CN110317759A - The anaerobism electricity production bacterial strain of one plant of degradable phenol and its application - Google Patents

Abstract

The anaerobism electricity production bacterial strain of one plant of degradable phenol and its application are related to one plant of anaerobism electricity production bacterial strain and its application.It is to solve existing phenol degrading bacterial strain to be applied in MFC, the lower problem of the coulombic efficiency of MFC.The bacterial strain is enterobacteria Enterobacter sp.A4, is deposited in China typical culture collection center, and the deposit date is on April 11st, 2019, deposit number was CCTCC No:M 2019249.Using the strain construction microbiological fuel cell degradation of phenol, for improving the coulombic efficiency of microbiological fuel cell.The present invention is for the phenol pollutant in degrading waste water.

Description

An anaerobic electrogenic strain capable of degrading phenol and its application

technical field

The invention relates to an anaerobic electricity-producing strain and its application.

Background technique

Phenol is a protoplasmic highly toxic aromatic compound, which is often used as a raw material and an intermediate in the chemical industry. The chemical structure of phenols is stable and difficult to degrade, and direct discharge will cause serious harm to organisms and the environment. At present, there are physical methods (adsorption method, solvent extraction method), chemical method (oxidation method, precipitation method and photocatalysis method), biological method (activated sludge method, enzyme treatment method) and so on to treat phenol-containing wastewater. Physical and chemical methods are suitable for high-concentration phenol-containing wastewater, and have the disadvantages of high investment and large losses, while biological treatment of phenol-containing wastewater is a low-cost and environmentally friendly pollution remediation method. In recent years, in the research on microbial degradation of phenol, the phenol-degrading bacteria screened and isolated from phenol-contaminated environments include Pseudomonas sp., Pseudomonas putida, and Bacillus sphaericus. (Bacillussphaericus), Rhodococcus sp., Rhizobia, Acinetobactersp., Comamonas testoterone, Bacillus cereus and the like.

However, these strains are not suitable for use in microbial fuel cell (MFC) degradation of phenol. Coulombic efficiency reflects the proportion of MFC degraded organic matter used to generate electricity. The higher the coulombic efficiency, the higher the conversion rate of battery energy. Existing phenol-degrading strains are applied to MFC, and the coulombic efficiency of MFC is low, which in turn affects the conversion rate of battery energy.

SUMMARY OF THE INVENTION

The invention aims to solve the problem of low coulombic efficiency of MFC when the existing phenol-degrading strain is applied to MFC, and provides an anaerobic electricity-producing strain capable of degrading phenol and its application.

The anaerobic electrogenic strain capable of degrading phenol of the present invention is Enterobacter Enterobacter sp.A4, which is preserved in the China Collection Center for Type Cultures (CCTCC), and the preservation address is Wuhan University, Wuhan, and the preservation date is April 11, 2019, and the preservation The number is CCTCC No: M 2019249.

The Enterobacter sp.A4 of the present invention is a gram-negative bacterium, straight rod-shaped, with a cell size of (1.1-1.5 μm)×(2.0-6.0 μm), single or paired, and moving with or without peristaltic flagella. Motile, spore-free, non-capsulated, facultatively anaerobic, forming red, larger colonies on MacConkey plates.

The Enterobacter sp.A4 of the present invention can decompose glucose and lactose to produce acid and gas, and acetate can be used as the only carbon source, but citrate cannot be used. The H ₂ S test was negative, the indole test was negative, the methyl red test was negative, the VP test was positive, and the citrate utilization test was positive. Oxidase was negative, phenylalanine deaminase test was negative, ornithine and arginine dihydrolase were positive, and lysine decarboxylase was negative.

The Enterobacter sp.A4 of the present invention can use NH ₄ NO ₃ as the optimum nitrogen source, sucrose as the optimum carbon source, the optimum initial pH value is 8, and the optimum growth temperature is 37°C. The optimum temperature for this strain to degrade phenol was 23℃. The optimal inoculation amount is 10%.

According to the 16S rDNA sequence alignment analysis, the Enterobacter A4 of the present invention has extremely high homology with various species in the genus Enterobacter sp., and the homology is more than 99.5%. Enterobacter A4 belongs to the genus Enterobacter sp. by combining the morphological characteristics, growth conditions, and physiological and biochemical identification results.

In the invention, the phenol-containing anaerobic stage industrial wastewater sample is used as a research sample, and the anaerobic electricity-producing microorganisms that can efficiently degrade phenol are separated and purified from the anode of the MFC in the stable period; The growth conditions and phenol degradation conditions of the strain were optimized to obtain the most suitable growth and phenol degradation conditions for the strain. Finally, it is applied in MFC to obtain more superior performance, and it also provides ideas for the efficient and low-consumption treatment of refractory organics such as phenols.

Enterobacter A4 of the present invention can tolerate a certain concentration of Pb ²⁺ (0.2 mg/L), and when a high concentration of Pb ²⁺ (0.2 mg/L) exists, Enterobacter A4 still maintains a high phenol degradation ability. The strain produced electricity in the stable phase of growth, and the phenol degradation rate reached 67.37% at 64h, and the coulombic efficiency (CE) was 17.8%. The MFC constructed by this strain can efficiently degrade phenol, and also has obvious advantages in energy conversion efficiency. Therefore, it is of great significance in the application of MFC to treat phenol-containing wastewater.

The Enterobacter sp.A4 of the present invention belongs to the genus Enterobacter sp., and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation address is Wuhan University, Wuhan, and the preservation date is April 11, 2019, and the preservation The number is CCTCC No: M 2019249.

Description of drawings

Fig. 1 is the curve that the MFC voltage of strain changes with time;

Fig. 2 is the phylogenetic tree of Enterobacter A4 of the present invention;

Fig. 3 is the influence of nitrogen source on the growth of strain A4;

Figure 4 shows the effect of nitrogen sources on the phenol degradation rate of strain A4;

Figure 5 is the effect of carbon source on the growth of strain A4;

Figure 6 shows the effect of carbon source on the phenol degradation rate of strain A4;

Figure 7 is the effect of pH on the growth of strain A4;

Figure 8 is the effect of pH on the phenol degradation rate of strain A4;

Fig. 9 is the influence of inoculation amount on the growth amount of strain A4;

Figure 10 is the effect of inoculum on the phenol degradation rate of strain A4;

Figure 11 is the effect of temperature on the growth of strain A4;

Figure 12 is the effect of temperature on the phenol degradation rate of strain A4;

Figure 13 is the effect of initial phenol concentration on the growth of strain A4;

Figure 14 shows the effect of initial phenol concentration on the phenol degradation rate of strain A4;

Figure 15 shows the effect of metal ions on the growth of strain A4;

Figure 16 shows the effect of metal ions on the phenol degradation rate of strain A4;

Figure 17 is the electricity production curve of the MFC constructed by strain A4;

Figure 18 is the phenol degradation curve of MFC constructed by strain A4;

Fig. 19 is the cyclic voltammetry curve of bacterial strain A4 cultivation;

Figure 20 is a scanning electron microscope photo magnified 1000 times of the surface of the anode carbon felt;

Figure 21 is a scanning electron microscope photo magnified 3000 times of the surface of the anode carbon felt;

Figure 22 is a scanning electron microscope photo magnified by 5000 times of the surface of the anode carbon felt;

FIG. 23 is a scanning electron microscope photograph of Enterobacter A4 cells on the surface of the anode carbon felt at a magnification of 3200 times.

Detailed ways

The technical solutions of the present invention are not limited to the specific embodiments listed below, but also include any combination of specific embodiments.

Specific embodiment 1: The anaerobic electrogenic strain that can degrade phenol in this embodiment is Enterobacter Enterobactersp.A4, which is preserved in the China Collection Center for Type Cultures (CCTCC), and the preservation address is Wuhan University, Wuhan, and the preservation date is April 2019. On March 11, the deposit number was CCTCC No: M 2019249.

Enterobacter sp.A4 of the present embodiment is a Gram-negative bacterium, straight rod-shaped, with a cell size of (1.1-1.5 μm)×(2.0-6.0 μm), single or paired, and moves with peristaltic flagella or Non-motile, non-spore-free, non-capsulated, facultatively anaerobic, forming red, larger colonies on MacConkey plates.

Enterobacter sp.A4 of the present embodiment can decompose glucose and lactose to produce acid and gas, and acetate can be used as the sole carbon source, but citrate cannot be used. The H ₂ S test was negative, the indole test was negative, the methyl red test was negative, the VP test was positive, and the citrate utilization test was positive. Oxidase was negative, phenylalanine deaminase test was negative, ornithine and arginine dihydrolase were positive, and lysine decarboxylase was negative.

The Enterobacter sp.A4 of the present embodiment can use NH ₄ NO ₃ as the optimum nitrogen source, sucrose as the optimum carbon source, the optimum initial pH value is 8, and the optimum growth temperature is 37°C. The optimum temperature for this strain to degrade phenol was 23℃. The optimal inoculation amount is 10%.

Embodiment 2: The screening method of Enterobacter sp.A4 of the present embodiment is:

The microbial fuel cell was chosen as a two-chamber MFC, and the volume of both chambers was 600 mL. The anode electrode adopts carbon felt modified by ferric chloride (5cm×5cm, effective area is 12.56cm ² ), and the cathode electrode adopts carbon brush. Before using the carbon brush, use 1mol/L HCl and 1mol/L NaCl soaked for 24h. The cathode chamber and the anode chamber are connected by a salt bridge, and the interface is insulated. The external varistor box (0～9999Ω) in the circuit adopts the resistance of 1000Ω. The voltage generated during the experiment was directly recorded by a dual-channel voltage acquisition system (PISO813), and the data was measured every 60s.

N ₂ was fed for 20 min before cell startup to ensure anaerobic conditions. First, the sewage in the aeration tank and the sewage in the anaerobic tank are mixed in a volume ratio of 1:1 to form anaerobic sewage. /L) and phenol (500 mg/L). The entire battery anode assembly was placed on a 30°C constant temperature magnetic stirrer for stirring to maintain the homogeneity of the anode solution. The sewage of the anaerobic tank was collected from the A ² /O anaerobic tank of the Wenchang Sewage Treatment Plant in Harbin, Heilongjiang Province, and the sewage of the aeration tank was also collected from the Wenchang Sewage Treatment Plant in Harbin, Heilongjiang Province.

Under sterile conditions, the sterilized anaerobic solid medium is injected into the anaerobic tube to keep it in a sealed state, and then the anaerobic tube is placed horizontally and rolled slowly in a low-temperature cold water bath to make the medium evenly solidified inside. The tube wall is thus made into an anaerobic rolling tube. The MFC carbon felt in a stable state was taken out, put into a sterilized anaerobic tube, and enriched with an anaerobic liquid medium at 37°C for 4 days. After enrichment, the bacterial liquid was injected into the anaerobic rolling tube by the dilution method, and the rolling tube was rolled to make the bacterial liquid evenly distributed, sealed and placed in an anaerobic incubator filled with nitrogen at 37 °C. Colonies were purified by step-by-step streak isolation. Finally, the obtained pure bacteria were inoculated into anaerobic solid medium, cultured at 37°C for 4 days, and stored in a refrigerator at 4°C.

The bacterial liquid of the isolated strain was scanned by cyclic voltammetry, and the electrochemical activity of the isolated strain was judged by the redox peak. The real-time voltage recording software of microbial fuel cell is PIS0813. The electrochemical workstation software is CHI760d. The cyclic voltammogram of the voltage change and the electrochemical activity of the strain was detected by origin8.0.

The curve of MFC voltage versus time is shown in Figure 1. It can be seen from Figure 1 that the voltage from the beginning of the recording of the voltage data to 59h is stable at about 0.696V, and after a rapid drop in voltage, it quickly rises to about 0.717V. This process is generally considered to be the accumulation of electricity-producing microorganisms on the electrode surface. The process of forming biofilms. As shown in Figure 1, the voltage gradually stabilized after 98h. The maximum output voltage of the MFC system is 0.797V. The calculated maximum current density is 634.55 mA/m ² and the maximum power density is 510 mW/m ² .

A total of 5 pure anaerobic electricity-producing strains were isolated, and the electricity-producing properties of each strain are shown in Table 1. Of the 5 strains, only A4 had the ability to generate electricity.

Table 1 Electricity-generating characteristics of anaerobic electricity-producing strains

Molecular identification of the isolated strain A4 shows that the 16S rDNA sequence of the strain A4 is 1411 bp in length, and its sequence is shown in SEQ ID NO: 1 in the sequence listing. The sequencing results were compared with the 16S rDNA sequence in GenBank for homology, and the constructed phylogenetic tree was shown in Figure 2 to determine the species relationship of the strains. The results of homology analysis showed that the sequence had extremely high homology with various species in Enterobacter sp., and the homology was more than 99.5%. The strain A4 was determined to belong to Enterobacter sp. by combining the morphological characteristics, growth conditions, and physiological and biochemical identification results of the bacteria.

Embodiment 3: Optimization of the growth of phenol-degradable anaerobic electrogenic bacteria and the optimization of phenol degradation conditions

On an anaerobic operating table, the strains were inoculated at 30°C and cultured on a shaker with a separation medium of 160 r/min for 48 h, and 3 mL of the culture solution was taken and centrifuged (8000 r/min) for 3 times in a centrifuge tube. Put into 50mL of physiological saline to prepare bacterial suspension. The bacterial suspension was inoculated into the inorganic salt medium for 48 hours, and the effects of different biological characteristics (nitrogen source, carbon source, pH value, inoculum amount, initial phenol concentration, temperature and metal ions) on the growth of the strain and the degradation of phenol were studied. . When the bacteria were inserted into the shake flask, 2 repetitions were performed, and 3 repetitions were performed when the growth amount and the phenol degradation rate were determined, and then the average value was taken.

The determination method of phenol degradation rate is as follows:

Take 3mL of sewage every 4h and centrifuge (12000r/min), take out the supernatant of the culture medium after centrifugation, and measure the phenol concentration. Distilled water was used as the blank sample. The amount of water, sample and each reagent added was: 0.2mL ammonia buffer (pH 9.8), 0.4mL 4% 4-aminoantipyrine solution, 0.4mL 8% potassium ferricyanide solution , 0.1mL sample, 3.9mL distilled water. After the mixture was left standing for 10 min, the absorbance was measured at a wavelength of 510 nm, and then the phenol concentration corresponding to the measured absorbance was found using the phenol concentration standard curve, and the phenol degradation rate was calculated according to formula (1).

In this embodiment, NH ₄ Cl, (NH ₄ ) ₂ SO ₄ , NH ₄ NO ₃ and NaNO ₃ are respectively used as nitrogen sources. After strain A4 was cultured for 48h, when the nitrogen source was NH ₄ Cl, the measured OD ₆₀₀ was 1.35. When the nitrogen source was NH ₄ NO ₃ , (NH ₄ ) ₂ SO ₄ and NaNO ₃ , the OD ₆₀₀ was 0.89, 0.67, and 0.36, respectively. The effect of nitrogen source on the growth of strain A4 is shown in Figure 3, and the effect of nitrogen source on the degradation rate of phenol is shown in Figure _{4. NH4NO3} as the nitrogen source has the highest degradation rate of phenol, and the degradation rate of phenol is the highest. The influence of , in turn, is (NH ₄ ) ₂ SO ₄ , NH ₄ Cl and NaNO ₃ . According to comprehensive analysis, NH ₄ NO ₃ can be used as the best nitrogen source for strain A4.

In this embodiment, sucrose, lactose, glucose and sodium acetate are used as the external carbon sources for experiments. After 48 hours of culture, the effect of carbon source on the growth of strain A4 is shown in Figure 5, and the effect of carbon source on the phenol degradation rate is shown in Figure 5. 6, lactose had the most obvious effect on the growth promotion of the strain, and the OD ₆₀₀ reached 0.73. Next is sucrose with an _OD600 of 0.68. When the additional carbon source was sucrose, the phenol degradation rate of the strain was the highest, reaching 29.5%. The possible reason is that sucrose and strain A4 have co-metabolism in phenol degradation. Considering the growth of the strain, the degradation rate of phenol and the economic benefit, sucrose was selected as the best carbon source for the degradation of phenol by the strain.

The initial pH value of the medium has an important influence on the growth of microorganisms and the degradation of organic matter. As shown in Figure 7, with the increase of the initial pH value, the growth amount of strain A4 showed a trend of increasing first and then decreasing. Studies have shown that the strain A4 is adapted to the weak alkaline environment, and the most suitable initial pH value for the growth of the strain is 8. As shown in Fig. 8, when the pH value is 8.0, the phenol degradation rate of the strain is the highest, and the phenol degradation rate of the strain reaches 50% under this pH condition for 48 hours. To sum up, the pH value of 8 was selected as the optimal initial pH of strain A4.

Figure 9 and Figure 10 show the effect of inoculation amount on the growth of the strain and the phenol degradation rate. With the increase of inoculation amount, the lag period gradually shortened, the nutrient consumption rate increased, and the phenol degradation rate also increased. When the inoculation amount is 2% to 5%, due to the small amount of bacteria and sufficient nutrition, the bacterial growth rate is faster. When the inoculation amount was 10%, the strain growth OD ₆₀₀ and phenol degradation efficiency were 0.35 and 23.58%, respectively, which were higher than those of 1% (0.17 and 12.53%), 5% (0.28 and 21.36%) and 20% ( 0.28 and 14.61%). While the inoculum amount exceeded 10%, the OD ₆₀₀ dropped sharply, and the ability of bacteria to degrade phenol was also significantly weakened. The reason is that when the inoculation amount is high, carbon source competition will be formed, which will inhibit the reproduction of the strain and affect the degradation rate. Taking all into consideration, the optimal inoculation amount of strain A4 is 10%.

In the process of biological wastewater treatment, the temperature is closely related to the enzymatic activity of the bacterial cells. Within a suitable temperature range, the physiological activities of microorganisms are vigorous, which helps to improve the oxidative degradation and anabolic capacity of organic pollutants. It can be seen from Figure 11 and Figure 12 that when the culture temperature reaches 37 °C, the OD ₆₀₀ value is 1.059, and the growth rate reaches the maximum; but at this temperature, the degradation rate of phenol by strain A4 is 16.82%, which is significantly lower than 23. ℃ to the degradation rate of phenol, and the phenol degradation rate of the strain decreased with the increase of temperature. So 37℃ was the optimum temperature for the growth of strain A4, and 23℃ was the optimum temperature for the strain to degrade phenol.

Sometimes the content of phenol in the wastewater discharged from the factory is too high, and whether it can tolerate high concentrations of phenol is an important performance indicator to measure the actual application conditions of the strain. Therefore, in this embodiment, the tolerance of strain A4 to high concentrations of phenol was studied. The initial phenol concentrations set in this experiment were 500 mg/L, 1000 mg/L and 2000 mg/L, respectively. The study showed that, as shown in Figures 13 and 14, the growth of A4 was the best in 500 mg/L phenol wastewater, and the phenol concentration at this time was most favorable for the degradation of p-phenol. With the increase of the initial phenol concentration, the growth of the strain was obviously inhibited. When the phenol concentration reached 2000 mg/L, the phenol degradation ability of the strain almost disappeared, indicating that the strain A4 was not suitable for application at higher concentrations of phenol.

Figure 15 and Figure 16 respectively show four kinds of heavy metal ions Cd ²⁺ , Co ²⁺ , Pb ²⁺ and Cu ²⁺ , the mass concentrations of each metal ion are 0.01mg/L, 0.02mg/L, 0.05mg/L, The effects of 0.1mg/L, 0.2mg/L and 0.4mg/L on the growth amount and phenol degradation rate of strain A4. In FIGS. 15 and 16 , ■ represents Cd ²⁺ , ● represents Co ²⁺ , ▲ represents Pb ²⁺ , and ▼ represents Cu ²⁺ . It can be seen from Figure 15 that among the four heavy metal ions, the strain has the strongest tolerance to Pb ²⁺ , and the OD ₆₀₀ measured at 48h reaches 0.4, followed by Co ²⁺ and Cu ²⁺ , and the strain to Cd ²⁺ . The least tolerance. With the continuous increase of the concentration of four heavy metal ions, the growth of the strain showed different situations. When the concentration exceeded 0.05mg/L, the tolerance of the strain to Co ²⁺ , Cd ²⁺ and Cu ²⁺ gradually weakened with the increase of the concentration. The tolerance of strain A4 to Pb ²⁺ showed a trend of increasing first and then decreasing, and the OD ₆₀₀ reached the maximum when the concentration was 0.2 mg/L.

Figure 16 shows that when the concentration of Cd ²⁺ , Co ²⁺ and Pb ²⁺ increased from 0.01mg/L to 0.4mg/L, the inhibitory effect on the degradation rate of phenol was continuously enhanced. Among them, Cd ²⁺ had the most obvious effect. 48h phenol degradation rate decreased by 15.70%. However, when the concentration of Pb ²⁺ is lower than 0.2mg/L, it can significantly promote the degradation of phenol, and the degradation rate of phenol also shows an increasing trend with the increase of concentration. The degradation efficiency of phenol reaches the highest level, and then the degradation rate of phenol decreases sharply with the increase of Pb ²⁺ concentration. It can be seen that strain A4 can tolerate a certain concentration of Pb ²⁺ , and in the presence of a high concentration of Pb ²⁺ (0.2 mg/L), strain A4 still maintains a high phenol degradation ability.

Specific embodiment four: This embodiment uses Enterobacter Enterobacter sp.A4 to construct MFC

Glucose (1000mg/L) and phenol (500mg/L) were used as substrates, strain A4 was inoculated into the anode, MFC was started, and the electricity-producing ability of the strain was verified. The result of the operation is shown in Figure 17. The operation of strain A4 in MFC can be roughly divided into two periods, namely the period of rapid voltage rise and the period of relatively stable voltage. The starting voltage was 0.66V, and after a rapid increase of the voltage for about 125h, the voltage leveled off, reaching a maximum voltage of 718mV at 124h. The rapid increase in voltage after medium change is due to the important role of the biofilm already formed on the anode surface in MFC. The culture medium was replaced three times, and the voltage was relatively stable at 157h. With the operation of the battery, the gap between the value of the voltage in the stable period and the peak line gradually narrowed, which may be due to the secretion of redox substances by the bacteria, which effectively increased the electron transfer rate.

As shown in Figure 18, when strain A4 was inoculated into MFC containing phenol (500 mg/L), the phenol degradation rate gradually increased with the increase of time. The degradation rate of phenol reached the maximum value of 67.37% at 64h, and then showed a downward trend. The calculated coulombic efficiency was 17.8%. The Coulombic efficiency of strain A4 was higher than other phenol-fueled microbial fuel cells. Coulombic efficiency reflects the proportion of MFC degraded organic matter used to generate electricity. The greater the Coulombic efficiency, the higher the conversion rate of battery energy. Therefore, in terms of energy conversion efficiency, the MFC constructed by strain A4 has certain advantages.

Exploring the electrogenicity of strains at different growth stages is of great significance to the optimization of strain culture conditions. Cyclic voltammetry was used to determine whether the strain had electrochemical activity. Figure 19 is a cyclic voltammetry diagram of strain A4 cultured, in which the solid line represents the bacterial sample and the dotted line represents the control. The curve containing strain A4 has a pair of oxidation peaks and reduction peaks in the stable phase. The oxidation peak is located at -0.7mV to -0.3mV, and the reduction peak is around 0.5mV to 0.7mV, which indicates that the strain has electrogenic activity and the strain is stable. Period electricity.

Embodiment 5: In this embodiment, scanning electron microscope observation and analysis of the anode carbon felt is carried out

The MFC anode carbon felt in the stable period was taken out, and the carbon felt was prepared for scanning electron microscope by tert-butanol and glutaraldehyde method. Specific operation: After fixing the carbon felt with 2.5% glutaraldehyde, rinse it three times with a phosphate buffer solution with a concentration of 0.1 mol/L pH 6.8, and then with a concentration of 50%, 70%, 80%, 90% It was dehydrated with 100% ethanol, then replaced with tert-butanol, and finally dried on a freeze dryer. After the dried samples were sprayed with gold, the surface morphology was observed with a scanning electron microscope at an accelerating voltage of 5.00 KV.

20 to 23 show the morphology of the biofilm and A4 cells observed by SEM. The anode carbon felt in the MFC has an interdigitated network structure, which is beneficial to the attachment and growth of strain A4, thus facilitating the formation of biofilms. Figure 22 is the SEM image of the carbon felt magnified by 5000 times after the MFC runs for 300 hours. A large number of rod-shaped cells are attached to the anode surface, and the growth is relatively dense, which provides intuitive evidence for the electricity production capacity of strain A4. No similar nanometers were observed outside the cell membrane. A derived structure of the wire, the strains are simply in contact with the electrodes. Strain A4 may facilitate electron transfer in microorganisms by secreting certain redox mediators.

sequence list

<110> Harbin University of Science and Technology

<120> An anaerobic electrogenic strain that can degrade phenol and its application

<160> 1

<210> 1

<211> 1411

<212> DNA

<213> Enterobacter

<220>

<223> Enterobacter A4 16SrDNA

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taatgtctgg gaaactgcct gatggagggg gataactact ggaaacggta gctaataccg 120

cataatgtcg caagaccaaa gagggggacc ttcgggcctc ttgccatcag atgtgcccag 180

atgggattag ctagtaggtg gggtaacggc tcacctaggc gacgatccct agctggtctg 240

agaggatgac cagccacact ggaactgaga cacggtccag actcctacgg gaggcagcag 300

tggggaatat tgcacaatgg gcgcaagcct gatgcagcca tgccgcgtgt atgaagaagg 360

ccttcgggtt gtaaagtact ttcagcgggg aggaaggtgt tgtggttaat aaccacagca 420

attgacgtta cccgcagaag aagcaccggc taactccgtg ccagcagccg cggtaatacg 480

gagggtgcaa gcgttaatcg gaattactgg gcgtaaagcg cacgcaggcg gtctgtcaag 540

tcggatgtga aatccccggg ctcaacctgg gaactgcatt cgaaactggc aggctggagt 600

cttgtagagg ggggtagaat tccaggtgta gcggtgaaat gcgtagagat ctggaggaat 660

accggtggcg aaggcggccc cctggacaaa gactgacgct caggtgcgaa agcgtgggga 720

gcaaacagga ttagataccc tggtagtcca cgccgtaaac gatgtcgatt tggaggttgt 780

gcccttgagg cgtggcttcc ggagctaacg cgttaaatcg accgcctggg gagtacggcc 840

gcaaggttaa aactcaaatg aattgacggg ggcccgcaca agcggtggag catgtggttt 900

aattcgatgc aacgcgaaga accttacctg gtcttgacat ccacagaact ttccagagat 960

ggattggtgc cttcgggaac tgtgagacag gtgctgcatg gctgtcgtca gctcgtgttg 1020

tgaaatgttg ggttaagtcc cgcaacgagc gcaaccctta tcctttgttg ccagcggtta 1080

ggccgggaac tcaaaggaga ctgccagtga taaactggag gaaggtgggg atgacgtcaa 1140

gtcatcatgg cccttacgac cagggctaca cacgtgctac aatggcgcat acaaagagaa 1200

gcgacctcgc gagagcaagc ggacctcata aagtgcgtcg tagtccggat tggagtctgc 1260

aactcgactc catgaagtcg gaatcgctag taatcgtaga tcagaatgct acggtgaata 1320

cgttcccggg ccttgtacac accgcccgtc acaccatggg agtgggttgc aaaagaagta 1380

ggtagcttaa ccttcgggag ggcgctacca c 1411

Claims (5)

1. an anaerobic electrogenic bacterial strain that can degrade phenol, is characterized in that this bacterial strain is Enterobacter Enterobactersp.A4, is preserved in China Type Culture Collection Center, and the preservation address is Wuhan University, Wuhan City, and the preservation date is April, 2019 On the 11th, the deposit number was CCTCC No: M 2019249. 2. the application of the anaerobic electrogenic strain that can degrade phenol according to claim 1 in degrading phenol in waste water. 3. application according to claim 2 is characterized in that utilizing described bacterial strain to construct microbial fuel cell to degrade phenol. 4. The application according to claim 2, wherein the strain is used to improve the coulombic efficiency of a microbial fuel cell. 5. application according to claim 2 is characterized in that described bacterial strain still maintains phenol degradation ability when Pb ²⁺ concentration is 0.2mg/L.