CN110560249A - Method for quickly separating mosquito pupae - Google Patents
Method for quickly separating mosquito pupae Download PDFInfo
- Publication number
- CN110560249A CN110560249A CN201910703749.5A CN201910703749A CN110560249A CN 110560249 A CN110560249 A CN 110560249A CN 201910703749 A CN201910703749 A CN 201910703749A CN 110560249 A CN110560249 A CN 110560249A
- Authority
- CN
- China
- Prior art keywords
- pupae
- larvae
- mosquito
- water
- mixture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000255925 Diptera Species 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 27
- 241000256113 Culicidae Species 0.000 claims abstract description 20
- 239000005457 ice water Substances 0.000 claims abstract description 19
- 239000000203 mixture Substances 0.000 claims abstract description 18
- 239000001963 growth medium Substances 0.000 claims description 5
- 241000382353 Pupa Species 0.000 claims 7
- 238000001914 filtration Methods 0.000 claims 2
- 239000002609 medium Substances 0.000 claims 2
- 238000000151 deposition Methods 0.000 claims 1
- 238000000926 separation method Methods 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 description 7
- 241000256173 Aedes albopictus Species 0.000 description 4
- 239000011159 matrix material Substances 0.000 description 4
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000005667 attractant Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000005871 repellent Substances 0.000 description 1
- 230000002940 repellent Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/30—Rearing or breeding invertebrates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03B—SEPARATING SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS
- B03B5/00—Washing granular, powdered or lumpy materials; Wet separating
- B03B5/28—Washing granular, powdered or lumpy materials; Wet separating by sink-float separation
- B03B5/30—Washing granular, powdered or lumpy materials; Wet separating by sink-float separation using heavy liquids or suspensions
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
Abstract
本发明公开了一种快速分离蚊虫蛹的方法。它是将蚊子的幼虫和蛹的混合物倒入到冰水中，蛹漂浮于水面，幼虫沉于冰水中，分别捞起上层的蛹和下层的幼虫，得到分离的蛹和幼虫。本发明人发现，当蚊子的幼虫和蛹放于冰水中，幼虫由于被冰冻一瞬间会自动下沉，而蛹则会漂于水面，当捞出蛹后，冰水的温度回升至常温(25℃)的时候，幼虫又会自动上浮上来。因此，本发明能够有效的分离蚊子的幼虫和蛹，提高蚊子幼虫和蛹的分离效率，省时省力，不损伤蛹。
The invention discloses a method for rapidly separating mosquito pupae. It is to pour the mixture of mosquito larvae and pupae into ice water, the pupae float on the water surface, and the larvae sink in the ice water, and the pupae of the upper layer and the larvae of the lower layer are picked up respectively to obtain separated pupae and larvae. The inventor found that when the larvae and pupae of mosquitoes were placed in ice water, the larvae would sink automatically due to being frozen for a moment, while the pupae would float on the water surface. After the pupae were pulled out, the temperature of the iced water rose to normal temperature (25 ℃), the larvae will automatically float up again. Therefore, the present invention can effectively separate mosquito larvae and pupae, improve the separation efficiency of mosquito larvae and pupae, save time and labor, and do not damage pupae.
Description
技术领域technical field
本发明属于蚊虫防治领域，具体涉及一种快速分离蚊虫蛹的方法。The invention belongs to the field of mosquito control, and in particular relates to a method for rapidly separating mosquito pupae.
背景技术：Background technique:
蚊子主要危害是传播疾病。据研究，蚊子传播的疾病达80多种之多。消灭蚊子，是预防减少疾病的有效途径。在实验中，我们需要大量的蚊虫进行引诱剂、趋避剂、不育技术等研究。因此，需要获得大量蚊虫蛹及后期成虫进行实验验证。The main hazard of mosquitoes is the transmission of diseases. According to research, there are more than 80 kinds of diseases transmitted by mosquitoes. Eliminating mosquitoes is an effective way to prevent and reduce diseases. In experiments, we need a large number of mosquitoes to conduct research on attractants, repellents, sterile techniques, etc. Therefore, it is necessary to obtain a large number of mosquito pupae and late adults for experimental verification.
发明内容：Invention content:
本发明的目的是提供一种快速分离蚊虫蛹的方法。The purpose of the present invention is to provide a method for rapidly separating mosquito pupae.
本发明的快速分离蚊虫蛹的方法，包括以下步骤：The method for rapidly separating mosquito pupae of the present invention comprises the following steps:
将蚊子的幼虫和蛹的混合物倒入到冰水中，蛹漂浮于水面，幼虫沉于冰水中，分别捞起上层的蛹和下层的幼虫，得到分离的蛹和幼虫。The mixture of mosquito larvae and pupae is poured into ice water, the pupae float on the water surface, and the larvae sink in the ice water, and the pupae of the upper layer and the larvae of the lower layer are picked up respectively to obtain separated pupae and larvae.
收集的蛹可以用于蛹期试验或培养成虫的实验需要。The collected pupae can be used for pupal stage experiments or the experimental needs of cultivating adults.
优选，所述的蚊子的幼虫和蛹的混合物是通过以下方式获得：将含有蚊子的幼虫和蛹的培养基质或培养液倒入到筛网中，用水进行过滤，去除基质，得到蚊子的幼虫和蛹的混合物。Preferably, the mixture of mosquito larvae and pupae is obtained in the following manner: the culture substrate or culture solution containing mosquito larvae and pupae is poured into a screen, filtered with water, and the matrix is removed to obtain mosquito larvae and Mixture of pupae.
所述的筛网是20目的筛网。The sieve is 20 mesh.
优选，将含有蚊子的幼虫和蛹的培养基质或培养液倒入到筛网中，用水进行过滤，去除基质或培养液，得到蚊子的幼虫和蛹的混合物，将蚊子的幼虫和蛹的混合物倒入到冰水中，蛹漂浮于水面，幼虫沉于冰水中，然后用带筛网的过滤勺将蛹收集，再收集下层的幼虫，得到分离的蛹和幼虫。Preferably, pour the culture medium or the culture solution containing the larvae and pupae of mosquitoes into the sieve, filter with water, remove the matrix or culture fluid, obtain the mixture of larvae and pupae of mosquitoes, pour the mixture of larvae and pupae of mosquitoes Put into the ice water, the pupae float on the water surface, and the larvae sink in the ice water, then collect the pupae with a filter spoon with a sieve, and then collect the larvae in the lower layer to obtain separated pupae and larvae.
本发明人发现，当蚊子的幼虫和蛹放于冰水中，幼虫由于被冰冻一瞬间会自动下沉，而蛹则会漂于水面，当捞出蛹后，冰水的温度回升至常温(25℃)的时候，幼虫又会自动上浮上来。The inventor found that when the larvae and pupae of mosquitoes were placed in ice water, the larvae would sink automatically due to being frozen for a moment, while the pupae would float on the water surface. After the pupae were pulled out, the temperature of the iced water rose to normal temperature (25 ℃), the larvae will automatically float up again.
因此，本发明能够有效的分离蚊子的幼虫和蛹，提高蚊子幼虫和蛹的分离效率，省时省力，不损伤蛹。Therefore, the present invention can effectively separate mosquito larvae and pupae, improve the separation efficiency of mosquito larvae and pupae, save time and labor, and do not damage pupae.
附图说明：Description of drawings:
图1是蚊子的幼虫和蛹的分离效果图。Figure 1 is a diagram showing the separation effect of mosquito larvae and pupae.
具体实施方式：Detailed ways:
以下实施例是对本发明的进一步说明，而不是对本发明的限制。The following examples are to further illustrate the present invention, rather than limit the present invention.
实施例1：Example 1:
将饲养的实验用含有蚊子的幼虫和蛹的培养基质倒入到底壁带有20目筛孔的过滤杯中，用水进行过滤，去除基质(基质从筛孔中滤出)，得到蚊子的幼虫和蛹的混合物，将含蚊子的幼虫和蛹的混合物的过滤杯置于到冰水中，冰水通过筛孔进入到过滤杯中，等待10秒后，蛹漂浮于水面，幼虫沉于冰水中，然后用带筛网的过滤勺将蛹收集，收集完后，提起过滤杯，过滤杯底即为幼虫。收集的蛹为活体，可以孵化成成虫，因此可以用于蛹期实验或培养成虫实验需要。The experiment of raising will be poured into the filter cup with 20 mesh sieve holes on the bottom wall with the culture medium containing the larvae and pupae of mosquitoes, filter with water, remove matrix (matrix is filtered out from the sieve hole), obtain the larvae and pupae of mosquitoes. For the mixture of pupae, place the filter cup containing the mixture of mosquito larvae and pupae into ice water, and the ice water enters the filter cup through the sieve holes. After waiting for 10 seconds, the pupae float on the water surface, and the larvae sink in the ice water, and then Use a filter spoon with a sieve to collect the pupae. After collecting, lift the filter cup, and the bottom of the filter cup is the larvae. The collected pupae are living and can hatch into adults, so they can be used for pupal stage experiments or adult culture experiments.
对照：将蚊子的幼虫和蛹的混合物倒于常温(25℃)的水中，蚊子的幼虫和蛹都漂浮于水面上，蚊子的幼虫并不沉入水中或水底。Control: Pour the mixture of mosquito larvae and pupae into water at normal temperature (25°C), both mosquito larvae and pupae float on the water surface, and mosquito larvae do not sink into the water or the bottom of the water.
实施例2：Example 2:
将200只白纹伊蚊蛹和200只白纹伊蚊幼虫混合在水盘中的培养液饲养，然后再倒入到底壁带有20目筛孔的过滤杯中，用水进行冲滤，去除培养液，得到蚊子的幼虫和蛹的混合物，将含蚊子的幼虫和蛹的混合物的过滤杯置于到冰水中，冰水通过筛孔进入到过滤杯中，等待10秒后，蛹漂浮于水面，幼虫沉于冰水中，然后用带筛网的过滤勺将蛹收集，收集完后，提起过滤杯，过滤杯底即为幼虫。收集的蛹为活体，可以孵化成成虫，因此可以用于蛹期实验或培养成虫实验需要。200 Aedes albopictus pupae and 200 Aedes albopictus larvae were mixed in the culture solution in the water tray and then poured into a filter cup with a 20-mesh sieve on the bottom wall, rinsed with water to remove the culture medium. liquid to obtain the mixture of mosquito larvae and pupae, place the filter cup containing the mixture of mosquito larvae and pupae into ice water, and the ice water enters the filter cup through the sieve hole, after waiting for 10 seconds, the pupae float on the water surface, The larvae sink in ice water, and then the pupae are collected with a filter spoon with a sieve. After the collection, the filter cup is lifted, and the bottom of the filter cup is the larvae. The collected pupae are living and can hatch into adults, so they can be used for pupal stage experiments or adult culture experiments.
实验结果如图1所示，从图1可以看出，幼虫和蛹得到了很好的分离。The experimental results are shown in Figure 1, from which it can be seen that the larvae and pupae were well separated.
实施例3：Example 3:
将实施例2的白纹伊蚊的幼虫和蛹的混合物倒于冰水中，等待10秒后，蛹漂浮于水面上，而蚊子的幼虫沉于水中。The mixture of Aedes albopictus larvae and pupae in Example 2 was poured into ice water, and after waiting for 10 seconds, the pupae floated on the water surface, while the mosquito larvae sank in the water.
对照：将白纹伊蚊的幼虫和蛹的混合物倒于常温(25℃)的水中，蚊子的幼虫和蛹都漂浮于水面上，蚊子的幼虫并不沉入水底。Control: Pour the mixture of Aedes albopictus larvae and pupae into water at normal temperature (25°C), both the mosquito larvae and pupae float on the water surface, and the mosquito larvae do not sink to the bottom of the water.
Claims (4)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910703749.5A CN110560249A (en) | 2019-07-31 | 2019-07-31 | Method for quickly separating mosquito pupae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910703749.5A CN110560249A (en) | 2019-07-31 | 2019-07-31 | Method for quickly separating mosquito pupae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110560249A true CN110560249A (en) | 2019-12-13 |
Family
ID=68773879
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910703749.5A Pending CN110560249A (en) | 2019-07-31 | 2019-07-31 | Method for quickly separating mosquito pupae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110560249A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118058237A (en) * | 2024-03-26 | 2024-05-24 | 中山大学 | Separation method of male and female mosquito pupae |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1651414A (en) * | 2005-01-18 | 2005-08-10 | 上海应用技术学院 | Separation and purification method of pyriproxyfen |
| CN102499182A (en) * | 2011-10-19 | 2012-06-20 | 武汉市疾病预防控制中心 | Integral type mosquito raising device |
| CN202285912U (en) * | 2011-10-19 | 2012-07-04 | 武汉市疾病预防控制中心 | Integrated mosquito-raising apparatus |
| CN102910737A (en) * | 2012-10-20 | 2013-02-06 | 北京工业大学 | Device and method for culturing and separating chironomus larvas for reducing sludge |
| CN103340179A (en) * | 2013-06-25 | 2013-10-09 | 广东省农业科学院植物保护研究所 | Phyllotreta striolata insect pest situation investigation method and special separation device |
| US9180464B2 (en) * | 2012-03-20 | 2015-11-10 | Oxitec Limited | Sorting apparatus for arthropods and method of use thereof |
| JP5824450B2 (en) * | 2009-07-14 | 2015-11-25 | インベスティガシオン・イ・コメルシアリサシオン・ビオテクノロヒカ・オメビット・ソシエダッド・アノニマ | Methods for obtaining fatty acids from insect larvae |
| CN208879055U (en) * | 2018-09-30 | 2019-05-21 | 湖北远志黄粉虫养殖有限责任公司 | A kind of screening plant of worm pupa for separating yellow meal worm, larva and frass |
-
2019
- 2019-07-31 CN CN201910703749.5A patent/CN110560249A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1651414A (en) * | 2005-01-18 | 2005-08-10 | 上海应用技术学院 | Separation and purification method of pyriproxyfen |
| JP5824450B2 (en) * | 2009-07-14 | 2015-11-25 | インベスティガシオン・イ・コメルシアリサシオン・ビオテクノロヒカ・オメビット・ソシエダッド・アノニマ | Methods for obtaining fatty acids from insect larvae |
| CN102499182A (en) * | 2011-10-19 | 2012-06-20 | 武汉市疾病预防控制中心 | Integral type mosquito raising device |
| CN202285912U (en) * | 2011-10-19 | 2012-07-04 | 武汉市疾病预防控制中心 | Integrated mosquito-raising apparatus |
| US9180464B2 (en) * | 2012-03-20 | 2015-11-10 | Oxitec Limited | Sorting apparatus for arthropods and method of use thereof |
| CN102910737A (en) * | 2012-10-20 | 2013-02-06 | 北京工业大学 | Device and method for culturing and separating chironomus larvas for reducing sludge |
| CN103340179A (en) * | 2013-06-25 | 2013-10-09 | 广东省农业科学院植物保护研究所 | Phyllotreta striolata insect pest situation investigation method and special separation device |
| CN208879055U (en) * | 2018-09-30 | 2019-05-21 | 湖北远志黄粉虫养殖有限责任公司 | A kind of screening plant of worm pupa for separating yellow meal worm, larva and frass |
Non-Patent Citations (1)
| Title |
|---|
| 杨俊德等: "实验室斯氏按蚊的饲养方法", 《医学动物防制》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118058237A (en) * | 2024-03-26 | 2024-05-24 | 中山大学 | Separation method of male and female mosquito pupae |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wilson | Development of sponges from dissociated tissue cells | |
| Caswell et al. | Extraction of cysts and eggs of Heterodera schachtii from soil with an assessment of extraction efficiency | |
| US20120017834A1 (en) | Apparatus and method for controlling maturation of aquatically hatched insects | |
| CN110560249A (en) | Method for quickly separating mosquito pupae | |
| CN104957116B (en) | A kind of method for trapping fragrant-flowered garlic maggot Adult worms producting eggs | |
| CN109430178B (en) | Method for prolonging life of adult arma chinensis | |
| CN101843228A (en) | Observation device for catching mites and nematodes and use method and preparation method thereof | |
| CN207411307U (en) | A kind of mosquito lures egg apparatus | |
| CN209711170U (en) | A kind of trapping device for aphids aphids | |
| CN208016722U (en) | Yellow meal worm collects pupa sieve | |
| CN213695402U (en) | An easy-to-hang pest control lamp | |
| CN102487898B (en) | A kind of cultivation method and cultivation device of coral fertilized eggs before attachment | |
| CN105028361B (en) | A kind of method trapped and monitor fragrant-flowered garlic maggot adult | |
| CN202310940U (en) | Novel basin type trapper | |
| CN221429979U (en) | Novel pear borer trapper | |
| CN209628399U (en) | A kind of paddlefish seed rearing device | |
| CN206284187U (en) | A kind of device for trapping spotted wing drosophila adult | |
| CN206196699U (en) | A kind of shrimp-cultivation pool blowdown apparatus | |
| CN207870153U (en) | A kind of drosophila killing trapping device | |
| Anderson et al. | Development and field testing of a quantitative technique for extracting bean leaf beetle larvae and pupae from soil | |
| CN118058237B (en) | Separation method of male and female mosquito pupae | |
| CN206699185U (en) | A kind of machine that worm material separation is carried out with pipeline mode | |
| CN204518947U (en) | A kind of 6 hole peach fruit borer lure trapper | |
| CN220756209U (en) | Component and device for capturing ampullaria gigas manufactured by mineral water bottle | |
| SU1161051A1 (en) | Method of extracting larvae of synantrop flies from feed substrate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 510260 No. 105 West Xingang Road, Guangzhou, Guangdong, Haizhuqu District Applicant after: Institute of zoology, Guangdong Academy of Sciences Address before: 510260 No. 105 West Xingang Road, Guangzhou, Guangdong, Haizhuqu District Applicant before: Guangdong Institute of Applied Biological Resources |
|
| CB02 | Change of applicant information | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20191213 |
|
| WD01 | Invention patent application deemed withdrawn after publication |