Ala Pro Met Phe Asn Ala Arg Leu Lys Ile Thr
515 520 525
Gln Thr Glu Ile Ala Gln Arg Lys Ala Asp Asp Pro Ser Ile Tyr Arg
530 535 540
Thr Leu Ala Pro Ile Ser Ser Asp Pro Ile Asp Leu Gln Arg Met Cys
545 550 555 560
Leu Ala Asn Tyr Tyr Asp Val Arg Pro Ala Met His Gly Arg Ala Leu
565 570 575
Ser Lys Arg Gln Glu Gln Ser Ala Tyr His Lys Asn Leu Ala Asn Leu
580 585 590
Ala Asp Tyr Gln Asp Ile Leu Lys Arg Arg Gly Tyr Arg Lys Val Glu
595 600 605
Asn Pro Cys Pro Leu Ile Thr Cys Ser Tyr Ile Leu Glu Lys Ser Ala
610 615 620
Leu Leu Trp Ile Asp Ile Met Glu Lys His Val Gly Tyr Ala Pro Glu
625 630 635 640
Ser Glu Ile Glu Phe Asp Phe Pro Lys Val Asn Thr Ser Thr Arg Ile
645 650 655
Gly Thr Glu Arg Ile Val Asp Ile Ser Gln Leu Val Val Leu Met Ile
660 665 670
Asp Trp Leu Tyr Glu Lys Arg Gln Thr Thr Arg Ser Val Asp Glu Ala
675 680 685
Arg Trp Thr Val Gln Val Ile Arg Arg Val Thr Gly Lys Lys Arg Leu
690 695 700
Glu Ala Ile Ser Arg Glu Phe Pro Asn Phe Gly Glu Ala Val Ser Arg
705 710 715 720
Leu Ala Cys Pro Lys Thr Val Arg Asp Val Met Val Ile Asn Phe Leu
725 730 735
Pro Phe Leu Phe Leu Leu Gly Asp Asn Met Ala Tyr Glu His Arg Gln
740 745 750
Trp Ser Val Pro Ile Ile Leu Tyr Ala Asp Thr Leu Trp Phe Leu Pro
755 760 765
Val Glu Val Gly Ala Tyr Tyr Asn Arg Phe Gly Ile Val Ser Phe Leu
770 775 780
Glu Tyr Leu Thr Phe Phe Pro Ser Asn His Met Arg Thr Val Ala Leu
785 790 795 800
Asp Glu Ala Glu Ser Lys Val Gly Lys Leu Ile Leu Glu Phe Tyr Leu
805 810 815
Asn Thr Thr Ile Tyr Leu Gly Asn Ile Gln Gln Ser Val Ile Ser Thr
820 825 830
Lys Gln Ile Leu Tyr Glu Thr Tyr Leu Ser Ser Leu Cys Gly Gly Phe
835 840 845
Thr Glu Gly Leu Val Trp Tyr Leu Pro Ile Thr His Pro Gln Lys Cys
850 855 860
Val Val Ala Ile Glu Ile Cys Asp Asp Arg Val Ser Ala Gly Tyr Arg
865 870 875 880
Cys Asp Lys Leu Arg Ser Arg Phe Pro Leu Ser Leu Asp His Leu Arg
885 890 895
Gly Ile Val Ile Leu Ser Val Ser Lys Ser Arg Ala Ile Ser Ala Tyr
900 905 910
Ser Glu Gly Ile Val Ser His Arg Leu Cys Lys Lys Asn Val Leu Gly
915 920 925
Phe Thr Cys Arg Val Leu Leu Leu Lys Phe Ser Gly His Val Phe Gly
930 935 940
Asn Asp Glu Met Leu Thr Lys Leu Leu Asn Val
945 950 955
<210> 52
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV21 CAE53040的Vp5
<400> 52
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Ile Val Gln Gly Ser Val His Ser Ile Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ala
65 70 75 80
Gly Asp Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Met Lys Ile Lys Glu Ile Glu Glu Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Gly Lys Glu Ile Thr Lys Lys Phe Gly Glu Glu Leu Glu
115 120 125
Glu Ile Tyr Gln Phe Met Asn Gly Glu Val Lys Asp Glu Glu Glu Gln
130 135 140
Glu Glu Gln Tyr Lys Val Leu Cys Lys Ala Val Asn Ser Tyr Glu Lys
145 150 155 160
Leu Leu Ile Ala Glu Asn Asp Lys Met Arg Ile Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Ala Glu Arg Thr Glu Ala Glu Ser Thr Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Ala Ala Ile Glu Ile Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Val Gly Ser
225 230 235 240
Gly Met Ala Thr Ala Ile Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Thr Met Val Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Glu Asp Ile Pro Asp Lys Lys Leu Ala Met Ser Ile Leu
290 295 300
Ala Lys Asn Asn Ala Ile Thr Ala Asn Thr Arg Glu Ile Gln His Ile
305 310 315 320
Lys Glu Glu Ile Leu Pro Lys Phe Lys Lys Val Met Asp Glu Glu Lys
325 330 335
Glu Leu Glu Gly Ile Asp Asp Lys Lys Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Val Pro Arg Ser Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
Phe His Ala Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Ala Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Glu Ala Lys Gly Arg Thr Leu Asn Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ala Ile Gly Ser Thr Tyr Thr Ser Pro Met His Ala Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val Tyr Pro Ile Tyr Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Asp Thr Leu Lys Ser Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Val
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Lys Asn Ala
515 520 525
<210> 53
<211> 951
<212> PRT
<213> 人工序列
<220>
<223> BTV22 CAE51109的VP2
<400> 53
Met Glu Asp Phe Pro Ile Cys Ala Ile Glu Arg Glu Ser Val Tyr Asp
1 5 10 15
Glu Asp Phe Ile Met Arg Tyr Pro Val Ile Val Asp Leu Lys Glu Cys
20 25 30
Val Ala Asp Arg Arg Asn Lys Thr Asp Val Ser Lys Leu Glu Glu Ile
35 40 45
Lys Gly Val Cys Val Arg Ser Glu Gly Val Arg Val Ala Leu Thr Ser
50 55 60
Arg Pro Asp Pro Leu Ser His Val Ile Thr Pro Ser Ala Leu Asn Val
65 70 75 80
Leu Ile Gln Ala Tyr Asp Arg Lys His Lys Val Ser Leu Arg Lys Arg
85 90 95
Phe Glu Asp Gly Glu Gly Val Thr Glu Tyr Asp Leu Asp Thr Trp Val
100 105 110
Lys Lys Arg Leu Glu Glu Gln Ala Asp Leu Gln Ala Val Tyr His Thr
115 120 125
Ile Asp Ala Asn Arg Gly Lys Leu Lys Phe Lys Thr Leu Met Gly Thr
130 135 140
Ile Arg Leu Asp Ser Asn Tyr Ala Glu Thr Val Gly Tyr Asp Tyr Ala
145 150 155 160
Pro Val Ser Lys Ala Gly Cys Asn His Gly Asn Glu Asp Pro Leu Tyr
165 170 175
Asp Phe Ile Leu Ser Gly Ala Phe Gln Val Ser Lys Thr Ala Gly Tyr
180 185 190
Thr Leu Lys Gln Thr Tyr Arg Leu Thr Val Arg Thr Glu Val Arg Glu
195 200 205
Asn Asn Lys Asn Glu Leu Val Val Asn Glu Ile Tyr Thr Pro Gly Ile
210 215 220
Val Arg Gly Glu Gln Ile Arg Asp Met Thr Ser Val Ala Tyr Met Arg
225 230 235 240
Phe Arg Asp Gly Trp Ile Arg Ala Ile Ile Gln Pro Gln Ile Val Glu
245 250 255
Glu Leu Arg Ala Ser Lys Asp Ala Leu Asp Arg Ile Ser Asn Glu Trp
260 265 270
Tyr Ser Ser Arg Asn Thr Pro Asp Val Val Glu Ile Cys Arg Ile Val
275 280 285
Ser Ser Ile Gly Arg Arg Met Trp Asn Ser Glu Glu Thr Pro Val Asp
290 295 300
Asp Ala Met Arg Ser Arg Val Phe Gln Glu Lys Leu Arg Leu Leu Phe
305 310 315 320
Arg Val Gly Asn Ser Glu Tyr Asp Thr Ile Gln Ser Ile Ser Ser Gly
325 330 335
Gln Thr Asp Leu Lys Lys Phe Tyr Ala Leu Leu Ala Ile Ala Ala Thr
340 345 350
Asp Ser Tyr Arg Trp Arg Ile Trp Trp Ser Asn Pro Tyr Pro Cys Leu
355 360 365
Arg Gly Thr Ile Ile Ala Cys Glu Met Glu Leu Gly Asp Val Tyr Lys
370 375 380
Thr Leu Arg Ser Thr Phe Arg Trp Ser Leu Arg Pro Glu Tyr Ala Asn
385 390 395 400
Arg Ser Glu Ile Asp Arg Glu Lys Lys Ala Tyr Pro Tyr Gln Arg Ile
405 410 415
Asn Leu Phe Glu Ser Lys Leu Thr Pro Gly Thr Arg Ile Ile Asn Trp
420 425 430
Leu Val Thr Arg Val Pro Leu Glu Lys Pro Asp Ile Ser Thr Gly Arg
435 440 445
Val Cys Met Thr Ser Thr Glu Ser Asn Tyr Ile Leu Lys Ile Asp Asp
450 455 460
Ala Arg Tyr Lys Gln Met Ile Ser Gln Ile Ile Glu Arg Gly Trp Glu
465 470 475 480
Lys Glu Gln Leu Lys Phe Glu Lys Ile Val Gln Asp Asp Gly Asn Val
485 490 495
Phe Lys Met Glu Phe Glu Lys Asp Ala Cys Met Asp Glu Lys Ser His
500 505 510
Leu Val Met Pro Tyr Tyr Tyr Asp Lys Glu Ile His Cys Pro Ile Tyr
515 520 525
His Gln Lys Ile Lys Ile Thr Glu Ala Gln Met Ala Asn Glu Trp Ser
530 535 540
Asp Asp Pro Trp Val Asn Arg Val Val Ser Gly Tyr Leu Gly Asp His
545 550 555 560
Val Glu Thr Tyr Gly Leu Gly Tyr Asn His Ile Phe Asp Thr Arg Val
565 570 575
Pro Leu Lys Gly Asn Leu Leu Glu Gln Glu Gln Glu Ile Ser Ser Tyr
580 585 590
His Glu His Leu Arg Ser Asp Glu Ser Lys Glu Asp Asp Thr His Cys
595 600 605
Ala Leu Arg Ile Phe Ser Asp Lys Ala Ile Arg Met Leu Phe Ile Ala
610 615 620
Ile Tyr Lys Lys Leu Ile His His Ile Pro Lys Glu Glu Tyr Ala Thr
625 630 635 640
Arg Glu Glu Glu Leu Ile Gln Tyr Ile Ser Met Val Glu Arg Val Arg
645 650 655
Ser Trp Asp Gln Phe Ile Val Phe Ile Phe Asp Phe Phe Phe Glu Asn
660 665 670
Arg Lys Gly Ile Arg Thr Met Glu Glu Ala Thr Gln Leu Ala Lys Ser
675 680 685
Ile Arg Gly Ser Val Gly Ala Met Arg Trp Thr Ile Phe Ser Ser Ser
690 695 700
Phe Pro Thr Ser Tyr Arg Leu Met Lys Gln Ile Leu Val Ala Lys Arg
705 710 715 720
Trp Gly Asp Cys Tyr Ile Met Asn Phe Leu Pro Leu Leu Ile Cys Gly
725 730 735
Gly Thr Ser Ile Ser Tyr Leu His Arg Glu Trp Ser Tyr Pro Met Ile
740 745 750
Val Thr Phe Glu Asp Gly Ile Lys Ile Val Pro Thr Met Val Gly Arg
755 760 765
Ser Leu Pro Gln Gly Ser Leu Gly Glu Trp Val Ser Phe Ile Ser Phe
770 775 780
Phe Met Gly Thr Val His Glu Lys Ile Lys Val Glu Glu Asp Glu Leu
785 790 795 800
Glu Val Leu Arg Arg Cys Val Glu Phe Tyr Arg Asp Val Lys Met Val
805 810 815
Asp Val Ile Lys Asp Thr Pro Val Arg Met Thr Lys Tyr Ile Thr Cys
820 825 830
Asp Leu Ala Phe Gly Ser Gly Cys Gly Gly Ile Ser Glu Ser Ile Ser
835 840 845
Phe Ile Leu Pro Val Thr His Pro Asn Arg Ser Leu Val Val Phe Val
850 855 860
Ile Ser Asp Asp Lys Leu Ser Pro Ser Gln His Ala Ala Arg Ala Lys
865 870 875 880
Arg Arg Phe Lys Gly Thr Cys Gln Tyr Ile Asp Asp Val Ile Val Leu
885 890 895
Gln Met Lys Arg Pro Gly Leu Val Gly Ser Val Trp Ser Glu Lys His
900 905 910
Ser Lys Ile Lys Val Cys Arg Arg Asn Phe Leu Asn Tyr Asp His Lys
915 920 925
Val Ile Leu Thr Lys Phe Ala Gly Leu Val Tyr Gly Asn Tyr Glu Leu
930 935 940
Leu Thr Lys Leu Ala Asn Leu
945 950
<210> 54
<211> 529
<212> PRT
<213> 人工序列
<220>
<223> BTV22 CAE53041的VP5
<400> 54
Met Met Gly Lys Ile Ile Lys Ser Leu Gly Lys Phe Gly Lys Lys Val
1 5 10 15
Gly Asn Ala Leu Thr Ser Lys Thr Ala Gln Lys Ile Tyr Lys Thr Ile
20 25 30
Gly Asn Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala
35 40 45
Ile Asp Gly Leu Ile Gln Gly Ser Val His Ser Ile Met Thr Gly Glu
50 55 60
Ser Tyr Gly Glu Ser Val Lys Gln Ala Val Ile Leu Asn Val Leu Gly
65 70 75 80
Gly Gly Asp Thr Ile Ala Asp Pro Leu Ser Pro Gly Glu Asn Glu Ile
85 90 95
Tyr Arg Lys Leu Arg Gln Leu Glu Glu Glu Gln Lys Gly Glu Val Val
100 105 110
Arg Thr Lys His Asn Thr Glu Ile Leu Asp Lys Phe Gly Arg Asp Leu
115 120 125
Asp Glu Val Tyr Lys Phe Ala Thr Gln Glu His Lys Val Gly Glu Asp
130 135 140
Glu Glu Asp Gln Ile Lys Met Leu Glu Lys Ala Leu Lys Ser Tyr Val
145 150 155 160
Gly Val Phe Lys Ala Glu Asn Glu Ser Ile Asn Lys Leu Lys Gln Ala
165 170 175
Leu Tyr Leu Glu Ser Thr Glu Arg Thr Asp Glu Glu Thr Lys Met Ile
180 185 190
Ser Glu Tyr Arg Asp Lys Ile Asp Ala Leu Arg Gly Ala Ile Glu Val
195 200 205
Glu Lys Glu Gly Leu Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met
210 215 220
Ser Ala Asp Val Leu Glu Ala Ala Ala Glu Glu Val Pro Leu Phe Gly
225 230 235 240
Ala Gly Val Ala Thr Ser Ile Ala Thr Ala Arg Ala Ile Glu Gly Ala
245 250 255
Tyr Lys Ile Lys Ala Val Ile Ser Ala Leu Ser Gly Ile Asp Leu Thr
260 265 270
His Leu Ser Val Pro Lys Val Gln Pro Lys Thr Leu Glu Ala Ile Leu
275 280 285
Asp Thr Pro Lys Gly Lys Asp Ile Ser Asp Leu Arg Leu Thr Glu Gly
290 295 300
Leu Ile Ala Lys Met Asp Lys Ile Ser Glu Asn Ile Ser Glu Val Asp
305 310 315 320
His Ile Gln Asn Val Ile Val Pro Lys Leu Lys Lys Ala Ala Glu Glu
325 330 335
Asp Lys Glu Asn Ile Thr Asn Trp Ser Glu Lys Arg Ile His Pro Lys
340 345 350
Thr Val Gln Arg Phe Lys Ile Pro Gln Ala Gln Arg Pro Ser Met His
355 360 365
Ile Tyr Ala Ala Pro Trp Asp Ser Asp Glu Val Phe Ile Phe His Ile
370 375 380
Ile Ser Pro His His Lys Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu
385 390 395 400
Glu Ile Glu Tyr Val Phe Tyr Glu Asp Leu Thr Gln His Trp His Ala
405 410 415
Leu Gly Ala Ala Gln Ser Ala Tyr Gly Arg Thr Phe Arg Glu Ala Tyr
420 425 430
Arg Glu Phe Phe Met Leu Ala Ser Asn Val Ala Gly Gln Gly Ala Ile
435 440 445
His Gln Lys Arg Leu Leu Arg Ser Arg Ser Ser His Pro Ile Tyr Leu
450 455 460
Gly Met His Asp Tyr Glu Val Ser Tyr Glu Gln Ile Lys Met Asn Ala
465 470 475 480
Met Ala Leu Val Ser Asn Glu Asp Leu Gln Met His Val Leu Arg Gly
485 490 495
Pro Val His Phe Gln Arg Arg Thr Ile Val Ala Ala Leu Lys Tyr Gly
500 505 510
Ile Lys Ile Leu Thr Gln Pro Asp Asp Val Ser Val Met Leu Arg Asn
515 520 525
Ala
<210> 55
<211> 957
<212> PRT
<213> 人工序列
<220>
<223> BTV23 CAE51110的VP2
<400> 55
Met Asp Glu Leu Ala Ile Pro Ile Tyr Thr Asn Val Phe Pro Ala Glu
1 5 10 15
Leu Leu Asp Gly Tyr Asp Tyr Ile Ile Asp Val Ser Thr Arg Ile Asp
20 25 30
Glu Pro Ala Gly Arg His Asp Val Thr Gln Ile Pro Asn Ser Ala Met
35 40 45
Phe Asp Val Gln Asn Glu His Ile Arg Asp Ala Ile Ile Ser Lys Pro
50 55 60
Val Ser Asn Asp Gly Tyr Val Leu Pro Arg Ile Leu Asp Ile Ser Leu
65 70 75 80
Arg Ala Phe Asp Asp Arg Lys Arg Ile Ile Thr Ser Gly Gly Gln Ser
85 90 95
Glu Tyr His Thr Lys Ala Asp Trp Leu Gln Trp Met Ile Asp Asp Ala
100 105 110
Met Asp Val Gln Pro Leu Lys Val Asp Thr Ala His Thr Arg Thr Arg
115 120 125
Val Ser His Ala Leu Phe Asn Cys Thr Val Arg Leu His Ala Lys Lys
130 135 140
Ala Asp Thr Ala Ser Tyr His Val Glu Ser Ile Glu Ile Glu Pro Asn
145 150 155 160
Gly Cys Asn His Ala Trp Ile Gln Arg Val His His Leu Val Asn Ile
165 170 175
Glu Met Leu His Cys Ser Gln Glu Val Ala Tyr Met Leu Lys Pro Thr
180 185 190
Tyr Glu Thr Ile Val His Ala Glu Arg Ala Ser Thr Ser Asp Thr Phe
195 200 205
Asn Gly Arg Thr Ile Glu Leu Ser Arg Gly His Arg Val Gln Leu Gly
210 215 220
Asp Ala Ala Tyr Glu Lys Leu Lys Asn Gly Leu Val Gln Thr Arg Ile
225 230 235 240
Glu Gly Lys Lys Pro Gln Leu Val Glu Gly Glu Ile Asn Ser Leu Asn
245 250 255
Asp Ile Arg Gln Arg Trp Ile Asn Ser Gly His Asp Arg Arg Glu Ile
260 265 270
Lys Ala Leu Glu Leu Cys Lys Ile Leu Ser Thr Ile Gly Arg Lys Met
275 280 285
Leu Asn Thr Glu Glu Glu Pro Lys Asn Glu Ala Asp Leu Ser Val Lys
290 295 300
Phe Gln Met Glu Leu Asp Glu Ile Phe Arg Pro Gly Asn Asn Glu Arg
305 310 315 320
Thr Asn Ile Phe Gly Gly Gly Asn His Arg Lys Asp Glu Asp Arg Phe
325 330 335
Tyr Val Leu Ile Met Ile Ala Ala Ser Asp Thr Tyr Lys Gly Arg Ile
340 345 350
Trp Trp Ser Asn Pro Tyr Pro Cys Leu Arg Gly Ser Leu Ile Ala Ala
355 360 365
Glu Ala Gln Leu Gly Asp Val Tyr Asn Thr Met Arg Asn Trp Tyr Gln
370 375 380
Trp Ser Val Arg Pro Thr Tyr Thr Pro Tyr Asp Arg Ser Arg Glu Ser
385 390 395 400
Asp Lys Tyr Val Tyr Ser Arg Val Asn Leu Phe Asp Ser Thr Leu Arg
405 410 415
Pro Gly Asp Lys Ile Ile His Trp Glu Tyr Lys Leu Leu Asn Glu Val
420 425 430
Arg Glu Val Cys Ile Lys Asp Gly Asn Glu Cys Asp Leu Phe Pro Glu
435 440 445
Asp Glu Glu Met Thr Thr Lys Phe Asn Glu Glu Lys Tyr Thr Glu Met
450 455 460
Lys Gly Gln Ile Ile Gln Asp Gly Trp Asn His Arg Asp Phe Lys Met
465 470 475 480
His Lys Ile Leu Gln Asp Gly Ala Asn Val Leu Thr Ile Asp Phe Glu
485 490 495
Lys Asp Ala Arg Ile Gly Asn Gly Ser Val Leu Thr Leu Pro Glu Tyr
500 505 510
Tyr Asn Lys Trp Ile Val Ala Pro Met Phe Asn Ala Lys Leu Arg Ile
515 520 525
Ser Glu Thr Val Ile Ala Lys Ala His Ser Asp Asp Pro Ala Val Ala
530 535 540
Arg Thr Ala Lys Ser Phe Asp His Asp Pro Phe Asn Leu Gln Arg Tyr
545 550 555 560
Cys Leu Ala Arg Tyr Tyr Asp Val Arg Pro Gly Leu Met Gly Arg Ala
565 570 575
Leu Ser Lys Glu Gln Glu Gly Ser Ala Ile Ala Asn Lys Val Ala Ala
580 585 590
Leu Glu Asp Tyr Arg Asp Val Ile Ser Arg Arg Leu Gly Tyr Val Glu
595 600 605
Arg Glu Lys Arg Cys Leu Thr Glu Thr Ala Gln Tyr Ile Leu Glu Lys
610 615 620
Thr Ser Leu Tyr Leu Leu Asn Val Leu Ser Met His Val Lys Pro Thr
625 630 635 640
Ile Asp Met Asp Ile Lys Phe Lys His Pro Ser Ile Asp Thr His Leu
645 650 655
Asp Ile Asp Thr Trp Lys Ile Ala Asp Ile Ser Gln Leu Ile Val Leu
660 665 670
Leu Phe Asp Tyr Leu Phe Glu Ser Arg Lys Glu Ile Arg Ser Val Thr
675 680 685
Glu Ala Arg Trp Ile Leu Phe Lys Val Arg Ser Val Ser Gly Gln Glu
690 695 700
Arg Leu Asn Met Ile Gln Ser Phe Phe Pro Arg Phe Gly Arg Val Val
705 710 715 720
Arg Asp Ile Ala Lys Ala Lys Ile Thr Gln Asp Val Ala His Leu Asn
725 730 735
Phe Phe Pro Leu Phe Phe Ile Ile Gly Asp Asn Met Ala Tyr Ala His
740 745 750
Arg Gln Trp Ser Ile Pro Val Leu Leu Tyr Ala His Asp Ile Arg Ile
755 760 765
Ile Pro Leu Glu Ile Gly Ala Tyr Asn Asn Arg Phe Gly Phe Thr Ser
770 775 780
Tyr Leu Glu Tyr Met Thr Phe Phe Pro Ser His Ala Thr Arg Glu Ala
785 790 795 800
Val Leu Asp Glu Ser Ile Lys Glu Cys Ser Leu Ala Leu Thr Asn Phe
805 810 815
Tyr Leu Asn Thr Asp Ile His Ser Gly Glu Ile Met Arg Asn Val Val
820 825 830
Thr Thr Lys Arg Leu Leu Tyr Glu Thr His Leu Ala Ser Leu Cys Gly
835 840 845
Gly Tyr Ser Asp Gly Leu Leu Trp Tyr Val Pro Ile Thr His Pro Ser
850 855 860
Lys Cys Leu Val Ala Phe Glu Val Ala Asp Asp Val Val Pro Tyr Ser
865 870 875 880
Val Arg Arg Glu Arg Ile Ile Ser Arg Phe Pro Leu Ser Ser Lys His
885 890 895
Leu Arg Gly Ile Ala Leu Ile Ala Val Asn Arg Asn Gln Lys Val Ser
900 905 910
Val Gln Thr Glu Gly Ile Val Thr His Arg Leu Cys Lys Lys Asn Leu
915 920 925
Leu Lys Tyr Val Cys Asp Val Val Leu Phe Lys Phe Ser Gly Tyr Val
930 935 940
Phe Gly Asn Asp Glu Met Leu Thr Lys Leu Leu Asn Val
945 950 955
<210> 56
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV23 CAE53043的VP5
<400> 56
Met Gly Lys Val Ile Arg Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ser
65 70 75 80
Gly Glu Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Ala Lys Leu Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Asp Glu Ile Lys Lys Lys Phe Gly Glu Glu Leu Glu
115 120 125
Gln Val Tyr Asp Phe Met Ser Gly Ala Ala Lys Glu Glu Ala Gly Asp
130 135 140
Glu Lys Gln Phe Asp Ile Leu Asn Lys Ala Val Asn Ser Tyr Asn Lys
145 150 155 160
Ile Leu Ala Glu Glu Asp Leu Gln Met Arg Arg Leu Ala Thr Ala Leu
165 170 175
Gln Lys Glu Val Gly Glu Arg Thr Arg Val Glu Thr Thr Met Val Lys
180 185 190
Glu Tyr Arg Asp Lys Ile Asp Ala Leu Arg Ser Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Thr His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Thr Val Val Ser Thr Ile Leu Glu
275 280 285
His Arg Thr Arg Asp Ile Pro Asp Asn Ala Leu Ala Val Ser Val Leu
290 295 300
Ser Lys Asn Arg Val Ile Gln Glu Asn His Lys Glu Leu Met His Ile
305 310 315 320
Lys Glu Glu Ile Leu Pro Arg Phe Lys Lys Ala Met Asp Glu Glu Lys
325 330 335
Glu Ile Cys Gly Ile Glu Asp Lys Val Ile His Pro Lys Val Met Met
340 345 350
Lys Phe Lys Ile Pro Arg Ala Gln Gln Pro His Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Met Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Ser Ile Asp
385 390 395 400
Leu Val His Tyr Glu Asp Leu Thr Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Met Ala Val Gly Arg Thr Leu Thr Glu Ala Tyr Lys Glu Phe
420 425 430
Leu Asn Leu Ala Ile Ser Asn Ala Tyr Gly Thr Gln Met His Thr Arg
435 440 445
Arg Leu Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Leu
450 455 460
His Tyr Asp Val Ser Phe Ala Asp Leu Arg Gly Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Cys Lys Ile
500 505 510
Leu Gly Asp Arg Leu Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 57
<211> 955
<212> PRT
<213> 人工序列
<220>
<223> BTV24 CAE51111的VP2
<400> 57
Met Glu Glu Phe Val Ile Pro Val Tyr Ser Glu Asn Glu Ile Pro Tyr
1 5 10 15
Thr Leu Leu Asn Arg Tyr Leu Leu Ala Ile Arg Thr Asp Val Lys Ile
20 25 30
Asn Asp Val Glu Lys Lys His Asn Val Ile Lys Ile Pro Asp Ser Asp
35 40 45
Met Ile Asp Ile Pro Lys Ile Ser Ile Val Asp Ala Leu Ser Tyr Arg
50 55 60
Pro Thr Arg Asn Asp Gly Ile Val Val Pro Arg Leu Leu Asp Ile Thr
65 70 75 80
Leu Arg Ala Tyr Asp Asp Arg Lys Ala Thr Lys Asn Ala Lys Gly Val
85 90 95
Glu Phe Met Thr Asn Ala Lys Trp Met Lys Trp Ala Ile Asp Asp Arg
100 105 110
Met Asp Ile Gln Pro Leu Lys Val Thr Leu Asp Asp His Tyr Ser Val
115 120 125
Asn His Gln Leu Phe Asn Cys Val Val Lys Ala Lys Pro Ala Asn Ala
130 135 140
Asp Thr Val Tyr Tyr Asp Tyr Tyr Pro Leu Glu Ser Lys Met Lys Lys
145 150 155 160
Cys Asn His Thr Asn Leu Asp Met Leu Arg Ser Leu Thr Thr Met Glu
165 170 175
Met Tyr His Ile Leu Gln Gly Ala Ala Tyr Gly Leu Lys Ser Thr Tyr
180 185 190
Glu Leu Lys Ala His Ala Glu Arg Glu Asn Thr Gly Glu Ser Tyr Pro
195 200 205
Ile Gly Glu Arg Leu Leu Gln Ile Pro Arg Gly Thr Lys Ile Gly Ser
210 215 220
Arg Gly Glu Pro Tyr Asn Lys Phe Val Ala Gly Leu Val Lys Val Thr
225 230 235 240
Ile Lys Gly Lys Val Pro Ala Glu Ile Gly Glu Glu Met Asn Gln Leu
245 250 255
Asn Arg Ile Lys Glu Glu Trp Lys Ser Ala Ser His Asp Arg Ser Arg
260 265 270
Ile Arg Ala Leu Glu Leu Cys Lys Ile Leu Ser Thr Ile Gly Arg Lys
275 280 285
Met Leu Asp Ala Lys Glu Glu Pro Lys Asp Glu Met Asp Leu Ser Thr
290 295 300
Arg Phe Gln Phe Lys Leu Asp Glu Lys Phe Thr Lys Thr Asp Ser Glu
305 310 315 320
His Ile Asn Ile Phe Arg Ala Gly Gly Pro Ala Thr Asp Asp Gly Arg
325 330 335
Phe Tyr Ala Leu Ile Ala Ile Ala Ala Thr Asp Thr Gln Lys Gly Arg
340 345 350
Val Trp Arg Met Asn Pro Tyr Pro Cys Leu Arg Gly Ala Ile Ile Ala
355 360 365
Ala Glu Cys Glu Leu Gly Asp Val Tyr Phe Thr Leu Arg Gln Val Tyr
370 375 380
Ser Trp Ser Leu Arg Pro Glu Tyr Gly Gln Lys Glu Lys Pro Leu Glu
385 390 395 400
Val Asn Lys Tyr Val Phe Asn Arg Ile Asn Leu Phe Asp Ser Asp Leu
405 410 415
Thr Val Gly Asp Gln Ile Val His Trp Arg Tyr Glu Leu Tyr Pro Pro
420 425 430
Val Glu Thr Thr Tyr Asp Asp Gly Tyr Leu Cys Gly Ser Asp Lys Glu
435 440 445
Asp Asp Glu Leu Leu Cys Glu Val Asp Glu Glu Lys Tyr Lys Lys Met
450 455 460
Phe Glu Asn Met Ile Glu Gly Gly Trp Asp Gln Glu Arg Phe Lys Leu
465 470 475 480
His Ser Ile Leu Thr Asp Pro Asn Leu Leu Thr Ile Asp Phe Glu Lys
485 490 495
Asp Ala Tyr Leu Asn Ser Arg Ser Glu Leu Val Phe Pro Glu Tyr Phe
500 505 510
Asp Lys Trp Ile Asn Ser Pro Met Phe Asn Ala Lys Leu Arg Leu Ala
515 520 525
His Gly Glu Ile Ala Thr Arg Arg Ala Asn Asp Pro Trp Asn Asn Arg
530 535 540
Ala Val His Gly Tyr Ile Arg Thr Ser Thr Glu Ser Leu Gly Tyr Ala
545 550 555 560
Leu Gly Ser Tyr Tyr Asp Leu Arg Leu Gln Leu Phe Gly Asp Ala Leu
565 570 575
Ser Leu Glu Gln Arg Gln Ser Ala Val Phe Glu His Leu Asn Lys Gln
580 585 590
Asp Asp Phe Phe Thr Leu Thr Gly Tyr Ala Lys Asp Gln Val Val Cys
595 600 605
Pro His Ser Gly Gly Ala Phe Tyr Thr Phe Arg Arg Val Ala Leu Glu
610 615 620
Ile Leu Ser Asn Tyr Glu Lys Leu Thr Pro Asp Leu His Glu Gly Leu
625 630 635 640
Glu His Gln Thr Tyr Thr His Pro Lys Ile Asn Glu Tyr Phe Asn Arg
645 650 655
Phe Val Leu Asp Met Lys Asp Phe Ser Gln Leu Ile Cys Phe Val Phe
660 665 670
Asp His Phe Phe Glu Arg His Glu Gln Leu Arg Asp Val Ala Glu Ala
675 680 685
Arg Arg Leu Val Tyr Leu Ile Gln Lys Ser Asp Gly Pro Glu Arg Leu
690 695 700
Asp Val Leu Gln Arg Asn Phe Pro Asn Phe Phe Arg Arg Phe Met Asn
705 710 715 720
Leu Lys Asp Val Lys Tyr Ile Tyr Asp Leu Asn Val Val Asn Phe Leu
725 730 735
Pro Leu Leu Phe Leu Ile Gln Asp Asn Ile Ser Tyr Trp His Arg Gln
740 745 750
Trp Ser Val Pro Val Ile Leu Tyr Gly Asp Ile Ile Arg Leu Ile Pro
755 760 765
Val Glu Val Gly Ala Tyr Ala Asn Arg Phe Gly Met Lys Ser Phe Phe
770 775 780
Asn Phe Thr Arg Phe His Pro Gly Asp Ser Lys Lys Lys Gln Asn Ala
785 790 795 800
Asp Asp Thr His Lys Glu Phe Gly Leu Ile Cys Phe Glu Tyr Tyr Thr
805 810 815
Lys Thr Lys Ile Ser Gln Gly Gly Val Asp Ile Ser Val Val Thr Thr
820 825 830
Lys Met Asp Thr Leu Lys Val His Leu Ala Ser Leu Cys Ser Gly Ile
835 840 845
Ala Asp Ser Ile Val Tyr Thr Leu Pro Val Ala His Pro Lys Lys Cys
850 855 860
Val Val Leu Ile Val Ile Gly Asp Asp Asn Val Asp Met Gln Ile Arg
865 870 875 880
Ser Glu Gln Ala Ile Ser Arg Tyr Tyr Tyr Ser Arg Arg His Ile Ala
885 890 895
Gly Val Val Ser Ile Cys Ile Ser Gln Asp Gly Gly Leu Lys Ala Tyr
900 905 910
Ser Thr Gly Ile Val Lys His Arg Ile Cys Glu Lys Ser Ile Leu Lys
915 920 925
Tyr Lys Cys Lys Val Val Leu Val Arg Met Pro Gly Tyr Val Phe Gly
930 935 940
Asn Asp Glu Leu Met Thr Lys Leu Leu Asn Val
945 950 955
<210> 58
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV24 CAE53046的VP5
<400> 58
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Ser Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ala Ala Thr Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Thr
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Met Gln
85 90 95
Thr Lys Ile Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Lys Glu Ile Thr Lys Glu Phe Gly Lys Glu Leu Glu
115 120 125
Glu Val Tyr Asp Phe Met Asn Gly Glu Ala Lys Glu Glu Glu Val Val
130 135 140
Gln Glu Gln Tyr Ser Met Leu Cys Lys Ala Val Asp Ser Tyr Glu Lys
145 150 155 160
Ile Leu Lys Ala Glu Asp Ser Lys Met Ala Ile Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Ser Glu Arg Ser Gln Asp Glu Ile Lys Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Asn Ala Ile Glu Ile Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Met Arg Ser Pro Lys Ile Glu Pro Thr Ile Ile Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Lys Glu Ile Pro Asp Glu Gln Leu Ala Val Ser Val Leu
290 295 300
Asn Lys Lys Thr Ala Val Thr Asp Asn Cys Asn Glu Ile Ala His Ile
305 310 315 320
Lys Gln Glu Ile Leu Pro Lys Phe Lys Gln Ile Met Asp Glu Glu Arg
325 330 335
Glu Ile Glu Gly Ile Glu Asp Lys Val Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Ile Pro Arg Thr Gln Gln Pro Gln Ile His Ile Tyr Ala
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Arg Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Thr Ser His Trp His Ala Leu Gly Leu
405 410 415
Ala Gln Glu Ala Ser Gly Arg Thr Leu Thr Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ser Ile Ser Asn Thr Tyr Ser Ser Ala Ile His Ala Arg
435 440 445
Arg Met Ile Arg Ser Arg Ala Val His Pro Ile Phe Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Ala Leu Lys Asn Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Glu Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Ile
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 59
<211> 959
<212> PRT
<213> 人工序列
<220>
<223> BTV25 ACJ06702的VP2
<400> 59
Met Asp Glu Leu Val Ile Pro Ile Phe Ser Ala Asp Glu Met Pro Tyr
1 5 10 15
Ala Leu Ile Ser His Tyr Pro Leu Ala Ile Gln Thr Asn Arg Lys Ile
20 25 30
Gln Asn Asp Arg Glu Phe His Asp Leu Thr Lys Ile Pro Gln Ser Asn
35 40 45
Met Ile Asp Ile Ser Gln His Gly Val Ile Arg Ala Leu Asp Tyr Arg
50 55 60
Pro Ser Arg Asn Asp Gly Ile Val Ile Pro Arg Leu Leu Asp Ile Thr
65 70 75 80
Leu His Ala Tyr Asp Lys Arg Arg Ser Met Lys Asp Ile Cys Gly Leu
85 90 95
Glu Phe Lys Thr Asp Val Lys Trp Met Gln Trp Ala Ile Asn Asp Arg
100 105 110
Met Asp Ile Gln Pro Leu Lys Val Glu Ile Asp Asn Glu His Thr Val
115 120 125
Asn His Gln Leu Phe Asn Cys Asn Ile Lys Ile Arg Ser Lys Asn Val
130 135 140
Asp Thr Val Tyr Tyr Asp Tyr His Pro Leu Glu Ser Glu Ala Thr Lys
145 150 155 160
Cys Asn His Ala Asn Ile Glu Leu Met Arg Ser Leu Thr Met Val Glu
165 170 175
Leu Tyr His Val Leu Gln Gly Ala Ala Tyr Ala Leu Lys Thr Asn Phe
180 185 190
Glu Leu Ile Ala Thr Ala Glu Arg Gly Asp Val Arg Gln Arg Tyr Asp
195 200 205
Ala Ala Gly Val Arg Trp Ser Arg Leu Ser Arg Asp Gln Arg Ile Gly
210 215 220
Tyr Ser Gly Asn Ala Tyr Asp Met Phe Met Ala Asn Leu Val Gln Val
225 230 235 240
Glu Val Lys Gly Lys Cys Ala Asp Glu Ile Ala Gly Glu Met Gly Arg
245 250 255
Leu Ala Lys Ile Lys Asp Lys Trp Val Ser Asn Gly Phe Ser Gly Thr
260 265 270
Val Ile Phe Val Ser Glu Leu Cys Thr Glu Phe Ser Ala Ile Gly Arg
275 280 285
Lys Met Met Asp Thr His Glu Glu Pro Lys Asp Glu Glu Gly Ile Ser
290 295 300
Arg Arg Phe Gln Ile Glu Leu Asp Gly Met Phe Ser Ala Ala Arg Gln
305 310 315 320
Glu Asn Lys Asn Val Leu Ser Gln Arg Gly Ser Lys Val Thr Asp Glu
325 330 335
Ser Lys Phe Tyr Ala Leu Ile Met Ile Ala Ala Ser Asp Thr Gln Gln
340 345 350
Gly Arg Val Trp Arg Thr Asn Leu Tyr Pro Cys Leu Arg Gly Ala Leu
355 360 365
Val Ala Ala Glu Cys Val Met Gly Asp Val Tyr His Thr Leu Arg Thr
370 375 380
Val Phe Asn Trp Ser Val Arg Gly Thr Tyr Gly Gly Thr Glu Arg Asn
385 390 395 400
Leu Glu Asn Asn Arg Tyr Ile Phe Ser Arg Ile Asn Leu Phe Glu Thr
405 410 415
Asn Leu Pro Ser Gly Ser Lys Ile Val His Trp Cys Tyr Glu Glu Val
420 425 430
Ala Pro Ile Glu Thr Thr Tyr Asp Asn Gly Tyr Ile Cys Lys Ser Gln
435 440 445
Glu Gly Val Gln Asn Asp Glu Lys Leu Cys Lys Ile Asp Asp Asp Leu
450 455 460
Tyr Ala Arg Met Arg Asp Glu Ala Ile Thr Gly Gly Trp Arg Gln Glu
465 470 475 480
Asp Phe Lys Leu His Lys Ile Leu Thr Asn Pro Asn Leu Leu Thr Ile
485 490 495
Asp Phe Glu Lys Glu Ala Tyr Ile Thr Ser Thr Ser Asp Leu Ile Leu
500 505 510
Pro Asp Tyr Phe Asp Lys Trp Ile Lys Ala Pro Ile Phe Asn Ala Arg
515 520 525
Leu Arg Ile Thr Asn Gly Val Ile Glu Ser Glu Lys Lys Ser Asp Pro
530 535 540
Trp Asn Lys Arg Val Ala Asn Gly Thr Met Lys Thr Ser Ala Glu Ser
545 550 555 560
Leu Gly Tyr Val Leu Gly Arg Phe Tyr Asp Leu Arg Met Gln Phe Val
565 570 575
Glu Asp Val Leu Asn Thr Arg Gln Gln Lys Ser Leu Ala Leu Gln His
580 585 590
Ile Arg Asn Leu Glu Asp Phe Thr Arg Leu Thr Asp Tyr Gly Glu Glu
595 600 605
Gln Asn Ile Cys Pro His Ala Gly Gly Ala Met Tyr Thr Phe Arg Arg
610 615 620
Val Ala Leu Glu Ile Ile Thr Asn Tyr Glu Arg Leu Asn Pro Met Asp
625 630 635 640
His Glu Gly Asn Glu Tyr Gln Thr Tyr Arg His Pro Ala Ile Asp Tyr
645 650 655
Ser Lys Leu Gly Asp Val Leu Lys Met Lys Asp Leu Ser Gln Leu Ile
660 665 670
Cys Phe Val Phe Asp Ser Ile Phe Glu Arg Arg Asp Gln Leu Arg Glu
675 680 685
Val Gly Glu Ala Arg Met Ile Ile Tyr Gln Ile Gln Asn Ala Thr Gly
690 695 700
Glu Arg Arg Leu Asp Val Leu Lys Arg Glu Phe Pro Thr Phe Tyr Val
705 710 715 720
Trp Phe His Arg Leu Arg Asp Val Glu Leu Ile Arg Asp Leu Asn Val
725 730 735
Ile Asn Phe Leu Pro Leu Leu Phe Leu Val Arg Asp Asn Ala Ala Tyr
740 745 750
Arg His Arg Gln Trp Ser Ile Pro Met Ile Leu Tyr Asp Glu Glu Ile
755 760 765
Lys Leu Ile Pro Val Glu Val Gly Ala Tyr Ala Asn Arg Phe Gly Phe
770 775 780
Lys Ser Phe Ile Asn Phe Thr Arg Phe His Pro Gly Asp Ala Arg Lys
785 790 795 800
Arg Gln Glu Val Ser Ala Phe His Lys Ala Tyr Gly Ser Ile Cys Tyr
805 810 815
Arg Tyr Tyr Thr Asp Thr Thr Ile Ser Gln Gly Glu Val Asn Val Pro
820 825 830
Val Ile Thr Thr Lys Leu Asp Thr Leu Arg Val His Leu Ala Ala Leu
835 840 845
Cys Gly Gly Leu Ser Asp Ser Leu Val Tyr Thr Leu Pro Ile Thr His
850 855 860
Pro Glu Lys Cys Leu Val Leu Val Ile Val Gly Asp Asn Lys Leu Asn
865 870 875 880
Pro Gln Ile Arg Ser Glu Leu Val Ile Ser Arg Tyr Arg Tyr Ser Arg
885 890 895
Arg His Ile Arg Gly Val Val Ser Val Cys Ile Asp Gln Glu Gly Leu
900 905 910
Pro Asn Val Tyr Ser Thr Gly Ile Ala Arg His Lys Ile Tyr Glu Lys
915 920 925
Thr Val Leu Arg Tyr Arg Cys Lys Ile Ile Leu Val Lys Ile Ser Gly
930 935 940
Tyr Val Phe Gly Asn Asp Glu Leu Met Thr Lys Leu Leu Asn Val
945 950 955
<210> 60
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV25 ACJ06704的VP5
<400> 60
Met Gly Lys Ile Ile Arg Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Ser Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ala Thr Ile Gly
20 25 30
Arg Ala Ala Glu Arg Leu Ala Glu Ser Glu Ile Gly Ser Ala Thr Ile
35 40 45
Asp Gly Leu Leu Gln Gly Ser Val His Ser Leu Val Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Thr
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Leu Gln
85 90 95
Val Lys Leu Lys Glu Leu Glu Asp Glu Gln Arg Asn Asp Leu Val Arg
100 105 110
Leu Arg Tyr Gly Glu Gln Ile Arg Ala Lys Phe Gly Ala Glu Leu Asp
115 120 125
Glu Ile Tyr Gln Phe Met Thr Ser Ala Ile Arg Asn Asp Gly Glu Ala
130 135 140
Lys Glu Glu Tyr Glu Ile Leu Asn Lys Ala Ile Asn Ser Tyr Glu Lys
145 150 155 160
Ile Leu Lys Ala Glu Glu Thr Gln Ile Ser Thr Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Ser Glu Arg Ser Glu Asn Glu Ile Thr Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Arg Asp Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Thr Ser Glu Glu Val Pro Leu Val Gly Ala
225 230 235 240
Gly Val Ala Ala Thr Ile Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Ser Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Met Arg Thr Pro Arg Val Glu Pro Thr Thr Leu Ala Thr Thr Leu Asp
275 280 285
Arg Arg Phe Gly Glu Ile Ser Asp Lys Asp Leu Ala Ile Gly Val Leu
290 295 300
Asn Lys Arg Thr Ser Val Leu Asp Asn Tyr Ser Glu Ile Ala His Ile
305 310 315 320
Lys Gln Glu Ile Met Pro Arg Phe Lys Arg Ile Leu Asp Glu Glu Arg
325 330 335
Lys Ile Glu Gly Ala Asn Lys Lys Tyr Ile His Pro Arg Val Met Met
340 345 350
Lys Phe Lys Ile Pro Arg Thr Gln Gln Pro His Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Arg Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Ala Ser His Trp His Ala Leu Gly Ser
405 410 415
Ala Gln Glu Ala Ser Gly Arg Thr Leu Thr Glu Ala Tyr Lys Glu Phe
420 425 430
Leu Asn Leu Ser Ile Ser Ser Ala Phe Ser Ser Pro Ile His Ala Lys
435 440 445
Arg Met Ile Arg Ser Arg Thr Val His Pro Ile Tyr Leu Gly Ser Met
450 455 460
His Tyr Asp Val Thr Tyr Glu Val Leu Lys Asn Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Ile
500 505 510
Leu Gly Glu Lys Ile Asp Ile Ser Leu Phe Leu Arg Asn Ala
515 520 525
<210> 61
<211> 957
<212> PRT
<213> 人工序列
<220>
<223> BTV26的VP2
<400> 61
Met Glu Glu Phe Val Ile Pro Ile Tyr Ser Ser Arg Asp Met Pro Leu
1 5 10 15
Ala Leu Ile Ser Gln Tyr Pro Leu Ala Val Gln Val Asp Ala Ile Met
20 25 30
Ala Asp Ile Gly Asp Asp His Asn Leu Ala Leu Ile Pro Glu Leu Asn
35 40 45
Met Ile Asp Ile Ser Lys Gln Gly Ile Ile Asp Ala Leu Asp Tyr Arg
50 55 60
Pro Ala Arg Asn Asp Gly Val Val Val Pro Arg Leu Leu Asp Ile Thr
65 70 75 80
Leu His Ala Tyr Asp Cys Lys Arg Ser Met Arg Thr Ala Arg Asn Thr
85 90 95
Glu Phe Ala Thr Asp Thr Glu Trp Met Lys Trp Ala Ile Glu Asp Arg
100 105 110
Met Asp Val Gln Pro Leu Lys Ile Leu Ile Asp Asp His Tyr Phe Ile
115 120 125
Asn His Gln Leu Phe Asn Cys Asn Val Lys Ala Lys Phe Gly Asn Val
130 135 140
Asp Thr Ile Tyr Tyr Asp Leu His Pro Leu Glu Lys Lys Glu Lys Lys
145 150 155 160
Cys Asn His Met Asn Ile Glu Leu Met Arg Ser Leu Thr Met Thr Glu
165 170 175
Met Phe His Val Leu Gln Gly Ala Ala Tyr Thr Leu Lys Thr Asn Tyr
180 185 190
Thr Leu Thr Ala Thr Ala Glu Arg Gln Asp Ile Ser His Ser Phe Glu
195 200 205
Asn Val Gly Leu Arg Trp Gln Asn Leu Ala Pro Asn Thr Arg Ile Glu
210 215 220
Asn Arg Gly Ser Ala Tyr Asp Lys Phe Ile Ala Gly Leu Val Gln Val
225 230 235 240
Arg Ile Asp Gly Lys Cys Pro Asn Glu Ile Thr Ala Glu Leu Asn Gln
245 250 255
Leu Arg Ser Val Lys Glu Lys Trp Val Gly Ala Gln Tyr Gln Gly Asn
260 265 270
Ala Ile Tyr Ala His Glu Ile Cys Ser Val Met Ser Ala Ile Gly Arg
275 280 285
Lys Met Leu Asn Ala Arg Glu Glu Pro Val Asp Glu Glu Gly Ile Ser
290 295 300
Arg Arg Phe Gln Gln Glu Ile Asp Asp Val Phe Ser Gln Met Asn Arg
305 310 315 320
Glu Asn Val Ala Val Arg Arg Val Ala Val His Ala Asn Asp Glu Gln
325 330 335
Lys Phe Phe Ala Leu Met Val Ile Ala Ala Thr Asp Val Asn Gln Gly
340 345 350
Arg Ile Trp Arg Thr Asn Pro Tyr Pro Cys Leu Arg Gly Thr Leu Ile
355 360 365
Ala Ala Glu Cys Ala Leu Gly Asp Val Tyr Ser Ala Met Arg Arg Ile
370 375 380
Phe Ser Trp Ser Val Arg Pro Thr Tyr Gly Thr Gly Glu Lys Gln Leu
385 390 395 400
Glu Asn Asn Arg Tyr Val Tyr Ser Arg Ile Asn Leu Phe Glu Ser Asn
405 410 415
Ile Ser Val Asp Glu Glu Val Ile His Trp Arg Tyr Glu Leu Leu Ala
420 425 430
Pro Ala Glu Thr Thr Tyr Asp Arg Gly Tyr Ile Cys Ser Val Glu Arg
435 440 445
Gly Asp Asp Asp Leu Leu Cys Lys Ile Asp Asp Ala Ala Tyr Lys Leu
450 455 460
Met Phe Asp Glu Ala Val Arg Val Gly Trp Gln Gln His Thr Phe Lys
465 470 475 480
Leu Tyr Lys Met Leu Thr Glu Pro Asn Leu Leu Thr Ile Asp Phe Glu
485 490 495
Lys Asp Ala Tyr Leu Gly Pro Asp Ser Ser Leu Val Met Pro Asp Tyr
500 505 510
Tyr Asn Lys Trp Ile Arg Ser Pro Met Phe Asn Ala Arg Leu Arg Leu
515 520 525
Thr Lys Ala Arg Ile Asn Ala Glu Lys Thr Gly Asp Pro Trp Asn Asp
530 535 540
Arg Val Val Gly Gly Thr Ile Lys Thr Pro Val Glu Ser Leu Gly Tyr
545 550 555 560
Thr Leu Gly Arg Phe Tyr Asp Leu Arg Leu Gln Phe Val Glu Asp Val
565 570 575
Leu Ser Tyr Arg Gln Gln Gln Ser Ala Ile Phe Gln Tyr Met Gly Ala
580 585 590
Arg Glu Asp Phe Ala Met Leu Thr His Tyr Ala Arg Gly Glu Arg Asp
595 600 605
Ile Cys Pro His Ala Gly Gly Thr Leu Tyr Ile Thr Arg Arg Val Ala
610 615 620
Leu Glu Val Leu Thr Ser Tyr Glu Arg Leu Asp Pro Gln Tyr His His
625 630 635 640
Gly Arg Glu His Leu Thr Tyr Cys His Pro Ser Ile Asp Tyr Thr Arg
645 650 655
Arg Glu Arg Ile Phe Glu Ile Glu Asp Leu Ala Gln Leu Ile Cys Tyr
660 665 670
Val Ile Asp Tyr Ile Phe Glu Lys Arg Asp Ser Leu Arg Glu Val Gly
675 680 685
Glu Ala Arg Arg Ile Val Tyr Leu Ile Gln Ser Val Thr Gly Ile Arg
690 695 700
Arg His Glu Val Leu Asn Gln Ala Phe Pro Arg Phe Phe Ala Trp Phe
705 710 715 720
Leu Arg Leu Lys Asn Val Glu Val Ile Arg Asp Leu Asn Ile Val Asn
725 730 735
Phe Leu Pro Leu Leu Phe Leu Val Arg Asp Asn Ile Ser Tyr Gln His
740 745 750
Arg Gln Trp Ser Val Pro Met Ile Leu Tyr Asp Gln Thr Ile Lys Leu
755 760 765
Ile Pro Val Glu Val Gly Ala Tyr Ala Asn Arg Val Gly Phe Lys Ser
770 775 780
Phe Phe Asn Phe Val Gln Phe His Pro Gly Asp Ala Gln Lys Arg Gln
785 790 795 800
Glu Ala Asp Glu Ser His Lys Lys Met Gly Gln Ile Cys Tyr Ala Tyr
805 810 815
Tyr Thr Asp Thr Thr Ile Ser Gln Gly Lys Val Asp Thr Pro Val Ile
820 825 830
Ala Thr Lys Met Asp Thr Leu Arg Val His Leu Ala Ala Val Cys Ala
835 840 845
Gly Leu Ser Asp Ser Leu Val Tyr Ala Leu Pro Val Ala His Pro Glu
850 855 860
Arg Cys Ile Val Leu Ile Ile Ile Gly Asp Glu Lys Leu Asp Pro Asn
865 870 875 880
Val Arg Thr Glu Leu Val Val Ser Arg Tyr Arg Tyr Ser Arg Lys His
885 890 895
Ile Arg Gly Ala Val Ser Ile Cys Met Tyr Lys Asn Gly Gln Phe Arg
900 905 910
Val His Thr Thr Gly Ile Val Arg His Arg Ile Cys Glu Lys Thr Ile
915 920 925
Leu Asn Phe Arg Cys Arg Ala Ile Leu Val Arg Thr Pro Gly Tyr Val
930 935 940
Phe Gly Asn Asp Glu Leu Met Thr Lys Leu Leu Asn Val
945 950 955
<210> 62
<211> 523
<212> PRT
<213> 人工序列
<220>
<223> BTV26 AER60536的VP5
<400> 62
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Ile Gly
1 5 10 15
Ser Ala Leu Thr Ser Asn Ala Ala Lys Lys Ile Tyr Ala Thr Val Gly
20 25 30
Lys Ala Ala Glu Arg Leu Ala Thr Ser Glu Leu Gly Thr Ala Ala Ile
35 40 45
Asp Gly Leu Leu Gln Gly Ser Val His Ser Met Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Thr
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Met Gln
85 90 95
Met Lys Ile Gln Glu Leu Glu Asp Glu Gln Arg Ser Glu Leu Ile Arg
100 105 110
Leu Lys Tyr Asp Asp Glu Ile Thr Arg Ala Phe Gly Asn Glu Leu Arg
115 120 125
Glu Val Tyr Gln Phe Met Asn Gly Gln Val Arg Ala Asp Leu Asp Gln
130 135 140
Tyr Asp Val Leu Arg Lys Ala Val Asp Ser Tyr Glu Lys Leu Leu Asp
145 150 155 160
Ala Glu Asp Ala Lys Met Ala Ile Leu Ala Arg Ala Leu Gln Lys Glu
165 170 175
Met Arg Glu Arg Ser Ala Glu Glu Ala Lys Met Val Arg Glu Tyr Arg
180 185 190
Ser Lys Ile Asp Ala Leu Lys Glu Ala Ile Glu Val Glu Arg Asp Gly
195 200 205
Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr Ala Asp Val
210 215 220
Leu Glu Ala Thr Ala Glu Glu Val Pro Leu Ile Gly Ala Gly Ala Ala
225 230 235 240
Ala Ala Ile Ala Ser Gly Arg Ala Ile Glu Gly Ala Tyr Lys Leu Lys
245 250 255
Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His Met Arg Thr
260 265 270
Pro Lys Ile Glu Pro Thr Val Ile Ser Thr Val Leu Glu His Arg Phe
275 280 285
Lys Ser Ile Pro Asp Asn Leu Leu Ala Asn Gly Val Leu Ser Lys Lys
290 295 300
Ile Ser Ile Met Asp Asn Gln Asn Glu Ile Arg His Ile Lys Arg Glu
305 310 315 320
Ile Leu Pro Arg Phe Lys Arg Ile Met Asp Glu Glu Arg Ala Ala Ala
325 330 335
Asn Arg Glu His Lys Val Ile His Pro Thr Val Met Met Lys Phe Lys
340 345 350
Val Pro Arg Ala Gln Gln Pro Gln Ile His Ile Tyr Ala Ala Pro Trp
355 360 365
Asp Ser Asp Glu Val Phe Phe Phe His Cys Val Ser His His His Arg
370 375 380
Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp Ile Val His
385 390 395 400
Phe Glu Asp Leu Thr Asn His Trp His Ala Leu Gly Val Ala Gln Glu
405 410 415
Ala Lys Gly Arg Thr Leu Gly Glu Ala Tyr Lys Glu Phe Leu Asn Leu
420 425 430
Pro Ile Ser Ser Ser Phe Ser Ser Arg Ile His Ala Arg Arg Met Val
435 440 445
Arg Ser Arg Leu Val His Pro Ile Phe Leu Gly Ser Met His Tyr Asp
450 455 460
Val Thr Tyr Glu Thr Leu Lys Asn Asn Ala Gln Arg Ile Val Tyr Asp
465 470 475 480
Glu Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His Phe Gln Arg
485 490 495
Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Ile Leu Gly Asp
500 505 510
Lys Ile Asp Ile Pro Leu Phe Leu Arg Asn Ala
515 520
<210> 63
<211> 3944
<212> DNA
<213> 人工序列
<220>
<223> BTV1的VP1 DNA
<400> 63
gttaaaatgc aatggtcgca atcaccgtgc aaggtgcaca gctcatcaag cgagtggtcg 60
aacgctttta tccagggata gcatttaata tagatgaagg agcatgttat atatataagt 120
tttctgatca tatacgacgc ataaggatga aacatggaac gaaatatcga cggcaggcgg 180
aagagattat gcgcaatata agcttgagga aagagcgatt gtatgggata ccagtattag 240
atgaggttga gtggaagtat gtgtttgatg gtcaaacgtt ccaaagttac gcttttgagg 300
tgtacgtgaa ctcaattttg ccgtggagtg aacttgatcc ggaggaagag ttcttacgta 360
attatagagt ttcaagggag atgactgaag tggaaaaatt tatcgaattc cgtgctaaaa 420
acgagatgca aatatacgga gatataccca ttaaggtatg gtgttgtttc atcaatgaac 480
tgagtgcgga attaaaacat attcccttag ggatgcaagt tatggctgac tttgtaaacc 540
gtttcgattc accattccat caggggaata gagatttatc aaatcttgaa gattttcaag 600
ttgcatacac tacgccgctt ttgtttgaaa tgtgttgcat ggaatcaatt ttagaattca 660
atatcaaaat gcgtatgcgt gaagaagata tctcggcgct ggaattcggt gatatgaaag 720
ttgatccggt tggactattg cgtgagtttt tcattctgtg cttaccacac ccaaagaaga 780
ttaacaacgt tctaagagca ccatactctt ggtttgtaaa gatgtggggc gtcggagctg 840
atccgatcgt tgttttacaa tctacggcag gcgatgacag gaattcaaag gatgtgtttt 900
atgacaagtt tcgaaccgag ccaaatcgat acaaagccct atttcggtca tcgttttata 960
acgaatcaag acgaatgaat gaggagaaga ttctagaggc agtgaagtat tcgcaaaaat 1020
tgggctcgca cgaacgtaga ttacctcttt ttgagaaaat gttaaagacg gtttatacta 1080
caccatttta tccacataag agctcgaata tgatactagc gtcctttcta ttaagtattc 1140
aaactattac tggatatggc agggcgtggg tgaagaacgt gagtactgag ttcgataaac 1200
agctgaagcc gaacccgagc aatctagttc aggatgtttc ggatttaacg cgggaattct 1260
ttaaacaagc atatgttgaa gcaaaagaac gtagagaaga gatcgttaaa cctgaggatt 1320
tatatacatc gatgttgcga ttagcgagaa atacaagttc aggtttttca accgagattt 1380
atgtaaaaaa gagatttggt ccaagactaa gggataaaga tttgatcaag atcaattcaa 1440
ggattaaggc tttagttatt ttcactaaag gacatactgt tttcactgat gaagaacttc 1500
ataaaaaata taatagtgta gaactatacc aaactaaggg ttcaagagat gtaccgatta 1560
aggctacgag aacgatatat tcaatcaatc tttcagtgtt ggtaccgcag ttaattgtta 1620
ctttaccctt gaatgaatat ttttctaggg ttggcgggat aaccagtccg gattataaga 1680
agatcggagg aaaagtaatt gtcggagatt tggaggccac ggggtcgcgc gtgatggatg 1740
ctgctgattg ctttcgtaac tctgcagacc gcgatatatt cacaattgca atcgactata 1800
gtgaatatga tacacaccta acgcgacata attttcgaac cggtatgctc caaggaatca 1860
gagaagccat ggccccctat agggatttgc gatatgaagg ttatacgttg gagcaaatca 1920
tagattttgg atatggagag gggagagtag cgaatacgtt gtggaatgga aagcgaagac 1980
tgtttaaaac tacatttgat gcgtatatac gattagacga gagcgagcga gacaaaggta 2040
gtttcaaagt ccccaaagga gtgcttccag tatcgagtat tgatgtcgcg aaccgaatcg 2100
cggttgacaa aggattcgat acgcttatcg cggcaacgga tggaagcgat ttggctttga 2160
tcgacacgca cctttccggc gagaattcga ctctcatcgc taactcgatg cacaacatgg 2220
ctattgggac cttgatacaa cgagaagttg ggaaggagca gccagggatt cttactttct 2280
tatcggagca atacgtggga gatgacacac tgttttacac aaagctacac actacagata 2340
ctaaggtttt tgataaagtg gcggcttcaa tttttaatac agtggcgaag tgtggacatg 2400
aagcttcacc tagtaagaca atgatgacgc catattctgt ggagaagacc caaacgcatg 2460
caaagcaggg ctgttacgta ccgcaagatc gtatgatgat tatctcatca gaaaggagga 2520
aggatatcga ggatgtgcag gggtacgtgc gttcgcaagt gcaaacgatg ataacgaagg 2580
tgagcagagg attttgtcat gatttggcgc agctaatatt aatgcttaag actactttca 2640
tcggtgcgtg gaaaatgaag cgaactatta aggaagatgc gatgtatcgc gacagaaagt 2700
ttgattcgaa cgatgaggat gggtttacgt tgatacagat tcgaaatccc ttagcattat 2760
atgtccctat aggctggaat gggtacggcg cgcatccggc agctcttaat atcgttatga 2820
cggaagagat gtatgttgat tcgatcatga tctcaaaact ggatgaaatc atggcgccga 2880
taaggaggat tgtgcatgac attccaccgt gttggaatga gactcaggga gacaagcgcg 2940
gattgattag tgcaactaaa atgagtttct tttcgaagat ggctaggcct gctgtccaag 3000
ccgctttaag cgatccgcag ataatgaatc tagttgaaga gcttccactt ggggagtttt 3060
cacctggacg tatctcgaga accatgatgc atagtgctct tctgaaggaa tcgagcgcta 3120
ggacgttgtt atccagtggt tacgaactag aatatcagaa ggctttgaat agttggatcg 3180
cacaagtttc gatgcgtttg ggagaggagt caggagtaat atcaacatct tatgcgaaac 3240
tctttgacgt atacttcgaa ggtgagttag acggcgcacc ctatatgttt ccagaccaga 3300
acttatctcc gcagttctat atacagaaga tgatgattgg cccacgagtt agttcacgag 3360
tgcggaattc ttatgtagat cggattgatg tgatattgag gaaggatgtc gttatgcgcg 3420
gctttatcac tgccaatacc attctgaacg taattgagaa actgggaact aatcactcag 3480
tgggagattt agtcacagtt tttacgctta tgaacatcga gacacgtgtg gccgaagagc 3540
tagctgaata tatgacttca gaaaagatac gatttgacgc gttgaagctt ctaaagaaag 3600
gaatcgctgg cgacgaattt actatgtcgt tgaacgttgc tacacaggat tttattgaca 3660
cttacctcgc ctatccctat cagttgacga aaacggaagt cgacgccata tcgttgtatt 3720
gcacgcagat ggttatgcta cgcgcggcgc tcgggttacc aaagaagaag atgaagatcg 3780
ttgtaactga tgatgcaaag aaaagataca agatacgttt gcagaggttt agaacgcacg 3840
tacctaaaat taaagttctt aagaagttga ttgatccaaa tagaatgaca gttagaaatc 3900
ttgaaaacca attcgtttga gagcacgcgc cgcattacac ttac 3944
<210> 64
<211> 2940
<212> DNA
<213> 人工序列
<220>
<223> BTV1的VP2 DNA
<400> 64
gttaaaatag tagcgcgatg gatgaactag gcatcccagt ttataagaga ggatttcccg 60
aacacctgct tcgtggttac gagttcataa tagatgttgg aaccaagata gaaagtgttg 120
gaggacgtca tgatgtaacg aaaataccag aaatgaatgc atatgacatc aagcaggaga 180
gcatccgaac cgcattatgg tataatccga taagaaatga tggttttgta ttgccgcgag 240
tgctggatat cacattgagg ggttacgatg aaagacgggc ggttgttgaa agtacgagac 300
acaagagttt ccatacgaat gaccagtggg tgcagtggat gatgaaagat tcgatggacg 360
ctcagccttt aaaggttggg ttagatgatc aaagtaggaa tgtagctcac tcgttacata 420
actgcgtagt caaaatcgat tcaaagaagg ctgatactat gtcttaccat gtagagccga 480
tagaggacgc gtcaaaggga tgtttgcata cgagaaccat gatgtggaac cacctggtac 540
gaatagaaac atttcatgcg gcgcaggagg tggcatatac tcttaaacct acttatgaca 600
tcgtggtcca cgctgaaagg agagatcgta gtcaaccgtt taggccaggg gatcagacat 660
taattaattt tgggagaggt cagaaggtgg caatgaacca caattcatat gataagatgg 720
ttgagggatt aacacattta gtgattagag ggaaaactcc tgaggtgatt agagatgata 780
ttgcgagctt ggatgagata tgtaataggt ggatacagag taggcacgac cccggagaga 840
taaaagcata tgaattatgt aaaatattat caacgattgg tcggaaagtc ctcgatcgag 900
agaaagaacc agaggacgag gcgaatctat cgatccgatt tcaagaggca atcgacaata 960
agttccgaca acatgatcct gagcgcctga agatatttga gcataggaat caacgtagag 1020
atgaggaccg attctatatt ctgttgatga ttgcagcctc cgatactttt aatacacgag 1080
tgtggtggtc gaacccatat ccatgtttaa gaggaacctt aatcgcgtca gaaacgaagc 1140
taggtgacgt ttattcgatg atgcgttcat ggtatgattg gagtgttcga ccaacctata 1200
cgccttacga gaaaacgaga gaacaggaaa aatatattta tggacgggtt aacctgtttg 1260
actttgtcgc ggaacctggg attaaaattg ttcattggga atataggctg aatcattcca 1320
cccgggagat aacctatgcg caagggaacc catgtgattt atacccagag gatgatgatg 1380
taatagtcac aaagttcgat gatgtcgcgt atggccaaat gatcaatgag atgataaatg 1440
ggggttggaa tcaagaacag ttcaagatgc ataaaatttt aaaatcggaa ggtaatgttc 1500
taacgataga ttttgaaaaa gatgcaaagc taacaaccaa tgaaggcgta acgatgccag 1560
aatatttcaa taagtggata atcgctccga tgttcaacgc taagctgcgt ataaaacatg 1620
aagagattgc gcagcgtcaa agtgatgatc cgatggtaaa acgtacttta tcacctatta 1680
ccgcagatcc aatcgaattg caaaggttga ctttggcgcg gttttacgac attcgtcccg 1740
ctttaagagg acaggcgctt tcgcggcaac aggcacagtc cacttacgat gaagaaatat 1800
cgaaacagcg ggattatgcg gaaatattga aacgtcgtgg aattgtgcaa atcccaaaga 1860
aaccttgccc aactgtgaca gctcagtata cgctggagcg ttatgctttg ttcattataa 1920
acatcttaca acagcatgta gcacgagact gcgacgagga ggcgatatac gagcatccga 1980
aagcagatta tgaacttgaa atatttggcg agagcattgt ggatatctct caagtgatcg 2040
ttctagtttt tgacttgata tttgagagaa gaaggagggt aagagatgtg tatgaatcgc 2100
ggtacataat tgcgcgcatt aggaggatgc gaggtaaaga gagattgaac gtgatcgcgg 2160
agtttttccc aacctatggg agtcttctaa atggattaaa cagcgctacc gtagtacagg 2220
atattatgta tttgaacttt ctcccattgt attttttagc aggcgataac atgatatact 2280
ctcataggca gtggtctatt cctttacttc tatacactca tgaagtgatg gtggtcccat 2340
tagaagttgg ttcatacaat gatcggtgcg gattaattgc gtacctggaa tacatggttt 2400
tctttccctc caaggcgatt cgacttagca aactaaatga agcgcagccc aagattgcgc 2460
gcgagatgct taagtactac gctaatactg cggtatatga tgggggagtc aactacaacg 2520
ttgtgacgac gaagcagctt ctatatgaga cataccttgc ttcgttatgt gggggtattt 2580
ctgacggtat tgtctggtat ttgccgatca cacatccgaa caaatgcatc gtagcgatcg 2640
aggtatctga tgaaagggtt ccggctagca ttagagcggg gcgtataagg ctaagatttc 2700
cgctaagcgc gcgacatcta aagggggttg taatcataca aattgacgag gagggcgaat 2760
ttacagtgta tagcgaggga attgtgtctc atcgggtgtg taaaaagaat ttactcaagt 2820
atatgtgcga tattatatta ctgaagtttt cggggcacgt ttttggtaac gacgagatgc 2880
tgacaaaact tctcaacgta tgatctgctt gaccgcagat ccgcgcacta ttagacttac 2940
<210> 65
<211> 2772
<212> DNA
<213> 人工序列
<220>
<223> BTV1的VP3 DNA
<400> 65
gttaaatttc cgtagccatg gctgctcaga atgagcaacg tccggaacga ataaaaacga 60
caccgtattt agagggagat gtgctttcga gtgattcagg accgctgctt tctgtgttcg 120
cgttgcaaga gataatgcag aaagtgagac aagtgcaagc tgattatatg actgcgacgc 180
gagaggttga tttcacagta ccggatgtac aaaagattct agatgacatt aaagcgttag 240
ccgcagaaca ggtgtataaa attgtcaaag ttcccagtat ttcgttcaga catatcgtaa 300
tgcagtcaag agatcgagtc ttacgagtag atacctacta tgaagagatg tcgcaggttg 360
gagatgttat aacggaagat gagccagaaa aattctattc aaccataatt aagaaggtac 420
ggttcatacg cggaaaagga tcctttatat tgcatgatat tccgaccaga gatcatcgcg 480
gtatggaagt tgcagaacca gaagtgttgg gagttgaatt taaaaatgtg ttacctgtgt 540
tgaccgccga gcatcgcgcg atgattcaga acgcattgga tggatcgata atcgaaaatg 600
gaaacgtagc cacacgagat gtcgacgtat tcataggtgc ttgttctgaa ccgatctatc 660
gcatatataa taggttgcaa gggtatattg aggcggtgca gttacaagag ttaaggaact 720
caattggttg gttggaaagg ttaggacaga ggaaaaggat tacatattca caagaagttc 780
taactgactt tagaagacag gacacgattt gggttttagc tttacagcta ccggttaatc 840
cgcaagtagt gtgggatgtg ccacgcagtt ctattgccaa cttaatcatg aatatagcaa 900
cgtgtttacc tacaggagaa tacatcgcac caaacccaag gatttcatcg attacgttga 960
ctcaaagaat tacgacaacg gggccgttcg ctattctgac cgggtcgacc ccaactgcgc 1020
agcaacttaa tgatgttagg aagatctact tagcgctaat gtttcctgga cagattatac 1080
ttgatctaaa aattgatccc ggtgagagga tggatccggc agtaaggatg gtcgccggtg 1140
ttgtaggtca tttactcttt acagcaggtg gaagatttac aaatctgaca cagaatatgg 1200
cgagacagct tgatatagct ttaaacgatt atctacttta tatgtacaac actagagtcc 1260
aagtcaatta tggtccaaca ggtgaaccgt tagatttcca gattggaagg aatcaatatg 1320
actgtaatgt tttcagagca gatttcgcga caggaacagg atataatgga tgggctacaa 1380
tagatgttga gtatagagat ccagcccctt atgtgcatgc acaacgctac atacgttatt 1440
gtggcattga ttcgcgtgag ttgatcaatc caacaacata tggcattggg atgacttatc 1500
attgctacaa cgagatgttg cgaatgctag ttgctgcagg gaaagattct gaagcggcgt 1560
actttcggag catgttaccc tttcatatgg taaggttcgc tagaataaac caaatcataa 1620
atgaagattt acactctgtg ttctcgttgc cagacgatat gttcaacgca ttattacccg 1680
acctaatcgc cggggcgcat cagaacgccg acccagtcgt gctagatgtg agctggatat 1740
cgctgtggtt tgctttcaac agatcatttg agcctacgca tagaaatgag atgctcgaga 1800
ttgctccgct gatcgaatcc gtttatgcgt ctgagttatc cgtgatgaag gtagatatgc 1860
gacacttatc attgatgcag agaaggtttc cagatgtttt aatccaagcg aggccatctc 1920
acttctggaa ggcagttcta aatgatagtc cagaggcggt gaaagcagtt atgaacttgt 1980
cgcattcgca taatttcatc aatataagag atatgatgcg ttgggtgttg ctcccgtcgc 2040
tgcagccatc gttaaagctc gttttggaag aggaggcatg ggctgctgca aacgatttcg 2100
aagatctaat gcttactgat caagtttata tgcaccgaga tatgttgccg gagccacggt 2160
tggatgatat tgagaggttt aggcaggagg gcttctacta cacgaatatg ttagaagcgc 2220
caccggagat agatcgtgtg gtccagtata cttatgaaat cgcacgtctg caggcaaaca 2280
tggggcaatt ccgagcggct ttaagacgta ttatggatga tgatgactgg gtaaggtttg 2340
gcggtgtgct acgcactgta cgcgttaaat tcttcgatgc gcgaccccca gacgatattt 2400
tgcaaggctt gcctttcagt tatgatacga atgagaaggg tggattatca tacgcgacaa 2460
tcaagtatgc tactgagacc acaatttttt atctgatata taatgttgag ttctcaaata 2520
cacccgactc cttggtattg ataaacccga catatacgat gactaaagtc ttcattaata 2580
agagaatcgt tgagcgagta cgggttggac agattctggc tgtattgaac agaagatttg 2640
tggcatataa aggaaaaatg aggatcatgg acatcaccca atcgctcaag atgggcacca 2700
agctggctgc gccaactgtg taggtgcgcg accaatctat gcacttggta gcggcagcgg 2760
gaacacactt ac 2772
<210> 66
<211> 1981
<212> DNA
<213> 人工序列
<220>
<223> BTV1的VP4 DNA
<400> 66
gttaaaacat gcctgagcca cacgcagtac tctatgtcac aaacgagctc tctcatatcg 60
tcaagagtgg atttttgcca atatggagat taactggaga tgagagtctg aacgatttat 120
ggcttgaaaa tggaaaatat gcgacagatg tatatgcata cggagatgta tcaaaatgga 180
cgatacgaca actacgaggt catgggttca ttttcgtaag tacacacaag aatatacagc 240
tggctgatat cattaaaacg gtcgatgttc gtattcctcg ggaagtcgca aaaagtcaag 300
atatgaaagc atttgaaaat gaaattggta gacgcagaat ccgtatgcgt aaaggatttg 360
gtgatgcgct gaggaattac gcctttaaga tggctatcga atttcacggg tcggaagcgg 420
agacgttgaa cgatgcaaat cctcgattgc ataaagttta cggaatgcca gagataccgc 480
cgctatacat ggaatatgcg gagattggaa ctagatttga cgatgagcca actgatgaaa 540
agttagtatc gatgcttgat tatatcgttt acagcgcaga ggaggtgcac tacgttggat 600
gcggtgactt acgtaccttg atgcagttta aaaaacgttc accaggacgg tttagaagag 660
tattatggca cgtatatgat ccaatagcgc cagaatcttc agatccaaac gttatagttc 720
ataacgttat ggtagattca aagaaagaca ttttgaaaca tatgaacttt ttaaaacgtg 780
ttgaaagact tttcatatgg gatgtgtcat cagatcgatc gcagatggat gatgacgaat 840
gggaatcgac cagattcgca gaggacaggt tgggtgagga gatagcttat gagatgggtg 900
gtgcgttttc cagtgcattg attaaacata ggataccaaa ttcaagagac gagtatcact 960
gcatttcgac ttaccttctc ccccagccgg gggctgatgc ggatatgtac gaattaagaa 1020
attttatgag attgaaaggt tactcacatg tagatcgcca catgcatcca gatgcctccg 1080
tgatgaaagt cgtttcgcgt gatgtacgca aaatggtcga attgtatcat ggccgcgatc 1140
gtggcagatt tctaaagaaa aggctatttg agcacctcca tatcgtacgt aagaatgggt 1200
tgctgcatga aagtgatgaa ccacgagcag atctgtttta tttgaccaat cggtgtaaca 1260
tgggattgga accaagcatt tatgaagtaa tgaagaagtc ggtgatcgct acagcctggg 1320
taggccgtgc tcctctatat gattatgatg attacgcgtt accccgatct actgtcatgc 1380
tcaatggatc ttaccgtgat atcagagttc tagatggtaa tggcgcaatc ctatttctaa 1440
tgtggaagta cccagatatt gtgaagaaag atttaacata tgatccagcg tgggcgatga 1500
attttgccgt ctcgttaaag gagccgatac ctgacccccc tgtgcccgat atctccctat 1560
gtagattcat cggactgcgc gttgagtcat ctgtgttgag ggtccgaaac ccaacattac 1620
atgaaacggc tgatgaacta aagcggatgg gattggattt gtctggtcat ctatacgtca 1680
cattgatgtc cggcgcttat gtcacagatc tgttctggtg gtttaagatg attctcgact 1740
ggtctgcgca aagcaaggaa caaaaattac gtgatttaaa gaggtctgca gcggaggtaa 1800
tcgaatggaa ggagcagatg gccgagcgtc catggcacgt gagaaatgat ctaattgcag 1860
cgctgaggga gtacaaacgg aaaatgggga tgagagaggg agcctcgatc gattcgtggc 1920
tagaattact gcgtcactta taatgcgtga ctgctaggtg aggggggcat gtacaactta 1980
c 1981
<210> 67
<211> 1635
<212> DNA
<213> 人工序列
<220>
<223> BTV1的VP5 DNA
<400> 67
gttaaaaagt gcgcccttag cgaagatggg taaagtcata cggtccttaa accgatttgg 60
caaaaaggta ggcaacgcgt taacttctaa taccgcaaaa aagatctata gtacaattgg 120
aaaagcggcg gagcgatttg ctgagagtga gataggttca gcggcgatcg atggattagt 180
acagggaagc gtacattcaa tcataacggg cgaatcttac ggcgaatctg tgaaacaagc 240
tgtgttgtta aatgtgttgg ggagtggtga ggaaattcct gatccgctaa gtccaggaga 300
gcgagggata caagctaagt tgaaagagtt agaggacgag caacgcaatg aattagttcg 360
cttaaaatat aatgataaga ttaaggaaaa atttggaaaa gagcttgaag aggtttacaa 420
ttttatgaat ggagaggcga atgctgagat tgaagatgag aagcagtttg atatactgaa 480
caaggcggtg acctcgtata acaaaattct tacggaggaa gatctacaga tgcgccggtt 540
agctacggcg ctgcagaaag agatcggaga aagaacacat gcggagacgg tcatggtgaa 600
agaataccgc gataaaattg acgctttaaa aaatgcgatt gaggtagaaa gagatggcat 660
gcaagaggag gcgatacagg agatcgcggg gatgaccgca gatgtgttag aggcggcatc 720
ggaggaggtt ccgctgattg gtgcggggat ggccacagct gtggcgacag ggagggctat 780
tgagggagcg tacaaactca aaaaagtgat caatgcttta agcgggatcg atttaacgca 840
tttacgcact ccgaaaatcg aacctagtgt tgtttcaact attcttgagt accgcgcaaa 900
ggagattcct gataacgctc tggctgttag tgttctatca aagaatcgtg cgattcaaga 960
aaaccacaaa gaactgatgc atattaagaa tgagatatta ccgagattta agaaagcgat 1020
ggatgaagaa aaggaaatat gcgggataga agacaaagtg atccacccga aggtcatgat 1080
gaagtttaag attccaagag ctcaacagcc gcagattcat gtatacagtg ccccatggga 1140
ttctgatgat gtgtttttct ttcattgcat ctcacaccat catgcaaatg agtcgttctt 1200
cttaggtttc gatttgagca ttgacttagt tcattatgaa gatctcaccg cccattggca 1260
tgcattggga gcagctcaaa cggcagcggg acgtacgcta actgaagcat atagagagtt 1320
tctaaatttg gcgatttcaa atgcatttgg cacgcaaatg cacacgagaa gattggttag 1380
gtcgaaaacg gtacatccga tttacttagg ttccttgcac tacgatattt ccttttcgga 1440
tctgcgtgga aacgctcaga gaattgttta tgacgatgaa ctgcagatgc acatacttcg 1500
tgggccgata cattttcaga gacgggcaat actaggggct ttgaaatttg gatgtaaggt 1560
tttgggggac cgtttagacg taccactctt cttacgaaat gcttgaacgc agcgacggga 1620
agcacttaca cttac 1635
<210> 68
<211> 1772
<212> DNA
<213> 人工序列
<220>
<223> BTV1的NS1 DNA
<400> 68
gttaaaaaag ttctctagtt ggcaaccacc aaacatggag cgctttttga gaaaatacaa 60
catcagtggg gattacgcaa atgccacgag aacttttttg gctatttcac cacaatggac 120
ttgcagtcat ctaaaaagga attgtttatt caatgggatg tgtgttaaac aaaattttga 180
gagagcaatg attgcggcaa ccgacgtgga ggagccggcg aaagcatata aattagttga 240
attggcaaag gaggcaatgt atgatcggga gacagtctgg ctccaatgct tcaaaagctt 300
ttcccaacca tacgaagagg atgtcgaagg gaagatgaag cgatgcgggg tgcaactgct 360
cgaagattac cgcaaaagtg ggatgatgga tgaggccgtg aagcaatccg cattggttaa 420
ttcggaaaga gttagattgg atgattctct ttctgcaatg ccttacatct atgtgccaat 480
taaagagggt caaatcgtga atccaacatt tatatcaaga tatcgccaaa ttgcatacta 540
tttctacaac ccggatgcgg ctgatgattg gatcgatcca aacttgtttg gtattcgcgg 600
acagcacaat caaatcaaac gtgaggttga gagacaaatt aacacatgtc cctacactgg 660
atacaaaggc agagtatttc aagtgatgtt cttgccgatt cagctgatca atttcttaag 720
gatggatgat tttgcgaaac attttaacag gtatgcttcg atggcgatac agcaatattt 780
gagagttggt tatgctgaag aggtcagata tgtgcaacaa ctttttggaa gggttccaac 840
gggcgaattt ccattacatc aaatgatgct gataagacgc gatttcccaa cgcgcgatcg 900
cagcattgtg gaagcgcggg tgaggagatc gggtgatgag aactggcaaa gctggctgct 960
acctatgatt attgttcgtg aaggattgga tcacccggat cggtgggaat ggcttattga 1020
ttatatggat agaaagcata catgtcagct gtgctacttg aaacactcaa agcagatccc 1080
gacctgtggt gtgattgatg tacgcgcatc agaactaact gggtgttcgc cattcaagac 1140
ggtgaagatt gaggaacatg tgggaaatga ttcggttttt aagacaaaat tagttcgcga 1200
tgaacaaatt ggcaggatcg gagatcatta ttatactaca aattgttaca ctggggcgga 1260
ggccttgatt acaactgcga ttcacattca ccgctggatt agaggctgtg gcatctggaa 1320
cgatgaggga tggcaggagg gtattttcat gctcggacgc gtactactga gatgggagct 1380
gacgaaggcg cagcgcagcg ctctgctcag gttattctgc tttgtgtgct acggatatgc 1440
gccgcgtgca gatgggacga taccggattg gaataacctt ggaagctttt tggatatcat 1500
tttgaaagga ccggagctca gcgaggatga ggacgagagg gcttatgcta cgatgtttga 1560
aatggttcga tgcattatca ccctatgcta tgcagaaaag gttcacttcg ctgggttcgc 1620
ggcacctgcg tgtgagagcg gggaagttat caatctcgct gctcgtatgt ctcagatgtg 1680
gatggagtat tagttactga cttctgtttt ctgtttcttc ttttttcttc ttctattttc 1740
tcttagcact ctactagaac ttttcaactt ac 1772
<210> 69
<211> 1156
<212> DNA
<213> 人工序列
<220>
<223> BTV1的VP7 DNA
<400> 69
gttaaaaatc tatagagatg gacactatcg ctgcaagagc acttactgtg atgcgagcat 60
gtgctacgct tcaagaagca agaattgtgt tggaagctaa cgtgatggag atactgggga 120
tagcaatcaa cagatataat ggattaactt tacgaggggt gacgatgcgc ccgacctcat 180
tggcgcagag aaatgagatg ttttttatgt gtttagatat gatgctgtcc gcggctggga 240
taaacgtagg accgatatct ccagattata cccaacatat ggctacaatt ggtgtactag 300
cgacgccaga gatacctttt acaacggaag cggcgaatga gattgctcgc gtgactgggg 360
agacttcgac gtggggacca gcgcgtcagc cctatggttt tttccttgaa actgaagaga 420
ctttccaacc cgggagatgg ttcatgcgtg ccgctcaagc agtaactgcg gtagtgtgtg 480
gtccggatat gattcaagtg tcactgaatg ctggagcaag aggagatgtg cagcagatat 540
ttcagggtcg taacgacccc atgatgatat atctagtttg gagaagaatt gaaaacttcg 600
cgatggcgca gggtaactca cagcaaactc aagcaggcgt gactgttagc gttggtggag 660
tagatatgcg ggcggggcgt atcatagcgt gggatggaca ggctgctcta catgtgcaca 720
atccaacaca acagaatgcg atggttcaga tacaagtcgt gttctacatt tctatggata 780
agaccttaaa tcaataccct gccttgactg ctgaaatctt taatgtttat agcttcagag 840
atcacacatg gcacgggttg aggacggcta tactgaacag aactacactg ccgaatatgc 900
tgccacccat ctttccacca aacgatcgag atagtatcct gactcttttg cttttgtcta 960
cgcttgctga tgtttatact gttttaagac ctgagttcgc gatccacggc gtaaacccaa 1020
tgcctgggcc gctcacacgt gctattgcac gcgccgccta tgtgtagtcc actttgcacg 1080
ggtgtgggtt acatatgcgg tgtgtcggtt gtgggatata tgtgacccat tcaaacgtct 1140
cttagattac acttac 1156
<210> 70
<211> 1125
<212> DNA
<213> 人工序列
<220>
<223> BTV1的NS2 DNA
<400> 70
gttaaaaaat ccttgagtca tggagcaaaa gcaacgtaga tttactaaaa acatttttgt 60
tttggatgca aatggcaaaa cattatgcgg agcgatcgca aagttgagtt cgcaaccgta 120
ttgtcaaatt aaaattggaa gagtaatagc ttttaaacct gtcaaaaatc cggaacctaa 180
gggatacgtg ctgaatgttc caggacctgg tgcgtacaga attcaggatg ggcaggatat 240
catcagcctg atgttgacac cacatggggt tgaagcgaca acggaaaggt gggaagagtg 300
gaagtttgag ggtgtcagtg taacgccaat ggctactagg gtacaacata atggtgtaat 360
ggttgatgct gagattaagt attgtaaagg aatgggaata gtgcaaccat atatgcggaa 420
tgattttgat cggaacgaga tgcccgattt accaggtgtg atgaggtcaa actacgatgt 480
tcgtgaactg cggcaaaaga tcaaaaatga acgagaatca gcgccacggc ttcaagttca 540
aagcgtggcg ccaagggaag agtcacgctg gatggatgat gatgaagcaa aggtggacga 600
agaggctaaa gagatgattc cgggaaccag cagattggag aagctgcgtg aagcgagaag 660
caatgttttc aaggaggtgg aagctggaat caactggaat ctggatgaga aagatgaaga 720
ggatagagat gagcgggagg atgaggagca ggtgaagact ctgagtgacg atgatgaaca 780
aggtgaggat gcgagtgacg atgaacaccc aaaaactcat ataactaagg aatacattga 840
gaaggttgca aagcagatta aattaaagga tgaacggttc atgagtctat caagcgctat 900
gcctcaggcg agcggtggat ttgatcgtat gattgtcacg aagaagctta aatggcagaa 960
tgtgccatta tactgttttg atgagtcatc gaaaaggtat gagttacagt gtgtgggtgc 1020
gtgtgagcgt gttgcttttg tctctaagga catgagcttg atcatattgc cggtcggcgt 1080
ttaggcgctt gtgaccgcgt ggttaggggg ggattttaca cttac 1125
<210> 71
<211> 1049
<212> DNA
<213> 人工序列
<220>
<223> BTV1的VP6/NS4 DNA
<400> 71
gttaaaaaat cgcatatgtc agctgcgatg cttcttgcac ccggtgacgt gatcaagcgt 60
tcatccgagg agttaaaaca gagacagatc caaattaatt taattgattg gacagagggc 120
gagagcgaaa aagagagtaa agcggaagcc aaagagggag acaaagcaga ggaattaaaa 180
gatggtgagg ggacgcaatc ggagagcagc cagaagaaag agagcagcaa agagactaaa 240
gatgcagatg tggatagacg catacatact gcagtgggat ctggatcaag cgcaaaagga 300
cctggagaac gcgcgaacga gaatgttgac agaggagatg gaaaggttgg aggaggaggt 360
ggagatgctg atgcgggagt tggaactact ggagcgaatg gaggaagatg ggtagttcta 420
acagaagaga ttgctcgcgc gatcgaatct aaatacggta cgaagattga tgtttacaga 480
gatgaggtcc cagcacaaat catcgaagta gaacgcagtc tccagaaaga gctagggatt 540
tcgcgtgagg gagtggccga gcagacagag agattgagag atctgcgacg aaaagagaag 600
agtggggcac atgcaaaggc tgctgagaga gggaggcgta aacaagggaa gaagcctcat 660
ggtgatgcgc agagagaggg taccgaggaa gagaagacgt ccgaagaacc agcgagtgtc 720
gggatcacta tagagggggt catgagtcag aagaaactct tgagcatgat tggcggtgta 780
gagagaaaga tggctccaat aggcgcgcgg gagagcgcag ttatgttagt ttcaaacagt 840
attaaagatg tggtgcgagc tacagcgtat ttcacagcgc caaccggaga tccacattgg 900
aaggaggtgg ctcgcgaagc ttcaaaaaag aagaacatat tagcatacac gagtacggga 960
ggagatgtga aaacggagtt cctgcatttg atcgatcacc tctaaagggt ccagggtacc 1020
ttcttgacgt agggcgattt tacacttac 1049
<210> 72
<211> 822
<212> DNA
<213> 人工序列
<220>
<223> BTV1的NS3 DNA
<400> 72
gttaaaaagt gtcgctgcca tgctatccgg gctgatccaa aggttcgagg aagaaaaaat 60
gaaacataat caggaacggg ttgaagagct gagtctggtc cgtgtggatg acacgatttc 120
ccaaccgccg agatatgctc cgagtgcgcc tatgccatcg tcgatgccta ctgttgcact 180
tgaaatcttg gacaaagcaa tgtcaaatac aacgggtgct acgcaaacac aaaaagcgga 240
gaaggctgca ttcgcatcgt acgcagaagc gtttcgtgat gacgtgaggt tgagacagat 300
taaacgacat gtaaatgaac aaattctgcc aaagttgaaa agtgacctag gaggcttaaa 360
gaagaaaaga gctataatac atatgacatt actgatagcg gcggttgttg ctctgttgac 420
atcagtttgc acgttatcga gtgatatgag cgtggcgttt aagctaaatg gtacatcagc 480
tgagatacct caatggttta agagtctgaa cccaatgtta ggtgtggtga atttgggggc 540
taccttcctg atgatggtct gcgcaaaaag cgagagaagc ttgaaccagc aaattgacat 600
gattaagaag gaagtgatga agaaacaatc atataatgat gcggtgagga tgagctttac 660
ggaattttca tcagtcccgc tagatggttt cgaattacca ttaacctaag atcagtaggt 720
agagtggcgc cccgaggtct gcatcgtgta gagtggttga tctcacgatg cagactccta 780
ctgctgtcta acgggggagg gtatgcggcg ctacacactt ac 822
<210> 73
<211> 2939
<212> DNA
<213> 人工序列
<220>
<223> BTV8的VP2 DNA
<400> 73
gttaaaatag cgtcgcgatg gaggagctag cgattccgat ttatacgaat gtattcccag 60
cggagctttt agatggatat gattacatca ttgatgttag tagtcgtgtt gaagaggaag 120
gtgatgagcc cgttaagcga cacgatgtaa cagagattcc taggaattca atgttcgata 180
ttaaggatga acatatacgt gatgcgatta tatacaaacc agtcaacaat gatgggtacg 240
tactaccgag agtattagat atcacgctga aagcctttga cgaccggaaa agagttgttt 300
tgaatgatgg tcatagcgaa tttcatacga aaacgaattg ggttcagtgg atgattgatg 360
acgcaatgga tgtccaacct cttaaggtag atatcgctca cacgcgctcg aggatcagcc 420
acgcactctt taactgtacg gtgcgattgc attcaaaaaa ggctgacacc gcatcttatc 480
acgttgaacc agttgaaatt gaatcgtggg gatgtaacca cacatggttg agtaggattc 540
atcatctagt gaatgttgaa ttatttcatt gttcacaaga ggcggcgtat acattgaaac 600
ccacatataa gataatatca aatgcggaac gtgcatcaac gagtgattcc tttaatggaa 660
ctatgatcga attaggtcgt aatcatcaaa ttcagatggg tgaccaggga taccagaagt 720
tgaaagaagg gttggtacaa gttcgaattg aagggaagac gcctttggta atacaagagg 780
aaataaccgc tttgaataaa ataagagaac aatggatcgc tcgaaatttc gatcaaaggg 840
aaatcaaggt tttagatctg tgtaggttgt tgtctacgat aggtaggaag atgtgcaata 900
ctgaagagga acctaagaat gaagctgatc tttcagtgaa gttccaaatg gagcttgacg 960
aaatatttcg accgggaaat aacgagcgta ccaacatcat ggggggcgga gtacatcgga 1020
aaaacgagga cagattttac gtactaatta tgatcgctgc atccgatacc aataaaggtc 1080
gtatatggtg gagcaatccg tatccctgcc tgcgtggagc gttaatcgcc gctgaagtcc 1140
aattgggtga tgtttacaat cttttaagga attggttcca atggagcgta cgacctacct 1200
atgtacctta cgataggaat agagaaagtg ataagtatat ttatagtaga atcaatttat 1260
ttgattcaac cctgagacca ggcgacaaaa tagtacattg ggagtataag ttgcttaatg 1320
aagtacgaga ggttagcatc aacaaaggta atgagtgcga tctgttccct gaggatgaag 1380
aatttaccac caagtttcat gaggcgcggt atacggagat gaagaaccaa attattcaaa 1440
gcgggtggaa tcaacgagat tttaaaatgc ataaaatact agaggatggc gcaaatgtgt 1500
taacgattga ttttgagaag gacgcgcaca tcggcacggg ttcggccctg agtttgccgg 1560
attactataa caaatggata attgctccga tgtttaacgc taagttacgc attaccgagg 1620
ttgtgattgg aacggctcac accgacgatc cagctgtagg gcgcagcgcg aaggcgttca 1680
cacatgatcc ttttgacctg cagcgatatt gtctagcaag atactatgat gtacgacctg 1740
gtatgatggg ccgcgctctc tctaagcaac aaaatatgtc gtcgatgact gataaactct 1800
ctaagcagga ggattacgca gggattgttt caaggcgtct ggaatacaag gaacgagaga 1860
acagatgctt gacggagaca gcgcagtatg tattcgagaa aacatgctta tacgtactag 1920
aattattgag cagacatacg atgccctcgg aggattctga agtaacgttt gaacacccaa 1980
caattgatcc cagcgtcgat attgaaacgt ggaaaataat tgatgtttcg caactgatca 2040
tattcgtatt tgactattta tttgaaaatc ggaagatcgt gagggatact accgaggcaa 2100
ggtggacgct tttcaaaata agatcggaag taggacgagc caggattgac gcaatagaga 2160
tgacttttcc tcggtttgga cgtatgctaa gaaatgcgtc tcaggcaaag ataaatcaag 2220
atatcgcgtg cttgaacttc cttccactac ttttcatcat aggcgataat atatcttacg 2280
cacatagaca gtggagtata ccggtgctgc tgtatgcgca cgatatccga attatacctt 2340
tggaagttgg agcgtacaat aatcgttttg ggctgacgtc atacctagag tatatggctt 2400
tctttccatc gtacgctact agagtagcta aaattgacga gtcgatcaag gagtgcgcga 2460
tcgcaatggc tgagttttat atgaacaccg atatccacag tgggagtgtg atgagtaacg 2520
tgataacgac caagcggcta ttgtatgaaa catacttagc ttcgctatgc ggcggttatt 2580
cggatggatt gctttggtac ctgccaatta cacatccctc gaaatgctta gtcgcctttg 2640
aggtagctga tgacgtagtt ccactctcag taaggcggga acgtattcta tctaggtttc 2700
ctttaagctc gagacatgtc aaggggattg cgttgatttc ggtggatagg aatcaaaagg 2760
tatcggttca aacagagggt attgtaacac atcgtctctg taaaaagaat ctgttaaaat 2820
acgtgtgtga tgttattctt tttaagtttt ctggacacgt ttttgggaat gatgagatgt 2880
taactaagct gctcaatgta tagctctcgt gactgagagc tcgcgcgcta tcaacttac 2939
<210> 74
<211> 1637
<212> DNA
<213> 人工序列
<220>
<223> BTV8的VP5 DNA
<400> 74
gttaaaaaag cgatcgctct cgcgaagatg gggaaaatca taaagtccct aagccgattc 60
ggaaagaaag ttggaaacgc cttaacatca aacacagcaa aaaagattta tagcacaatt 120
ggaaaagcgg ctgaacgatt tgcagaaagt gaaatcggct cagcggctat tgatgggtta 180
gttcagggga gtgtacattc gttgatgacg ggagagtctt acggcgagtc ggtaaaacaa 240
gctgtgctat taaatgtaat gggaagtggt gaagagcttc cagatccact aagtccgggt 300
gaacgtggaa tgcagacaaa gatccgtgaa ttggaggatg aacagcgtaa tgagttgatt 360
cggttgaagt ataatgataa gataaagcaa aaatttggga aagaattaga agaggtatat 420
gagtttatga atggggttgc gaagcaggag gaagacgaag agaaacatta tgatgttctc 480
aaaaaagcgg taaactcgta cgataaaatc ttaaccgagg aggaaaaaca aatgaggatt 540
ttagcaacag cgttacaaaa ggaggtgaag gagcggacgg ggacggaggc cgtcatggta 600
aaagagtatc gaaataagat cgatgcgtta aaagaggcca tagaggtaga gcgtgatgga 660
atgcaggaag aggcgattca ggagatagct ggaatgacgg cggacgtatt ggaggcggct 720
tcggaggaag tgccactaat aggtgcgggg atggcgacag ctgtagctac aggtagggcc 780
atcgaaggag cttacaaatt aaagaaggtt ataaacgctc tcagcggaat cgatttgact 840
cacttaagaa caccgaagat tgagcctacg atagtatcga ctgtgttgga tcataagttt 900
aaggatattc ctgatgagat gctcgcggta agcgttttat caaagaatag agccatcgaa 960
gagaaccata aagagattat acatcttaag aacgagatat tgcctagatt taagaaggcg 1020
atggacgagg agaaggagat atgtggaatc gaagacaaaa agatacatcc gaaagtcatg 1080
atgaaattca aaattccgcg aactcaacag ccccaaatcc atatatatag tgcaccgtgg 1140
gattccgacg acgttttctt ttttcattgt atatcgcatc accacgcgaa tgaatcattt 1200
tttattggat ttgacttagg catcgatctg gttcactacg aagatttgac tgctcattgg 1260
cacgctttgg gtgcggcgca ggcggcggtt gggaggagtc tgaatgaagt gtacaaagaa 1320
tttttgaacc tagcgattaa taacacgtat agctcgcaaa tgcacgctcg gaggatgata 1380
cgatcgaaga ctgtacaccc catatatcta ggctcactcc attatgatat ttcgttttcg 1440
acattacgga gcaatgcgca gagaattgtg tatgatgagg aattacagat gcacatattg 1500
aggggaccac tacatttcca aaggcgggcg atactaggcg cgataaaaca tggagttaaa 1560
atcttaggga cggaggttga tatcccactc ttcttaagaa atgcctgagc gcagcggagc 1620
caccgctttc cacttac 1637
<210> 75
<211> 2940
<212> DNA
<213> 人工序列
<220>
<223> 用于sBTV FJ969720的BTV1的VP2 DNA
<400> 75
gttaaaatag tagcgcgatg gatgaactag gcatcccagt ttataagaga ggatttcccg 60
aacacctgct tcgtggttac gagttcataa tagatgttgg aaccaagata gaaagtgttg 120
gaggacgtca tgatgtaacg aaaataccag aaatgaatgc atatgacatc aagcaggaga 180
gcatccgaac cgcattatgg tataatccga taagaaatga tggttttgta ttgccgcgag 240
tgctggatat cacattgagg ggttacgatg aaagacgggc ggttgttgaa agtacgagac 300
acaagagttt ccatacgaat gaccagtggg tgcagtggat gatgaaagat tcgatggacg 360
ctcagccttt aaaggttggg ttagatgatc aaagtaggaa tgtagctcac tcgttacata 420
actgcgtagt caaaatcgat tcaaagaagg ctgatactat gtcttaccat gtagagccga 480
tagaggacgc gtcaaaggga tgtttgcata cgagaaccat gatgtggaac cacctggtac 540
gaatagaaac atttcatgcg gcgcaggagg tggcatatac tcttaaacct acttatgaca 600
tcgtggtcca cgctgaaagg agagatcgta gtcaaccgtt taggccaggg gatcagacat 660
taattaattt tgggagaggt cagaaggtgg caatgaacca caattcatat gataagatgg 720
ttgagggatt aacacattta gtgattagag ggaaaactcc tgaggtgatt agagatgata 780
ttgcgagctt ggatgagata tgtaataggt ggatacagag taggcacgac cccggagaga 840
taaaagcata tgaattatgt aaaatattat caacgattgg tcggaaagtc ctcgatcgag 900
agaaagaacc agaggacgag gcgaatctat cgatccgatt tcaagaggca atcgacaata 960
agttccgaca acatgatcct gagcgcctga agatatttga gcataggaat caacgtagag 1020
atgaggaccg attctatatt ctgttgatga ttgcagcctc cgatactttt aatacacgag 1080
tgtggtggtc gaacccatat ccatgtttaa gaggaacctt aatcgcgtca gaaacgaagc 1140
taggtgacgt ttattcgatg atgcgttcat ggtatgattg gagtgttcga ccaacctata 1200
cgccttacga gaaaacgaga gaacaggaaa aatatattta tggacgggtt aacctgtttg 1260
actttgtcgc ggaacctggg attaaaattg ttcattggga atataggctg aatcattcca 1320
cccgggagat aacctatgcg caagggaacc catgtgattt atacccagag gatgatgatg 1380
taatagtcac aaagttcgat gatgtcgcgt atggccaaat gatcaatgag atgataaatg 1440
ggggttggaa tcaagaacag ttcaagatgc ataaaatttt aaaatcggaa ggtaatgttc 1500
taacgataga ttttgaaaaa gatgcaaagc taacaaccaa tgaaggcgta acgatgccag 1560
aatatttcaa taagtggata atcgctccga tgttcaacgc taagctgcgt ataaaacatg 1620
aagagattgc gcagcgtcaa agtgatgatc cgatggtaaa acgtacttta tcacctatta 1680
ccgcagatcc aatcgaattg caaaggttga ctttggcgcg gttttacgac attcgtcccg 1740
ctttaagagg acaggcgctt tcgcggcaac aggcacagtc cacttacgat gaagaaatat 1800
cgaaacagcg ggattatgcg gaaatattga aacgtcgtgg aattgtgcaa atcccaaaga 1860
aaccttgccc aactgtgaca gctcagtata cgctggagcg ttatgctttg ttcattataa 1920
acatcttaca acagcatgta gcacgagact gcgacgagga ggcgatatac gagcatccga 1980
aagcagatta tgaacttgaa atatttggcg agagcattgt ggatatctct caagtgatcg 2040
ttctagtttt tgacttgata tttgagagaa gaaggagggt aagagatgtg tatgaatcgc 2100
ggtacataat tgcgcgcatt aggaggatgc gaggtaaaga gagattgaac gtgatcgcgg 2160
agtttttccc aacctatggg agtcttctaa atggattaaa cagcgctacc gtagtacagg 2220
atattatgta tttgaacttt ctcccattgt attttttagc aggcgataac atgatatact 2280
ctcataggca gtggtctatt cctttacttc tatacactca tgaagtgatg gtggtcccat 2340
tagaagttgg ttcatacaat gatcggtgcg gattaattgc gtacctggaa tacatggttt 2400
tctttccctc caaggcgatt cgacttagca aactaaatga agcgcagccc aagattgcgc 2460
gcgagatgct taagtactac gctaatactg cggtatatga tgggggagtc aactacaacg 2520
ttgtgacgac gaagcagctt ctatatgaga cataccttgc ttcgttatgt gggggtattt 2580
ctgacggtat tgtctggtat ttgccgatca cacatccgaa caaatgcatc gtagcgatcg 2640
aggtatctga tgaaagggtt ccggctagca ttagagcggg gcgtataagg ctaagatttc 2700
cgctaagcgc gcgacatcta aagggggttg taatcataca aattgacgag gagggcgaat 2760
ttacagtgta tagcgaggga attgtgtctc atcgggtgtg taaaaagaat ttactcaagt 2820
atatgtgcga tattatatta ctgaagtttt cggggcacgt ttttggtaac gacgagatgc 2880
tgacaaaact tctcaacgta tgatctgctt gaccgcagat ccgcgcacta ttagacttac 2940
<210> 76
<211> 1635
<212> DNA
<213> 人工序列
<220>
<223> BTV8 sBTV FJ969723的VP5 DNA
<400> 76
gttaaaaagt gcgcccttag cgaagatggg taaagtcata cggtccttaa accgatttgg 60
caaaaaggta ggcaacgcgt taacttctaa taccgcaaaa aagatctata gtacaattgg 120
aaaagcggcg gagcgatttg ctgagagtga gataggttca gcggcgatcg atggattagt 180
acagggaagc gtacattcaa tcataacggg cgaatcttac ggcgaatctg tgaaacaagc 240
tgtgttgtta aatgtgttgg ggagtggtga ggaaattcct gatccgctaa gtccaggaga 300
gcgagggata caagctaagt tgaaagagtt agaggacgag caacgcaatg aattagttcg 360
cttaaaatat aatgataaga ttaaggaaaa atttggaaaa gagcttgaag aggtttacaa 420
ttttatgaat ggagaggcga atgctgagat tgaagatgag aagcagtttg atatactgaa 480
caaggcggtg acctcgtata acaaaattct tacggaggaa gatctacaga tgcgccggtt 540
agctacggcg ctgcagaaag agatcggaga aagaacacat gcggagacgg tcatggtgaa 600
agaataccgc gataaaattg acgctttaaa aaatgcgatt gaggtagaaa gagatggcat 660
gcaagaggag gcgatacagg agatcgcggg gatgaccgca gatgtgttag aggcggcatc 720
ggaggaggtt ccgctgattg gtgcggggat ggccacagct gtggcgacag ggagggctat 780
tgagggagcg tacaaactca aaaaagtgat caatgcttta agcgggatcg atttaacgca 840
tttacgcact ccgaaaatcg aacctagtgt tgtttcaact attcttgagt accgcgcaaa 900
ggagattcct gataacgctc tggctgttag tgttctatca aagaatcgtg cgattcaaga 960
aaaccacaaa gaactgatgc atattaagaa tgagatatta ccgagattta agaaagcgat 1020
ggatgaagaa aaggaaatat gcgggataga agacaaagtg atccacccga aggtcatgat 1080
gaagtttaag attccaagag ctcaacagcc gcagattcat gtatacagtg ccccatggga 1140
ttctgatgat gtgtttttct ttcattgcat ctcacaccat catgcaaatg agtcgttctt 1200
cttaggtttc gatttgagca ttgacttagt tcattatgaa gatctcaccg cccattggca 1260
tgcattggga gcagctcaaa cggcagcggg acgtacgcta actgaagcat atagagagtt 1320
tctaaatttg gcgatttcaa atgcatttgg cacgcaaatg cacacgagaa gattggttag 1380
gtcgaaaacg gtacatccga tttacttagg ttccttgcac tacgatattt ccttttcgga 1440
tctgcgtgga aacgctcaga gaattgttta tgacgatgaa ctgcagatgc acatacttcg 1500
tgggccgata cattttcaga gacgggcaat actaggggct ttgaaatttg gatgtaaggt 1560
tttgggggac cgtttagacg taccactctt cttacgaaat gcttgaacgc agcgacggga 1620
agcacttaca cttac 1635
<210> 77
<211> 2943
<212> DNA
<213> 人工序列
<220>
<223> BTV2 JN255863的VP2 DNA
<400> 77
gttaaaacag gatcgcgatg gatgaacttg gcattccgat ttatggacgt aactacccag 60
agcatttatt aaaaggatat gagtttttga ttaataccgg cgtgagatat ccaagtcaag 120
gtggacgtca tgatgtttcc aagattcctg agatgtttgc ttacgatatc aaagatgagg 180
ggatacggga agcactcaaa tatatgccaa cacgaaatga tggggtggtc ctgccaagga 240
tcgttgacat tagcttgaag ggatatgata tacggaaatc agttattgaa gcgaataaga 300
gtaacagctt ccatactgac acaaactggg ttcagtggat gattaaagat agtatggatc 360
aacagccgct taagatatcg attgacgagg aacatagccg ggttgtacac tccctattta 420
attgtcaagt gaagattgat gcgaaaaaag cagatacctt atcttaccat ctcgaagcaa 480
ttgaagatgc agagaaagca tgtttgcaca cgaggggtca attatgtaat catctggcga 540
ggatggatct gttacacgcg gcacaagaaa ttgcgtatgc aattaagccg acgtatcagt 600
taatagtgca ttcagagaga gcgtcaactt cagataactt cgaattaggg aggcaggatg 660
taattacttt gagaagggga catagaatac aaatgggtga cgaagcgtat actaagctga 720
tggaacgttt agtgaggtta acggtgcaag ggaatgtgcc gagaaagata caaagtgaga 780
tagagcagct tgaggcgatt cgaactaggt gggcaactgg cagatatgat ccagctcata 840
tcaattcaca agatctgtgt cggatattgt cacgcatagg cagaattatg ttggaccaag 900
aagcggaacc ggttgatgaa gatagcttat ctttgcggtt tcagcgagct ctcgatgaga 960
agttccgtct taacgattct gagagaaata agatatttga gcccaaatcg cataggaagg 1020
atgaggatag gttctacgtt ttgttagcta tcgctgcttc cgacacttac aattcgagaa 1080
tttggtggtc aaatccgtat ccatgcttgc gcggcacgtt gattgcggcg gagacaaaat 1140
taggcgatgt ttactttact ctacgatctt ggtatgactg gagtgttaga agtagctatg 1200
taccgcgtga aagagagcgg gaaacggaga agtatatatt tagtaagata aatctatttg 1260
attacgaagc tggtccatcg agcaaggtca tacattggga atatcagtta tataaacgag 1320
agcgggtggt taccctagag agaggcaatc cttgtgacct gtatcctgat gaagatgatg 1380
aagttattat cacgaaattt gatgacgcca aatactctga gatggtcggg gaaattatag 1440
atggtggatg gaatgatgag gaatttaaaa tgtataagtt acttcaggaa aaaggcaatg 1500
ttctcacaat tgacttcgaa aaggatacga agctgaatag tacttcagag gtggttttac 1560
cggattatta tggtaagtgg atagttgcgc cgatgttcaa ctctaaaatg agaattattg 1620
aaacggagat cgcaaccaac agaagtgatg atcccatgat taaaaggacg ctcaaaccga 1680
tgactgatga tcccgttgag ctacagagat atactttggc cagatactat gatattcgtc 1740
ccggactaat gggtcgttcg ctaaatagga cacagactca atcaacattt gacgcgaagg 1800
tgtcagaatt gtccgactat gaaaaggtgg tgtcacggtt tggtgtaatc aagaaaccga 1860
caagaccttg cgtaactttg actggacgat acatcctgga gaaatattct ttacttctta 1920
tagatatatt gaagtatcat actgaggttg aagagaatcc acaagaagaa tttactcatc 1980
cgaggattga ttcacagttt aaattcaacg gtaatacatt atctgatctg aatcaaacgg 2040
tcgttttcat agtggattat ttgcatgaga agcgtaacta tgtacgatcg gtatacgagg 2100
cgagatacat tatatctagg ataaggtcgt ctaccggtgc ggcgcgtatg agtatcctcg 2160
aattttattt cccaacattc gcacgcttga tttcgaatgc gcgggaaccg acttatgtga 2220
aagatctgat ggcgttgaac ttcttgccgt tactattcat agtaggtgat aatatgatat 2280
ataaacatag gcagtggtcg ataccattat tactgtatac agaccgagtg aaggtgattc 2340
cattggaagt gggctcatcg aacaatagac agggactcgt atcctatcta gagtatatgt 2400
tcttttttcc atcgttagct gatcgaacat ctaaggtgga tgaatcgatg attaaggtgt 2460
caaaggaggt ggttaattac tatatgaaaa caacaatatc ggaagggggc gtgaatttga 2520
atgttgtttc caccaagtca ttgctatatg acatctatct ctcatcagtg tgcggtggtg 2580
tgtcggatgg tgtagtctgg tacctaccga tcactcatcc atataaatgt gtagtcgcta 2640
ttgaggtgtg cgacgataga gtgcccgcgc gcttacgttg tgatagattg aggttgcgtt 2700
tccccttaag tgcacagcat ttaaagggta tagtagtgat tcaaataaat gaggagggcg 2760
gatttgatgt gtatacagag gggatagtaa cgcatagggt ttgtaagaaa agtttactta 2820
aacatgtatg cgacattgtt ctactaaaat ttcatgggca cgtctttggg aacgatgaaa 2880
tgctaacaaa gttactcaat gtatgacctg cgtgactgca ggtccgcgat ctgttcaact 2940
tac 2943
<210> 78
<211> 1635
<212> DNA
<213> 人工序列
<220>
<223> BTV2 JN255867的VP5 DNA
<400> 78
gttaaaaagt gtacccttag cgaagatggg taaagtcata cgatctctga gcagatttgg 60
aaagaaagta ggtagcgcgc tcacgtctaa cacggcaaaa aagatctata gtacgatagg 120
caaagcagcc gagagatttg cggagagcga gatcggttca gcagcgatcg atggtttagt 180
acagggcagt gtacactcga tattgacagg tgagtcttac ggtgaatcag tcaaacaggc 240
ggtcctacta aatgttctgg gtagcggcga agagattcca gatccgctaa gcccggggga 300
acaaggtata cagactaaat tgaaagaatt agaggaggaa cagagaaatg agctggtacg 360
attaaaatat aatgataaaa ttaaagaaaa gtttggagag gagctggagc aggtatatga 420
atttatgagt ggtgcggcaa aagaagaggc gggggatgag aagcagttcg atatcttaag 480
taaggcggtg aattcgtaca ataagatatt aactgaagag gacctacaaa tgcgtcggct 540
cgcgatcgct ttacagaagg agattggtga gaggacacac gcggagacaa ctatggtaaa 600
agagtatcgg aataagattg acgctcttaa gagcgctata gaaattgaaa gagacggcat 660
gcaggaagaa gcgatacaag aaatagcggg gatgactgct gatgtcttag aggcagcatc 720
tgaagaggtg cctctgatcg gagcgggcat ggcaacggct gttgcgacgg gaagagcgat 780
tgagggagcg tataagttga agaaggtaat taacgcatta agcgggatag atctaacaca 840
tcttcgtacg ccgaaaatcg agcctagcgt agtctctaca attttggagt atcgcgcgaa 900
ggatatcccg gataatgcgc ttgcagttag cgtgctgtcg aaaaatcgcg cgattcaaga 960
aaatcataag gagttaatgc atatcaaaga tgagatctta ccccgtttta agaaagcgat 1020
ggatgaagag aaggagatct gtggaattga ggataaagta atacatccta aagtaatgat 1080
gaaatttaaa attccaagat cacagcaacc gcaaattcat atttatacgg cgccatggga 1140
ttctgatgat gtattctttt tccattgtat ttcacatcac cacgccaatg aatcattttt 1200
tatcgggttt gatttgagca ttgatttggt tcattatgaa gatttgacgg ctcattggca 1260
cgcactgggg gcggctcaga tggccgtagg acgcacgcta accgaagcgt ataaagaatt 1320
cttaaatttg gccatttcta gcacgtatgg gacgcaaatg cacactcgaa ggttaattag 1380
gtcgaaaacg gtgcatccga tttatttggg ttctctccat tacgatattt cttttgctga 1440
cttacgggga aacgctcaac gcatcgtata cgatgatgaa ttacaaatgc atatcttgcg 1500
tggtccgcta cattttcaaa gacgagctat actaggtgcg ctaaagtttg ggtgcaagat 1560
acttggagat cgattggacg tgcccctctt cttacgaaat gcttgaacgc agcgacgggc 1620
agcacttaca cttac 1635
<210> 79
<211> 2943
<212> DNA
<213> 人工序列
<220>
<223> BTV2 AJ585123的VP2 DNA
<400> 79
gttaaaacag gatcgcgatg gatgagcttg gcattccaat ttatggacgt aattacccag 60
agcatttatt aaaaggatat gagtttttga ttaatactgg tgtaaaatat ccaagtcaag 120
gtggacgtca tgatgtttcc aagattcctg agatgtttgc ttacgatatc aaagatgagg 180
ggatacggga cgcactcaaa tatatgccaa cacgaaatga tggggtggtc ttgccaagga 240
tcgttgacat tagcttgaag ggatatgaca tacggaaatc agttattgaa gcgaataaga 300
gtaacagctt ccatactgac acaaactggg ttcagtggat gattaaagat agtatggatc 360
aacagccgct taagatatcg attgatgagg aacatagccg ggttgtacat tccctattta 420
attgtcaggt gaagatcgat gcgaaaaaag cagatacctt atcttaccat cttgaagcaa 480
ttgaggacgc agagaaagca tgtttacata cgagaggtca attatgtaat catctgacga 540
ggatggatct gttacatgcg gcacaggaaa ttgcgtatgc aattaagccg acgtatcagt 600
taatagtgca ttcggagaga gcgtcgactt cagataactt cgaattaggg aggcaagatg 660
taatcacttt aagaagggga catagagtac aaatgggcga cgaagcgtat actaagctga 720
tggaacgctt agtgaggtta acggtgcaag gaaatgtacc cagaaagata caaagtgaga 780
tagagcagct tgaagcgatt cgaaccagat gggcaactgg cagatacgac ccagctcata 840
tcaattcaca ggatctgtgt cggatattgt cacgtatagg cagaattatg ttggatcaag 900
aagcggaacc ggttgatgaa gatagcttat ctttgcgatt tcagcgagct ctcgatgaga 960
agttccgtct taacgattct gagagaaata agatatttga gcacaaatcg cataggaagg 1020
atgaggatag gttctacgtt ttgttggcta tcgctgcttc cgacacttac aattcgagga 1080
tttggtggtc aaatccgtac ccatgcttgc gcggcacgtt gattgcggcg gagacaaaat 1140
taggcgatgt ttactttact ctacgatcct ggtatgattg gagtgttaga agtagctata 1200
taccgcgtga aagggagcgg gaaacggaga agtatatatt tagtaagata aatctatttg 1260
attacgaagc tggtccatcg agcaaggtta tacattggga gtatcaatta tataaacgag 1320
agcgggtggt taccctagag agaggcaatc cctgtgactt gtatcctgat gaagatgatg 1380
aagtcattat cacgaaattt gatgacgcta aatactctga gatggtcggg gaaattatag 1440
atggtggatg gaatgatgag gaatttaaaa tgtataagtt acttcaggaa aaaggtaatg 1500
ttctcacaat tgacttcgaa aaggatacga agctgaataa tacttcagag gtggttttac 1560
cagactatta tggtaagtgg atagttgcgc cgatgttcaa ctctaaaatg agaattattg 1620
aaacggagat tgcaaccaac aaaagtgatg atcccatgat taaaagaacg ctcaaaccga 1680
tgactgatga tcccgtcgag ctacagagat atactttagc cagatactat gatatccgtc 1740
ctggactaat gggtcgttcg ctaaatagga cacagactca atcaacattt gaccccaagg 1800
tgaaaaagtt gcccgactat aaaaaggtgg tgtcacggtt tggtgtaatc aagaaaccga 1860
caagaccttg cgtaacttta accgggcgat acattctgga gaaatattct ttacttctta 1920
tagatatatt gaagtatcat actgaggttg aagggaatcc acaagaagaa tttacccatc 1980
cgaggattga tccacagttt aaattcaacg gtaatacatt atctgatctg aatcaaacgg 2040
tcgtttttat agtggattat ttgcatgaaa agcgtaacta tgtacgatcg atatacgagg 2100
cgagatacat tatatctagg ataaggtcgt ctaccggtgc ggcgcgtatg agtatcatcg 2160
aattttattt cccaacattt gcgcgcttga tttcgaacgc gcgggaaccg acttatgtga 2220
aagatctgat ggcgttgaac ttcttgccgt tactattcat agtaggtgat aatatgatat 2280
ataaacatag gcagtggtcg ataccattat tgctgtatac agaccgagtg aaggtgattc 2340
cattggaggt gggctcatcg aacaatagac agggattcgt atcttatcta gagtatatgt 2400
tcttttttcc atcgttagct gatcgaacat ctaaggtgga tgaatcgatg attaaggtgt 2460
caaaagagat ggttaattac tatatgaaaa caacgatatc ggaaggggcc gtgaatttga 2520
acgttgtttc caccaagtca ttactatatg aaatctatct ctcatcagtg tgcggtggtg 2580
tgtcggatgg tgtagtctgg tatctaccga tcactcatcc atataagtgt gtagtcgcta 2640
tcgaggtgtg cgacgataga gtacccgcgc gtttacgttg tgatagattg aagttgcgtt 2700
ttcccttaag tgcacagcat ttaaagggta tagtagtaat tcaaataaat gaggagggcg 2760
gatttgatgt gtatacagaa gggatagtaa cgcatagggt ttgtaagaaa agtttactta 2820
aacatgtatg cgacattgtt ctgctaaaat ttcatgggca cgtctttggg aacgatgaaa 2880
tgctaacaaa gttactcaat gtatgacctg cgtgactgca ggtccgcgat ctgttcaact 2940
tac 2943
<210> 80
<211> 1635
<212> DNA
<213> 人工序列
<220>
<223> BTV2 JN255867的VP5 DNA
<400> 80
gttaaaaagt gtacccttag cgaagatggg taaagtcata cgatctctga gcagatttgg 60
aaagaaagta ggtagcgcgc tcacgtctaa cacggcaaaa aagatctata gtacgatagg 120
caaagcagcc gagagatttg cggagagcga gatcggttca gcagcgatcg atggtttagt 180
acagggcagt gtacactcga tattgacagg tgagtcttac ggtgaatcag tcaaacaggc 240
ggtcctacta aatgttctgg gtagcggcga agagattcca gatccgctaa gcccggggga 300
acaaggtata cagactaaat tgaaagaatt agaggaggaa cagagaaatg agctggtacg 360
attaaaatat aatgataaaa ttaaagaaaa gtttggagag gagctggagc aggtatatga 420
atttatgagt ggtgcggcaa aagaagaggc gggggatgag aagcagttcg atatcttaag 480
taaggcggtg aattcgtaca ataagatatt aactgaagag gacctacaaa tgcgtcggct 540
cgcgatcgct ttacagaagg agattggtga gaggacacac gcggagacaa ctatggtaaa 600
agagtatcgg aataagattg acgctcttaa gagcgctata gaaattgaaa gagacggcat 660
gcaggaagaa gcgatacaag aaatagcggg gatgactgct gatgtcttag aggcagcatc 720
tgaagaggtg cctctgatcg gagcgggcat ggcaacggct gttgcgacgg gaagagcgat 780
tgagggagcg tataagttga agaaggtaat taacgcatta agcgggatag atctaacaca 840
tcttcgtacg ccgaaaatcg agcctagcgt agtctctaca attttggagt atcgcgcgaa 900
ggatatcccg gataatgcgc ttgcagttag cgtgctgtcg aaaaatcgcg cgattcaaga 960
aaatcataag gagttaatgc atatcaaaga tgagatctta ccccgtttta agaaagcgat 1020
ggatgaagag aaggagatct gtggaattga ggataaagta atacatccta aagtaatgat 1080
gaaatttaaa attccaagat cacagcaacc gcaaattcat atttatacgg cgccatggga 1140
ttctgatgat gtattctttt tccattgtat ttcacatcac cacgccaatg aatcattttt 1200
tatcgggttt gatttgagca ttgatttggt tcattatgaa gatttgacgg ctcattggca 1260
cgcactgggg gcggctcaga tggccgtagg acgcacgcta accgaagcgt ataaagaatt 1320
cttaaatttg gccatttcta gcacgtatgg gacgcaaatg cacactcgaa ggttaattag 1380
gtcgaaaacg gtgcatccga tttatttggg ttctctccat tacgatattt cttttgctga 1440
cttacgggga aacgctcaac gcatcgtata cgatgatgaa ttacaaatgc atatcttgcg 1500
tggtccgcta cattttcaaa gacgagctat actaggtgcg ctaaagtttg ggtgcaagat 1560
acttggagat cgattggacg tgcccctctt cttacgaaat gcttgaacgc agcgacgggc 1620
agcacttaca cttac 1635
<210> 81
<211> 2935
<212> DNA
<213> 人工序列
<220>
<223> BTV3 AJ585124的VP2 DNA
<400> 81
gttaaaaacg ctgtcccgag aatggaggag ctggtaatac ctgtgatttc acgacaattc 60
gataagaaac tagtggggcg ttacgagtac gtgatagaat tagctgaacc agagcaggat 120
gagtggacga atcacgacgt tacacagata ccaggcaggc ggatgtttga tgtagcgcag 180
caaggcatca gggaggcaat catttataag ccgctggata atgatggtga ggttttacct 240
cgcatcttag atatgtcaat cgcgtgttat gacatgagga aaaccatgat gaagaaggaa 300
ggtgtcgatt ttgtttcgaa cacgagatgg cttgagtgga tgattcaaga ttcgatggat 360
gtacagccat tgcgtgtcca gatgaaggaa gatcatagta cggttcagta tgacatgttt 420
tcagcgaaag tgcacatcga ttctagaaaa gcggacacca catcgtacca cgcaatcgct 480
gttgagacga aagccgagcg taaatgttgc catgtgcgta cagaagtgtg gaatagtgta 540
gtccgcaacc accttttcaa taccgcgcag gaatcttgtt acacatttaa acaaacatat 600
gaactgattg ttaactctga acggttgtca acggaagagg aatttagagt gggcgctccg 660
cagtttcata caatacagag gaaccatcgt atgcagttag gagataacgc ttatgataaa 720
tttctgaagg gactggttca gctaagagta tcaggtacga ctccagctaa gatacgtgat 780
gaggtcgcag cgcttgatgt tatcagagat aattggatcc gtggaagttt tgaccgttca 840
catataaaat cattagaact atgtagatta ctttcgagta taggtagaaa gatggtaaat 900
atggaggaag agccaaagga tgaaaaggat ttatcggtca aattccaatt cagattagac 960
gaaaaattct ctcccaacga tccagagcga aacgtaatat ttacctctaa aacccaccga 1020
actaatgagg acagatttta tgtattattg gtaattgcgg cgtctgacac gaataacggt 1080
cgagtgtggt ggtccaaccc ttacccgtgt ttacgaggcg cgttaattgc ggcggagtgc 1140
aaattgggcg atgtgtatca caccttaaga tcgaagtatg aatggggtgt tcgaccaacc 1200
tacaagccaa aagatctgga acgcgagaga gagaagtacg tcgttggacg tgtcaatttg 1260
tttgacctcg aaggtgagcc ggcgacgaag gtcatacatt gggagtacga attaatttcg 1320
ccgacgtact cagtgagtaa ccacaaggga aatcattgtg atttatatcc ggacgatgtc 1380
gaaattacga ctaaatttaa tgaagataga tatcgtgaga tgatccagtc ggtaattgat 1440
gatgggtggg accaaaagaa tcttaaaatg tataagatcc ttgaggaaga aggtaatcct 1500
ttactatatg atcttgaaaa agatattaag ctggatagcc aatcgcaggt tgtgttccca 1560
tcatactata ataagtggac acatgccccg atgttcaatg ctcgggttaa accatgtgat 1620
atagagttgg cggagagaaa aaacgatgat ccattcgtaa aaagaacctt gaaaccgatc 1680
aaagcggatt gcgttgactt gttacgatac catatgtctc attactatga tttaagacca 1740
tctctcaagg gagtgagttt atcgaataag cagacgccct caggtatcca tcaggctctc 1800
gtacaagatg atttatattc aaggctattg aggcggcgcg atgtggattt agattatagt 1860
tcgccatgtc ctattataac gaactatttt ctgttggaga agtttcatat tctaatcttg 1920
accatcatgg aaaaacatta ctgggagtta gatgatagcg atgatgtata tgagttcccg 1980
aaaattgatg catcggcgtt cgaagtggat ggtacactgt acgatatctc gcagactata 2040
gttcacatgt atgacagatt ttttgagaag agacgggtgc tacgatcgat cgacgaaagt 2100
agatggatcc ttcatctgat cagaatatct cagggtaggg aaaggttgga agtgattgag 2160
aggttcttcc cgaattatgg gaaagcaatg cgtcaaaggg acttcaagaa agttagagat 2220
gtaatgttct tgaattttct acccttcttc ttcttgactg gtgataacat ctcgtatgaa 2280
cataggcagt ggtcgatacc aataatatta tacgctgaca aattacgaat tttaccaatt 2340
gaagttggtg cgcattacaa caggtttgga gtgacatgca tcttggaact actgaacttt 2400
tttccatcct atgaaaaacg agaggagaag cttgaagagg atattgtgtt gtgtgctgat 2460
gcgatcgtga atttttatct tcagacgacg atctcaaatg gaggtgtaca gacaagtatc 2520
gtgtcgacta aggcgctgct ttatgagatg tatttatctt ccatatgcgg cgggtactct 2580
gaaggtgttt tgtggtatct tccaataacg catcccgtta agtgcttagt cgctttagaa 2640
gtatctgatg cattagtcgg agcggatgta cgtattgata agattaggcg gcgattcccg 2700
ctatcagcta aacatttaaa aggtatagtt caaatatctg ttcacccgaa ccgtactttc 2760
agcgttacga cctgtgggat tgtgaagcat aaggtgtgta aaaagacttt gctgaagcat 2820
cgatgtgacg ttattctatt acagacccct ggttatgttt ttggaaatga tgaactatta 2880
acgaagcttc tgaacatata accctgtgac aggggttggg cacgttttca cttac 2935
<210> 82
<211> 1637
<212> DNA
<213> 人工序列
<220>
<223> BTV3 AJ586697的VP5 DNA
<400> 82
gttaaaaaga tccccatgat cgcgaaagat gggcaaaata atcaagtcgc taagccgttt 60
tggcaaaaaa gtaggcaacg ctttaacctc aaatacagcg aaaaagattt atagtacaat 120
cggaaaggcg gcggagcggt ttgctgagag tgagatcgga tcagccgcaa tcgatgggct 180
agttcaaggc agtgtgcact caattatgac gggagagtct tacggtgaat cggtcaaaca 240
ggcagtactg ttgaatgtac taggagcagg agatgagatt ccggacccgt tgagtccggg 300
agagcgcggc attcaaatga agattaagga gatagaggag gagcagagga atgagttagt 360
gagactgaag catgggaaag agattgcgaa aaagtttggc gatgagttgg aggaaatata 420
tcagtttatg aacggcgagg tgaaagagga agttcagcag gaggagcaat ataaggtgct 480
ctgtaaagcg gttaactcgt atgagaaatt acttctagcc gaaaatgacg agatgcgtac 540
gctagcgcgg gcactacaac gtgaagccac tgaaagaact gagaccgaat ccaagatggt 600
gaaagagtat aggcaaaaga tagatgcatt aaaagcggca atcgaagtgg agagagatgg 660
gatgcaagag gaggccatcc aagagatcgc gggtatgaca gctgacgtat tggaagcagc 720
ttctgaagaa gtgccattag tgggttcagg aatggcaacg gccatagcaa cgggacgagc 780
gattgaaggt gcatataaac tcaagaaagt cataaacgcg ttaagcggaa tagacctttc 840
gcacttgcga accccgaaga tagaaccaac gatggttgca accactttag aacatagatt 900
cagagatata ccagataaag aattagccgt gagtatcctg gcgaagaata acgcgatcgt 960
agcgaataca agagaggttc aacatattaa ggaagaaatt ctaccgaaat tcaagaaaat 1020
aatggatgag gagaaagaat tagaagggat cgatgataag aagatacatc cgagagtaat 1080
gatgaggttc aaggttccac gaccgcagca gcctcaaatt catatctaca gtgcaccatg 1140
ggactctgac gatgttttct tttttcattg tgtgtcgcat tttcatgtta atgagtcctt 1200
ctttctaggt tttgatctgg gaatcgacgt tgttcacttt gaggacttag ctgctcattg 1260
gcacgctttg ggagccgctc aagaggcgaa agggcgtact ctgaatgaag cgtataagga 1320
gtttttgaac ttatctatcg gtagcgctta tacgtcacca atgcatgcga ggcgtatgat 1380
tagatcaaag acagtccatc cgatctacct aggatccatg cattatgaca tcacttacga 1440
aacgttaaaa tcgaatgcgc aaaggattgt ttatgatgat gaattgcaga tgcacatatt 1500
gagaggaccc ctacatttcc agcgacgcgc gatattgggc gcactcaaat tcggagtgaa 1560
ggtgcttggc gacaagatag atgtgccact ctttctaaaa aacgcgtgac agcggtgatc 1620
ccgggactta cacttac 1637
<210> 83
<211> 2926
<212> DNA
<213> 人工序列
<220>
<223> BTV4 DQ191280的VP2 DNA
<400> 83
gttaaaagag tgtcccacaa tggaggagtt tgtcattcct gtgttttctg agagggagat 60
cccttacgct ttgattaatc gatatccttt ggcaatccag acggatgtta agattgcgga 120
cgtggaaggt tatcataatt tagcgaagat tccagaatcg gatatggttg atgtgcctaa 180
actagatatc gttagcgcgc ttaactataa accaacgaga aatgatggta tagtggttcc 240
gaggctgttg gatattaccc tgaaggctta cgatgataga aagtcggtta agaacgcacg 300
cggcgtggat tttatgacgg atgctaagtg gatgaagtgg gcgatcgatg ataggatgga 360
cattcagcca ctcaagatca ctctagatga acattacgca gtcaatcacc agctgtttaa 420
ttgcatagtg aaagcaaaaa ccgctaacgc tgacactata tattatgact attttcccct 480
cgaagataga gctaagaagt gcaaccatac gaatcttgaa ctattgagaa gtttaacaac 540
gattgaggcg ttccacgtac ttcaaggcgc tgcttatagt ctgaaatcaa actacgatct 600
aatcgcgaac tcagaaaggg aaagcttaga agaaagttat ccgataggtt cggaaaaatg 660
ggtacatctc ataagacgga ccaaaatagg aaacagcggt ctatcatata acagattcat 720
ctctagcatg gtgcaagtgg ttgttagggg gaaagtgcca gatataataa gaggagagat 780
tacacagctc aacaggataa ggacggagtg gataagcgcg tcatatgata gaactcggat 840
tagggcgttg gagttatgta atatattatc cgccattgga cgtaagatga tggacgcgca 900
tgaggaacct aaggatgaaa tggatttatc tactagattt caatttaaat tagatgagaa 960
gttcaataca tcggactctg aacatgtgaa catatttcgg acgagtgggg ctgccacaaa 1020
tgagggtaga ttctatgcgc ttatcgcgat agccgcgacg gatactcaga aaggccgagt 1080
ttggcgaaca aatccatacc catgtctgcg tggagcactg atagcatccg aatgtgagtt 1140
aggagacgta tattataccc tacgtcacgt gtatcggtgg agcttgcggc cggaatacgg 1200
tcaacgggaa cggcaattgg aggacaataa atacgtgttc ggtcgcgtga atttattcga 1260
ttcggattta gctgttggag atcaggttat tcactggcga tatgagataa ccgaaccagt 1320
taaaactact tatgacaacg gctacatatg taatccggag gaaaaagacg atgagctact 1380
gtgtaaaata gatgatgagc gatacaaaga gatgatggag aggttgatcg aaggtggatg 1440
ggatcaggag cggtttaaac tacataatat cctcacggag ccgaatctat tgacaatcga 1500
ttttgagaag gacgcgtacc taaactctcg gtcggagcta gtgttcccaa actactttga 1560
caaatggata aattctccca tgtttaacgc taggttgagg ataactcatg gagagattgg 1620
atcatctaaa acgatcgatc cgtggaatag gcgcgtagtt tatggatata ttaaaacatc 1680
tatagagtca ctcgattata cgcttgggaa gtactacgac atacggttac aactctttgg 1740
tgatactttg agtcagaaac aaacccaatc agccgtattt acgtacttaa gtggacagga 1800
tgacttccct gcattgacta attatttgaa gggtgaagca gtgtgcccac acgcgggtgg 1860
cgcagtatat acattccgta agatggcttt atctctgata gctagttatg agaagctgag 1920
tcctgaaatg cacgaaggct tagaacatca gacgtatgta catccttccg tgaacacagc 1980
ctaccagaag caggttaaag acatgaagga tctttctcag ttgatctgtt tcataatcga 2040
ttgtattttc gagaagcgtg tacaaatacg cggggttgga gaggcaaggc gcatcgttta 2100
cctaattcag aactcgacgg ggttgcaacg tcaagaagta ttgaagagaa cttttcctaa 2160
ttttctcatg cgtttgttca aactacgaga agtaaagcgc atctgtgact taaacgtgat 2220
caactttctg ccgttactat ttcttgttca agataatatc tcttattggc ataggcaatg 2280
gtcagtacct atgatattgt ttgacgacgc agtcaggttg ataccggttg aggttggagc 2340
ttacgcgaat agatttggat ttaaaagttt ttacaacttt attagattcc atcccggtga 2400
ttctaagaag aaacaagatg ccgacgacat gcataaagag tacggcgttg cctgcttcga 2460
atattacatg aacacgaaga tatcgcaggg tggagtgaat gtacctgttg tgacatccaa 2520
gttggacacg ctgaagatac atctagcttc tctatgctct gggcttgctg attcgatcgt 2580
ttacacgttg ccggtcgcgc atccgaagaa atgtatcgtt ctaatcgtcg taggagatga 2640
taagctcgac ccgcaggtac gctcggagca ggtattgagt aagtattatt attcacgcag 2700
acatatatgc ggagttgtag ctgtaagtgt gggtcaagag ggccagttac aggtgtattc 2760
caccggtata gtaagacatc gtatatgtga gaaatccata ttgaagtata agtgcaaagt 2820
tgttttggtt agaatgcctg gacatgtgtt cggaaacgac gaactgatga cgaaattact 2880
caacgtttag gtcctttgac acggacctgt ggcctcttac acttac 2926
<210> 84
<211> 1638
<212> DNA
<213> 人工序列
<220>
<223> BTV4 AJ586699的VP5 DNA
<400> 84
gttaaaaagt gttctcctac tcgcagaaga tggggaagat aattaaatcg ctaagtagat 60
ttggaaaaaa ggttggaaac gcattgtcgt caaacacagc gaagaaaatt tactcaacca 120
ttgggaaggc tgcggagcgg tttgctgaaa gcgagatcgg tgcggcgaca atagatggct 180
tagtgcaggg tagcgttcat tccataatta caggtgaatc ttatggagag tcagttaaac 240
aggcggttct cctcaacgtg ctaggtacag gtgaagaatt accagaccca ctgagccccg 300
gcgagcgtgg tatgcagaca aaaataaaag agttagaaga cgagcagcga aatgaacttg 360
ttcgattaaa gtataataaa gagataacaa aggagtttgg gaaggagcta gaagaagttt 420
acgacttcat gaatggtgag gcgaaggagg aggaagtagt tcaggaacaa tactcaatgc 480
tatgcaaagc agtagattca tatgagaaaa tattaaaagc agaggactcg aaaatggcaa 540
tattggcgcg cgcgttgcag cgggaggctt cagagagaag tcaagatgaa atcaaaatgg 600
tgaaagagta cagacagaag atcgatgcgc ttaaagatgc gatcgagatt gagcgagatg 660
gaatgcagga ggaggcgatc caggagattg ccggaatgac tgcggacgtc ttagaagcgg 720
catcggagga ggtgccccta attggcgcgg gtatggccac agctgtagcg accggtagag 780
caatagaagg cgcatataaa ttgaagaaag tcataaacgc attgagcggg attgatttat 840
cacatatgag gagcccgaag attgagccta ctatcatagc tacaacattg gagcatcgat 900
ttaaagagat accagatgag cagcttgcgg taagtgtgct gaataaaaag acagccgtga 960
ccgataactg caatgaaatt gcgcacatca aacaggaaat attaccaaag tttaagcaga 1020
ttatggatga agagaaggag attgaaggaa tagaagataa agtgattcat ccgcgggtga 1080
tgatgaggtt caaaattcct agaacgcagc aaccgcaaat tcacatttat gcggccccgt 1140
gggattctga cgatgtattt ttctttcatt gcgtttcgca ccatcatcgg aatgaatctt 1200
ttttcctggg attcgatcta ggaatcgatg tcgttcactt tgaagattta accagccatt 1260
ggcacgcgtt gggactagca caagaagcga gcgggcgtac gttaacggag gcgtatcgtg 1320
aatttctcaa tttatcaatc tcaaatacgt atagtagcgc tatacatgcg aggcgtatga 1380
tcaggtcgcg ggcagtacac cctatttttc taggatcgat gcattatgat attacgtatg 1440
aagctttaaa aaataatgcg cagagaatag tttatgatga ggaactgcaa atgcatatac 1500
taaggggacc tttgcacttc cagcgtcgag ctattttagg ggcgctgaaa tttggagtta 1560
aaatattagg cgataaaatt gatgtccccc tcttcttacg aaatgcttga acgcagcgag 1620
ggagaagctt acacttac 1638
<210> 85
<211> 2926
<212> DNA
<213> 人工序列
<220>
<223> BTV4 AJ585125的VP2 DNA
<400> 85
gttaaaagag tgtcccacaa tggaggagtt tgtcattcct gtgttttctg agagggagat 60
cccttacgct ctgattaatc gatacccttt ggcaatccag acggatgtta agattgtgga 120
cgtagatggt tatcataatc tagcgaaaat tccggaatcg gacatggttg atgttcccaa 180
actagacatc gttagcgcgc ttaactataa accaacgagg aatgatggta tagtggtacc 240
gaggctgttg gatatcaccc taaaggctta cgatgacaga aagtcggtta agaacgcacg 300
cggcgtggat tttatgacgg atgctaagtg gatgaagtgg gcgatcgatg ataggatgga 360
cattcagcca ctcaagatca ccctagatga acactacgca gtcaatcacc agctgtttaa 420
ttgtatagtg aaggcgaaaa ctgctaacgc tgataccata tattatgact attttcccct 480
cgaagataga gctaaaaagt gcaaccatac aaatcttgaa ctattgcgaa gtttgacaac 540
aattgaagcg ttccacgtac ttcaaggcgc tgcttatagt ctgaaatcaa actatgatct 600
aatcgcgaac tcggagaggg aaagcttaga ggaaagttat ccgataggtt cggagaaatg 660
ggtacacctc acaagacggg ccaaaatagg aaatagcggt ctatcatata acagattcat 720
ctctagtatg gtgcaagtgg ttgttagagg gaaagtgcca gatataataa ggggggaaat 780
tacgcagctt aacaggataa ggacagaatg gataaacgcg tcatatgata gaactcggat 840
tagggcgttg gagttgtgta atatattatc cgctattgga cgtaagatga tggacgcgca 900
tgaggaaccg aaggacgaaa tggatttatc tactagattt caatttaaat tggatgagaa 960
gttcaataca tcggattctg aacatgtgaa catatttcgg acgagtgggg ctgccacaaa 1020
tgagggtaga ttttatgcgc ttatcgcaat agctgcgacg gatactcaga aaggtcgagt 1080
ttggcgaaca aatccatacc catgtctgcg tggagcgctg atagcatccg aatgtgagtt 1140
aggagacgta tattatactc tgcgtcacgt gtatcgatgg agcctgcgac ctgaatacgg 1200
tcagcgggaa cgacaattag aagacaataa atatgtgttc ggtcgcgtga atttattcga 1260
ttcagattta gctgttggag atcaggttat tcactggcga tatgagataa ccgaaccagt 1320
caaaactact tatgataacg gctacatatg caatccagag gaaaaggacg atgagctact 1380
gtgtaagata gatgatgagc gctataagga aatgatggag aggttgatcg aaggtggatg 1440
ggatcaggag cggtttaaac tccataatat cctcacggag ccaaatctgc tgacaatcga 1500
ttttgagaaa gacgcgtacc taaattctcg gtcggaacta gtgttcccaa actactttga 1560
taaatggata aattctccca tgtttaacgc caggttgagg ataactcatg gagaaattgg 1620
gtcgtctaaa acggccgatc cgtggaatag gcgcgtagtt tatgggtata tcaaaacatc 1680
tatagagtca cttgattata cacttgggaa gtactacgac atacggttac agctctttgg 1740
tgatactttg agtcggaaac aaactcaatc agccgtattc acgtacttaa gcggacagga 1800
tgatttccct acattgacta attatttgaa gagtgaagca gtgtgcccac acgcgggggg 1860
cgcggtatat accttccgta agatggcttt atctctgata gctaattatg agaaactgaa 1920
tcctgaaatg cacgaaggct tagaacatca aacgtatgta catccttccg tgaacacaac 1980
ctaccaaaag caggttaaag atatgaagga tttctctcaa ttgatctgct ttataatcga 2040
ttgtattttc gagaagcgta tacaaatacg cggggtcgga gaagctaggc gcatcattta 2100
cctaattcag aactcgatag gattacaacg tcaagaagtg ttgaagagaa cctttcctaa 2160
ctttctcatg cgtttattca aactacgaga agtaaagcgc atctgtgact tgagtgtgat 2220
caactttctg ccgttactat ttcttgttca agataatatt tcttattggc ataggcagtg 2280
gtcggtcccc atgatattgt ttgacgacgc ggtcaggttg ataccggttg aggttggggc 2340
ttacgcgaat agatttggat ttaaaagctt ttacaacttt attagattcc atcccggtga 2400
ttctaaaaag aaacaagatg ccgatgacat gcataaagag tacggcgtcg cctgcttcga 2460
atattatatg aacacgaaga tatcgcaggg tggagtgaat gtacctgttg tgacgtccaa 2520
gttggacacg ttgaagatac atctagcttc tctatgctta gggctcgctg attcaatcgt 2580
ttacacgctg ccggttgcac atccgaaaaa atgtatcgtt ctaatcgtcg ttggagatga 2640
taagctcgac ccgcaggtac gctcggagca ggtattaagc aagtactatt attcacgcag 2700
acatatttgc ggagttgtag ctgtaagtgt aggtcaagag ggccagttac aggtgtattc 2760
ctccggtata gtaagacatc gtatatgtga gaaatctata ttgaagtata aatgcaaagt 2820
tgttttggtt agaatgcctg gacatgtgtt cggaaacgac gaactgatga cgaaattact 2880
caacgtttag gtcccttgac acggacctgt ggcctcttac acttac 2926
<210> 86
<211> 1638
<212> DNA
<213> 人工序列
<220>
<223> BTV4 AJ586699的VP5 DNA
<400> 86
gttaaaaagt gttctcctac tcgcagaaga tggggaagat aattaaatcg ctaagtagat 60
ttggaaaaaa ggttggaaac gcattgtcgt caaacacagc gaagaaaatt tactcaacca 120
ttgggaaggc tgcggagcgg tttgctgaaa gcgagatcgg tgcggcgaca atagatggct 180
tagtgcaggg tagcgttcat tccataatta caggtgaatc ttatggagag tcagttaaac 240
aggcggttct cctcaacgtg ctaggtacag gtgaagaatt accagaccca ctgagccccg 300
gcgagcgtgg tatgcagaca aaaataaaag agttagaaga cgagcagcga aatgaacttg 360
ttcgattaaa gtataataaa gagataacaa aggagtttgg gaaggagcta gaagaagttt 420
acgacttcat gaatggtgag gcgaaggagg aggaagtagt tcaggaacaa tactcaatgc 480
tatgcaaagc agtagattca tatgagaaaa tattaaaagc agaggactcg aaaatggcaa 540
tattggcgcg cgcgttgcag cgggaggctt cagagagaag tcaagatgaa atcaaaatgg 600
tgaaagagta cagacagaag atcgatgcgc ttaaagatgc gatcgagatt gagcgagatg 660
gaatgcagga ggaggcgatc caggagattg ccggaatgac tgcggacgtc ttagaagcgg 720
catcggagga ggtgccccta attggcgcgg gtatggccac agctgtagcg accggtagag 780
caatagaagg cgcatataaa ttgaagaaag tcataaacgc attgagcggg attgatttat 840
cacatatgag gagcccgaag attgagccta ctatcatagc tacaacattg gagcatcgat 900
ttaaagagat accagatgag cagcttgcgg taagtgtgct gaataaaaag acagccgtga 960
ccgataactg caatgaaatt gcgcacatca aacaggaaat attaccaaag tttaagcaga 1020
ttatggatga agagaaggag attgaaggaa tagaagataa agtgattcat ccgcgggtga 1080
tgatgaggtt caaaattcct agaacgcagc aaccgcaaat tcacatttat gcggccccgt 1140
gggattctga cgatgtattt ttctttcatt gcgtttcgca ccatcatcgg aatgaatctt 1200
ttttcctggg attcgatcta ggaatcgatg tcgttcactt tgaagattta accagccatt 1260
ggcacgcgtt gggactagca caagaagcga gcgggcgtac gttaacggag gcgtatcgtg 1320
aatttctcaa tttatcaatc tcaaatacgt atagtagcgc tatacatgcg aggcgtatga 1380
tcaggtcgcg ggcagtacac cctatttttc taggatcgat gcattatgat attacgtatg 1440
aagctttaaa aaataatgcg cagagaatag tttatgatga ggaactgcaa atgcatatac 1500
taaggggacc tttgcacttc cagcgtcgag ctattttagg ggcgctgaaa tttggagtta 1560
aaatattagg cgataaaatt gatgtccccc tcttcttacg aaatgcttga acgcagcgag 1620
ggagaagctt acacttac 1638
<210> 87
<211> 2921
<212> DNA
<213> 人工序列
<220>
<223> BTV5 AJ585126的VP2 DNA
<400> 87
gttaaaagct tctcaggatg gatgagttcg gtgttccgat atttcggtcg agccaagttc 60
cccaccacat actcgaagga tatgatatta aggtcgactt gagtgatagg gtggtgcaag 120
aggatggtcg tcatgatcca aataagttaa aagaacgaaa aacgatagat gtaccaaatg 180
gtgaagccag atctgatctg aattacaacc caacggataa tgaaggcaac cagctaccac 240
gtgcgctaga cattgcatta tgtgcgcggc ttacgaggaa aagtttcaac acaaacagtg 300
gtgttacgtt tcatacggat cacaaatgga tggaatggat gctgtccgat gcaatggatg 360
ttcaaccact gcgaatcgac ttagcagcgc agaacgagta cgcaaaatgt gacctattta 420
attcatcggt ctatgtcagg aaaaaatatg ttgatgcaat ctcatataga tatatatcag 480
tggaagatga ttctaagggg tgcaatcata cgagggttta tagcgtgaat catatgataa 540
attgtggatt gtataacgtg gcgcaagagt gtgcgtacgc gtttaagcat acgaatacgt 600
taatagttaa ctcacaaaga gaaaccacta gtgagccgtt ccagcccgga aacccaaaga 660
ttggaagctt ggggagaagg gcaagggttg acatggctga tcctgggtat ccgcttttcc 720
gcgcaggatt acttcaaata attgttaatg gcgctatacc ggccgatata cgtagcgaga 780
tggatcgctt gaatcaaata cgcgaggcgt ggaaacgtga taaaaataca agagaggtca 840
gagcgctcga cttatgcgta ctgctatcaa agattggtcg aattaagatg aatatggaag 900
aagaacctaa ggatgaggga gtcatgtcgt taaggtttca ggggaagatt gacgcgatgt 960
ttttctcgga aaatacggag aaaacaaata ttatgcgaga tggtagtggt agaactgatg 1020
atgagagatt ttacgcctta ttgctgatct gcgctacgga cgcgttctca cgacgtatat 1080
ggcgcactaa cccgtatccg tgtttgcgag gcactctaat cgcagcagag tgtgtattgg 1140
gagatccata taaaacgtta cgccgtaagt ttgactggag tgtgcgccat gcaggagata 1200
agatgctgga agggtcctcg tatgttttta caaggataaa cttattcgat acggataagg 1260
cgccaggttc acgtgtaata cactggatac aggagacact tcctgtcata aaaacaacct 1320
gggctgaggg atgttcttta gctgatgaag cgtcagatga tcatctacat tgcaaaatag 1380
atcacgcaca atatagagat ctagtatcaa gagttataaa tggagggtgg gatcaagaga 1440
actttaagat acataaatta ttcgttgaac gaggcaatat tttcttaatg gattttgaaa 1500
aggacgcaaa gattactaca cagtctgagg tggagtatcc atactattat gatacatgga 1560
tctatgcgcc gatctttagc acgaggtata agataacgga aactgaaatc gccaacgcaa 1620
aaagcttgga ccctgcgatt aagcggacgc ttgccccatt gtctgatgat cccgtagcgt 1680
tacaactgag agctgtagga catctgtatg atactcgccc tgctataatg ggtcaaacac 1740
tgtcaccacg ccagaatcag actgcactac acaagacgtt attagatgat cctgaaaatg 1800
atagatacta tgaagcacga tcgatcaaaa aagtcgataa tccttgctcc gttcattaca 1860
cgtcatcatt catattacag aagatctctg agttggtctt aagtttgatg atttatcaca 1920
ttgaacatga ctgggagcca ctgaagcaat acacgcatcc ggaaatctca acggagttcg 1980
gcgaaatatc gtgtgagata tgtgatatct ctcagctaac cgtcttatta attgacttat 2040
tctttgaaaa tagacgaacg gtacgtccaa gtgaggagac gagatatata attcatcaga 2100
tccgagaatc gagaggtgat ggcagactta agatgctaga acgattcttc cctgcctatg 2160
ggcaagctct tcgcaaaatc gagagcgcta ctgaagtggg acatataact atgttgaatt 2220
ttttgccttt tttcttttta ttgggagata acattatata taaacatagg gagtggactg 2280
ttccaatcct aatatacact gatggcgtca tgatttggcc cgcgcaggta ggcgctaatt 2340
ataatagatt tggtttctgc ggttttctta attatatgag gttccatgct ggaagccaac 2400
tgagatctaa agtactcgat gatgctgaga aaaccattgc aaaagaaatg cttaagtact 2460
atatgaaaac cgacattttt agtggaggcg ttcaaaagaa tatacgtgtc acgaaatcaa 2520
tgcaaatgga aatataccac tcatcgctgt gtggaggatt atcagacgca gtggtatatg 2580
cattgccaat tagcttcccg atcaaatgtt tgactttgat tattattggt gatgatctcg 2640
ttgaaccgca actcagaatc tcgagagtta tggattattt taagcatgtg agcgagcacg 2700
tccgaggcgt tgcatcgatc tcaatttcga gaaatggaga tgtttctacc tattctcgcg 2760
gtattgtgca tgtcgagttg ttgaagaaaa atatattaaa ataccaattt caagtcgctt 2820
tactaaaagt taaaggatac gtgtttggca atgatgaaat gctcattaaa ttattgaacg 2880
tttagtctca gtgacatgag actcctggag catacactta c 2921
<210> 88
<211> 1637
<212> DNA
<213> 人工序列
<220>
<223> BTV5 AJ586700的VP5 DNA
<400> 88
gttaaaaaga tccctatgat cgcggaagat gggcaaaatc ataaagtcat tgagccgctt 60
tggcaaaaag gtggggaatg cgttgacatc gaatacagca aagaaaatct atagtacaat 120
cgggaaggca gcggagcggt ttgcagaaag tgagattgga tcagcggcaa tcgacggatt 180
agttcagggt agcgtacact cgatcatgac gggagaatca tacggtgaat cagttaaaca 240
ggcggtactg ctaaacgtgt taggtgcagg agatgaaatt ccagacccac taagtcctgg 300
cgagcgtggg attcaaatga aaattaaaga aatcgaagaa gaacagagaa atgagttagt 360
gagattaaag catgggaaag aaataacaag aaagttcggt gcagagttag aggagattta 420
ccaatttatg aatggcgaaa tgcgagaaga ggcggagcag gaggaacagt ataaggtgtt 480
gtgcaaagct gtcaattcat acgaaaaatt actcgtcgcg gagaatgatc agatgcgcgc 540
gttagcacga gcgttacagc gtgaagcgac tgaaagaaca gaagcggaat ctacgatggt 600
aaaggaatat agacaaaaga tcgatgcatt aaaagcggct attgaagtcg agagagacgg 660
gatgcaggag gaggcgatcc aagagatagc aggaatgacc gcggatgtac ttgaagccgc 720
gtctgaggaa gtaccgttag ttggttctgg gatggcgacg gccatagcga caggtagagc 780
aatagaggga gcgtataaac taaaaaaggt aataaacgcg ttgagtggga ttgatctgtc 840
gcacttgcgc acgccgaaaa ttgaaccgac catggttgcg actacattag agcacagatt 900
tgatgagata cccgataaag aattggcggt gagcatatta gccaaaaata acgcgattgt 960
tgcgaataca agagaagtgc aacacataaa ggacgaaata ttaccaaaat ttaagaagat 1020
tatggatgag gagaaagagc ttgaaggcat tgatgataaa aagattcacc cgagggtgat 1080
gatgagattc aaggtgcctc gctcccagca gcctcagata catatatata gcgcaccgtg 1140
ggattcagac gatgtcttct tctttcactg tgtgtcccac tttcacgcaa acgagtcatt 1200
ctttttggga tttgatctgg gcatcgatgt cgtacatttt gaagatttgg ccgctcactg 1260
gcatgcgcta ggtgcggcgc aagaggcgaa ggggcgtacg ctcagtgagg cttatcgtga 1320
gtttttgaat ttatctattg gtagcgcgta cacatccccg atgcacgctc gacgcatgat 1380
taggtcgaaa acagtacatc ctatatatct cggttcgatg cactatgaca taacgtatga 1440
aactttgaaa tcaaacgcgc agaggatcgt atatgatgac gaattgcaaa tgcatatctt 1500
gagaggtccg ttacactttc aaaggcgcgc gattttaggt gcacttaaat ttggggttaa 1560
ggttctcggt gacaagattg atgtgccact cttcctcaga aacgcgtgac tgcggtgatc 1620
ccgggacttc cacttac 1637
<210> 89
<211> 2922
<212> DNA
<213> 人工序列
<220>
<223> BTV6 AJ585127的VP2 DNA
<400> 89
gttaaattag tttcgtgatg gatgagcttg cgattccgat tattaatggg catttcccca 60
aagaagcact aattgagtat gactgcatag tagaattgaa tgcgccgagc gaagatgatg 120
aggtcgatgt aacccaaatt cccacgaaga acatgatgga tataccggat gttccgattc 180
gtgaagcgct aagggtttta cctcaaagga atgatggtca cgtgctaaca cggatactgg 240
atatatcgtt aagcggatat gataggagga aatattataa gacaaataaa ggcggtgagt 300
ttgaatcgcc gaaggagtgg ctaaattgga tgatccacga ctcaatggac gtgcaacctt 360
taaaggttag tttggaaatg agaccaacag ttaaacatga attgttcagc tcaaacgtat 420
atctatcagt gaagaaggca gatacatctt catatcacat cgaggcgatt ggtgacacag 480
gtaaggcgtg cgatcattcg tacgtatcta tgtggaataa cttagtgagg cacgaagtaa 540
tgcactgcgc acaggagagt gcctatgtcc tgaaaccaac tatggagata attgtacact 600
ctgagagagc ctcgactgac gtaccgtttc agctggttgg acagaattac gtttcgatcg 660
ggagaaatca tcgattggta ttaggagatg agccatataa gaagacatta cagggattga 720
cgaaattgcg tgtgcagggc aaagtgccac ctcagataca ggatgaaatt cagcagctaa 780
ataggatacg tgatgcgtgg attggtgatg cgtacaatcc gaggcatatt cgctcgctgg 840
aactatgtcg gattctgtca agtgtcggaa ggaaggtggt atgtatagag gaagagccga 900
agaacgaaag tgatatatcg gtcaaattcc aatttaaact agatgaaaaa ttcagattaa 960
atgatggcga gcggggagtg atatttacgt cgaaaaacca aaggaatgat caggatcggt 1020
tctttgtact aattatgata gccgcctctg acacctataa tggcagaata tggtggacaa 1080
atccgtaccc ttgcttgcgt ggagcattga tcgcatcaga aacgaggttg ggcgatgttt 1140
actggactct aagacacact tacgattgga gcgttagaat gacatactcg ccgaggatga 1200
aagaacgtga agatgacaag tacatatatg gacgcgtgaa tttattcgat ctagatgcat 1260
cacctggtga taaagtcgtt cattggaggt atgagttgat ctcacagacg aagaagacta 1320
cgtttcatga tggtaatcct tgtgacttat ttccggatgt agaaggaatt gtgactaaat 1380
ttaacaagac cgcatattca aacatgatca acgaattact aaatgggggg tggaacacac 1440
gcgaatttaa gatgtacaaa ttgttggaag atgagggaaa cgttttgaca atagatcttg 1500
agaaagatac gaagcttaat tcgagatcgg agtttattct acccaattat tatgataagt 1560
ggatatatgc tccaatgttt aacgcaaaat tgaagattac ggagaccgag atagcgcagg 1620
caaagaatga cgatccatac gtcataagaa cgcttgcacc cataggctca aacccattca 1680
ctctacaacg gttgtgcctt ggaaattact atgatgtacg acctgctatg catggacgcg 1740
cgctctctaa gaaacaggaa cagtcggctt atcacgcggc tctcgtgaaa gagaaggatt 1800
acgaagacat actgacacgc cggggtttgc gtaaagctga caagccgtgt ccattaacca 1860
cgtgtgctta tattctcgag aagacagctc tattgtggat tgatgtgatg gagaagcaca 1920
ttagcgaagt cactgatgat tcaaacgaat tcgattttcc aaaagcgtgc gtggattcac 1980
cagttgggac tgataggatt gtcgatgtca cacagttgat cgtacttctt atagactgga 2040
tttatgagag acgtaaagat gttcgtagtg tggatgaatc aaggtgggtc atagaaacga 2100
ttagacgtgc tagaggtaga aatagattag aattaatatc acgtgagttt cctaactttg 2160
gaagagcagt tgcacgccta caatcaccaa atactgtagg tgatgttatg gtaatcaact 2220
ttttaccctt cttattcttg atgggagata acgtctcgta tgaacatagg caatggtctg 2280
tcccggtcat tctgcacgcc gacgttatgt ggtttattcc ggttgaagtt ggggctttct 2340
acaatcgctt cggcgtgacg tcattcctag aatatttgat gttctttccc tctttccaca 2400
tgaggaccgt tcgactagat gaggctgaag tgaaggtaag caagttaatt ttagacttct 2460
atcttgatac gacaatattc tctggtggca tacagagtag cgtggtctcg actaagcaga 2520
tcttgtatga aacatacttg tcttcattat gtggtgggtt ctcggaggga ttagtttggt 2580
atcttccaat cacgcatcct acaaagtgtg tagttgcgat cgagttcgcg gacaataaag 2640
ttagcgcggt ttataggtgc aatagattac gcgcgaggtt ccctttgagt tctatacact 2700
taaggggcat tgcgatcctt acgatcggga agaatagaat ggttgacgct tacacagagg 2760
ggatcgtctc tcatcgtata tgtaagaaga acgtattagg atatacatgt cagattctat 2820
tgctgaaatt ttcaggccac gtttttggaa atgacgaaat gctgacgaag ctgctcaatg 2880
tttaacctca gtgacatggg gttcacgaac taatcaactt ac 2922
<210> 90
<211> 1637
<212> DNA
<213> 人工序列
<220>
<223> BTV6 AJ586703的VP5 DNA
<400> 90
gttaaaaaga tccccatgat cgcgaaagat gggcaaaata atcaaatcgc taagccgttt 60
tggcaaaaaa gtgggcaatg ctttaacctc aaatacggcg aaaaagattt atagtacaat 120
tggaaaggcg gcggagcggt ttgctgagag tgagatcgga tcagccgcga tcgatgggct 180
agttcaaggc agtgtgcact caattatgac gggagagtct tacggtgaat cggtcaaaca 240
ggcagtactg ttgaatgtac taggagcagg ggatgagatt ccagacccgt tgagtccggg 300
tgagcgcggc attcaaatga agattaagga gatagaggaa gagcagagga acgagttagt 360
gagactgaag catgggaaag agatcgcgaa gaaatttggc gatgagttgg aggaaatata 420
tcagtttatg aacggcgagg tgaaagagga agttcagcag gaggagcaat ataaggtgct 480
ctgtaaagcg gttaactcgt atgaaaaatt gcttctagcc gaaaatgacg agatgcgtac 540
gctagcgcgg gcgctacaac gtgaagccac tgaaagaact gagaccgaat ccaagatggt 600
gaaagagtat aggcaaaaga tagatgcatt aaaagcggca atcgaagtgg agagggatgg 660
gatgcaagag gaagccatcc aggagatcgc gggtatgaca gctgacgtat tggaagcagc 720
ttctgaagag gtaccgttag tgggttcagg aatggcgacg gccatagcaa cgggacgagc 780
gattgaaggt gcatataaac tcaagaaagt cataaatgcg ttaagcggaa tagatctttc 840
gcacttacga accccgaaga tagagccgac gatggttgca accactttag aacatagatt 900
cagagatata ccagataaag aattagctgt aagtatcctg gcaaagaata atgcgatcgt 960
agcgaataca agagaggttc aacatattaa ggaagaaatt ctaccgaaat tcaagaaaat 1020
aatggatgag gagaaagaat tagaagggat cgatgataag aagatacatc cgagagtaat 1080
gatgaagttc aaggttccac gatcgcaaca gcctcaaatt cacatttaca gtgcaccatg 1140
ggactctgat gatgttttct tttttcattg cgtgtcgcat tttcatgtta atgagtcttt 1200
ctttctaggt tttgatctgg gaatcgatgt tgttcacttt gaggacttag ctgctcattg 1260
gcacgctttg ggagccgctc aagaggcgaa aggacgtact ttgaatgaag cgtacaagga 1320
atttttgaac ttatctatcg gcagcgctta tacgtcacca atgcatgcga ggcgtatgat 1380
tagatcaaaa acagttcacc cgatctacct aggatccatg cattatgaca tcacttatga 1440
gacgttgaaa tcgaatgcgc aaaggattgt ttatgatgat gaattgcaga tgcacatatt 1500
gagaggaccc ctacatttcc agcgacgcgc aatattgggc gcgctcaaat tcggagtgaa 1560
agtgcttggc gacaagatag atgtgccact ctttctaaaa aacgcgtgac agcggtgatc 1620
ccgggactta cacttac 1637
<210> 91
<211> 2936
<212> DNA
<213> 人工序列
<220>
<223> BTV7 AJ585128的VP2 DNA
<400> 91
gttaaaaagg acctcgccag aatggatgaa ttcgcaattc caatcgtgca tggcagagtg 60
attccgtatg atttggtgcg ttcgtatgac attgtagtgg atttagatgt gagaatagat 120
gaggaaggag acaaacatga tacgaccaag ataccagaag catatatgtg tgatgtaccg 180
aatatagcga tgcaggatgc taattcagca atattgggga ggaatgatgg ttacgctttg 240
ccccgcattt tagatgtatc catagcggca tatgatccta ggaagggata ttggaaatat 300
agaggtatag agaagatgac taatcaaacc tggttgaagt gggctatcaa ggattttatg 360
gatgtgcagc cactgaaggt ggatctggct gatcttgaag gtaaagtgag gtatgatatg 420
tttaattgtt ctgctcgaat agatgctaaa aaagccgagg cgatttatta tggttgggca 480
gctcaggagg tagtgaacaa aggttgtaac catgcgaaaa gcgagatgtt tcagcatgtc 540
gtaagatgcg agctgttgaa ttgttcgcag gaaagcgcgt acattttaaa gccgactcaa 600
aagatagaac ctctcgctga aaagaatgat ctgggtgaga ggctcgggcc aggctctaat 660
aacttaatta atatgcgacg tggggataca tttaggatgg ggacagacca gtacaaccgc 720
atgttgcagg gatggtaccg ggtgaaggtg aaaggtcaaa ttccggatat ggttagagaa 780
gagatggctg agctagtaaa gataagggat agatggaaag ttgcgaaaaa tccgagagag 840
attaaatccc ttgaattatg taaattgctc tccacgatag gtaggagaat gtataatatg 900
tacgaggaac ccaaagatga ggaagcggtt tcgttaagat ttcagtggaa tttggatgag 960
attttcaaaa ctgataatag ggagcacgtt aacattgctg tcaacaagaa tgggagaaca 1020
gatgatgagc gattcttcgc acttatcatg atagccgcaa ccgatacgaa tcgcggaagg 1080
atttggtggt cgaatatgtt tccgtgccta cgaggcgctt taattgcgag cgaaactcaa 1140
ttcggcgatg tatataagat gctaagggtg cgattcgaat ggagtgtgcg gaatgaatat 1200
agccctcagg ctgacgtcgc aagacagaat gagaactacg tgtacgcacg cgtcaatcta 1260
tttgatctag tcgctgatcc ggcgacatct gtgatccatt gggaatataa attggataag 1320
ccctttaaga cttgctactc agacggtcat caatgtgatg aatatcctga tgaatatgat 1380
ttaatttgct cctttgatga agctaaatac agcgaaatgg tccaacgcat tatagagggt 1440
ggatgggatc ataaaaactt taaacattac aagatattga aagaagcttc aaatgtgctc 1500
acgatcgact ttgagaaaga tgcgaagttg aatgagagct cggagatgaa cctgccttat 1560
tattttaaca aggacatcgc ggcgccaatg tttagatgtg tgttgaaagt atgtgagacg 1620
tcaatctcag agcgccacaa tctagacccg gcaatatcgc gtacgatgaa acccatgagg 1680
gctaaagctg cggacctatt agactactct ctgggttcct actatgagac ccgatctggc 1740
gtgatgggca ggacgttaac aacaacgcag aagcagagtg atatgtataa gatggttcaa 1800
gacgaagatg attacttatc attgatggta ggacgaggcg tcaccgacaa atttgcgaac 1860
ccatgcccta ttacaactgc tggccagtac gctctacaaa agacatcagt tatgatcttt 1920
tcacgggttg aacattattt tgaagagtat ctcaaagatg acaaggtaga gtatttgagc 1980
ccgtctctaa accttgaatg ggttgaaata acatcaagga taacggatat ctcgcagttg 2040
atagtactga cgtttgatgt attttttgaa gggcgtagac aggtgcgtac tattgatgag 2100
tcacggttta ttctctatca gataagggcg cgtaaaggta tggatcggct ttcatatatt 2160
aaagatctat tccctcaatt atatgggtgg tgggtgtctc cagagcccac atatggccac 2220
gctcagaaat taaatcttct acccttgtta ttcataattg gtgataactt tatttatgag 2280
catcgccagt ggtcataccc ggtgatttta gtgtcacaca aactgagatt cgtacccgtt 2340
gagttggggg cttattacaa cagatttggg cttacgtctt tattctctta cttggttttc 2400
cacgtaggga agtcaaaata tgggttggaa cttgatgaag cagagaaaaa ggtgctgcag 2460
gctgcgaaag actattatgc tgtggcgact atttcagagg gagggattaa tacgcattca 2520
actatgacca aagatccgat ttatcaagtt tatctatcaa cgatatgtgg cgggttactc 2580
gattcgctta tctatctgat gccgattacc caccctgtca agagtatcat catggtaatc 2640
ataaatgatg ggagggggtc ggcctcggac tgcgtacggc gtgcgacacg tcgattgaga 2700
tatattcaac gtcatatacg cggctcggtt gtcgtctcgg tcttgcggga tggcgtgatt 2760
aatacatatt cagaggggat cgccaaagcc atgttgtgcg agaaaagtgt tttgaagtac 2820
aattataagg ttgtgctggt taaggtgaaa ggatatgtgt ttggccatga cgaaatgcta 2880
actaaactgc tgaatgtata atccctttga caggggattg gcggccttaa acttac 2936
<210> 92
<211> 1637
<212> DNA
<213> 人工序列
<220>
<223> BTV 7 AJ586704的VP5 DNA
<400> 92
gttaaaaagt gtcctcatca tcgcagagat gggcaagatc attaaatcat taagtagatt 60
cggaaagaaa gtggggaacg ttctgacgtc aaatacggcg aaaaagattt atagtacgat 120
tgggagggcg gctgaacgtt tcgccgagag tgaggtgggt gcagccacga tagatggaat 180
tgttcaggga agcgttcact cgatcatgac gggcgagtcc tatggtgaat cggtaaaaca 240
ggcagtactg cttaatgtgc taggcagtgg tgaagaatta cctgaccccc taagtccagg 300
agaacgcgga atacagacca aactaaaaga gttggaggat gagcaacgca atgagctggt 360
ccgtttgaaa ttgaataaga aaatagtcga caaattcgga acagaattgg aggaggttta 420
tgattttatg aatggggcgg cgaaagatga tgagaatgaa attaaacaat acgatatttt 480
gtgtaaagct gtagactcat acggaaagat cttgcgagag gaagacaaga aaatgttgga 540
attagcgaga gcgttgcagc gtgagtcgga gtcgagaagt gacgaggaga ccaagatggt 600
gcgggaatat cgttcgaaaa tcgatgcgtt acgcgatgcg gttgacgttg aacgtgaaag 660
catgaccgag gaagcgatac aagagatagc aggaatgacg tcagatgtcc tagaggcggc 720
ggcggaggaa gttcccttgg ttgggtcggg aatggcgacg gcaatagcaa cgggacgcgc 780
gattgaaggc gcgtataaat tgaagaaggt aataaacgcg ctaagcggca tcgacttgac 840
tcatttacgc acgcctaaga tagagcccag cttaattgct acaacattag agtatagaga 900
taggccaatt ccagatgatt cactagcagt cagcgtactt gagaagagga gggcgataga 960
agaaaataaa aatgagatcg aacatattaa aaatgagatt ttaccccggt ttaaaaaagc 1020
gatggaggaa gagaaggaga tttctggaat cgaggagtct gtgatacacc ctaaagtaat 1080
gatgagattc aagatcccca gatctcagca gccccagatt cacatttatt gttcaccgtg 1140
ggattcagac actgttttct tttttcattg tgtatcacat catcatatga acgagtcctt 1200
ttttatcggt ttcgacttag agctagattt agtgcatttt gaagatttga ccgcgcattg 1260
gcatgcttta ggcgccgcgc aaatggttgt tggacgcacg ttccaagagg cgtatcgaga 1320
gtttttaggt cgtgcgatga tggatccctc gagcggagcg atgcataaga gaagaatgtc 1380
acgatcccgt agtgtgcatc ccatttatct tggatcgatg cactatgata tatcctacgc 1440
taccttgaag agtaacgcac agcggatagt ttatgatgat gagttgcaga tgcacgtgct 1500
gcgtgggccg atacattttc agcgcagggc aatcttaggt gcacttaaac atggcattaa 1560
aatacttaat gataaaattg atctcacatc tcttcttaga aatgcgtgaa gcagcgatga 1620
caggcgctta cacttac 1637
<210> 93
<211> 2939
<212> DNA
<213> 人工序列
<220>
<223> BTV8 AM498052的VP2 DNA
<400> 93
gttaaaatag cgtcgcgatg gaggagctag cgattccgat ttatacgaat gtattcccag 60
cggagctttt agatggatat gattacatca ttgatgttag tagtcgtgtt gaagaggaag 120
gtgatgagcc cgttaagcga cacgatgtaa cagagattcc taggaattca atgttcgata 180
ttaaggatga acatatacgt gatgcgatta tatacaaacc agtcaacaat gatgggtacg 240
tactaccgag agtattagat atcacgctga aagcctttga cgaccggaaa agagttgttt 300
tgaatgatgg tcatagcgaa tttcatacga aaacgaattg ggttcagtgg atgattgatg 360
acgcaatgga tgtccaacct cttaaggtag atatcgctca cacgcgctcg aggatcagcc 420
acgcactctt taactgtacg gtgcgattgc attcaaaaaa ggctgacacc gcatcttatc 480
acgttgaacc agttgaaatt gaatcgtggg gatgtaacca cacatggttg agtaggattc 540
atcatctagt gaatgttgaa ttatttcatt gttcacaaga ggcggcgtat acattgaaac 600
ccacatataa gataatatca aatgcggaac gtgcatcaac gagtgattcc tttaatggaa 660
ctatgatcga attaggtcgt aatcatcaaa ttcagatggg tgaccaggga taccagaagt 720
tgaaagaagg gttggtacaa gttcgaattg aagggaagac gcctttggta atacaagagg 780
aaataaccgc tttgaataaa ataagagaac aatggatcgc tcgaaatttc gatcaaaggg 840
aaatcaaggt tttagatctg tgtaggttgt tgtctacgat aggtaggaag atgtgcaata 900
ctgaagagga acctaagaat gaagctgatc tttcagtgaa gttccaaatg gagcttgacg 960
aaatatttcg accgggaaat aacgagcgta ccaacatcat ggggggcgga gtacatcgga 1020
aaaacgagga cagattttac gtactaatta tgatcgctgc atccgatacc aataaaggtc 1080
gtatatggtg gagcaatccg tatccctgcc tgcgtggagc gttaatcgcc gctgaagtcc 1140
aattgggtga tgtttacaat cttttaagga attggttcca atggagcgta cgacctacct 1200
atgtacctta cgataggaat agagaaagtg ataagtatat ttatagtaga atcaatttat 1260
ttgattcaac cctgagacca ggcgacaaaa tagtacattg ggagtataag ttgcttaatg 1320
aagtacgaga ggttagcatc aacaaaggta atgagtgcga tctgttccct gaggatgaag 1380
aatttaccac caagtttcat gaggcgcggt atacggagat gaagaaccaa attattcaaa 1440
gcgggtggaa tcaacgagat tttaaaatgc ataaaatact agaggatggc gcaaatgtgt 1500
taacgattga ttttgagaag gacgcgcaca tcggcacggg ttcggccctg agtttgccgg 1560
attactataa caaatggata attgctccga tgtttaacgc taagttacgc attaccgagg 1620
ttgtgattgg aacggctcac accgacgatc cagctgtagg gcgcagcgcg aaggcgttca 1680
cacatgatcc ttttgacctg cagcgatatt gtctagcaag atactatgat gtacgacctg 1740
gtatgatggg ccgcgctctc tctaagcaac aaaatatgtc gtcgatgact gataaactct 1800
ctaagcagga ggattacgca gggattgttt caaggcgtct ggaatacaag gaacgagaga 1860
acagatgctt gacggagaca gcgcagtatg tattcgagaa aacatgctta tacgtactag 1920
aattattgag cagacatacg atgccctcgg aggattctga agtaacgttt gaacacccaa 1980
caattgatcc cagcgtcgat attgaaacgt ggaaaataat tgatgtttcg caactgatca 2040
tattcgtatt tgactatttg tttgaaaatc ggaagatcgt gagggatact accgaggcaa 2100
ggtggacgct tttcaaaata agatcggaag taggacgagc caggattgac gcaatagaga 2160
tgacttttcc tcggtttgga cgtatgctaa gaaatgcgtc tcaggcaaag ataaatcaag 2220
atatcgcgtg cttgaacttc cttccactac ttttcatcat aggcgataat atatcttacg 2280
cacatagaca gtggagtata ccggtgctgc tgtatgcgca cgatatccga attatacctt 2340
tggaagttgg agcgtacaat aatcgttttg ggctgacgtc atacctagag tatatggctt 2400
tctttccatc gtacgctact agagtagcta aaattgacga gtcgatcaag gagtgcgcga 2460
tcgcaatggc tgagttttat atgaacaccg atatccacag tgggagtgtg atgagtaacg 2520
tgataacgac caagcggcta ttgtatgaaa catacttagc ttcgctatgc ggcggttatt 2580
cggatggatt gctttggtac ctgccaatta cacatccctc gaaatgctta gtcgcctttg 2640
aggtagctga tgacgtagtt ccactctcag taaggcggga acgtattcta tctaggtttc 2700
ctttaagctc gagacatgtc aaggggattg cgttgatttc ggtggatagg aatcaaaagg 2760
tatcggttca aacagagggt attgtaacac atcgtctctg taaaaagaat ctgttaaaat 2820
acgtgtgtga tgttattctt tttaagtttt ctggacacgt ttttgggaat gatgagatgt 2880
taactaagct gctcaatgta tagctctcgt gactgagagc tcgcgcgcta tcaacttac 2939
<210> 94
<211> 1637
<212> DNA
<213> 人工序列
<220>
<223> BTV8 AM498056的VP5 DNA
<400> 94
gttaaaaaag cgatcgctct cgcgaagatg gggaaaatca taaagtccct aagccgattc 60
ggaaagaaag ttggaaacgc cttaacatca aacacagcaa aaaagattta tagcacaatt 120
ggaaaagcgg ctgaacgatt tgcagaaagt gaaatcggct cagcggctat tgatgggtta 180
gttcagggga gtgtacattc gttgatgacg ggagagtctt acggcgagtc ggtaaaacaa 240
gctgtgctat taaatgtaat gggaagtggt gaagagcttc cagatccact aagtccgggt 300
gaacgtggaa tgcagacaaa gatccgtgaa ttggaggatg aacagcgtaa tgagttgatt 360
cggttgaagt ataatgataa gataaagcaa aaatttggga aagaattaga agaggtatat 420
gagtttatga atggggttgc gaagcaggag gaagacgaag agaaacatta tgatgttctc 480
aaaaaagcgg taaactcgta cgataaaatc ttaaccgagg aggaaaaaca aatgaggatt 540
ttagcaacag cgttacaaaa ggaggtgaag gagcggacgg ggacggaggc cgtcatggta 600
aaagagtatc gaaataagat cgatgcgtta aaagaggcca tagaggtaga gcgtgatgga 660
atgcaggaag aggcgattca ggagatagct ggaatgacgg cggacgtatt ggaggcggct 720
tcggaggaag tgccactaat aggtgcgggg atggcgacag ctgtagctac aggtagggcc 780
atcgaaggag cttacaaatt aaagaaggtt ataaacgctc tcagcggaat cgatttgact 840
cacttaagaa caccgaagat tgagcctacg atagtatcga ctgtgttgga tcataagttt 900
aaggatattc ctgatgagat gctcgcggta agcgttttat caaagaatag agccatcgaa 960
gagaaccata aagagattat acatcttaag aacgagatat tgcctagatt taagaaggcg 1020
atggacgagg agaaggagat atgtggaatc gaagacaaaa agatacatcc gaaagtcatg 1080
atgaaattca aaattccgcg aactcaacag ccccaaatcc atatatatag tgcaccgtgg 1140
gattccgacg acgttttctt ttttcattgt atatcgcatc accacgcgaa tgaatcattt 1200
tttattggat ttgacttagg catcgatctg gttcactacg aagatttgac tgctcattgg 1260
cacgctttgg gtgcggcgca ggcggcggtt gggaggagtc tgaatgaagt gtacaaagaa 1320
tttttgaacc tagcgattaa taacacgtat agctcgcaaa tgcacgctcg gaggatgata 1380
cgatcgaaga ctgtacaccc catatatcta ggctcactcc attatgatat ttcgttttcg 1440
acattacgga gcaatgcgca gagaattgtg tatgatgagg aattacagat gcacatattg 1500
aggggaccac tacatttcca aaggcgggcg atactaggcg cgataaaaca tggagttaaa 1560
atcttaggga cggaggttga tatcccactc ttcttaagaa atgcctgagc gcagcggagc 1620
caccgctttc cacttac 1637
<210> 95
<211> 2921
<212> DNA
<213> 人工序列
<220>
<223> BTV9 JN255903的VP2 DNA
<400> 95
gttaaaagtt atctaggatg gatgaattcg gaatacccat ttatctttcg agtagcgtac 60
cacacgactt actcagcgat tacgatttta tgattgatct gagtgataaa gtagtgcacg 120
ccggagaccg acatgatccg aaaaaattga aagagaggaa agcaatagat gtgcctgatg 180
gtgaagcgcg tacagactta aattataatc caacagataa tgaaggtaat caactaccac 240
gagcgctaga tatcgcatta agtgcgcgtc ttacgagaaa gagcgtgcga aataatgatg 300
gcttggactt tcttaccgat gataaatgga tggagtggat gctatctgac gctatggatg 360
ttcagccact aaaaattgat ttgagtgcgc gaaatggatt tgttaagtgc gacattttca 420
actcatctat atatataagg agaaaatacg ctgatgcgat cgcgtatcga tatacgtcat 480
tggaagatga atcaaagggt tgcaaccata cgagagtgtg tatggttaac catctgataa 540
ataatgggtt atataacgct gtgcaagagt gtgcttacgc tttaaaggat acgtacgttc 600
tacgagtgca ttcacagagg gaaaggatcg atgaaccatt cgagccaggt aggccgaaga 660
taggtagttt ggggaggaat gcgagaatag atatgagcga tccggggtat tcgcttttta 720
aaggtgggat gttacagatt acggtaagtg gtgagatacc gaatgacata cgcgttgaga 780
tggaacggct aaaccaaatc cgtgccactt ggatacgtga taagtcttca agggaggtga 840
gggccataga gttgtgcaca ctattatcaa ggattgggcg tgtaaaatgg aatgcagagg 900
aagaacccaa ggatgaaggt gctatgtcat tgagatttca gaacaaaata gattcaatgt 960
tcctctccga gaatacagag aaaaccaata tcatgcaaaa cacgagtggt cgcacagatg 1020
aggaaaggtt ttatgccttg ttgctgatat gtgcaacgga tgcgtactca cgtcgtatat 1080
ggaggtccaa tccatatccc tgtctacggg gcacgttgat tgcagcggaa tgtgtcttag 1140
gtgacccgta taaaactttg cgacggaaat ttaattggag tgtacgccat gcggcagaca 1200
aagcgctaga gaataacgcg tatatcttta caagaataaa cctgtttgat acagaaagaa 1260
caccaggcat gcgggttgta cattggacgc aggagctaac tatggagtcg cagacgactt 1320
gggacgaagg atatccattg aaagatgaag cgcccgatga tgagatgcac tgtaagattg 1380
atacaaacaa atataaagaa atggtgacgc aggtgattga tggtggatgg gaccaggaga 1440
atttcaaaat ccataaactc tttcacgacg atgggaatat atttctgatg gatttcgaaa 1500
aagatgcgaa gttgaccgca cagtcggagg tgcaatatcc tgactactat gataagtgga 1560
tttatgcccc aatctttgac actaagtaca aaatcacgga gacggagata gcgaatgcta 1620
aaaatctaga cccggcaatc aagcgcacga tagatcctat gatggatgac cctgtcatgt 1680
tgcaattgag tactataggg aatctgtatg ataccaggcc agcggtgatg ggatcaaccc 1740
tatcaatgag acaacgccag acatcgcttc ataaaacgct attggatgat cctcaaaatg 1800
aacgtttcta ttcatctaga tcaatcaaga aagttgacga cccttgcccg gtttattata 1860
ctgcatcttt agtgttgcag aaaatctctg aacttgtaat gagcttgatg atctaccatg 1920
tggaccagga ttgggagtca cttaaacagt ataaacatcc tagtattctg tccgactttt 1980
cttcgctcgt gtcaaatatc caggacatct cacaattggt tgtcctctta gttgatttct 2040
tctttgaacg aaggagcgtg ttaagatcgg ttgaggagtc aagatacatc atacacatca 2100
ttcgctcgtc aaacgatgcg aatagattga agagttttga attatatttt ccacgatttg 2160
gagggatatt acgaacgatc aaagatgcta aagaggtttc tcacgttacc gctttaaact 2220
ttttacctta ttttttcttg ctgggagata atataatata taagcataag gagtggtcgg 2280
ttcccgtgct aatatattca gatggcctca tgatatggcc tgcgcaggtt ggagctaact 2340
ttaatcgatt tggctttttt ggattcttga attatatgag atttcatccc gggattagat 2400
tgagatctaa agtgctcgat gatgcggaaa aagttgtagc ggttgaaatg ctgaattact 2460
atatgcgtac cgatatattc aaaggaggta ttcagaagaa cgtacgagtt acgaaactgt 2520
cgcaaatgga aatctatcat tcatcattat gtggcggtct atcggatgct ttaatctatg 2580
cattgccaat cagttttccc atcaaatgtc ttactttaat cattgtcggc gatgatttag 2640
ctgagcctca acataggatc agcaaggcct tggaattttt tgagcatgtc cgcgaccacg 2700
tgcggggaat cgcgtcaata tctatatcta ggaatggaga tgtaacgacg cattcgcagg 2760
gtattgttaa agttgagctg ttaaaaaaga atatcttaag gcatcagttc cgagttgctt 2820
tattgaaggt gaaaggatat gtatttggta acgacgaaat gttgactaaa cttctgaatg 2880
tatagtccca gtgacatggg actcctagag cttacactca c 2921
<210> 96
<211> 1635
<212> DNA
<213> 人工序列
<220>
<223> BTV9 AJ586708的VP5 DNA
<400> 96
gttaaaaagc gtacccttag cgaagatggg taaagtcata cgatccctaa gtcggtttgg 60
taagaaagtt ggaaatgcgc taacgtcgaa tacagcaaaa aagatttaca gcacgattgg 120
aaaggcggct gaacggtttg cagaaagtga aatcgggtcc gcagcgatag atggcttagt 180
gcaagggagc gttcattcaa taataactgg tgaatcatat ggggagtcag taaagcaagc 240
ggtgttatta aatgttttag gaagtggtga ggaaattcct gacccattga gcccaggcga 300
acgtggaatt caaacgaagt taagagaatt agaggatgag cagagaaacg agttggttcg 360
tcttaaatat aatgatgaaa ttaaaaggaa atttggggaa gagttagaac aagtttacga 420
ctttatgaat ggggttgcga aggacgagga gggcgaagag aaacagtttg atatattgaa 480
caaggcagtg aactcatatg acaaaatcct agcggaggaa gatacgcaaa tgaggcgttt 540
ggcgacagct ctacagaaag agattggaga tagaacgcac acggagacga cgatggtgaa 600
ggaatataga agtaagatcg aggctttgaa gaacgcgatt gaagttgagc gagatgggat 660
gcaagaagaa gcaatacagg agattgcggg tatgaccgct gatgtgttag aggctgcttc 720
agaggaggtt ccattgattg gagcaggaat ggcgacagcc gtagctacgg ggagagcgat 780
tgagggagct tataagctca aaaaagttat aaatgcgctg agcggaattg atttgactca 840
tctacgcact ccgaagatcg aaccgagcgt tgtatctacc atacttgagt atcgtgcaag 900
ggatgtaccg gataaagcgt tggctgtaag tgttttatcg aaaaatcgga caattcaaga 960
gaaccacaaa gaactaatac atataaagga cgaaatcttg ccaagattca aaaaagcgat 1020
ggaagaagag aaggagattt gtgggataga ggataaaacg atccatccaa aagtcatgat 1080
gaaatttaaa atacctagaa cgcaacaacc ccagatacac atatatagtg cgccgtggga 1140
ttcagatgat gtcttctttt tccattgtat ctcacatcat catgcgaatg aatctttctt 1200
tcttggattt gatttaagta ttgatctagt tcactatgag gacctgaccg cgcattggca 1260
cgctttaggg gcagctcaaa cagcggcagg acgtacgctt acggaggcgt ataaagaatt 1320
cttaaacttg gcaatttcaa ataattacgg tactcagatg cacacgcgga ggatgataag 1380
atctaagacg gtgcacccca tttatttggg atcgctccat tacgatgtgt ctttcgcaga 1440
cttacgtgga aacgcgcagc gtatagtata cgatgatgaa ttgcagatgc acatattacg 1500
gggaccacta cactttcaaa ggcgagcgat cctgggtgcg ttaaagtttg gatgtaagat 1560
cttaggggat caaatagaca taccactctt cttaagaaat gcttgaacgc agcgacggga 1620
agcgcttaca cttac 1635
<210> 97
<211> 2921
<212> DNA
<213> 人工序列
<220>
<223> BTV9 AJ585130的VP2 DNA
<400> 97
gttaaaagct tttcaggatg gacgagttcg gtattccatt gtatcgttcg actcatgtgc 60
ctcatcacgt tttagaagat tatgaactta gaattgattt gagtgatcgg gtcgtacatg 120
gaggggatcg tcacgatccg aaaaaactga aagagcgtaa agcaattgat gtgccagatg 180
gtgatgtcag gttagactta aactacaacc ccactgataa tgaagggaat cagatacctg 240
gagctcttga tatcgcctta tgcgctcgga tcacaaggaa gagcgttaaa acgaatgagg 300
gtgttacgtt tacaaccaac cataagtgga tggaatggat gcttttagac gcgatggacg 360
ttcaacccct gaaaatcgat tttgcagcgt taggaggcgc ggtcaagtgt gacttgttca 420
attcagccat atatgttaaa aagaaacacg ctgatgcgat aatgtacaga tatatcgcgg 480
tcgaagacga atcgagaggc tgcaaccatt ccagggtaca agatgtgaac catctaataa 540
ataatgggtt atataatgtc gctcaagagt gtgcctatgc ctttaaagat acgtacgtat 600
taagagtaca ttcacaacgc gagaatgttg aggagccttt tgagattggc agaccaaaag 660
ttggaagtct aggtcgaaac gctgtgataa gcatggaaaa ccccggatac ccactgtttc 720
gcgcaggatt acttcagata acagtaagtg gaacaatacc agctgatata aacgatgaaa 780
tgaatcggtt gaatcagata cggtccgcgt ggattcgaga taaagccact agagaggtaa 840
aagcgatcga gttatgcaca ctgctctcgc ggatagggag gttgaagatg gatatggaag 900
aggaaccaaa agatgaggga gcgatgtctc tgagatttca aggaaagatt gataccatgt 960
tcttctcgga aaatactgag aaaaccaata taatgcgcaa tattagtggg cggacggatg 1020
aagagaggtt ttatgtgttg ttattgatct gcgcaacgga tgcatatacg cggcgtatat 1080
ggagatcgaa cccgtaccca tgtttacgtg gaacgttgat cgctacagaa tgcgtactcg 1140
gagatgcata taaaacgctt cgtcagaagt ttgactggag tgttcgccat gcagcggata 1200
aggcaattga gaatttctcg catattttta caagaattaa cctattcgat acagggaaga 1260
ctccgggagc gcggattata cattgggttc aggagatgac gatgggcacg aaaacgtcat 1320
gggaaagtgg atatcctctg aaggatgaag ctccagatga tgaattacac tgtagagtag 1380
atcgggatca acacagacaa atggttacga gaataattga cggtggatgg gatcatgaaa 1440
attttaagat acataaactc tttcacgaac aaggcaatat atttctaatg gattttgaaa 1500
aggacgcgag acttacatcc agatcggaag ttgaatatcc gtactactac gatcgatgga 1560
tttatgcctc gatcttcgac acccgttata aaataatgga aaccgagata gccacagcga 1620
aaaacgcgga tccagcaata aagcgaacgt tggctccttt gatggatgat ccggttatgc 1680
ttcaattacg aacgatcgga aacttatatg acactcgccc tgccatcatg ggctctacgt 1740
tatcagcgcg gcaggcacag acatccctac ataagaccct cttggacgag gcggagaatg 1800
gaagatatta tagatcgaga ggtctcgaga agcgtgccaa tccgtgctcg gtttgttaca 1860
cgtcatcctt cgtcttacag aaggtatccg agctagtctt gagtctgatg atttatcacg 1920
ttgaccatga ttgggagccg cttaagcaat acaaccatcc agatgttctt tcagaattct 1980
ccgaaaccgt tcgggaaatt catgatatat ctcagctgat tgttttatta gttgatttat 2040
tctttgagca gaggcgaacc gttcgcccag ttgaagaggc aaggtacgtc atttattcaa 2100
tacgcgtggc gaggggtgct gatagacttg acacattccg gagattcttt cccgcgtttg 2160
gaagggcttt agacgtagta aacagggcaa cggacgtcgc gcatatcacc gcgcttaatt 2220
ttctgccttt tttcttcctc ttaggggata atataatata taagcataga gagtggacgg 2280
ttcctgtcct catctatacg gagggagtca tgatttggcc ggcgcaggtt ggtgctaatt 2340
tcaataggtt tggattctgt ggatttttga attacatgcg atttcacgca ggcagccgtt 2400
taagagcgaa gccacttgat gatgctgaaa agatagtcac acgcgaaatg ttgcgttatt 2460
atatgaagac tgacatcttt gatggcgggg tacaaaagaa tatacgagta acgaaatcat 2520
ctcagatgga gatctatcat tcgtcactat gtggaggact ttcagatgcg gtcgtttatg 2580
tattgccaat cagttttccg attaagtgtt taactttgat tatagttggt gatgacctgg 2640
tcgagccgca gatgaggttt ggaagggtga tggatttctt taggcatgtt gcggaacatg 2700
tgcgcggagt cgcttcgata tcaatctcac gaaatggtga cattacaaca cattcgcaag 2760
gtattgttag ggttgaatta ttgaagaaaa atatccttaa gtatcagttt caggtcgctt 2820
tgcttaaagt aaaggggttc gttttcggca acgatgagat cttaattaaa ctactgaacg 2880
tatagtctca gtgacatggg actcctgaag cttacactta c 2921
<210> 98
<211> 1635
<212> DNA
<213> 人工序列
<220>
<223> BTV9 AJ586708的VP5 DNA
<400> 98
gttaaaaagc gtacccttag cgaagatggg taaagtcata cgatccctaa gtcggtttgg 60
taagaaagtt ggaaatgcgc taacgtcgaa tacagcaaaa aagatttaca gcacgattgg 120
aaaggcggct gaacggtttg cagaaagtga aatcgggtcc gcagcgatag atggcttagt 180
gcaagggagc gttcattcaa taataactgg tgaatcatat ggggagtcag taaagcaagc 240
ggtgttatta aatgttttag gaagtggtga ggaaattcct gacccattga gcccaggcga 300
acgtggaatt caaacgaagt taagagaatt agaggatgag cagagaaacg agttggttcg 360
tcttaaatat aatgatgaaa ttaaaaggaa atttggggaa gagttagaac aagtttacga 420
ctttatgaat ggggttgcga aggacgagga gggcgaagag aaacagtttg atatattgaa 480
caaggcagtg aactcatatg acaaaatcct agcggaggaa gatacgcaaa tgaggcgttt 540
ggcgacagct ctacagaaag agattggaga tagaacgcac acggagacga cgatggtgaa 600
ggaatataga agtaagatcg aggctttgaa gaacgcgatt gaagttgagc gagatgggat 660
gcaagaagaa gcaatacagg agattgcggg tatgaccgct gatgtgttag aggctgcttc 720
agaggaggtt ccattgattg gagcaggaat ggcgacagcc gtagctacgg ggagagcgat 780
tgagggagct tataagctca aaaaagttat aaatgcgctg agcggaattg atttgactca 840
tctacgcact ccgaagatcg aaccgagcgt tgtatctacc atacttgagt atcgtgcaag 900
ggatgtaccg gataaagcgt tggctgtaag tgttttatcg aaaaatcgga caattcaaga 960
gaaccacaaa gaactaatac atataaagga cgaaatcttg ccaagattca aaaaagcgat 1020
ggaagaagag aaggagattt gtgggataga ggataaaacg atccatccaa aagtcatgat 1080
gaaatttaaa atacctagaa cgcaacaacc ccagatacac atatatagtg cgccgtggga 1140
ttcagatgat gtcttctttt tccattgtat ctcacatcat catgcgaatg aatctttctt 1200
tcttggattt gatttaagta ttgatctagt tcactatgag gacctgaccg cgcattggca 1260
cgctttaggg gcagctcaaa cagcggcagg acgtacgctt acggaggcgt ataaagaatt 1320
cttaaacttg gcaatttcaa ataattacgg tactcagatg cacacgcgga ggatgataag 1380
atctaagacg gtgcacccca tttatttggg atcgctccat tacgatgtgt ctttcgcaga 1440
cttacgtgga aacgcgcagc gtatagtata cgatgatgaa ttgcagatgc acatattacg 1500
gggaccacta cactttcaaa ggcgagcgat cctgggtgcg ttaaagtttg gatgtaagat 1560
cttaggggat caaatagaca taccactctt cttaagaaat gcttgaacgc agcgacggga 1620
agcgcttaca cttac 1635
<210> 99
<211> 2926
<212> DNA
<213> 人工序列
<220>
<223> BTV10 AJ585131的VP2 DNA
<400> 99
gttaaaagag tgttctacca tggaggagtt cgtcatacca gtgttctctg agagagatat 60
cccatattca ctgctgaacc actacccatt ggctatacgg atagatgtta aagttgatga 120
cgaagatgga agacataacc ttattaagat acctgaatca gatatgatcg acgtaccaaa 180
gttgagtgtc atagaagctc taaattatag gccaaaaagg aatgatggag tggttgtgcc 240
gagattgcta gatataacac tacacgctta tgacaagagg aaatccacga aaagcgcgaa 300
aggggttgaa tttacaacag atgctaagtg gatgaaatgg gcaatcgatg ataagatgga 360
tatacaacct ctcaaggtta ctttggataa tcactattcc gtaaaccatc agcttttcaa 420
ttgtatagtt aaagcgaggt cagcaaatgc ggacaccatc tattacgact attatccatt 480
ggaaaatgga gcaaagaaat gtaaccatac aaacctggat ttattacgaa gtttaacaac 540
gacagagatg ttccacatct tacagggagc ggcttatgcc ttaaaaacaa cgcatgaact 600
agtagctcat tccgaaaggg aaagcacgag cgagacttat caggttggga cgcagagatg 660
gatacagctg cgtaaaggta caaagattgg gtatagaggg cagccgtacg aacgattcat 720
atccagctta gttcaagtaa tcattaaagg aagagttccg gatgagatta gagacgaaat 780
cgctgaactt aatagaatta aagatgaatg gaaaaacgcc gcgtatgata gaactaaaat 840
acgcgcattg gagctctgca aaatactctc ggcgatcgga cgtaaaatgt tagatgttca 900
agaagagcca aaggatgaga tggcactatc cactagattt cagttcaaat tagacgagaa 960
attcattaga accgatcaag agcacgtaaa catatttgaa gtaggtggtc ccgcaacaga 1020
tgatgggaga ttttacgcgc tgatcgctat agcggctacc gatacacaac aaggtcgcgt 1080
gtggaggacg aatccttatc cgtgtctacg cggtgcgcta attgctgctg agtgtgagct 1140
tggcgacgtg tacttcacat tgcgccaaac atataagtgg agtttgcgtt cagaatatgg 1200
tcaacgtgag aggccgctag aagataataa atatgtattc tctcgtctta atctgtttga 1260
cactaaccta gctattggag atgaaatcat tcactggcgg tatgaaattt atcggcctaa 1320
agaaacaact catgatgatg gatatatttg tgtatcacag aaggatgatg acgaattatt 1380
atgtgaaatt gacggggata ggtataaaga gatgtttgat cgtatgattc aaggcggatg 1440
ggatcaggag cgatttaaac tacacaatat attaactgaa ccaaacctgc ttactatcga 1500
cttcgagaag gatgcctatc ttggttcgcg ctctgaatta gtatttccac cttattatga 1560
caaatggatc aactcgccga tgtttaatgc caagcttaag attgcgcgtg gcgagatcgc 1620
aacccggaag gtggatgacc cttggaacaa tcgagcggtt catgggtaca ttaaaacgtc 1680
agcggagtct ctagggtatg tgttagggcc atattatgac ctacgtctgc aattattcgg 1740
cgacgctctc agtctggaac aaaaacagtc agccgtattc gaatatatgg ctcaacaaga 1800
tgatttcccc gcgctaacag attatacgaa ggagaagaac ggttgccctc attcaggcgg 1860
aaccttttac actttccgca aggtagcgtt gatcatttta tcgagttatg aacgattgga 1920
cccaagttta catgagggga gggagcatga gacatacatg cacccggcaa tcaatgatgt 1980
tttcagacga tacgctttag aaatgaaaga attttctcaa ctgatttgtt ttgtgttcga 2040
ctatattttc gagaagcatg tacagctacg caacgccaaa gaggcgagaa gaattatata 2100
tttgatacaa aacacgtccg gtgtgcaccg attggatatt ttacgcgaaa ctttccccaa 2160
ctttcttagg cacgttatga atttacgcga tgtcaaacgc atatgcgatc tgaatgtgat 2220
taatttcttt ccattgttat ttctgattca ggataatatt tcgtattggc atagacagtg 2280
gtcgatccca atgattttgt ttggtgaagt cgtcaggcta atccctatcg aagttggggc 2340
gtatgcaaat agatttggat ttaagagctt cctcaatttc attaggtttc accccggcga 2400
ctcaaagaag aagcaggatg cggatgatac ccataaagag tttggctcaa tatgttttga 2460
gtattatact acgacaaaaa tttctcaagg tgagatagat gttccggtca tcacatctaa 2520
attagacaca cttaagttgc atatagcctc tttatgtgcg ggactagctg attctcttgt 2580
gtatacgctg cctgtagcgc atcctaagaa aagtatcgtt ctcattattg taggggatga 2640
taagcttgag ccacaagtac gctcagagca aatcgtaaat aagtatcatt attcgcggag 2700
gcatatctca ggggtcgttt cgatctgcgt ggaccagaac ggccaactga aggtacattc 2760
tatggggatt acgagacatc gaatatgtga taaatcgata ttgaaatata aatgtaaagt 2820
ggtattggtt aggatgcccg ggcatgtctt tggaaatgac gagctaatga cgaagctact 2880
aaatgtttag gtcctgtgac acggaccggt agcctcttac acttac 2926
<210> 100
<211> 1638
<212> DNA
<213> 人工序列
<220>
<223> BTV10 AJ586709的VP5 DNA
<400> 100
gttaaaaagt gttctcctac tcgcagaaga tggggaagat aatcaaatcg cttagtcgtt 60
ttgggaaaaa ggttggaaat gctttaacat cgaatacagt gaaaaagatt tattcaacga 120
ttggtaaggc ggcggagcgt tttgcggaga gtgagatagg tgcggcgacg attgatggat 180
tagtgcaggg aagcgttcac tcgataataa cgggggaatc atatggggaa tcggtgaaac 240
aggcggtcct actgaatgta ttgggtacgg gcgaagagct gcctgatccc ctcagcccag 300
gtgagcgcgg catgcagatg aagataaaag agctagaaga tgagcaacgt aacgaacttg 360
tgaggcttaa atataataaa gagattaccg agaaatttgg caaagagctt ggagaggtgt 420
atgacttcat gaacggcgag gcaaaagaag tggaagctgt tgaagaacag tatactatgc 480
tatgcaaggc cgtcgattct tatgagaaaa ttctgaagga agaggactca aagatggcta 540
ttttggcacg agcgttacag cgagaagcag cagaaagaag tgaagatgaa atcaaaatgg 600
tgaaagagta tagacaaaag attgatgcac tcaagagcgc gatagagatt gagcgtgacg 660
ggatgcagga ggaagctata caagagatcg ctggaatgac agcggacgtg ttagaagcag 720
catcagaaga agtgccattg ataggtgctg gcatggcaac ggccgtcgcg acggggcggg 780
cgatagaagg agcgtataaa ttaaagaaag tcatcaatgc actcagcgga attgatcttt 840
cacatatgcg aagccctaag attgagccta ccattatcgc cacaactctc gaacataggt 900
ttaaagatat acctgatgaa cagctggctg ttagcgtatt aaataagaag acggctgtag 960
ctgataattg cagcgagatc gctcatatta agcaagagat attacccaag tttaaacaaa 1020
taatgaatga agagaaggaa atagagggca tagaggataa agttatacac ccgagagtaa 1080
tgatgaggtt taagatccca cgtacgcaac agcctcaaat tcatatctat gcggctccat 1140
gggattcaga cgatgtcttc tttttccact gtgtgtccca tcatcatcgg aatgaatcat 1200
tcttcctcgg ctttgatctg ggaattgatg tagttcactt tgaggacttg accagccact 1260
ggcacgcgtt aggaatggca caagaagcta gtggacggac tttaacggag gcctatagag 1320
agttcctcaa tctttcgatt tcgagtacgt ttagcagcgc gatacatgcg aggcgtatga 1380
taaggtcacg cgcggtacac cccatctttc taggctcaat gcattacgat ataacctatg 1440
aagctctgaa gaacaacgcg cagcggatag tttacgatga tgaattgcag atgcatatac 1500
ttagagggcc gttacatttt cagcgtcgag cgatattggg tgcgttaaaa ttcggagtta 1560
aaatcttagg tgataaaata gatgttcccc tcttcttacg gaatgcttga acgcagcggg 1620
ggaggaactt ccacttac 1638
<210> 101
<211> 2926
<212> DNA
<213> 人工序列
<220>
<223> BTV11 AJ585132的VP2 DNA
<400> 101
gttaaaagag tgtcccatca tggaggagtt cgtcataccc gtctattcgg aaacagacat 60
tccgtactcg ttgttaagcc actacccgtt agccattcgt acagatgtta aaatagcaga 120
caccgatgag ggccatgatg tagtgaagat accagaatca gatatgatag acgtcccaag 180
agtagacatt gtggaggccc tcgccgctaa gcctatgagg aatgacggga tcgtggttcc 240
tagactgctt gatattactc ttcgagctta tgacgataga aaagcaataa aaagcgcacg 300
aggtgtagag tttatgacga atgctaaatg gatgaagtgg gcgatcgacg atagaatgga 360
tattcagcca ctgaaagtag ccatagacca ttataatgct gttaaccacc aactttttaa 420
ttgtattgtt aaggctaggt ccgcaaacgc agatacaatt tattatgact attttcctct 480
cgagagtaaa gtgaagaagt gtaatcatgc caacctggac ctgttaagaa gtctgacaaa 540
cactgagatg tttcacatgt tgcagggggc agcttatagt ttaaaatcga attatgaact 600
gatcacaaat tctgagagga atagtaccga agaaacttac gcgcctggag tgcacaatca 660
aattcgctta gtgagaggca cgcgaatcgg gtataagggt gaagcatata gccgatttgt 720
ttctagtcta gtacaagtta ggatacaagg acggacacca cctgagatag ttgacgatat 780
cgcgcggcta aatgtgatca ggacggagtg gatcaacgcg caatttgata gcaccaagat 840
acgagcgttg gaactatgta aaatactttc agcgattggg cgtaagatgt taaacacaca 900
tgaggaacca aaagatgaga tggatttatc cacaaagttt cagttcaaat tagatgataa 960
atttaagaag acagattcgg aacatataaa catatttaac gtcagagcac cggcgacaca 1020
tgagggtagg ttttacgcct taatagcaat tgcggcgacc gatacgcagc gtggtaggat 1080
ttggaggaca aacccgtatc cgtgtctaag aggcgcgctg atagcagcgg aatgtgagct 1140
cggagacgta tatcatactt tgcgccaagt ctacaaatgg agcttgcgac aagattatgg 1200
tcgtacagag gtccctctag agaacaacaa atacgtattc agccgaatta atctgtttga 1260
ttcaaatctg gatgtgggcg atcaagtagt acattggatg tatgaagttg acggcccagc 1320
tgagacaacg tatgacaacg gttatatgtg taagacggag cgggaggatg aagagttagt 1380
gtgcaaaatt agcgaagaga aatataagac aatgctcgat agaatgatcc agggtggttg 1440
ggatcaggaa agattcaagc tttacagtat actgacagat ccaaatttac ttacgataga 1500
ctttgaaaag gatgcgcatc tgaatattag atcagaattc gtgctcccaa gttatttcga 1560
tcaatggatc tactcaccca tgtttaacgc gcggttgcga ataactcatg gagagattgc 1620
gactagaaag tcagcagatc cgtggaataa gcgtgtcgta tttggatata ttaaagcatc 1680
gacagagagt cctgaatacg cccttggaca atatttcgac atgcgtatac aattttatgg 1740
ggatgcgcta agctcgaagc agaatcaatc agcggtcttt cagtatctga gccaacaaga 1800
ggactttcct acgctgacga gctatgccaa aggtgatgtg gtgtgtcctc attcaggtgg 1860
cgcgctgtat acttttcgga gggtggcact gatgctgatg gcgaattacg aaaagttgag 1920
cccggacttg catgaaggta tggaagatta cacttatacg catcccagta tcggtggcgc 1980
ctatcaaaaa cgcattcttg agatgagaga tttttcgcaa ctaatatgtt tcataatcga 2040
ttatatattt gagaggcatg atcaactgcg tgatgcaaag gaggcgagga ggattttgta 2100
tttaattcaa aacttggacg agccgcagcg actagagatg ttaaatgtta cgtttcctaa 2160
cttctttcgt catttcttga aattaaagga tgttcagcta atcagtgatt tgaacgtgat 2220
aaacttctta ccgctgttgt ttctagtgca ggataatata tcctattggc ataggcaatg 2280
ggcggtcccc atgatactat acgatgacgt aatcagacta atccctgtag aggtcggagc 2340
gtatgcgaac agattcggaa taaagagttt cttcaacttt acgagatttc accctggcga 2400
tgcgaaaaaa cgacagaaag ccgacgatac acacaaagag tttggatcga tcagctttaa 2460
ttattacgca agtactaaga ttgcgcaggg aggggtgcat acgccagtag ttacaaccaa 2520
gctagacacg ctgaagattc atctgtcgtc tctttgcgct ggcttagcgg attcgattgt 2580
ttacacgctg ccggtcgcac atcctaaaaa atgcattgtg ctaataatcg taggagatga 2640
taagctagaa ccgcatgtgc ggtcggaaca ggtcgttagt aagtattatt tctcgcgtag 2700
acacgttagc ggaatagtgt ctatatgtgt cggacaggat aatcaactaa aggtatattc 2760
atcaggtatc gtcagacata gagtatgtga gaaatttatc ctgaagtata agtgtaaagt 2820
ggtgttagtt aagatgcccg gatatgtatt cggtaacgat gaattaatga cgaaactatt 2880
aaatgtttag gtcctttgac acggaccgat ggcctcttac acttac 2926
<210> 102
<211> 1638
<212> DNA
<213> 人工序列
<220>
<223> BTV11 AJ586710的VP5 DNA
<400> 102
gttaaaaagt attctcctac tcgcagaaga tggggaagat aattaaatcg ctaagtagat 60
ttggaaagaa agttggaagc gcattgtcgt caaacacagc gaagaaaatt tattcaacta 120
ttggaaaggc agcggagcgt tttgctgaaa gcgagatcgg tgcggcgaca atagatgggt 180
tggtgcaggg tagcgttcat tccataatca caggtgaatc gtatggagag tcagttaagc 240
aggcggttct tctcaacgtg ttaggtacag gtgaagagtt accagaccca ctgagccctg 300
gcgaacgtgg catgcaaacg aaaataaagg agttggaaga cgagcagcgg aatgagcttg 360
tccgattgaa atataataaa gagataacaa aagagtttgg gaaggagcta gaagaggtgt 420
acgacttcat gaatggcgag gcgaaagagg aggaagtggt tcaggaacaa tactcaatgc 480
tatgcaaagc agtagattca tatgagaaaa tattaaaagc agaggactcg aaaatggcaa 540
tattagcacg cgcattgcag cgggaggctt cagaaagaag tcaagatgaa atcaaaatgg 600
tgaaggagta cagacagaaa attgatgcgc ttaaaaatgc gatcgagatt gaacgagacg 660
gaatgcagga ggaggcgatc caggagattg ccggaatgac cgctgacgtc ttagaagcgg 720
catcggagga ggtgccttta atcggcgcag gtatggccac agctgtagcg accggtaggg 780
cgatagaggg cgcatataaa ttgaaaaaag tcataaacgc gttaagtggg attgatttat 840
cgcatatgag gagtccaaag attgaaccca ctattatcgc cacgacattg gagcatcgat 900
ttaaagagat accggacgag cagctagcgg taagtgtttt gaataaaaag acagctgtaa 960
ctgataattg caatgagatc gcgcatatca aacaagaaat attacccaag tttaaacaaa 1020
ttatggatga ggagaaggag attgagggaa tagaggataa agtgattcat ccgcgtgtga 1080
tgatgaggtt caaaattcct agaacgcagc aaccgcaaat ccacatttat gcagccccct 1140
gggattctga tgatgtgttt ttctttcatt gcgtttcaca ccatcatcgg aacgaatctt 1200
ttttcctggg atttgatcta ggaatcgatg tcgttcactt tgaagattta actagtcatt 1260
ggcacgcatt gggattagcg caagaggcga gcgggcgtac gttaacggag gcgtatcgtg 1320
agtttctcaa cttatcaatt tcaagcacat atagcagcgc tatacatgcg aggcgtatga 1380
tcaggtcgcg agcagtacac ccaatttttc taggatcgat gcactacgat attacgtatg 1440
aagctttaaa aaacaacgcg cagagaatag tctatgatga ggagctgcaa atgcatattc 1500
taaggggacc tttgcacttt cagcgtcgag ccatcctggg ggcgttgaaa tttggagtta 1560
aaatattagg cgataaaatt gatgttcccc tcttcttacg aaatgcatga acgcagcgag 1620
ggagaaactt acacttac 1638
<210> 103
<211> 2904
<212> DNA
<213> 人工序列
<220>
<223> BTV12 AJ585133的VP2 DNA
<400> 103
gttaaaagtt gcgaggatgg aggattttcc gatatgcgta attgaacgcg agtccaagtt 60
tgatgaagat ttcgtcgtga gatatccggt gataattgat ttaaaagaat gtgtggatga 120
tgtaaatggg aagacggatg tgtcgaagct ggaggaaact cggggaattt tcgttcgtgc 180
tgagggcata cgagtcgcat taaattcaag accagacaat ttatctcatg ttataacacc 240
gtccgcttta gatgtgttga ttcgggcgta tgatagaaag cacaaaattg cgttagacaa 300
acgtacggat gattctggca gaatggcgga tcacaatata gatgtgtggg ttaagaagcg 360
gttggaagag caagcggatt tacaagccat atatcatgat gttgatttag gacgtggtaa 420
gttaaagttt aaaatgctaa cggggttggt gaagctagat tcgaattatg cggaaacagt 480
tggctacgat tatgcaccgg tgccgaaagt gggatgcgat catgggaatg tcgattttct 540
atatgacttc attttatcgg gtagcctcca ggtatcgaag accgcgggat acactttgaa 600
gcaaacgtat cgattaactg tgcacgctga agctaacgca gaaacacgag atcaactcat 660
cgtaaatggg gtgtataccg agggcgttac gcgcggtgag gagataaggg atatgaagaa 720
aatgtgttat tcaagattcc gtgatggatg gattcgagct actatacagc ccaaaatagt 780
cgaggagttg agacggagta aggaggcgct agacagaata tcgagtgaat ggtatgaaaa 840
gttgacgacg ccagacgttg ttgaaatatg taaaatcgtg tcatcaattg gacgtcagat 900
gtggaatagt gaggaattgc cggtcgatga tgcaatgaga tctaaagttt ttcaggaaga 960
gttgcggctg ttattccgag ttgggaatag cgaatatgat acaattcagg ctatccagag 1020
tggacaaacg cctttgaaga aattctatgc cttgttagcg attgccgcga ctgacagcta 1080
tcgttggcgc atatggtgga gtaatccgta cccctgctta cgaggaacga tcattgcgtg 1140
tgagatggaa ctaggggacg tctacgaaac tttaagatca actttccatt ggagtttacg 1200
tcctacatat gctacccgga aggagattga tcgcgaacga agagcctatc catatcagag 1260
aataaacctc tttgaatcgc gtttagatcc tggaacccgc ataatcaatt ggttggtagt 1320
aagggagccg ttagctgaac cagacgtcaa gtccggtcgt gtgtgtatga cgtcgacaga 1380
atctgaatac attctaaagg tggatgacac gaagtataga gaaatgattg gtcagataat 1440
tgagcggggg tgggagaaag aacatttgaa actggaaaag attgtccagg atgagggtaa 1500
cgtgtttcaa atggaatttg aaaaagatgc gagcttagat gagaggagcc atttagttat 1560
gccgtattat tatgataaag aaatacactg tccgatgtat cataagaaga ttaaaataac 1620
agaggctgag attgcaactt catggagtga tgacccatgg ttaaatcgcg tgttaaatgg 1680
atttttaggc gaccatgtgg agacgtttaa tttagggtgc aaccacatat tcgacatgcg 1740
agtaccttta aaaggaaatt tactgacaca agagcaggct atctcaagtt accacgaaca 1800
cattaggtcg ccggaagcac agagcgagaa ccaatgtgca ctgaaggtat atgcggatga 1860
ggcggtcagt aagctgttta ctactttgta tcacagacta gcctatcatc ttccaaagac 1920
ggagtacata gagcgagaag aggaggtgag tcagtatgag ggattagtaa agagaattcg 1980
tacatgggac cagttcatta tattcgtatt tgatttcttc tttgaaaata gaaaagggtt 2040
acgcacgacg gaggaggcga cacagcttgc taaggcaata aggagtaatg ttggcgcgac 2100
gcgatggacg atgatggcga gtgcgtttcc taccagcgtc agattagtga agggaatcct 2160
tcgagcgaag acgtggggtg aatgttatat aatgaatttc ttaccattgt taatatgtgg 2220
gggctcatcg atcagctatt tacacagaga atggagttat catatgatcg tagtctttga 2280
ggatggtata aaggtggttc cagctatgac cggacgctca cttacacaag gttcgctggg 2340
agagtgggca tcttttgtgt gcttctttat gggttcgaat cacgaaaaga taaaactcga 2400
agaagacgaa cttgaggttt tgcgaaagtg cgttgagttt tacgaaggtg taaaaatggt 2460
tgatatcatc aaagatactc cggttcgaat gactaagtat attgcatgtg atttagcgtt 2520
tggtagcgga tgtggtggaa caagtgaatc acttagcttc atactgccag tcacacatcc 2580
taacagaagc ttgattattt ttgtggtggc ggacgataag ataagcccag cgcagcatgc 2640
cgcgagagca aaacgtagat tccgtgggac gcgcgaatac gtaaatgatg taatcgtatt 2700
acagatgaaa cgacctggca cagtcggcaa cgtttggagt gagaaacata gtaaagtgag 2760
aatatgtcga aggaattttc taaaatatga tcacaaagtg gttcttacga agttcgcagg 2820
gttagtctat gggaattacg aactaattac gaaactcgca aatctatgag cccttgacaa 2880
ggctccctcg cggcttcaac ttac 2904
<210> 104
<211> 1645
<212> DNA
<213> 人工序列
<220>
<223> BTV 12 AJ586711的VP5 DNA
<400> 104
gttaaaaagc agccaccaaa catcgcatga tggggaagat cattaaatcg ctggggaagt 60
tcgggaaaaa ggtaggcaat gcgttgactt cgaagacggc gcaaaagatt tacaggacga 120
tcggaagtgc ggctgaaagg tttgctgaga gtgagattgg cagcgcagcc atcgatggat 180
taattcaggg tagtgttcac tcaataatga caggtgattc gtatggtgaa agtgtgaagc 240
aagcggttat tctaaatgtc ttaggagggg gagatacgat tgcggaccca ctaagccccg 300
gagagaatga gatatatagg aaactgaaac aacttgagga tgagcagaag ggggagatag 360
ttaggacaaa acataataca gaaattctga agaagttcgg aaacgatttg gatgaagtgt 420
acaaatttgc aacacaagaa cataaagttg gcgagcatga agaagatcaa ataaaaatgc 480
tggagaaggc gctaaaatca tatattgggg ttttcaaggc tgaaaacgat agcattgaca 540
aattgaagaa agcgttgtat gtcgaatcga cggagcggac ggaagaagaa actaagatga 600
taagtgaata ccgtgataaa attgatgcgt tgcgaagtgc aattgaggtt gagaaggaag 660
ggttgcaaga agaagcaatt caggaaattg caggtatgag cgcagaagta ttagaagcgg 720
cggcagaaga ggttccgtta tttggggcgg gcgtagctac gagcatcgcg acggcgaggg 780
cgatagaagg cgcatataag attaaggctg taattagtgc attaaccggc atagatttaa 840
ctcatcttaa cgtgcctaaa gttcaaccta agacgctaga agcgatcctc gatgtaccaa 900
aaggtgtaga tattccagat ctgcgtttaa cggagggact catcgcgaaa atggataaaa 960
tttcagagaa tatatctgaa gttgatcaca tacagaacgt catcgttccg aagctaaaga 1020
aagcggttga ggaggataaa gaaaacatta cggattggtc tgaaaagcgc attcatccga 1080
aaacagcgca acggtttaag ataccacaga cgcagaagcc ttcgatgcac atatatgcgg 1140
ctccctggga ctcggatgaa gttttcatct ttcatgtaat ttcacctcat cataagaatg 1200
aatcattctt cttgggattc gatcttgaga ttgaatatgt tttttatgaa gatttgactc 1260
aacattggca cgcattaggg acagctcaat ctacctatgg cagaactttc cgtgaggcgt 1320
ataaggagtt cttcttactt gcctctaacg tcgcaggaca aacagcaatc catcagcgta 1380
ggttgctaag atccagaagt aaccatccga tttatttagg aatgcatgat tatgaagttt 1440
cgtacgctca aatgaagatg aacgcgatgg cgttggttag caatgaggat ttgcagatgc 1500
acatattgcg tggacccata cactttcaaa gacgtacaat cgttgcggca ttgaaatatg 1560
ggattaagat tttaacccag cctgatgacg tctccatcat gcttcgaaac gcgtgagggc 1620
ggatgcaggg gctacttaca cttac 1645
<210> 105
<211> 2935
<212> DNA
<213> 人工序列
<220>
<223> BTV13 AJ585134的VP2 DNA
<400> 105
gttaaaaacg ctagcccgag gatggaagag ctcgtgatac ctgtgataac cgaacgattc 60
gataagagat tagtagggcg ttatgattat gtgattgagt tggcgaggcc agaaggcgat 120
gaatggagcg gtcacgatgt tacgcatatc ccagataagc gaatgtttga cattaaggtt 180
caaccaataa gagatgcaat tgattacaag ccagttgaaa atgatggaga ggttttacct 240
cgtattttgg atatgtcaat cgcatgctat gacatgagga aaagaatgat gaaaagagat 300
ggcgtagatt ttgtttcaaa cactaaatgg ctagagtgga tgatccaaga ttcaatggat 360
gtgcagcctt tgcgggttga tatgaaagaa gaccatagta cggtacaata cgacatgttt 420
tcagcaaaac tgcatgtcga ttcgaggaaa gcggatacca cgtcatacaa tatacttgct 480
ctcgaggtga agaaaggagc acagtgccat catgtgcatg ctaacatttg gaaccatatg 540
atacgtaatc atttgtttca cgcggtgcaa gaatcatgct atatctttaa gcctacttac 600
aaattgatcg ttaactctga gcggaggacc cctgatgagg attttcagat tggtaatcct 660
cagtttccaa cgctaaggcg caaccagcag atatttttag gtgatgacgc gtataagaaa 720
actgcgagag gattggttca ggtcttagtc aatggtgtcg tgccggacat aataagaaat 780
gagatagcgg cactggatgc aatacgtgac agatggatac agggaaatta tgagcgcact 840
cacataaaat cactcgaact gtgtaatcta ttatccgcca ttggtagaaa aatggttaat 900
cttgaagagg aaccgaaaga tgagagaaat ttatccctca gatttcaaca taaacttgat 960
gataaatttg cgaagaatga tcaagagagg aatgtcatat ttgcgcaaaa gagtcagcgt 1020
aatgatcagg atcggttcta cgtgctgatg gtgatagctg cttcagacac aaacaatagt 1080
agggtttggt ggtctaatcc atacccttgt ttacgaggtg cgctaatcgc ggctgagtgc 1140
aaattaggtg acgtctatta taaattgcga tcatggtatg agtggagcgt acgtgaggga 1200
tataagccac gagatctgga tcgccagtat gaaaaatata ttgtgggacg tgtgaatctg 1260
tttgatttgg aggcggaacc tggaacaaaa gtgttgcatt gggagtatga gcttatctca 1320
aaattatata tcgttagcaa tcatgagggt aaccagtgcg atttgtaccc agatgagggt 1380
gaaatcgtgg ccaagttcga cgatgcacgg tatagtgata tgattcaaac gataataaac 1440
gaaggatgga agcaaggcga ttttaaaatg tttaaagtgt tgaaggatga aggtaaccca 1500
ttactatacg atttagagaa ggacattaag ttggatcgcg tttcgcgagt agtattccca 1560
ccgtattttg atcaatggac ctacgttccc atgtttaacg ccaggataaa gccgtgtgag 1620
gtggagctgg ctgaacgtaa gaacaccgat ccctatgtaa agcggacgct taagcctttg 1680
aaagcggatt gcatcgagtt aatgcgctac catatgtctc agtacatgga tttgagagta 1740
tcgttgcaag ggacgagttt gagcattaag caagtcccct cgagcataca ccaatcgctc 1800
gctcaagatg catcttacgc gggaattctc agccgacgta gagaaaactt agactataaa 1860
tcccaatgcc cgatcgtaac taaccttttc ttattggaga aattctttct actgattttc 1920
actacgatgg agaagcacta ttgggaaatg gatgatgatg aaacacagta tgagcatcct 1980
aagatagatc catcaaagtt tgaggtggag ggcacactac atgatgtttc gcaggtcatg 2040
gttcatctac tcgataggtt ctttgagaag aggcgtttcc tgaggacggt tgatgagtct 2100
agatggattt tacacctgat ccgatccacg agtggctcca aaaggctcga agtgttatcg 2160
agattttttc ctgcgtttag tgacggatta cgcattcgtg aatttaagaa agtaagggac 2220
ataatgttat taaatttctt gcctttctta tttctaacag gtgacaacat ggcttatgaa 2280
cataggcaat gggcggttcc agttattttc tatgcagata aaatcaggat tgtcccggcg 2340
gaggtaggcg catactataa tcggttcggt ttaacgtgca ttctggaatt gatgatgttc 2400
tttccttcct acgatactcg aaatgaggat ctcggtgaag atgtacgcgc atgtataggc 2460
ccaatcatca attactatct agatactacg atttcgaatg gtggcattca gacaagtatt 2520
gtttctacga aagcgttgct ttatgaaacg tatttgtcct ccatatgtgg tggcttttca 2580
gaagcgatcc tatggtattt accagtcact catccttcta aatgcttgat cgcgcttgag 2640
gtttcagatg cactaacgag tcccgaacta cgtattgaca gaattagacg aaggtttcct 2700
ttatcgagta accatttaaa agggattgta caagtttcag ttcgacctga tcgtacgttt 2760
agtgttatca cacaaggcat tgttaagcat agagtttgca aaaagacgct gttgagacat 2820
aggtgtgatg tgatcttaat acagactccg ggctacgtct tcggtaacga tgaattactt 2880
acgaaactct taaatatata atcccgtgac tgggattggg cacgtttaca cttac 2935
<210> 106
<211> 1637
<212> DNA
<213> 人工序列
<220>
<223> BTV13 AJ586713的VP5 DNA
<400> 106
gttaaaaagt tcctcaagat cgcggaagat ggggaagatc ataaaatcac ttagccgttt 60
tggcaaaaaa gtgggtaatg cattgacctc gaatacagct aaaaagattt ataacacaat 120
cgggaaggcg gcggaacgtt ttgctgagag tgagatcggt tcggccgcga ttgatgggct 180
ggttcaagga agcgttcatt caatcatgac gggggaatcc tatggtgaat cagttaagca 240
ggcggtgctg ttgaatgtgc ttggtgcagg agatgaaatc ccggatccac tcagtcctgg 300
agagcgtggt atccagatga aaatcaagga gatcgaggaa gagcagagga acgagttagt 360
gagaataaaa tacggaaaag agataagaga gaaatttggt gagcaattgg aagaaatata 420
ccaattcatg aacggcgaag ttaagggaga agaagagcaa gaggagcaat ataaagtctt 480
atgtaaggcc gttgactcat atgaaaacct actcgtcaca gagaatgagc agatgcgtac 540
gttagcgcgc gcgttacagc gggaagcgac tgaacgaaca gaaaacgaat cgaagatggt 600
caaggaatat aggcagaaaa tagacgcctt aaaggctgca atagaggtgg aaagggatgg 660
tatgcaagag gaggcaatcc aagagatcgc gggcatgacg gcggacgttt tggaagcagc 720
atcggaagaa gtccctcttg taggatcggg aatggcgacg gctattgcaa caggtagagc 780
gattgagggt gcgtacaaac ttaagaaagt gataaacgct ttaagtggca ttgacttatc 840
gcatttacgt actccaaaga ttgagccaac catggttgcg acaacgttag agcatagatt 900
caaagaaata ccggataaag aactagctgt gagcgtctta gcgaaaaacg atgcgatagt 960
agcgaatact aaggagatta aacacataaa agaggagatt ctaccaaaat ttaagaagat 1020
catggaagaa gagaaagaat tggagggtat agacgacaag aagatacatc cgagggtgat 1080
gatgagattt aaggtaccta gatcacagca gccgcaaatt cacatataca gtgcgccgtg 1140
ggactcagac gatgtatttt tctttcactg tgtgtcgcac ttccatgcga atgaatcatt 1200
ctttctgggc ttcgatttgg gaattgatgt tgttcatttc gaggatctcg ctgcgcactg 1260
gcatgcgtta ggcgcagctc aagaagcaaa gggacgtaca ctaaatgagg cgtacagaga 1320
atttcttaat ctgtccattg gaagcacgtt cacatcgcca atgcatgcac ggcgtatgat 1380
taggtcgaaa actgttcacc ccatttattt aggctcgatg cattacgata ttacttatga 1440
aacgttaaag acgaacgcgc agaagcttgt atatgacgaa gatttacaaa tgcacatatt 1500
aagggggccc ttacactttc agcgacgcgc tatattaggt gcgttgaagt ttggtgtaaa 1560
agtgttaggt gataaaattg atgttccgct cttcctaaaa aacgcgtgac agcggtgatc 1620
ccggggctta cacttac 1637
<210> 107
<211> 2922
<212> DNA
<213> 人工序列
<220>
<223> BTV14 AJ585135的VP2 DNA
<400> 107
gttaaattag tttcgtgatg gatgagctag cgatcccaat tataaatgga cacttcccaa 60
aagagacgtt aatagagtat gattgtatca ttgagttaaa tccgccagtt gaagatgatg 120
cagccgatgt ggttcagatc ccaacaaggg acatgatgga tattccgaat gtgccgatac 180
gtgatgcctt gaaacaccta ccacagagaa atgatggtca cgtcttgacc cgagtacttg 240
acatctcttt aagcgggtat gaccggagga aatatcacaa gacgaatagt ggaggggaat 300
tctattcacc gaacgagtgg cttaactgga tgatcaatga ctcaatggat gtgcagccac 360
tgaaggttag cttaggtttg ccaccaacta ttagacatga attgtttagc tcgagcgtgt 420
ttatatccgc gaaaaaggct gacaccacat cataccatat tgaaccaata tgtgagaaag 480
acaaagcatg cgatcatacg cgcgtttcca tgtggaataa tctaacgagg catgaaatga 540
tgcactgcgc gcaagagagt gcatatgtgt taaaaccaac ttacgatata actgtttatt 600
cagaaagagc cacaactgat gaggcgttcc agcaggtggg gcagaagttc gttactataa 660
ccaaaaacca tcgagtggta ctgggtgatg atgcttataa gaaaacgttg ggcggattaa 720
ctaggttgcg tgtgcagggg aaaataccat ctctgataca aaatgatata cagcagttga 780
accaaattag ggatacgtgg attagagatt catataatcc gaggaatata cgatcgctag 840
aattgtgtaa attgctgtcg ggtataggta ggaagatggt ttgtatagaa gaggaaccga 900
agaatgagag tgacttatca gtgaaatttc aatttaaact cgacgagaag tttcgacttg 960
atgatagtga acgtggggtc atcttcacaa ataaaggtca gagaaatgat caagatcgtt 1020
tttacgttct aatcatgata gcagcatcgg acacgtataa cagccgtatc tggtggacga 1080
atccgtaccc ttgtttacgc ggcgcactga ttgcggcgga gacacaatta ggtgatgtgt 1140
attggacctt aaggcatttt tacgaatgga gtgtaagacc agcatattcc ccgaggttga 1200
gggagcgtga ggatgacaaa tatatatatg gcagagtaaa cttattcgat ttagatgcga 1260
gtcctggtac aaagataata cattggacat acgatctgat tacgcgggag agtaagatta 1320
catatcataa cggaaatcca tgtgatttga acccggatgt ggacggcatc atcacaaagt 1380
ttaacaaaac tgcctataca gatatgatcg gtgagttgct taatggcggg tggaacaccc 1440
gtgaattcaa gatgtataaa atattagaga gtgtagggaa tgttctaacc attgactttg 1500
aaaaggatgc gaaactcaac tctcgatccg agtttatttt gcctgactat tacgacaaat 1560
ggatatatgc gcctatgttc aatgcgaagc ttaagataac agaagttgaa attgcgcagg 1620
ccaaaagtga cgatccggac gtagtgagaa ccttagcacc tattggctca gatccgatta 1680
ctttgcaacg gttgtgctta ggaaattatt atgatatcag acccgcaatg catggacgcg 1740
cattgatgaa gaaacaggag cagacgtcct atcataagac cttggctaaa acgaaggatt 1800
acagtgatgt acttaatcga agagggtatc gtaaagttga gaagccatgt ccaatggtaa 1860
cgtgctcgta cgtattggag aaaacggctt tattttggat cgacgttatg gagaagcatg 1920
ttggggacat tgaagacagt acggatgagt tcgacttccc aagggtggat gtaactttac 1980
cggtggagac tcacaaagtt gtagatgtcg ctcaactgtc agtgcttctt atagactttc 2040
tgtatgaaaa acgacgtaca gtgcgagata tcgatagcgc tagatggacg atcgaattga 2100
tcagacgagt gagggggaag gatcgacttg aagcgattcg tcgggagttt ccaaactttg 2160
gtgaagcggt tacgattctt atgaatccta cgagagttaa agatgttatg gttattaact 2220
ttctcccatt tcttatcttg ttgggtgata acatctcgta cgaacacaga caatggtcca 2280
ttccgataat tttacatgcg gattcaatgt ggtttctccc ggtggaggta ggggcgttct 2340
ataatcgatt tggtgtcata tcgttcttag agtatatgat gttttttcca tctgcacagt 2400
cgagggagtc tgagctggat gaagcgaaga ttaaggtatc aaagttaatc ttagaattct 2460
atctagatac aaccattttt tcaggtggcg tacaacgaag cgtcgtttct accaagcaga 2520
tactttacga aacttatcta tcatcactat gcggtgggta ctccgagggg ttggtttggt 2580
atttaccgat cactcatccg acgaagtgcg tcgtcgccat tgaattttct gatgataggg 2640
ttgatgcagc tcacaggtgt aaccggcttc gcgcgagatt tcccttaagc gcagtgcatc 2700
tgaaaggaat agtgattctt tcgatagcta aagatagagc ggttaaggcg tatactgaag 2760
gtatagttac gcatcgttta tgcaagaaaa atgtgcttgg gttcacttgc caaattctac 2820
tgttaaagtt ctcaggacat gtatttggga atgatgaaat gctaacaaag ctgcttaacg 2880
tttaacctca gtgacatgag gtgcacgaac taatcaactt ac 2922
<210> 108
<211> 1637
<212> DNA
<213> 人工序列
<220>
<223> BTV14 AJ586714的VP5 DNA
<400> 108
gttaaaaagt tccccatgat cgcgaaagat gggcaaaata atcaagtcgc tgagccgttt 60
tggcaaaaaa gtaggcaatg ctttgacatc aaatacagca aagaagattt acagtacgat 120
tggaaaggca gcggagcggt tcgctgagag tgaaatcggg tcggctgcaa ttgatgggtt 180
agtccagggt agcgttcact caattatgac gggcgaatcc tatggagagt cggtcaagca 240
agcggtgctg ctgaacgtgt taggggcggg ggatgagatt ccggatccgt taagtccagg 300
tgagcgtggc atccagatga agataaaaga gttggaagaa gaacagagaa atgagttagt 360
taggttgaag cacgggaaag agatcacgaa gaagtttggc gaggagctag aagaaatata 420
tcaattcatg aatggggagg ttaaggatga ggatgagcag aaaaaacagt atgagatgct 480
ttgcaaggct gtcgattcat atgagaagtt actcgtggcg gagaatgacg agatgcgcac 540
gttggctcgc gctctgcgac gggaaacgac tgaacgaacg gaggttgaat ctaagatggt 600
gaaagaatat agacaaaaga tagacgccct aaaagcggcg attgaggtac aaagagatgg 660
gatgcaggag gagggaattc aagagatcgc gggtatgaca gctgatgtgt tagaagccgc 720
ctcagaggaa gtaccattag tgggatcggg gatggcaacc gccatagcga ccggacgagc 780
gatagagggc gcgtataaat taaagaaagt gataaacgcg ttaagcggta tcgatctttc 840
acatctacga acaccgaaaa tagagcccac catgttcgcg accaccttag aacacagatt 900
tagagatata cctgataagg agctagcagt cagcattcta gcgaagaaca acgcaattgt 960
ggcgaatacg agggaagttc agcacataaa agaagaaatt ctgcctaaat tcaagaagat 1020
aatggacgaa gagaaagagt tggaaggtat tgatgataag agaatacacc caagggttat 1080
gatgaggttc aaagtgccac gatcgcagca acctcagatc catatctaca gcgcgccgtg 1140
ggattcagat gatgtgtttt tctttcattg cgtttcgcat tttcatatga atgaatcttt 1200
ctttttaggt tttgatctgg ggattgatgt tgtccacttt gaggatctag ccgctcactg 1260
gcacgcgctc ggggctgctc aggaagcaaa gggacgtact ctaaacgagg cgtacaagga 1320
atttcttaac ctatcaattg gcagcaccta tacatcacca atgcatgcga gacgtatgat 1380
tagatctaaa actgtccacc ctatctactt aggctcgatg cactatgata tcacttacga 1440
gacgttaaaa tcaaacgcac aaaggatcgt ttatgatgat gaacttcaga tgcatatact 1500
gagagggccg ttacactttc aacggcgcgc aatactagga gcgcttaaat tcggagtaaa 1560
agtgctaggc gacaagattg atgtgccact ctttctaaaa aacgcgtgac tgcggtgatc 1620
ccgggacttc tacttac 1637
<210> 109
<211> 2909
<212> DNA
<213> 人工序列
<220>
<223> BTV15 AJ585136的VP2 DNA
<400> 109
gttaaaagtt gccgggatgg aggatttccc tgtgagcgtg atcgaacgta attcaaccat 60
cgcaccagcg attataaaga gatatcccat catagtcgat gttaggaata tggtcgcttt 120
atcgggaggg aagaatgatg ttaccaacat accacgagtg cgaaacatag gtgtaagaca 180
agaagggata cgtgttggac tgaaatatcg tccagatact gagacgaatg ttatctttcc 240
taaaaattta gacgttctaa taagagcata cgatcataaa catcgattga cgagggataa 300
gagaccgact gaccatgacg tggactctat ttcagacggc gacgttacgg tgtgggtaac 360
gaatcgaacg aatgagttct atgatataca accaatttac cacacgttgg aggacgaagt 420
ctgcaaagta aaattcacct cgctacttgg gagcgtttca accgattcaa attatgcgga 480
aagtatgtct tatcattata tcggtgttga acgcgataag tgcgatcact caaagtggca 540
tagatttttt cccatgcttt tgtcagattc gattaactta ggcaaggagg taggttacat 600
attgaaaaaa acgtataaaa ttaaggctat aggagaggct cgggagggac aaagacagga 660
gttgcgagtg gatgtcccat atacccctgg cataggaaga aacgagagga tcagcgacat 720
gaagttgccc gtatataagc gtttcctcga aggctacata cgctgtcaaa tcgagccaaa 780
cgtccccgag aagttaaccg agctgaagaa tcagcttgat gcgatctgtg tggcgtggta 840
tggtggacag gtgcccatag tcgctgatga aatctgcaaa attacctcgc ggattggacg 900
tctaatgtgg aatacggaag aagagcccgt tgatgagact atgagatcaa gagcatttca 960
agaaagtatt cgattgatgt tccgcacgga gaacagcgaa tataataata tacaagcgat 1020
aggcagtggc agaacgcaga ggcagaagtt ttatgcacct ctaataatcg cagcgaccga 1080
tagttttagg tggcgcatat ggtggaacaa cccatacccg tgtctacgtg gttgcctgat 1140
agcatgtgag atggagctag gtgacgttta caaaagtcta aaatcgatct ataagtggac 1200
cttacgctca agctattctt cccagcgtga aatagataga caactcaacg tatttccgta 1260
tcagaagatc aatttattcg atttttccgg aacaccaggc actgaaataa tacattggag 1320
aattgttcac atacctgtgc cggatgagat cgattatgaa catggatatt tatgcccaga 1380
aagtggagat tatgacattg tcatggccgt tgatgatgat ttatacagcg cttttaagca 1440
tagagtaatt gatcgggggt gggagcatca ggccatgaag ttagatgaat tggtaccaca 1500
ggagaataat atatttaaaa tggaattcga aaaagatgcg taccttgaca atcgtaacca 1560
cttggttttg cctccgtatt ataacaaagt agtatattgt cctttgtttc acgccacagc 1620
aaagattaca ccgacagaaa tttctcatcg ccaaagcgac gacccatggt gcgaacgaac 1680
gttgtatggg ttcaggggcg atcatgtcat aacacattat actggactga atcacattgt 1740
gaaccgacgg aagcctctca tgggtcagac gttatcggtg gcccaaagta tgggagacta 1800
tactaaatat atattaggcg acgatgggtc agctaacagt tgcacaggga ccgcgtattc 1860
gtcgcgatta ccaacaatgt tgtttcagga tatctttgac gcgattattc gacatcttcc 1920
aaaagttata ttgaaggacc aagctgatta tttctccgag tcgaggaaac gtgttagtga 1980
tatcattgat tgggatactt ttatcgtgta tagtatctac gcttgctttg aaaggaagaa 2040
tcaagttcta agggaagagg atgcgacgca gattgtccga ggaataagca cggcgggaaa 2100
agagaaaaga cttaagttga ttgaaagaag ttttcccatt tactatcgag aactgatgaa 2160
gatcaagaat gcgaagacgt ggaaggacgc ttacgccttt aatttcttac cattactatt 2220
gtgtgctggc acgaatgtgc aatacgtaca cagacagtgg tcctatccgg tattggtttc 2280
gatggagaag ggaccgcgta ttattcctgc gatggtggga agggatttgg cagatgggaa 2340
tctaggcgat tggcacgcat acattaagta ctatatggga cagcatatgg aagaccaaga 2400
ggtggaggag gacagcttgg agattctaaa gatttcgctt gaacattata tgtctcacag 2460
ttatatctct aacgttaagg atgttgcggt caggatgacg aaggagattg ttttagattt 2520
atacttcgcg tctgggtgca atggagtaag tgaaatgatg accttcctat taccaattat 2580
acatccgcgg aaaggtttgg tgatgttttg tatagcggat gaccaagtgc aaccgcaggt 2640
tcaatgtgag acggcgcttc gtagatttcc ttatacgcgc gaatatatac atgacgtcat 2700
tgttatccag gttgggaggc cgggtgagct taagcaagtt tggaagcgcg gtagcactaa 2760
agtgaaagta tgtcgacgaa attttttgag gtatgatcac aaagtcattc tgtctaggtt 2820
atgtgggtta gtttatggta actatgagtt gatgaccaag cttacaaata tatgagctag 2880
tgacatagct cacccgcaac taaacttac 2909
<210> 110
<211> 1639
<212> DNA
<213> 人工序列
<220>
<223> BTV15 AJ586716的VP5 DNA
<400> 110
gttaaaaagt ccttggtatc ctgcatcatg ggaaaaatca ttaagtccct cggaaagttc 60
ggaaagaaga tcagtagtgc attgggttcc agtacagcaa agaaaattta ttcaacgatt 120
gggaaagcag ctgaaaggtt cgtagaaagt gatctcggca gcgcaactat agatggcatt 180
atacagggca gtgtgcattc agcgttgacg ggcgagtcgt atggggagag cgtaaaacag 240
gcggtaattc taaatgtagt gggtggaggc gattcaatag ctgacccctt aagtccgggt 300
gagaacgaca tggtgcatcg tctgaagagg ttggaggatg agcagaaggg agaaattata 360
cgtacgaagc acaatgcgca ggtaatcgct aaattcgggc gcgatcttga agatgtctac 420
aaatttgcgt cacgagaaca taaagtcgga gaggaggagg aggaccagat ccagatgctg 480
gaaaaagcgt tgcgagcgta cgggaacgta gtgaaggtcg aaggagaaag cgttcagaaa 540
ttagcgaagg ccttacgcgt ggatgaggag gaacgcaccg atgaagagac acgtatgatt 600
ggagagtacc gtgataaaat tgatgcgcta tcaaaagcca tagagataga acgtgaaggt 660
ttacaggatg aagcaataca ggagattgcg gggatgagcg cggaagtact tgaagcggcg 720
gcagaggaag ttccgatttt tggcgctggg gtggcgacaa gtatcgcaac agcgcgagcg 780
atagaaggtg cttataaatt gaaagcggta ataaccgcct tgaccgggat tgatctaagt 840
catatgacaa ctccaaagat ccaacctaaa acgttagaag ccatattgga tgcgccgggg 900
gggaatgtaa cggacttgat gctgacgcgg gggttggact caaaaattga caagatcaac 960
gagaatctgg ctgaagtgga acatatgcag acgtcaattt taccacgcat caaaaaagcg 1020
attgaagagg atagagatga aattacgaat tggtcaccga agcggataca tccgaaaagc 1080
gtccaaaggt ttcgcgtgcc tcgaatgcaa acgccatcga ttcatattta tgcggctcca 1140
tgggattcgg acagcgtatt catctttcac gtaatctcgc cacatcattt gaacgaatca 1200
ttcttcttgg gctttgattt agaaatcgaa tatgtgcatt acgaagatct ggcgcaacac 1260
tggcattcgt tgggagctgc acaggaagtt acgggcagga ctttccgcga ggcgtatcga 1320
gagttcttta acctggcgtc gagatcaact atggcgagtg acattcacaa gaaacgttta 1380
caacgctccc gcatgtccca tcctatttat ctaggtgttc ataattatga gctttcatac 1440
atcgcgatta aaagtaatgc gatgcaattg gtgacggatg aagaccttca aaaacatgtc 1500
ctcagagggc cgctacactt ccagcgacgt gtgattatgg cagctttaaa atatggtgta 1560
aaggtgatgt cgagggcgga tgatatcgcc atgatgctca gagacgcgtg aaggcgggga 1620
gacaacgact tacacttac 1639
<210> 111
<211> 2935
<212> DNA
<213> 人工序列
<220>
<223> BTV16 AJ585137的VP2 DNA
<400> 111
gttaaaaacg ttagcctaga gatggaggag ctagttatac cagtcataac tcgtcaattt 60
gataaaaaac tggttgggcg ttacgattac gtcattgaac tgacggaacc agaggacggc 120
gagtggagtg gccacgatgt cacagagatt ccaaatagac gcatgtttga cattaagcag 180
caaaatatca gagaggcaat agaatacaaa cctgtagata acgatgggga agttttgccg 240
cgcatcttag atatgtctat tgcgtgttac gacatgaaaa agagcatgat gaagaaggat 300
ggagtcgatt ttgtatccaa cacgagatgg ttagaatgga tgatagggga ttcgatggac 360
gtgcaacctc tgagagttca actgaaagaa gatcacagta caatacaata cgggatgttc 420
tcaaacacgt tacacatcga ttcgaggaaa gcggatacca cgtcatatca tacgattgct 480
gtagaatcaa aaggggagcg tgggtgttgt cacgttcaca cggcaatttg gaatcacatg 540
gtacgtaacc acttgtttaa cgccgttcaa gaggcgtgct acgtgtttaa accgacgtat 600
gatttaatag tgattggtga aaagcaaaat cgtgaggatg aatttaggat tggcgaacat 660
aatttttaca ccataacacg gaatcaccac atgcgtttag gcgataacgc gtataatcaa 720
ttcatgaagg gtttggttca gttgcgcgtg gcgggagtga caccgaacgc gatacgagag 780
gaaatggccg ctttagacgc gataagagat acttggatag gagggaactt cgagcgaaca 840
cacatcaagt cccttgaaat atgtaagtta ttatctagca ttggaagaaa gatggttaac 900
atggaggaag aaccaaagga tgaaagagac ttatcagtta agtttcaatt taaactcgac 960
gacaaatttt caacaaccga tccagaaaga aacgttattt ttacacacaa aacacaccgt 1020
acgaatcaag atcgttttta tgtgtcgcta atgattgcgg cgtcggacac aaataatggc 1080
agagtatggt ggtcaaaccc ttatccatgt ttacgtggcg cgttaatcgc gtctgaatgc 1140
aagctcggtg acgtgtacca taaattacgg gcgtggtatg agtggagcgt aagacctgaa 1200
tataaaccgc gagatttgga gcgagaacaa gaaaaataca tcgtcgggcg cgttaacctc 1260
tttgacttag aaggagagcc tgcaacgaag gtatttcact gggagtacga attgattaat 1320
aaagtgtatc agataacaaa tcatacggga aatcattgtg atttgtatcc tgatgatgta 1380
gaaatcacgg ctaaatttga tgaggagaaa tacggagaga tgattcaaat gataatcaac 1440
gaaggatgga aacacggtga ctttaagatg tttaagattc tgaaggagga gggtaacccc 1500
ttactatatg atctagagaa ggacattagg ttagatagta gatcacaagt tatatttcca 1560
ccatatttta acaaatggac gcacgctcca atgtttaatg caaaagtgaa accatgcgaa 1620
gtcgagttag cgcaacgaaa gaatgaagac ccttacgtaa agcgaacggt taagcctata 1680
cgtgcggatt gtatcgatct gttgaggtat cacatgtcgc attacatgga tgtgagagtg 1740
tcaatgaagg ggcttagttt agcagttaaa cagacgccat ccagtattca tcaagaatgg 1800
gcgaaggatc cattatatcc aggttttctg cagaggagag atgaaaattt agatcataag 1860
tcggtatgtc cgatcgtcac aaactatttc tggttagaga ggttctatac tttagtttta 1920
actataatgg aaaagcatta ttgggattta gacgatagtg atgttacgta tgagttcccc 1980
acattggatg attcagctta caagattgag ggaacgttgt atgatgtctc gcaggtggtg 2040
gttcatatga ttgatagatt ttttgagaag aggcgtttct tacgctcagt cgatgagtgc 2100
aggtggatat tgcatttaat aaggtccacc cgaagtcggg acaggctgag cgtttttaaa 2160
cgctttttcc caacctttgg tgagggactg tgtgtgaaca actttcgtaa agtgaaagat 2220
attatgctat taaatttcct gccttttttc tttttaatcg gcgacaatat ggcgtatgaa 2280
catagacagt gggcggtgcc tttgctcttc tacgcagata aaataagggt catacccgct 2340
gaggtgggcg cttattataa tcgtttcgga ttggtgagca tcttagagct gctaacattc 2400
tttccctcgt atgaaatgcg tgataataag gttgaggagg atgtgcacgc gtgtgcgagc 2460
gcgatcctgg atttctatct aacgacaact atttcaaatg gaggtataca ggctagcata 2520
gtttcaacta aggcgttact ttatgagacg tatctatcct cggtgtgtgg cggtttttct 2580
gaggcgattc tgtggtacct cccgattacc catccagtta agtgtttagt tgcgcttgag 2640
gtgtcggact cgctagtaga ctcaaatgta cgtatcgaca aaatcaaacg aagatttccc 2700
ttatcccata gacatttacg aggcattgtc cagatctctg tacggccaga tcgcaccttc 2760
ggcgtgacga cgtgtggtat tgtaaagcat aaaatatgta aaaagactct gctgaagcag 2820
cggtgtgatg taattttaat ccagatcccg ggttacgttt tcggtaatga tgaattactc 2880
acgaagcttc taaatattta atcccgtgac agggattggg cacgtttaca cttac 2935
<210> 112
<211> 1637
<212> DNA
<213> 人工序列
<220>
<223> BTV16 AJ586719的VP5 DNA
<400> 112
gttaaaaaga tccctacgat tgcggaagat gggtaaaatc atcaaatcac taagccgttt 60
cggcaagaag gttgggaacg cgttaacgtc taatacagcg aagaaaatct atagcaccat 120
agggaaggcg gcggaaaggt tcgcggagag tgagattggg tccgcggcaa ttgatggact 180
agttcaaggt agcgttcact caatcatgac cggggagtca tatggcgaat cggtgaaaca 240
agctgtgctg ctgaacgttc tgggggcggg ggacgagatt cctgatccgt taagcccagg 300
ggaacgcggc atccagatga agatcaagga gattgaagaa gagcagagaa acgaattggt 360
aaggctgaag catgggaagg agattacaaa gaaatttggt gaagaactag aggagatata 420
tcaatttatg aatggtgagg tgaaggatga ggaagagcag gaagagcaat ataaagtgct 480
atgtaaggcg gttaactcgt acgagaaatt acttatagct gaaaatgaca agatgcacat 540
attagctcgt gcattacaga gggaggcagc tgaaaggact gaagctgaat caacaatggt 600
taaagagtac aggcagaaaa ttgatgcact aaaggccgcc atcgaaatcg aacgggatgg 660
gatgcaggag gaagcgattc aagaaattgc tggaatgaca gcggatgtgt tggaagcggc 720
ttcagaggaa gtacctttag ttggttcggg gatggcgacc gcgattgcaa caggaagagc 780
gatcgagggc gcgtataaac taaaaaaggt gataaacgca ttaagtggaa ttgatttatc 840
tcatttgcgt acaccgaaga tagaacctac gatggttgcg actacgttgg aacatagatt 900
tgaagatata ccagacaaaa aacttgctat gagcattcta gccaaaaata acgcgataac 960
ggcaaacacg agagagatcc agcatattaa agaggagatt ttaccaaagt tcaagaaggt 1020
aatggatgaa gaaaaagagc ttgaaggaat tgatgataag aagatccacc caagagtaat 1080
gatgaggttc aaagtgccac ggtcgcaaca gccgcaaata cacatatata gtgcaccatg 1140
ggactcagat gatgttttct tctttcattg tatttcacat ttccatgcca atgaatcttt 1200
cttcttaggt tttgatttag gaatcgatgt agttcatttt gaggatttgg cggcccattg 1260
gcacgcttta ggcgcggcgc aggaagctaa aggacgtacc ttgaacgaag catatcgcga 1320
attcttaaat ttagcaatcg gtagtaccta tacttctcca atgcacgccc gacgtatgat 1380
tcgatcgaaa acggtacatc caatctattt aggatcgatg cattatgata taacgtacga 1440
tactttgaag tcgaatgcgc agaggattgt atatgatgat gagttgcaaa tgcacatatt 1500
aaggggtccg ttgcatttcc aacggcgcgc aatactgggc gcgttaaagt ttggagttaa 1560
ggttcttggt gataaaattg atgtcccgct cttcctaaaa aacgcgtgac tgcggtaatc 1620
tcgggactta cacttac 1637
<210> 113
<211> 2923
<212> DNA
<213> 人工序列
<220>
<223> BTV17 AJ585138的VP2 DNA
<400> 113
gttaaaagag tgatccacca tggaggagtt cgtcattcca gtctattcag aagatgagat 60
tccatatgcg ttactaagca gataccctct ggcgatacag acaaatgtta aaatagagga 120
tgttgaagga aaacataatg ttgtaaagat tcctgaatcc gatatgatag atataccaaa 180
attaacgatt gtagaggcga tgaattataa accagcgaga aacgatggga tcgttgtacc 240
tagattactt gatataacat tacgcgctta tgatgataga aaatcgacga aaagcgctcg 300
agggatagag ttcatgacga acgctagatg gatgaaatgg gccatagatg atagaatgga 360
tatccaacct cttaaagtga ctttagatca ttattgttcc gtcaaccatc aactctttaa 420
ctgcgttgtt aaagcgaatg ctgccaacgc tgatacgatc tattatgatt atttcccgct 480
tgaggactat aaaaagagat gtaaccatac aaatcttgat ttattgagaa gtttaacgaa 540
tatggagttg ttccacgcgt tgcaaggtgc tgcgtacagt atcaaatcaa gctacgaatt 600
agtggcatac tccgaaagag gaagcctgga ggagacttat gtggtaggac agccaaaatg 660
gatacacttg actagaggaa cgcgaatagg caatagtgga ttatcctatg aacggttcat 720
ttcgagcatg gtccaggtaa gcgtaaatgg aaagattcca gacgagatag cgaacgagat 780
cgcgcaactg aatagaataa gggcagagtg gataacagcc acatacgata gaggcaggat 840
tagagcgcta gagctatgca atatcctttc cacgattggg cgtaagatgc taaatacgca 900
tgaggagccg aaagatgaaa tggacctatc gacgagattt cagtttaaac ttgatgagaa 960
atttaacaga gcggattcag aacatgttaa tatttttggt gtaagaggtc cagcaacaga 1020
tgaaggaaga ttctacgctc tgatcgcaat cgcagcgaca gatacacaaa agggcagagt 1080
gtggagaacg aatccgtatc catgcttgcg gggtgcttta gttgcagctg agtgtgaatt 1140
aggtgatgtt tacagtacac ttcgacgtgt gtatagatgg agtctaaggc cagaatatgg 1200
acagcacgag cgacagttag agaacaataa atacgtcttt aatcgtataa atttatttga 1260
ttcaaactta gcagttggtg accagattat tcattggcgt tatgaggtta aagcatcggc 1320
ggagacgacc tatgacagcg gatatatgtg tcggcatgaa gctgaggagg atgaactatt 1380
atgtaaaatt aatgaggaca aatataaaga aatgctggac agaatgattc agggtgggtg 1440
ggatcaggaa agatttaaac ttcataacat actgacggac ccaaacttat tgacgattga 1500
ttttgaaaaa gacgcgtatc tgaattcacg gtccgagtta gttttgccgg attatttcga 1560
caaatggatc agttcaccaa tgtttaacgc gcgcttaaga attactaaag gggagatcgg 1620
aacatcaaaa aaggatgacc catggaacaa ccgcgcggta cgtggataca ttaagtcccc 1680
tgcggagtcg ttagattttg ttctcgggcc ctactacgat ctgcggcttc tattttttgg 1740
cgagacgttg agcttaaaac aggaacagtc cgcggttttt caatatttga gtcagctcga 1800
tgattttccc gcgcttacgc agctaacagg agatgccgta tgcccacatt caggcggagc 1860
gttatatacg tttaggaaag tcgcgctatt tttgatcggg aattatgaaa agttaagtcc 1920
ggatctacat gaaggtatgg aacaccagag atatgtgcat ccgtcgactg gcgggacgta 1980
tcagaaacgc gtgctagaga tgaaggatcc ctgccagctt acgtgttttg tgattgatta 2040
catctttgaa aaacgtgaac agctacgtga taccaaagag gcgaggtaca ttgtgtatct 2100
aattcaaagt cttactggga cacaacggct gagtgtttta aggtcgacgt ttccgaattt 2160
cttccaacga ttgttgatgc taaaagagat caaatttgtg cgcgatctga atgtgattaa 2220
cttcctccct ctgatgttcc ttgttcatga taacatctcg tattggcata gacagtggtc 2280
gattccaatg gtactgtttg acgatacgat caagttaata cctgtagagg ttggtgcata 2340
tgcgaataga tttgggttca aaagttttat gaactttaca cggtttcacc ctggtgagtt 2400
gaagaaaaaa cagattgccg aagatataca taaagagttt ggtgtggtcg ctttcgaata 2460
ttacaccaat acaaaaattt cccaggggaa tgtgcataca ccagtaatga ctacgaaaat 2520
ggatgtattg agagtacatt tatcttcttt atgtgcaggt ctggcggact cagtcgtata 2580
tacattacca gttgcgcatc ctaaaaaatg catcgttcta ataattgtgg gagacgacaa 2640
attggaaccg catacgcgtt cagaacagat agttagtcgg tataattact cacgtaagca 2700
catttgtgga attgtatccg tcactatcgg gcagaacagt cagttgagag ttcatacatc 2760
tggaattgta aaacaccgtg tatgcgacaa gttcattcta aaacacaagt gcaaggtgat 2820
attagtgagg atgccggggt acgttttcgg aaatgatgaa ttaatgacga aactattgaa 2880
tgtctaggtc ctatgacacg gaccgatggc ctcttacact tac 2923
<210> 114
<211> 1638
<212> DNA
<213> 人工序列
<220>
<223> BTV17 AJ586720的VP5 DNA
<400> 114
gttaaaaagt gttctcctac tcgcagaaga tggggaagat aattaaatcg ctaagtagat 60
ttgggaagaa ggttggaaat gcattgacgt caaacacagc gaagaagatt tattcaacca 120
ttgggaaagc agcggaacga tttgctgaaa gcgaaatcgg tgcggcaaca atagacggtt 180
tggtgcaggg cagtgtccat tccataatta caggtgaatc gtatggagag tcagttaaac 240
aagcagttct tctcaacgtg ttaggtacag gtgaagagtt accagaccct ctgagccccg 300
gcgaacgtgg tatccaaacg aaaataaagg aattagaaga tgagcagcga aatgaacttg 360
ttcgattgaa gtataacaaa gagataacaa aggagtttgg gaaggagtta gaggaagtct 420
acgacttcat gaatggcgag gcgaaggagg aggaagtggt tcaggaacaa tactcaatgt 480
tatgcaaagc agtggattct tacgagaaaa tattaaaggc agaggactcg aaaatggcaa 540
tgttggcacg cgcactgcag cgggaggctt cagagagaag tcaggacgag atcaaaatgg 600
taaaggagta cagacagaag attgatgcgc ttaaaaatgc gatcgagatt gaacgagacg 660
gaatgcagga ggaggcgatc caggagattg ctggaatgac cgctgacgtc ttagaagcgg 720
cttcggagga ggtgccctta atcggcgcag gtatggccac tgctgtagca accggcaggg 780
caatagaagg cgcatataaa ttgaagaaag ttataaatgc gttaagtgga atcgatttgt 840
cgcatatgag gagtccaaag attgaaccaa ctattatcgc tacaacattg gagcaccgat 900
ttaaagagat accagatgag cagctagcag taagtgtgtt gaataagaag acagccgtaa 960
ccgataactg caatgaaatc gcgcatatta aacaagaaat attaccaaag tttaagcaga 1020
ttatggatga ggagaaggag attgaaggaa tagaggacaa agtgattcat ccgcgggtga 1080
tgatgaggtt caagattcct agaacgcagc agccgcaaat ccacatttat gcggctccgt 1140
gggattctga tgacgtgttt ttctttcatt gcgtttcaca ccatcatcgg aatgaatcct 1200
tcttcttggg atttgattta ggaatcgatg tcgttcactt tgaagactta accagccatt 1260
ggcacgcatt agggttagcg caagaggcga gcgggcgtac gttaacggag gcgtatcgtg 1320
aatttctcaa tctatcaatt tcaagcacgt atagtagtgc gatacatgcg agacgcatga 1380
tcaggtcacg agcagtacat ccgatctttt taggatcaat gcactacgat attacatatg 1440
aagctttaaa gaataatgcg cagagaatag tctatgatga ggaactgcaa atgcatattc 1500
taaggggacc tttgcacttt caacgccgag ccattctggg agcgctgaaa tttggagtta 1560
aaatattagg cgataaaatt gatgttcccc tcttcttacg aaatgcatga acgcagcgag 1620
ggagatgctt acacttac 1638
<210> 115
<211> 2927
<212> DNA
<213> 人工序列
<220>
<223> BTV18 AJ585139的VP2 DNA
<400> 115
gttaaaatag cgtcgtgatg gatgagttgg caattccaat ctacacagat acgtttccgg 60
cgggtctatt agatggatat gattatatca tagatgtgag tactcgtgta gatgagccta 120
aggggcgtca cgatgtaact gagattccta aaagatcgat gtttgatatt aaggatgaac 180
ctatacgtga agctatcata tacaaaccag tcagtaacga tggatgcgtc ttgccgagag 240
tgcttgatat cactttaaga gcatttgatg ataggaagag ggttattacg aatgaaggcc 300
acagcgaatt tcacacgaga tcaaattggt tgcagtggat gatagatgat gctatggatt 360
atcaaccact taaggtcgac gtagagcatg taccatcacg gataagccac gccctatcca 420
atagcacggt aaggttacac gcaaagagag cggatactgc gtcttatcat atagagccag 480
ttgaagtgga ggcgtatggg tgcaatcacg tgtggaccga aagaatccat cacttagtaa 540
atattgaaat gttccacagt tcgcaggagg ccgcttatat attgaagcct acatatcaaa 600
taattgcaca cgccgaacgc gcggcggtgg gagatgcgtt taatggtagg acgctcgagt 660
tgaatcggaa tcatcgcata cagttggggg atcaaggata tgtgaaactc aagaaaggat 720
gggtccagac tagaatagag gggaagaaac ccgcagctat tgagagagaa atagcatctc 780
tgaatgaaat tagagcgcag tggatagcta gaggccatga ccgacgagaa atcaaggcat 840
tagacttgtg caggattttg tccgcaattg gaagaaagat gtataatata gaggaagaac 900
caaaaaatga agcagacatg tcggtaaagt ttcagatgga gttggacgaa atttttaaac 960
ctggtaatag cgaacacgac aacatcatgg gtggtggcac gcacaggaag gacgaagata 1020
ggttttatgt gttaattgtt atcgctgcgt cggatacaaa tcagggacga gtttggtgga 1080
gtaatccata tccgtgctta cgtggagcgc tcattgctgc agaggtgcaa ttaggtgatg 1140
tttacagtac attaaggagt tggtatcagt ggagtatcag acccgcgtat gttccatacg 1200
atagaagcag agaaaatgat aagtatatct atacccgaac taatttgttc gattccgctt 1260
tgggacctgg tgataaaata atacattggg aatatcagct tcttaatgaa acgcgagagg 1320
tcagcatgag tagcggtaac gaatgcgatt tgttcccaga agatgaggaa ttcaccacga 1380
agttcaatga agagaggtat acagagatga aaggtcagat catccaaagt gggtggaacc 1440
atcgagattt taaaatgcat aaaatacttg aggatggtgc aaacgtgtta acaatcgatt 1500
ttgaaaaaga tgctcgtgta gggactgggt ctgtgttgac attaccagat tattatgata 1560
agtggatcgt ggcacccatg tttaatgcga agctgcggat cacagaaacc gttatcgcga 1620
atgctcattc ggatgatcca gcgattgagc gcagcgtgaa gccatttgat catgacccat 1680
caacgttaca aagattttgt ttggctagat attacgatat aagacctgga ttaagagggc 1740
gagcgctgct caaacagcag gatgggtcaa ctataacaaa gaaggtttcg gcactggatg 1800
attatcagga tgttgtttcc cgacgcgtga actatgttca gcgtgatgtg aggtgcccta 1860
cggagacagc acagtatgtt ttagagaaaa catcgctata tctattagca ttgtttgata 1920
aacatgtgat gccgtccgcg gagtcagaag ctaagtttgt gcacccttca atcgatgtta 1980
agcacgatat cgagacgtgg cgcatcgctg atatttcgca attgattgtt ttgattttcg 2040
attatctctt tgagagtcgc aaggtggtga gggatgctgt ggaggcgaga tggattcttt 2100
ataaaataag atcatcaacg ggacgcgcga ggatggaggt gatcgaaacg acattcccac 2160
gatttggact aactgtaaag aatatcacaa aggcgaaagt gaatcaagat attgcgtgtt 2220
taaatttctt tcctcttttc ttcatcgtcg gtgacaatat ggcgtatgct caccgccagt 2280
ggagcatacc tatattactg tacgctcatg acattcgcat aatacctctc gaggttgggg 2340
catatagtaa tcgctttgga ctcacgtctt atttggaata tatgattttc ttcccatcct 2400
acgcaacgcg gatggctatg atcgatgagg ctaataaaga gtgtgcggct gcgttgctcg 2460
actattatct gagtacggat gtgcatcgtg gaggagtcat gacaagtgtt atcacgacga 2520
aacgtctact gtatgagacg tacctatcct cgttgtgcgg agggtactct gacggtttgt 2580
tatggtatat tccgataaca catccgctga aatgtttggt ggcgtttgaa gtcgctgatg 2640
acgttgttcc agctactgtt aggaaggaaa ggattctaat gcgatttccg ttaagtgcta 2700
ggcatctaag ggggatagcg ttgataacga ttgatcggga tcgcaaggta catgtacaga 2760
cggagggtat agtatcgcat agactttgta aaaagaattt acttaaatac gtatgtgatg 2820
tcattctttt caagttttcc ggttatgtat ttgggaacga tgagatgctg acgaagttgc 2880
tcaacgttta gctctcgtga ctgggagcgc acgcgctatc gacttac 2927
<210> 116
<211> 1637
<212> DNA
<213> 人工序列
<220>
<223> BTV18 AJ586721的VP5 DNA
<400> 116
gttaaaaaag cgatcgcttt cgcgaagatg gggaaaatca taaagtccct aagccgattc 60
ggaaagaaag ttgggaacgc cttaacatca aacacagcaa aaaagattta tagcacaatt 120
gggaaagcgg ctgaacgatt tgcagagagc gaaattggct cagcggctat tgatgggtta 180
gttcagggga gcgtacattc gttgatgacg ggagagtctt acggcgagtc ggtaaaacag 240
gctgtgctat taaatgtaat gggaagtggt gaagagcttc cagatccact aagcccgggt 300
gaacgtggaa tgcagacgaa gatccgtgag ttggaggatg aacagcgcaa tgagttgatt 360
cggttgaagt ataatgataa gataaagcaa aaatttggaa aagaattaga agaggtatat 420
gagtttatga atggggtcgc gaagcaggag gaagatgaag agaaacatta tgatgttctc 480
aaaaaagcgg taaactcgta cgataaaatc ttaaccgagg aggaaaaaca aatgaggatt 540
ttagcaacag cgttacagaa ggaggtgaag gagcggacgg ggacggaggc cgtcatggta 600
aaagagtatc gaaataagat cgacgcatta aaagaggcta tagaggtaga gcgtgatgga 660
atgcaggaag aggcgattca ggagatagct ggaatgacgg cggatgtgtt ggaggcagct 720
tcggaggagg tgccactaat aggtgcagga atggcgacag ctgtagccac gggtagggct 780
atcgagggag cttacaaatt gaagaaggtc ataaacgctc tcagcggaat cgatttgacc 840
cacttaagaa caccgaagat tgagcctacg atagtatcga ctgtgctgga tcataagttt 900
aaggatattc ccgatgaaat gctcgctgta agtgttttat caaagaatag agccatcgaa 960
gagaaccata aagagattat acatcttaag aacgagatat tgcctagatt taagaaggca 1020
atggacgagg agaaggagat atgtggaatt gaagacaaaa agatacatcc gaaagtcatg 1080
atgaaattca aaattccgcg aactcaacag ccccaaattc atatatatag tgcaccgtgg 1140
gattctgacg acgttttctt cttccattgt atatcgcatc accacgcgaa tgaatcattt 1200
tttattggat tcgatttagg tatcgatctg gttcactacg aagatttgac tgctcattgg 1260
cacgccttag gtgcggcgca ggcggcggtt gggaggagtc tgaacgaagt gtacaaagaa 1320
tttttgaact tagcgattaa taacacgtat agctcgcaga tgcacgctcg gaggatgata 1380
cggtcgaaga ctgtacaccc catatatttg ggctcactcc attatgatat ttcgttttcg 1440
acattacgga gcaatgcgca gagaattgtg tatgatgagg aattacagat gcacatatta 1500
agaggaccac tacacttcca aaggcgggcg atactaggcg cgataaaaca tggagttaaa 1560
atcttaggga cggaggttga tatcccactc ttcttaagaa atgcctgagc gcagcggagc 1620
caccgctttc cacttac 1637
<210> 117
<211> 2947
<212> DNA
<213> 人工序列
<220>
<223> BTV19 AJ585140的VP2 DNA
<400> 117
gttaaaaagg atttcgccac aatggatgag ttcgcagtac ctatagttcg tgggcgcacg 60
ataccgtatg acttgattag agcatatgag actattattg atctagatgt taaagtggag 120
gagaatgaag gtaaacatga tatcacgaaa attccagaat cgtacatgtg tgatgtccct 180
gatataccaa tacaggatgc tacaacagct actctgagta ggaacgatgg ctatgcgttg 240
ccgcgcattc tggatgtgtc tatcgctgct tacgatacgc ggaaaggtta tcaaaaatat 300
aatggtatag aaaggatgac aaatcaggtg tggttaaagt gggctataaa ggattttatg 360
gatgtgcagc cgctgaaggt tagtttaaca gacgctgaag gaaaggttag atatgattta 420
tttaactgct cagtgcggat ggatgcgaaa aaagctgaag cagtatacta tgggtgggcc 480
gcgcaggagg ttatcgataa gggatgtaac catgcaagaa gtgaattact tcagcatgtc 540
atcaggtgtg aactgtcaaa ctgtgctcaa gaaagcgcgt atatgctcaa gcctacgcag 600
aagttggagc ctgttgcaga gaaaacatcg cttagtgaga ggctgctgcc gggatcgagt 660
catctaacta gtatgcggcg tggtgaggtg ttcaggatgg gtacagagca gtataatagg 720
atggtgcagg gtatggtatc gagtcaaggg gtaaaaggcc acacacctgg taaaacaaga 780
attcaagatg aaatgaatga gttaatgaaa ataagagatc gttggaaggt tgcgagagac 840
cggagagaga tcaaatcact tgagttatgc aaactattat cgtcagtcgg gaggaagatg 900
tacgacaagt acgaggagcc gaaggatgaa gaagctatgt cgttgaggtt tcagtggaat 960
ctcgatgaaa ttttcaagac cgataatcgg gagcacataa atattgcggt tgaaaagagc 1020
gggaggacta atgatgaaag attctttgca ttgatcatga ttgcagcgac agatacgaat 1080
cgagggagga tctggtggtc gaacatgttt ccgtgtttgc gaggcgctct gatagtagga 1140
gagacgcagt ctggcgacgt gtataaaatg ttacgtgttc gattcgactg gagtgttcgc 1200
aatgagtaca gtacgcaggc tgatgtcgca cggcagaatg acaactatac gtttacgcgc 1260
gttaatctat ttgatttgat ttctgaacca gcaacccctg tgatacattg ggagtatgac 1320
ctggatgcac cacgaaaaac aaattattta gatggacatg catgcgatga gttcccggat 1380
gattatgatt tagtatgctc gtttgatgaa actaagtata gcgaaatgat tcagagaatc 1440
ctagacggtg gatgggatca taaaaacttt aagtattata agattctgaa agatgcgggg 1500
aatgtactca cgattgattt tgagaaagat gcgaggctta atgaacattc ggaaatggta 1560
atgccatcat acttcaataa gagaattgcc gcgcctatgt tccgatgtat cctgagaata 1620
tgtgaaacaa cgatttccgt gagacaaaac gcggacccag ctgtgtctag gaccatcagg 1680
ccgcttaaag cgaaggctgc tgatctacta gattttaccc taggctccta ctatgacaca 1740
cgttttggcg taatgggaag gacgttagtg atgtcacaaa aacagagcga aatgtataaa 1800
atgattcaag aagcggagga ttacagcgaa ctgatgaagt cgcggggtgt catcgaaaag 1860
ttcaaaaacc catgcccaat tacgacagcc tgtcaatata ccttgcagaa gacctcggtg 1920
ttgatccttt cccgagctca gcgttatttc agagaatttc acggtgggga ccaagaagaa 1980
tacatgagtc caacattgaa ttttgagtgg tccgatatga catcaaatat ctatgatgtt 2040
gcgcaaatgg ttgtattagt tttcgacgta ctattcgaga gaagacgcgt tgtacgtggc 2100
gtagatgagt cgaggttcat tttacatcag ttaagaatta gaaagggatt aaatcgctta 2160
gattatgtga aaatccactt tccacacttg tatcaatggt ggaaggcctc agaatctacg 2220
tatggagagg cgcagaaatt aaacctgctg cctttgctct ttctcgtagg ggataacttc 2280
atctatgaac atcgccaatg ggcgtaccct gcagtgttga tatcgcataa attacggttt 2340
attccggttg agctcggtgc gtactataat aggtttggat tgacttcttt gatttcgcat 2400
ctgactttcc acgtggggaa agcgaagtat gatcaaaatc tcgatcaagc ggagcagaag 2460
gttttcagcg cgcttaagga ttattatgcg gaatcaacta tcacagatgg gggagttaac 2520
acgcactcga cgatgacaaa agatccgata taccaggttt acttatcgac tatctgcggt 2580
ggattgcttg attcacttgt ctatctaatg cctatcacac accctatcaa aagtatcgtt 2640
gctatagtta taaatgatgg aaaaggctcg gcgtcagatt gcgttcgacg tgtgacgcgg 2700
aaactcaggt atattcaaca tcatatacgc ggatccgttg tgatctcgat tcttagtgat 2760
ggctcaatca acacgtattc agaggggatt gcgaaagcga cactgtgcga taagagcgtt 2820
ctaaagtata gttataaggt ggtactgatt aaggttaaag gtcacgtatt tggtcatgat 2880
gagatgctca caaaactgct aaacgtgtga ggcttccgtg actgcaagca ggcgacattc 2940
aacttac 2947
<210> 118
<211> 1637
<212> DNA
<213> 人工序列
<220>
<223> BTV19 AJ586722的VP5 DNA
<400> 118
gttaaaaagt gtcctcatca tcgcagagat gggcaagatc attaaatcat taagtagatt 60
cggaaagaaa gtggggaacg ttctgacgtc aaatacggca aagaagattt acagtacgat 120
tgggagggcg gcagagcgtt tcgctgagag tgaggtgggt gcagccacga tagatggatt 180
cgttcaggga agcgttcact cgatcatgac gggcgagtct tatggtgaat cggtaaaaca 240
agcagtactg cttaatgtgc taggcagtgg tgaagagtta cctgatcccc taagtccagg 300
agaacgcggg atacagacca aactaaaaga gttggaggat gagcaacgca atgagctggt 360
ccgtttgaaa ctgaataaga aaatagttga caaattcgga actgaattgg aggaggttta 420
tgattttatg aacggggcgg cgaaagatga tgagaatgaa attaaacaat acgatatttt 480
gtgtaaagct gtagactcat acggaaagat cttgcgagag gaagacaaga aaatgttgga 540
attagcgaga gcgttgcagc gtgagtcgga gtcgagaagt gacgaggaga ccaagatggt 600
gcgggaatat cgttcgaaaa tcgatgcgtt acgcgatgcg gttgacgttg aacgtgaaag 660
catgaccgaa gaagcgatac aagaaatagc aggaatgaca tcagatgtcc tagaggcggc 720
ggcggaggaa gttcccttgg ttgggtcggg aatggcgacg gcaatagcga cgggacgcgc 780
gattgaaggc gcgtataaat tgaagaaggt aataaacgca ctaagcggta ttgacttgac 840
tcatttacgt acgccaaaga tagaacccag cttaatcgct acaacattag agtatagaga 900
taggccaatt ccggatgact cactagcagt cagcgtactc gagaagagga gggcgataga 960
agaaaataaa aatgagatcg aacatattaa aaatgagatt ttaccccggt ttaaaaaagc 1020
gatggaggaa gagaaggaga tttctggaat cgaggagtct gtgatacatc ctaaagtgat 1080
gatgagattc aagatcccca gatctcagca gcctcagatt cacatttatt gttcaccgtg 1140
ggattcagac actgttttct ttttccattg tgtatcacat catcatatga acgagtcctt 1200
ttttatcggt ttcgacttag agctagattt agtgcatttt gaagatttga ctgcgcattg 1260
gcacgcttta ggcgccgcgc aaacggttgt tggacgcacg ttccaagagg cgtaccgaga 1320
gtttttaggt cgtgcgatga tggatccttc aagcggagcg atgcataaga gaagaatgtc 1380
acgatcccgt agtgtacatc ccatttatct tgggtcgatg cattatgata tatcctacgc 1440
taccttgaag agtaacgcac agcggatagt ttatgatgat gagttgcaga tgcacgtgct 1500
gcgtgggccg atacactttc agcgcagggc aatcttaggt gcgcttaaac atggcattaa 1560
aatacttaat gataaaattg atctcacatc tcttcttaga aatgcgtgaa gcagcgacga 1620
caggcgctta cacttac 1637
<210> 119
<211> 2925
<212> DNA
<213> 人工序列
<220>
<223> BTV20 AJ585141的VP2 DNA
<400> 119
gttaaaagag cttcccacaa tggaggaatt cgtaatccca gtattctcgg agcaggacat 60
gccttatgct ttgattaatc aatatcccct tgcgatacga actgatgtta aaataccaga 120
cgttgagggc atccatgatt tagctaaaat acctgagtcc gatatgatag atgtcccgaa 180
attggatata gtaagcgctt taaactataa acctgtgagg aatgatggca ttgtggtacc 240
acggctattg gatatcactc tgaaagctta cgacgatagg aagtcaacga aggacgcgca 300
aggcattaaa tttatgacga atgccggttg gatgaaatgg gcgattgacg ataggatgga 360
tatacagcct ctgaaagtaa aattagacca acattactcg atagattacc aactcttcaa 420
ttgtgttgtg aaggctaaaa cggcgaatgc ggatacgatg tattacgact acttccctat 480
tgagaataaa acgaaaaaat gtaatcacac gaatttggat ttgttgagga gtttgacagc 540
tgttgagatg tttcacgtac tacagggggc tgcctacaat ttgaaagcca attatgaatt 600
aatcgcaaac tcagaaagag aaagtctcga ggagagctat ccaatcgggt cccagaaatg 660
ggtgcatttg actagaggaa ctaaaattgg aaatagcggg gtaccgtatg aaaggttcat 720
ctcaagcatg gttcaggttg tggtaaaggg taaggttcca gttgaaatcg tgagcgaagt 780
ggtgcggttg aatgagatta gaactgagtg gataaacgca gggttcgacc ggagccgaat 840
tagagcatta gagttatgca acattttatc cacgattgga cgcaaaatga tggacacaca 900
agaggaacca aaagatgaaa tggatctgtc aacgagattt cagttcaaac tagatgataa 960
gttcaataca gcggattctg aatacatagt tatatcaaga gtaggagggg cggcgacaaa 1020
cgaaggaaga ttttacgccc tcattgccat tgctgccaca gatactcaga gggggagggt 1080
ctggaggacg aatccgtatc cgtgtctacg cggtgcattg atagcggcgg agtgtgaatt 1140
aggtgatgtc tactactctt tacgtcacac ttatcaatgg agtctgcgcc cggaatatga 1200
tcagcgagag agatcgctag aaaacaacaa gtacgtgttt agtcgtataa accttttcga 1260
ttctaaccta acggtgggag acaaagtcat acactggcga tatgatctga ctaagccaat 1320
ggatacgact tttgatgacg gctatatgtg tgtgtcgcat gaaatagatg gtgagttact 1380
ctgtagaatt gacgacgaca gatacaagga aatgctggaa agaatgatac agggcggttg 1440
ggaccaggag cggttcaagc tccatagcat attgaccgaa cctaacctat tgactattga 1500
ttttgaaaaa gatgcctacc ttaattcgcg gtccgagttg gtgatgccgg attacttcga 1560
taaatggata aattcaccca tgtttaatgc gcgattgcgg ctaacccatg gagagattgc 1620
gtcgcgtaag actgacgacc cgtggaacaa ccgagtcgtg tttggataca tcaaaactac 1680
gactgagtcg ttggactaca tgctagggag atactatgat gtgcggctgc aattttatgg 1740
tgatacattg agtcaggggc aacgtcaatc ttctgtcttt gcatacatta gtcaacgaga 1800
cgatttccag acgcttactc actacgtaaa ggatgatgca atatgtccgc atgctggagg 1860
gtcgatttat actttccgta aaaccgcttt gatgctgatg gctaattacg aaaaattaag 1920
cccggagctt catgaaggat tagaacatca aacttatacg catccttctg acggaatgga 1980
ttatagaaag tatgttgtag atatgaaaga tctctcgcag ttaatatgtt tcgtgtttga 2040
ttatattttt gagaaacgcg cacagttacg cgatcctaag gaggcgagac gaatcttgta 2100
tttggtccag aataatgtag ggtctcagcg gttgggagta ttgagtagtg cattcccaaa 2160
tctcgctaat cggttgttaa agctacggga gatacatttc atttacgata tgaatgttat 2220
caatttctta cccctgttgt ttcttgtcca agataatatt tcatattggc atagacagtg 2280
ggctatacct atggtactct ttgacgaaac catcaaacta attcctgttg aagttggcgc 2340
ttatgcgaat cggtttggat tcaagagctt ctttaacttc acgcggttcc acccaggcga 2400
ttccaggaag aagcagattg ctgacgacgt acataaggag tttggtacaa tatgctacga 2460
atattacatg aatactagga tgtcacaagg tggagtgaat atatcagtgg tcactactaa 2520
gttggatgtg ctcaagatac acctcgcttc actatgcgcg ggattagctg attcgattgt 2580
ctacactttg ccggtcgcac atccgaggaa atgtattgtt ctgatagtgg ttggtgatga 2640
caaacttgaa ccacagatcc gcgcggaaca ggttctcagc aaatattatt attcgcgcaa 2700
gcacgtgttg ggcgttatct caatcagcgt tagccaagac ggtaagttaa aagtttactc 2760
gtcgggcatc gttagacatc gcctctgtga gaaatctata ttaaaatata aatgcaaaac 2820
tatcttaatt agaatgcctg gacacgtttt tgggaatgat gagcttatga cgaaattact 2880
caatatttag gtccttgaca cagacctgtg gcctcttaca cttac 2925
<210> 120
<211> 1638
<212> DNA
<213> 人工序列
<220>
<223> BTV20 AJ586723的VP5 DNA
<400> 120
gttaaaaagt gctgtcctac tcgcagaaga tggggaagat tataaaatcg ctaagtaggt 60
ttggtaagaa ggtaggtaac gcgctgacat caaataccgc gaagaagatt tactcaacga 120
tcggaaaggc ggcggaacgt ttcgcggaaa gtgagatagg tgcggcggct attgatggac 180
tagtacaggg aagtgttcat tcaattatca caggagaatc atatggtgaa tccgtaaagc 240
aggcggtact attgaatgta ttgagcgcag gtgaagaact accagacccc ttgagtccgg 300
gtgaaagggg catgcaagcg aaaattaagg agctagagga tgagcagcga aatgaattaa 360
ttcgattaaa atataataag gagataacgg atgcgttcgg aaaggagttg aatgaagtct 420
atgaatttat gaatggcgaa acaaaagagg aagaagtcgt acaagagcag tactcgatgc 480
tatgtaaggc ggttgattca tatgaaaaaa tactaaaagc tgaggactca aaaatggctg 540
tcttagcgcg cgctctacag cgagaggctt cggagagaag cgaggatgaa atcaaaatgg 600
tgaaagagta tagacataaa atagatgcgc tgaagaatgc gattgaggtc gaacgtgacg 660
gaatgcaaga ggaggcgatc caagaaatag cgggaatgac tgccgacgtt ttagaggcgg 720
cgtcagaaga ggtaccattg gttggcgcgg gcatggcgac cgccgttgct acgggaagag 780
ctatcgaggg cgcgtataaa ttaaagaaag ttataaatgc gttaagtggg attgatttat 840
cacacatgag gaccccaaaa atcgagccta ctatcatcgc tacaacactt gagcatcgat 900
tcaaagacat acctgatgat cgattagcta taagtgtgct gaacaagaaa gctgcagttg 960
ccgataactg taatgagata tctcatataa aacaggagat tctaccaaaa tttagaaaaa 1020
ttatggatga agagaaagag atcgaaggga ttgaagacaa agttattcac ccacgggtaa 1080
tgatgaggtt taagatcccg agaacgcaac aaccacaaat ccacatctat gctgctccct 1140
gggattccga cgaggttttc ttcttccact gcgtgtcaca tcaccaccgt aatgagtcct 1200
tttttctggg ttttgattta ggtatagacg tagtacattt tgaggatctt accagtcatt 1260
ggcatgcatt aggaaatgcg caagaggcaa caggacgtac gcttacggaa gcatataggg 1320
aattcttaaa tttgtctgcg acgagtatgt tcagcagcgt aatacatacg agacgtatga 1380
taagatcgag agcagttcac cctatatttc ttggctcgat gcactatgat gttacgtacg 1440
aggttcttaa gaacaacgct cagcgtatcg tttatgatga tgagctacag atgcatattc 1500
tgagaggacc attgcatttt caacgtcgag ctatattggg ggcgttgaag tttggtgtga 1560
agattcttgg cgataaattg gatgttcccc tcttcttacg aaatgcatga acgcagcggg 1620
ggaagcgctt ccacttac 1638
<210> 121
<211> 2922
<212> DNA
<213> 人工序列
<220>
<223> BTV21 AJ585142的VP2 DNA
<400> 121
gttaaattag tctcgtgatg gatgagttag cgatacctat cattgatggt cgattcccga 60
aggaggcatt attggaatat tgttgtatag ttgagctaaa tccagcgagt gaggaagatg 120
atgtggatgt gacaacgata ccgcagaagg atatggtgga tataccggac gcgccaatac 180
gcgaagctct gaactactta cccaagagga acaatggcca cgtattaaca cgcgttgtgg 240
atatatcatt gagcgggtac gatagacgaa aataccatcg gactagcaca ggtggagatt 300
tcttgtctcc cgaggaatgg ctgaactgga tgatccgaga ttcggtggat gtgcagcctc 360
taaaagcgag cttagaattt ccgtcaaaga ttaaacatag tttatttagt tccaacgtgt 420
atgtgtcagt taagaaagcg gacaccatat cgtatcacat agaagcgaag gaagttgaaa 480
cgagggcgtg tgaccacgcg cgtgtagcaa tgtggaacaa tcttgtgaga catgagatga 540
tgcatagtgc gcaggagcca gcatacattc tgaaacccac gctcgacata atcgtgcatt 600
cagagagggc atcgacggat gttccattcg aactagtcgg tcaacgctat atctcgattg 660
ggaaaaacca tcgaatagtt ctaggagatg atgcctatcg aaagacatta gacggactta 720
caaggttgcg tgtacaaggg cgaattcctg agttaatacg aaatgagata caacaacttc 780
accagataag ggatgcgtgg ataaccgatt cgtacaaccc acgacacata cgatcgttag 840
aactgtgtag aatcttatcg catataggaa ggaaaatggt gcatatagag gaggaaccta 900
aagatgaaag tgatttatcg gttaaattcc agtttaagtt agatgaaaaa tttagaatta 960
atgatgatga gcgtaacgtg atattcacat caaagggtca aaggaatgat cagcagcggt 1020
tcttcgtatt aatcatgatt gcggcatcag atacctacaa tagccgtatc tggtggacaa 1080
acccataccc atgcctacgt ggcgccttga tcgcctcaga aacctcactt ggggatgtgt 1140
attggacgtt gagacatttc tatgattgga gcgtcagacc ggtatattca ccgagacaaa 1200
aggagcgaga agacgataaa tacttatatg gtagagtcaa tctatttgat cttgatgccg 1260
atccaggtac acagattata cattgggaat ataagcctat tacaagtacg gtgaaaacta 1320
cgtatgagag aggcaatatg tgtgatttat ttcctgatgt agacggcatt acgactaaat 1380
tcaataaaac ggcttacatt gatatgatta gcgaattgtt aaatggtggc tggaatacac 1440
gcgaatttaa aatgtataaa atacttgaaa gcgtagggaa cgttttgaca atcgattttg 1500
agaaagatac gaagcttaac tcaagatctg aactttcatt accgtactac tatgataaat 1560
ggatatacgc tccaatgttt aatgccagat taaagataac gcaaacagag atcgctcaac 1620
gtaaagcaga tgacccgagt atatatcgta ccttagcgcc aattagttcg gaccctatcg 1680
atttacagcg catgtgttta gctaattact atgacgtaag accggccatg catggacgtg 1740
cgctttctaa aagacaagaa caatccgctt atcacaaaaa tttagcgaac ctagccgatt 1800
atcaggatat tttaaaacgt agaggatacc gtaaagttga gaacccatgt ccgctgatca 1860
cgtgctcgta tattttggaa aaatctgcat tactttggat tgatattatg gagaaacacg 1920
taggctacgc gccggagagc gagatagagt ttgattttcc gaaagtgaac acgtctactc 1980
gaatcggtac tgagagaatc gttgatatat cacagctagt cgtacttatg attgattggc 2040
tttacgagaa acgccaaact acgcgaagtg tggatgaagc gcgatggacc gtacaagtaa 2100
ttcgacgcgt tactgggaag aagaggctgg aggcgatcag tcgcgagttt cccaattttg 2160
gagaagcggt ctcgcgcctt gcatgtccca aaactgttag agatgtaatg gtaatcaatt 2220
ttttgccttt cctcttctta ttaggtgata atatggcgta cgaacatagg cagtggtcgg 2280
ttccgattat attatatgct gataccttat ggttccttcc tgtggaggta ggagcgtatt 2340
acaatcgttt tggaatcgta tcgtttttag aatatttaac cttcttcccg tctaatcaca 2400
tgagaacagt agcgttggat gaagcggaat ctaaggtggg gaagttgata ttagaatttt 2460
acttgaatac cactatttat cttgggaaca ttcagcaaag tgtaatctcg acgaaacaaa 2520
ttttatatga aacttatctg tcttcgctgt gtggaggctt tactgaggga ttagtttggt 2580
acctcccaat aacgcatccg caaaaatgtg tagttgccat tgaaatttgc gatgaccgag 2640
tcagcgcggg ttacaggtgt gataagctac gatcgaggtt tccgctcagt ctggatcact 2700
tacgaggtat cgtgatactg tccgtttcca agagtagggc gattagcgcg tactcagagg 2760
ggatcgtttc gcaccgattg tgtaagaaaa acgttttggg tttcacgtgt agagttttgc 2820
tactgaaatt ctctggtcac gtatttggga acgatgagat gctaactaaa ttgctaaacg 2880
tttagcccta gtgacatagg gtgcacggac taatcaactt ac 2922
<210> 122
<211> 1637
<212> DNA
<213> 人工序列
<220>
<223> BTV21 AJ586724的VP5 DNA
<400> 122
gttaaaaaga tccctatgat tgcggaagat gggtaaaatt atcaaatcac taagccgttt 60
cggcaagaag gttgggaacg cgttaacgtc taacacagcg aagaaaatct atagtaccat 120
agggaaggcg gcggaaaggt tcgcggagag tgaaattgga tctgcggcga ttgacggaat 180
agttcaaggt agcgttcact caatcatgac tggggagtca tacggcgaat cagtgaaaca 240
agccgtgcta ctgaacgttc tgggggcagg ggatgagatt ccagatccgt taagcccagg 300
ggaacgtggc atccagatga agatcaagga gattgaagaa gagcagagaa atgaattggt 360
aagactaaaa tatgggaaag agattacaaa gaaatttggt gaagaattag aggagatata 420
tcaatttatg aatggcgaag tgaaggacga ggaggagcag gaggagcagt ataaagtact 480
atgtaaggcg gtgaactcat atgagaaatt acttatagct gagaatgata aaatgcgcat 540
actggcgcgt gcattacaga gggaggcggc agaaagaact gaagctgagt caacaatggt 600
taaggaatac agacagaaaa ttgatgcact aaaagctgcc attgagatcg aacgggatgg 660
gatgcaggaa gaagcgattc aagagattgc tgggatgacg gcagatgtgt tagaggcggc 720
ttcggaagag gtacctttag tcggctcggg gatggcaact gcaattgcaa caggaagagc 780
aattgagggc gcatataaat tgaaaaaggt gataaatgcg ttaagtggaa ttgatttatc 840
tcatctacgt acaccaaaga tagaacctac gatggttgcg actacactgg aacatagatt 900
tgaagacata ccagataaaa aactcgctat gagcattcta gccaaaaata acgcgataac 960
ggctaacacg agagagatcc agcatatcaa agaggagatt ttaccaaagt tcaagaaagt 1020
aatggatgaa gaaaaagagc ttgaaggtat tgacgataag aagatccacc cgagagtgat 1080
gatgaggttc aaagtgccac ggtcgcaaca accacaaata cacatatata gtgcgccatg 1140
ggattcagat gatgtcttct ttttccactg tgtctcacac ttccacgcca atgaatcttt 1200
cttcttaggt tttgatttag ggatcgatgt agttcatttt gaggatctgg cggcccattg 1260
gcacgcttta ggtgcggcgc aggaagctaa agggcgtacc ttgaacgaag catatcgcga 1320
attcttaaat ttagcaatcg gtagtaccta tacctctcca atgcacgccc gacgcatgat 1380
ccgatcgaaa acggtatatc caatctattt aggatcaatg cattatgata taacatacga 1440
tactttgaag tcgaatgcgc agaggattgt gtatgatgac gagttgcaaa tgcatatatt 1500
aaggggtccg ctgcatttcc aaagacgcgc aatactaggc gcgttaaagt ttggggtaaa 1560
ggttcttggt gataagattg atgtcccgct cttcctaaaa aacgcgtgac tgcggtaatc 1620
tcgggacttt cacttac 1637
<210> 123
<211> 2907
<212> DNA
<213> 人工序列
<220>
<223> BTV22 AJ585143的VP2 DNA
<400> 123
gttaaaagtt gccaggatgg aagatttccc gatttgtgca atagagcgcg aatccgtata 60
tgatgaggat tttataatga gataccctgt cattgtggac ctaaaggagt gtgtggcgga 120
tagaagaaac aaaaccgatg tgtcaaagtt agaagaaatt aaaggcgtat gtgtgcggtc 180
ggaaggcgta cgcgtagcgt tgacatcaag accagaccct ttatcacatg ttatcacgcc 240
atccgcactt aacgtgctaa tccaagcgta tgatcgtaag cataaagtat cgctaaggaa 300
gcgcttcgaa gatggtgagg gggtgaccga gtatgatttg gacacgtggg tcaagaaacg 360
attggaggag caagcggact tacaagcggt ataccacacc atagatgcaa accgagggaa 420
attgaaattt aaaacattga tgggaacgat cagattggat tccaactatg ctgaaactgt 480
agggtatgac tacgcgccag tttcgaaggc tggttgtaac cacgggaatg aggatccgtt 540
gtatgatttc attctgtcag gagcgttcca ggtatcgaag acagcaggat acacgttaaa 600
acaaacatat aggctgactg tgcgtacaga ggttcgtgaa aataacaaaa atgagttggt 660
cgtcaacgaa atttatactc ccggtatcgt acgcggcgaa cagataagag atatgacgag 720
tgttgcatat atgaggttcc gcgatggatg gatacgagcg atcattcagc cgcagatagt 780
agaagagctg agagcgagca aggacgcgtt ggataggata tcaaatgaat ggtatagcag 840
tcgaaatacg ccagatgttg ttgaaatatg tcgaattgtt tcatcgatag gtcgccggat 900
gtggaacagt gaagagacgc ccgttgacga cgctatgagg tcgagggttt ttcaagagaa 960
gttgcgacta ttattccgag tcggaaatag tgaatatgat acgattcaat cgatctcaag 1020
cggccaaaca gatctaaaga agttctatgc gctattagcg atagcggcga ccgacagtta 1080
tcggtggcgt atctggtgga gtaatcctta tccatgcttg cggggaacta taatagcatg 1140
tgaaatggaa ttgggagatg tttacaagac tttgagatct acgttccgat ggagcttgcg 1200
accagagtac gcgaatagat ccgagattga tcgagagaag aaggcgtacc catatcagag 1260
gataaattta tttgaatcaa aattgacccc agggacgcgc attattaatt ggctggtcac 1320
tcgagtgcca cttgaaaaac cggacattag tacgggacgc gtttgtatga cgtcaactga 1380
atccaattat attttgaaaa tcgatgatgc gaggtataag cagatgataa gtcagatcat 1440
tgaaagaggt tgggaaaaag aacagttgaa atttgaaaag atagttcaag atgatggaaa 1500
cgtattcaaa atggaatttg aaaaagacgc ttgcatggat gaaaagagtc accttgttat 1560
gccatattat tatgataagg aaattcattg cccgatatat catcaaaaaa ttaagatcac 1620
agaggcgcag atggcaaatg agtggagtga cgacccatgg gtgaataggg tcgtaagcgg 1680
atatttgggc gaccatgttg aaacatatgg tttagggtac aaccatattt ttgatacacg 1740
cgtaccattg aagggtaact tattggaaca agagcaagag atttccagct atcatgagca 1800
tctgcgctca gatgaatcaa aggaggatga cactcattgc gcgctgcgaa tcttttcaga 1860
taaagctatc cgtatgttgt ttatagctat atataagaaa ttgattcatc acatccctaa 1920
ggaagaatat gctacacgcg aggaagagct gattcagtac atttctatgg ttgagagggt 1980
tcgatcatgg gatcaattca ttgtattcat ctttgatttc ttctttgaaa acagaaaagg 2040
gatcaggaca atggaggagg caacacagtt ggcaaaatct atacgtggta gcgtaggcgc 2100
gatgcgatgg acgatatttt cgagttcatt tccaactagt tacagactaa tgaaacaaat 2160
tttggtggcg aagaggtggg gtgactgcta cattatgaat tttttaccgc tgttgatatg 2220
cggtggtact tccattagct acttacaccg tgaatggagt tatcccatga tagttacgtt 2280
tgaagacggg atcaagattg tgccgacaat ggttggacga tcactaccgc agggatcttt 2340
gggagagtgg gtatctttta taagcttttt tatggggact gtgcacgaga aaatcaaagt 2400
tgaagaagat gagctagaag tcctgcggcg gtgcgtggaa ttttacagag atgtgaagat 2460
ggtcgatgta attaaggaca ctccagtgcg aatgacgaag tacataacat gtgatctcgc 2520
gtttggtagc ggttgcggcg gtataagtga atctattagt tttatattac cggtcacgca 2580
tccgaatcgg agtttagttg tctttgtgat ctcggatgat aagttatctc catcccaaca 2640
tgcggcgagg gctaaacgta ggtttaaagg tacatgccag tatatagatg atgttattgt 2700
tcttcaaatg aagcggcccg gattggttgg gagtgtttgg agcgagaagc acagcaaaat 2760
caaagtctgc aggcgtaatt ttttaaatta tgatcataag gttatattaa cgaaattcgc 2820
aggtttagtt tacgggaact acgagctgct cactaaactg gctaatctgt gagcctgtga 2880
cacggctccc tagcaacttc aacttac 2907
<210> 124
<211> 1645
<212> DNA
<213> 人工序列
<220>
<223> BTV22 AJ586725的VP5 DNA
<400> 124
gttaaaaagc agccaccaaa cattgcatga tggggaagat catcaagtcg ttgggcaaat 60
ttggcaaaaa agtcgggaac gcgttgacat cgaagacagc gcaaaaaatt tataaaacta 120
tcggaaatgc agctgaaaga ttcgccgaaa gtgagattgg aagcgcggcg atcgacggat 180
taatacaggg aagtgtacat tcaatcatga caggagagtc gtatggcgag agtgtcaagc 240
aggcggtgat attgaatgtt ttaggaggag gcgacacgat agctgacccg ctgagcccag 300
gtgagaatga aatttataga aagttgagac agctcgagga ggaacagaaa ggtgaagtag 360
tgagaacaaa acataacacg gagatattgg ataagttcgg aagggatctc gatgaggttt 420
ataagtttgc gacccaggag cataaggttg gcgaagatga ggaggatcag attaaaatgc 480
tcgagaaggc gttgaagtct tatgtgggcg tctttaaggc ggaaaatgag agcattaata 540
aactcaaaca ggctttgtac ctcgaatcaa cagaacgtac ggatgaggag acgaaaatga 600
ttagcgaata tcgcgacaaa attgatgccc tacggggggc gattgaggtc gagaaagagg 660
ggttacagga ggaagcaatc caagaaattg ctggtatgag cgcggatgtt ttagaagcag 720
cggcagaaga agtaccgtta tttggagcgg gtgttgctac gagcattgcg actgctcgcg 780
caattgaagg cgcgtacaaa attaaggctg tgataagcgc tctgtctgga attgacttaa 840
cccatctgag tgtgcctaag gtccagccca aaacgttgga agcgatccta gatactccaa 900
aggggaaaga catatctgat ttacgattga ccgaaggctt gatagcgaaa atggataaga 960
tatctgaaaa tatctcagag gttgatcata tacagaacgt gattgttccg aaacttaaaa 1020
aagcagcaga ggaggataaa gaaaacataa caaattggtc tgaaaaacgc attcatccca 1080
aaacggtgca acgttttaag ataccacagg cccaaaggcc ttcaatgcat atatatgcgg 1140
cgccctggga ttcagatgaa gtgtttatct ttcacataat ttcacctcat cataagaatg 1200
agtctttctt tttagggttc gatttggaaa ttgaatacgt tttttatgag gatctgactc 1260
agcactggca cgcgttgggg gcggcgcaat cggcgtatgg acgtactttc cgcgaggcgt 1320
atagggaatt tttcatgctt gcatcgaatg tggctggaca aggggcgatt catcaaaaaa 1380
ggttattgag gtcgaggagt agtcatccta tctatttggg gatgcatgat tacgaagttt 1440
catacgagca gatcaaaatg aacgcgatgg cattggttag caacgaagat ttacagatgc 1500
atgtactgcg cggccctgtg cattttcaga ggaggacgat tgtggcggca ctgaagtatg 1560
ggattaaaat acttacccaa ccggatgacg tttccgtcat gcttagaaac gcgtgaaggc 1620
gggtgtaggg gctacttcaa cttac 1645
<210> 125
<211> 2927
<212> DNA
<213> 人工序列
<220>
<223> BTV23 AJ585144的VP2 DNA
<400> 125
gttaaaatag cgttgcgatg gatgagttag caattccaat atatactaat gtatttcccg 60
cggagctgtt agatggctat gattacatta tcgacgtaag cacacgtatc gatgaacctg 120
cgggacgtca tgacgttaca caaattccaa attcagcgat gtttgacgta caaaatgagc 180
acatccgaga cgccatcata tctaaacctg ttagcaatga cggatatgtg ttgccacgaa 240
tactagacat ttcgttgaga gcgttcgacg atcggaaaag aataataacg agtggaggtc 300
agagtgaata tcatacgaaa gcggattggt tacagtggat gatcgacgac gcgatggatg 360
tgcagccgct caaggtcgat actgcgcaca cgagaacgcg agtgagccat gcgttattta 420
actgtacggt taggctgcat gcgaaaaaag cggatactgc ttcgtatcat gttgagtcca 480
ttgaaatcga gccgaatggt tgcaatcatg cgtggattca aagagtgcac catttggtca 540
acatcgagat gcttcactgt tctcaagagg ttgcctatat gctgaagccc acatatgaaa 600
cgattgtcca cgccgagcgc gcatccacta gcgatacttt taacggaagg acgatagagc 660
tgagccgtgg tcatcgcgta caattgggag acgctgcgta cgagaaactg aagaacgggt 720
tggttcaaac acggatcgaa gggaaaaaac cacaattggt cgaaggcgag ataaattccc 780
tgaatgatat aagacagaga tggattaaca gcggccacga tcgaagagag ataaaagctc 840
tggaactttg taagatcctg tccactatcg gacgaaagat gctcaataca gaagaggagc 900
caaagaacga agcggattta tcagttaaat ttcagatgga gttggatgag atcttccgtc 960
ctggaaataa cgaacgaacg aacatatttg gagggggtaa tcatagaaag gatgaggata 1020
gattttacgt gttaatcatg atagcggctt ccgatactta taagggtcgt atatggtgga 1080
gtaaccctta cccatgttta cgcggatcat tgatagccgc tgaagctcag ctcggagatg 1140
tttataatac aatgagaaat tggtatcagt ggagtgttag gccaacttat accccgtacg 1200
ataggagcag agagagcgac aaatacgtat atagccgagt gaatctgttt gattcaacgc 1260
ttagacctgg cgataagatt atacattggg agtataaatt gctcaacgaa gtacgagagg 1320
tgtgcataaa ggatggtaat gaatgtgatc tattccctga agatgaagag atgaccacca 1380
aatttaatga ggaaaaatat acagaaatga agggccagat cattcaggat ggatggaacc 1440
atcgagattt taaaatgcat aaaatactgc aagatggtgc taacgttttg acaattgatt 1500
ttgaaaaaga cgcacgtata ggaaatggat cagttttgac gttacccgaa tattataaca 1560
aatggatagt tgccccaatg ttcaatgcga aattacgcat ctctgagact gtaatcgcga 1620
aagcacactc agatgaccct gccgtagcta ggacagctaa atcgtttgat catgacccct 1680
tcaacttaca acggtactgt ttagcgagat attatgatgt acgaccagga ttgatgggac 1740
gagcattatc caaagagcag gaagggtcag ccatagcgaa caaagtggct gcgcttgagg 1800
attatcggga cgttatatca aggcgtttag ggtatgttga acgtgaaaaa cgatgtttaa 1860
ctgaaacggc gcagtatata ttggagaaaa catcattgta tctgttaaac gtactgagta 1920
tgcacgttaa accgactatt gatatggata ttaaatttaa gcacccatcg attgatacac 1980
accttgatat tgatacctgg aagatcgcgg atatttctca acttatagta ttactatttg 2040
attatctgtt tgaaagtcgt aaggagatta gaagcgtgac tgaagcgagg tggattttgt 2100
ttaaggtcag atccgtttcg ggacaagaaa gactgaatat gattcaatct ttctttccac 2160
ggtttggacg agtcgttaga gacatcgcta aagcgaaaat cactcaggat gttgcgcatc 2220
ttaacttctt tcccttattt tttatcatcg gcgacaacat ggcttacgct catagacagt 2280
ggagcatacc tgtcctatta tacgctcatg acataagaat aataccatta gagattggtg 2340
catataacaa ccgatttggc tttacatcct acttggagta tatgacgttc tttccgtcac 2400
acgcgacacg agaggcggta cttgatgaat caattaaaga gtgttcgctc gcattaacta 2460
atttttattt gaataccgat atccatagtg gagagatcat gcgcaacgtg gtgacaacga 2520
agcgcttgct atatgaaaca cacttagcgt ctttatgtgg tgggtattcg gatgggttgt 2580
tatggtatgt tcctataacg cacccgtcaa aatgtttagt tgcctttgaa gtggcggatg 2640
acgttgtgcc atactcagtc cgaagagaga gaatcatttc aaggttccct ctcagctcta 2700
aacatttaag gggtatcgca ttaatcgcgg tcaatcggaa tcagaaagtg tcggtgcaaa 2760
cagagggtat agttacgcat cgactttgta aaaagaattt attaaaatat gtatgcgatg 2820
tggtcttgtt caaattctct ggttacgtat ttgggaacga tgaaatgtta acaaagttgc 2880
taaatgtata actctcgtga cagagagttc gcacgttata cacttac 2927
<210> 126
<211> 1635
<212> DNA
<213> 人工序列
<220>
<223> BTV23 AJ586727的VP5 DNA
<400> 126
gttaaaaagt gtaccctgag cgaagatggg taaggtcata cgatccctaa gccgatttgg 60
caaaaaagta ggtaacgcgt taacttcaaa cactgcaaag aaaatctaca gtacgattgg 120
gaaggcagct gaaagattcg ctgaaagtga aattggatca gcagctatag atggattagt 180
gcagggtagt gtacactcga taataacggg tgaatcatat ggtgaatcgg ttaaacaggc 240
tgtgctatta aacgtgctag gtagcggtga agagattcca gatcctctaa gcccagggga 300
gcggggtatc caggcaaaac tcaaagagtt ggaggatgag cagcgaaacg aacttgttcg 360
tttaaagtac aatgacgaaa ttaagaaaaa atttggagaa gagttggaac aagtttacga 420
tttcatgagt ggggctgcaa aagaggaagc tggagatgaa aaacaatttg atattctgaa 480
taaagcggtt aattcatata ataagatatt agctgaagaa gacttgcaga tgcgacgact 540
tgcaacggct ttacagaagg aagtaggcga gagaacacgt gtagaaacta ctatggtgaa 600
ggagtatagg gataagatcg atgctctacg aagcgcaatt gaggttgaga gggatggaat 660
gcaggaggaa gcaatacaag agatagctgg gatgaccgcc gatgtgttgg aggcggcgtc 720
ggaagaagtt cctctaatag gagcggggat ggcgacagcg gttgcgactg ggagagctat 780
cgagggagca tacaagctca aaaaagtgat aaatgctcta agtgggattg atttgacaca 840
tctacggacc ccgaagatag aaccaactgt tgtatccact atattagaac atcgtacaag 900
ggatatcccg gataatgcgt tggcggtaag cgttctatcg aagaaccgtg tcatacaaga 960
aaatcataag gaactcatgc atattaagga agagatattg ccacgtttta aaaaggcgat 1020
ggacgaagag aaggaaatat gtggaataga ggataaggtt atacacccga aagttatgat 1080
gaaatttaag attccgagag cacagcaacc acatattcat atttatagcg caccatggga 1140
ctcagatgac gtgttcttct ttcattgcgt ttcacaccac catatgaatg aatcattttt 1200
cttaggattt gatctgagca tcgatttggt tcactatgaa gatttgacag ctcattggca 1260
cgcattgggc gcagcgcaga tggcggtagg acgcacgcta actgaagcgt ataaggagtt 1320
tcttaacctg gcgatctcta acgcctacgg cacgcaaatg catacgagac gtttgatcag 1380
atcgaagacc gtacacccaa tctacctggg atcgcttcac tatgatgttt cgttcgcgga 1440
tttgcgaggg aatgctcaac ggattgtgta tgatgacgaa ctacagatgc atatactacg 1500
tgggccgctc cattttcaga gacgagcaat attgggtgcg ttgaaattcg gatgcaaaat 1560
acttggggat cggttggacg taccactctt cttacgaaat gcttgaacgc agcgatgggg 1620
agcacttaca cttac 1635
<210> 127
<211> 2924
<212> DNA
<213> 人工序列
<220>
<223> BTV24 AJ585145的VP2 DNA
<400> 127
gttaaaagag tgacccacga atggaggagt ttgtcattcc agtttattcg gagaacgaaa 60
ttccatatac gctactgaat agatacttgc ttgcgatacg aactgatgtc aagattaatg 120
atgtggagaa aaagcacaat gtgatcaaaa tacctgattc tgacatgata gatataccaa 180
aaatcagcat agtagacgca ctcagttaca gacctacaag gaacgatggt attgttgtgc 240
caagattact tgatataaca ctacgtgcgt atgatgatcg gaaggcaacg aagaatgcga 300
aaggggtaga gtttatgact aatgccaagt ggatgaagtg ggcgattgat gacagaatgg 360
atattcaacc tttgaaagtc acgttggatg accactattc tgtgaatcac cagctattca 420
attgtgtagt aaaagcaaag ccagctaacg ccgatacggt ttactatgat tattatccac 480
tagaaagtaa gatgaagaaa tgcaaccaca cgaatttaga catgttacgt agtttaacga 540
ctatggagat gtatcatata ttgcaaggag cggcttatgg tttgaaatcg acttatgaat 600
tgaaagctca cgcggagcgg gagaatacgg gtgagagtta tccgataggg gaaaggttgt 660
tacaaatacc cagaggaacg aagataggaa gtagaggaga accttataat aaattcgtcg 720
ctggattggt aaaagtgact ataaaaggta aagttcctgc tgaaatcggg gaagagatga 780
atcaattaaa tcgaatcaaa gaggagtgga agagcgcatc gcacgacaga agcaggatac 840
gtgctttaga actatgtaaa attctatcaa cgattggccg aaaaatgctg gatgcgaagg 900
aagagccgaa ggacgagatg gatttatcta cacgatttca attcaaacta gatgagaaat 960
ttaccaaaac tgactcagaa catataaaca tattcagagc aggtggaccc gctacagatg 1020
acggtagatt ctacgcttta atcgcaatcg cggcaactga tacgcaaaaa ggtagggtgt 1080
ggaggatgaa tccgtatccg tgtttacgcg gcgcaataat agcggccgaa tgcgaactag 1140
gtgatgtgta tttcacgctg cgtcaggtgt acagttggag tttgcggcca gagtatggac 1200
agaaggaaaa accattggaa gtgaacaagt atgtatttaa tcggattaat ctgtttgatt 1260
cggacctaac agtaggagat cagatcgtac attggcgcta tgagttatat cccccggttg 1320
agacgactta tgatgatggg tatctatgtg gatcagataa agaggacgat gagttattat 1380
gtgaagttga cgaggagaaa tataagaaga tgtttgaaaa tatgatcgag ggtggatggg 1440
accaagagag atttaagctt catagcattt taactgaccc gaatctatta acgatagatt 1500
ttgaaaagga tgcgtatctc aattcaaggt cggagctggt atttcctgaa tatttcgata 1560
agtggatcaa ttctcctatg ttcaacgcga agttacgact agcccatgga gaaatcgcaa 1620
cgcgcagagc aaacgaccca tggaacaatc gggcggtgca tggatacata agaacatcca 1680
ctgaatccct tgggtatgct ttgggtagct attatgatct aaggctacag ttattcggtg 1740
acgcgttaag tttggaacaa cgacagtcgg cggtctttga acacttgaat aaacaggacg 1800
atttcttcac gctaaccggc tacgcaaaag atcaagttgt atgccctcat tccggcggtg 1860
cgttctatac gttccgcagg gttgcgctgg agatcttatc gaactatgag aagcttacgc 1920
cggacttaca tgaagggctg gaacatcaga cttacacgca cccgaagata aacgaatatt 1980
ttaatagatt tgttctagat atgaaagact tttcgcaatt gatatgtttt gtattcgatc 2040
attttttcga aagacacgag cagttacgag atgtggcgga agcgagaagg ttggtctatt 2100
tgatccaaaa atctgacggg cctgagcggt tggacgtcct ccagaggaac tttcctaact 2160
tttttcgacg tttcatgaat ttgaaggacg tcaaatatat atacgattta aatgtggtca 2220
actttttacc gcttcttttc cttatccaag ataacatatc atattggcat agacagtggt 2280
ctgtcccagt gatactttat ggcgatatta ttaggttaat cccggttgaa gttggagctt 2340
atgcgaacag atttgggatg aagagcttct ttaattttac tcgatttcac cctggagatt 2400
caaagaagaa gcagaacgcg gatgatacac ataaggagtt tgggctgata tgctttgagt 2460
attacacaaa aactaagatt tcacaaggtg gggttgatat atccgtggtc acaacgaaga 2520
tggacacgtt aaaggtacac ttagcgtcgc tgtgttcggg aatcgcggat tcgattgttt 2580
ataccctacc agtcgcacat cccaagaaat gcgttgtatt aatagtcatt ggggacgaca 2640
acgttgatat gcaaattcga tccgagcagg cgattagtcg gtattattac tctcgtagac 2700
atatcgcggg tgtcgtgtct atatgtatca gccaggatgg tgggttaaaa gcatactcaa 2760
ctggcatcgt caagcatcgt atctgcgaaa aatcgatcct gaaatataag tgtaaagtcg 2820
ttttagtaag aatgcccggc tatgtatttg gaaatgatga attgatgaca aaactactta 2880
acgtttaggt cccgcgacac ggacctgtgg cctcttacgc ttac 2924
<210> 128
<211> 1638
<212> DNA
<213> 人工序列
<220>
<223> BTV24 AJ586730的VP5 DNA
<400> 128
gttaaaaagt gttctcctac tcgcagaaga tggggaagat aattaaatcg ctaagtagat 60
ttggaaaaaa ggttggaaat gcattgtcgt caaacacagc gaagaaaatt tattcaacca 120
ttgggaaagc agcagagcgg tttgctgaaa gcgagatcgg tgcggcgaca atagatggtt 180
tggtgcaggg tagcgttcat tccataatta caggtgaatc ttatggagag tcagttaaac 240
aggcggttct cctcaacgtg ttaggtacag gtgaagaatt accagaccca ctgagccctg 300
gcgagcgtgg tatgcaaaca aaaataaaag aattagaaga tgagcagcga aatgaacttg 360
tccgattaaa gtataacaaa gagataacaa aagagtttgg gaaggagcta gaagaagttt 420
acgacttcat gaatggtgag gcgaaagagg aggaagtagt tcaggaacaa tactcaatgc 480
tatgcaaagc agtagattca tatgaaaaaa tattaaaagc agaggactcg aaaatggcaa 540
tattggcgcg cgcgttgcag cgggaggctt cagagaggag tcaagatgaa atcaaaatgg 600
tgaaagaata cagacagaag atcgatgcgc taaaaaatgc gatcgagatt gagcgagatg 660
gaatgcagga ggaggcgatc caggagattg ccggaatgac tgcggatgtc ttagaagcag 720
catcggagga ggtgccctta attggcgcgg gtatggccac agccgtagcg accggtagag 780
caatagaggg cgcgtataag ttgaagaaag tcataaatgc attaagcggg attgatttat 840
cgcatatgag gagcccgaag attgagccca ctattatcgc tacgacattg gaacatcgat 900
ttaaagagat accagatgaa cagttggcag taagtgtgct gaataaaaag acagctgtga 960
ccgataactg taatgaaatt gcgcatatca aacaagaaat attaccaaag tttaagcaga 1020
ttatggatga agagagagag attgaaggaa tagaggataa agtgattcat ccgcgagtga 1080
tgatgaggtt caaaattcct agaacgcagc aaccgcaaat tcacatttat gcggccccgt 1140
gggattctga tgacgtattc ttctttcatt gcgtttcaca ccatcatcgg aatgagtctt 1200
ttttcctggg attcgatttg ggaatcgatg tcgttcactt tgaagatttg actagccatt 1260
ggcacgcgtt gggattagcg caagaggcga gcgggcgtac gttaacggag gcgtatcgcg 1320
aatttctcaa tctatcaatc tcaaatacgt atagtagcgc tatacatgcg agacgtatga 1380
tcaggtcgcg ggcagtacac cctatttttc taggatcgat gcattatgat atcacgtatg 1440
aagctctaaa aaataatgcg cagagaatag tttatgatga ggagttgcag atgcatattc 1500
tgaggggacc tttgcacttc cagcgtcgag ccattctagg ggcgctgaaa tttggagtta 1560
aaatattagg cgataaaatt gatgttcccc tcttcttacg aaatgcttga acgcagcgag 1620
ggagaagctt acactcac 1638
<210> 129
<211> 2880
<212> DNA
<213> 人工序列
<220>
<223> BTV25 EU839840的VP2 DNA
<400> 129
atggatgagc tagttatacc aatattctca gcggatgaaa tgccttatgc actaataagt 60
cattatccgt tagcaataca aacaaacagg aagattcaga atgatcgtga attccatgac 120
ctgacaaaga ttccccaatc taatatgata gatatttcac aacacggcgt gattagagct 180
ctagattatc gtccttcgag gaatgatggg atagtaatac caaggttgtt ggatataaca 240
ctgcacgcgt atgataagag acggtcgatg aaggatatat gtggattaga atttaaaacg 300
gatgtgaaat ggatgcaatg ggctatcaat gatagaatgg atatacagcc attgaaagtc 360
gagatcgata atgaacatac ggtgaatcat caattattta attgtaatat aaaaatcaga 420
tctaaaaatg tcgatacggt gtactacgat tatcatccgc ttgagagtga ggcgactaag 480
tgtaatcatg cgaacataga gctaatgcgg agcttaacga tggtggaatt gtaccatgtt 540
ttacaaggtg ccgcttacgc tttaaaaacg aattttgagc tgattgcgac ggcggagaga 600
ggagacgtac gacagagata tgacgcagca ggggtaagat ggtccagact atcacgagat 660
caaaggatag ggtacagcgg caacgcttac gatatgttta tggctaacct agtgcaagtg 720
gaggtaaagg ggaagtgcgc agatgaaatt gcgggtgaga tgggacgact tgcgaaaatc 780
aaagacaagt gggtgtcaaa cgggttttct ggcactgtaa tattcgtgtc ggagttatgt 840
acagagtttt cagcaatcgg acgtaagatg atggatacac atgaagaacc gaaggatgaa 900
gagggtattt cacgacgctt tcaaattgag ttagatggca tgtttagtgc ggcgcgtcag 960
gagaataaaa acgtgctgag tcagagaggc agcaaggtaa ctgatgaatc caagttctat 1020
gctttaatca tgatagcggc gtcggacaca cagcagggaa gagtgtggag aacgaacctt 1080
tacccttgtt tgcgaggtgc gttggttgca gcagagtgtg tgatgggtga tgtatatcat 1140
acgctgcgta ccgttttcaa ctggagtgtg cggggtacgt atggcgggac agagcgaaat 1200
ctagagaaca ataggtatat cttctcccgc ataaaccttt ttgaaaccaa tttaccgagt 1260
ggtagtaaga ttgttcactg gtgttatgaa gaagtagcgc caattgaaac tacctatgat 1320
aatggatata tatgtaaatc gcaagaagga gtacagaatg atgagaaatt gtgtaaaatt 1380
gatgatgatt tgtatgcacg tatgcgcgat gaagcgataa cgggtggatg gcgacaggaa 1440
gacttcaagt tacataagat actgactaac ccgaatttac tcacgattga ttttgaaaag 1500
gaagcttaca ttacgtcgac ttcagattta atcttgccgg attattttga taagtggatt 1560
aaagcgccaa tatttaatgc acggctgcgg ataacgaatg gagtgattga aagtgaaaag 1620
aaaagcgacc cgtggaataa acgtgtagcg aacggcacga tgaagacttc agctgaatca 1680
ttgggatacg tgctgggtag attttatgat ttacggatgc aatttgtcga agatgtacta 1740
aacacccgac agcagaagtc attagctctt caacacatac gtaatctaga agattttacg 1800
agattaacag attatgggga ggaacaaaat atttgtcctc atgcgggcgg cgcaatgtat 1860
acatttcgaa gggtagctct agaaataata acaaattatg agagactgaa cccaatggat 1920
catgaaggta atgagtatca aacatatcgc catccagcta ttgattactc aaagcttggt 1980
gacgttctta agatgaaaga tttatctcaa ctcatctgtt ttgttttcga tagtatattt 2040
gagcggcgcg accaattgcg tgaggttgga gaggcgcgaa tgatcatata tcaaatccag 2100
aacgcgactg gggaacggcg acttgatgtg ttaaaacggg agtttccaac gttctacgta 2160
tggttccata ggttacggga tgttgagctt attcgtgatt taaacgttat caattttctt 2220
ccactactgt ttttagtgcg agataatgct gcataccggc atagacagtg gtcaattccc 2280
atgatacttt atgacgaaga aattaaatta atacccgtag aggttggcgc atatgcaaat 2340
agattcgggt ttaagagttt tattaatttt actcgattcc atccaggcga tgcgcgtaag 2400
agacaggaag tgagtgcgtt tcataaggct tatggttcga tttgttatcg ctactatacc 2460
gatacgacaa tatcgcaagg tgaggtaaac gtaccggtta tcactacaaa gttggacaca 2520
cttagagtgc atttagcagc attatgcggc ggtttatcgg actcgctcgt ttataccctc 2580
ccaataacac atccagagaa gtgccttgtg ttagttattg tcggagataa taagttgaat 2640
ccacagattc gatcagaact ggtaattagc agataccgct attcacgtcg acacatacgt 2700
ggtgttgtat ctgtttgtat tgaccaagag ggactgccca atgtttattc cactggtata 2760
gcgagacata aaatatacga gaaaacggtg ttaaggtata gatgtaagat aattttggta 2820
aagatatccg gatacgtgtt tggaaacgat gaacttatga ctaagctgct caatgtttag 2880
<210> 130
<211> 1581
<212> DNA
<213> 人工序列
<220>
<223> BTV25 EU839842的VP5 DNA
<400> 130
atgggaaaga tcattagatc gctaagtcgt ttcggaaaga aggtgggaag tgcgctaaca 60
tcgaatacag cgaaaaagat ttatgcgaca attggaaggg cagcagaacg tttagccgag 120
agtgagatcg gatcggcgac aatagacgga ttattacagg gtagcgtaca ctcgctcgta 180
actggagaat catacggcga gtcagtgaaa caagcagtat tattaaacgt attaggtact 240
ggagaggagt tgccggaccc tctaagtcca ggagagcgtg gattgcaagt taagctaaag 300
gagctggaag atgagcagag aaatgaccta gttcggttaa gatatggcga acagattaga 360
gccaagtttg gggccgagct ggatgagatc tatcaattta tgactagcgc gatcagaaat 420
gatggagaag cgaaggaaga gtatgagata ctaaataagg ctatcaactc atatgagaag 480
atactcaagg cagaggagac gcaaatctca accctagcac gcgcactaca gcgtgaggca 540
tctgaaagaa gcgaaaatga gattacgatg gtgaaggagt ataggcaaaa aatcgatgct 600
ttaagggatg caatagaagt tgaacgtgat ggtatgcagg aggaagcgat tcaggaaatt 660
gcagggatga cagcggatgt tcttgaggcg acatcagaag aagtgccctt ggttggggcg 720
ggcgtggcgg cgacaatcgc gacgggacgt gcaatcgagg gcgcttacaa attaaaaaag 780
gtaattagcg cgttaagtgg catcgatttg tctcatatgc gcaccccaag ggttgaaccg 840
acaacccttg caactacgtt agatcggcgg ttcggcgaga tatcggacaa agacctagcg 900
ataggtgtgc tgaacaagag gacgtcggtt cttgacaatt atagtgaaat agcacatata 960
aaacaggaaa taatgccgag gttcaagcga attctagatg aagaaaggaa gattgagggt 1020
gctaataaaa agtatataca tccaagggtt atgatgaaat ttaagattcc aaggacgcag 1080
cagccgcata ttcatatata ttctgctccg tgggattctg atgatgtttt ctttttccat 1140
tgcgtttcac atcatcaccg aaacgagtca ttctttcttg gctttgattt gggaattgat 1200
gttgttcatt ttgaagattt ggcgagccat tggcatgctc taggttcagc gcaggaggcg 1260
agtggtcgga ccctgactga ggcgtacaaa gagttcttaa atttatccat ttcaagtgcg 1320
tttagttcac caatacatgc aaagcgcatg attagatcac gaaccgtaca ccccatctat 1380
ttgggttcta tgcattatga tgtaacgtat gaagtattga aaaataacgc ccagagaatt 1440
gtatacgatg atgagctgca gatgcacata ctacggggac cactgcactt ccaaagacgc 1500
gcaatcctgg gggcactaaa gtttggcgtt aagatattag gcgagaaaat cgatatttcc 1560
ctcttcttac gaaatgcctg a 1581
<210> 131
<211> 2929
<212> DNA
<213> 人工序列
<220>
<223> BTV26 HM590642的VP2 DNA
<400> 131
gttaaaagag cgttccacca tggaggaatt tgtcattccc atatattcga gtagagacat 60
gccattagcg ctgatcagtc aatatccact cgcggtgcaa gtagacgcga ttatggcgga 120
tataggggat gatcataatt tagctttaat tccagagtta aacatgattg atatctctaa 180
gcaagggatt atcgatgcgt tggattaccg accagcaaga aacgatggag tggtggtacc 240
aaggttactg gatattacgt tacacgcata tgactgcaaa aggtcgatga ggacagcacg 300
gaatactgaa ttcgcgacgg atactgaatg gatgaagtgg gcgatagagg atagaatgga 360
cgtgcaacca ctaaaaattt taattgacga ccattatttt attaaccacc aattgtttaa 420
ttgtaacgtt aaggcaaaat ttggtaatgt tgatacaatc tattatgatt tgcacccgct 480
cgaaaagaag gaaaagaaat gtaatcatat gaacatagag ctaatgcgga gtttgactat 540
gactgaaatg ttccatgttc tgcaaggagc cgcatatacc ctaaagacga actatacatt 600
gacggcraca gccgaaagac aagacatatc ccatagcttt gaaaacgtag gtttgagatg 660
gcaaaatctg gcgccgaata cgcggattga aaatagagga tctgcatacg acaaatttat 720
tgccgggttg gtgcaagtaa ggattgacgg aaagtgccca aatgaaatca cggcagaact 780
gaaccagcta cggagtgtaa aagagaaatg ggttggggcg caatatcaag gcaacgcaat 840
atacgctcat gaaatatgta gtgtgatgtc cgcaattggc cgcaagatgc taaatgcacg 900
tgaggagcca gttgatgaag aaggtatctc tagacgtttt cagcaggaga tagacgacgt 960
attcagccag atgaatcggg aaaacgttgc cgtgcgcaga gtcgctgtcc acgcaaacga 1020
cgaacagaaa ttttttgccc tgatggttat cgcggctact gacgtaaacc aggggagaat 1080
ctggaggacg aacccatatc cgtgcttgcg aggtacgtta attgcggcgg aatgcgcgtt 1140
gggtgatgtg tattcagcaa tgcgtagaat cttcagctgg agcgtacgcc caacctatgg 1200
aacgggtgaa aagcagttgg aaaacaacag atacgtgtac agtcgcataa acctttttga 1260
gtcgaatatc tcagttgatg aggaagttat tcattggcgc tatgaattat tggctcctgc 1320
cgagaccaca tacgataggg gttacatatg ctcggtagaa cggggagatg acgatttatt 1380
gtgtaagatc gacgatgccg cctataaatt gatgtttgat gaagcagtgc gagtggggtg 1440
gcagcaacac acgtttaagc tctataagat gctaactgag ccgaatttac ttacaataga 1500
cttcgaaaaa gacgcttatt taggaccgga ttcaagctta gtgatgccgg attattataa 1560
taagtggata aggagtccaa tgtttaacgc acgactacga ttgacgaagg cgcgaattaa 1620
tgcagagaag acgggcgatc cgtggaacga tcgagtggtg ggtgggacta ttaagactcc 1680
agttgagtcg ttaggataca cgctgggaag attctatgac ttacgcttac agttcgtcga 1740
agatgttttg agttataggc agcagcaatc tgcaattttt cagtacatgg gtgcacgaga 1800
ggacttcgct atgctaacac attacgctag aggtgaaagg gatatatgcc cgcatgcggg 1860
gggaactcta tatataacgc gccgagtggc gctagaggtg ttaacaagtt atgaaagact 1920
tgatccacaa tatcaccacg gtcgtgagca tttaacttac tgtcatccat ccattgatta 1980
tacgcgtcgg gagcgcattt tcgagataga agacctggcg cagttgattt gctatgtaat 2040
agattatatt tttgaaaaac gagattcatt acgtgaggtt ggagaagcga gaagaatcgt 2100
atatctgatt cagagcgtta ctgggatacg tcgacatgaa gtacttaacc aggcgtttcc 2160
gagattcttc gcatggtttc tcagactaaa aaacgtcgag gtgattcgtg atctgaacat 2220
cgtgaacttc ttgcctctat tatttttggt tcgcgataac atatcatatc aacatcgaca 2280
gtggtcggtt ccgatgatcc tttatgatca aacgattaag ttaatacctg tagaggttgg 2340
tgcgtatgcg aatagagtag gctttaaaag ttttttcaac ttcgttcagt tccatcccgg 2400
cgacgcacaa aaacggcagg aggcggatga atcacataag aaaatgggcc agatctgtta 2460
cgcatactat actgatacta cgatttcaca gggcaaagta gatacgcccg tgattgcaac 2520
taagatggac acgttacggg tgcatctggc agcagtgtgc gcaggtctgt cagattcgct 2580
tgtttacgcg ctgccagttg cgcatcccga gagatgtatt gttttaataa ttatagggga 2640
tgaaaaactc gatccaaatg tccgcaccga attagtagtt agtagatacc gttattcacg 2700
taaacacata cgaggcgcgg tctcaatatg catgtataag aatggtcagt ttagggtgca 2760
tacgactggc atagtaagac accggatttg cgaaaaaacg atacttaact tcagatgccg 2820
tgcgatatta gtgaggacac cgggatatgt ctttggaaac gatgaattga tgacaaagtt 2880
gctcaacgtg taggtccttt gacacggacc ggtggcctct tacacttac 2929
<210> 132
<211> 1629
<212> DNA
<213> 人工序列
<220>
<223> BTV26 JN255159的VP5 DNA
<400> 132
gttaaaaagt accctctaac tcgcagaaga tggggaaaat cataaagtcg ctgagtaggt 60
tcggcaaaaa gattgggagt gcactgacat caaatgcggc aaagaaaatt tacgccaccg 120
ttgggaaagc ggcggaacgc ctcgcgacta gtgagttggg gacagcagcg attgatgggc 180
tattgcaagg cagtgtacat tcaatgataa cgggggaatc ttacggcgag tcagtgaaac 240
aggctgtttt actgaacgta ttaggtactg gggaggagct accagaccca ttgagtccgg 300
gagaacgggg aatgcagatg aaaattcaag agctcgaaga tgagcagcgt agtgaattga 360
tccgattgaa atatgatgat gaaattacac gcgcgttcgg caatgagcta cgggaggttt 420
atcaattcat gaatggtcaa gttcgtgcgg atctggatca atatgatgtg ctacgcaagg 480
cagtagattc gtacgaaaaa cttctagacg cagaagatgc gaagatggcg atattagcac 540
gtgcattaca aaaagaaatg cgggaaagga gtgccgagga agcaaagatg gttcgagagt 600
ataggagtaa aatagatgca cttaaagaag caattgaggt tgaacgtgat gggatgcaag 660
aagaggcgat tcaggaaatt gcggggatga ctgcggatgt tttggaggca acggcagagg 720
aagtcccact tataggcgca ggcgcggctg cagcgatcgc ttcagggcga gcaatcgagg 780
gggcgtataa gttgaagaaa gtgataaatg cacttagcgg tattgactta tcacatatgc 840
gtacgccgaa aatagagcct accgttatct ctacggtact tgaacatcgt tttaagagta 900
tacccgataa cctattggcg aatggcgttt taagcaagaa gatttcgatt atggataacc 960
aaaacgaaat aaggcacata aagcgagaaa tattgcctag gtttaagcgc attatggatg 1020
aggaaagagc agcggctaac agggaacata aagtaattca tccaacggta atgatgaaat 1080
tcaaggtacc aagggcgcaa cagccccaga tacatatata tgcggcgccg tgggattcgg 1140
atgaagtgtt ctttttccat tgtgtctcac atcaccatcg taatgaatct ttcttcttgg 1200
gattcgacct gggaattgat attgttcatt ttgaagatct gacgaatcat tggcacgccc 1260
taggagttgc gcaagaagct aaaggccgca ccttaggtga agcgtataaa gaatttctga 1320
atctcccaat atcaagctct tttagttcaa ggatacatgc tcgacgtatg gtcagatcac 1380
ggttagtcca cccaattttc ttaggatcga tgcattacga tgtgacctac gaaacgttga 1440
aaaacaacgc gcagagaata gtatatgatg aggagctgca aatgcatata ttgcgtggac 1500
ctctacactt ccaacgtcgc gcaatattag gagcgctgaa gtttggggtg aagatactag 1560
gcgacaaaat tgatatcccc ctcttcttaa gaaatgcctg agcgcagcgg gggaggtgct 1620
tacacttac 1629
<210> 133
<211> 961
<212> PRT
<213> 人工序列
<220>
<223> BTV1 sBTV ACR58459.1的VP2
<400> 133
Met Asp Glu Leu Gly Ile Pro Val Tyr Lys Arg Gly Phe Pro Glu His
1 5 10 15
Leu Leu Arg Gly Tyr Glu Phe Ile Ile Asp Val Gly Thr Lys Ile Glu
20 25 30
Ser Val Gly Gly Arg His Asp Val Thr Lys Ile Pro Glu Met Asn Ala
35 40 45
Tyr Asp Ile Lys Gln Glu Ser Ile Arg Thr Ala Leu Trp Tyr Asn Pro
50 55 60
Ile Arg Asn Asp Gly Phe Val Leu Pro Arg Val Leu Asp Ile Thr Leu
65 70 75 80
Arg Gly Tyr Asp Glu Arg Arg Ala Val Val Glu Ser Thr Arg His Lys
85 90 95
Ser Phe His Thr Asn Asp Gln Trp Val Gln Trp Met Met Lys Asp Ser
100 105 110
Met Asp Ala Gln Pro Leu Lys Val Gly Leu Asp Asp Gln Ser Arg Asn
115 120 125
Val Ala His Ser Leu His Asn Cys Val Val Lys Ile Asp Ser Lys Lys
130 135 140
Ala Asp Thr Met Ser Tyr His Val Glu Pro Ile Glu Asp Ala Ser Lys
145 150 155 160
Gly Cys Leu His Thr Arg Thr Met Met Trp Asn His Leu Val Arg Ile
165 170 175
Glu Thr Phe His Ala Ala Gln Glu Val Ala Tyr Thr Leu Lys Pro Thr
180 185 190
Tyr Asp Ile Val Val His Ala Glu Arg Arg Asp Arg Ser Gln Pro Phe
195 200 205
Arg Pro Gly Asp Gln Thr Leu Ile Asn Phe Gly Arg Gly Gln Lys Val
210 215 220
Ala Met Asn His Asn Ser Tyr Asp Lys Met Val Glu Gly Leu Thr His
225 230 235 240
Leu Val Ile Arg Gly Lys Thr Pro Glu Val Ile Arg Asp Asp Ile Ala
245 250 255
Ser Leu Asp Glu Ile Cys Asn Arg Trp Ile Gln Ser Arg His Asp Pro
260 265 270
Gly Glu Ile Lys Ala Tyr Glu Leu Cys Lys Ile Leu Ser Thr Ile Gly
275 280 285
Arg Lys Val Leu Asp Arg Glu Lys Glu Pro Glu Asp Glu Ala Asn Leu
290 295 300
Ser Ile Arg Phe Gln Glu Ala Ile Asp Asn Lys Phe Arg Gln His Asp
305 310 315 320
Pro Glu Arg Leu Lys Ile Phe Glu His Arg Asn Gln Arg Arg Asp Glu
325 330 335
Asp Arg Phe Tyr Ile Leu Leu Met Ile Ala Ala Ser Asp Thr Phe Asn
340 345 350
Thr Arg Val Trp Trp Ser Asn Pro Tyr Pro Cys Leu Arg Gly Thr Leu
355 360 365
Ile Ala Ser Glu Thr Lys Leu Gly Asp Val Tyr Ser Met Met Arg Ser
370 375 380
Trp Tyr Asp Trp Ser Val Arg Pro Thr Tyr Thr Pro Tyr Glu Lys Thr
385 390 395 400
Arg Glu Gln Glu Lys Tyr Ile Tyr Gly Arg Val Asn Leu Phe Asp Phe
405 410 415
Val Ala Glu Pro Gly Ile Lys Ile Val His Trp Glu Tyr Arg Leu Asn
420 425 430
His Ser Thr Arg Glu Ile Thr Tyr Ala Gln Gly Asn Pro Cys Asp Leu
435 440 445
Tyr Pro Glu Asp Asp Asp Val Ile Val Thr Lys Phe Asp Asp Val Ala
450 455 460
Tyr Gly Gln Met Ile Asn Glu Met Ile Asn Gly Gly Trp Asn Gln Glu
465 470 475 480
Gln Phe Lys Met His Lys Ile Leu Lys Ser Glu Gly Asn Val Leu Thr
485 490 495
Ile Asp Phe Glu Lys Asp Ala Lys Leu Thr Thr Asn Glu Gly Val Thr
500 505 510
Met Pro Glu Tyr Phe Asn Lys Trp Ile Ile Ala Pro Met Phe Asn Ala
515 520 525
Lys Leu Arg Ile Lys His Glu Glu Ile Ala Gln Arg Gln Ser Asp Asp
530 535 540
Pro Met Val Lys Arg Thr Leu Ser Pro Ile Thr Ala Asp Pro Ile Glu
545 550 555 560
Leu Gln Arg Leu Thr Leu Ala Arg Phe Tyr Asp Ile Arg Pro Ala Leu
565 570 575
Arg Gly Gln Ala Leu Ser Arg Gln Gln Ala Gln Ser Thr Tyr Asp Glu
580 585 590
Glu Ile Ser Lys Gln Arg Asp Tyr Ala Glu Ile Leu Lys Arg Arg Gly
595 600 605
Ile Val Gln Ile Pro Lys Lys Pro Cys Pro Thr Val Thr Ala Gln Tyr
610 615 620
Thr Leu Glu Arg Tyr Ala Leu Phe Ile Ile Asn Ile Leu Gln Gln His
625 630 635 640
Val Ala Arg Asp Cys Asp Glu Glu Ala Ile Tyr Glu His Pro Lys Ala
645 650 655
Asp Tyr Glu Leu Glu Ile Phe Gly Glu Ser Ile Val Asp Ile Ser Gln
660 665 670
Val Ile Val Leu Val Phe Asp Leu Ile Phe Glu Arg Arg Arg Arg Val
675 680 685
Arg Asp Val Tyr Glu Ser Arg Tyr Ile Ile Ala Arg Ile Arg Arg Met
690 695 700
Arg Gly Lys Glu Arg Leu Asn Val Ile Ala Glu Phe Phe Pro Thr Tyr
705 710 715 720
Gly Ser Leu Leu Asn Gly Leu Asn Ser Ala Thr Val Val Gln Asp Ile
725 730 735
Met Tyr Leu Asn Phe Leu Pro Leu Tyr Phe Leu Ala Gly Asp Asn Met
740 745 750
Ile Tyr Ser His Arg Gln Trp Ser Ile Pro Leu Leu Leu Tyr Thr His
755 760 765
Glu Val Met Val Val Pro Leu Glu Val Gly Ser Tyr Asn Asp Arg Cys
770 775 780
Gly Leu Ile Ala Tyr Leu Glu Tyr Met Val Phe Phe Pro Ser Lys Ala
785 790 795 800
Ile Arg Leu Ser Lys Leu Asn Glu Ala Gln Pro Lys Ile Ala Arg Glu
805 810 815
Met Leu Lys Tyr Tyr Ala Asn Thr Ala Val Tyr Asp Gly Gly Val Asn
820 825 830
Tyr Asn Val Val Thr Thr Lys Gln Leu Leu Tyr Glu Thr Tyr Leu Ala
835 840 845
Ser Leu Cys Gly Gly Ile Ser Asp Gly Ile Val Trp Tyr Leu Pro Ile
850 855 860
Thr His Pro Asn Lys Cys Ile Val Ala Ile Glu Val Ser Asp Glu Arg
865 870 875 880
Val Pro Ala Ser Ile Arg Ala Gly Arg Ile Arg Leu Arg Phe Pro Leu
885 890 895
Ser Ala Arg His Leu Lys Gly Val Val Ile Ile Gln Ile Asp Glu Glu
900 905 910
Gly Glu Phe Thr Val Tyr Ser Glu Gly Ile Val Ser His Arg Val Cys
915 920 925
Lys Lys Asn Leu Leu Lys Tyr Met Cys Asp Ile Ile Leu Leu Lys Phe
930 935 940
Ser Gly His Val Phe Gly Asn Asp Glu Met Leu Thr Lys Leu Leu Asn
945 950 955 960
Val
<210> 134
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV1 sBTV ACR58462.1的VP5
<400> 134
Met Gly Lys Val Ile Arg Ser Leu Asn Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ser
65 70 75 80
Gly Glu Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Ala Lys Leu Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Asp Lys Ile Lys Glu Lys Phe Gly Lys Glu Leu Glu
115 120 125
Glu Val Tyr Asn Phe Met Asn Gly Glu Ala Asn Ala Glu Ile Glu Asp
130 135 140
Glu Lys Gln Phe Asp Ile Leu Asn Lys Ala Val Thr Ser Tyr Asn Lys
145 150 155 160
Ile Leu Thr Glu Glu Asp Leu Gln Met Arg Arg Leu Ala Thr Ala Leu
165 170 175
Gln Lys Glu Ile Gly Glu Arg Thr His Ala Glu Thr Val Met Val Lys
180 185 190
Glu Tyr Arg Asp Lys Ile Asp Ala Leu Lys Asn Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Thr His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Ser Val Val Ser Thr Ile Leu Glu
275 280 285
Tyr Arg Ala Lys Glu Ile Pro Asp Asn Ala Leu Ala Val Ser Val Leu
290 295 300
Ser Lys Asn Arg Ala Ile Gln Glu Asn His Lys Glu Leu Met His Ile
305 310 315 320
Lys Asn Glu Ile Leu Pro Arg Phe Lys Lys Ala Met Asp Glu Glu Lys
325 330 335
Glu Ile Cys Gly Ile Glu Asp Lys Val Ile His Pro Lys Val Met Met
340 345 350
Lys Phe Lys Ile Pro Arg Ala Gln Gln Pro Gln Ile His Val Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Ile Ser His
370 375 380
His His Ala Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Ser Ile Asp
385 390 395 400
Leu Val His Tyr Glu Asp Leu Thr Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Thr Ala Ala Gly Arg Thr Leu Thr Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ala Ile Ser Asn Ala Phe Gly Thr Gln Met His Thr Arg
435 440 445
Arg Leu Val Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Leu
450 455 460
His Tyr Asp Ile Ser Phe Ser Asp Leu Arg Gly Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Ile His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Cys Lys Val
500 505 510
Leu Gly Asp Arg Leu Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 135
<211> 962
<212> PRT
<213> 人工序列
<220>
<223> BTV2 sBTV AEO19855.1的VP2
<400> 135
Met Asp Glu Leu Gly Ile Pro Ile Tyr Gly Arg Asn Tyr Pro Glu His
1 5 10 15
Leu Leu Lys Gly Tyr Glu Phe Leu Ile Asn Thr Gly Val Arg Tyr Pro
20 25 30
Ser Gln Gly Gly Arg His Asp Val Ser Lys Ile Pro Glu Met Phe Ala
35 40 45
Tyr Asp Ile Lys Asp Glu Gly Ile Arg Glu Ala Leu Lys Tyr Met Pro
50 55 60
Thr Arg Asn Asp Gly Val Val Leu Pro Arg Ile Val Asp Ile Ser Leu
65 70 75 80
Lys Gly Tyr Asp Ile Arg Lys Ser Val Ile Glu Ala Asn Lys Ser Asn
85 90 95
Ser Phe His Thr Asp Thr Asn Trp Val Gln Trp Met Ile Lys Asp Ser
100 105 110
Met Asp Gln Gln Pro Leu Lys Ile Ser Ile Asp Glu Glu His Ser Arg
115 120 125
Val Val His Ser Leu Phe Asn Cys Gln Val Lys Ile Asp Ala Lys Lys
130 135 140
Ala Asp Thr Leu Ser Tyr His Leu Glu Ala Ile Glu Asp Ala Glu Lys
145 150 155 160
Ala Cys Leu His Thr Arg Gly Gln Leu Cys Asn His Leu Ala Arg Met
165 170 175
Asp Leu Leu His Ala Ala Gln Glu Ile Ala Tyr Ala Ile Lys Pro Thr
180 185 190
Tyr Gln Leu Ile Val His Ser Glu Arg Ala Ser Thr Ser Asp Asn Phe
195 200 205
Glu Leu Gly Arg Gln Asp Val Ile Thr Leu Arg Arg Gly His Arg Ile
210 215 220
Gln Met Gly Asp Glu Ala Tyr Thr Lys Leu Met Glu Arg Leu Val Arg
225 230 235 240
Leu Thr Val Gln Gly Asn Val Pro Arg Lys Ile Gln Ser Glu Ile Glu
245 250 255
Gln Leu Glu Ala Ile Arg Thr Arg Trp Ala Thr Gly Arg Tyr Asp Pro
260 265 270
Ala His Ile Asn Ser Gln Asp Leu Cys Arg Ile Leu Ser Arg Ile Gly
275 280 285
Arg Ile Met Leu Asp Gln Glu Ala Glu Pro Val Asp Glu Asp Ser Leu
290 295 300
Ser Leu Arg Phe Gln Arg Ala Leu Asp Glu Lys Phe Arg Leu Asn Asp
305 310 315 320
Ser Glu Arg Asn Lys Ile Phe Glu Pro Lys Ser His Arg Lys Asp Glu
325 330 335
Asp Arg Phe Tyr Val Leu Leu Ala Ile Ala Ala Ser Asp Thr Tyr Asn
340 345 350
Ser Arg Ile Trp Trp Ser Asn Pro Tyr Pro Cys Leu Arg Gly Thr Leu
355 360 365
Ile Ala Ala Glu Thr Lys Leu Gly Asp Val Tyr Phe Thr Leu Arg Ser
370 375 380
Trp Tyr Asp Trp Ser Val Arg Ser Ser Tyr Val Pro Arg Glu Arg Glu
385 390 395 400
Arg Glu Thr Glu Lys Tyr Ile Phe Ser Lys Ile Asn Leu Phe Asp Tyr
405 410 415
Glu Ala Gly Pro Ser Ser Lys Val Ile His Trp Glu Tyr Gln Leu Tyr
420 425 430
Lys Arg Glu Arg Val Val Thr Leu Glu Arg Gly Asn Pro Cys Asp Leu
435 440 445
Tyr Pro Asp Glu Asp Asp Glu Val Ile Ile Thr Lys Phe Asp Asp Ala
450 455 460
Lys Tyr Ser Glu Met Val Gly Glu Ile Ile Asp Gly Gly Trp Asn Asp
465 470 475 480
Glu Glu Phe Lys Met Tyr Lys Leu Leu Gln Glu Lys Gly Asn Val Leu
485 490 495
Thr Ile Asp Phe Glu Lys Asp Thr Lys Leu Asn Ser Thr Ser Glu Val
500 505 510
Val Leu Pro Asp Tyr Tyr Gly Lys Trp Ile Val Ala Pro Met Phe Asn
515 520 525
Ser Lys Met Arg Ile Ile Glu Thr Glu Ile Ala Thr Asn Arg Ser Asp
530 535 540
Asp Pro Met Ile Lys Arg Thr Leu Lys Pro Met Thr Asp Asp Pro Val
545 550 555 560
Glu Leu Gln Arg Tyr Thr Leu Ala Arg Tyr Tyr Asp Ile Arg Pro Gly
565 570 575
Leu Met Gly Arg Ser Leu Asn Arg Thr Gln Thr Gln Ser Thr Phe Asp
580 585 590
Ala Lys Val Ser Glu Leu Ser Asp Tyr Glu Lys Val Val Ser Arg Phe
595 600 605
Gly Val Ile Lys Lys Pro Thr Arg Pro Cys Val Thr Leu Thr Gly Arg
610 615 620
Tyr Ile Leu Glu Lys Tyr Ser Leu Leu Leu Ile Asp Ile Leu Lys Tyr
625 630 635 640
His Thr Glu Val Glu Glu Asn Pro Gln Glu Glu Phe Thr His Pro Arg
645 650 655
Ile Asp Ser Gln Phe Lys Phe Asn Gly Asn Thr Leu Ser Asp Leu Asn
660 665 670
Gln Thr Val Val Phe Ile Val Asp Tyr Leu His Glu Lys Arg Asn Tyr
675 680 685
Val Arg Ser Val Tyr Glu Ala Arg Tyr Ile Ile Ser Arg Ile Arg Ser
690 695 700
Ser Thr Gly Ala Ala Arg Met Ser Ile Leu Glu Phe Tyr Phe Pro Thr
705 710 715 720
Phe Ala Arg Leu Ile Ser Asn Ala Arg Glu Pro Thr Tyr Val Lys Asp
725 730 735
Leu Met Ala Leu Asn Phe Leu Pro Leu Leu Phe Ile Val Gly Asp Asn
740 745 750
Met Ile Tyr Lys His Arg Gln Trp Ser Ile Pro Leu Leu Leu Tyr Thr
755 760 765
Asp Arg Val Lys Val Ile Pro Leu Glu Val Gly Ser Ser Asn Asn Arg
770 775 780
Gln Gly Leu Val Ser Tyr Leu Glu Tyr Met Phe Phe Phe Pro Ser Leu
785 790 795 800
Ala Asp Arg Thr Ser Lys Val Asp Glu Ser Met Ile Lys Val Ser Lys
805 810 815
Glu Val Val Asn Tyr Tyr Met Lys Thr Thr Ile Ser Glu Gly Gly Val
820 825 830
Asn Leu Asn Val Val Ser Thr Lys Ser Leu Leu Tyr Asp Ile Tyr Leu
835 840 845
Ser Ser Val Cys Gly Gly Val Ser Asp Gly Val Val Trp Tyr Leu Pro
850 855 860
Ile Thr His Pro Tyr Lys Cys Val Val Ala Ile Glu Val Cys Asp Asp
865 870 875 880
Arg Val Pro Ala Arg Leu Arg Cys Asp Arg Leu Arg Leu Arg Phe Pro
885 890 895
Leu Ser Ala Gln His Leu Lys Gly Ile Val Val Ile Gln Ile Asn Glu
900 905 910
Glu Gly Gly Phe Asp Val Tyr Thr Glu Gly Ile Val Thr His Arg Val
915 920 925
Cys Lys Lys Ser Leu Leu Lys His Val Cys Asp Ile Val Leu Leu Lys
930 935 940
Phe His Gly His Val Phe Gly Asn Asp Glu Met Leu Thr Lys Leu Leu
945 950 955 960
Asn Val
<210> 136
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV2 sBTV AEO19851.1的VP5
<400> 136
Met Gly Lys Val Ile Arg Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Ser Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Leu Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ser
65 70 75 80
Gly Glu Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Gln Gly Ile Gln
85 90 95
Thr Lys Leu Lys Glu Leu Glu Glu Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Asp Lys Ile Lys Glu Lys Phe Gly Glu Glu Leu Glu
115 120 125
Gln Val Tyr Glu Phe Met Ser Gly Ala Ala Lys Glu Glu Ala Gly Asp
130 135 140
Glu Lys Gln Phe Asp Ile Leu Ser Lys Ala Val Asn Ser Tyr Asn Lys
145 150 155 160
Ile Leu Thr Glu Glu Asp Leu Gln Met Arg Arg Leu Ala Ile Ala Leu
165 170 175
Gln Lys Glu Ile Gly Glu Arg Thr His Ala Glu Thr Thr Met Val Lys
180 185 190
Glu Tyr Arg Asn Lys Ile Asp Ala Leu Lys Ser Ala Ile Glu Ile Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Thr His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Ser Val Val Ser Thr Ile Leu Glu
275 280 285
Tyr Arg Ala Lys Asp Ile Pro Asp Asn Ala Leu Ala Val Ser Val Leu
290 295 300
Ser Lys Asn Arg Ala Ile Gln Glu Asn His Lys Glu Leu Met His Ile
305 310 315 320
Lys Asp Glu Ile Leu Pro Arg Phe Lys Lys Ala Met Asp Glu Glu Lys
325 330 335
Glu Ile Cys Gly Ile Glu Asp Lys Val Ile His Pro Lys Val Met Met
340 345 350
Lys Phe Lys Ile Pro Arg Ser Gln Gln Pro Gln Ile His Ile Tyr Thr
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Ile Ser His
370 375 380
His His Ala Asn Glu Ser Phe Phe Ile Gly Phe Asp Leu Ser Ile Asp
385 390 395 400
Leu Val His Tyr Glu Asp Leu Thr Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Met Ala Val Gly Arg Thr Leu Thr Glu Ala Tyr Lys Glu Phe
420 425 430
Leu Asn Leu Ala Ile Ser Ser Thr Tyr Gly Thr Gln Met His Thr Arg
435 440 445
Arg Leu Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Leu
450 455 460
His Tyr Asp Ile Ser Phe Ala Asp Leu Arg Gly Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Cys Lys Ile
500 505 510
Leu Gly Asp Arg Leu Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 137
<211> 962
<212> PRT
<213> 人工序列
<220>
<223> BTV2 sBTV CAE51089.1的VP2
<400> 137
Met Asp Glu Leu Gly Ile Pro Ile Tyr Gly Arg Asn Tyr Pro Glu His
1 5 10 15
Leu Leu Lys Gly Tyr Glu Phe Leu Ile Asn Thr Gly Val Lys Tyr Pro
20 25 30
Ser Gln Gly Gly Arg His Asp Val Ser Lys Ile Pro Glu Met Phe Ala
35 40 45
Tyr Asp Ile Lys Asp Glu Gly Ile Arg Asp Ala Leu Lys Tyr Met Pro
50 55 60
Thr Arg Asn Asp Gly Val Val Leu Pro Arg Ile Val Asp Ile Ser Leu
65 70 75 80
Lys Gly Tyr Asp Ile Arg Lys Ser Val Ile Glu Ala Asn Lys Ser Asn
85 90 95
Ser Phe His Thr Asp Thr Asn Trp Val Gln Trp Met Ile Lys Asp Ser
100 105 110
Met Asp Gln Gln Pro Leu Lys Ile Ser Ile Asp Glu Glu His Ser Arg
115 120 125
Val Val His Ser Leu Phe Asn Cys Gln Val Lys Ile Asp Ala Lys Lys
130 135 140
Ala Asp Thr Leu Ser Tyr His Leu Glu Ala Ile Glu Asp Ala Glu Lys
145 150 155 160
Ala Cys Leu His Thr Arg Gly Gln Leu Cys Asn His Leu Thr Arg Met
165 170 175
Asp Leu Leu His Ala Ala Gln Glu Ile Ala Tyr Ala Ile Lys Pro Thr
180 185 190
Tyr Gln Leu Ile Val His Ser Glu Arg Ala Ser Thr Ser Asp Asn Phe
195 200 205
Glu Leu Gly Arg Gln Asp Val Ile Thr Leu Arg Arg Gly His Arg Val
210 215 220
Gln Met Gly Asp Glu Ala Tyr Thr Lys Leu Met Glu Arg Leu Val Arg
225 230 235 240
Leu Thr Val Gln Gly Asn Val Pro Arg Lys Ile Gln Ser Glu Ile Glu
245 250 255
Gln Leu Glu Ala Ile Arg Thr Arg Trp Ala Thr Gly Arg Tyr Asp Pro
260 265 270
Ala His Ile Asn Ser Gln Asp Leu Cys Arg Ile Leu Ser Arg Ile Gly
275 280 285
Arg Ile Met Leu Asp Gln Glu Ala Glu Pro Val Asp Glu Asp Ser Leu
290 295 300
Ser Leu Arg Phe Gln Arg Ala Leu Asp Glu Lys Phe Arg Leu Asn Asp
305 310 315 320
Ser Glu Arg Asn Lys Ile Phe Glu His Lys Ser His Arg Lys Asp Glu
325 330 335
Asp Arg Phe Tyr Val Leu Leu Ala Ile Ala Ala Ser Asp Thr Tyr Asn
340 345 350
Ser Arg Ile Trp Trp Ser Asn Pro Tyr Pro Cys Leu Arg Gly Thr Leu
355 360 365
Ile Ala Ala Glu Thr Lys Leu Gly Asp Val Tyr Phe Thr Leu Arg Ser
370 375 380
Trp Tyr Asp Trp Ser Val Arg Ser Ser Tyr Ile Pro Arg Glu Arg Glu
385 390 395 400
Arg Glu Thr Glu Lys Tyr Ile Phe Ser Lys Ile Asn Leu Phe Asp Tyr
405 410 415
Glu Ala Gly Pro Ser Ser Lys Val Ile His Trp Glu Tyr Gln Leu Tyr
420 425 430
Lys Arg Glu Arg Val Val Thr Leu Glu Arg Gly Asn Pro Cys Asp Leu
435 440 445
Tyr Pro Asp Glu Asp Asp Glu Val Ile Ile Thr Lys Phe Asp Asp Ala
450 455 460
Lys Tyr Ser Glu Met Val Gly Glu Ile Ile Asp Gly Gly Trp Asn Asp
465 470 475 480
Glu Glu Phe Lys Met Tyr Lys Leu Leu Gln Glu Lys Gly Asn Val Leu
485 490 495
Thr Ile Asp Phe Glu Lys Asp Thr Lys Leu Asn Asn Thr Ser Glu Val
500 505 510
Val Leu Pro Asp Tyr Tyr Gly Lys Trp Ile Val Ala Pro Met Phe Asn
515 520 525
Ser Lys Met Arg Ile Ile Glu Thr Glu Ile Ala Thr Asn Lys Ser Asp
530 535 540
Asp Pro Met Ile Lys Arg Thr Leu Lys Pro Met Thr Asp Asp Pro Val
545 550 555 560
Glu Leu Gln Arg Tyr Thr Leu Ala Arg Tyr Tyr Asp Ile Arg Pro Gly
565 570 575
Leu Met Gly Arg Ser Leu Asn Arg Thr Gln Thr Gln Ser Thr Phe Asp
580 585 590
Pro Lys Val Lys Lys Leu Pro Asp Tyr Lys Lys Val Val Ser Arg Phe
595 600 605
Gly Val Ile Lys Lys Pro Thr Arg Pro Cys Val Thr Leu Thr Gly Arg
610 615 620
Tyr Ile Leu Glu Lys Tyr Ser Leu Leu Leu Ile Asp Ile Leu Lys Tyr
625 630 635 640
His Thr Glu Val Glu Gly Asn Pro Gln Glu Glu Phe Thr His Pro Arg
645 650 655
Ile Asp Pro Gln Phe Lys Phe Asn Gly Asn Thr Leu Ser Asp Leu Asn
660 665 670
Gln Thr Val Val Phe Ile Val Asp Tyr Leu His Glu Lys Arg Asn Tyr
675 680 685
Val Arg Ser Ile Tyr Glu Ala Arg Tyr Ile Ile Ser Arg Ile Arg Ser
690 695 700
Ser Thr Gly Ala Ala Arg Met Ser Ile Ile Glu Phe Tyr Phe Pro Thr
705 710 715 720
Phe Ala Arg Leu Ile Ser Asn Ala Arg Glu Pro Thr Tyr Val Lys Asp
725 730 735
Leu Met Ala Leu Asn Phe Leu Pro Leu Leu Phe Ile Val Gly Asp Asn
740 745 750
Met Ile Tyr Lys His Arg Gln Trp Ser Ile Pro Leu Leu Leu Tyr Thr
755 760 765
Asp Arg Val Lys Val Ile Pro Leu Glu Val Gly Ser Ser Asn Asn Arg
770 775 780
Gln Gly Phe Val Ser Tyr Leu Glu Tyr Met Phe Phe Phe Pro Ser Leu
785 790 795 800
Ala Asp Arg Thr Ser Lys Val Asp Glu Ser Met Ile Lys Val Ser Lys
805 810 815
Glu Met Val Asn Tyr Tyr Met Lys Thr Thr Ile Ser Glu Gly Ala Val
820 825 830
Asn Leu Asn Val Val Ser Thr Lys Ser Leu Leu Tyr Glu Ile Tyr Leu
835 840 845
Ser Ser Val Cys Gly Gly Val Ser Asp Gly Val Val Trp Tyr Leu Pro
850 855 860
Ile Thr His Pro Tyr Lys Cys Val Val Ala Ile Glu Val Cys Asp Asp
865 870 875 880
Arg Val Pro Ala Arg Leu Arg Cys Asp Arg Leu Lys Leu Arg Phe Pro
885 890 895
Leu Ser Ala Gln His Leu Lys Gly Ile Val Val Ile Gln Ile Asn Glu
900 905 910
Glu Gly Gly Phe Asp Val Tyr Thr Glu Gly Ile Val Thr His Arg Val
915 920 925
Cys Lys Lys Ser Leu Leu Lys His Val Cys Asp Ile Val Leu Leu Lys
930 935 940
Phe His Gly His Val Phe Gly Asn Asp Glu Met Leu Thr Lys Leu Leu
945 950 955 960
Asn Val
<210> 138
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV2 AEO19851.1的VP5
<400> 138
Met Gly Lys Val Ile Arg Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Ser Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Leu Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ser
65 70 75 80
Gly Glu Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Gln Gly Ile Gln
85 90 95
Thr Lys Leu Lys Glu Leu Glu Glu Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Asp Lys Ile Lys Glu Lys Phe Gly Glu Glu Leu Glu
115 120 125
Gln Val Tyr Glu Phe Met Ser Gly Ala Ala Lys Glu Glu Ala Gly Asp
130 135 140
Glu Lys Gln Phe Asp Ile Leu Ser Lys Ala Val Asn Ser Tyr Asn Lys
145 150 155 160
Ile Leu Thr Glu Glu Asp Leu Gln Met Arg Arg Leu Ala Ile Ala Leu
165 170 175
Gln Lys Glu Ile Gly Glu Arg Thr His Ala Glu Thr Thr Met Val Lys
180 185 190
Glu Tyr Arg Asn Lys Ile Asp Ala Leu Lys Ser Ala Ile Glu Ile Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Thr His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Ser Val Val Ser Thr Ile Leu Glu
275 280 285
Tyr Arg Ala Lys Asp Ile Pro Asp Asn Ala Leu Ala Val Ser Val Leu
290 295 300
Ser Lys Asn Arg Ala Ile Gln Glu Asn His Lys Glu Leu Met His Ile
305 310 315 320
Lys Asp Glu Ile Leu Pro Arg Phe Lys Lys Ala Met Asp Glu Glu Lys
325 330 335
Glu Ile Cys Gly Ile Glu Asp Lys Val Ile His Pro Lys Val Met Met
340 345 350
Lys Phe Lys Ile Pro Arg Ser Gln Gln Pro Gln Ile His Ile Tyr Thr
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Ile Ser His
370 375 380
His His Ala Asn Glu Ser Phe Phe Ile Gly Phe Asp Leu Ser Ile Asp
385 390 395 400
Leu Val His Tyr Glu Asp Leu Thr Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Met Ala Val Gly Arg Thr Leu Thr Glu Ala Tyr Lys Glu Phe
420 425 430
Leu Asn Leu Ala Ile Ser Ser Thr Tyr Gly Thr Gln Met His Thr Arg
435 440 445
Arg Leu Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Leu
450 455 460
His Tyr Asp Ile Ser Phe Ala Asp Leu Arg Gly Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Cys Lys Ile
500 505 510
Leu Gly Asp Arg Leu Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 139
<211> 959
<212> PRT
<213> 人工序列
<220>
<223> BTV3 CAE51090.1的VP2
<400> 139
Met Glu Glu Leu Val Ile Pro Val Ile Ser Arg Gln Phe Asp Lys Lys
1 5 10 15
Leu Val Gly Arg Tyr Glu Tyr Val Ile Glu Leu Ala Glu Pro Glu Gln
20 25 30
Asp Glu Trp Thr Asn His Asp Val Thr Gln Ile Pro Gly Arg Arg Met
35 40 45
Phe Asp Val Ala Gln Gln Gly Ile Arg Glu Ala Ile Ile Tyr Lys Pro
50 55 60
Leu Asp Asn Asp Gly Glu Val Leu Pro Arg Ile Leu Asp Met Ser Ile
65 70 75 80
Ala Cys Tyr Asp Met Arg Lys Thr Met Met Lys Lys Glu Gly Val Asp
85 90 95
Phe Val Ser Asn Thr Arg Trp Leu Glu Trp Met Ile Gln Asp Ser Met
100 105 110
Asp Val Gln Pro Leu Arg Val Gln Met Lys Glu Asp His Ser Thr Val
115 120 125
Gln Tyr Asp Met Phe Ser Ala Lys Val His Ile Asp Ser Arg Lys Ala
130 135 140
Asp Thr Thr Ser Tyr His Ala Ile Ala Val Glu Thr Lys Ala Glu Arg
145 150 155 160
Lys Cys Cys His Val Arg Thr Glu Val Trp Asn Ser Val Val Arg Asn
165 170 175
His Leu Phe Asn Thr Ala Gln Glu Ser Cys Tyr Thr Phe Lys Gln Thr
180 185 190
Tyr Glu Leu Ile Val Asn Ser Glu Arg Leu Ser Thr Glu Glu Glu Phe
195 200 205
Arg Val Gly Ala Pro Gln Phe His Thr Ile Gln Arg Asn His Arg Met
210 215 220
Gln Leu Gly Asp Asn Ala Tyr Asp Lys Phe Leu Lys Gly Leu Val Gln
225 230 235 240
Leu Arg Val Ser Gly Thr Thr Pro Ala Lys Ile Arg Asp Glu Val Ala
245 250 255
Ala Leu Asp Val Ile Arg Asp Asn Trp Ile Arg Gly Ser Phe Asp Arg
260 265 270
Ser His Ile Lys Ser Leu Glu Leu Cys Arg Leu Leu Ser Ser Ile Gly
275 280 285
Arg Lys Met Val Asn Met Glu Glu Glu Pro Lys Asp Glu Lys Asp Leu
290 295 300
Ser Val Lys Phe Gln Phe Arg Leu Asp Glu Lys Phe Ser Pro Asn Asp
305 310 315 320
Pro Glu Arg Asn Val Ile Phe Thr Ser Lys Thr His Arg Thr Asn Glu
325 330 335
Asp Arg Phe Tyr Val Leu Leu Val Ile Ala Ala Ser Asp Thr Asn Asn
340 345 350
Gly Arg Val Trp Trp Ser Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu
355 360 365
Ile Ala Ala Glu Cys Lys Leu Gly Asp Val Tyr His Thr Leu Arg Ser
370 375 380
Lys Tyr Glu Trp Gly Val Arg Pro Thr Tyr Lys Pro Lys Asp Leu Glu
385 390 395 400
Arg Glu Arg Glu Lys Tyr Val Val Gly Arg Val Asn Leu Phe Asp Leu
405 410 415
Glu Gly Glu Pro Ala Thr Lys Val Ile His Trp Glu Tyr Glu Leu Ile
420 425 430
Ser Pro Thr Tyr Ser Val Ser Asn His Lys Gly Asn His Cys Asp Leu
435 440 445
Tyr Pro Asp Asp Val Glu Ile Thr Thr Lys Phe Asn Glu Asp Arg Tyr
450 455 460
Arg Glu Met Ile Gln Ser Val Ile Asp Asp Gly Trp Asp Gln Lys Asn
465 470 475 480
Leu Lys Met Tyr Lys Ile Leu Glu Glu Glu Gly Asn Pro Leu Leu Tyr
485 490 495
Asp Leu Glu Lys Asp Ile Lys Leu Asp Ser Gln Ser Gln Val Val Phe
500 505 510
Pro Ser Tyr Tyr Asn Lys Trp Thr His Ala Pro Met Phe Asn Ala Arg
515 520 525
Val Lys Pro Cys Asp Ile Glu Leu Ala Glu Arg Lys Asn Asp Asp Pro
530 535 540
Phe Val Lys Arg Thr Leu Lys Pro Ile Lys Ala Asp Cys Val Asp Leu
545 550 555 560
Leu Arg Tyr His Met Ser His Tyr Tyr Asp Leu Arg Pro Ser Leu Lys
565 570 575
Gly Val Ser Leu Ser Asn Lys Gln Thr Pro Ser Gly Ile His Gln Ala
580 585 590
Leu Val Gln Asp Asp Leu Tyr Ser Arg Leu Leu Arg Arg Arg Asp Val
595 600 605
Asp Leu Asp Tyr Ser Ser Pro Cys Pro Ile Ile Thr Asn Tyr Phe Leu
610 615 620
Leu Glu Lys Phe His Ile Leu Ile Leu Thr Ile Met Glu Lys His Tyr
625 630 635 640
Trp Glu Leu Asp Asp Ser Asp Asp Val Tyr Glu Phe Pro Lys Ile Asp
645 650 655
Ala Ser Ala Phe Glu Val Asp Gly Thr Leu Tyr Asp Ile Ser Gln Thr
660 665 670
Ile Val His Met Tyr Asp Arg Phe Phe Glu Lys Arg Arg Val Leu Arg
675 680 685
Ser Ile Asp Glu Ser Arg Trp Ile Leu His Leu Ile Arg Ile Ser Gln
690 695 700
Gly Arg Glu Arg Leu Glu Val Ile Glu Arg Phe Phe Pro Asn Tyr Gly
705 710 715 720
Lys Ala Met Arg Gln Arg Asp Phe Lys Lys Val Arg Asp Val Met Phe
725 730 735
Leu Asn Phe Leu Pro Phe Phe Phe Leu Thr Gly Asp Asn Ile Ser Tyr
740 745 750
Glu His Arg Gln Trp Ser Ile Pro Ile Ile Leu Tyr Ala Asp Lys Leu
755 760 765
Arg Ile Leu Pro Ile Glu Val Gly Ala His Tyr Asn Arg Phe Gly Val
770 775 780
Thr Cys Ile Leu Glu Leu Leu Asn Phe Phe Pro Ser Tyr Glu Lys Arg
785 790 795 800
Glu Glu Lys Leu Glu Glu Asp Ile Val Leu Cys Ala Asp Ala Ile Val
805 810 815
Asn Phe Tyr Leu Gln Thr Thr Ile Ser Asn Gly Gly Val Gln Thr Ser
820 825 830
Ile Val Ser Thr Lys Ala Leu Leu Tyr Glu Met Tyr Leu Ser Ser Ile
835 840 845
Cys Gly Gly Tyr Ser Glu Gly Val Leu Trp Tyr Leu Pro Ile Thr His
850 855 860
Pro Val Lys Cys Leu Val Ala Leu Glu Val Ser Asp Ala Leu Val Gly
865 870 875 880
Ala Asp Val Arg Ile Asp Lys Ile Arg Arg Arg Phe Pro Leu Ser Ala
885 890 895
Lys His Leu Lys Gly Ile Val Gln Ile Ser Val His Pro Asn Arg Thr
900 905 910
Phe Ser Val Thr Thr Cys Gly Ile Val Lys His Lys Val Cys Lys Lys
915 920 925
Thr Leu Leu Lys His Arg Cys Asp Val Ile Leu Leu Gln Thr Pro Gly
930 935 940
Tyr Val Phe Gly Asn Asp Glu Leu Leu Thr Lys Leu Leu Asn Ile
945 950 955
<210> 140
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV3 CAE53013.1的VP5
<400> 140
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ala
65 70 75 80
Gly Asp Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Met Lys Ile Lys Glu Ile Glu Glu Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys His Gly Lys Glu Ile Ala Lys Lys Phe Gly Asp Glu Leu Glu
115 120 125
Glu Ile Tyr Gln Phe Met Asn Gly Glu Val Lys Glu Glu Val Gln Gln
130 135 140
Glu Glu Gln Tyr Lys Val Leu Cys Lys Ala Val Asn Ser Tyr Glu Lys
145 150 155 160
Leu Leu Leu Ala Glu Asn Asp Glu Met Arg Thr Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Thr Glu Arg Thr Glu Thr Glu Ser Lys Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Ala Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Val Gly Ser
225 230 235 240
Gly Met Ala Thr Ala Ile Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Thr Met Val Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Arg Asp Ile Pro Asp Lys Glu Leu Ala Val Ser Ile Leu
290 295 300
Ala Lys Asn Asn Ala Ile Val Ala Asn Thr Arg Glu Val Gln His Ile
305 310 315 320
Lys Glu Glu Ile Leu Pro Lys Phe Lys Lys Ile Met Asp Glu Glu Lys
325 330 335
Glu Leu Glu Gly Ile Asp Asp Lys Lys Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Val Pro Arg Pro Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
Phe His Val Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Ala Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Glu Ala Lys Gly Arg Thr Leu Asn Glu Ala Tyr Lys Glu Phe
420 425 430
Leu Asn Leu Ser Ile Gly Ser Ala Tyr Thr Ser Pro Met His Ala Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Thr Leu Lys Ser Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Val
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Lys Asn Ala
515 520 525
<210> 141
<211> 956
<212> PRT
<213> 人工序列
<220>
<223> BTV4 ABB71699.1的VP2
<400> 141
Met Glu Glu Phe Val Ile Pro Val Phe Ser Glu Arg Glu Ile Pro Tyr
1 5 10 15
Ala Leu Ile Asn Arg Tyr Pro Leu Ala Ile Gln Thr Asp Val Lys Ile
20 25 30
Ala Asp Val Glu Gly Tyr His Asn Leu Ala Lys Ile Pro Glu Ser Asp
35 40 45
Met Val Asp Val Pro Lys Leu Asp Ile Val Ser Ala Leu Asn Tyr Lys
50 55 60
Pro Thr Arg Asn Asp Gly Ile Val Val Pro Arg Leu Leu Asp Ile Thr
65 70 75 80
Leu Lys Ala Tyr Asp Asp Arg Lys Ser Val Lys Asn Ala Arg Gly Val
85 90 95
Asp Phe Met Thr Asp Ala Lys Trp Met Lys Trp Ala Ile Asp Asp Arg
100 105 110
Met Asp Ile Gln Pro Leu Lys Ile Thr Leu Asp Glu His Tyr Ala Val
115 120 125
Asn His Gln Leu Phe Asn Cys Ile Val Lys Ala Lys Thr Ala Asn Ala
130 135 140
Asp Thr Ile Tyr Tyr Asp Tyr Phe Pro Leu Glu Asp Arg Ala Lys Lys
145 150 155 160
Cys Asn His Thr Asn Leu Glu Leu Leu Arg Ser Leu Thr Thr Ile Glu
165 170 175
Ala Phe His Val Leu Gln Gly Ala Ala Tyr Ser Leu Lys Ser Asn Tyr
180 185 190
Asp Leu Ile Ala Asn Ser Glu Arg Glu Ser Leu Glu Glu Ser Tyr Pro
195 200 205
Ile Gly Ser Glu Lys Trp Val His Leu Ile Arg Arg Thr Lys Ile Gly
210 215 220
Asn Ser Gly Leu Ser Tyr Asn Arg Phe Ile Ser Ser Met Val Gln Val
225 230 235 240
Val Val Arg Gly Lys Val Pro Asp Ile Ile Arg Gly Glu Ile Thr Gln
245 250 255
Leu Asn Arg Ile Arg Thr Glu Trp Ile Ser Ala Ser Tyr Asp Arg Thr
260 265 270
Arg Ile Arg Ala Leu Glu Leu Cys Asn Ile Leu Ser Ala Ile Gly Arg
275 280 285
Lys Met Met Asp Ala His Glu Glu Pro Lys Asp Glu Met Asp Leu Ser
290 295 300
Thr Arg Phe Gln Phe Lys Leu Asp Glu Lys Phe Asn Thr Ser Asp Ser
305 310 315 320
Glu His Val Asn Ile Phe Arg Thr Ser Gly Ala Ala Thr Asn Glu Gly
325 330 335
Arg Phe Tyr Ala Leu Ile Ala Ile Ala Ala Thr Asp Thr Gln Lys Gly
340 345 350
Arg Val Trp Arg Thr Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu Ile
355 360 365
Ala Ser Glu Cys Glu Leu Gly Asp Val Tyr Tyr Thr Leu Arg His Val
370 375 380
Tyr Arg Trp Ser Leu Arg Pro Glu Tyr Gly Gln Arg Glu Arg Gln Leu
385 390 395 400
Glu Asp Asn Lys Tyr Val Phe Gly Arg Val Asn Leu Phe Asp Ser Asp
405 410 415
Leu Ala Val Gly Asp Gln Val Ile His Trp Arg Tyr Glu Ile Thr Glu
420 425 430
Pro Val Lys Thr Thr Tyr Asp Asn Gly Tyr Ile Cys Asn Pro Glu Glu
435 440 445
Lys Asp Asp Glu Leu Leu Cys Lys Ile Asp Asp Glu Arg Tyr Lys Glu
450 455 460
Met Met Glu Arg Leu Ile Glu Gly Gly Trp Asp Gln Glu Arg Phe Lys
465 470 475 480
Leu His Asn Ile Leu Thr Glu Pro Asn Leu Leu Thr Ile Asp Phe Glu
485 490 495
Lys Asp Ala Tyr Leu Asn Ser Arg Ser Glu Leu Val Phe Pro Asn Tyr
500 505 510
Phe Asp Lys Trp Ile Asn Ser Pro Met Phe Asn Ala Arg Leu Arg Ile
515 520 525
Thr His Gly Glu Ile Gly Ser Ser Lys Thr Ile Asp Pro Trp Asn Arg
530 535 540
Arg Val Val Tyr Gly Tyr Ile Lys Thr Ser Ile Glu Ser Leu Asp Tyr
545 550 555 560
Thr Leu Gly Lys Tyr Tyr Asp Ile Arg Leu Gln Leu Phe Gly Asp Thr
565 570 575
Leu Ser Gln Lys Gln Thr Gln Ser Ala Val Phe Thr Tyr Leu Ser Gly
580 585 590
Gln Asp Asp Phe Pro Ala Leu Thr Asn Tyr Leu Lys Gly Glu Ala Val
595 600 605
Cys Pro His Ala Gly Gly Ala Val Tyr Thr Phe Arg Lys Met Ala Leu
610 615 620
Ser Leu Ile Ala Ser Tyr Glu Lys Leu Ser Pro Glu Met His Glu Gly
625 630 635 640
Leu Glu His Gln Thr Tyr Val His Pro Ser Val Asn Thr Ala Tyr Gln
645 650 655
Lys Gln Val Lys Asp Met Lys Asp Leu Ser Gln Leu Ile Cys Phe Ile
660 665 670
Ile Asp Cys Ile Phe Glu Lys Arg Val Gln Ile Arg Gly Val Gly Glu
675 680 685
Ala Arg Arg Ile Val Tyr Leu Ile Gln Asn Ser Thr Gly Leu Gln Arg
690 695 700
Gln Glu Val Leu Lys Arg Thr Phe Pro Asn Phe Leu Met Arg Leu Phe
705 710 715 720
Lys Leu Arg Glu Val Lys Arg Ile Cys Asp Leu Asn Val Ile Asn Phe
725 730 735
Leu Pro Leu Leu Phe Leu Val Gln Asp Asn Ile Ser Tyr Trp His Arg
740 745 750
Gln Trp Ser Val Pro Met Ile Leu Phe Asp Asp Ala Val Arg Leu Ile
755 760 765
Pro Val Glu Val Gly Ala Tyr Ala Asn Arg Phe Gly Phe Lys Ser Phe
770 775 780
Tyr Asn Phe Ile Arg Phe His Pro Gly Asp Ser Lys Lys Lys Gln Asp
785 790 795 800
Ala Asp Asp Met His Lys Glu Tyr Gly Val Ala Cys Phe Glu Tyr Tyr
805 810 815
Met Asn Thr Lys Ile Ser Gln Gly Gly Val Asn Val Pro Val Val Thr
820 825 830
Ser Lys Leu Asp Thr Leu Lys Ile His Leu Ala Ser Leu Cys Ser Gly
835 840 845
Leu Ala Asp Ser Ile Val Tyr Thr Leu Pro Val Ala His Pro Lys Lys
850 855 860
Cys Ile Val Leu Ile Val Val Gly Asp Asp Lys Leu Asp Pro Gln Val
865 870 875 880
Arg Ser Glu Gln Val Leu Ser Lys Tyr Tyr Tyr Ser Arg Arg His Ile
885 890 895
Cys Gly Val Val Ala Val Ser Val Gly Gln Glu Gly Gln Leu Gln Val
900 905 910
Tyr Ser Thr Gly Ile Val Arg His Arg Ile Cys Glu Lys Ser Ile Leu
915 920 925
Lys Tyr Lys Cys Lys Val Val Leu Val Arg Met Pro Gly His Val Phe
930 935 940
Gly Asn Asp Glu Leu Met Thr Lys Leu Leu Asn Val
945 950 955
<210> 142
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV 4 CAE53015.1的VP5
<400> 142
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Ser Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ala Ala Thr Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Thr
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Met Gln
85 90 95
Thr Lys Ile Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Lys Glu Ile Thr Lys Glu Phe Gly Lys Glu Leu Glu
115 120 125
Glu Val Tyr Asp Phe Met Asn Gly Glu Ala Lys Glu Glu Glu Val Val
130 135 140
Gln Glu Gln Tyr Ser Met Leu Cys Lys Ala Val Asp Ser Tyr Glu Lys
145 150 155 160
Ile Leu Lys Ala Glu Asp Ser Lys Met Ala Ile Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Ser Glu Arg Ser Gln Asp Glu Ile Lys Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Asp Ala Ile Glu Ile Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Met Arg Ser Pro Lys Ile Glu Pro Thr Ile Ile Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Lys Glu Ile Pro Asp Glu Gln Leu Ala Val Ser Val Leu
290 295 300
Asn Lys Lys Thr Ala Val Thr Asp Asn Cys Asn Glu Ile Ala His Ile
305 310 315 320
Lys Gln Glu Ile Leu Pro Lys Phe Lys Gln Ile Met Asp Glu Glu Lys
325 330 335
Glu Ile Glu Gly Ile Glu Asp Lys Val Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Ile Pro Arg Thr Gln Gln Pro Gln Ile His Ile Tyr Ala
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Arg Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Thr Ser His Trp His Ala Leu Gly Leu
405 410 415
Ala Gln Glu Ala Ser Gly Arg Thr Leu Thr Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ser Ile Ser Asn Thr Tyr Ser Ser Ala Ile His Ala Arg
435 440 445
Arg Met Ile Arg Ser Arg Ala Val His Pro Ile Phe Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Ala Leu Lys Asn Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Glu Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Ile
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 143
<211> 956
<212> PRT
<213> 人工序列
<220>
<223> BTV4 CAE51091.1的VP2
<400> 143
Met Glu Glu Phe Val Ile Pro Val Phe Ser Glu Arg Glu Ile Pro Tyr
1 5 10 15
Ala Leu Ile Asn Arg Tyr Pro Leu Ala Ile Gln Thr Asp Val Lys Ile
20 25 30
Val Asp Val Asp Gly Tyr His Asn Leu Ala Lys Ile Pro Glu Ser Asp
35 40 45
Met Val Asp Val Pro Lys Leu Asp Ile Val Ser Ala Leu Asn Tyr Lys
50 55 60
Pro Thr Arg Asn Asp Gly Ile Val Val Pro Arg Leu Leu Asp Ile Thr
65 70 75 80
Leu Lys Ala Tyr Asp Asp Arg Lys Ser Val Lys Asn Ala Arg Gly Val
85 90 95
Asp Phe Met Thr Asp Ala Lys Trp Met Lys Trp Ala Ile Asp Asp Arg
100 105 110
Met Asp Ile Gln Pro Leu Lys Ile Thr Leu Asp Glu His Tyr Ala Val
115 120 125
Asn His Gln Leu Phe Asn Cys Ile Val Lys Ala Lys Thr Ala Asn Ala
130 135 140
Asp Thr Ile Tyr Tyr Asp Tyr Phe Pro Leu Glu Asp Arg Ala Lys Lys
145 150 155 160
Cys Asn His Thr Asn Leu Glu Leu Leu Arg Ser Leu Thr Thr Ile Glu
165 170 175
Ala Phe His Val Leu Gln Gly Ala Ala Tyr Ser Leu Lys Ser Asn Tyr
180 185 190
Asp Leu Ile Ala Asn Ser Glu Arg Glu Ser Leu Glu Glu Ser Tyr Pro
195 200 205
Ile Gly Ser Glu Lys Trp Val His Leu Thr Arg Arg Ala Lys Ile Gly
210 215 220
Asn Ser Gly Leu Ser Tyr Asn Arg Phe Ile Ser Ser Met Val Gln Val
225 230 235 240
Val Val Arg Gly Lys Val Pro Asp Ile Ile Arg Gly Glu Ile Thr Gln
245 250 255
Leu Asn Arg Ile Arg Thr Glu Trp Ile Asn Ala Ser Tyr Asp Arg Thr
260 265 270
Arg Ile Arg Ala Leu Glu Leu Cys Asn Ile Leu Ser Ala Ile Gly Arg
275 280 285
Lys Met Met Asp Ala His Glu Glu Pro Lys Asp Glu Met Asp Leu Ser
290 295 300
Thr Arg Phe Gln Phe Lys Leu Asp Glu Lys Phe Asn Thr Ser Asp Ser
305 310 315 320
Glu His Val Asn Ile Phe Arg Thr Ser Gly Ala Ala Thr Asn Glu Gly
325 330 335
Arg Phe Tyr Ala Leu Ile Ala Ile Ala Ala Thr Asp Thr Gln Lys Gly
340 345 350
Arg Val Trp Arg Thr Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu Ile
355 360 365
Ala Ser Glu Cys Glu Leu Gly Asp Val Tyr Tyr Thr Leu Arg His Val
370 375 380
Tyr Arg Trp Ser Leu Arg Pro Glu Tyr Gly Gln Arg Glu Arg Gln Leu
385 390 395 400
Glu Asp Asn Lys Tyr Val Phe Gly Arg Val Asn Leu Phe Asp Ser Asp
405 410 415
Leu Ala Val Gly Asp Gln Val Ile His Trp Arg Tyr Glu Ile Thr Glu
420 425 430
Pro Val Lys Thr Thr Tyr Asp Asn Gly Tyr Ile Cys Asn Pro Glu Glu
435 440 445
Lys Asp Asp Glu Leu Leu Cys Lys Ile Asp Asp Glu Arg Tyr Lys Glu
450 455 460
Met Met Glu Arg Leu Ile Glu Gly Gly Trp Asp Gln Glu Arg Phe Lys
465 470 475 480
Leu His Asn Ile Leu Thr Glu Pro Asn Leu Leu Thr Ile Asp Phe Glu
485 490 495
Lys Asp Ala Tyr Leu Asn Ser Arg Ser Glu Leu Val Phe Pro Asn Tyr
500 505 510
Phe Asp Lys Trp Ile Asn Ser Pro Met Phe Asn Ala Arg Leu Arg Ile
515 520 525
Thr His Gly Glu Ile Gly Ser Ser Lys Thr Ala Asp Pro Trp Asn Arg
530 535 540
Arg Val Val Tyr Gly Tyr Ile Lys Thr Ser Ile Glu Ser Leu Asp Tyr
545 550 555 560
Thr Leu Gly Lys Tyr Tyr Asp Ile Arg Leu Gln Leu Phe Gly Asp Thr
565 570 575
Leu Ser Arg Lys Gln Thr Gln Ser Ala Val Phe Thr Tyr Leu Ser Gly
580 585 590
Gln Asp Asp Phe Pro Thr Leu Thr Asn Tyr Leu Lys Ser Glu Ala Val
595 600 605
Cys Pro His Ala Gly Gly Ala Val Tyr Thr Phe Arg Lys Met Ala Leu
610 615 620
Ser Leu Ile Ala Asn Tyr Glu Lys Leu Asn Pro Glu Met His Glu Gly
625 630 635 640
Leu Glu His Gln Thr Tyr Val His Pro Ser Val Asn Thr Thr Tyr Gln
645 650 655
Lys Gln Val Lys Asp Met Lys Asp Phe Ser Gln Leu Ile Cys Phe Ile
660 665 670
Ile Asp Cys Ile Phe Glu Lys Arg Ile Gln Ile Arg Gly Val Gly Glu
675 680 685
Ala Arg Arg Ile Ile Tyr Leu Ile Gln Asn Ser Ile Gly Leu Gln Arg
690 695 700
Gln Glu Val Leu Lys Arg Thr Phe Pro Asn Phe Leu Met Arg Leu Phe
705 710 715 720
Lys Leu Arg Glu Val Lys Arg Ile Cys Asp Leu Ser Val Ile Asn Phe
725 730 735
Leu Pro Leu Leu Phe Leu Val Gln Asp Asn Ile Ser Tyr Trp His Arg
740 745 750
Gln Trp Ser Val Pro Met Ile Leu Phe Asp Asp Ala Val Arg Leu Ile
755 760 765
Pro Val Glu Val Gly Ala Tyr Ala Asn Arg Phe Gly Phe Lys Ser Phe
770 775 780
Tyr Asn Phe Ile Arg Phe His Pro Gly Asp Ser Lys Lys Lys Gln Asp
785 790 795 800
Ala Asp Asp Met His Lys Glu Tyr Gly Val Ala Cys Phe Glu Tyr Tyr
805 810 815
Met Asn Thr Lys Ile Ser Gln Gly Gly Val Asn Val Pro Val Val Thr
820 825 830
Ser Lys Leu Asp Thr Leu Lys Ile His Leu Ala Ser Leu Cys Leu Gly
835 840 845
Leu Ala Asp Ser Ile Val Tyr Thr Leu Pro Val Ala His Pro Lys Lys
850 855 860
Cys Ile Val Leu Ile Val Val Gly Asp Asp Lys Leu Asp Pro Gln Val
865 870 875 880
Arg Ser Glu Gln Val Leu Ser Lys Tyr Tyr Tyr Ser Arg Arg His Ile
885 890 895
Cys Gly Val Val Ala Val Ser Val Gly Gln Glu Gly Gln Leu Gln Val
900 905 910
Tyr Ser Ser Gly Ile Val Arg His Arg Ile Cys Glu Lys Ser Ile Leu
915 920 925
Lys Tyr Lys Cys Lys Val Val Leu Val Arg Met Pro Gly His Val Phe
930 935 940
Gly Asn Asp Glu Leu Met Thr Lys Leu Leu Asn Val
945 950 955
<210> 144
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV4 CAE53015.1的VP5
<400> 144
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Ser Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ala Ala Thr Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Thr
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Met Gln
85 90 95
Thr Lys Ile Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Lys Glu Ile Thr Lys Glu Phe Gly Lys Glu Leu Glu
115 120 125
Glu Val Tyr Asp Phe Met Asn Gly Glu Ala Lys Glu Glu Glu Val Val
130 135 140
Gln Glu Gln Tyr Ser Met Leu Cys Lys Ala Val Asp Ser Tyr Glu Lys
145 150 155 160
Ile Leu Lys Ala Glu Asp Ser Lys Met Ala Ile Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Ser Glu Arg Ser Gln Asp Glu Ile Lys Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Asp Ala Ile Glu Ile Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Met Arg Ser Pro Lys Ile Glu Pro Thr Ile Ile Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Lys Glu Ile Pro Asp Glu Gln Leu Ala Val Ser Val Leu
290 295 300
Asn Lys Lys Thr Ala Val Thr Asp Asn Cys Asn Glu Ile Ala His Ile
305 310 315 320
Lys Gln Glu Ile Leu Pro Lys Phe Lys Gln Ile Met Asp Glu Glu Lys
325 330 335
Glu Ile Glu Gly Ile Glu Asp Lys Val Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Ile Pro Arg Thr Gln Gln Pro Gln Ile His Ile Tyr Ala
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Arg Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Thr Ser His Trp His Ala Leu Gly Leu
405 410 415
Ala Gln Glu Ala Ser Gly Arg Thr Leu Thr Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ser Ile Ser Asn Thr Tyr Ser Ser Ala Ile His Ala Arg
435 440 445
Arg Met Ile Arg Ser Arg Ala Val His Pro Ile Phe Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Ala Leu Lys Asn Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Glu Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Ile
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 145
<211> 955
<212> PRT
<213> 人工序列
<220>
<223> BTV 5 CAE51092.1的VP2
<400> 145
Met Asp Glu Phe Gly Val Pro Ile Phe Arg Ser Ser Gln Val Pro His
1 5 10 15
His Ile Leu Glu Gly Tyr Asp Ile Lys Val Asp Leu Ser Asp Arg Val
20 25 30
Val Gln Glu Asp Gly Arg His Asp Pro Asn Lys Leu Lys Glu Arg Lys
35 40 45
Thr Ile Asp Val Pro Asn Gly Glu Ala Arg Ser Asp Leu Asn Tyr Asn
50 55 60
Pro Thr Asp Asn Glu Gly Asn Gln Leu Pro Arg Ala Leu Asp Ile Ala
65 70 75 80
Leu Cys Ala Arg Leu Thr Arg Lys Ser Phe Asn Thr Asn Ser Gly Val
85 90 95
Thr Phe His Thr Asp His Lys Trp Met Glu Trp Met Leu Ser Asp Ala
100 105 110
Met Asp Val Gln Pro Leu Arg Ile Asp Leu Ala Ala Gln Asn Glu Tyr
115 120 125
Ala Lys Cys Asp Leu Phe Asn Ser Ser Val Tyr Val Arg Lys Lys Tyr
130 135 140
Val Asp Ala Ile Ser Tyr Arg Tyr Ile Ser Val Glu Asp Asp Ser Lys
145 150 155 160
Gly Cys Asn His Thr Arg Val Tyr Ser Val Asn His Met Ile Asn Cys
165 170 175
Gly Leu Tyr Asn Val Ala Gln Glu Cys Ala Tyr Ala Phe Lys His Thr
180 185 190
Asn Thr Leu Ile Val Asn Ser Gln Arg Glu Thr Thr Ser Glu Pro Phe
195 200 205
Gln Pro Gly Asn Pro Lys Ile Gly Ser Leu Gly Arg Arg Ala Arg Val
210 215 220
Asp Met Ala Asp Pro Gly Tyr Pro Leu Phe Arg Ala Gly Leu Leu Gln
225 230 235 240
Ile Ile Val Asn Gly Ala Ile Pro Ala Asp Ile Arg Ser Glu Met Asp
245 250 255
Arg Leu Asn Gln Ile Arg Glu Ala Trp Lys Arg Asp Lys Asn Thr Arg
260 265 270
Glu Val Arg Ala Leu Asp Leu Cys Val Leu Leu Ser Lys Ile Gly Arg
275 280 285
Ile Lys Met Asn Met Glu Glu Glu Pro Lys Asp Glu Gly Val Met Ser
290 295 300
Leu Arg Phe Gln Gly Lys Ile Asp Ala Met Phe Phe Ser Glu Asn Thr
305 310 315 320
Glu Lys Thr Asn Ile Met Arg Asp Gly Ser Gly Arg Thr Asp Asp Glu
325 330 335
Arg Phe Tyr Ala Leu Leu Leu Ile Cys Ala Thr Asp Ala Phe Ser Arg
340 345 350
Arg Ile Trp Arg Thr Asn Pro Tyr Pro Cys Leu Arg Gly Thr Leu Ile
355 360 365
Ala Ala Glu Cys Val Leu Gly Asp Pro Tyr Lys Thr Leu Arg Arg Lys
370 375 380
Phe Asp Trp Ser Val Arg His Ala Gly Asp Lys Met Leu Glu Gly Ser
385 390 395 400
Ser Tyr Val Phe Thr Arg Ile Asn Leu Phe Asp Thr Asp Lys Ala Pro
405 410 415
Gly Ser Arg Val Ile His Trp Ile Gln Glu Thr Leu Pro Val Ile Lys
420 425 430
Thr Thr Trp Ala Glu Gly Cys Ser Leu Ala Asp Glu Ala Ser Asp Asp
435 440 445
His Leu His Cys Lys Ile Asp His Ala Gln Tyr Arg Asp Leu Val Ser
450 455 460
Arg Val Ile Asn Gly Gly Trp Asp Gln Glu Asn Phe Lys Ile His Lys
465 470 475 480
Leu Phe Val Glu Arg Gly Asn Ile Phe Leu Met Asp Phe Glu Lys Asp
485 490 495
Ala Lys Ile Thr Thr Gln Ser Glu Val Glu Tyr Pro Tyr Tyr Tyr Asp
500 505 510
Thr Trp Ile Tyr Ala Pro Ile Phe Ser Thr Arg Tyr Lys Ile Thr Glu
515 520 525
Thr Glu Ile Ala Asn Ala Lys Ser Leu Asp Pro Ala Ile Lys Arg Thr
530 535 540
Leu Ala Pro Leu Ser Asp Asp Pro Val Ala Leu Gln Leu Arg Ala Val
545 550 555 560
Gly His Leu Tyr Asp Thr Arg Pro Ala Ile Met Gly Gln Thr Leu Ser
565 570 575
Pro Arg Gln Asn Gln Thr Ala Leu His Lys Thr Leu Leu Asp Asp Pro
580 585 590
Glu Asn Asp Arg Tyr Tyr Glu Ala Arg Ser Ile Lys Lys Val Asp Asn
595 600 605
Pro Cys Ser Val His Tyr Thr Ser Ser Phe Ile Leu Gln Lys Ile Ser
610 615 620
Glu Leu Val Leu Ser Leu Met Ile Tyr His Ile Glu His Asp Trp Glu
625 630 635 640
Pro Leu Lys Gln Tyr Thr His Pro Glu Ile Ser Thr Glu Phe Gly Glu
645 650 655
Ile Ser Cys Glu Ile Cys Asp Ile Ser Gln Leu Thr Val Leu Leu Ile
660 665 670
Asp Leu Phe Phe Glu Asn Arg Arg Thr Val Arg Pro Ser Glu Glu Thr
675 680 685
Arg Tyr Ile Ile His Gln Ile Arg Glu Ser Arg Gly Asp Gly Arg Leu
690 695 700
Lys Met Leu Glu Arg Phe Phe Pro Ala Tyr Gly Gln Ala Leu Arg Lys
705 710 715 720
Ile Glu Ser Ala Thr Glu Val Gly His Ile Thr Met Leu Asn Phe Leu
725 730 735
Pro Phe Phe Phe Leu Leu Gly Asp Asn Ile Ile Tyr Lys His Arg Glu
740 745 750
Trp Thr Val Pro Ile Leu Ile Tyr Thr Asp Gly Val Met Ile Trp Pro
755 760 765
Ala Gln Val Gly Ala Asn Tyr Asn Arg Phe Gly Phe Cys Gly Phe Leu
770 775 780
Asn Tyr Met Arg Phe His Ala Gly Ser Gln Leu Arg Ser Lys Val Leu
785 790 795 800
Asp Asp Ala Glu Lys Thr Ile Ala Lys Glu Met Leu Lys Tyr Tyr Met
805 810 815
Lys Thr Asp Ile Phe Ser Gly Gly Val Gln Lys Asn Ile Arg Val Thr
820 825 830
Lys Ser Met Gln Met Glu Ile Tyr His Ser Ser Leu Cys Gly Gly Leu
835 840 845
Ser Asp Ala Val Val Tyr Ala Leu Pro Ile Ser Phe Pro Ile Lys Cys
850 855 860
Leu Thr Leu Ile Ile Ile Gly Asp Asp Leu Val Glu Pro Gln Leu Arg
865 870 875 880
Ile Ser Arg Val Met Asp Tyr Phe Lys His Val Ser Glu His Val Arg
885 890 895
Gly Val Ala Ser Ile Ser Ile Ser Arg Asn Gly Asp Val Ser Thr Tyr
900 905 910
Ser Arg Gly Ile Val His Val Glu Leu Leu Lys Lys Asn Ile Leu Lys
915 920 925
Tyr Gln Phe Gln Val Ala Leu Leu Lys Val Lys Gly Tyr Val Phe Gly
930 935 940
Asn Asp Glu Met Leu Ile Lys Leu Leu Asn Val
945 950 955
<210> 146
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV5 CAE53016.1的VP5
<400> 146
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ala
65 70 75 80
Gly Asp Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Met Lys Ile Lys Glu Ile Glu Glu Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys His Gly Lys Glu Ile Thr Arg Lys Phe Gly Ala Glu Leu Glu
115 120 125
Glu Ile Tyr Gln Phe Met Asn Gly Glu Met Arg Glu Glu Ala Glu Gln
130 135 140
Glu Glu Gln Tyr Lys Val Leu Cys Lys Ala Val Asn Ser Tyr Glu Lys
145 150 155 160
Leu Leu Val Ala Glu Asn Asp Gln Met Arg Ala Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Thr Glu Arg Thr Glu Ala Glu Ser Thr Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Ala Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Val Gly Ser
225 230 235 240
Gly Met Ala Thr Ala Ile Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Thr Met Val Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Asp Glu Ile Pro Asp Lys Glu Leu Ala Val Ser Ile Leu
290 295 300
Ala Lys Asn Asn Ala Ile Val Ala Asn Thr Arg Glu Val Gln His Ile
305 310 315 320
Lys Asp Glu Ile Leu Pro Lys Phe Lys Lys Ile Met Asp Glu Glu Lys
325 330 335
Glu Leu Glu Gly Ile Asp Asp Lys Lys Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Val Pro Arg Ser Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
Phe His Ala Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Ala Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Glu Ala Lys Gly Arg Thr Leu Ser Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ser Ile Gly Ser Ala Tyr Thr Ser Pro Met His Ala Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Thr Leu Lys Ser Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Val
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 147
<211> 955
<212> PRT
<213> 人工序列
<220>
<223> BTV6 CAE51093.1的VP2
<400> 147
Met Asp Glu Leu Ala Ile Pro Ile Ile Asn Gly His Phe Pro Lys Glu
1 5 10 15
Ala Leu Ile Glu Tyr Asp Cys Ile Val Glu Leu Asn Ala Pro Ser Glu
20 25 30
Asp Asp Glu Val Asp Val Thr Gln Ile Pro Thr Lys Asn Met Met Asp
35 40 45
Ile Pro Asp Val Pro Ile Arg Glu Ala Leu Arg Val Leu Pro Gln Arg
50 55 60
Asn Asp Gly His Val Leu Thr Arg Ile Leu Asp Ile Ser Leu Ser Gly
65 70 75 80
Tyr Asp Arg Arg Lys Tyr Tyr Lys Thr Asn Lys Gly Gly Glu Phe Glu
85 90 95
Ser Pro Lys Glu Trp Leu Asn Trp Met Ile His Asp Ser Met Asp Val
100 105 110
Gln Pro Leu Lys Val Ser Leu Glu Met Arg Pro Thr Val Lys His Glu
115 120 125
Leu Phe Ser Ser Asn Val Tyr Leu Ser Val Lys Lys Ala Asp Thr Ser
130 135 140
Ser Tyr His Ile Glu Ala Ile Gly Asp Thr Gly Lys Ala Cys Asp His
145 150 155 160
Ser Tyr Val Ser Met Trp Asn Asn Leu Val Arg His Glu Val Met His
165 170 175
Cys Ala Gln Glu Ser Ala Tyr Val Leu Lys Pro Thr Met Glu Ile Ile
180 185 190
Val His Ser Glu Arg Ala Ser Thr Asp Val Pro Phe Gln Leu Val Gly
195 200 205
Gln Asn Tyr Val Ser Ile Gly Arg Asn His Arg Leu Val Leu Gly Asp
210 215 220
Glu Pro Tyr Lys Lys Thr Leu Gln Gly Leu Thr Lys Leu Arg Val Gln
225 230 235 240
Gly Lys Val Pro Pro Gln Ile Gln Asp Glu Ile Gln Gln Leu Asn Arg
245 250 255
Ile Arg Asp Ala Trp Ile Gly Asp Ala Tyr Asn Pro Arg His Ile Arg
260 265 270
Ser Leu Glu Leu Cys Arg Ile Leu Ser Ser Val Gly Arg Lys Val Val
275 280 285
Cys Ile Glu Glu Glu Pro Lys Asn Glu Ser Asp Ile Ser Val Lys Phe
290 295 300
Gln Phe Lys Leu Asp Glu Lys Phe Arg Leu Asn Asp Gly Glu Arg Gly
305 310 315 320
Val Ile Phe Thr Ser Lys Asn Gln Arg Asn Asp Gln Asp Arg Phe Phe
325 330 335
Val Leu Ile Met Ile Ala Ala Ser Asp Thr Tyr Asn Gly Arg Ile Trp
340 345 350
Trp Thr Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu Ile Ala Ser Glu
355 360 365
Thr Arg Leu Gly Asp Val Tyr Trp Thr Leu Arg His Thr Tyr Asp Trp
370 375 380
Ser Val Arg Met Thr Tyr Ser Pro Arg Met Lys Glu Arg Glu Asp Asp
385 390 395 400
Lys Tyr Ile Tyr Gly Arg Val Asn Leu Phe Asp Leu Asp Ala Ser Pro
405 410 415
Gly Asp Lys Val Val His Trp Arg Tyr Glu Leu Ile Ser Gln Thr Lys
420 425 430
Lys Thr Thr Phe His Asp Gly Asn Pro Cys Asp Leu Phe Pro Asp Val
435 440 445
Glu Gly Ile Val Thr Lys Phe Asn Lys Thr Ala Tyr Ser Asn Met Ile
450 455 460
Asn Glu Leu Leu Asn Gly Gly Trp Asn Thr Arg Glu Phe Lys Met Tyr
465 470 475 480
Lys Leu Leu Glu Asp Glu Gly Asn Val Leu Thr Ile Asp Leu Glu Lys
485 490 495
Asp Thr Lys Leu Asn Ser Arg Ser Glu Phe Ile Leu Pro Asn Tyr Tyr
500 505 510
Asp Lys Trp Ile Tyr Ala Pro Met Phe Asn Ala Lys Leu Lys Ile Thr
515 520 525
Glu Thr Glu Ile Ala Gln Ala Lys Asn Asp Asp Pro Tyr Val Ile Arg
530 535 540
Thr Leu Ala Pro Ile Gly Ser Asn Pro Phe Thr Leu Gln Arg Leu Cys
545 550 555 560
Leu Gly Asn Tyr Tyr Asp Val Arg Pro Ala Met His Gly Arg Ala Leu
565 570 575
Ser Lys Lys Gln Glu Gln Ser Ala Tyr His Ala Ala Leu Val Lys Glu
580 585 590
Lys Asp Tyr Glu Asp Ile Leu Thr Arg Arg Gly Leu Arg Lys Ala Asp
595 600 605
Lys Pro Cys Pro Leu Thr Thr Cys Ala Tyr Ile Leu Glu Lys Thr Ala
610 615 620
Leu Leu Trp Ile Asp Val Met Glu Lys His Ile Ser Glu Val Thr Asp
625 630 635 640
Asp Ser Asn Glu Phe Asp Phe Pro Lys Ala Cys Val Asp Ser Pro Val
645 650 655
Gly Thr Asp Arg Ile Val Asp Val Thr Gln Leu Ile Val Leu Leu Ile
660 665 670
Asp Trp Ile Tyr Glu Arg Arg Lys Asp Val Arg Ser Val Asp Glu Ser
675 680 685
Arg Trp Val Ile Glu Thr Ile Arg Arg Ala Arg Gly Arg Asn Arg Leu
690 695 700
Glu Leu Ile Ser Arg Glu Phe Pro Asn Phe Gly Arg Ala Val Ala Arg
705 710 715 720
Leu Gln Ser Pro Asn Thr Val Gly Asp Val Met Val Ile Asn Phe Leu
725 730 735
Pro Phe Leu Phe Leu Met Gly Asp Asn Val Ser Tyr Glu His Arg Gln
740 745 750
Trp Ser Val Pro Val Ile Leu His Ala Asp Val Met Trp Phe Ile Pro
755 760 765
Val Glu Val Gly Ala Phe Tyr Asn Arg Phe Gly Val Thr Ser Phe Leu
770 775 780
Glu Tyr Leu Met Phe Phe Pro Ser Phe His Met Arg Thr Val Arg Leu
785 790 795 800
Asp Glu Ala Glu Val Lys Val Ser Lys Leu Ile Leu Asp Phe Tyr Leu
805 810 815
Asp Thr Thr Ile Phe Ser Gly Gly Ile Gln Ser Ser Val Val Ser Thr
820 825 830
Lys Gln Ile Leu Tyr Glu Thr Tyr Leu Ser Ser Leu Cys Gly Gly Phe
835 840 845
Ser Glu Gly Leu Val Trp Tyr Leu Pro Ile Thr His Pro Thr Lys Cys
850 855 860
Val Val Ala Ile Glu Phe Ala Asp Asn Lys Val Ser Ala Val Tyr Arg
865 870 875 880
Cys Asn Arg Leu Arg Ala Arg Phe Pro Leu Ser Ser Ile His Leu Arg
885 890 895
Gly Ile Ala Ile Leu Thr Ile Gly Lys Asn Arg Met Val Asp Ala Tyr
900 905 910
Thr Glu Gly Ile Val Ser His Arg Ile Cys Lys Lys Asn Val Leu Gly
915 920 925
Tyr Thr Cys Gln Ile Leu Leu Leu Lys Phe Ser Gly His Val Phe Gly
930 935 940
Asn Asp Glu Met Leu Thr Lys Leu Leu Asn Val
945 950 955
<210> 148
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV6 CAE53019.1的VP5
<400> 148
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ala
65 70 75 80
Gly Asp Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Met Lys Ile Lys Glu Ile Glu Glu Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys His Gly Lys Glu Ile Ala Lys Lys Phe Gly Asp Glu Leu Glu
115 120 125
Glu Ile Tyr Gln Phe Met Asn Gly Glu Val Lys Glu Glu Val Gln Gln
130 135 140
Glu Glu Gln Tyr Lys Val Leu Cys Lys Ala Val Asn Ser Tyr Glu Lys
145 150 155 160
Leu Leu Leu Ala Glu Asn Asp Glu Met Arg Thr Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Thr Glu Arg Thr Glu Thr Glu Ser Lys Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Ala Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Val Gly Ser
225 230 235 240
Gly Met Ala Thr Ala Ile Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Thr Met Val Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Arg Asp Ile Pro Asp Lys Glu Leu Ala Val Ser Ile Leu
290 295 300
Ala Lys Asn Asn Ala Ile Val Ala Asn Thr Arg Glu Val Gln His Ile
305 310 315 320
Lys Glu Glu Ile Leu Pro Lys Phe Lys Lys Ile Met Asp Glu Glu Lys
325 330 335
Glu Leu Glu Gly Ile Asp Asp Lys Lys Ile His Pro Arg Val Met Met
340 345 350
Lys Phe Lys Val Pro Arg Ser Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
Phe His Val Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Ala Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Glu Ala Lys Gly Arg Thr Leu Asn Glu Ala Tyr Lys Glu Phe
420 425 430
Leu Asn Leu Ser Ile Gly Ser Ala Tyr Thr Ser Pro Met His Ala Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Thr Leu Lys Ser Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Val
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Lys Asn Ala
515 520 525
<210> 149
<211> 959
<212> PRT
<213> 人工序列
<220>
<223> BTV7 CAE51094.1的VP2
<400> 149
Met Asp Glu Phe Ala Ile Pro Ile Val His Gly Arg Val Ile Pro Tyr
1 5 10 15
Asp Leu Val Arg Ser Tyr Asp Ile Val Val Asp Leu Asp Val Arg Ile
20 25 30
Asp Glu Glu Gly Asp Lys His Asp Thr Thr Lys Ile Pro Glu Ala Tyr
35 40 45
Met Cys Asp Val Pro Asn Ile Ala Met Gln Asp Ala Asn Ser Ala Ile
50 55 60
Leu Gly Arg Asn Asp Gly Tyr Ala Leu Pro Arg Ile Leu Asp Val Ser
65 70 75 80
Ile Ala Ala Tyr Asp Pro Arg Lys Gly Tyr Trp Lys Tyr Arg Gly Ile
85 90 95
Glu Lys Met Thr Asn Gln Thr Trp Leu Lys Trp Ala Ile Lys Asp Phe
100 105 110
Met Asp Val Gln Pro Leu Lys Val Asp Leu Ala Asp Leu Glu Gly Lys
115 120 125
Val Arg Tyr Asp Met Phe Asn Cys Ser Ala Arg Ile Asp Ala Lys Lys
130 135 140
Ala Glu Ala Ile Tyr Tyr Gly Trp Ala Ala Gln Glu Val Val Asn Lys
145 150 155 160
Gly Cys Asn His Ala Lys Ser Glu Met Phe Gln His Val Val Arg Cys
165 170 175
Glu Leu Leu Asn Cys Ser Gln Glu Ser Ala Tyr Ile Leu Lys Pro Thr
180 185 190
Gln Lys Ile Glu Pro Leu Ala Glu Lys Asn Asp Leu Gly Glu Arg Leu
195 200 205
Gly Pro Gly Ser Asn Asn Leu Ile Asn Met Arg Arg Gly Asp Thr Phe
210 215 220
Arg Met Gly Thr Asp Gln Tyr Asn Arg Met Leu Gln Gly Trp Tyr Arg
225 230 235 240
Val Lys Val Lys Gly Gln Ile Pro Asp Met Val Arg Glu Glu Met Ala
245 250 255
Glu Leu Val Lys Ile Arg Asp Arg Trp Lys Val Ala Lys Asn Pro Arg
260 265 270
Glu Ile Lys Ser Leu Glu Leu Cys Lys Leu Leu Ser Thr Ile Gly Arg
275 280 285
Arg Met Tyr Asn Met Tyr Glu Glu Pro Lys Asp Glu Glu Ala Val Ser
290 295 300
Leu Arg Phe Gln Trp Asn Leu Asp Glu Ile Phe Lys Thr Asp Asn Arg
305 310 315 320
Glu His Val Asn Ile Ala Val Asn Lys Asn Gly Arg Thr Asp Asp Glu
325 330 335
Arg Phe Phe Ala Leu Ile Met Ile Ala Ala Thr Asp Thr Asn Arg Gly
340 345 350
Arg Ile Trp Trp Ser Asn Met Phe Pro Cys Leu Arg Gly Ala Leu Ile
355 360 365
Ala Ser Glu Thr Gln Phe Gly Asp Val Tyr Lys Met Leu Arg Val Arg
370 375 380
Phe Glu Trp Ser Val Arg Asn Glu Tyr Ser Pro Gln Ala Asp Val Ala
385 390 395 400
Arg Gln Asn Glu Asn Tyr Val Tyr Ala Arg Val Asn Leu Phe Asp Leu
405 410 415
Val Ala Asp Pro Ala Thr Ser Val Ile His Trp Glu Tyr Lys Leu Asp
420 425 430
Lys Pro Phe Lys Thr Cys Tyr Ser Asp Gly His Gln Cys Asp Glu Tyr
435 440 445
Pro Asp Glu Tyr Asp Leu Ile Cys Ser Phe Asp Glu Ala Lys Tyr Ser
450 455 460
Glu Met Val Gln Arg Ile Ile Glu Gly Gly Trp Asp His Lys Asn Phe
465 470 475 480
Lys His Tyr Lys Ile Leu Lys Glu Ala Ser Asn Val Leu Thr Ile Asp
485 490 495
Phe Glu Lys Asp Ala Lys Leu Asn Glu Ser Ser Glu Met Asn Leu Pro
500 505 510
Tyr Tyr Phe Asn Lys Asp Ile Ala Ala Pro Met Phe Arg Cys Val Leu
515 520 525
Lys Val Cys Glu Thr Ser Ile Ser Glu Arg His Asn Leu Asp Pro Ala
530 535 540
Ile Ser Arg Thr Met Lys Pro Met Arg Ala Lys Ala Ala Asp Leu Leu
545 550 555 560
Asp Tyr Ser Leu Gly Ser Tyr Tyr Glu Thr Arg Ser Gly Val Met Gly
565 570 575
Arg Thr Leu Thr Thr Thr Gln Lys Gln Ser Asp Met Tyr Lys Met Val
580 585 590
Gln Asp Glu Asp Asp Tyr Leu Ser Leu Met Val Gly Arg Gly Val Thr
595 600 605
Asp Lys Phe Ala Asn Pro Cys Pro Ile Thr Thr Ala Gly Gln Tyr Ala
610 615 620
Leu Gln Lys Thr Ser Val Met Ile Phe Ser Arg Val Glu His Tyr Phe
625 630 635 640
Glu Glu Tyr Leu Lys Asp Asp Lys Val Glu Tyr Leu Ser Pro Ser Leu
645 650 655
Asn Leu Glu Trp Val Glu Ile Thr Ser Arg Ile Thr Asp Ile Ser Gln
660 665 670
Leu Ile Val Leu Thr Phe Asp Val Phe Phe Glu Gly Arg Arg Gln Val
675 680 685
Arg Thr Ile Asp Glu Ser Arg Phe Ile Leu Tyr Gln Ile Arg Ala Arg
690 695 700
Lys Gly Met Asp Arg Leu Ser Tyr Ile Lys Asp Leu Phe Pro Gln Leu
705 710 715 720
Tyr Gly Trp Trp Val Ser Pro Glu Pro Thr Tyr Gly His Ala Gln Lys
725 730 735
Leu Asn Leu Leu Pro Leu Leu Phe Ile Ile Gly Asp Asn Phe Ile Tyr
740 745 750
Glu His Arg Gln Trp Ser Tyr Pro Val Ile Leu Val Ser His Lys Leu
755 760 765
Arg Phe Val Pro Val Glu Leu Gly Ala Tyr Tyr Asn Arg Phe Gly Leu
770 775 780
Thr Ser Leu Phe Ser Tyr Leu Val Phe His Val Gly Lys Ser Lys Tyr
785 790 795 800
Gly Leu Glu Leu Asp Glu Ala Glu Lys Lys Val Leu Gln Ala Ala Lys
805 810 815
Asp Tyr Tyr Ala Val Ala Thr Ile Ser Glu Gly Gly Ile Asn Thr His
820 825 830
Ser Thr Met Thr Lys Asp Pro Ile Tyr Gln Val Tyr Leu Ser Thr Ile
835 840 845
Cys Gly Gly Leu Leu Asp Ser Leu Ile Tyr Leu Met Pro Ile Thr His
850 855 860
Pro Val Lys Ser Ile Ile Met Val Ile Ile Asn Asp Gly Arg Gly Ser
865 870 875 880
Ala Ser Asp Cys Val Arg Arg Ala Thr Arg Arg Leu Arg Tyr Ile Gln
885 890 895
Arg His Ile Arg Gly Ser Val Val Val Ser Val Leu Arg Asp Gly Val
900 905 910
Ile Asn Thr Tyr Ser Glu Gly Ile Ala Lys Ala Met Leu Cys Glu Lys
915 920 925
Ser Val Leu Lys Tyr Asn Tyr Lys Val Val Leu Val Lys Val Lys Gly
930 935 940
Tyr Val Phe Gly His Asp Glu Met Leu Thr Lys Leu Leu Asn Val
945 950 955
<210> 150
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV7 CAE53020.1的VP5
<400> 150
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Val Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Arg Ala Ala Glu Arg Phe Ala Glu Ser Glu Val Gly Ala Ala Thr Ile
35 40 45
Asp Gly Ile Val Gln Gly Ser Val His Ser Ile Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ser
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Thr Lys Leu Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Leu Asn Lys Lys Ile Val Asp Lys Phe Gly Thr Glu Leu Glu
115 120 125
Glu Val Tyr Asp Phe Met Asn Gly Ala Ala Lys Asp Asp Glu Asn Glu
130 135 140
Ile Lys Gln Tyr Asp Ile Leu Cys Lys Ala Val Asp Ser Tyr Gly Lys
145 150 155 160
Ile Leu Arg Glu Glu Asp Lys Lys Met Leu Glu Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ser Glu Ser Arg Ser Asp Glu Glu Thr Lys Met Val Arg
180 185 190
Glu Tyr Arg Ser Lys Ile Asp Ala Leu Arg Asp Ala Val Asp Val Glu
195 200 205
Arg Glu Ser Met Thr Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ser Asp Val Leu Glu Ala Ala Ala Glu Glu Val Pro Leu Val Gly Ser
225 230 235 240
Gly Met Ala Thr Ala Ile Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Thr His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Ser Leu Ile Ala Thr Thr Leu Glu
275 280 285
Tyr Arg Asp Arg Pro Ile Pro Asp Asp Ser Leu Ala Val Ser Val Leu
290 295 300
Glu Lys Arg Arg Ala Ile Glu Glu Asn Lys Asn Glu Ile Glu His Ile
305 310 315 320
Lys Asn Glu Ile Leu Pro Arg Phe Lys Lys Ala Met Glu Glu Glu Lys
325 330 335
Glu Ile Ser Gly Ile Glu Glu Ser Val Ile His Pro Lys Val Met Met
340 345 350
Arg Phe Lys Ile Pro Arg Ser Gln Gln Pro Gln Ile His Ile Tyr Cys
355 360 365
Ser Pro Trp Asp Ser Asp Thr Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Met Asn Glu Ser Phe Phe Ile Gly Phe Asp Leu Glu Leu Asp
385 390 395 400
Leu Val His Phe Glu Asp Leu Thr Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Met Val Val Gly Arg Thr Phe Gln Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Gly Arg Ala Met Met Asp Pro Ser Ser Gly Ala Met His Lys Arg
435 440 445
Arg Met Ser Arg Ser Arg Ser Val His Pro Ile Tyr Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Ser Tyr Ala Thr Leu Lys Ser Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Val Leu Arg Gly Pro Ile His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys His Gly Ile Lys Ile
500 505 510
Leu Asn Asp Lys Ile Asp Leu Thr Ser Leu Leu Arg Asn Ala
515 520 525
<210> 151
<211> 961
<212> PRT
<213> 人工序列
<220>
<223> BTV8 CAM57243.2的VP2
<400> 151
Met Glu Glu Leu Ala Ile Pro Ile Tyr Thr Asn Val Phe Pro Ala Glu
1 5 10 15
Leu Leu Asp Gly Tyr Asp Tyr Ile Ile Asp Val Ser Ser Arg Val Glu
20 25 30
Glu Glu Gly Asp Glu Pro Val Lys Arg His Asp Val Thr Glu Ile Pro
35 40 45
Arg Asn Ser Met Phe Asp Ile Lys Asp Glu His Ile Arg Asp Ala Ile
50 55 60
Ile Tyr Lys Pro Val Asn Asn Asp Gly Tyr Val Leu Pro Arg Val Leu
65 70 75 80
Asp Ile Thr Leu Lys Ala Phe Asp Asp Arg Lys Arg Val Val Leu Asn
85 90 95
Asp Gly His Ser Glu Phe His Thr Lys Thr Asn Trp Val Gln Trp Met
100 105 110
Ile Asp Asp Ala Met Asp Val Gln Pro Leu Lys Val Asp Ile Ala His
115 120 125
Thr Arg Ser Arg Ile Ser His Ala Leu Phe Asn Cys Thr Val Arg Leu
130 135 140
His Ser Lys Lys Ala Asp Thr Ala Ser Tyr His Val Glu Pro Val Glu
145 150 155 160
Ile Glu Ser Trp Gly Cys Asn His Thr Trp Leu Ser Arg Ile His His
165 170 175
Leu Val Asn Val Glu Leu Phe His Cys Ser Gln Glu Ala Ala Tyr Thr
180 185 190
Leu Lys Pro Thr Tyr Lys Ile Ile Ser Asn Ala Glu Arg Ala Ser Thr
195 200 205
Ser Asp Ser Phe Asn Gly Thr Met Ile Glu Leu Gly Arg Asn His Gln
210 215 220
Ile Gln Met Gly Asp Gln Gly Tyr Gln Lys Leu Lys Glu Gly Leu Val
225 230 235 240
Gln Val Arg Ile Glu Gly Lys Thr Pro Leu Val Ile Gln Glu Glu Ile
245 250 255
Thr Ala Leu Asn Lys Ile Arg Glu Gln Trp Ile Ala Arg Asn Phe Asp
260 265 270
Gln Arg Glu Ile Lys Val Leu Asp Leu Cys Arg Leu Leu Ser Thr Ile
275 280 285
Gly Arg Lys Met Cys Asn Thr Glu Glu Glu Pro Lys Asn Glu Ala Asp
290 295 300
Leu Ser Val Lys Phe Gln Met Glu Leu Asp Glu Ile Phe Arg Pro Gly
305 310 315 320
Asn Asn Glu Arg Thr Asn Ile Met Gly Gly Gly Val His Arg Lys Asn
325 330 335
Glu Asp Arg Phe Tyr Val Leu Ile Met Ile Ala Ala Ser Asp Thr Asn
340 345 350
Lys Gly Arg Ile Trp Trp Ser Asn Pro Tyr Pro Cys Leu Arg Gly Ala
355 360 365
Leu Ile Ala Ala Glu Val Gln Leu Gly Asp Val Tyr Asn Leu Leu Arg
370 375 380
Asn Trp Phe Gln Trp Ser Val Arg Pro Thr Tyr Val Pro Tyr Asp Arg
385 390 395 400
Asn Arg Glu Ser Asp Lys Tyr Ile Tyr Ser Arg Ile Asn Leu Phe Asp
405 410 415
Ser Thr Leu Arg Pro Gly Asp Lys Ile Val His Trp Glu Tyr Lys Leu
420 425 430
Leu Asn Glu Val Arg Glu Val Ser Ile Asn Lys Gly Asn Glu Cys Asp
435 440 445
Leu Phe Pro Glu Asp Glu Glu Phe Thr Thr Lys Phe His Glu Ala Arg
450 455 460
Tyr Thr Glu Met Lys Asn Gln Ile Ile Gln Ser Gly Trp Asn Gln Arg
465 470 475 480
Asp Phe Lys Met His Lys Ile Leu Glu Asp Gly Ala Asn Val Leu Thr
485 490 495
Ile Asp Phe Glu Lys Asp Ala His Ile Gly Thr Gly Ser Ala Leu Ser
500 505 510
Leu Pro Asp Tyr Tyr Asn Lys Trp Ile Ile Ala Pro Met Phe Asn Ala
515 520 525
Lys Leu Arg Ile Thr Glu Val Val Ile Gly Thr Ala His Thr Asp Asp
530 535 540
Pro Ala Val Gly Arg Ser Ala Lys Ala Phe Thr His Asp Pro Phe Asp
545 550 555 560
Leu Gln Arg Tyr Cys Leu Ala Arg Tyr Tyr Asp Val Arg Pro Gly Met
565 570 575
Met Gly Arg Ala Leu Ser Lys Gln Gln Asn Met Ser Ser Met Thr Asp
580 585 590
Lys Leu Ser Lys Gln Glu Asp Tyr Ala Gly Ile Val Ser Arg Arg Leu
595 600 605
Glu Tyr Lys Glu Arg Glu Asn Arg Cys Leu Thr Glu Thr Ala Gln Tyr
610 615 620
Val Phe Glu Lys Thr Cys Leu Tyr Val Leu Glu Leu Leu Ser Arg His
625 630 635 640
Thr Met Pro Ser Glu Asp Ser Glu Val Thr Phe Glu His Pro Thr Ile
645 650 655
Asp Pro Ser Val Asp Ile Glu Thr Trp Lys Ile Ile Asp Val Ser Gln
660 665 670
Leu Ile Ile Phe Val Phe Asp Tyr Leu Phe Glu Asn Arg Lys Ile Val
675 680 685
Arg Asp Thr Thr Glu Ala Arg Trp Thr Leu Phe Lys Ile Arg Ser Glu
690 695 700
Val Gly Arg Ala Arg Ile Asp Ala Ile Glu Met Thr Phe Pro Arg Phe
705 710 715 720
Gly Arg Met Leu Arg Asn Ala Ser Gln Ala Lys Ile Asn Gln Asp Ile
725 730 735
Ala Cys Leu Asn Phe Leu Pro Leu Leu Phe Ile Ile Gly Asp Asn Ile
740 745 750
Ser Tyr Ala His Arg Gln Trp Ser Ile Pro Val Leu Leu Tyr Ala His
755 760 765
Asp Ile Arg Ile Ile Pro Leu Glu Val Gly Ala Tyr Asn Asn Arg Phe
770 775 780
Gly Leu Thr Ser Tyr Leu Glu Tyr Met Ala Phe Phe Pro Ser Tyr Ala
785 790 795 800
Thr Arg Val Ala Lys Ile Asp Glu Ser Ile Lys Glu Cys Ala Ile Ala
805 810 815
Met Ala Glu Phe Tyr Met Asn Thr Asp Ile His Ser Gly Ser Val Met
820 825 830
Ser Asn Val Ile Thr Thr Lys Arg Leu Leu Tyr Glu Thr Tyr Leu Ala
835 840 845
Ser Leu Cys Gly Gly Tyr Ser Asp Gly Leu Leu Trp Tyr Leu Pro Ile
850 855 860
Thr His Pro Ser Lys Cys Leu Val Ala Phe Glu Val Ala Asp Asp Val
865 870 875 880
Val Pro Leu Ser Val Arg Arg Glu Arg Ile Leu Ser Arg Phe Pro Leu
885 890 895
Ser Ser Arg His Val Lys Gly Ile Ala Leu Ile Ser Val Asp Arg Asn
900 905 910
Gln Lys Val Ser Val Gln Thr Glu Gly Ile Val Thr His Arg Leu Cys
915 920 925
Lys Lys Asn Leu Leu Lys Tyr Val Cys Asp Val Ile Leu Phe Lys Phe
930 935 940
Ser Gly His Val Phe Gly Asn Asp Glu Met Leu Thr Lys Leu Leu Asn
945 950 955 960
Val
<210> 152
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV8 CAM57247.2的VP5
<400> 152
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Leu Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Met Gly Ser
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Met Gln
85 90 95
Thr Lys Ile Arg Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Ile Arg
100 105 110
Leu Lys Tyr Asn Asp Lys Ile Lys Gln Lys Phe Gly Lys Glu Leu Glu
115 120 125
Glu Val Tyr Glu Phe Met Asn Gly Val Ala Lys Gln Glu Glu Asp Glu
130 135 140
Glu Lys His Tyr Asp Val Leu Lys Lys Ala Val Asn Ser Tyr Asp Lys
145 150 155 160
Ile Leu Thr Glu Glu Glu Lys Gln Met Arg Ile Leu Ala Thr Ala Leu
165 170 175
Gln Lys Glu Val Lys Glu Arg Thr Gly Thr Glu Ala Val Met Val Lys
180 185 190
Glu Tyr Arg Asn Lys Ile Asp Ala Leu Lys Glu Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Thr His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Thr Ile Val Ser Thr Val Leu Asp
275 280 285
His Lys Phe Lys Asp Ile Pro Asp Glu Met Leu Ala Val Ser Val Leu
290 295 300
Ser Lys Asn Arg Ala Ile Glu Glu Asn His Lys Glu Ile Ile His Leu
305 310 315 320
Lys Asn Glu Ile Leu Pro Arg Phe Lys Lys Ala Met Asp Glu Glu Lys
325 330 335
Glu Ile Cys Gly Ile Glu Asp Lys Lys Ile His Pro Lys Val Met Met
340 345 350
Lys Phe Lys Ile Pro Arg Thr Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Ile Ser His
370 375 380
His His Ala Asn Glu Ser Phe Phe Ile Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Leu Val His Tyr Glu Asp Leu Thr Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Ala Ala Val Gly Arg Ser Leu Asn Glu Val Tyr Lys Glu Phe
420 425 430
Leu Asn Leu Ala Ile Asn Asn Thr Tyr Ser Ser Gln Met His Ala Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Leu
450 455 460
His Tyr Asp Ile Ser Phe Ser Thr Leu Arg Ser Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Glu Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Ile Lys His Gly Val Lys Ile
500 505 510
Leu Gly Thr Glu Val Asp Ile Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 153
<211> 955
<212> PRT
<213> 人工序列
<220>
<223> BTV9 AEO19807.1的VP2
<400> 153
Met Asp Glu Phe Gly Ile Pro Ile Tyr Leu Ser Ser Ser Val Pro His
1 5 10 15
Asp Leu Leu Ser Asp Tyr Asp Phe Met Ile Asp Leu Ser Asp Lys Val
20 25 30
Val His Ala Gly Asp Arg His Asp Pro Lys Lys Leu Lys Glu Arg Lys
35 40 45
Ala Ile Asp Val Pro Asp Gly Glu Ala Arg Thr Asp Leu Asn Tyr Asn
50 55 60
Pro Thr Asp Asn Glu Gly Asn Gln Leu Pro Arg Ala Leu Asp Ile Ala
65 70 75 80
Leu Ser Ala Arg Leu Thr Arg Lys Ser Val Arg Asn Asn Asp Gly Leu
85 90 95
Asp Phe Leu Thr Asp Asp Lys Trp Met Glu Trp Met Leu Ser Asp Ala
100 105 110
Met Asp Val Gln Pro Leu Lys Ile Asp Leu Ser Ala Arg Asn Gly Phe
115 120 125
Val Lys Cys Asp Ile Phe Asn Ser Ser Ile Tyr Ile Arg Arg Lys Tyr
130 135 140
Ala Asp Ala Ile Ala Tyr Arg Tyr Thr Ser Leu Glu Asp Glu Ser Lys
145 150 155 160
Gly Cys Asn His Thr Arg Val Cys Met Val Asn His Leu Ile Asn Asn
165 170 175
Gly Leu Tyr Asn Ala Val Gln Glu Cys Ala Tyr Ala Leu Lys Asp Thr
180 185 190
Tyr Val Leu Arg Val His Ser Gln Arg Glu Arg Ile Asp Glu Pro Phe
195 200 205
Glu Pro Gly Arg Pro Lys Ile Gly Ser Leu Gly Arg Asn Ala Arg Ile
210 215 220
Asp Met Ser Asp Pro Gly Tyr Ser Leu Phe Lys Gly Gly Met Leu Gln
225 230 235 240
Ile Thr Val Ser Gly Glu Ile Pro Asn Asp Ile Arg Val Glu Met Glu
245 250 255
Arg Leu Asn Gln Ile Arg Ala Thr Trp Ile Arg Asp Lys Ser Ser Arg
260 265 270
Glu Val Arg Ala Ile Glu Leu Cys Thr Leu Leu Ser Arg Ile Gly Arg
275 280 285
Val Lys Trp Asn Ala Glu Glu Glu Pro Lys Asp Glu Gly Ala Met Ser
290 295 300
Leu Arg Phe Gln Asn Lys Ile Asp Ser Met Phe Leu Ser Glu Asn Thr
305 310 315 320
Glu Lys Thr Asn Ile Met Gln Asn Thr Ser Gly Arg Thr Asp Glu Glu
325 330 335
Arg Phe Tyr Ala Leu Leu Leu Ile Cys Ala Thr Asp Ala Tyr Ser Arg
340 345 350
Arg Ile Trp Arg Ser Asn Pro Tyr Pro Cys Leu Arg Gly Thr Leu Ile
355 360 365
Ala Ala Glu Cys Val Leu Gly Asp Pro Tyr Lys Thr Leu Arg Arg Lys
370 375 380
Phe Asn Trp Ser Val Arg His Ala Ala Asp Lys Ala Leu Glu Asn Asn
385 390 395 400
Ala Tyr Ile Phe Thr Arg Ile Asn Leu Phe Asp Thr Glu Arg Thr Pro
405 410 415
Gly Met Arg Val Val His Trp Thr Gln Glu Leu Thr Met Glu Ser Gln
420 425 430
Thr Thr Trp Asp Glu Gly Tyr Pro Leu Lys Asp Glu Ala Pro Asp Asp
435 440 445
Glu Met His Cys Lys Ile Asp Thr Asn Lys Tyr Lys Glu Met Val Thr
450 455 460
Gln Val Ile Asp Gly Gly Trp Asp Gln Glu Asn Phe Lys Ile His Lys
465 470 475 480
Leu Phe His Asp Asp Gly Asn Ile Phe Leu Met Asp Phe Glu Lys Asp
485 490 495
Ala Lys Leu Thr Ala Gln Ser Glu Val Gln Tyr Pro Asp Tyr Tyr Asp
500 505 510
Lys Trp Ile Tyr Ala Pro Ile Phe Asp Thr Lys Tyr Lys Ile Thr Glu
515 520 525
Thr Glu Ile Ala Asn Ala Lys Asn Leu Asp Pro Ala Ile Lys Arg Thr
530 535 540
Ile Asp Pro Met Met Asp Asp Pro Val Met Leu Gln Leu Ser Thr Ile
545 550 555 560
Gly Asn Leu Tyr Asp Thr Arg Pro Ala Val Met Gly Ser Thr Leu Ser
565 570 575
Met Arg Gln Arg Gln Thr Ser Leu His Lys Thr Leu Leu Asp Asp Pro
580 585 590
Gln Asn Glu Arg Phe Tyr Ser Ser Arg Ser Ile Lys Lys Val Asp Asp
595 600 605
Pro Cys Pro Val Tyr Tyr Thr Ala Ser Leu Val Leu Gln Lys Ile Ser
610 615 620
Glu Leu Val Met Ser Leu Met Ile Tyr His Val Asp Gln Asp Trp Glu
625 630 635 640
Ser Leu Lys Gln Tyr Lys His Pro Ser Ile Leu Ser Asp Phe Ser Ser
645 650 655
Leu Val Ser Asn Ile Gln Asp Ile Ser Gln Leu Val Val Leu Leu Val
660 665 670
Asp Phe Phe Phe Glu Arg Arg Ser Val Leu Arg Ser Val Glu Glu Ser
675 680 685
Arg Tyr Ile Ile His Ile Ile Arg Ser Ser Asn Asp Ala Asn Arg Leu
690 695 700
Lys Ser Phe Glu Leu Tyr Phe Pro Arg Phe Gly Gly Ile Leu Arg Thr
705 710 715 720
Ile Lys Asp Ala Lys Glu Val Ser His Val Thr Ala Leu Asn Phe Leu
725 730 735
Pro Tyr Phe Phe Leu Leu Gly Asp Asn Ile Ile Tyr Lys His Lys Glu
740 745 750
Trp Ser Val Pro Val Leu Ile Tyr Ser Asp Gly Leu Met Ile Trp Pro
755 760 765
Ala Gln Val Gly Ala Asn Phe Asn Arg Phe Gly Phe Phe Gly Phe Leu
770 775 780
Asn Tyr Met Arg Phe His Pro Gly Ile Arg Leu Arg Ser Lys Val Leu
785 790 795 800
Asp Asp Ala Glu Lys Val Val Ala Val Glu Met Leu Asn Tyr Tyr Met
805 810 815
Arg Thr Asp Ile Phe Lys Gly Gly Ile Gln Lys Asn Val Arg Val Thr
820 825 830
Lys Leu Ser Gln Met Glu Ile Tyr His Ser Ser Leu Cys Gly Gly Leu
835 840 845
Ser Asp Ala Leu Ile Tyr Ala Leu Pro Ile Ser Phe Pro Ile Lys Cys
850 855 860
Leu Thr Leu Ile Ile Val Gly Asp Asp Leu Ala Glu Pro Gln His Arg
865 870 875 880
Ile Ser Lys Ala Leu Glu Phe Phe Glu His Val Arg Asp His Val Arg
885 890 895
Gly Ile Ala Ser Ile Ser Ile Ser Arg Asn Gly Asp Val Thr Thr His
900 905 910
Ser Gln Gly Ile Val Lys Val Glu Leu Leu Lys Lys Asn Ile Leu Arg
915 920 925
His Gln Phe Arg Val Ala Leu Leu Lys Val Lys Gly Tyr Val Phe Gly
930 935 940
Asn Asp Glu Met Leu Thr Lys Leu Leu Asn Val
945 950 955
<210> 154
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV9 CAE53024.1的VP5
<400> 154
Met Gly Lys Val Ile Arg Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ser
65 70 75 80
Gly Glu Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Thr Lys Leu Arg Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Asp Glu Ile Lys Arg Lys Phe Gly Glu Glu Leu Glu
115 120 125
Gln Val Tyr Asp Phe Met Asn Gly Val Ala Lys Asp Glu Glu Gly Glu
130 135 140
Glu Lys Gln Phe Asp Ile Leu Asn Lys Ala Val Asn Ser Tyr Asp Lys
145 150 155 160
Ile Leu Ala Glu Glu Asp Thr Gln Met Arg Arg Leu Ala Thr Ala Leu
165 170 175
Gln Lys Glu Ile Gly Asp Arg Thr His Thr Glu Thr Thr Met Val Lys
180 185 190
Glu Tyr Arg Ser Lys Ile Glu Ala Leu Lys Asn Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Thr His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Ser Val Val Ser Thr Ile Leu Glu
275 280 285
Tyr Arg Ala Arg Asp Val Pro Asp Lys Ala Leu Ala Val Ser Val Leu
290 295 300
Ser Lys Asn Arg Thr Ile Gln Glu Asn His Lys Glu Leu Ile His Ile
305 310 315 320
Lys Asp Glu Ile Leu Pro Arg Phe Lys Lys Ala Met Glu Glu Glu Lys
325 330 335
Glu Ile Cys Gly Ile Glu Asp Lys Thr Ile His Pro Lys Val Met Met
340 345 350
Lys Phe Lys Ile Pro Arg Thr Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Ile Ser His
370 375 380
His His Ala Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Ser Ile Asp
385 390 395 400
Leu Val His Tyr Glu Asp Leu Thr Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Thr Ala Ala Gly Arg Thr Leu Thr Glu Ala Tyr Lys Glu Phe
420 425 430
Leu Asn Leu Ala Ile Ser Asn Asn Tyr Gly Thr Gln Met His Thr Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Leu
450 455 460
His Tyr Asp Val Ser Phe Ala Asp Leu Arg Gly Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Cys Lys Ile
500 505 510
Leu Gly Asp Gln Ile Asp Ile Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 155
<211> 955
<212> PRT
<213> 人工序列
<220>
<223> BTV9 CAE51096.1的VP2
<400> 155
Met Asp Glu Phe Gly Ile Pro Leu Tyr Arg Ser Thr His Val Pro His
1 5 10 15
His Val Leu Glu Asp Tyr Glu Leu Arg Ile Asp Leu Ser Asp Arg Val
20 25 30
Val His Gly Gly Asp Arg His Asp Pro Lys Lys Leu Lys Glu Arg Lys
35 40 45
Ala Ile Asp Val Pro Asp Gly Asp Val Arg Leu Asp Leu Asn Tyr Asn
50 55 60
Pro Thr Asp Asn Glu Gly Asn Gln Ile Pro Gly Ala Leu Asp Ile Ala
65 70 75 80
Leu Cys Ala Arg Ile Thr Arg Lys Ser Val Lys Thr Asn Glu Gly Val
85 90 95
Thr Phe Thr Thr Asn His Lys Trp Met Glu Trp Met Leu Leu Asp Ala
100 105 110
Met Asp Val Gln Pro Leu Lys Ile Asp Phe Ala Ala Leu Gly Gly Ala
115 120 125
Val Lys Cys Asp Leu Phe Asn Ser Ala Ile Tyr Val Lys Lys Lys His
130 135 140
Ala Asp Ala Ile Met Tyr Arg Tyr Ile Ala Val Glu Asp Glu Ser Arg
145 150 155 160
Gly Cys Asn His Ser Arg Val Gln Asp Val Asn His Leu Ile Asn Asn
165 170 175
Gly Leu Tyr Asn Val Ala Gln Glu Cys Ala Tyr Ala Phe Lys Asp Thr
180 185 190
Tyr Val Leu Arg Val His Ser Gln Arg Glu Asn Val Glu Glu Pro Phe
195 200 205
Glu Ile Gly Arg Pro Lys Val Gly Ser Leu Gly Arg Asn Ala Val Ile
210 215 220
Ser Met Glu Asn Pro Gly Tyr Pro Leu Phe Arg Ala Gly Leu Leu Gln
225 230 235 240
Ile Thr Val Ser Gly Thr Ile Pro Ala Asp Ile Asn Asp Glu Met Asn
245 250 255
Arg Leu Asn Gln Ile Arg Ser Ala Trp Ile Arg Asp Lys Ala Thr Arg
260 265 270
Glu Val Lys Ala Ile Glu Leu Cys Thr Leu Leu Ser Arg Ile Gly Arg
275 280 285
Leu Lys Met Asp Met Glu Glu Glu Pro Lys Asp Glu Gly Ala Met Ser
290 295 300
Leu Arg Phe Gln Gly Lys Ile Asp Thr Met Phe Phe Ser Glu Asn Thr
305 310 315 320
Glu Lys Thr Asn Ile Met Arg Asn Ile Ser Gly Arg Thr Asp Glu Glu
325 330 335
Arg Phe Tyr Val Leu Leu Leu Ile Cys Ala Thr Asp Ala Tyr Thr Arg
340 345 350
Arg Ile Trp Arg Ser Asn Pro Tyr Pro Cys Leu Arg Gly Thr Leu Ile
355 360 365
Ala Thr Glu Cys Val Leu Gly Asp Ala Tyr Lys Thr Leu Arg Gln Lys
370 375 380
Phe Asp Trp Ser Val Arg His Ala Ala Asp Lys Ala Ile Glu Asn Phe
385 390 395 400
Ser His Ile Phe Thr Arg Ile Asn Leu Phe Asp Thr Gly Lys Thr Pro
405 410 415
Gly Ala Arg Ile Ile His Trp Val Gln Glu Met Thr Met Gly Thr Lys
420 425 430
Thr Ser Trp Glu Ser Gly Tyr Pro Leu Lys Asp Glu Ala Pro Asp Asp
435 440 445
Glu Leu His Cys Arg Val Asp Arg Asp Gln His Arg Gln Met Val Thr
450 455 460
Arg Ile Ile Asp Gly Gly Trp Asp His Glu Asn Phe Lys Ile His Lys
465 470 475 480
Leu Phe His Glu Gln Gly Asn Ile Phe Leu Met Asp Phe Glu Lys Asp
485 490 495
Ala Arg Leu Thr Ser Arg Ser Glu Val Glu Tyr Pro Tyr Tyr Tyr Asp
500 505 510
Arg Trp Ile Tyr Ala Ser Ile Phe Asp Thr Arg Tyr Lys Ile Met Glu
515 520 525
Thr Glu Ile Ala Thr Ala Lys Asn Ala Asp Pro Ala Ile Lys Arg Thr
530 535 540
Leu Ala Pro Leu Met Asp Asp Pro Val Met Leu Gln Leu Arg Thr Ile
545 550 555 560
Gly Asn Leu Tyr Asp Thr Arg Pro Ala Ile Met Gly Ser Thr Leu Ser
565 570 575
Ala Arg Gln Ala Gln Thr Ser Leu His Lys Thr Leu Leu Asp Glu Ala
580 585 590
Glu Asn Gly Arg Tyr Tyr Arg Ser Arg Gly Leu Glu Lys Arg Ala Asn
595 600 605
Pro Cys Ser Val Cys Tyr Thr Ser Ser Phe Val Leu Gln Lys Val Ser
610 615 620
Glu Leu Val Leu Ser Leu Met Ile Tyr His Val Asp His Asp Trp Glu
625 630 635 640
Pro Leu Lys Gln Tyr Asn His Pro Asp Val Leu Ser Glu Phe Ser Glu
645 650 655
Thr Val Arg Glu Ile His Asp Ile Ser Gln Leu Ile Val Leu Leu Val
660 665 670
Asp Leu Phe Phe Glu Gln Arg Arg Thr Val Arg Pro Val Glu Glu Ala
675 680 685
Arg Tyr Val Ile Tyr Ser Ile Arg Val Ala Arg Gly Ala Asp Arg Leu
690 695 700
Asp Thr Phe Arg Arg Phe Phe Pro Ala Phe Gly Arg Ala Leu Asp Val
705 710 715 720
Val Asn Arg Ala Thr Asp Val Ala His Ile Thr Ala Leu Asn Phe Leu
725 730 735
Pro Phe Phe Phe Leu Leu Gly Asp Asn Ile Ile Tyr Lys His Arg Glu
740 745 750
Trp Thr Val Pro Val Leu Ile Tyr Thr Glu Gly Val Met Ile Trp Pro
755 760 765
Ala Gln Val Gly Ala Asn Phe Asn Arg Phe Gly Phe Cys Gly Phe Leu
770 775 780
Asn Tyr Met Arg Phe His Ala Gly Ser Arg Leu Arg Ala Lys Pro Leu
785 790 795 800
Asp Asp Ala Glu Lys Ile Val Thr Arg Glu Met Leu Arg Tyr Tyr Met
805 810 815
Lys Thr Asp Ile Phe Asp Gly Gly Val Gln Lys Asn Ile Arg Val Thr
820 825 830
Lys Ser Ser Gln Met Glu Ile Tyr His Ser Ser Leu Cys Gly Gly Leu
835 840 845
Ser Asp Ala Val Val Tyr Val Leu Pro Ile Ser Phe Pro Ile Lys Cys
850 855 860
Leu Thr Leu Ile Ile Val Gly Asp Asp Leu Val Glu Pro Gln Met Arg
865 870 875 880
Phe Gly Arg Val Met Asp Phe Phe Arg His Val Ala Glu His Val Arg
885 890 895
Gly Val Ala Ser Ile Ser Ile Ser Arg Asn Gly Asp Ile Thr Thr His
900 905 910
Ser Gln Gly Ile Val Arg Val Glu Leu Leu Lys Lys Asn Ile Leu Lys
915 920 925
Tyr Gln Phe Gln Val Ala Leu Leu Lys Val Lys Gly Phe Val Phe Gly
930 935 940
Asn Asp Glu Ile Leu Ile Lys Leu Leu Asn Val
945 950 955
<210> 156
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV9 CAE53024.1的VP5
<400> 156
Met Gly Lys Val Ile Arg Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ser
65 70 75 80
Gly Glu Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Thr Lys Leu Arg Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Asp Glu Ile Lys Arg Lys Phe Gly Glu Glu Leu Glu
115 120 125
Gln Val Tyr Asp Phe Met Asn Gly Val Ala Lys Asp Glu Glu Gly Glu
130 135 140
Glu Lys Gln Phe Asp Ile Leu Asn Lys Ala Val Asn Ser Tyr Asp Lys
145 150 155 160
Ile Leu Ala Glu Glu Asp Thr Gln Met Arg Arg Leu Ala Thr Ala Leu
165 170 175
Gln Lys Glu Ile Gly Asp Arg Thr His Thr Glu Thr Thr Met Val Lys
180 185 190
Glu Tyr Arg Ser Lys Ile Glu Ala Leu Lys Asn Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Thr His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Ser Val Val Ser Thr Ile Leu Glu
275 280 285
Tyr Arg Ala Arg Asp Val Pro Asp Lys Ala Leu Ala Val Ser Val Leu
290 295 300
Ser Lys Asn Arg Thr Ile Gln Glu Asn His Lys Glu Leu Ile His Ile
305 310 315 320
Lys Asp Glu Ile Leu Pro Arg Phe Lys Lys Ala Met Glu Glu Glu Lys
325 330 335
Glu Ile Cys Gly Ile Glu Asp Lys Thr Ile His Pro Lys Val Met Met
340 345 350
Lys Phe Lys Ile Pro Arg Thr Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Ile Ser His
370 375 380
His His Ala Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Ser Ile Asp
385 390 395 400
Leu Val His Tyr Glu Asp Leu Thr Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Thr Ala Ala Gly Arg Thr Leu Thr Glu Ala Tyr Lys Glu Phe
420 425 430
Leu Asn Leu Ala Ile Ser Asn Asn Tyr Gly Thr Gln Met His Thr Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Leu
450 455 460
His Tyr Asp Val Ser Phe Ala Asp Leu Arg Gly Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Cys Lys Ile
500 505 510
Leu Gly Asp Gln Ile Asp Ile Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 157
<211> 956
<212> PRT
<213> 人工序列
<220>
<223> BTV10 CAE51097.1的VP2
<400> 157
Met Glu Glu Phe Val Ile Pro Val Phe Ser Glu Arg Asp Ile Pro Tyr
1 5 10 15
Ser Leu Leu Asn His Tyr Pro Leu Ala Ile Arg Ile Asp Val Lys Val
20 25 30
Asp Asp Glu Asp Gly Arg His Asn Leu Ile Lys Ile Pro Glu Ser Asp
35 40 45
Met Ile Asp Val Pro Lys Leu Ser Val Ile Glu Ala Leu Asn Tyr Arg
50 55 60
Pro Lys Arg Asn Asp Gly Val Val Val Pro Arg Leu Leu Asp Ile Thr
65 70 75 80
Leu His Ala Tyr Asp Lys Arg Lys Ser Thr Lys Ser Ala Lys Gly Val
85 90 95
Glu Phe Thr Thr Asp Ala Lys Trp Met Lys Trp Ala Ile Asp Asp Lys
100 105 110
Met Asp Ile Gln Pro Leu Lys Val Thr Leu Asp Asn His Tyr Ser Val
115 120 125
Asn His Gln Leu Phe Asn Cys Ile Val Lys Ala Arg Ser Ala Asn Ala
130 135 140
Asp Thr Ile Tyr Tyr Asp Tyr Tyr Pro Leu Glu Asn Gly Ala Lys Lys
145 150 155 160
Cys Asn His Thr Asn Leu Asp Leu Leu Arg Ser Leu Thr Thr Thr Glu
165 170 175
Met Phe His Ile Leu Gln Gly Ala Ala Tyr Ala Leu Lys Thr Thr His
180 185 190
Glu Leu Val Ala His Ser Glu Arg Glu Ser Thr Ser Glu Thr Tyr Gln
195 200 205
Val Gly Thr Gln Arg Trp Ile Gln Leu Arg Lys Gly Thr Lys Ile Gly
210 215 220
Tyr Arg Gly Gln Pro Tyr Glu Arg Phe Ile Ser Ser Leu Val Gln Val
225 230 235 240
Ile Ile Lys Gly Arg Val Pro Asp Glu Ile Arg Asp Glu Ile Ala Glu
245 250 255
Leu Asn Arg Ile Lys Asp Glu Trp Lys Asn Ala Ala Tyr Asp Arg Thr
260 265 270
Lys Ile Arg Ala Leu Glu Leu Cys Lys Ile Leu Ser Ala Ile Gly Arg
275 280 285
Lys Met Leu Asp Val Gln Glu Glu Pro Lys Asp Glu Met Ala Leu Ser
290 295 300
Thr Arg Phe Gln Phe Lys Leu Asp Glu Lys Phe Ile Arg Thr Asp Gln
305 310 315 320
Glu His Val Asn Ile Phe Glu Val Gly Gly Pro Ala Thr Asp Asp Gly
325 330 335
Arg Phe Tyr Ala Leu Ile Ala Ile Ala Ala Thr Asp Thr Gln Gln Gly
340 345 350
Arg Val Trp Arg Thr Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu Ile
355 360 365
Ala Ala Glu Cys Glu Leu Gly Asp Val Tyr Phe Thr Leu Arg Gln Thr
370 375 380
Tyr Lys Trp Ser Leu Arg Ser Glu Tyr Gly Gln Arg Glu Arg Pro Leu
385 390 395 400
Glu Asp Asn Lys Tyr Val Phe Ser Arg Leu Asn Leu Phe Asp Thr Asn
405 410 415
Leu Ala Ile Gly Asp Glu Ile Ile His Trp Arg Tyr Glu Ile Tyr Arg
420 425 430
Pro Lys Glu Thr Thr His Asp Asp Gly Tyr Ile Cys Val Ser Gln Lys
435 440 445
Asp Asp Asp Glu Leu Leu Cys Glu Ile Asp Gly Asp Arg Tyr Lys Glu
450 455 460
Met Phe Asp Arg Met Ile Gln Gly Gly Trp Asp Gln Glu Arg Phe Lys
465 470 475 480
Leu His Asn Ile Leu Thr Glu Pro Asn Leu Leu Thr Ile Asp Phe Glu
485 490 495
Lys Asp Ala Tyr Leu Gly Ser Arg Ser Glu Leu Val Phe Pro Pro Tyr
500 505 510
Tyr Asp Lys Trp Ile Asn Ser Pro Met Phe Asn Ala Lys Leu Lys Ile
515 520 525
Ala Arg Gly Glu Ile Ala Thr Arg Lys Val Asp Asp Pro Trp Asn Asn
530 535 540
Arg Ala Val His Gly Tyr Ile Lys Thr Ser Ala Glu Ser Leu Gly Tyr
545 550 555 560
Val Leu Gly Pro Tyr Tyr Asp Leu Arg Leu Gln Leu Phe Gly Asp Ala
565 570 575
Leu Ser Leu Glu Gln Lys Gln Ser Ala Val Phe Glu Tyr Met Ala Gln
580 585 590
Gln Asp Asp Phe Pro Ala Leu Thr Asp Tyr Thr Lys Glu Lys Asn Gly
595 600 605
Cys Pro His Ser Gly Gly Thr Phe Tyr Thr Phe Arg Lys Val Ala Leu
610 615 620
Ile Ile Leu Ser Ser Tyr Glu Arg Leu Asp Pro Ser Leu His Glu Gly
625 630 635 640
Arg Glu His Glu Thr Tyr Met His Pro Ala Ile Asn Asp Val Phe Arg
645 650 655
Arg Tyr Ala Leu Glu Met Lys Glu Phe Ser Gln Leu Ile Cys Phe Val
660 665 670
Phe Asp Tyr Ile Phe Glu Lys His Val Gln Leu Arg Asn Ala Lys Glu
675 680 685
Ala Arg Arg Ile Ile Tyr Leu Ile Gln Asn Thr Ser Gly Val His Arg
690 695 700
Leu Asp Ile Leu Arg Glu Thr Phe Pro Asn Phe Leu Arg His Val Met
705 710 715 720
Asn Leu Arg Asp Val Lys Arg Ile Cys Asp Leu Asn Val Ile Asn Phe
725 730 735
Phe Pro Leu Leu Phe Leu Ile Gln Asp Asn Ile Ser Tyr Trp His Arg
740 745 750
Gln Trp Ser Ile Pro Met Ile Leu Phe Gly Glu Val Val Arg Leu Ile
755 760 765
Pro Ile Glu Val Gly Ala Tyr Ala Asn Arg Phe Gly Phe Lys Ser Phe
770 775 780
Leu Asn Phe Ile Arg Phe His Pro Gly Asp Ser Lys Lys Lys Gln Asp
785 790 795 800
Ala Asp Asp Thr His Lys Glu Phe Gly Ser Ile Cys Phe Glu Tyr Tyr
805 810 815
Thr Thr Thr Lys Ile Ser Gln Gly Glu Ile Asp Val Pro Val Ile Thr
820 825 830
Ser Lys Leu Asp Thr Leu Lys Leu His Ile Ala Ser Leu Cys Ala Gly
835 840 845
Leu Ala Asp Ser Leu Val Tyr Thr Leu Pro Val Ala His Pro Lys Lys
850 855 860
Ser Ile Val Leu Ile Ile Val Gly Asp Asp Lys Leu Glu Pro Gln Val
865 870 875 880
Arg Ser Glu Gln Ile Val Asn Lys Tyr His Tyr Ser Arg Arg His Ile
885 890 895
Ser Gly Val Val Ser Ile Cys Val Asp Gln Asn Gly Gln Leu Lys Val
900 905 910
His Ser Met Gly Ile Thr Arg His Arg Ile Cys Asp Lys Ser Ile Leu
915 920 925
Lys Tyr Lys Cys Lys Val Val Leu Val Arg Met Pro Gly His Val Phe
930 935 940
Gly Asn Asp Glu Leu Met Thr Lys Leu Leu Asn Val
945 950 955
<210> 158
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV10 CAE53025.1的VP5
<400> 158
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Val Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ala Ala Thr Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Thr
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Met Gln
85 90 95
Met Lys Ile Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Lys Glu Ile Thr Glu Lys Phe Gly Lys Glu Leu Gly
115 120 125
Glu Val Tyr Asp Phe Met Asn Gly Glu Ala Lys Glu Val Glu Ala Val
130 135 140
Glu Glu Gln Tyr Thr Met Leu Cys Lys Ala Val Asp Ser Tyr Glu Lys
145 150 155 160
Ile Leu Lys Glu Glu Asp Ser Lys Met Ala Ile Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Ala Glu Arg Ser Glu Asp Glu Ile Lys Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Ser Ala Ile Glu Ile Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Met Arg Ser Pro Lys Ile Glu Pro Thr Ile Ile Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Lys Asp Ile Pro Asp Glu Gln Leu Ala Val Ser Val Leu
290 295 300
Asn Lys Lys Thr Ala Val Ala Asp Asn Cys Ser Glu Ile Ala His Ile
305 310 315 320
Lys Gln Glu Ile Leu Pro Lys Phe Lys Gln Ile Met Asn Glu Glu Lys
325 330 335
Glu Ile Glu Gly Ile Glu Asp Lys Val Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Ile Pro Arg Thr Gln Gln Pro Gln Ile His Ile Tyr Ala
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Arg Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Thr Ser His Trp His Ala Leu Gly Met
405 410 415
Ala Gln Glu Ala Ser Gly Arg Thr Leu Thr Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ser Ile Ser Ser Thr Phe Ser Ser Ala Ile His Ala Arg
435 440 445
Arg Met Ile Arg Ser Arg Ala Val His Pro Ile Phe Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Ala Leu Lys Asn Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Ile
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 159
<211> 956
<212> PRT
<213> 人工序列
<220>
<223> BTV11 CAE51098.1的VP2
<400> 159
Met Glu Glu Phe Val Ile Pro Val Tyr Ser Glu Thr Asp Ile Pro Tyr
1 5 10 15
Ser Leu Leu Ser His Tyr Pro Leu Ala Ile Arg Thr Asp Val Lys Ile
20 25 30
Ala Asp Thr Asp Glu Gly His Asp Val Val Lys Ile Pro Glu Ser Asp
35 40 45
Met Ile Asp Val Pro Arg Val Asp Ile Val Glu Ala Leu Ala Ala Lys
50 55 60
Pro Met Arg Asn Asp Gly Ile Val Val Pro Arg Leu Leu Asp Ile Thr
65 70 75 80
Leu Arg Ala Tyr Asp Asp Arg Lys Ala Ile Lys Ser Ala Arg Gly Val
85 90 95
Glu Phe Met Thr Asn Ala Lys Trp Met Lys Trp Ala Ile Asp Asp Arg
100 105 110
Met Asp Ile Gln Pro Leu Lys Val Ala Ile Asp His Tyr Asn Ala Val
115 120 125
Asn His Gln Leu Phe Asn Cys Ile Val Lys Ala Arg Ser Ala Asn Ala
130 135 140
Asp Thr Ile Tyr Tyr Asp Tyr Phe Pro Leu Glu Ser Lys Val Lys Lys
145 150 155 160
Cys Asn His Ala Asn Leu Asp Leu Leu Arg Ser Leu Thr Asn Thr Glu
165 170 175
Met Phe His Met Leu Gln Gly Ala Ala Tyr Ser Leu Lys Ser Asn Tyr
180 185 190
Glu Leu Ile Thr Asn Ser Glu Arg Asn Ser Thr Glu Glu Thr Tyr Ala
195 200 205
Pro Gly Val His Asn Gln Ile Arg Leu Val Arg Gly Thr Arg Ile Gly
210 215 220
Tyr Lys Gly Glu Ala Tyr Ser Arg Phe Val Ser Ser Leu Val Gln Val
225 230 235 240
Arg Ile Gln Gly Arg Thr Pro Pro Glu Ile Val Asp Asp Ile Ala Arg
245 250 255
Leu Asn Val Ile Arg Thr Glu Trp Ile Asn Ala Gln Phe Asp Ser Thr
260 265 270
Lys Ile Arg Ala Leu Glu Leu Cys Lys Ile Leu Ser Ala Ile Gly Arg
275 280 285
Lys Met Leu Asn Thr His Glu Glu Pro Lys Asp Glu Met Asp Leu Ser
290 295 300
Thr Lys Phe Gln Phe Lys Leu Asp Asp Lys Phe Lys Lys Thr Asp Ser
305 310 315 320
Glu His Ile Asn Ile Phe Asn Val Arg Ala Pro Ala Thr His Glu Gly
325 330 335
Arg Phe Tyr Ala Leu Ile Ala Ile Ala Ala Thr Asp Thr Gln Arg Gly
340 345 350
Arg Ile Trp Arg Thr Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu Ile
355 360 365
Ala Ala Glu Cys Glu Leu Gly Asp Val Tyr His Thr Leu Arg Gln Val
370 375 380
Tyr Lys Trp Ser Leu Arg Gln Asp Tyr Gly Arg Thr Glu Val Pro Leu
385 390 395 400
Glu Asn Asn Lys Tyr Val Phe Ser Arg Ile Asn Leu Phe Asp Ser Asn
405 410 415
Leu Asp Val Gly Asp Gln Val Val His Trp Met Tyr Glu Val Asp Gly
420 425 430
Pro Ala Glu Thr Thr Tyr Asp Asn Gly Tyr Met Cys Lys Thr Glu Arg
435 440 445
Glu Asp Glu Glu Leu Val Cys Lys Ile Ser Glu Glu Lys Tyr Lys Thr
450 455 460
Met Leu Asp Arg Met Ile Gln Gly Gly Trp Asp Gln Glu Arg Phe Lys
465 470 475 480
Leu Tyr Ser Ile Leu Thr Asp Pro Asn Leu Leu Thr Ile Asp Phe Glu
485 490 495
Lys Asp Ala His Leu Asn Ile Arg Ser Glu Phe Val Leu Pro Ser Tyr
500 505 510
Phe Asp Gln Trp Ile Tyr Ser Pro Met Phe Asn Ala Arg Leu Arg Ile
515 520 525
Thr His Gly Glu Ile Ala Thr Arg Lys Ser Ala Asp Pro Trp Asn Lys
530 535 540
Arg Val Val Phe Gly Tyr Ile Lys Ala Ser Thr Glu Ser Pro Glu Tyr
545 550 555 560
Ala Leu Gly Gln Tyr Phe Asp Met Arg Ile Gln Phe Tyr Gly Asp Ala
565 570 575
Leu Ser Ser Lys Gln Asn Gln Ser Ala Val Phe Gln Tyr Leu Ser Gln
580 585 590
Gln Glu Asp Phe Pro Thr Leu Thr Ser Tyr Ala Lys Gly Asp Val Val
595 600 605
Cys Pro His Ser Gly Gly Ala Leu Tyr Thr Phe Arg Arg Val Ala Leu
610 615 620
Met Leu Met Ala Asn Tyr Glu Lys Leu Ser Pro Asp Leu His Glu Gly
625 630 635 640
Met Glu Asp Tyr Thr Tyr Thr His Pro Ser Ile Gly Gly Ala Tyr Gln
645 650 655
Lys Arg Ile Leu Glu Met Arg Asp Phe Ser Gln Leu Ile Cys Phe Ile
660 665 670
Ile Asp Tyr Ile Phe Glu Arg His Asp Gln Leu Arg Asp Ala Lys Glu
675 680 685
Ala Arg Arg Ile Leu Tyr Leu Ile Gln Asn Leu Asp Glu Pro Gln Arg
690 695 700
Leu Glu Met Leu Asn Val Thr Phe Pro Asn Phe Phe Arg His Phe Leu
705 710 715 720
Lys Leu Lys Asp Val Gln Leu Ile Ser Asp Leu Asn Val Ile Asn Phe
725 730 735
Leu Pro Leu Leu Phe Leu Val Gln Asp Asn Ile Ser Tyr Trp His Arg
740 745 750
Gln Trp Ala Val Pro Met Ile Leu Tyr Asp Asp Val Ile Arg Leu Ile
755 760 765
Pro Val Glu Val Gly Ala Tyr Ala Asn Arg Phe Gly Ile Lys Ser Phe
770 775 780
Phe Asn Phe Thr Arg Phe His Pro Gly Asp Ala Lys Lys Arg Gln Lys
785 790 795 800
Ala Asp Asp Thr His Lys Glu Phe Gly Ser Ile Ser Phe Asn Tyr Tyr
805 810 815
Ala Ser Thr Lys Ile Ala Gln Gly Gly Val His Thr Pro Val Val Thr
820 825 830
Thr Lys Leu Asp Thr Leu Lys Ile His Leu Ser Ser Leu Cys Ala Gly
835 840 845
Leu Ala Asp Ser Ile Val Tyr Thr Leu Pro Val Ala His Pro Lys Lys
850 855 860
Cys Ile Val Leu Ile Ile Val Gly Asp Asp Lys Leu Glu Pro His Val
865 870 875 880
Arg Ser Glu Gln Val Val Ser Lys Tyr Tyr Phe Ser Arg Arg His Val
885 890 895
Ser Gly Ile Val Ser Ile Cys Val Gly Gln Asp Asn Gln Leu Lys Val
900 905 910
Tyr Ser Ser Gly Ile Val Arg His Arg Val Cys Glu Lys Phe Ile Leu
915 920 925
Lys Tyr Lys Cys Lys Val Val Leu Val Lys Met Pro Gly Tyr Val Phe
930 935 940
Gly Asn Asp Glu Leu Met Thr Lys Leu Leu Asn Val
945 950 955
<210> 160
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV11 CAE53026.1的VP5
<400> 160
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Ser Ala Leu Ser Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ala Ala Thr Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Thr
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Met Gln
85 90 95
Thr Lys Ile Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Lys Glu Ile Thr Lys Glu Phe Gly Lys Glu Leu Glu
115 120 125
Glu Val Tyr Asp Phe Met Asn Gly Glu Ala Lys Glu Glu Glu Val Val
130 135 140
Gln Glu Gln Tyr Ser Met Leu Cys Lys Ala Val Asp Ser Tyr Glu Lys
145 150 155 160
Ile Leu Lys Ala Glu Asp Ser Lys Met Ala Ile Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Ser Glu Arg Ser Gln Asp Glu Ile Lys Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Asn Ala Ile Glu Ile Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Met Arg Ser Pro Lys Ile Glu Pro Thr Ile Ile Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Lys Glu Ile Pro Asp Glu Gln Leu Ala Val Ser Val Leu
290 295 300
Asn Lys Lys Thr Ala Val Thr Asp Asn Cys Asn Glu Ile Ala His Ile
305 310 315 320
Lys Gln Glu Ile Leu Pro Lys Phe Lys Gln Ile Met Asp Glu Glu Lys
325 330 335
Glu Ile Glu Gly Ile Glu Asp Lys Val Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Ile Pro Arg Thr Gln Gln Pro Gln Ile His Ile Tyr Ala
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Arg Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Thr Ser His Trp His Ala Leu Gly Leu
405 410 415
Ala Gln Glu Ala Ser Gly Arg Thr Leu Thr Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ser Ile Ser Ser Thr Tyr Ser Ser Ala Ile His Ala Arg
435 440 445
Arg Met Ile Arg Ser Arg Ala Val His Pro Ile Phe Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Ala Leu Lys Asn Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Glu Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Ile
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 161
<211> 950
<212> PRT
<213> 人工序列
<220>
<223> BTV12 CAE51099.1的VP2
<400> 161
Met Glu Asp Phe Pro Ile Cys Val Ile Glu Arg Glu Ser Lys Phe Asp
1 5 10 15
Glu Asp Phe Val Val Arg Tyr Pro Val Ile Ile Asp Leu Lys Glu Cys
20 25 30
Val Asp Asp Val Asn Gly Lys Thr Asp Val Ser Lys Leu Glu Glu Thr
35 40 45
Arg Gly Ile Phe Val Arg Ala Glu Gly Ile Arg Val Ala Leu Asn Ser
50 55 60
Arg Pro Asp Asn Leu Ser His Val Ile Thr Pro Ser Ala Leu Asp Val
65 70 75 80
Leu Ile Arg Ala Tyr Asp Arg Lys His Lys Ile Ala Leu Asp Lys Arg
85 90 95
Thr Asp Asp Ser Gly Arg Met Ala Asp His Asn Ile Asp Val Trp Val
100 105 110
Lys Lys Arg Leu Glu Glu Gln Ala Asp Leu Gln Ala Ile Tyr His Asp
115 120 125
Val Asp Leu Gly Arg Gly Lys Leu Lys Phe Lys Met Leu Thr Gly Leu
130 135 140
Val Lys Leu Asp Ser Asn Tyr Ala Glu Thr Val Gly Tyr Asp Tyr Ala
145 150 155 160
Pro Val Pro Lys Val Gly Cys Asp His Gly Asn Val Asp Phe Leu Tyr
165 170 175
Asp Phe Ile Leu Ser Gly Ser Leu Gln Val Ser Lys Thr Ala Gly Tyr
180 185 190
Thr Leu Lys Gln Thr Tyr Arg Leu Thr Val His Ala Glu Ala Asn Ala
195 200 205
Glu Thr Arg Asp Gln Leu Ile Val Asn Gly Val Tyr Thr Glu Gly Val
210 215 220
Thr Arg Gly Glu Glu Ile Arg Asp Met Lys Lys Met Cys Tyr Ser Arg
225 230 235 240
Phe Arg Asp Gly Trp Ile Arg Ala Thr Ile Gln Pro Lys Ile Val Glu
245 250 255
Glu Leu Arg Arg Ser Lys Glu Ala Leu Asp Arg Ile Ser Ser Glu Trp
260 265 270
Tyr Glu Lys Leu Thr Thr Pro Asp Val Val Glu Ile Cys Lys Ile Val
275 280 285
Ser Ser Ile Gly Arg Gln Met Trp Asn Ser Glu Glu Leu Pro Val Asp
290 295 300
Asp Ala Met Arg Ser Lys Val Phe Gln Glu Glu Leu Arg Leu Leu Phe
305 310 315 320
Arg Val Gly Asn Ser Glu Tyr Asp Thr Ile Gln Ala Ile Gln Ser Gly
325 330 335
Gln Thr Pro Leu Lys Lys Phe Tyr Ala Leu Leu Ala Ile Ala Ala Thr
340 345 350
Asp Ser Tyr Arg Trp Arg Ile Trp Trp Ser Asn Pro Tyr Pro Cys Leu
355 360 365
Arg Gly Thr Ile Ile Ala Cys Glu Met Glu Leu Gly Asp Val Tyr Glu
370 375 380
Thr Leu Arg Ser Thr Phe His Trp Ser Leu Arg Pro Thr Tyr Ala Thr
385 390 395 400
Arg Lys Glu Ile Asp Arg Glu Arg Arg Ala Tyr Pro Tyr Gln Arg Ile
405 410 415
Asn Leu Phe Glu Ser Arg Leu Asp Pro Gly Thr Arg Ile Ile Asn Trp
420 425 430
Leu Val Val Arg Glu Pro Leu Ala Glu Pro Asp Val Lys Ser Gly Arg
435 440 445
Val Cys Met Thr Ser Thr Glu Ser Glu Tyr Ile Leu Lys Val Asp Asp
450 455 460
Thr Lys Tyr Arg Glu Met Ile Gly Gln Ile Ile Glu Arg Gly Trp Glu
465 470 475 480
Lys Glu His Leu Lys Leu Glu Lys Ile Val Gln Asp Glu Gly Asn Val
485 490 495
Phe Gln Met Glu Phe Glu Lys Asp Ala Ser Leu Asp Glu Arg Ser His
500 505 510
Leu Val Met Pro Tyr Tyr Tyr Asp Lys Glu Ile His Cys Pro Met Tyr
515 520 525
His Lys Lys Ile Lys Ile Thr Glu Ala Glu Ile Ala Thr Ser Trp Ser
530 535 540
Asp Asp Pro Trp Leu Asn Arg Val Leu Asn Gly Phe Leu Gly Asp His
545 550 555 560
Val Glu Thr Phe Asn Leu Gly Cys Asn His Ile Phe Asp Met Arg Val
565 570 575
Pro Leu Lys Gly Asn Leu Leu Thr Gln Glu Gln Ala Ile Ser Ser Tyr
580 585 590
His Glu His Ile Arg Ser Pro Glu Ala Gln Ser Glu Asn Gln Cys Ala
595 600 605
Leu Lys Val Tyr Ala Asp Glu Ala Val Ser Lys Leu Phe Thr Thr Leu
610 615 620
Tyr His Arg Leu Ala Tyr His Leu Pro Lys Thr Glu Tyr Ile Glu Arg
625 630 635 640
Glu Glu Glu Val Ser Gln Tyr Glu Gly Leu Val Lys Arg Ile Arg Thr
645 650 655
Trp Asp Gln Phe Ile Ile Phe Val Phe Asp Phe Phe Phe Glu Asn Arg
660 665 670
Lys Gly Leu Arg Thr Thr Glu Glu Ala Thr Gln Leu Ala Lys Ala Ile
675 680 685
Arg Ser Asn Val Gly Ala Thr Arg Trp Thr Met Met Ala Ser Ala Phe
690 695 700
Pro Thr Ser Val Arg Leu Val Lys Gly Ile Leu Arg Ala Lys Thr Trp
705 710 715 720
Gly Glu Cys Tyr Ile Met Asn Phe Leu Pro Leu Leu Ile Cys Gly Gly
725 730 735
Ser Ser Ile Ser Tyr Leu His Arg Glu Trp Ser Tyr His Met Ile Val
740 745 750
Val Phe Glu Asp Gly Ile Lys Val Val Pro Ala Met Thr Gly Arg Ser
755 760 765
Leu Thr Gln Gly Ser Leu Gly Glu Trp Ala Ser Phe Val Cys Phe Phe
770 775 780
Met Gly Ser Asn His Glu Lys Ile Lys Leu Glu Glu Asp Glu Leu Glu
785 790 795 800
Val Leu Arg Lys Cys Val Glu Phe Tyr Glu Gly Val Lys Met Val Asp
805 810 815
Ile Ile Lys Asp Thr Pro Val Arg Met Thr Lys Tyr Ile Ala Cys Asp
820 825 830
Leu Ala Phe Gly Ser Gly Cys Gly Gly Thr Ser Glu Ser Leu Ser Phe
835 840 845
Ile Leu Pro Val Thr His Pro Asn Arg Ser Leu Ile Ile Phe Val Val
850 855 860
Ala Asp Asp Lys Ile Ser Pro Ala Gln His Ala Ala Arg Ala Lys Arg
865 870 875 880
Arg Phe Arg Gly Thr Arg Glu Tyr Val Asn Asp Val Ile Val Leu Gln
885 890 895
Met Lys Arg Pro Gly Thr Val Gly Asn Val Trp Ser Glu Lys His Ser
900 905 910
Lys Val Arg Ile Cys Arg Arg Asn Phe Leu Lys Tyr Asp His Lys Val
915 920 925
Val Leu Thr Lys Phe Ala Gly Leu Val Tyr Gly Asn Tyr Glu Leu Ile
930 935 940
Thr Lys Leu Ala Asn Leu
945 950
<210> 162
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<212> PRT
<213> 人工序列
<220>
<223> BTV12 CAE53027.1的VP5
<400> 162
Met Met Gly Lys Ile Ile Lys Ser Leu Gly Lys Phe Gly Lys Lys Val
1 5 10 15
Gly Asn Ala Leu Thr Ser Lys Thr Ala Gln Lys Ile Tyr Arg Thr Ile
20 25 30
Gly Ser Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala
35 40 45
Ile Asp Gly Leu Ile Gln Gly Ser Val His Ser Ile Met Thr Gly Asp
50 55 60
Ser Tyr Gly Glu Ser Val Lys Gln Ala Val Ile Leu Asn Val Leu Gly
65 70 75 80
Gly Gly Asp Thr Ile Ala Asp Pro Leu Ser Pro Gly Glu Asn Glu Ile
85 90 95
Tyr Arg Lys Leu Lys Gln Leu Glu Asp Glu Gln Lys Gly Glu Ile Val
100 105 110
Arg Thr Lys His Asn Thr Glu Ile Leu Lys Lys Phe Gly Asn Asp Leu
115 120 125
Asp Glu Val Tyr Lys Phe Ala Thr Gln Glu His Lys Val Gly Glu His
130 135 140
Glu Glu Asp Gln Ile Lys Met Leu Glu Lys Ala Leu Lys Ser Tyr Ile
145 150 155 160
Gly Val Phe Lys Ala Glu Asn Asp Ser Ile Asp Lys Leu Lys Lys Ala
165 170 175
Leu Tyr Val Glu Ser Thr Glu Arg Thr Glu Glu Glu Thr Lys Met Ile
180 185 190
Ser Glu Tyr Arg Asp Lys Ile Asp Ala Leu Arg Ser Ala Ile Glu Val
195 200 205
Glu Lys Glu Gly Leu Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met
210 215 220
Ser Ala Glu Val Leu Glu Ala Ala Ala Glu Glu Val Pro Leu Phe Gly
225 230 235 240
Ala Gly Val Ala Thr Ser Ile Ala Thr Ala Arg Ala Ile Glu Gly Ala
245 250 255
Tyr Lys Ile Lys Ala Val Ile Ser Ala Leu Thr Gly Ile Asp Leu Thr
260 265 270
His Leu Asn Val Pro Lys Val Gln Pro Lys Thr Leu Glu Ala Ile Leu
275 280 285
Asp Val Pro Lys Gly Val Asp Ile Pro Asp Leu Arg Leu Thr Glu Gly
290 295 300
Leu Ile Ala Lys Met Asp Lys Ile Ser Glu Asn Ile Ser Glu Val Asp
305 310 315 320
His Ile Gln Asn Val Ile Val Pro Lys Leu Lys Lys Ala Val Glu Glu
325 330 335
Asp Lys Glu Asn Ile Thr Asp Trp Ser Glu Lys Arg Ile His Pro Lys
340 345 350
Thr Ala Gln Arg Phe Lys Ile Pro Gln Thr Gln Lys Pro Ser Met His
355 360 365
Ile Tyr Ala Ala Pro Trp Asp Ser Asp Glu Val Phe Ile Phe His Val
370 375 380
Ile Ser Pro His His Lys Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu
385 390 395 400
Glu Ile Glu Tyr Val Phe Tyr Glu Asp Leu Thr Gln His Trp His Ala
405 410 415
Leu Gly Thr Ala Gln Ser Thr Tyr Gly Arg Thr Phe Arg Glu Ala Tyr
420 425 430
Lys Glu Phe Phe Leu Leu Ala Ser Asn Val Ala Gly Gln Thr Ala Ile
435 440 445
His Gln Arg Arg Leu Leu Arg Ser Arg Ser Asn His Pro Ile Tyr Leu
450 455 460
Gly Met His Asp Tyr Glu Val Ser Tyr Ala Gln Met Lys Met Asn Ala
465 470 475 480
Met Ala Leu Val Ser Asn Glu Asp Leu Gln Met His Ile Leu Arg Gly
485 490 495
Pro Ile His Phe Gln Arg Arg Thr Ile Val Ala Ala Leu Lys Tyr Gly
500 505 510
Ile Lys Ile Leu Thr Gln Pro Asp Asp Val Ser Ile Met Leu Arg Asn
515 520 525
Ala
<210> 163
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<220>
<223> BTV13 CAE51100.1的VP2
<400> 163
Met Glu Glu Leu Val Ile Pro Val Ile Thr Glu Arg Phe Asp Lys Arg
1 5 10 15
Leu Val Gly Arg Tyr Asp Tyr Val Ile Glu Leu Ala Arg Pro Glu Gly
20 25 30
Asp Glu Trp Ser Gly His Asp Val Thr His Ile Pro Asp Lys Arg Met
35 40 45
Phe Asp Ile Lys Val Gln Pro Ile Arg Asp Ala Ile Asp Tyr Lys Pro
50 55 60
Val Glu Asn Asp Gly Glu Val Leu Pro Arg Ile Leu Asp Met Ser Ile
65 70 75 80
Ala Cys Tyr Asp Met Arg Lys Arg Met Met Lys Arg Asp Gly Val Asp
85 90 95
Phe Val Ser Asn Thr Lys Trp Leu Glu Trp Met Ile Gln Asp Ser Met
100 105 110
Asp Val Gln Pro Leu Arg Val Asp Met Lys Glu Asp His Ser Thr Val
115 120 125
Gln Tyr Asp Met Phe Ser Ala Lys Leu His Val Asp Ser Arg Lys Ala
130 135 140
Asp Thr Thr Ser Tyr Asn Ile Leu Ala Leu Glu Val Lys Lys Gly Ala
145 150 155 160
Gln Cys His His Val His Ala Asn Ile Trp Asn His Met Ile Arg Asn
165 170 175
His Leu Phe His Ala Val Gln Glu Ser Cys Tyr Ile Phe Lys Pro Thr
180 185 190
Tyr Lys Leu Ile Val Asn Ser Glu Arg Arg Thr Pro Asp Glu Asp Phe
195 200 205
Gln Ile Gly Asn Pro Gln Phe Pro Thr Leu Arg Arg Asn Gln Gln Ile
210 215 220
Phe Leu Gly Asp Asp Ala Tyr Lys Lys Thr Ala Arg Gly Leu Val Gln
225 230 235 240
Val Leu Val Asn Gly Val Val Pro Asp Ile Ile Arg Asn Glu Ile Ala
245 250 255
Ala Leu Asp Ala Ile Arg Asp Arg Trp Ile Gln Gly Asn Tyr Glu Arg
260 265 270
Thr His Ile Lys Ser Leu Glu Leu Cys Asn Leu Leu Ser Ala Ile Gly
275 280 285
Arg Lys Met Val Asn Leu Glu Glu Glu Pro Lys Asp Glu Arg Asn Leu
290 295 300
Ser Leu Arg Phe Gln His Lys Leu Asp Asp Lys Phe Ala Lys Asn Asp
305 310 315 320
Gln Glu Arg Asn Val Ile Phe Ala Gln Lys Ser Gln Arg Asn Asp Gln
325 330 335
Asp Arg Phe Tyr Val Leu Met Val Ile Ala Ala Ser Asp Thr Asn Asn
340 345 350
Ser Arg Val Trp Trp Ser Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu
355 360 365
Ile Ala Ala Glu Cys Lys Leu Gly Asp Val Tyr Tyr Lys Leu Arg Ser
370 375 380
Trp Tyr Glu Trp Ser Val Arg Glu Gly Tyr Lys Pro Arg Asp Leu Asp
385 390 395 400
Arg Gln Tyr Glu Lys Tyr Ile Val Gly Arg Val Asn Leu Phe Asp Leu
405 410 415
Glu Ala Glu Pro Gly Thr Lys Val Leu His Trp Glu Tyr Glu Leu Ile
420 425 430
Ser Lys Leu Tyr Ile Val Ser Asn His Glu Gly Asn Gln Cys Asp Leu
435 440 445
Tyr Pro Asp Glu Gly Glu Ile Val Ala Lys Phe Asp Asp Ala Arg Tyr
450 455 460
Ser Asp Met Ile Gln Thr Ile Ile Asn Glu Gly Trp Lys Gln Gly Asp
465 470 475 480
Phe Lys Met Phe Lys Val Leu Lys Asp Glu Gly Asn Pro Leu Leu Tyr
485 490 495
Asp Leu Glu Lys Asp Ile Lys Leu Asp Arg Val Ser Arg Val Val Phe
500 505 510
Pro Pro Tyr Phe Asp Gln Trp Thr Tyr Val Pro Met Phe Asn Ala Arg
515 520 525
Ile Lys Pro Cys Glu Val Glu Leu Ala Glu Arg Lys Asn Thr Asp Pro
530 535 540
Tyr Val Lys Arg Thr Leu Lys Pro Leu Lys Ala Asp Cys Ile Glu Leu
545 550 555 560
Met Arg Tyr His Met Ser Gln Tyr Met Asp Leu Arg Val Ser Leu Gln
565 570 575
Gly Thr Ser Leu Ser Ile Lys Gln Val Pro Ser Ser Ile His Gln Ser
580 585 590
Leu Ala Gln Asp Ala Ser Tyr Ala Gly Ile Leu Ser Arg Arg Arg Glu
595 600 605
Asn Leu Asp Tyr Lys Ser Gln Cys Pro Ile Val Thr Asn Leu Phe Leu
610 615 620
Leu Glu Lys Phe Phe Leu Leu Ile Phe Thr Thr Met Glu Lys His Tyr
625 630 635 640
Trp Glu Met Asp Asp Asp Glu Thr Gln Tyr Glu His Pro Lys Ile Asp
645 650 655
Pro Ser Lys Phe Glu Val Glu Gly Thr Leu His Asp Val Ser Gln Val
660 665 670
Met Val His Leu Leu Asp Arg Phe Phe Glu Lys Arg Arg Phe Leu Arg
675 680 685
Thr Val Asp Glu Ser Arg Trp Ile Leu His Leu Ile Arg Ser Thr Ser
690 695 700
Gly Ser Lys Arg Leu Glu Val Leu Ser Arg Phe Phe Pro Ala Phe Ser
705 710 715 720
Asp Gly Leu Arg Ile Arg Glu Phe Lys Lys Val Arg Asp Ile Met Leu
725 730 735
Leu Asn Phe Leu Pro Phe Leu Phe Leu Thr Gly Asp Asn Met Ala Tyr
740 745 750
Glu His Arg Gln Trp Ala Val Pro Val Ile Phe Tyr Ala Asp Lys Ile
755 760 765
Arg Ile Val Pro Ala Glu Val Gly Ala Tyr Tyr Asn Arg Phe Gly Leu
770 775 780
Thr Cys Ile Leu Glu Leu Met Met Phe Phe Pro Ser Tyr Asp Thr Arg
785 790 795 800
Asn Glu Asp Leu Gly Glu Asp Val Arg Ala Cys Ile Gly Pro Ile Ile
805 810 815
Asn Tyr Tyr Leu Asp Thr Thr Ile Ser Asn Gly Gly Ile Gln Thr Ser
820 825 830
Ile Val Ser Thr Lys Ala Leu Leu Tyr Glu Thr Tyr Leu Ser Ser Ile
835 840 845
Cys Gly Gly Phe Ser Glu Ala Ile Leu Trp Tyr Leu Pro Val Thr His
850 855 860
Pro Ser Lys Cys Leu Ile Ala Leu Glu Val Ser Asp Ala Leu Thr Ser
865 870 875 880
Pro Glu Leu Arg Ile Asp Arg Ile Arg Arg Arg Phe Pro Leu Ser Ser
885 890 895
Asn His Leu Lys Gly Ile Val Gln Val Ser Val Arg Pro Asp Arg Thr
900 905 910
Phe Ser Val Ile Thr Gln Gly Ile Val Lys His Arg Val Cys Lys Lys
915 920 925
Thr Leu Leu Arg His Arg Cys Asp Val Ile Leu Ile Gln Thr Pro Gly
930 935 940
Tyr Val Phe Gly Asn Asp Glu Leu Leu Thr Lys Leu Leu Asn Ile
945 950 955
<210> 164
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV13 CAE53029.1的VP5
<400> 164
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Asn Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ala
65 70 75 80
Gly Asp Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Met Lys Ile Lys Glu Ile Glu Glu Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Ile Lys Tyr Gly Lys Glu Ile Arg Glu Lys Phe Gly Glu Gln Leu Glu
115 120 125
Glu Ile Tyr Gln Phe Met Asn Gly Glu Val Lys Gly Glu Glu Glu Gln
130 135 140
Glu Glu Gln Tyr Lys Val Leu Cys Lys Ala Val Asp Ser Tyr Glu Asn
145 150 155 160
Leu Leu Val Thr Glu Asn Glu Gln Met Arg Thr Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Thr Glu Arg Thr Glu Asn Glu Ser Lys Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Ala Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Val Gly Ser
225 230 235 240
Gly Met Ala Thr Ala Ile Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Thr Met Val Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Lys Glu Ile Pro Asp Lys Glu Leu Ala Val Ser Val Leu
290 295 300
Ala Lys Asn Asp Ala Ile Val Ala Asn Thr Lys Glu Ile Lys His Ile
305 310 315 320
Lys Glu Glu Ile Leu Pro Lys Phe Lys Lys Ile Met Glu Glu Glu Lys
325 330 335
Glu Leu Glu Gly Ile Asp Asp Lys Lys Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Val Pro Arg Ser Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
Phe His Ala Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Ala Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Glu Ala Lys Gly Arg Thr Leu Asn Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ser Ile Gly Ser Thr Phe Thr Ser Pro Met His Ala Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Thr Leu Lys Thr Asn Ala Gln Lys Leu
465 470 475 480
Val Tyr Asp Glu Asp Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Val
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Lys Asn Ala
515 520 525
<210> 165
<211> 955
<212> PRT
<213> 人工序列
<220>
<223> BTV14 CAE51101.1的VP2
<400> 165
Met Asp Glu Leu Ala Ile Pro Ile Ile Asn Gly His Phe Pro Lys Glu
1 5 10 15
Thr Leu Ile Glu Tyr Asp Cys Ile Ile Glu Leu Asn Pro Pro Val Glu
20 25 30
Asp Asp Ala Ala Asp Val Val Gln Ile Pro Thr Arg Asp Met Met Asp
35 40 45
Ile Pro Asn Val Pro Ile Arg Asp Ala Leu Lys His Leu Pro Gln Arg
50 55 60
Asn Asp Gly His Val Leu Thr Arg Val Leu Asp Ile Ser Leu Ser Gly
65 70 75 80
Tyr Asp Arg Arg Lys Tyr His Lys Thr Asn Ser Gly Gly Glu Phe Tyr
85 90 95
Ser Pro Asn Glu Trp Leu Asn Trp Met Ile Asn Asp Ser Met Asp Val
100 105 110
Gln Pro Leu Lys Val Ser Leu Gly Leu Pro Pro Thr Ile Arg His Glu
115 120 125
Leu Phe Ser Ser Ser Val Phe Ile Ser Ala Lys Lys Ala Asp Thr Thr
130 135 140
Ser Tyr His Ile Glu Pro Ile Cys Glu Lys Asp Lys Ala Cys Asp His
145 150 155 160
Thr Arg Val Ser Met Trp Asn Asn Leu Thr Arg His Glu Met Met His
165 170 175
Cys Ala Gln Glu Ser Ala Tyr Val Leu Lys Pro Thr Tyr Asp Ile Thr
180 185 190
Val Tyr Ser Glu Arg Ala Thr Thr Asp Glu Ala Phe Gln Gln Val Gly
195 200 205
Gln Lys Phe Val Thr Ile Thr Lys Asn His Arg Val Val Leu Gly Asp
210 215 220
Asp Ala Tyr Lys Lys Thr Leu Gly Gly Leu Thr Arg Leu Arg Val Gln
225 230 235 240
Gly Lys Ile Pro Ser Leu Ile Gln Asn Asp Ile Gln Gln Leu Asn Gln
245 250 255
Ile Arg Asp Thr Trp Ile Arg Asp Ser Tyr Asn Pro Arg Asn Ile Arg
260 265 270
Ser Leu Glu Leu Cys Lys Leu Leu Ser Gly Ile Gly Arg Lys Met Val
275 280 285
Cys Ile Glu Glu Glu Pro Lys Asn Glu Ser Asp Leu Ser Val Lys Phe
290 295 300
Gln Phe Lys Leu Asp Glu Lys Phe Arg Leu Asp Asp Ser Glu Arg Gly
305 310 315 320
Val Ile Phe Thr Asn Lys Gly Gln Arg Asn Asp Gln Asp Arg Phe Tyr
325 330 335
Val Leu Ile Met Ile Ala Ala Ser Asp Thr Tyr Asn Ser Arg Ile Trp
340 345 350
Trp Thr Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu Ile Ala Ala Glu
355 360 365
Thr Gln Leu Gly Asp Val Tyr Trp Thr Leu Arg His Phe Tyr Glu Trp
370 375 380
Ser Val Arg Pro Ala Tyr Ser Pro Arg Leu Arg Glu Arg Glu Asp Asp
385 390 395 400
Lys Tyr Ile Tyr Gly Arg Val Asn Leu Phe Asp Leu Asp Ala Ser Pro
405 410 415
Gly Thr Lys Ile Ile His Trp Thr Tyr Asp Leu Ile Thr Arg Glu Ser
420 425 430
Lys Ile Thr Tyr His Asn Gly Asn Pro Cys Asp Leu Asn Pro Asp Val
435 440 445
Asp Gly Ile Ile Thr Lys Phe Asn Lys Thr Ala Tyr Thr Asp Met Ile
450 455 460
Gly Glu Leu Leu Asn Gly Gly Trp Asn Thr Arg Glu Phe Lys Met Tyr
465 470 475 480
Lys Ile Leu Glu Ser Val Gly Asn Val Leu Thr Ile Asp Phe Glu Lys
485 490 495
Asp Ala Lys Leu Asn Ser Arg Ser Glu Phe Ile Leu Pro Asp Tyr Tyr
500 505 510
Asp Lys Trp Ile Tyr Ala Pro Met Phe Asn Ala Lys Leu Lys Ile Thr
515 520 525
Glu Val Glu Ile Ala Gln Ala Lys Ser Asp Asp Pro Asp Val Val Arg
530 535 540
Thr Leu Ala Pro Ile Gly Ser Asp Pro Ile Thr Leu Gln Arg Leu Cys
545 550 555 560
Leu Gly Asn Tyr Tyr Asp Ile Arg Pro Ala Met His Gly Arg Ala Leu
565 570 575
Met Lys Lys Gln Glu Gln Thr Ser Tyr His Lys Thr Leu Ala Lys Thr
580 585 590
Lys Asp Tyr Ser Asp Val Leu Asn Arg Arg Gly Tyr Arg Lys Val Glu
595 600 605
Lys Pro Cys Pro Met Val Thr Cys Ser Tyr Val Leu Glu Lys Thr Ala
610 615 620
Leu Phe Trp Ile Asp Val Met Glu Lys His Val Gly Asp Ile Glu Asp
625 630 635 640
Ser Thr Asp Glu Phe Asp Phe Pro Arg Val Asp Val Thr Leu Pro Val
645 650 655
Glu Thr His Lys Val Val Asp Val Ala Gln Leu Ser Val Leu Leu Ile
660 665 670
Asp Phe Leu Tyr Glu Lys Arg Arg Thr Val Arg Asp Ile Asp Ser Ala
675 680 685
Arg Trp Thr Ile Glu Leu Ile Arg Arg Val Arg Gly Lys Asp Arg Leu
690 695 700
Glu Ala Ile Arg Arg Glu Phe Pro Asn Phe Gly Glu Ala Val Thr Ile
705 710 715 720
Leu Met Asn Pro Thr Arg Val Lys Asp Val Met Val Ile Asn Phe Leu
725 730 735
Pro Phe Leu Ile Leu Leu Gly Asp Asn Ile Ser Tyr Glu His Arg Gln
740 745 750
Trp Ser Ile Pro Ile Ile Leu His Ala Asp Ser Met Trp Phe Leu Pro
755 760 765
Val Glu Val Gly Ala Phe Tyr Asn Arg Phe Gly Val Ile Ser Phe Leu
770 775 780
Glu Tyr Met Met Phe Phe Pro Ser Ala Gln Ser Arg Glu Ser Glu Leu
785 790 795 800
Asp Glu Ala Lys Ile Lys Val Ser Lys Leu Ile Leu Glu Phe Tyr Leu
805 810 815
Asp Thr Thr Ile Phe Ser Gly Gly Val Gln Arg Ser Val Val Ser Thr
820 825 830
Lys Gln Ile Leu Tyr Glu Thr Tyr Leu Ser Ser Leu Cys Gly Gly Tyr
835 840 845
Ser Glu Gly Leu Val Trp Tyr Leu Pro Ile Thr His Pro Thr Lys Cys
850 855 860
Val Val Ala Ile Glu Phe Ser Asp Asp Arg Val Asp Ala Ala His Arg
865 870 875 880
Cys Asn Arg Leu Arg Ala Arg Phe Pro Leu Ser Ala Val His Leu Lys
885 890 895
Gly Ile Val Ile Leu Ser Ile Ala Lys Asp Arg Ala Val Lys Ala Tyr
900 905 910
Thr Glu Gly Ile Val Thr His Arg Leu Cys Lys Lys Asn Val Leu Gly
915 920 925
Phe Thr Cys Gln Ile Leu Leu Leu Lys Phe Ser Gly His Val Phe Gly
930 935 940
Asn Asp Glu Met Leu Thr Lys Leu Leu Asn Val
945 950 955
<210> 166
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<212> PRT
<213> 人工序列
<220>
<223> BTV14 CAE53030.1的VP5
<400> 166
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ala
65 70 75 80
Gly Asp Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Met Lys Ile Lys Glu Leu Glu Glu Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys His Gly Lys Glu Ile Thr Lys Lys Phe Gly Glu Glu Leu Glu
115 120 125
Glu Ile Tyr Gln Phe Met Asn Gly Glu Val Lys Asp Glu Asp Glu Gln
130 135 140
Lys Lys Gln Tyr Glu Met Leu Cys Lys Ala Val Asp Ser Tyr Glu Lys
145 150 155 160
Leu Leu Val Ala Glu Asn Asp Glu Met Arg Thr Leu Ala Arg Ala Leu
165 170 175
Arg Arg Glu Thr Thr Glu Arg Thr Glu Val Glu Ser Lys Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Ala Ala Ile Glu Val Gln
195 200 205
Arg Asp Gly Met Gln Glu Glu Gly Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Val Gly Ser
225 230 235 240
Gly Met Ala Thr Ala Ile Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Thr Met Phe Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Arg Asp Ile Pro Asp Lys Glu Leu Ala Val Ser Ile Leu
290 295 300
Ala Lys Asn Asn Ala Ile Val Ala Asn Thr Arg Glu Val Gln His Ile
305 310 315 320
Lys Glu Glu Ile Leu Pro Lys Phe Lys Lys Ile Met Asp Glu Glu Lys
325 330 335
Glu Leu Glu Gly Ile Asp Asp Lys Arg Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Val Pro Arg Ser Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
Phe His Met Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Ala Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Glu Ala Lys Gly Arg Thr Leu Asn Glu Ala Tyr Lys Glu Phe
420 425 430
Leu Asn Leu Ser Ile Gly Ser Thr Tyr Thr Ser Pro Met His Ala Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Thr Leu Lys Ser Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Val
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Lys Asn Ala
515 520 525
<210> 167
<211> 952
<212> PRT
<213> 人工序列
<220>
<223> BTV14 CAE51102.1的VP2
<400> 167
Met Glu Asp Phe Pro Val Ser Val Ile Glu Arg Asn Ser Thr Ile Ala
1 5 10 15
Pro Ala Ile Ile Lys Arg Tyr Pro Ile Ile Val Asp Val Arg Asn Met
20 25 30
Val Ala Leu Ser Gly Gly Lys Asn Asp Val Thr Asn Ile Pro Arg Val
35 40 45
Arg Asn Ile Gly Val Arg Gln Glu Gly Ile Arg Val Gly Leu Lys Tyr
50 55 60
Arg Pro Asp Thr Glu Thr Asn Val Ile Phe Pro Lys Asn Leu Asp Val
65 70 75 80
Leu Ile Arg Ala Tyr Asp His Lys His Arg Leu Thr Arg Asp Lys Arg
85 90 95
Pro Thr Asp His Asp Val Asp Ser Ile Ser Asp Gly Asp Val Thr Val
100 105 110
Trp Val Thr Asn Arg Thr Asn Glu Phe Tyr Asp Ile Gln Pro Ile Tyr
115 120 125
His Thr Leu Glu Asp Glu Val Cys Lys Val Lys Phe Thr Ser Leu Leu
130 135 140
Gly Ser Val Ser Thr Asp Ser Asn Tyr Ala Glu Ser Met Ser Tyr His
145 150 155 160
Tyr Ile Gly Val Glu Arg Asp Lys Cys Asp His Ser Lys Trp His Arg
165 170 175
Phe Phe Pro Met Leu Leu Ser Asp Ser Ile Asn Leu Gly Lys Glu Val
180 185 190
Gly Tyr Ile Leu Lys Lys Thr Tyr Lys Ile Lys Ala Ile Gly Glu Ala
195 200 205
Arg Glu Gly Gln Arg Gln Glu Leu Arg Val Asp Val Pro Tyr Thr Pro
210 215 220
Gly Ile Gly Arg Asn Glu Arg Ile Ser Asp Met Lys Leu Pro Val Tyr
225 230 235 240
Lys Arg Phe Leu Glu Gly Tyr Ile Arg Cys Gln Ile Glu Pro Asn Val
245 250 255
Pro Glu Lys Leu Thr Glu Leu Lys Asn Gln Leu Asp Ala Ile Cys Val
260 265 270
Ala Trp Tyr Gly Gly Gln Val Pro Ile Val Ala Asp Glu Ile Cys Lys
275 280 285
Ile Thr Ser Arg Ile Gly Arg Leu Met Trp Asn Thr Glu Glu Glu Pro
290 295 300
Val Asp Glu Thr Met Arg Ser Arg Ala Phe Gln Glu Ser Ile Arg Leu
305 310 315 320
Met Phe Arg Thr Glu Asn Ser Glu Tyr Asn Asn Ile Gln Ala Ile Gly
325 330 335
Ser Gly Arg Thr Gln Arg Gln Lys Phe Tyr Ala Pro Leu Ile Ile Ala
340 345 350
Ala Thr Asp Ser Phe Arg Trp Arg Ile Trp Trp Asn Asn Pro Tyr Pro
355 360 365
Cys Leu Arg Gly Cys Leu Ile Ala Cys Glu Met Glu Leu Gly Asp Val
370 375 380
Tyr Lys Ser Leu Lys Ser Ile Tyr Lys Trp Thr Leu Arg Ser Ser Tyr
385 390 395 400
Ser Ser Gln Arg Glu Ile Asp Arg Gln Leu Asn Val Phe Pro Tyr Gln
405 410 415
Lys Ile Asn Leu Phe Asp Phe Ser Gly Thr Pro Gly Thr Glu Ile Ile
420 425 430
His Trp Arg Ile Val His Ile Pro Val Pro Asp Glu Ile Asp Tyr Glu
435 440 445
His Gly Tyr Leu Cys Pro Glu Ser Gly Asp Tyr Asp Ile Val Met Ala
450 455 460
Val Asp Asp Asp Leu Tyr Ser Ala Phe Lys His Arg Val Ile Asp Arg
465 470 475 480
Gly Trp Glu His Gln Ala Met Lys Leu Asp Glu Leu Val Pro Gln Glu
485 490 495
Asn Asn Ile Phe Lys Met Glu Phe Glu Lys Asp Ala Tyr Leu Asp Asn
500 505 510
Arg Asn His Leu Val Leu Pro Pro Tyr Tyr Asn Lys Val Val Tyr Cys
515 520 525
Pro Leu Phe His Ala Thr Ala Lys Ile Thr Pro Thr Glu Ile Ser His
530 535 540
Arg Gln Ser Asp Asp Pro Trp Cys Glu Arg Thr Leu Tyr Gly Phe Arg
545 550 555 560
Gly Asp His Val Ile Thr His Tyr Thr Gly Leu Asn His Ile Val Asn
565 570 575
Arg Arg Lys Pro Leu Met Gly Gln Thr Leu Ser Val Ala Gln Ser Met
580 585 590
Gly Asp Tyr Thr Lys Tyr Ile Leu Gly Asp Asp Gly Ser Ala Asn Ser
595 600 605
Cys Thr Gly Thr Ala Tyr Ser Ser Arg Leu Pro Thr Met Leu Phe Gln
610 615 620
Asp Ile Phe Asp Ala Ile Ile Arg His Leu Pro Lys Val Ile Leu Lys
625 630 635 640
Asp Gln Ala Asp Tyr Phe Ser Glu Ser Arg Lys Arg Val Ser Asp Ile
645 650 655
Ile Asp Trp Asp Thr Phe Ile Val Tyr Ser Ile Tyr Ala Cys Phe Glu
660 665 670
Arg Lys Asn Gln Val Leu Arg Glu Glu Asp Ala Thr Gln Ile Val Arg
675 680 685
Gly Ile Ser Thr Ala Gly Lys Glu Lys Arg Leu Lys Leu Ile Glu Arg
690 695 700
Ser Phe Pro Ile Tyr Tyr Arg Glu Leu Met Lys Ile Lys Asn Ala Lys
705 710 715 720
Thr Trp Lys Asp Ala Tyr Ala Phe Asn Phe Leu Pro Leu Leu Leu Cys
725 730 735
Ala Gly Thr Asn Val Gln Tyr Val His Arg Gln Trp Ser Tyr Pro Val
740 745 750
Leu Val Ser Met Glu Lys Gly Pro Arg Ile Ile Pro Ala Met Val Gly
755 760 765
Arg Asp Leu Ala Asp Gly Asn Leu Gly Asp Trp His Ala Tyr Ile Lys
770 775 780
Tyr Tyr Met Gly Gln His Met Glu Asp Gln Glu Val Glu Glu Asp Ser
785 790 795 800
Leu Glu Ile Leu Lys Ile Ser Leu Glu His Tyr Met Ser His Ser Tyr
805 810 815
Ile Ser Asn Val Lys Asp Val Ala Val Arg Met Thr Lys Glu Ile Val
820 825 830
Leu Asp Leu Tyr Phe Ala Ser Gly Cys Asn Gly Val Ser Glu Met Met
835 840 845
Thr Phe Leu Leu Pro Ile Ile His Pro Arg Lys Gly Leu Val Met Phe
850 855 860
Cys Ile Ala Asp Asp Gln Val Gln Pro Gln Val Gln Cys Glu Thr Ala
865 870 875 880
Leu Arg Arg Phe Pro Tyr Thr Arg Glu Tyr Ile His Asp Val Ile Val
885 890 895
Ile Gln Val Gly Arg Pro Gly Glu Leu Lys Gln Val Trp Lys Arg Gly
900 905 910
Ser Thr Lys Val Lys Val Cys Arg Arg Asn Phe Leu Arg Tyr Asp His
915 920 925
Lys Val Ile Leu Ser Arg Leu Cys Gly Leu Val Tyr Gly Asn Tyr Glu
930 935 940
Leu Met Thr Lys Leu Thr Asn Ile
945 950
<210> 168
<211> 527
<212> PRT
<213> 人工序列
<220>
<223> BTV15 CAE53032.1的VP5
<400> 168
Met Gly Lys Ile Ile Lys Ser Leu Gly Lys Phe Gly Lys Lys Ile Ser
1 5 10 15
Ser Ala Leu Gly Ser Ser Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Val Glu Ser Asp Leu Gly Ser Ala Thr Ile
35 40 45
Asp Gly Ile Ile Gln Gly Ser Val His Ser Ala Leu Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Ile Leu Asn Val Val Gly Gly
65 70 75 80
Gly Asp Ser Ile Ala Asp Pro Leu Ser Pro Gly Glu Asn Asp Met Val
85 90 95
His Arg Leu Lys Arg Leu Glu Asp Glu Gln Lys Gly Glu Ile Ile Arg
100 105 110
Thr Lys His Asn Ala Gln Val Ile Ala Lys Phe Gly Arg Asp Leu Glu
115 120 125
Asp Val Tyr Lys Phe Ala Ser Arg Glu His Lys Val Gly Glu Glu Glu
130 135 140
Glu Asp Gln Ile Gln Met Leu Glu Lys Ala Leu Arg Ala Tyr Gly Asn
145 150 155 160
Val Val Lys Val Glu Gly Glu Ser Val Gln Lys Leu Ala Lys Ala Leu
165 170 175
Arg Val Asp Glu Glu Glu Arg Thr Asp Glu Glu Thr Arg Met Ile Gly
180 185 190
Glu Tyr Arg Asp Lys Ile Asp Ala Leu Ser Lys Ala Ile Glu Ile Glu
195 200 205
Arg Glu Gly Leu Gln Asp Glu Ala Ile Gln Glu Ile Ala Gly Met Ser
210 215 220
Ala Glu Val Leu Glu Ala Ala Ala Glu Glu Val Pro Ile Phe Gly Ala
225 230 235 240
Gly Val Ala Thr Ser Ile Ala Thr Ala Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Ala Val Ile Thr Ala Leu Thr Gly Ile Asp Leu Ser His
260 265 270
Met Thr Thr Pro Lys Ile Gln Pro Lys Thr Leu Glu Ala Ile Leu Asp
275 280 285
Ala Pro Gly Gly Asn Val Thr Asp Leu Met Leu Thr Arg Gly Leu Asp
290 295 300
Ser Lys Ile Asp Lys Ile Asn Glu Asn Leu Ala Glu Val Glu His Met
305 310 315 320
Gln Thr Ser Ile Leu Pro Arg Ile Lys Lys Ala Ile Glu Glu Asp Arg
325 330 335
Asp Glu Ile Thr Asn Trp Ser Pro Lys Arg Ile His Pro Lys Ser Val
340 345 350
Gln Arg Phe Arg Val Pro Arg Met Gln Thr Pro Ser Ile His Ile Tyr
355 360 365
Ala Ala Pro Trp Asp Ser Asp Ser Val Phe Ile Phe His Val Ile Ser
370 375 380
Pro His His Leu Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Glu Ile
385 390 395 400
Glu Tyr Val His Tyr Glu Asp Leu Ala Gln His Trp His Ser Leu Gly
405 410 415
Ala Ala Gln Glu Val Thr Gly Arg Thr Phe Arg Glu Ala Tyr Arg Glu
420 425 430
Phe Phe Asn Leu Ala Ser Arg Ser Thr Met Ala Ser Asp Ile His Lys
435 440 445
Lys Arg Leu Gln Arg Ser Arg Met Ser His Pro Ile Tyr Leu Gly Val
450 455 460
His Asn Tyr Glu Leu Ser Tyr Ile Ala Ile Lys Ser Asn Ala Met Gln
465 470 475 480
Leu Val Thr Asp Glu Asp Leu Gln Lys His Val Leu Arg Gly Pro Leu
485 490 495
His Phe Gln Arg Arg Val Ile Met Ala Ala Leu Lys Tyr Gly Val Lys
500 505 510
Val Met Ser Arg Ala Asp Asp Ile Ala Met Met Leu Arg Asp Ala
515 520 525
<210> 169
<211> 959
<212> PRT
<213> 人工序列
<220>
<223> BTV16 CAE51103.1的VP2
<400> 169
Met Glu Glu Leu Val Ile Pro Val Ile Thr Arg Gln Phe Asp Lys Lys
1 5 10 15
Leu Val Gly Arg Tyr Asp Tyr Val Ile Glu Leu Thr Glu Pro Glu Asp
20 25 30
Gly Glu Trp Ser Gly His Asp Val Thr Glu Ile Pro Asn Arg Arg Met
35 40 45
Phe Asp Ile Lys Gln Gln Asn Ile Arg Glu Ala Ile Glu Tyr Lys Pro
50 55 60
Val Asp Asn Asp Gly Glu Val Leu Pro Arg Ile Leu Asp Met Ser Ile
65 70 75 80
Ala Cys Tyr Asp Met Lys Lys Ser Met Met Lys Lys Asp Gly Val Asp
85 90 95
Phe Val Ser Asn Thr Arg Trp Leu Glu Trp Met Ile Gly Asp Ser Met
100 105 110
Asp Val Gln Pro Leu Arg Val Gln Leu Lys Glu Asp His Ser Thr Ile
115 120 125
Gln Tyr Gly Met Phe Ser Asn Thr Leu His Ile Asp Ser Arg Lys Ala
130 135 140
Asp Thr Thr Ser Tyr His Thr Ile Ala Val Glu Ser Lys Gly Glu Arg
145 150 155 160
Gly Cys Cys His Val His Thr Ala Ile Trp Asn His Met Val Arg Asn
165 170 175
His Leu Phe Asn Ala Val Gln Glu Ala Cys Tyr Val Phe Lys Pro Thr
180 185 190
Tyr Asp Leu Ile Val Ile Gly Glu Lys Gln Asn Arg Glu Asp Glu Phe
195 200 205
Arg Ile Gly Glu His Asn Phe Tyr Thr Ile Thr Arg Asn His His Met
210 215 220
Arg Leu Gly Asp Asn Ala Tyr Asn Gln Phe Met Lys Gly Leu Val Gln
225 230 235 240
Leu Arg Val Ala Gly Val Thr Pro Asn Ala Ile Arg Glu Glu Met Ala
245 250 255
Ala Leu Asp Ala Ile Arg Asp Thr Trp Ile Gly Gly Asn Phe Glu Arg
260 265 270
Thr His Ile Lys Ser Leu Glu Ile Cys Lys Leu Leu Ser Ser Ile Gly
275 280 285
Arg Lys Met Val Asn Met Glu Glu Glu Pro Lys Asp Glu Arg Asp Leu
290 295 300
Ser Val Lys Phe Gln Phe Lys Leu Asp Asp Lys Phe Ser Thr Thr Asp
305 310 315 320
Pro Glu Arg Asn Val Ile Phe Thr His Lys Thr His Arg Thr Asn Gln
325 330 335
Asp Arg Phe Tyr Val Ser Leu Met Ile Ala Ala Ser Asp Thr Asn Asn
340 345 350
Gly Arg Val Trp Trp Ser Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu
355 360 365
Ile Ala Ser Glu Cys Lys Leu Gly Asp Val Tyr His Lys Leu Arg Ala
370 375 380
Trp Tyr Glu Trp Ser Val Arg Pro Glu Tyr Lys Pro Arg Asp Leu Glu
385 390 395 400
Arg Glu Gln Glu Lys Tyr Ile Val Gly Arg Val Asn Leu Phe Asp Leu
405 410 415
Glu Gly Glu Pro Ala Thr Lys Val Phe His Trp Glu Tyr Glu Leu Ile
420 425 430
Asn Lys Val Tyr Gln Ile Thr Asn His Thr Gly Asn His Cys Asp Leu
435 440 445
Tyr Pro Asp Asp Val Glu Ile Thr Ala Lys Phe Asp Glu Glu Lys Tyr
450 455 460
Gly Glu Met Ile Gln Met Ile Ile Asn Glu Gly Trp Lys His Gly Asp
465 470 475 480
Phe Lys Met Phe Lys Ile Leu Lys Glu Glu Gly Asn Pro Leu Leu Tyr
485 490 495
Asp Leu Glu Lys Asp Ile Arg Leu Asp Ser Arg Ser Gln Val Ile Phe
500 505 510
Pro Pro Tyr Phe Asn Lys Trp Thr His Ala Pro Met Phe Asn Ala Lys
515 520 525
Val Lys Pro Cys Glu Val Glu Leu Ala Gln Arg Lys Asn Glu Asp Pro
530 535 540
Tyr Val Lys Arg Thr Val Lys Pro Ile Arg Ala Asp Cys Ile Asp Leu
545 550 555 560
Leu Arg Tyr His Met Ser His Tyr Met Asp Val Arg Val Ser Met Lys
565 570 575
Gly Leu Ser Leu Ala Val Lys Gln Thr Pro Ser Ser Ile His Gln Glu
580 585 590
Trp Ala Lys Asp Pro Leu Tyr Pro Gly Phe Leu Gln Arg Arg Asp Glu
595 600 605
Asn Leu Asp His Lys Ser Val Cys Pro Ile Val Thr Asn Tyr Phe Trp
610 615 620
Leu Glu Arg Phe Tyr Thr Leu Val Leu Thr Ile Met Glu Lys His Tyr
625 630 635 640
Trp Asp Leu Asp Asp Ser Asp Val Thr Tyr Glu Phe Pro Thr Leu Asp
645 650 655
Asp Ser Ala Tyr Lys Ile Glu Gly Thr Leu Tyr Asp Val Ser Gln Val
660 665 670
Val Val His Met Ile Asp Arg Phe Phe Glu Lys Arg Arg Phe Leu Arg
675 680 685
Ser Val Asp Glu Cys Arg Trp Ile Leu His Leu Ile Arg Ser Thr Arg
690 695 700
Ser Arg Asp Arg Leu Ser Val Phe Lys Arg Phe Phe Pro Thr Phe Gly
705 710 715 720
Glu Gly Leu Cys Val Asn Asn Phe Arg Lys Val Lys Asp Ile Met Leu
725 730 735
Leu Asn Phe Leu Pro Phe Phe Phe Leu Ile Gly Asp Asn Met Ala Tyr
740 745 750
Glu His Arg Gln Trp Ala Val Pro Leu Leu Phe Tyr Ala Asp Lys Ile
755 760 765
Arg Val Ile Pro Ala Glu Val Gly Ala Tyr Tyr Asn Arg Phe Gly Leu
770 775 780
Val Ser Ile Leu Glu Leu Leu Thr Phe Phe Pro Ser Tyr Glu Met Arg
785 790 795 800
Asp Asn Lys Val Glu Glu Asp Val His Ala Cys Ala Ser Ala Ile Leu
805 810 815
Asp Phe Tyr Leu Thr Thr Thr Ile Ser Asn Gly Gly Ile Gln Ala Ser
820 825 830
Ile Val Ser Thr Lys Ala Leu Leu Tyr Glu Thr Tyr Leu Ser Ser Val
835 840 845
Cys Gly Gly Phe Ser Glu Ala Ile Leu Trp Tyr Leu Pro Ile Thr His
850 855 860
Pro Val Lys Cys Leu Val Ala Leu Glu Val Ser Asp Ser Leu Val Asp
865 870 875 880
Ser Asn Val Arg Ile Asp Lys Ile Lys Arg Arg Phe Pro Leu Ser His
885 890 895
Arg His Leu Arg Gly Ile Val Gln Ile Ser Val Arg Pro Asp Arg Thr
900 905 910
Phe Gly Val Thr Thr Cys Gly Ile Val Lys His Lys Ile Cys Lys Lys
915 920 925
Thr Leu Leu Lys Gln Arg Cys Asp Val Ile Leu Ile Gln Ile Pro Gly
930 935 940
Tyr Val Phe Gly Asn Asp Glu Leu Leu Thr Lys Leu Leu Asn Ile
945 950 955
<210> 170
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV16 CAE53035.1的VP5
<400> 170
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ala
65 70 75 80
Gly Asp Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Met Lys Ile Lys Glu Ile Glu Glu Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys His Gly Lys Glu Ile Thr Lys Lys Phe Gly Glu Glu Leu Glu
115 120 125
Glu Ile Tyr Gln Phe Met Asn Gly Glu Val Lys Asp Glu Glu Glu Gln
130 135 140
Glu Glu Gln Tyr Lys Val Leu Cys Lys Ala Val Asn Ser Tyr Glu Lys
145 150 155 160
Leu Leu Ile Ala Glu Asn Asp Lys Met His Ile Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Ala Glu Arg Thr Glu Ala Glu Ser Thr Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Ala Ala Ile Glu Ile Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Val Gly Ser
225 230 235 240
Gly Met Ala Thr Ala Ile Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Thr Met Val Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Glu Asp Ile Pro Asp Lys Lys Leu Ala Met Ser Ile Leu
290 295 300
Ala Lys Asn Asn Ala Ile Thr Ala Asn Thr Arg Glu Ile Gln His Ile
305 310 315 320
Lys Glu Glu Ile Leu Pro Lys Phe Lys Lys Val Met Asp Glu Glu Lys
325 330 335
Glu Leu Glu Gly Ile Asp Asp Lys Lys Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Val Pro Arg Ser Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Ile Ser His
370 375 380
Phe His Ala Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Ala Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Glu Ala Lys Gly Arg Thr Leu Asn Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ala Ile Gly Ser Thr Tyr Thr Ser Pro Met His Ala Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Asp Thr Leu Lys Ser Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Val
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Lys Asn Ala
515 520 525
<210> 171
<211> 955
<212> PRT
<213> 人工序列
<220>
<223> BTV17 CAE51104.1的VP2
<400> 171
Met Glu Glu Phe Val Ile Pro Val Tyr Ser Glu Asp Glu Ile Pro Tyr
1 5 10 15
Ala Leu Leu Ser Arg Tyr Pro Leu Ala Ile Gln Thr Asn Val Lys Ile
20 25 30
Glu Asp Val Glu Gly Lys His Asn Val Val Lys Ile Pro Glu Ser Asp
35 40 45
Met Ile Asp Ile Pro Lys Leu Thr Ile Val Glu Ala Met Asn Tyr Lys
50 55 60
Pro Ala Arg Asn Asp Gly Ile Val Val Pro Arg Leu Leu Asp Ile Thr
65 70 75 80
Leu Arg Ala Tyr Asp Asp Arg Lys Ser Thr Lys Ser Ala Arg Gly Ile
85 90 95
Glu Phe Met Thr Asn Ala Arg Trp Met Lys Trp Ala Ile Asp Asp Arg
100 105 110
Met Asp Ile Gln Pro Leu Lys Val Thr Leu Asp His Tyr Cys Ser Val
115 120 125
Asn His Gln Leu Phe Asn Cys Val Val Lys Ala Asn Ala Ala Asn Ala
130 135 140
Asp Thr Ile Tyr Tyr Asp Tyr Phe Pro Leu Glu Asp Tyr Lys Lys Arg
145 150 155 160
Cys Asn His Thr Asn Leu Asp Leu Leu Arg Ser Leu Thr Asn Met Glu
165 170 175
Leu Phe His Ala Leu Gln Gly Ala Ala Tyr Ser Ile Lys Ser Ser Tyr
180 185 190
Glu Leu Val Ala Tyr Ser Glu Arg Gly Ser Leu Glu Glu Thr Tyr Val
195 200 205
Val Gly Gln Pro Lys Trp Ile His Leu Thr Arg Gly Thr Arg Ile Gly
210 215 220
Asn Ser Gly Leu Ser Tyr Glu Arg Phe Ile Ser Ser Met Val Gln Val
225 230 235 240
Ser Val Asn Gly Lys Ile Pro Asp Glu Ile Ala Asn Glu Ile Ala Gln
245 250 255
Leu Asn Arg Ile Arg Ala Glu Trp Ile Thr Ala Thr Tyr Asp Arg Gly
260 265 270
Arg Ile Arg Ala Leu Glu Leu Cys Asn Ile Leu Ser Thr Ile Gly Arg
275 280 285
Lys Met Leu Asn Thr His Glu Glu Pro Lys Asp Glu Met Asp Leu Ser
290 295 300
Thr Arg Phe Gln Phe Lys Leu Asp Glu Lys Phe Asn Arg Ala Asp Ser
305 310 315 320
Glu His Val Asn Ile Phe Gly Val Arg Gly Pro Ala Thr Asp Glu Gly
325 330 335
Arg Phe Tyr Ala Leu Ile Ala Ile Ala Ala Thr Asp Thr Gln Lys Gly
340 345 350
Arg Val Trp Arg Thr Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu Val
355 360 365
Ala Ala Glu Cys Glu Leu Gly Asp Val Tyr Ser Thr Leu Arg Arg Val
370 375 380
Tyr Arg Trp Ser Leu Arg Pro Glu Tyr Gly Gln His Glu Arg Gln Leu
385 390 395 400
Glu Asn Asn Lys Tyr Val Phe Asn Arg Ile Asn Leu Phe Asp Ser Asn
405 410 415
Leu Ala Val Gly Asp Gln Ile Ile His Trp Arg Tyr Glu Val Lys Ala
420 425 430
Ser Ala Glu Thr Thr Tyr Asp Ser Gly Tyr Met Cys Arg His Glu Ala
435 440 445
Glu Glu Asp Glu Leu Leu Cys Lys Ile Asn Glu Asp Lys Tyr Lys Glu
450 455 460
Met Leu Asp Arg Met Ile Gln Gly Gly Trp Asp Gln Glu Arg Phe Lys
465 470 475 480
Leu His Asn Ile Leu Thr Asp Pro Asn Leu Leu Thr Ile Asp Phe Glu
485 490 495
Lys Asp Ala Tyr Leu Asn Ser Arg Ser Glu Leu Val Leu Pro Asp Tyr
500 505 510
Phe Asp Lys Trp Ile Ser Ser Pro Met Phe Asn Ala Arg Leu Arg Ile
515 520 525
Thr Lys Gly Glu Ile Gly Thr Ser Lys Lys Asp Asp Pro Trp Asn Asn
530 535 540
Arg Ala Val Arg Gly Tyr Ile Lys Ser Pro Ala Glu Ser Leu Asp Phe
545 550 555 560
Val Leu Gly Pro Tyr Tyr Asp Leu Arg Leu Leu Phe Phe Gly Glu Thr
565 570 575
Leu Ser Leu Lys Gln Glu Gln Ser Ala Val Phe Gln Tyr Leu Ser Gln
580 585 590
Leu Asp Asp Phe Pro Ala Leu Thr Gln Leu Thr Gly Asp Ala Val Cys
595 600 605
Pro His Ser Gly Gly Ala Leu Tyr Thr Phe Arg Lys Val Ala Leu Phe
610 615 620
Leu Ile Gly Asn Tyr Glu Lys Leu Ser Pro Asp Leu His Glu Gly Met
625 630 635 640
Glu His Gln Arg Tyr Val His Pro Ser Thr Gly Gly Thr Tyr Gln Lys
645 650 655
Arg Val Leu Glu Met Lys Asp Pro Cys Gln Leu Thr Cys Phe Val Ile
660 665 670
Asp Tyr Ile Phe Glu Lys Arg Glu Gln Leu Arg Asp Thr Lys Glu Ala
675 680 685
Arg Tyr Ile Val Tyr Leu Ile Gln Ser Leu Thr Gly Thr Gln Arg Leu
690 695 700
Ser Val Leu Arg Ser Thr Phe Pro Asn Phe Phe Gln Arg Leu Leu Met
705 710 715 720
Leu Lys Glu Ile Lys Phe Val Arg Asp Leu Asn Val Ile Asn Phe Leu
725 730 735
Pro Leu Met Phe Leu Val His Asp Asn Ile Ser Tyr Trp His Arg Gln
740 745 750
Trp Ser Ile Pro Met Val Leu Phe Asp Asp Thr Ile Lys Leu Ile Pro
755 760 765
Val Glu Val Gly Ala Tyr Ala Asn Arg Phe Gly Phe Lys Ser Phe Met
770 775 780
Asn Phe Thr Arg Phe His Pro Gly Glu Leu Lys Lys Lys Gln Ile Ala
785 790 795 800
Glu Asp Ile His Lys Glu Phe Gly Val Val Ala Phe Glu Tyr Tyr Thr
805 810 815
Asn Thr Lys Ile Ser Gln Gly Asn Val His Thr Pro Val Met Thr Thr
820 825 830
Lys Met Asp Val Leu Arg Val His Leu Ser Ser Leu Cys Ala Gly Leu
835 840 845
Ala Asp Ser Val Val Tyr Thr Leu Pro Val Ala His Pro Lys Lys Cys
850 855 860
Ile Val Leu Ile Ile Val Gly Asp Asp Lys Leu Glu Pro His Thr Arg
865 870 875 880
Ser Glu Gln Ile Val Ser Arg Tyr Asn Tyr Ser Arg Lys His Ile Cys
885 890 895
Gly Ile Val Ser Val Thr Ile Gly Gln Asn Ser Gln Leu Arg Val His
900 905 910
Thr Ser Gly Ile Val Lys His Arg Val Cys Asp Lys Phe Ile Leu Lys
915 920 925
His Lys Cys Lys Val Ile Leu Val Arg Met Pro Gly Tyr Val Phe Gly
930 935 940
Asn Asp Glu Leu Met Thr Lys Leu Leu Asn Val
945 950 955
<210> 172
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV17 CAE53036.1的VP5
<400> 172
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ala Ala Thr Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Thr
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Thr Lys Ile Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Lys Glu Ile Thr Lys Glu Phe Gly Lys Glu Leu Glu
115 120 125
Glu Val Tyr Asp Phe Met Asn Gly Glu Ala Lys Glu Glu Glu Val Val
130 135 140
Gln Glu Gln Tyr Ser Met Leu Cys Lys Ala Val Asp Ser Tyr Glu Lys
145 150 155 160
Ile Leu Lys Ala Glu Asp Ser Lys Met Ala Met Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Ser Glu Arg Ser Gln Asp Glu Ile Lys Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Asn Ala Ile Glu Ile Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Met Arg Ser Pro Lys Ile Glu Pro Thr Ile Ile Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Lys Glu Ile Pro Asp Glu Gln Leu Ala Val Ser Val Leu
290 295 300
Asn Lys Lys Thr Ala Val Thr Asp Asn Cys Asn Glu Ile Ala His Ile
305 310 315 320
Lys Gln Glu Ile Leu Pro Lys Phe Lys Gln Ile Met Asp Glu Glu Lys
325 330 335
Glu Ile Glu Gly Ile Glu Asp Lys Val Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Ile Pro Arg Thr Gln Gln Pro Gln Ile His Ile Tyr Ala
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Arg Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Thr Ser His Trp His Ala Leu Gly Leu
405 410 415
Ala Gln Glu Ala Ser Gly Arg Thr Leu Thr Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ser Ile Ser Ser Thr Tyr Ser Ser Ala Ile His Ala Arg
435 440 445
Arg Met Ile Arg Ser Arg Ala Val His Pro Ile Phe Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Ala Leu Lys Asn Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Glu Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Ile
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 173
<211> 957
<212> PRT
<213> 人工序列
<220>
<223> BTV18 CAE51105.1的VP2
<400> 173
Met Asp Glu Leu Ala Ile Pro Ile Tyr Thr Asp Thr Phe Pro Ala Gly
1 5 10 15
Leu Leu Asp Gly Tyr Asp Tyr Ile Ile Asp Val Ser Thr Arg Val Asp
20 25 30
Glu Pro Lys Gly Arg His Asp Val Thr Glu Ile Pro Lys Arg Ser Met
35 40 45
Phe Asp Ile Lys Asp Glu Pro Ile Arg Glu Ala Ile Ile Tyr Lys Pro
50 55 60
Val Ser Asn Asp Gly Cys Val Leu Pro Arg Val Leu Asp Ile Thr Leu
65 70 75 80
Arg Ala Phe Asp Asp Arg Lys Arg Val Ile Thr Asn Glu Gly His Ser
85 90 95
Glu Phe His Thr Arg Ser Asn Trp Leu Gln Trp Met Ile Asp Asp Ala
100 105 110
Met Asp Tyr Gln Pro Leu Lys Val Asp Val Glu His Val Pro Ser Arg
115 120 125
Ile Ser His Ala Leu Ser Asn Ser Thr Val Arg Leu His Ala Lys Arg
130 135 140
Ala Asp Thr Ala Ser Tyr His Ile Glu Pro Val Glu Val Glu Ala Tyr
145 150 155 160
Gly Cys Asn His Val Trp Thr Glu Arg Ile His His Leu Val Asn Ile
165 170 175
Glu Met Phe His Ser Ser Gln Glu Ala Ala Tyr Ile Leu Lys Pro Thr
180 185 190
Tyr Gln Ile Ile Ala His Ala Glu Arg Ala Ala Val Gly Asp Ala Phe
195 200 205
Asn Gly Arg Thr Leu Glu Leu Asn Arg Asn His Arg Ile Gln Leu Gly
210 215 220
Asp Gln Gly Tyr Val Lys Leu Lys Lys Gly Trp Val Gln Thr Arg Ile
225 230 235 240
Glu Gly Lys Lys Pro Ala Ala Ile Glu Arg Glu Ile Ala Ser Leu Asn
245 250 255
Glu Ile Arg Ala Gln Trp Ile Ala Arg Gly His Asp Arg Arg Glu Ile
260 265 270
Lys Ala Leu Asp Leu Cys Arg Ile Leu Ser Ala Ile Gly Arg Lys Met
275 280 285
Tyr Asn Ile Glu Glu Glu Pro Lys Asn Glu Ala Asp Met Ser Val Lys
290 295 300
Phe Gln Met Glu Leu Asp Glu Ile Phe Lys Pro Gly Asn Ser Glu His
305 310 315 320
Asp Asn Ile Met Gly Gly Gly Thr His Arg Lys Asp Glu Asp Arg Phe
325 330 335
Tyr Val Leu Ile Val Ile Ala Ala Ser Asp Thr Asn Gln Gly Arg Val
340 345 350
Trp Trp Ser Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu Ile Ala Ala
355 360 365
Glu Val Gln Leu Gly Asp Val Tyr Ser Thr Leu Arg Ser Trp Tyr Gln
370 375 380
Trp Ser Ile Arg Pro Ala Tyr Val Pro Tyr Asp Arg Ser Arg Glu Asn
385 390 395 400
Asp Lys Tyr Ile Tyr Thr Arg Thr Asn Leu Phe Asp Ser Ala Leu Gly
405 410 415
Pro Gly Asp Lys Ile Ile His Trp Glu Tyr Gln Leu Leu Asn Glu Thr
420 425 430
Arg Glu Val Ser Met Ser Ser Gly Asn Glu Cys Asp Leu Phe Pro Glu
435 440 445
Asp Glu Glu Phe Thr Thr Lys Phe Asn Glu Glu Arg Tyr Thr Glu Met
450 455 460
Lys Gly Gln Ile Ile Gln Ser Gly Trp Asn His Arg Asp Phe Lys Met
465 470 475 480
His Lys Ile Leu Glu Asp Gly Ala Asn Val Leu Thr Ile Asp Phe Glu
485 490 495
Lys Asp Ala Arg Val Gly Thr Gly Ser Val Leu Thr Leu Pro Asp Tyr
500 505 510
Tyr Asp Lys Trp Ile Val Ala Pro Met Phe Asn Ala Lys Leu Arg Ile
515 520 525
Thr Glu Thr Val Ile Ala Asn Ala His Ser Asp Asp Pro Ala Ile Glu
530 535 540
Arg Ser Val Lys Pro Phe Asp His Asp Pro Ser Thr Leu Gln Arg Phe
545 550 555 560
Cys Leu Ala Arg Tyr Tyr Asp Ile Arg Pro Gly Leu Arg Gly Arg Ala
565 570 575
Leu Leu Lys Gln Gln Asp Gly Ser Thr Ile Thr Lys Lys Val Ser Ala
580 585 590
Leu Asp Asp Tyr Gln Asp Val Val Ser Arg Arg Val Asn Tyr Val Gln
595 600 605
Arg Asp Val Arg Cys Pro Thr Glu Thr Ala Gln Tyr Val Leu Glu Lys
610 615 620
Thr Ser Leu Tyr Leu Leu Ala Leu Phe Asp Lys His Val Met Pro Ser
625 630 635 640
Ala Glu Ser Glu Ala Lys Phe Val His Pro Ser Ile Asp Val Lys His
645 650 655
Asp Ile Glu Thr Trp Arg Ile Ala Asp Ile Ser Gln Leu Ile Val Leu
660 665 670
Ile Phe Asp Tyr Leu Phe Glu Ser Arg Lys Val Val Arg Asp Ala Val
675 680 685
Glu Ala Arg Trp Ile Leu Tyr Lys Ile Arg Ser Ser Thr Gly Arg Ala
690 695 700
Arg Met Glu Val Ile Glu Thr Thr Phe Pro Arg Phe Gly Leu Thr Val
705 710 715 720
Lys Asn Ile Thr Lys Ala Lys Val Asn Gln Asp Ile Ala Cys Leu Asn
725 730 735
Phe Phe Pro Leu Phe Phe Ile Val Gly Asp Asn Met Ala Tyr Ala His
740 745 750
Arg Gln Trp Ser Ile Pro Ile Leu Leu Tyr Ala His Asp Ile Arg Ile
755 760 765
Ile Pro Leu Glu Val Gly Ala Tyr Ser Asn Arg Phe Gly Leu Thr Ser
770 775 780
Tyr Leu Glu Tyr Met Ile Phe Phe Pro Ser Tyr Ala Thr Arg Met Ala
785 790 795 800
Met Ile Asp Glu Ala Asn Lys Glu Cys Ala Ala Ala Leu Leu Asp Tyr
805 810 815
Tyr Leu Ser Thr Asp Val His Arg Gly Gly Val Met Thr Ser Val Ile
820 825 830
Thr Thr Lys Arg Leu Leu Tyr Glu Thr Tyr Leu Ser Ser Leu Cys Gly
835 840 845
Gly Tyr Ser Asp Gly Leu Leu Trp Tyr Ile Pro Ile Thr His Pro Leu
850 855 860
Lys Cys Leu Val Ala Phe Glu Val Ala Asp Asp Val Val Pro Ala Thr
865 870 875 880
Val Arg Lys Glu Arg Ile Leu Met Arg Phe Pro Leu Ser Ala Arg His
885 890 895
Leu Arg Gly Ile Ala Leu Ile Thr Ile Asp Arg Asp Arg Lys Val His
900 905 910
Val Gln Thr Glu Gly Ile Val Ser His Arg Leu Cys Lys Lys Asn Leu
915 920 925
Leu Lys Tyr Val Cys Asp Val Ile Leu Phe Lys Phe Ser Gly Tyr Val
930 935 940
Phe Gly Asn Asp Glu Met Leu Thr Lys Leu Leu Asn Val
945 950 955
<210> 174
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV18 CAE53037.1的VP5
<400> 174
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Leu Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Met Gly Ser
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Met Gln
85 90 95
Thr Lys Ile Arg Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Ile Arg
100 105 110
Leu Lys Tyr Asn Asp Lys Ile Lys Gln Lys Phe Gly Lys Glu Leu Glu
115 120 125
Glu Val Tyr Glu Phe Met Asn Gly Val Ala Lys Gln Glu Glu Asp Glu
130 135 140
Glu Lys His Tyr Asp Val Leu Lys Lys Ala Val Asn Ser Tyr Asp Lys
145 150 155 160
Ile Leu Thr Glu Glu Glu Lys Gln Met Arg Ile Leu Ala Thr Ala Leu
165 170 175
Gln Lys Glu Val Lys Glu Arg Thr Gly Thr Glu Ala Val Met Val Lys
180 185 190
Glu Tyr Arg Asn Lys Ile Asp Ala Leu Lys Glu Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Thr His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Thr Ile Val Ser Thr Val Leu Asp
275 280 285
His Lys Phe Lys Asp Ile Pro Asp Glu Met Leu Ala Val Ser Val Leu
290 295 300
Ser Lys Asn Arg Ala Ile Glu Glu Asn His Lys Glu Ile Ile His Leu
305 310 315 320
Lys Asn Glu Ile Leu Pro Arg Phe Lys Lys Ala Met Asp Glu Glu Lys
325 330 335
Glu Ile Cys Gly Ile Glu Asp Lys Lys Ile His Pro Lys Val Met Met
340 345 350
Lys Phe Lys Ile Pro Arg Thr Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Ile Ser His
370 375 380
His His Ala Asn Glu Ser Phe Phe Ile Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Leu Val His Tyr Glu Asp Leu Thr Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Ala Ala Val Gly Arg Ser Leu Asn Glu Val Tyr Lys Glu Phe
420 425 430
Leu Asn Leu Ala Ile Asn Asn Thr Tyr Ser Ser Gln Met His Ala Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Leu
450 455 460
His Tyr Asp Ile Ser Phe Ser Thr Leu Arg Ser Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Glu Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Ile Lys His Gly Val Lys Ile
500 505 510
Leu Gly Thr Glu Val Asp Ile Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 175
<211> 962
<212> PRT
<213> 人工序列
<220>
<223> BTV19 CAE51106.1的VP2
<400> 175
Met Asp Glu Phe Ala Val Pro Ile Val Arg Gly Arg Thr Ile Pro Tyr
1 5 10 15
Asp Leu Ile Arg Ala Tyr Glu Thr Ile Ile Asp Leu Asp Val Lys Val
20 25 30
Glu Glu Asn Glu Gly Lys His Asp Ile Thr Lys Ile Pro Glu Ser Tyr
35 40 45
Met Cys Asp Val Pro Asp Ile Pro Ile Gln Asp Ala Thr Thr Ala Thr
50 55 60
Leu Ser Arg Asn Asp Gly Tyr Ala Leu Pro Arg Ile Leu Asp Val Ser
65 70 75 80
Ile Ala Ala Tyr Asp Thr Arg Lys Gly Tyr Gln Lys Tyr Asn Gly Ile
85 90 95
Glu Arg Met Thr Asn Gln Val Trp Leu Lys Trp Ala Ile Lys Asp Phe
100 105 110
Met Asp Val Gln Pro Leu Lys Val Ser Leu Thr Asp Ala Glu Gly Lys
115 120 125
Val Arg Tyr Asp Leu Phe Asn Cys Ser Val Arg Met Asp Ala Lys Lys
130 135 140
Ala Glu Ala Val Tyr Tyr Gly Trp Ala Ala Gln Glu Val Ile Asp Lys
145 150 155 160
Gly Cys Asn His Ala Arg Ser Glu Leu Leu Gln His Val Ile Arg Cys
165 170 175
Glu Leu Ser Asn Cys Ala Gln Glu Ser Ala Tyr Met Leu Lys Pro Thr
180 185 190
Gln Lys Leu Glu Pro Val Ala Glu Lys Thr Ser Leu Ser Glu Arg Leu
195 200 205
Leu Pro Gly Ser Ser His Leu Thr Ser Met Arg Arg Gly Glu Val Phe
210 215 220
Arg Met Gly Thr Glu Gln Tyr Asn Arg Met Val Gln Gly Met Val Ser
225 230 235 240
Ser Gln Gly Val Lys Gly His Thr Pro Gly Lys Thr Arg Ile Gln Asp
245 250 255
Glu Met Asn Glu Leu Met Lys Ile Arg Asp Arg Trp Lys Val Ala Arg
260 265 270
Asp Arg Arg Glu Ile Lys Ser Leu Glu Leu Cys Lys Leu Leu Ser Ser
275 280 285
Val Gly Arg Lys Met Tyr Asp Lys Tyr Glu Glu Pro Lys Asp Glu Glu
290 295 300
Ala Met Ser Leu Arg Phe Gln Trp Asn Leu Asp Glu Ile Phe Lys Thr
305 310 315 320
Asp Asn Arg Glu His Ile Asn Ile Ala Val Glu Lys Ser Gly Arg Thr
325 330 335
Asn Asp Glu Arg Phe Phe Ala Leu Ile Met Ile Ala Ala Thr Asp Thr
340 345 350
Asn Arg Gly Arg Ile Trp Trp Ser Asn Met Phe Pro Cys Leu Arg Gly
355 360 365
Ala Leu Ile Val Gly Glu Thr Gln Ser Gly Asp Val Tyr Lys Met Leu
370 375 380
Arg Val Arg Phe Asp Trp Ser Val Arg Asn Glu Tyr Ser Thr Gln Ala
385 390 395 400
Asp Val Ala Arg Gln Asn Asp Asn Tyr Thr Phe Thr Arg Val Asn Leu
405 410 415
Phe Asp Leu Ile Ser Glu Pro Ala Thr Pro Val Ile His Trp Glu Tyr
420 425 430
Asp Leu Asp Ala Pro Arg Lys Thr Asn Tyr Leu Asp Gly His Ala Cys
435 440 445
Asp Glu Phe Pro Asp Asp Tyr Asp Leu Val Cys Ser Phe Asp Glu Thr
450 455 460
Lys Tyr Ser Glu Met Ile Gln Arg Ile Leu Asp Gly Gly Trp Asp His
465 470 475 480
Lys Asn Phe Lys Tyr Tyr Lys Ile Leu Lys Asp Ala Gly Asn Val Leu
485 490 495
Thr Ile Asp Phe Glu Lys Asp Ala Arg Leu Asn Glu His Ser Glu Met
500 505 510
Val Met Pro Ser Tyr Phe Asn Lys Arg Ile Ala Ala Pro Met Phe Arg
515 520 525
Cys Ile Leu Arg Ile Cys Glu Thr Thr Ile Ser Val Arg Gln Asn Ala
530 535 540
Asp Pro Ala Val Ser Arg Thr Ile Arg Pro Leu Lys Ala Lys Ala Ala
545 550 555 560
Asp Leu Leu Asp Phe Thr Leu Gly Ser Tyr Tyr Asp Thr Arg Phe Gly
565 570 575
Val Met Gly Arg Thr Leu Val Met Ser Gln Lys Gln Ser Glu Met Tyr
580 585 590
Lys Met Ile Gln Glu Ala Glu Asp Tyr Ser Glu Leu Met Lys Ser Arg
595 600 605
Gly Val Ile Glu Lys Phe Lys Asn Pro Cys Pro Ile Thr Thr Ala Cys
610 615 620
Gln Tyr Thr Leu Gln Lys Thr Ser Val Leu Ile Leu Ser Arg Ala Gln
625 630 635 640
Arg Tyr Phe Arg Glu Phe His Gly Gly Asp Gln Glu Glu Tyr Met Ser
645 650 655
Pro Thr Leu Asn Phe Glu Trp Ser Asp Met Thr Ser Asn Ile Tyr Asp
660 665 670
Val Ala Gln Met Val Val Leu Val Phe Asp Val Leu Phe Glu Arg Arg
675 680 685
Arg Val Val Arg Gly Val Asp Glu Ser Arg Phe Ile Leu His Gln Leu
690 695 700
Arg Ile Arg Lys Gly Leu Asn Arg Leu Asp Tyr Val Lys Ile His Phe
705 710 715 720
Pro His Leu Tyr Gln Trp Trp Lys Ala Ser Glu Ser Thr Tyr Gly Glu
725 730 735
Ala Gln Lys Leu Asn Leu Leu Pro Leu Leu Phe Leu Val Gly Asp Asn
740 745 750
Phe Ile Tyr Glu His Arg Gln Trp Ala Tyr Pro Ala Val Leu Ile Ser
755 760 765
His Lys Leu Arg Phe Ile Pro Val Glu Leu Gly Ala Tyr Tyr Asn Arg
770 775 780
Phe Gly Leu Thr Ser Leu Ile Ser His Leu Thr Phe His Val Gly Lys
785 790 795 800
Ala Lys Tyr Asp Gln Asn Leu Asp Gln Ala Glu Gln Lys Val Phe Ser
805 810 815
Ala Leu Lys Asp Tyr Tyr Ala Glu Ser Thr Ile Thr Asp Gly Gly Val
820 825 830
Asn Thr His Ser Thr Met Thr Lys Asp Pro Ile Tyr Gln Val Tyr Leu
835 840 845
Ser Thr Ile Cys Gly Gly Leu Leu Asp Ser Leu Val Tyr Leu Met Pro
850 855 860
Ile Thr His Pro Ile Lys Ser Ile Val Ala Ile Val Ile Asn Asp Gly
865 870 875 880
Lys Gly Ser Ala Ser Asp Cys Val Arg Arg Val Thr Arg Lys Leu Arg
885 890 895
Tyr Ile Gln His His Ile Arg Gly Ser Val Val Ile Ser Ile Leu Ser
900 905 910
Asp Gly Ser Ile Asn Thr Tyr Ser Glu Gly Ile Ala Lys Ala Thr Leu
915 920 925
Cys Asp Lys Ser Val Leu Lys Tyr Ser Tyr Lys Val Val Leu Ile Lys
930 935 940
Val Lys Gly His Val Phe Gly His Asp Glu Met Leu Thr Lys Leu Leu
945 950 955 960
Asn Val
<210> 176
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV19 CAE53038.1的VP5
<400> 176
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Val Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Arg Ala Ala Glu Arg Phe Ala Glu Ser Glu Val Gly Ala Ala Thr Ile
35 40 45
Asp Gly Phe Val Gln Gly Ser Val His Ser Ile Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ser
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Thr Lys Leu Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Leu Asn Lys Lys Ile Val Asp Lys Phe Gly Thr Glu Leu Glu
115 120 125
Glu Val Tyr Asp Phe Met Asn Gly Ala Ala Lys Asp Asp Glu Asn Glu
130 135 140
Ile Lys Gln Tyr Asp Ile Leu Cys Lys Ala Val Asp Ser Tyr Gly Lys
145 150 155 160
Ile Leu Arg Glu Glu Asp Lys Lys Met Leu Glu Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ser Glu Ser Arg Ser Asp Glu Glu Thr Lys Met Val Arg
180 185 190
Glu Tyr Arg Ser Lys Ile Asp Ala Leu Arg Asp Ala Val Asp Val Glu
195 200 205
Arg Glu Ser Met Thr Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ser Asp Val Leu Glu Ala Ala Ala Glu Glu Val Pro Leu Val Gly Ser
225 230 235 240
Gly Met Ala Thr Ala Ile Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Thr His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Ser Leu Ile Ala Thr Thr Leu Glu
275 280 285
Tyr Arg Asp Arg Pro Ile Pro Asp Asp Ser Leu Ala Val Ser Val Leu
290 295 300
Glu Lys Arg Arg Ala Ile Glu Glu Asn Lys Asn Glu Ile Glu His Ile
305 310 315 320
Lys Asn Glu Ile Leu Pro Arg Phe Lys Lys Ala Met Glu Glu Glu Lys
325 330 335
Glu Ile Ser Gly Ile Glu Glu Ser Val Ile His Pro Lys Val Met Met
340 345 350
Arg Phe Lys Ile Pro Arg Ser Gln Gln Pro Gln Ile His Ile Tyr Cys
355 360 365
Ser Pro Trp Asp Ser Asp Thr Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Met Asn Glu Ser Phe Phe Ile Gly Phe Asp Leu Glu Leu Asp
385 390 395 400
Leu Val His Phe Glu Asp Leu Thr Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Thr Val Val Gly Arg Thr Phe Gln Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Gly Arg Ala Met Met Asp Pro Ser Ser Gly Ala Met His Lys Arg
435 440 445
Arg Met Ser Arg Ser Arg Ser Val His Pro Ile Tyr Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Ser Tyr Ala Thr Leu Lys Ser Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Val Leu Arg Gly Pro Ile His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys His Gly Ile Lys Ile
500 505 510
Leu Asn Asp Lys Ile Asp Leu Thr Ser Leu Leu Arg Asn Ala
515 520 525
<210> 177
<211> 956
<212> PRT
<213> 人工序列
<220>
<223> BTV20 CAE51107.1的VP2
<400> 177
Met Glu Glu Phe Val Ile Pro Val Phe Ser Glu Gln Asp Met Pro Tyr
1 5 10 15
Ala Leu Ile Asn Gln Tyr Pro Leu Ala Ile Arg Thr Asp Val Lys Ile
20 25 30
Pro Asp Val Glu Gly Ile His Asp Leu Ala Lys Ile Pro Glu Ser Asp
35 40 45
Met Ile Asp Val Pro Lys Leu Asp Ile Val Ser Ala Leu Asn Tyr Lys
50 55 60
Pro Val Arg Asn Asp Gly Ile Val Val Pro Arg Leu Leu Asp Ile Thr
65 70 75 80
Leu Lys Ala Tyr Asp Asp Arg Lys Ser Thr Lys Asp Ala Gln Gly Ile
85 90 95
Lys Phe Met Thr Asn Ala Gly Trp Met Lys Trp Ala Ile Asp Asp Arg
100 105 110
Met Asp Ile Gln Pro Leu Lys Val Lys Leu Asp Gln His Tyr Ser Ile
115 120 125
Asp Tyr Gln Leu Phe Asn Cys Val Val Lys Ala Lys Thr Ala Asn Ala
130 135 140
Asp Thr Met Tyr Tyr Asp Tyr Phe Pro Ile Glu Asn Lys Thr Lys Lys
145 150 155 160
Cys Asn His Thr Asn Leu Asp Leu Leu Arg Ser Leu Thr Ala Val Glu
165 170 175
Met Phe His Val Leu Gln Gly Ala Ala Tyr Asn Leu Lys Ala Asn Tyr
180 185 190
Glu Leu Ile Ala Asn Ser Glu Arg Glu Ser Leu Glu Glu Ser Tyr Pro
195 200 205
Ile Gly Ser Gln Lys Trp Val His Leu Thr Arg Gly Thr Lys Ile Gly
210 215 220
Asn Ser Gly Val Pro Tyr Glu Arg Phe Ile Ser Ser Met Val Gln Val
225 230 235 240
Val Val Lys Gly Lys Val Pro Val Glu Ile Val Ser Glu Val Val Arg
245 250 255
Leu Asn Glu Ile Arg Thr Glu Trp Ile Asn Ala Gly Phe Asp Arg Ser
260 265 270
Arg Ile Arg Ala Leu Glu Leu Cys Asn Ile Leu Ser Thr Ile Gly Arg
275 280 285
Lys Met Met Asp Thr Gln Glu Glu Pro Lys Asp Glu Met Asp Leu Ser
290 295 300
Thr Arg Phe Gln Phe Lys Leu Asp Asp Lys Phe Asn Thr Ala Asp Ser
305 310 315 320
Glu Tyr Ile Val Ile Ser Arg Val Gly Gly Ala Ala Thr Asn Glu Gly
325 330 335
Arg Phe Tyr Ala Leu Ile Ala Ile Ala Ala Thr Asp Thr Gln Arg Gly
340 345 350
Arg Val Trp Arg Thr Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu Ile
355 360 365
Ala Ala Glu Cys Glu Leu Gly Asp Val Tyr Tyr Ser Leu Arg His Thr
370 375 380
Tyr Gln Trp Ser Leu Arg Pro Glu Tyr Asp Gln Arg Glu Arg Ser Leu
385 390 395 400
Glu Asn Asn Lys Tyr Val Phe Ser Arg Ile Asn Leu Phe Asp Ser Asn
405 410 415
Leu Thr Val Gly Asp Lys Val Ile His Trp Arg Tyr Asp Leu Thr Lys
420 425 430
Pro Met Asp Thr Thr Phe Asp Asp Gly Tyr Met Cys Val Ser His Glu
435 440 445
Ile Asp Gly Glu Leu Leu Cys Arg Ile Asp Asp Asp Arg Tyr Lys Glu
450 455 460
Met Leu Glu Arg Met Ile Gln Gly Gly Trp Asp Gln Glu Arg Phe Lys
465 470 475 480
Leu His Ser Ile Leu Thr Glu Pro Asn Leu Leu Thr Ile Asp Phe Glu
485 490 495
Lys Asp Ala Tyr Leu Asn Ser Arg Ser Glu Leu Val Met Pro Asp Tyr
500 505 510
Phe Asp Lys Trp Ile Asn Ser Pro Met Phe Asn Ala Arg Leu Arg Leu
515 520 525
Thr His Gly Glu Ile Ala Ser Arg Lys Thr Asp Asp Pro Trp Asn Asn
530 535 540
Arg Val Val Phe Gly Tyr Ile Lys Thr Thr Thr Glu Ser Leu Asp Tyr
545 550 555 560
Met Leu Gly Arg Tyr Tyr Asp Val Arg Leu Gln Phe Tyr Gly Asp Thr
565 570 575
Leu Ser Gln Gly Gln Arg Gln Ser Ser Val Phe Ala Tyr Ile Ser Gln
580 585 590
Arg Asp Asp Phe Gln Thr Leu Thr His Tyr Val Lys Asp Asp Ala Ile
595 600 605
Cys Pro His Ala Gly Gly Ser Ile Tyr Thr Phe Arg Lys Thr Ala Leu
610 615 620
Met Leu Met Ala Asn Tyr Glu Lys Leu Ser Pro Glu Leu His Glu Gly
625 630 635 640
Leu Glu His Gln Thr Tyr Thr His Pro Ser Asp Gly Met Asp Tyr Arg
645 650 655
Lys Tyr Val Val Asp Met Lys Asp Leu Ser Gln Leu Ile Cys Phe Val
660 665 670
Phe Asp Tyr Ile Phe Glu Lys Arg Ala Gln Leu Arg Asp Pro Lys Glu
675 680 685
Ala Arg Arg Ile Leu Tyr Leu Val Gln Asn Asn Val Gly Ser Gln Arg
690 695 700
Leu Gly Val Leu Ser Ser Ala Phe Pro Asn Leu Ala Asn Arg Leu Leu
705 710 715 720
Lys Leu Arg Glu Ile His Phe Ile Tyr Asp Met Asn Val Ile Asn Phe
725 730 735
Leu Pro Leu Leu Phe Leu Val Gln Asp Asn Ile Ser Tyr Trp His Arg
740 745 750
Gln Trp Ala Ile Pro Met Val Leu Phe Asp Glu Thr Ile Lys Leu Ile
755 760 765
Pro Val Glu Val Gly Ala Tyr Ala Asn Arg Phe Gly Phe Lys Ser Phe
770 775 780
Phe Asn Phe Thr Arg Phe His Pro Gly Asp Ser Arg Lys Lys Gln Ile
785 790 795 800
Ala Asp Asp Val His Lys Glu Phe Gly Thr Ile Cys Tyr Glu Tyr Tyr
805 810 815
Met Asn Thr Arg Met Ser Gln Gly Gly Val Asn Ile Ser Val Val Thr
820 825 830
Thr Lys Leu Asp Val Leu Lys Ile His Leu Ala Ser Leu Cys Ala Gly
835 840 845
Leu Ala Asp Ser Ile Val Tyr Thr Leu Pro Val Ala His Pro Arg Lys
850 855 860
Cys Ile Val Leu Ile Val Val Gly Asp Asp Lys Leu Glu Pro Gln Ile
865 870 875 880
Arg Ala Glu Gln Val Leu Ser Lys Tyr Tyr Tyr Ser Arg Lys His Val
885 890 895
Leu Gly Val Ile Ser Ile Ser Val Ser Gln Asp Gly Lys Leu Lys Val
900 905 910
Tyr Ser Ser Gly Ile Val Arg His Arg Leu Cys Glu Lys Ser Ile Leu
915 920 925
Lys Tyr Lys Cys Lys Thr Ile Leu Ile Arg Met Pro Gly His Val Phe
930 935 940
Gly Asn Asp Glu Leu Met Thr Lys Leu Leu Asn Ile
945 950 955
<210> 178
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV20 CAE53039.1的VP5
<400> 178
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ala Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Ser Ala
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Met Gln
85 90 95
Ala Lys Ile Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Ile Arg
100 105 110
Leu Lys Tyr Asn Lys Glu Ile Thr Asp Ala Phe Gly Lys Glu Leu Asn
115 120 125
Glu Val Tyr Glu Phe Met Asn Gly Glu Thr Lys Glu Glu Glu Val Val
130 135 140
Gln Glu Gln Tyr Ser Met Leu Cys Lys Ala Val Asp Ser Tyr Glu Lys
145 150 155 160
Ile Leu Lys Ala Glu Asp Ser Lys Met Ala Val Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Ser Glu Arg Ser Glu Asp Glu Ile Lys Met Val Lys
180 185 190
Glu Tyr Arg His Lys Ile Asp Ala Leu Lys Asn Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Val Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Met Arg Thr Pro Lys Ile Glu Pro Thr Ile Ile Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Lys Asp Ile Pro Asp Asp Arg Leu Ala Ile Ser Val Leu
290 295 300
Asn Lys Lys Ala Ala Val Ala Asp Asn Cys Asn Glu Ile Ser His Ile
305 310 315 320
Lys Gln Glu Ile Leu Pro Lys Phe Arg Lys Ile Met Asp Glu Glu Lys
325 330 335
Glu Ile Glu Gly Ile Glu Asp Lys Val Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Ile Pro Arg Thr Gln Gln Pro Gln Ile His Ile Tyr Ala
355 360 365
Ala Pro Trp Asp Ser Asp Glu Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Arg Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Thr Ser His Trp His Ala Leu Gly Asn
405 410 415
Ala Gln Glu Ala Thr Gly Arg Thr Leu Thr Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ser Ala Thr Ser Met Phe Ser Ser Val Ile His Thr Arg
435 440 445
Arg Met Ile Arg Ser Arg Ala Val His Pro Ile Phe Leu Gly Ser Met
450 455 460
His Tyr Asp Val Thr Tyr Glu Val Leu Lys Asn Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Ile
500 505 510
Leu Gly Asp Lys Leu Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 179
<211> 955
<212> PRT
<213> 人工序列
<220>
<223> BTV21 CAE51108.1的VP2
<400> 179
Met Asp Glu Leu Ala Ile Pro Ile Ile Asp Gly Arg Phe Pro Lys Glu
1 5 10 15
Ala Leu Leu Glu Tyr Cys Cys Ile Val Glu Leu Asn Pro Ala Ser Glu
20 25 30
Glu Asp Asp Val Asp Val Thr Thr Ile Pro Gln Lys Asp Met Val Asp
35 40 45
Ile Pro Asp Ala Pro Ile Arg Glu Ala Leu Asn Tyr Leu Pro Lys Arg
50 55 60
Asn Asn Gly His Val Leu Thr Arg Val Val Asp Ile Ser Leu Ser Gly
65 70 75 80
Tyr Asp Arg Arg Lys Tyr His Arg Thr Ser Thr Gly Gly Asp Phe Leu
85 90 95
Ser Pro Glu Glu Trp Leu Asn Trp Met Ile Arg Asp Ser Val Asp Val
100 105 110
Gln Pro Leu Lys Ala Ser Leu Glu Phe Pro Ser Lys Ile Lys His Ser
115 120 125
Leu Phe Ser Ser Asn Val Tyr Val Ser Val Lys Lys Ala Asp Thr Ile
130 135 140
Ser Tyr His Ile Glu Ala Lys Glu Val Glu Thr Arg Ala Cys Asp His
145 150 155 160
Ala Arg Val Ala Met Trp Asn Asn Leu Val Arg His Glu Met Met His
165 170 175
Ser Ala Gln Glu Pro Ala Tyr Ile Leu Lys Pro Thr Leu Asp Ile Ile
180 185 190
Val His Ser Glu Arg Ala Ser Thr Asp Val Pro Phe Glu Leu Val Gly
195 200 205
Gln Arg Tyr Ile Ser Ile Gly Lys Asn His Arg Ile Val Leu Gly Asp
210 215 220
Asp Ala Tyr Arg Lys Thr Leu Asp Gly Leu Thr Arg Leu Arg Val Gln
225 230 235 240
Gly Arg Ile Pro Glu Leu Ile Arg Asn Glu Ile Gln Gln Leu His Gln
245 250 255
Ile Arg Asp Ala Trp Ile Thr Asp Ser Tyr Asn Pro Arg His Ile Arg
260 265 270
Ser Leu Glu Leu Cys Arg Ile Leu Ser His Ile Gly Arg Lys Met Val
275 280 285
His Ile Glu Glu Glu Pro Lys Asp Glu Ser Asp Leu Ser Val Lys Phe
290 295 300
Gln Phe Lys Leu Asp Glu Lys Phe Arg Ile Asn Asp Asp Glu Arg Asn
305 310 315 320
Val Ile Phe Thr Ser Lys Gly Gln Arg Asn Asp Gln Gln Arg Phe Phe
325 330 335
Val Leu Ile Met Ile Ala Ala Ser Asp Thr Tyr Asn Ser Arg Ile Trp
340 345 350
Trp Thr Asn Pro Tyr Pro Cys Leu Arg Gly Ala Leu Ile Ala Ser Glu
355 360 365
Thr Ser Leu Gly Asp Val Tyr Trp Thr Leu Arg His Phe Tyr Asp Trp
370 375 380
Ser Val Arg Pro Val Tyr Ser Pro Arg Gln Lys Glu Arg Glu Asp Asp
385 390 395 400
Lys Tyr Leu Tyr Gly Arg Val Asn Leu Phe Asp Leu Asp Ala Asp Pro
405 410 415
Gly Thr Gln Ile Ile His Trp Glu Tyr Lys Pro Ile Thr Ser Thr Val
420 425 430
Lys Thr Thr Tyr Glu Arg Gly Asn Met Cys Asp Leu Phe Pro Asp Val
435 440 445
Asp Gly Ile Thr Thr Lys Phe Asn Lys Thr Ala Tyr Ile Asp Met Ile
450 455 460
Ser Glu Leu Leu Asn Gly Gly Trp Asn Thr Arg Glu Phe Lys Met Tyr
465 470 475 480
Lys Ile Leu Glu Ser Val Gly Asn Val Leu Thr Ile Asp Phe Glu Lys
485 490 495
Asp Thr Lys Leu Asn Ser Arg Ser Glu Leu Ser Leu Pro Tyr Tyr Tyr
500 505 510
Asp Lys Trp Ile Tyr Ala Pro Met Phe Asn Ala Arg Leu Lys Ile Thr
515 520 525
Gln Thr Glu Ile Ala Gln Arg Lys Ala Asp Asp Pro Ser Ile Tyr Arg
530 535 540
Thr Leu Ala Pro Ile Ser Ser Asp Pro Ile Asp Leu Gln Arg Met Cys
545 550 555 560
Leu Ala Asn Tyr Tyr Asp Val Arg Pro Ala Met His Gly Arg Ala Leu
565 570 575
Ser Lys Arg Gln Glu Gln Ser Ala Tyr His Lys Asn Leu Ala Asn Leu
580 585 590
Ala Asp Tyr Gln Asp Ile Leu Lys Arg Arg Gly Tyr Arg Lys Val Glu
595 600 605
Asn Pro Cys Pro Leu Ile Thr Cys Ser Tyr Ile Leu Glu Lys Ser Ala
610 615 620
Leu Leu Trp Ile Asp Ile Met Glu Lys His Val Gly Tyr Ala Pro Glu
625 630 635 640
Ser Glu Ile Glu Phe Asp Phe Pro Lys Val Asn Thr Ser Thr Arg Ile
645 650 655
Gly Thr Glu Arg Ile Val Asp Ile Ser Gln Leu Val Val Leu Met Ile
660 665 670
Asp Trp Leu Tyr Glu Lys Arg Gln Thr Thr Arg Ser Val Asp Glu Ala
675 680 685
Arg Trp Thr Val Gln Val Ile Arg Arg Val Thr Gly Lys Lys Arg Leu
690 695 700
Glu Ala Ile Ser Arg Glu Phe Pro Asn Phe Gly Glu Ala Val Ser Arg
705 710 715 720
Leu Ala Cys Pro Lys Thr Val Arg Asp Val Met Val Ile Asn Phe Leu
725 730 735
Pro Phe Leu Phe Leu Leu Gly Asp Asn Met Ala Tyr Glu His Arg Gln
740 745 750
Trp Ser Val Pro Ile Ile Leu Tyr Ala Asp Thr Leu Trp Phe Leu Pro
755 760 765
Val Glu Val Gly Ala Tyr Tyr Asn Arg Phe Gly Ile Val Ser Phe Leu
770 775 780
Glu Tyr Leu Thr Phe Phe Pro Ser Asn His Met Arg Thr Val Ala Leu
785 790 795 800
Asp Glu Ala Glu Ser Lys Val Gly Lys Leu Ile Leu Glu Phe Tyr Leu
805 810 815
Asn Thr Thr Ile Tyr Leu Gly Asn Ile Gln Gln Ser Val Ile Ser Thr
820 825 830
Lys Gln Ile Leu Tyr Glu Thr Tyr Leu Ser Ser Leu Cys Gly Gly Phe
835 840 845
Thr Glu Gly Leu Val Trp Tyr Leu Pro Ile Thr His Pro Gln Lys Cys
850 855 860
Val Val Ala Ile Glu Ile Cys Asp Asp Arg Val Ser Ala Gly Tyr Arg
865 870 875 880
Cys Asp Lys Leu Arg Ser Arg Phe Pro Leu Ser Leu Asp His Leu Arg
885 890 895
Gly Ile Val Ile Leu Ser Val Ser Lys Ser Arg Ala Ile Ser Ala Tyr
900 905 910
Ser Glu Gly Ile Val Ser His Arg Leu Cys Lys Lys Asn Val Leu Gly
915 920 925
Phe Thr Cys Arg Val Leu Leu Leu Lys Phe Ser Gly His Val Phe Gly
930 935 940
Asn Asp Glu Met Leu Thr Lys Leu Leu Asn Val
945 950 955
<210> 180
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV21 CAE53040.1的VP5
<400> 180
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Ile Val Gln Gly Ser Val His Ser Ile Met Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ala
65 70 75 80
Gly Asp Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Met Lys Ile Lys Glu Ile Glu Glu Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Gly Lys Glu Ile Thr Lys Lys Phe Gly Glu Glu Leu Glu
115 120 125
Glu Ile Tyr Gln Phe Met Asn Gly Glu Val Lys Asp Glu Glu Glu Gln
130 135 140
Glu Glu Gln Tyr Lys Val Leu Cys Lys Ala Val Asn Ser Tyr Glu Lys
145 150 155 160
Leu Leu Ile Ala Glu Asn Asp Lys Met Arg Ile Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Ala Glu Arg Thr Glu Ala Glu Ser Thr Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Ala Ala Ile Glu Ile Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Val Gly Ser
225 230 235 240
Gly Met Ala Thr Ala Ile Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Thr Met Val Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Glu Asp Ile Pro Asp Lys Lys Leu Ala Met Ser Ile Leu
290 295 300
Ala Lys Asn Asn Ala Ile Thr Ala Asn Thr Arg Glu Ile Gln His Ile
305 310 315 320
Lys Glu Glu Ile Leu Pro Lys Phe Lys Lys Val Met Asp Glu Glu Lys
325 330 335
Glu Leu Glu Gly Ile Asp Asp Lys Lys Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Val Pro Arg Ser Gln Gln Pro Gln Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
Phe His Ala Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Ala Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Glu Ala Lys Gly Arg Thr Leu Asn Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ala Ile Gly Ser Thr Tyr Thr Ser Pro Met His Ala Arg
435 440 445
Arg Met Ile Arg Ser Lys Thr Val Tyr Pro Ile Tyr Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Asp Thr Leu Lys Ser Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Val
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Lys Asn Ala
515 520 525
<210> 181
<211> 951
<212> PRT
<213> 人工序列
<220>
<223> BTV22 CAE51109.1的VP2
<400> 181
Met Glu Asp Phe Pro Ile Cys Ala Ile Glu Arg Glu Ser Val Tyr Asp
1 5 10 15
Glu Asp Phe Ile Met Arg Tyr Pro Val Ile Val Asp Leu Lys Glu Cys
20 25 30
Val Ala Asp Arg Arg Asn Lys Thr Asp Val Ser Lys Leu Glu Glu Ile
35 40 45
Lys Gly Val Cys Val Arg Ser Glu Gly Val Arg Val Ala Leu Thr Ser
50 55 60
Arg Pro Asp Pro Leu Ser His Val Ile Thr Pro Ser Ala Leu Asn Val
65 70 75 80
Leu Ile Gln Ala Tyr Asp Arg Lys His Lys Val Ser Leu Arg Lys Arg
85 90 95
Phe Glu Asp Gly Glu Gly Val Thr Glu Tyr Asp Leu Asp Thr Trp Val
100 105 110
Lys Lys Arg Leu Glu Glu Gln Ala Asp Leu Gln Ala Val Tyr His Thr
115 120 125
Ile Asp Ala Asn Arg Gly Lys Leu Lys Phe Lys Thr Leu Met Gly Thr
130 135 140
Ile Arg Leu Asp Ser Asn Tyr Ala Glu Thr Val Gly Tyr Asp Tyr Ala
145 150 155 160
Pro Val Ser Lys Ala Gly Cys Asn His Gly Asn Glu Asp Pro Leu Tyr
165 170 175
Asp Phe Ile Leu Ser Gly Ala Phe Gln Val Ser Lys Thr Ala Gly Tyr
180 185 190
Thr Leu Lys Gln Thr Tyr Arg Leu Thr Val Arg Thr Glu Val Arg Glu
195 200 205
Asn Asn Lys Asn Glu Leu Val Val Asn Glu Ile Tyr Thr Pro Gly Ile
210 215 220
Val Arg Gly Glu Gln Ile Arg Asp Met Thr Ser Val Ala Tyr Met Arg
225 230 235 240
Phe Arg Asp Gly Trp Ile Arg Ala Ile Ile Gln Pro Gln Ile Val Glu
245 250 255
Glu Leu Arg Ala Ser Lys Asp Ala Leu Asp Arg Ile Ser Asn Glu Trp
260 265 270
Tyr Ser Ser Arg Asn Thr Pro Asp Val Val Glu Ile Cys Arg Ile Val
275 280 285
Ser Ser Ile Gly Arg Arg Met Trp Asn Ser Glu Glu Thr Pro Val Asp
290 295 300
Asp Ala Met Arg Ser Arg Val Phe Gln Glu Lys Leu Arg Leu Leu Phe
305 310 315 320
Arg Val Gly Asn Ser Glu Tyr Asp Thr Ile Gln Ser Ile Ser Ser Gly
325 330 335
Gln Thr Asp Leu Lys Lys Phe Tyr Ala Leu Leu Ala Ile Ala Ala Thr
340 345 350
Asp Ser Tyr Arg Trp Arg Ile Trp Trp Ser Asn Pro Tyr Pro Cys Leu
355 360 365
Arg Gly Thr Ile Ile Ala Cys Glu Met Glu Leu Gly Asp Val Tyr Lys
370 375 380
Thr Leu Arg Ser Thr Phe Arg Trp Ser Leu Arg Pro Glu Tyr Ala Asn
385 390 395 400
Arg Ser Glu Ile Asp Arg Glu Lys Lys Ala Tyr Pro Tyr Gln Arg Ile
405 410 415
Asn Leu Phe Glu Ser Lys Leu Thr Pro Gly Thr Arg Ile Ile Asn Trp
420 425 430
Leu Val Thr Arg Val Pro Leu Glu Lys Pro Asp Ile Ser Thr Gly Arg
435 440 445
Val Cys Met Thr Ser Thr Glu Ser Asn Tyr Ile Leu Lys Ile Asp Asp
450 455 460
Ala Arg Tyr Lys Gln Met Ile Ser Gln Ile Ile Glu Arg Gly Trp Glu
465 470 475 480
Lys Glu Gln Leu Lys Phe Glu Lys Ile Val Gln Asp Asp Gly Asn Val
485 490 495
Phe Lys Met Glu Phe Glu Lys Asp Ala Cys Met Asp Glu Lys Ser His
500 505 510
Leu Val Met Pro Tyr Tyr Tyr Asp Lys Glu Ile His Cys Pro Ile Tyr
515 520 525
His Gln Lys Ile Lys Ile Thr Glu Ala Gln Met Ala Asn Glu Trp Ser
530 535 540
Asp Asp Pro Trp Val Asn Arg Val Val Ser Gly Tyr Leu Gly Asp His
545 550 555 560
Val Glu Thr Tyr Gly Leu Gly Tyr Asn His Ile Phe Asp Thr Arg Val
565 570 575
Pro Leu Lys Gly Asn Leu Leu Glu Gln Glu Gln Glu Ile Ser Ser Tyr
580 585 590
His Glu His Leu Arg Ser Asp Glu Ser Lys Glu Asp Asp Thr His Cys
595 600 605
Ala Leu Arg Ile Phe Ser Asp Lys Ala Ile Arg Met Leu Phe Ile Ala
610 615 620
Ile Tyr Lys Lys Leu Ile His His Ile Pro Lys Glu Glu Tyr Ala Thr
625 630 635 640
Arg Glu Glu Glu Leu Ile Gln Tyr Ile Ser Met Val Glu Arg Val Arg
645 650 655
Ser Trp Asp Gln Phe Ile Val Phe Ile Phe Asp Phe Phe Phe Glu Asn
660 665 670
Arg Lys Gly Ile Arg Thr Met Glu Glu Ala Thr Gln Leu Ala Lys Ser
675 680 685
Ile Arg Gly Ser Val Gly Ala Met Arg Trp Thr Ile Phe Ser Ser Ser
690 695 700
Phe Pro Thr Ser Tyr Arg Leu Met Lys Gln Ile Leu Val Ala Lys Arg
705 710 715 720
Trp Gly Asp Cys Tyr Ile Met Asn Phe Leu Pro Leu Leu Ile Cys Gly
725 730 735
Gly Thr Ser Ile Ser Tyr Leu His Arg Glu Trp Ser Tyr Pro Met Ile
740 745 750
Val Thr Phe Glu Asp Gly Ile Lys Ile Val Pro Thr Met Val Gly Arg
755 760 765
Ser Leu Pro Gln Gly Ser Leu Gly Glu Trp Val Ser Phe Ile Ser Phe
770 775 780
Phe Met Gly Thr Val His Glu Lys Ile Lys Val Glu Glu Asp Glu Leu
785 790 795 800
Glu Val Leu Arg Arg Cys Val Glu Phe Tyr Arg Asp Val Lys Met Val
805 810 815
Asp Val Ile Lys Asp Thr Pro Val Arg Met Thr Lys Tyr Ile Thr Cys
820 825 830
Asp Leu Ala Phe Gly Ser Gly Cys Gly Gly Ile Ser Glu Ser Ile Ser
835 840 845
Phe Ile Leu Pro Val Thr His Pro Asn Arg Ser Leu Val Val Phe Val
850 855 860
Ile Ser Asp Asp Lys Leu Ser Pro Ser Gln His Ala Ala Arg Ala Lys
865 870 875 880
Arg Arg Phe Lys Gly Thr Cys Gln Tyr Ile Asp Asp Val Ile Val Leu
885 890 895
Gln Met Lys Arg Pro Gly Leu Val Gly Ser Val Trp Ser Glu Lys His
900 905 910
Ser Lys Ile Lys Val Cys Arg Arg Asn Phe Leu Asn Tyr Asp His Lys
915 920 925
Val Ile Leu Thr Lys Phe Ala Gly Leu Val Tyr Gly Asn Tyr Glu Leu
930 935 940
Leu Thr Lys Leu Ala Asn Leu
945 950
<210> 182
<211> 529
<212> PRT
<213> 人工序列
<220>
<223> BTV22 CAE53041.1的VP5
<400> 182
Met Met Gly Lys Ile Ile Lys Ser Leu Gly Lys Phe Gly Lys Lys Val
1 5 10 15
Gly Asn Ala Leu Thr Ser Lys Thr Ala Gln Lys Ile Tyr Lys Thr Ile
20 25 30
Gly Asn Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala
35 40 45
Ile Asp Gly Leu Ile Gln Gly Ser Val His Ser Ile Met Thr Gly Glu
50 55 60
Ser Tyr Gly Glu Ser Val Lys Gln Ala Val Ile Leu Asn Val Leu Gly
65 70 75 80
Gly Gly Asp Thr Ile Ala Asp Pro Leu Ser Pro Gly Glu Asn Glu Ile
85 90 95
Tyr Arg Lys Leu Arg Gln Leu Glu Glu Glu Gln Lys Gly Glu Val Val
100 105 110
Arg Thr Lys His Asn Thr Glu Ile Leu Asp Lys Phe Gly Arg Asp Leu
115 120 125
Asp Glu Val Tyr Lys Phe Ala Thr Gln Glu His Lys Val Gly Glu Asp
130 135 140
Glu Glu Asp Gln Ile Lys Met Leu Glu Lys Ala Leu Lys Ser Tyr Val
145 150 155 160
Gly Val Phe Lys Ala Glu Asn Glu Ser Ile Asn Lys Leu Lys Gln Ala
165 170 175
Leu Tyr Leu Glu Ser Thr Glu Arg Thr Asp Glu Glu Thr Lys Met Ile
180 185 190
Ser Glu Tyr Arg Asp Lys Ile Asp Ala Leu Arg Gly Ala Ile Glu Val
195 200 205
Glu Lys Glu Gly Leu Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met
210 215 220
Ser Ala Asp Val Leu Glu Ala Ala Ala Glu Glu Val Pro Leu Phe Gly
225 230 235 240
Ala Gly Val Ala Thr Ser Ile Ala Thr Ala Arg Ala Ile Glu Gly Ala
245 250 255
Tyr Lys Ile Lys Ala Val Ile Ser Ala Leu Ser Gly Ile Asp Leu Thr
260 265 270
His Leu Ser Val Pro Lys Val Gln Pro Lys Thr Leu Glu Ala Ile Leu
275 280 285
Asp Thr Pro Lys Gly Lys Asp Ile Ser Asp Leu Arg Leu Thr Glu Gly
290 295 300
Leu Ile Ala Lys Met Asp Lys Ile Ser Glu Asn Ile Ser Glu Val Asp
305 310 315 320
His Ile Gln Asn Val Ile Val Pro Lys Leu Lys Lys Ala Ala Glu Glu
325 330 335
Asp Lys Glu Asn Ile Thr Asn Trp Ser Glu Lys Arg Ile His Pro Lys
340 345 350
Thr Val Gln Arg Phe Lys Ile Pro Gln Ala Gln Arg Pro Ser Met His
355 360 365
Ile Tyr Ala Ala Pro Trp Asp Ser Asp Glu Val Phe Ile Phe His Ile
370 375 380
Ile Ser Pro His His Lys Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu
385 390 395 400
Glu Ile Glu Tyr Val Phe Tyr Glu Asp Leu Thr Gln His Trp His Ala
405 410 415
Leu Gly Ala Ala Gln Ser Ala Tyr Gly Arg Thr Phe Arg Glu Ala Tyr
420 425 430
Arg Glu Phe Phe Met Leu Ala Ser Asn Val Ala Gly Gln Gly Ala Ile
435 440 445
His Gln Lys Arg Leu Leu Arg Ser Arg Ser Ser His Pro Ile Tyr Leu
450 455 460
Gly Met His Asp Tyr Glu Val Ser Tyr Glu Gln Ile Lys Met Asn Ala
465 470 475 480
Met Ala Leu Val Ser Asn Glu Asp Leu Gln Met His Val Leu Arg Gly
485 490 495
Pro Val His Phe Gln Arg Arg Thr Ile Val Ala Ala Leu Lys Tyr Gly
500 505 510
Ile Lys Ile Leu Thr Gln Pro Asp Asp Val Ser Val Met Leu Arg Asn
515 520 525
Ala
<210> 183
<211> 957
<212> PRT
<213> 人工序列
<220>
<223> BTV23 CAE51110.1的VP2
<400> 183
Met Asp Glu Leu Ala Ile Pro Ile Tyr Thr Asn Val Phe Pro Ala Glu
1 5 10 15
Leu Leu Asp Gly Tyr Asp Tyr Ile Ile Asp Val Ser Thr Arg Ile Asp
20 25 30
Glu Pro Ala Gly Arg His Asp Val Thr Gln Ile Pro Asn Ser Ala Met
35 40 45
Phe Asp Val Gln Asn Glu His Ile Arg Asp Ala Ile Ile Ser Lys Pro
50 55 60
Val Ser Asn Asp Gly Tyr Val Leu Pro Arg Ile Leu Asp Ile Ser Leu
65 70 75 80
Arg Ala Phe Asp Asp Arg Lys Arg Ile Ile Thr Ser Gly Gly Gln Ser
85 90 95
Glu Tyr His Thr Lys Ala Asp Trp Leu Gln Trp Met Ile Asp Asp Ala
100 105 110
Met Asp Val Gln Pro Leu Lys Val Asp Thr Ala His Thr Arg Thr Arg
115 120 125
Val Ser His Ala Leu Phe Asn Cys Thr Val Arg Leu His Ala Lys Lys
130 135 140
Ala Asp Thr Ala Ser Tyr His Val Glu Ser Ile Glu Ile Glu Pro Asn
145 150 155 160
Gly Cys Asn His Ala Trp Ile Gln Arg Val His His Leu Val Asn Ile
165 170 175
Glu Met Leu His Cys Ser Gln Glu Val Ala Tyr Met Leu Lys Pro Thr
180 185 190
Tyr Glu Thr Ile Val His Ala Glu Arg Ala Ser Thr Ser Asp Thr Phe
195 200 205
Asn Gly Arg Thr Ile Glu Leu Ser Arg Gly His Arg Val Gln Leu Gly
210 215 220
Asp Ala Ala Tyr Glu Lys Leu Lys Asn Gly Leu Val Gln Thr Arg Ile
225 230 235 240
Glu Gly Lys Lys Pro Gln Leu Val Glu Gly Glu Ile Asn Ser Leu Asn
245 250 255
Asp Ile Arg Gln Arg Trp Ile Asn Ser Gly His Asp Arg Arg Glu Ile
260 265 270
Lys Ala Leu Glu Leu Cys Lys Ile Leu Ser Thr Ile Gly Arg Lys Met
275 280 285
Leu Asn Thr Glu Glu Glu Pro Lys Asn Glu Ala Asp Leu Ser Val Lys
290 295 300
Phe Gln Met Glu Leu Asp Glu Ile Phe Arg Pro Gly Asn Asn Glu Arg
305 310 315 320
Thr Asn Ile Phe Gly Gly Gly Asn His Arg Lys Asp Glu Asp Arg Phe
325 330 335
Tyr Val Leu Ile Met Ile Ala Ala Ser Asp Thr Tyr Lys Gly Arg Ile
340 345 350
Trp Trp Ser Asn Pro Tyr Pro Cys Leu Arg Gly Ser Leu Ile Ala Ala
355 360 365
Glu Ala Gln Leu Gly Asp Val Tyr Asn Thr Met Arg Asn Trp Tyr Gln
370 375 380
Trp Ser Val Arg Pro Thr Tyr Thr Pro Tyr Asp Arg Ser Arg Glu Ser
385 390 395 400
Asp Lys Tyr Val Tyr Ser Arg Val Asn Leu Phe Asp Ser Thr Leu Arg
405 410 415
Pro Gly Asp Lys Ile Ile His Trp Glu Tyr Lys Leu Leu Asn Glu Val
420 425 430
Arg Glu Val Cys Ile Lys Asp Gly Asn Glu Cys Asp Leu Phe Pro Glu
435 440 445
Asp Glu Glu Met Thr Thr Lys Phe Asn Glu Glu Lys Tyr Thr Glu Met
450 455 460
Lys Gly Gln Ile Ile Gln Asp Gly Trp Asn His Arg Asp Phe Lys Met
465 470 475 480
His Lys Ile Leu Gln Asp Gly Ala Asn Val Leu Thr Ile Asp Phe Glu
485 490 495
Lys Asp Ala Arg Ile Gly Asn Gly Ser Val Leu Thr Leu Pro Glu Tyr
500 505 510
Tyr Asn Lys Trp Ile Val Ala Pro Met Phe Asn Ala Lys Leu Arg Ile
515 520 525
Ser Glu Thr Val Ile Ala Lys Ala His Ser Asp Asp Pro Ala Val Ala
530 535 540
Arg Thr Ala Lys Ser Phe Asp His Asp Pro Phe Asn Leu Gln Arg Tyr
545 550 555 560
Cys Leu Ala Arg Tyr Tyr Asp Val Arg Pro Gly Leu Met Gly Arg Ala
565 570 575
Leu Ser Lys Glu Gln Glu Gly Ser Ala Ile Ala Asn Lys Val Ala Ala
580 585 590
Leu Glu Asp Tyr Arg Asp Val Ile Ser Arg Arg Leu Gly Tyr Val Glu
595 600 605
Arg Glu Lys Arg Cys Leu Thr Glu Thr Ala Gln Tyr Ile Leu Glu Lys
610 615 620
Thr Ser Leu Tyr Leu Leu Asn Val Leu Ser Met His Val Lys Pro Thr
625 630 635 640
Ile Asp Met Asp Ile Lys Phe Lys His Pro Ser Ile Asp Thr His Leu
645 650 655
Asp Ile Asp Thr Trp Lys Ile Ala Asp Ile Ser Gln Leu Ile Val Leu
660 665 670
Leu Phe Asp Tyr Leu Phe Glu Ser Arg Lys Glu Ile Arg Ser Val Thr
675 680 685
Glu Ala Arg Trp Ile Leu Phe Lys Val Arg Ser Val Ser Gly Gln Glu
690 695 700
Arg Leu Asn Met Ile Gln Ser Phe Phe Pro Arg Phe Gly Arg Val Val
705 710 715 720
Arg Asp Ile Ala Lys Ala Lys Ile Thr Gln Asp Val Ala His Leu Asn
725 730 735
Phe Phe Pro Leu Phe Phe Ile Ile Gly Asp Asn Met Ala Tyr Ala His
740 745 750
Arg Gln Trp Ser Ile Pro Val Leu Leu Tyr Ala His Asp Ile Arg Ile
755 760 765
Ile Pro Leu Glu Ile Gly Ala Tyr Asn Asn Arg Phe Gly Phe Thr Ser
770 775 780
Tyr Leu Glu Tyr Met Thr Phe Phe Pro Ser His Ala Thr Arg Glu Ala
785 790 795 800
Val Leu Asp Glu Ser Ile Lys Glu Cys Ser Leu Ala Leu Thr Asn Phe
805 810 815
Tyr Leu Asn Thr Asp Ile His Ser Gly Glu Ile Met Arg Asn Val Val
820 825 830
Thr Thr Lys Arg Leu Leu Tyr Glu Thr His Leu Ala Ser Leu Cys Gly
835 840 845
Gly Tyr Ser Asp Gly Leu Leu Trp Tyr Val Pro Ile Thr His Pro Ser
850 855 860
Lys Cys Leu Val Ala Phe Glu Val Ala Asp Asp Val Val Pro Tyr Ser
865 870 875 880
Val Arg Arg Glu Arg Ile Ile Ser Arg Phe Pro Leu Ser Ser Lys His
885 890 895
Leu Arg Gly Ile Ala Leu Ile Ala Val Asn Arg Asn Gln Lys Val Ser
900 905 910
Val Gln Thr Glu Gly Ile Val Thr His Arg Leu Cys Lys Lys Asn Leu
915 920 925
Leu Lys Tyr Val Cys Asp Val Val Leu Phe Lys Phe Ser Gly Tyr Val
930 935 940
Phe Gly Asn Asp Glu Met Leu Thr Lys Leu Leu Asn Val
945 950 955
<210> 184
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV23 CAE53043.1的VP5
<400> 184
Met Gly Lys Val Ile Arg Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ser Ala Ala Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Ser
65 70 75 80
Gly Glu Glu Ile Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Ile Gln
85 90 95
Ala Lys Leu Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Asp Glu Ile Lys Lys Lys Phe Gly Glu Glu Leu Glu
115 120 125
Gln Val Tyr Asp Phe Met Ser Gly Ala Ala Lys Glu Glu Ala Gly Asp
130 135 140
Glu Lys Gln Phe Asp Ile Leu Asn Lys Ala Val Asn Ser Tyr Asn Lys
145 150 155 160
Ile Leu Ala Glu Glu Asp Leu Gln Met Arg Arg Leu Ala Thr Ala Leu
165 170 175
Gln Lys Glu Val Gly Glu Arg Thr Arg Val Glu Thr Thr Met Val Lys
180 185 190
Glu Tyr Arg Asp Lys Ile Asp Ala Leu Arg Ser Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Thr His
260 265 270
Leu Arg Thr Pro Lys Ile Glu Pro Thr Val Val Ser Thr Ile Leu Glu
275 280 285
His Arg Thr Arg Asp Ile Pro Asp Asn Ala Leu Ala Val Ser Val Leu
290 295 300
Ser Lys Asn Arg Val Ile Gln Glu Asn His Lys Glu Leu Met His Ile
305 310 315 320
Lys Glu Glu Ile Leu Pro Arg Phe Lys Lys Ala Met Asp Glu Glu Lys
325 330 335
Glu Ile Cys Gly Ile Glu Asp Lys Val Ile His Pro Lys Val Met Met
340 345 350
Lys Phe Lys Ile Pro Arg Ala Gln Gln Pro His Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Met Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Ser Ile Asp
385 390 395 400
Leu Val His Tyr Glu Asp Leu Thr Ala His Trp His Ala Leu Gly Ala
405 410 415
Ala Gln Met Ala Val Gly Arg Thr Leu Thr Glu Ala Tyr Lys Glu Phe
420 425 430
Leu Asn Leu Ala Ile Ser Asn Ala Tyr Gly Thr Gln Met His Thr Arg
435 440 445
Arg Leu Ile Arg Ser Lys Thr Val His Pro Ile Tyr Leu Gly Ser Leu
450 455 460
His Tyr Asp Val Ser Phe Ala Asp Leu Arg Gly Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Cys Lys Ile
500 505 510
Leu Gly Asp Arg Leu Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 185
<211> 955
<212> PRT
<213> 人工序列
<220>
<223> BTV24 CAE51111.1的VP2
<400> 185
Met Glu Glu Phe Val Ile Pro Val Tyr Ser Glu Asn Glu Ile Pro Tyr
1 5 10 15
Thr Leu Leu Asn Arg Tyr Leu Leu Ala Ile Arg Thr Asp Val Lys Ile
20 25 30
Asn Asp Val Glu Lys Lys His Asn Val Ile Lys Ile Pro Asp Ser Asp
35 40 45
Met Ile Asp Ile Pro Lys Ile Ser Ile Val Asp Ala Leu Ser Tyr Arg
50 55 60
Pro Thr Arg Asn Asp Gly Ile Val Val Pro Arg Leu Leu Asp Ile Thr
65 70 75 80
Leu Arg Ala Tyr Asp Asp Arg Lys Ala Thr Lys Asn Ala Lys Gly Val
85 90 95
Glu Phe Met Thr Asn Ala Lys Trp Met Lys Trp Ala Ile Asp Asp Arg
100 105 110
Met Asp Ile Gln Pro Leu Lys Val Thr Leu Asp Asp His Tyr Ser Val
115 120 125
Asn His Gln Leu Phe Asn Cys Val Val Lys Ala Lys Pro Ala Asn Ala
130 135 140
Asp Thr Val Tyr Tyr Asp Tyr Tyr Pro Leu Glu Ser Lys Met Lys Lys
145 150 155 160
Cys Asn His Thr Asn Leu Asp Met Leu Arg Ser Leu Thr Thr Met Glu
165 170 175
Met Tyr His Ile Leu Gln Gly Ala Ala Tyr Gly Leu Lys Ser Thr Tyr
180 185 190
Glu Leu Lys Ala His Ala Glu Arg Glu Asn Thr Gly Glu Ser Tyr Pro
195 200 205
Ile Gly Glu Arg Leu Leu Gln Ile Pro Arg Gly Thr Lys Ile Gly Ser
210 215 220
Arg Gly Glu Pro Tyr Asn Lys Phe Val Ala Gly Leu Val Lys Val Thr
225 230 235 240
Ile Lys Gly Lys Val Pro Ala Glu Ile Gly Glu Glu Met Asn Gln Leu
245 250 255
Asn Arg Ile Lys Glu Glu Trp Lys Ser Ala Ser His Asp Arg Ser Arg
260 265 270
Ile Arg Ala Leu Glu Leu Cys Lys Ile Leu Ser Thr Ile Gly Arg Lys
275 280 285
Met Leu Asp Ala Lys Glu Glu Pro Lys Asp Glu Met Asp Leu Ser Thr
290 295 300
Arg Phe Gln Phe Lys Leu Asp Glu Lys Phe Thr Lys Thr Asp Ser Glu
305 310 315 320
His Ile Asn Ile Phe Arg Ala Gly Gly Pro Ala Thr Asp Asp Gly Arg
325 330 335
Phe Tyr Ala Leu Ile Ala Ile Ala Ala Thr Asp Thr Gln Lys Gly Arg
340 345 350
Val Trp Arg Met Asn Pro Tyr Pro Cys Leu Arg Gly Ala Ile Ile Ala
355 360 365
Ala Glu Cys Glu Leu Gly Asp Val Tyr Phe Thr Leu Arg Gln Val Tyr
370 375 380
Ser Trp Ser Leu Arg Pro Glu Tyr Gly Gln Lys Glu Lys Pro Leu Glu
385 390 395 400
Val Asn Lys Tyr Val Phe Asn Arg Ile Asn Leu Phe Asp Ser Asp Leu
405 410 415
Thr Val Gly Asp Gln Ile Val His Trp Arg Tyr Glu Leu Tyr Pro Pro
420 425 430
Val Glu Thr Thr Tyr Asp Asp Gly Tyr Leu Cys Gly Ser Asp Lys Glu
435 440 445
Asp Asp Glu Leu Leu Cys Glu Val Asp Glu Glu Lys Tyr Lys Lys Met
450 455 460
Phe Glu Asn Met Ile Glu Gly Gly Trp Asp Gln Glu Arg Phe Lys Leu
465 470 475 480
His Ser Ile Leu Thr Asp Pro Asn Leu Leu Thr Ile Asp Phe Glu Lys
485 490 495
Asp Ala Tyr Leu Asn Ser Arg Ser Glu Leu Val Phe Pro Glu Tyr Phe
500 505 510
Asp Lys Trp Ile Asn Ser Pro Met Phe Asn Ala Lys Leu Arg Leu Ala
515 520 525
His Gly Glu Ile Ala Thr Arg Arg Ala Asn Asp Pro Trp Asn Asn Arg
530 535 540
Ala Val His Gly Tyr Ile Arg Thr Ser Thr Glu Ser Leu Gly Tyr Ala
545 550 555 560
Leu Gly Ser Tyr Tyr Asp Leu Arg Leu Gln Leu Phe Gly Asp Ala Leu
565 570 575
Ser Leu Glu Gln Arg Gln Ser Ala Val Phe Glu His Leu Asn Lys Gln
580 585 590
Asp Asp Phe Phe Thr Leu Thr Gly Tyr Ala Lys Asp Gln Val Val Cys
595 600 605
Pro His Ser Gly Gly Ala Phe Tyr Thr Phe Arg Arg Val Ala Leu Glu
610 615 620
Ile Leu Ser Asn Tyr Glu Lys Leu Thr Pro Asp Leu His Glu Gly Leu
625 630 635 640
Glu His Gln Thr Tyr Thr His Pro Lys Ile Asn Glu Tyr Phe Asn Arg
645 650 655
Phe Val Leu Asp Met Lys Asp Phe Ser Gln Leu Ile Cys Phe Val Phe
660 665 670
Asp His Phe Phe Glu Arg His Glu Gln Leu Arg Asp Val Ala Glu Ala
675 680 685
Arg Arg Leu Val Tyr Leu Ile Gln Lys Ser Asp Gly Pro Glu Arg Leu
690 695 700
Asp Val Leu Gln Arg Asn Phe Pro Asn Phe Phe Arg Arg Phe Met Asn
705 710 715 720
Leu Lys Asp Val Lys Tyr Ile Tyr Asp Leu Asn Val Val Asn Phe Leu
725 730 735
Pro Leu Leu Phe Leu Ile Gln Asp Asn Ile Ser Tyr Trp His Arg Gln
740 745 750
Trp Ser Val Pro Val Ile Leu Tyr Gly Asp Ile Ile Arg Leu Ile Pro
755 760 765
Val Glu Val Gly Ala Tyr Ala Asn Arg Phe Gly Met Lys Ser Phe Phe
770 775 780
Asn Phe Thr Arg Phe His Pro Gly Asp Ser Lys Lys Lys Gln Asn Ala
785 790 795 800
Asp Asp Thr His Lys Glu Phe Gly Leu Ile Cys Phe Glu Tyr Tyr Thr
805 810 815
Lys Thr Lys Ile Ser Gln Gly Gly Val Asp Ile Ser Val Val Thr Thr
820 825 830
Lys Met Asp Thr Leu Lys Val His Leu Ala Ser Leu Cys Ser Gly Ile
835 840 845
Ala Asp Ser Ile Val Tyr Thr Leu Pro Val Ala His Pro Lys Lys Cys
850 855 860
Val Val Leu Ile Val Ile Gly Asp Asp Asn Val Asp Met Gln Ile Arg
865 870 875 880
Ser Glu Gln Ala Ile Ser Arg Tyr Tyr Tyr Ser Arg Arg His Ile Ala
885 890 895
Gly Val Val Ser Ile Cys Ile Ser Gln Asp Gly Gly Leu Lys Ala Tyr
900 905 910
Ser Thr Gly Ile Val Lys His Arg Ile Cys Glu Lys Ser Ile Leu Lys
915 920 925
Tyr Lys Cys Lys Val Val Leu Val Arg Met Pro Gly Tyr Val Phe Gly
930 935 940
Asn Asp Glu Leu Met Thr Lys Leu Leu Asn Val
945 950 955
<210> 186
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV24 CAE53046.1的VP5
<400> 186
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Asn Ala Leu Ser Ser Asn Thr Ala Lys Lys Ile Tyr Ser Thr Ile Gly
20 25 30
Lys Ala Ala Glu Arg Phe Ala Glu Ser Glu Ile Gly Ala Ala Thr Ile
35 40 45
Asp Gly Leu Val Gln Gly Ser Val His Ser Ile Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Thr
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Met Gln
85 90 95
Thr Lys Ile Lys Glu Leu Glu Asp Glu Gln Arg Asn Glu Leu Val Arg
100 105 110
Leu Lys Tyr Asn Lys Glu Ile Thr Lys Glu Phe Gly Lys Glu Leu Glu
115 120 125
Glu Val Tyr Asp Phe Met Asn Gly Glu Ala Lys Glu Glu Glu Val Val
130 135 140
Gln Glu Gln Tyr Ser Met Leu Cys Lys Ala Val Asp Ser Tyr Glu Lys
145 150 155 160
Ile Leu Lys Ala Glu Asp Ser Lys Met Ala Ile Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Ser Glu Arg Ser Gln Asp Glu Ile Lys Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Lys Asn Ala Ile Glu Ile Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Ala Ser Glu Glu Val Pro Leu Ile Gly Ala
225 230 235 240
Gly Met Ala Thr Ala Val Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Met Arg Ser Pro Lys Ile Glu Pro Thr Ile Ile Ala Thr Thr Leu Glu
275 280 285
His Arg Phe Lys Glu Ile Pro Asp Glu Gln Leu Ala Val Ser Val Leu
290 295 300
Asn Lys Lys Thr Ala Val Thr Asp Asn Cys Asn Glu Ile Ala His Ile
305 310 315 320
Lys Gln Glu Ile Leu Pro Lys Phe Lys Gln Ile Met Asp Glu Glu Arg
325 330 335
Glu Ile Glu Gly Ile Glu Asp Lys Val Ile His Pro Arg Val Met Met
340 345 350
Arg Phe Lys Ile Pro Arg Thr Gln Gln Pro Gln Ile His Ile Tyr Ala
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Arg Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Thr Ser His Trp His Ala Leu Gly Leu
405 410 415
Ala Gln Glu Ala Ser Gly Arg Thr Leu Thr Glu Ala Tyr Arg Glu Phe
420 425 430
Leu Asn Leu Ser Ile Ser Asn Thr Tyr Ser Ser Ala Ile His Ala Arg
435 440 445
Arg Met Ile Arg Ser Arg Ala Val His Pro Ile Phe Leu Gly Ser Met
450 455 460
His Tyr Asp Ile Thr Tyr Glu Ala Leu Lys Asn Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Glu Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Ile
500 505 510
Leu Gly Asp Lys Ile Asp Val Pro Leu Phe Leu Arg Asn Ala
515 520 525
<210> 187
<211> 959
<212> PRT
<213> 人工序列
<220>
<223> BTV25 ACJ06702.1的VP2
<400> 187
Met Asp Glu Leu Val Ile Pro Ile Phe Ser Ala Asp Glu Met Pro Tyr
1 5 10 15
Ala Leu Ile Ser His Tyr Pro Leu Ala Ile Gln Thr Asn Arg Lys Ile
20 25 30
Gln Asn Asp Arg Glu Phe His Asp Leu Thr Lys Ile Pro Gln Ser Asn
35 40 45
Met Ile Asp Ile Ser Gln His Gly Val Ile Arg Ala Leu Asp Tyr Arg
50 55 60
Pro Ser Arg Asn Asp Gly Ile Val Ile Pro Arg Leu Leu Asp Ile Thr
65 70 75 80
Leu His Ala Tyr Asp Lys Arg Arg Ser Met Lys Asp Ile Cys Gly Leu
85 90 95
Glu Phe Lys Thr Asp Val Lys Trp Met Gln Trp Ala Ile Asn Asp Arg
100 105 110
Met Asp Ile Gln Pro Leu Lys Val Glu Ile Asp Asn Glu His Thr Val
115 120 125
Asn His Gln Leu Phe Asn Cys Asn Ile Lys Ile Arg Ser Lys Asn Val
130 135 140
Asp Thr Val Tyr Tyr Asp Tyr His Pro Leu Glu Ser Glu Ala Thr Lys
145 150 155 160
Cys Asn His Ala Asn Ile Glu Leu Met Arg Ser Leu Thr Met Val Glu
165 170 175
Leu Tyr His Val Leu Gln Gly Ala Ala Tyr Ala Leu Lys Thr Asn Phe
180 185 190
Glu Leu Ile Ala Thr Ala Glu Arg Gly Asp Val Arg Gln Arg Tyr Asp
195 200 205
Ala Ala Gly Val Arg Trp Ser Arg Leu Ser Arg Asp Gln Arg Ile Gly
210 215 220
Tyr Ser Gly Asn Ala Tyr Asp Met Phe Met Ala Asn Leu Val Gln Val
225 230 235 240
Glu Val Lys Gly Lys Cys Ala Asp Glu Ile Ala Gly Glu Met Gly Arg
245 250 255
Leu Ala Lys Ile Lys Asp Lys Trp Val Ser Asn Gly Phe Ser Gly Thr
260 265 270
Val Ile Phe Val Ser Glu Leu Cys Thr Glu Phe Ser Ala Ile Gly Arg
275 280 285
Lys Met Met Asp Thr His Glu Glu Pro Lys Asp Glu Glu Gly Ile Ser
290 295 300
Arg Arg Phe Gln Ile Glu Leu Asp Gly Met Phe Ser Ala Ala Arg Gln
305 310 315 320
Glu Asn Lys Asn Val Leu Ser Gln Arg Gly Ser Lys Val Thr Asp Glu
325 330 335
Ser Lys Phe Tyr Ala Leu Ile Met Ile Ala Ala Ser Asp Thr Gln Gln
340 345 350
Gly Arg Val Trp Arg Thr Asn Leu Tyr Pro Cys Leu Arg Gly Ala Leu
355 360 365
Val Ala Ala Glu Cys Val Met Gly Asp Val Tyr His Thr Leu Arg Thr
370 375 380
Val Phe Asn Trp Ser Val Arg Gly Thr Tyr Gly Gly Thr Glu Arg Asn
385 390 395 400
Leu Glu Asn Asn Arg Tyr Ile Phe Ser Arg Ile Asn Leu Phe Glu Thr
405 410 415
Asn Leu Pro Ser Gly Ser Lys Ile Val His Trp Cys Tyr Glu Glu Val
420 425 430
Ala Pro Ile Glu Thr Thr Tyr Asp Asn Gly Tyr Ile Cys Lys Ser Gln
435 440 445
Glu Gly Val Gln Asn Asp Glu Lys Leu Cys Lys Ile Asp Asp Asp Leu
450 455 460
Tyr Ala Arg Met Arg Asp Glu Ala Ile Thr Gly Gly Trp Arg Gln Glu
465 470 475 480
Asp Phe Lys Leu His Lys Ile Leu Thr Asn Pro Asn Leu Leu Thr Ile
485 490 495
Asp Phe Glu Lys Glu Ala Tyr Ile Thr Ser Thr Ser Asp Leu Ile Leu
500 505 510
Pro Asp Tyr Phe Asp Lys Trp Ile Lys Ala Pro Ile Phe Asn Ala Arg
515 520 525
Leu Arg Ile Thr Asn Gly Val Ile Glu Ser Glu Lys Lys Ser Asp Pro
530 535 540
Trp Asn Lys Arg Val Ala Asn Gly Thr Met Lys Thr Ser Ala Glu Ser
545 550 555 560
Leu Gly Tyr Val Leu Gly Arg Phe Tyr Asp Leu Arg Met Gln Phe Val
565 570 575
Glu Asp Val Leu Asn Thr Arg Gln Gln Lys Ser Leu Ala Leu Gln His
580 585 590
Ile Arg Asn Leu Glu Asp Phe Thr Arg Leu Thr Asp Tyr Gly Glu Glu
595 600 605
Gln Asn Ile Cys Pro His Ala Gly Gly Ala Met Tyr Thr Phe Arg Arg
610 615 620
Val Ala Leu Glu Ile Ile Thr Asn Tyr Glu Arg Leu Asn Pro Met Asp
625 630 635 640
His Glu Gly Asn Glu Tyr Gln Thr Tyr Arg His Pro Ala Ile Asp Tyr
645 650 655
Ser Lys Leu Gly Asp Val Leu Lys Met Lys Asp Leu Ser Gln Leu Ile
660 665 670
Cys Phe Val Phe Asp Ser Ile Phe Glu Arg Arg Asp Gln Leu Arg Glu
675 680 685
Val Gly Glu Ala Arg Met Ile Ile Tyr Gln Ile Gln Asn Ala Thr Gly
690 695 700
Glu Arg Arg Leu Asp Val Leu Lys Arg Glu Phe Pro Thr Phe Tyr Val
705 710 715 720
Trp Phe His Arg Leu Arg Asp Val Glu Leu Ile Arg Asp Leu Asn Val
725 730 735
Ile Asn Phe Leu Pro Leu Leu Phe Leu Val Arg Asp Asn Ala Ala Tyr
740 745 750
Arg His Arg Gln Trp Ser Ile Pro Met Ile Leu Tyr Asp Glu Glu Ile
755 760 765
Lys Leu Ile Pro Val Glu Val Gly Ala Tyr Ala Asn Arg Phe Gly Phe
770 775 780
Lys Ser Phe Ile Asn Phe Thr Arg Phe His Pro Gly Asp Ala Arg Lys
785 790 795 800
Arg Gln Glu Val Ser Ala Phe His Lys Ala Tyr Gly Ser Ile Cys Tyr
805 810 815
Arg Tyr Tyr Thr Asp Thr Thr Ile Ser Gln Gly Glu Val Asn Val Pro
820 825 830
Val Ile Thr Thr Lys Leu Asp Thr Leu Arg Val His Leu Ala Ala Leu
835 840 845
Cys Gly Gly Leu Ser Asp Ser Leu Val Tyr Thr Leu Pro Ile Thr His
850 855 860
Pro Glu Lys Cys Leu Val Leu Val Ile Val Gly Asp Asn Lys Leu Asn
865 870 875 880
Pro Gln Ile Arg Ser Glu Leu Val Ile Ser Arg Tyr Arg Tyr Ser Arg
885 890 895
Arg His Ile Arg Gly Val Val Ser Val Cys Ile Asp Gln Glu Gly Leu
900 905 910
Pro Asn Val Tyr Ser Thr Gly Ile Ala Arg His Lys Ile Tyr Glu Lys
915 920 925
Thr Val Leu Arg Tyr Arg Cys Lys Ile Ile Leu Val Lys Ile Ser Gly
930 935 940
Tyr Val Phe Gly Asn Asp Glu Leu Met Thr Lys Leu Leu Asn Val
945 950 955
<210> 188
<211> 526
<212> PRT
<213> 人工序列
<220>
<223> BTV25 ACJ06704.1的VP5
<400> 188
Met Gly Lys Ile Ile Arg Ser Leu Ser Arg Phe Gly Lys Lys Val Gly
1 5 10 15
Ser Ala Leu Thr Ser Asn Thr Ala Lys Lys Ile Tyr Ala Thr Ile Gly
20 25 30
Arg Ala Ala Glu Arg Leu Ala Glu Ser Glu Ile Gly Ser Ala Thr Ile
35 40 45
Asp Gly Leu Leu Gln Gly Ser Val His Ser Leu Val Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Thr
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Leu Gln
85 90 95
Val Lys Leu Lys Glu Leu Glu Asp Glu Gln Arg Asn Asp Leu Val Arg
100 105 110
Leu Arg Tyr Gly Glu Gln Ile Arg Ala Lys Phe Gly Ala Glu Leu Asp
115 120 125
Glu Ile Tyr Gln Phe Met Thr Ser Ala Ile Arg Asn Asp Gly Glu Ala
130 135 140
Lys Glu Glu Tyr Glu Ile Leu Asn Lys Ala Ile Asn Ser Tyr Glu Lys
145 150 155 160
Ile Leu Lys Ala Glu Glu Thr Gln Ile Ser Thr Leu Ala Arg Ala Leu
165 170 175
Gln Arg Glu Ala Ser Glu Arg Ser Glu Asn Glu Ile Thr Met Val Lys
180 185 190
Glu Tyr Arg Gln Lys Ile Asp Ala Leu Arg Asp Ala Ile Glu Val Glu
195 200 205
Arg Asp Gly Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr
210 215 220
Ala Asp Val Leu Glu Ala Thr Ser Glu Glu Val Pro Leu Val Gly Ala
225 230 235 240
Gly Val Ala Ala Thr Ile Ala Thr Gly Arg Ala Ile Glu Gly Ala Tyr
245 250 255
Lys Leu Lys Lys Val Ile Ser Ala Leu Ser Gly Ile Asp Leu Ser His
260 265 270
Met Arg Thr Pro Arg Val Glu Pro Thr Thr Leu Ala Thr Thr Leu Asp
275 280 285
Arg Arg Phe Gly Glu Ile Ser Asp Lys Asp Leu Ala Ile Gly Val Leu
290 295 300
Asn Lys Arg Thr Ser Val Leu Asp Asn Tyr Ser Glu Ile Ala His Ile
305 310 315 320
Lys Gln Glu Ile Met Pro Arg Phe Lys Arg Ile Leu Asp Glu Glu Arg
325 330 335
Lys Ile Glu Gly Ala Asn Lys Lys Tyr Ile His Pro Arg Val Met Met
340 345 350
Lys Phe Lys Ile Pro Arg Thr Gln Gln Pro His Ile His Ile Tyr Ser
355 360 365
Ala Pro Trp Asp Ser Asp Asp Val Phe Phe Phe His Cys Val Ser His
370 375 380
His His Arg Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp
385 390 395 400
Val Val His Phe Glu Asp Leu Ala Ser His Trp His Ala Leu Gly Ser
405 410 415
Ala Gln Glu Ala Ser Gly Arg Thr Leu Thr Glu Ala Tyr Lys Glu Phe
420 425 430
Leu Asn Leu Ser Ile Ser Ser Ala Phe Ser Ser Pro Ile His Ala Lys
435 440 445
Arg Met Ile Arg Ser Arg Thr Val His Pro Ile Tyr Leu Gly Ser Met
450 455 460
His Tyr Asp Val Thr Tyr Glu Val Leu Lys Asn Asn Ala Gln Arg Ile
465 470 475 480
Val Tyr Asp Asp Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His
485 490 495
Phe Gln Arg Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Ile
500 505 510
Leu Gly Glu Lys Ile Asp Ile Ser Leu Phe Leu Arg Asn Ala
515 520 525
<210> 189
<211> 957
<212> PRT
<213> 人工序列
<220>
<223> BTV26 AED99447.1的VP2
<400> 189
Met Glu Glu Phe Val Ile Pro Ile Tyr Ser Ser Arg Asp Met Pro Leu
1 5 10 15
Ala Leu Ile Ser Gln Tyr Pro Leu Ala Val Gln Val Asp Ala Ile Met
20 25 30
Ala Asp Ile Gly Asp Asp His Asn Leu Ala Leu Ile Pro Glu Leu Asn
35 40 45
Met Ile Asp Ile Ser Lys Gln Gly Ile Ile Asp Ala Leu Asp Tyr Arg
50 55 60
Pro Ala Arg Asn Asp Gly Val Val Val Pro Arg Leu Leu Asp Ile Thr
65 70 75 80
Leu His Ala Tyr Asp Cys Lys Arg Ser Met Arg Thr Ala Arg Asn Thr
85 90 95
Glu Phe Ala Thr Asp Thr Glu Trp Met Lys Trp Ala Ile Glu Asp Arg
100 105 110
Met Asp Val Gln Pro Leu Lys Ile Leu Ile Asp Asp His Tyr Phe Ile
115 120 125
Asn His Gln Leu Phe Asn Cys Asn Val Lys Ala Lys Phe Gly Asn Val
130 135 140
Asp Thr Ile Tyr Tyr Asp Leu His Pro Leu Glu Lys Lys Glu Lys Lys
145 150 155 160
Cys Asn His Met Asn Ile Glu Leu Met Arg Ser Leu Thr Met Thr Glu
165 170 175
Met Phe His Val Leu Gln Gly Ala Ala Tyr Thr Leu Lys Thr Asn Tyr
180 185 190
Thr Leu Thr Ala Thr Ala Glu Arg Gln Asp Ile Ser His Ser Phe Glu
195 200 205
Asn Val Gly Leu Arg Trp Gln Asn Leu Ala Pro Asn Thr Arg Ile Glu
210 215 220
Asn Arg Gly Ser Ala Tyr Asp Lys Phe Ile Ala Gly Leu Val Gln Val
225 230 235 240
Arg Ile Asp Gly Lys Cys Pro Asn Glu Ile Thr Ala Glu Leu Asn Gln
245 250 255
Leu Arg Ser Val Lys Glu Lys Trp Val Gly Ala Gln Tyr Gln Gly Asn
260 265 270
Ala Ile Tyr Ala His Glu Ile Cys Ser Val Met Ser Ala Ile Gly Arg
275 280 285
Lys Met Leu Asn Ala Arg Glu Glu Pro Val Asp Glu Glu Gly Ile Ser
290 295 300
Arg Arg Phe Gln Gln Glu Ile Asp Asp Val Phe Ser Gln Met Asn Arg
305 310 315 320
Glu Asn Val Ala Val Arg Arg Val Ala Val His Ala Asn Asp Glu Gln
325 330 335
Lys Phe Phe Ala Leu Met Val Ile Ala Ala Thr Asp Val Asn Gln Gly
340 345 350
Arg Ile Trp Arg Thr Asn Pro Tyr Pro Cys Leu Arg Gly Thr Leu Ile
355 360 365
Ala Ala Glu Cys Ala Leu Gly Asp Val Tyr Ser Ala Met Arg Arg Ile
370 375 380
Phe Ser Trp Ser Val Arg Pro Thr Tyr Gly Thr Gly Glu Lys Gln Leu
385 390 395 400
Glu Asn Asn Arg Tyr Val Tyr Ser Arg Ile Asn Leu Phe Glu Ser Asn
405 410 415
Ile Ser Val Asp Glu Glu Val Ile His Trp Arg Tyr Glu Leu Leu Ala
420 425 430
Pro Ala Glu Thr Thr Tyr Asp Arg Gly Tyr Ile Cys Ser Val Glu Arg
435 440 445
Gly Asp Asp Asp Leu Leu Cys Lys Ile Asp Asp Ala Ala Tyr Lys Leu
450 455 460
Met Phe Asp Glu Ala Val Arg Val Gly Trp Gln Gln His Thr Phe Lys
465 470 475 480
Leu Tyr Lys Met Leu Thr Glu Pro Asn Leu Leu Thr Ile Asp Phe Glu
485 490 495
Lys Asp Ala Tyr Leu Gly Pro Asp Ser Ser Leu Val Met Pro Asp Tyr
500 505 510
Tyr Asn Lys Trp Ile Arg Ser Pro Met Phe Asn Ala Arg Leu Arg Leu
515 520 525
Thr Lys Ala Arg Ile Asn Ala Glu Lys Thr Gly Asp Pro Trp Asn Asp
530 535 540
Arg Val Val Gly Gly Thr Ile Lys Thr Pro Val Glu Ser Leu Gly Tyr
545 550 555 560
Thr Leu Gly Arg Phe Tyr Asp Leu Arg Leu Gln Phe Val Glu Asp Val
565 570 575
Leu Ser Tyr Arg Gln Gln Gln Ser Ala Ile Phe Gln Tyr Met Gly Ala
580 585 590
Arg Glu Asp Phe Ala Met Leu Thr His Tyr Ala Arg Gly Glu Arg Asp
595 600 605
Ile Cys Pro His Ala Gly Gly Thr Leu Tyr Ile Thr Arg Arg Val Ala
610 615 620
Leu Glu Val Leu Thr Ser Tyr Glu Arg Leu Asp Pro Gln Tyr His His
625 630 635 640
Gly Arg Glu His Leu Thr Tyr Cys His Pro Ser Ile Asp Tyr Thr Arg
645 650 655
Arg Glu Arg Ile Phe Glu Ile Glu Asp Leu Ala Gln Leu Ile Cys Tyr
660 665 670
Val Ile Asp Tyr Ile Phe Glu Lys Arg Asp Ser Leu Arg Glu Val Gly
675 680 685
Glu Ala Arg Arg Ile Val Tyr Leu Ile Gln Ser Val Thr Gly Ile Arg
690 695 700
Arg His Glu Val Leu Asn Gln Ala Phe Pro Arg Phe Phe Ala Trp Phe
705 710 715 720
Leu Arg Leu Lys Asn Val Glu Val Ile Arg Asp Leu Asn Ile Val Asn
725 730 735
Phe Leu Pro Leu Leu Phe Leu Val Arg Asp Asn Ile Ser Tyr Gln His
740 745 750
Arg Gln Trp Ser Val Pro Met Ile Leu Tyr Asp Gln Thr Ile Lys Leu
755 760 765
Ile Pro Val Glu Val Gly Ala Tyr Ala Asn Arg Val Gly Phe Lys Ser
770 775 780
Phe Phe Asn Phe Val Gln Phe His Pro Gly Asp Ala Gln Lys Arg Gln
785 790 795 800
Glu Ala Asp Glu Ser His Lys Lys Met Gly Gln Ile Cys Tyr Ala Tyr
805 810 815
Tyr Thr Asp Thr Thr Ile Ser Gln Gly Lys Val Asp Thr Pro Val Ile
820 825 830
Ala Thr Lys Met Asp Thr Leu Arg Val His Leu Ala Ala Val Cys Ala
835 840 845
Gly Leu Ser Asp Ser Leu Val Tyr Ala Leu Pro Val Ala His Pro Glu
850 855 860
Arg Cys Ile Val Leu Ile Ile Ile Gly Asp Glu Lys Leu Asp Pro Asn
865 870 875 880
Val Arg Thr Glu Leu Val Val Ser Arg Tyr Arg Tyr Ser Arg Lys His
885 890 895
Ile Arg Gly Ala Val Ser Ile Cys Met Tyr Lys Asn Gly Gln Phe Arg
900 905 910
Val His Thr Thr Gly Ile Val Arg His Arg Ile Cys Glu Lys Thr Ile
915 920 925
Leu Asn Phe Arg Cys Arg Ala Ile Leu Val Arg Thr Pro Gly Tyr Val
930 935 940
Phe Gly Asn Asp Glu Leu Met Thr Lys Leu Leu Asn Val
945 950 955
<210> 190
<211> 523
<212> PRT
<213> 人工序列
<220>
<223> BTV26 AER60536.1的VP5
<400> 190
Met Gly Lys Ile Ile Lys Ser Leu Ser Arg Phe Gly Lys Lys Ile Gly
1 5 10 15
Ser Ala Leu Thr Ser Asn Ala Ala Lys Lys Ile Tyr Ala Thr Val Gly
20 25 30
Lys Ala Ala Glu Arg Leu Ala Thr Ser Glu Leu Gly Thr Ala Ala Ile
35 40 45
Asp Gly Leu Leu Gln Gly Ser Val His Ser Met Ile Thr Gly Glu Ser
50 55 60
Tyr Gly Glu Ser Val Lys Gln Ala Val Leu Leu Asn Val Leu Gly Thr
65 70 75 80
Gly Glu Glu Leu Pro Asp Pro Leu Ser Pro Gly Glu Arg Gly Met Gln
85 90 95
Met Lys Ile Gln Glu Leu Glu Asp Glu Gln Arg Ser Glu Leu Ile Arg
100 105 110
Leu Lys Tyr Asp Asp Glu Ile Thr Arg Ala Phe Gly Asn Glu Leu Arg
115 120 125
Glu Val Tyr Gln Phe Met Asn Gly Gln Val Arg Ala Asp Leu Asp Gln
130 135 140
Tyr Asp Val Leu Arg Lys Ala Val Asp Ser Tyr Glu Lys Leu Leu Asp
145 150 155 160
Ala Glu Asp Ala Lys Met Ala Ile Leu Ala Arg Ala Leu Gln Lys Glu
165 170 175
Met Arg Glu Arg Ser Ala Glu Glu Ala Lys Met Val Arg Glu Tyr Arg
180 185 190
Ser Lys Ile Asp Ala Leu Lys Glu Ala Ile Glu Val Glu Arg Asp Gly
195 200 205
Met Gln Glu Glu Ala Ile Gln Glu Ile Ala Gly Met Thr Ala Asp Val
210 215 220
Leu Glu Ala Thr Ala Glu Glu Val Pro Leu Ile Gly Ala Gly Ala Ala
225 230 235 240
Ala Ala Ile Ala Ser Gly Arg Ala Ile Glu Gly Ala Tyr Lys Leu Lys
245 250 255
Lys Val Ile Asn Ala Leu Ser Gly Ile Asp Leu Ser His Met Arg Thr
260 265 270
Pro Lys Ile Glu Pro Thr Val Ile Ser Thr Val Leu Glu His Arg Phe
275 280 285
Lys Ser Ile Pro Asp Asn Leu Leu Ala Asn Gly Val Leu Ser Lys Lys
290 295 300
Ile Ser Ile Met Asp Asn Gln Asn Glu Ile Arg His Ile Lys Arg Glu
305 310 315 320
Ile Leu Pro Arg Phe Lys Arg Ile Met Asp Glu Glu Arg Ala Ala Ala
325 330 335
Asn Arg Glu His Lys Val Ile His Pro Thr Val Met Met Lys Phe Lys
340 345 350
Val Pro Arg Ala Gln Gln Pro Gln Ile His Ile Tyr Ala Ala Pro Trp
355 360 365
Asp Ser Asp Glu Val Phe Phe Phe His Cys Val Ser His His His Arg
370 375 380
Asn Glu Ser Phe Phe Leu Gly Phe Asp Leu Gly Ile Asp Ile Val His
385 390 395 400
Phe Glu Asp Leu Thr Asn His Trp His Ala Leu Gly Val Ala Gln Glu
405 410 415
Ala Lys Gly Arg Thr Leu Gly Glu Ala Tyr Lys Glu Phe Leu Asn Leu
420 425 430
Pro Ile Ser Ser Ser Phe Ser Ser Arg Ile His Ala Arg Arg Met Val
435 440 445
Arg Ser Arg Leu Val His Pro Ile Phe Leu Gly Ser Met His Tyr Asp
450 455 460
Val Thr Tyr Glu Thr Leu Lys Asn Asn Ala Gln Arg Ile Val Tyr Asp
465 470 475 480
Glu Glu Leu Gln Met His Ile Leu Arg Gly Pro Leu His Phe Gln Arg
485 490 495
Arg Ala Ile Leu Gly Ala Leu Lys Phe Gly Val Lys Ile Leu Gly Asp
500 505 510
Lys Ile Asp Ile Pro Leu Phe Leu Arg Asn Ala
515 520
<210> 191
<211> 17
<212> DNA
<213> 人工序列
<220>
<223> VP2 BTV1 5' UTR区
<400> 191
gttaaaatag tagcgcg 17
<210> 192
<211> 37
<212> DNA
<213> 人工序列
<220>
<223> VP2 BTV1 3' UTR区
<400> 192
tctgcttgac cgcagatccg cgcactatta gacttac 37
<210> 193
<211> 25
<212> DNA
<213> 人工序列
<220>
<223> VP5 BTV1 5' UTR区
<400> 193
gttaaaaagt gcgcccttag cgaag 25
<210> 194
<211> 29
<212> DNA
<213> 人工序列
<220>
<223> VP5 BTV1 3' UTR区
<400> 194
acgcagcgac gggaagcact tacacttac 29

Claims (7)

1.包含重组BTV载体的组合物，所述载体是来源于BTV-1基因组的载体，其中BTV-1VP2的编码序列被替换为BTV-8VP2的编码序列。
2.重组BTV载体，所述载体是来源于BTV-1基因组的载体，其中BTV-1VP2的编码序列被替换为BTV-8VP2的编码序列。
3.一种产生重组BTV载体的方法，其包括用如下RNA转染细胞：
(a)编码BTV-8的VP2的RNA；和
(b)包含编码BTV-1的VP1、VP3、VP4、VP5、VP7、NS1、NS2、VP6NS4和NS3/3A的转录物的RNA。
4.权利要求1的组合物或权利要求2的载体在制备用于接种动物的方法中所用的药剂中的用途，所述方法包括至少一次施用所述药剂。
5.权利要求4的用途，其中所述方法包括初免-加强施用方案。
6.权利要求1的组合物或权利要求2的载体在制备用于诱导动物抗一种或多种病原体的免疫原性或保护性应答的方法中所用的药剂的用途，所述方法包括至少一次施用所述药剂。
7.权利要求6的用途，其中所述动物为牛类动物、绵羊类动物、山羊类动物或马类动物。
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