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CN110382705A - 丙谷二肽的制备方法、丙谷二肽制备用酶及应用 - Google Patents

丙谷二肽的制备方法、丙谷二肽制备用酶及应用 Download PDF

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CN110382705A
CN110382705A CN201880014704.0A CN201880014704A CN110382705A CN 110382705 A CN110382705 A CN 110382705A CN 201880014704 A CN201880014704 A CN 201880014704A CN 110382705 A CN110382705 A CN 110382705A
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aminoacidesters
acyltransferase
seq
gene
preparation
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CN110382705B (zh
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傅荣昭
李振伟
张贵慰
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Bontac Bio-Engineering (shenzhen) Co ltd
Bontac Invitrolife Bio Technology Shenzhen Co ltd
Zhongshan Bangtai Hesheng Biotechnology Co ltd
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Bontac Invitrolife Bio Technology Shenzhen Co ltd
Jiangxi Bonzymes Biotechnology Co ltd
Bontac Bio-Engineering (shenzhen) Co ltd
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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Abstract

本发明提供了一种丙谷二肽的制备方法，包括在α‑氨基酸酯酰基转移酶存在下，以L‑丙氨酸甲酯盐酸盐和L‑谷氨酰胺为底物配制反应液，调节所述反应液的pH为7.0‑9.0，在恒定温度20‑40℃下反应后，收集得到丙谷二肽；所述α‑氨基酸酯酰基转移酶来源于脑膜败血伊丽莎白菌；所述α‑氨基酸酯酰基转移酶的氨基酸序列包括如SEQ ID NO：1‑SEQ ID NO：6中任意一项所示的氨基酸序列。该制备方法高效简便，成本低，转化率高，绿色环保，可以广泛适用于工业化规模生产。本发明还提供了丙谷二肽制备用酶及应用。

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PCT国内申请，说明书已公开。

Claims (15)

  1. PCT国内申请，权利要求书已公开。
CN201880014704.0A 2018-09-26 2018-09-26 丙谷二肽的制备方法、丙谷二肽制备用酶及应用 Active CN110382705B (zh)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112831451A (zh) * 2019-11-25 2021-05-25 中国科学院微生物研究所 产丙谷二肽的工程菌及其构建方法与应用
CN113817790A (zh) * 2021-10-18 2021-12-21 精晶药业股份有限公司 一种固定化酶制备丙氨酰谷氨酰胺的方法
CN116286567A (zh) * 2022-09-27 2023-06-23 合肥工业大学 一株产α-氨基酸酯酰基转移酶的重组大肠埃希氏菌及其构建方法和应用
CN116640819A (zh) * 2023-07-20 2023-08-25 凯莱英生命科学技术(天津)有限公司 α-氨基酸脂酰基转移酶的制备方法及其在二肽合成上的应用
CN118546847A (zh) * 2024-05-09 2024-08-27 合肥工业大学 一株产α-氨基酸酯酰基转移酶的重组大肠杆菌及其构建方法和应用

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115160164A (zh) * 2022-05-06 2022-10-11 大连医诺生物股份有限公司 一种复合丙氨酸甲酯盐酸盐的制备方法及应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104480075A (zh) * 2014-11-20 2015-04-01 江苏诚信药业有限公司 一种催化合成丙谷二肽的生物酶及其制备方法和应用
CN105274174A (zh) * 2015-11-30 2016-01-27 精晶药业股份有限公司 一种生物酶转化制备丙谷二肽的方法
CN106834394A (zh) * 2017-01-20 2017-06-13 天津科技大学 一种丙谷二肽的制备方法
WO2018121458A1 (zh) * 2016-12-30 2018-07-05 大连医诺生物股份有限公司 编码丙谷二肽生物合成酶的基因及其应用

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104480075A (zh) * 2014-11-20 2015-04-01 江苏诚信药业有限公司 一种催化合成丙谷二肽的生物酶及其制备方法和应用
CN105274174A (zh) * 2015-11-30 2016-01-27 精晶药业股份有限公司 一种生物酶转化制备丙谷二肽的方法
WO2018121458A1 (zh) * 2016-12-30 2018-07-05 大连医诺生物股份有限公司 编码丙谷二肽生物合成酶的基因及其应用
CN106834394A (zh) * 2017-01-20 2017-06-13 天津科技大学 一种丙谷二肽的制备方法

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112831451A (zh) * 2019-11-25 2021-05-25 中国科学院微生物研究所 产丙谷二肽的工程菌及其构建方法与应用
CN112831451B (zh) * 2019-11-25 2022-09-16 中国科学院微生物研究所 产丙谷二肽的工程菌及其构建方法与应用
CN113817790A (zh) * 2021-10-18 2021-12-21 精晶药业股份有限公司 一种固定化酶制备丙氨酰谷氨酰胺的方法
CN113817790B (zh) * 2021-10-18 2023-11-03 精晶药业股份有限公司 一种固定化酶制备丙氨酰谷氨酰胺的方法
CN116286567A (zh) * 2022-09-27 2023-06-23 合肥工业大学 一株产α-氨基酸酯酰基转移酶的重组大肠埃希氏菌及其构建方法和应用
CN116286567B (zh) * 2022-09-27 2023-09-29 合肥工业大学 一株产α-氨基酸酯酰基转移酶的重组大肠埃希氏菌及其构建方法和应用
CN116640819A (zh) * 2023-07-20 2023-08-25 凯莱英生命科学技术(天津)有限公司 α-氨基酸脂酰基转移酶的制备方法及其在二肽合成上的应用
CN116640819B (zh) * 2023-07-20 2023-10-13 凯莱英生命科学技术(天津)有限公司 α-氨基酸脂酰基转移酶的制备方法及其在二肽合成上的应用
CN118546847A (zh) * 2024-05-09 2024-08-27 合肥工业大学 一株产α-氨基酸酯酰基转移酶的重组大肠杆菌及其构建方法和应用

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WO2019157823A1 (zh) 2019-08-22

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