CN110150368B - A kind of preservation method for prolonging the shelf life of banana - Google Patents
A kind of preservation method for prolonging the shelf life of banana Download PDFInfo
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- 235000018290 Musa x paradisiaca Nutrition 0.000 title claims abstract description 81
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000004321 preservation Methods 0.000 title claims abstract description 12
- 240000005561 Musa balbisiana Species 0.000 title 1
- 241000234295 Musa Species 0.000 claims abstract description 150
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 74
- 235000021015 bananas Nutrition 0.000 claims abstract description 70
- 239000000284 extract Substances 0.000 claims abstract description 32
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 21
- 235000009566 rice Nutrition 0.000 claims abstract description 21
- 239000000243 solution Substances 0.000 claims abstract description 16
- 239000007921 spray Substances 0.000 claims abstract description 14
- 230000006378 damage Effects 0.000 claims abstract description 12
- 239000007864 aqueous solution Substances 0.000 claims abstract description 9
- 238000001035 drying Methods 0.000 claims abstract description 7
- 238000005507 spraying Methods 0.000 claims abstract description 3
- 240000007594 Oryza sativa Species 0.000 claims abstract 5
- 239000010903 husk Substances 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 239000000706 filtrate Substances 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 10
- 239000008367 deionised water Substances 0.000 claims description 9
- 229910021641 deionized water Inorganic materials 0.000 claims description 9
- 239000004744 fabric Substances 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 9
- 238000000605 extraction Methods 0.000 claims description 7
- 238000002386 leaching Methods 0.000 claims description 7
- 238000004140 cleaning Methods 0.000 claims description 4
- 230000007547 defect Effects 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 238000002390 rotary evaporation Methods 0.000 claims description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 206010059866 Drug resistance Diseases 0.000 abstract description 2
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 239000003755 preservative agent Substances 0.000 abstract description 2
- 231100000331 toxic Toxicity 0.000 abstract description 2
- 230000002588 toxic effect Effects 0.000 abstract description 2
- 230000000844 anti-bacterial effect Effects 0.000 abstract 1
- 239000003899 bactericide agent Substances 0.000 abstract 1
- 239000003640 drug residue Substances 0.000 abstract 1
- 230000002335 preservative effect Effects 0.000 abstract 1
- 241000209094 Oryza Species 0.000 description 16
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 13
- 239000005977 Ethylene Substances 0.000 description 13
- 238000003860 storage Methods 0.000 description 13
- 230000000694 effects Effects 0.000 description 11
- 230000005855 radiation Effects 0.000 description 9
- 230000005070 ripening Effects 0.000 description 8
- OSVXSBDYLRYLIG-UHFFFAOYSA-N dioxidochlorine(.) Chemical compound O=Cl=O OSVXSBDYLRYLIG-UHFFFAOYSA-N 0.000 description 6
- 102000016938 Catalase Human genes 0.000 description 5
- 108010053835 Catalase Proteins 0.000 description 5
- 102000030523 Catechol oxidase Human genes 0.000 description 4
- 108010031396 Catechol oxidase Proteins 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 4
- 239000005969 1-Methyl-cyclopropene Substances 0.000 description 3
- SHDPRTQPPWIEJG-UHFFFAOYSA-N 1-methylcyclopropene Chemical compound CC1=CC1 SHDPRTQPPWIEJG-UHFFFAOYSA-N 0.000 description 3
- 239000004155 Chlorine dioxide Substances 0.000 description 3
- 238000010306 acid treatment Methods 0.000 description 3
- 235000019398 chlorine dioxide Nutrition 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 230000029058 respiratory gaseous exchange Effects 0.000 description 3
- 102000004316 Oxidoreductases Human genes 0.000 description 2
- 108090000854 Oxidoreductases Proteins 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 235000012055 fruits and vegetables Nutrition 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000005557 antagonist Substances 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 230000009920 chelation Effects 0.000 description 1
- -1 citric acid compound Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 229910001431 copper ion Inorganic materials 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 108091054761 ethylene receptor family Proteins 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 150000007523 nucleic acids Chemical group 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
- A23B7/00—Preservation of fruit or vegetables; Chemical ripening of fruit or vegetables
- A23B7/015—Preserving by irradiation or electric treatment without heating effect
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
- A23B7/00—Preservation of fruit or vegetables; Chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by group A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by group A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Preparation Of Fruits And Vegetables (AREA)
- Storage Of Fruits Or Vegetables (AREA)
Abstract
Description
技术领域technical field
本发明涉及果品保存技术领域,特别涉及一种延长香蕉货架期的保鲜方法。The invention relates to the technical field of fruit preservation, in particular to a fresh-keeping method for prolonging the shelf life of bananas.
背景技术Background technique
香蕉采收以后需要经过催熟才能食用,催熟之前,香蕉果皮为绿色,果肉涩而硬,不能食用,但抗病性强,不易腐烂。经过催熟,香蕉果皮变黄、果肉变软,才具有食用价值,但成熟的香蕉极易腐烂,一般货架期只有2-3天,如果不能在2-3天内售出或食用,香蕉会很快丧失商品价值和食用价值,给销售商和消费者均造成经济损失。After harvesting, bananas need to be ripened before they can be eaten. Before ripening, the banana peel is green, and the pulp is astringent and hard, which is inedible, but has strong disease resistance and is not easy to rot. After ripening, banana peels turn yellow and pulp becomes soft, so they have edible value, but ripe bananas are very perishable, and the general shelf life is only 2-3 days. If they cannot be sold or eaten within 2-3 days, bananas will be very The commodity value and edible value are quickly lost, causing economic losses to both sellers and consumers.
导致香蕉成熟的物质是一种叫做乙烯的内源激素,香蕉采收以后会通过自身产生的乙烯自动催熟,因此用一定措施减少贮藏环境中的乙烯含量,可以推迟香蕉成熟,延长保鲜期,但这并不能减少香蕉成熟后腐烂,香蕉成熟后腐烂主要由真菌病原菌引起,现行的水果保鲜方法主要使用化学杀菌剂处理控制病害,减少腐烂发生,但化学杀菌剂作为采后香蕉的保鲜剂可能带来毒性残留、抗药性以及环保问题,所以寻找高效、快速、无污染的香蕉保鲜剂已受到普遍重视。The substance that causes bananas to ripen is an endogenous hormone called ethylene. After the bananas are harvested, they will automatically ripen through the ethylene produced by themselves. Therefore, taking certain measures to reduce the ethylene content in the storage environment can delay the ripening of bananas and prolong the preservation period. However, this does not reduce the rot of bananas after ripening. The rot of bananas after ripening is mainly caused by fungal pathogens. The current method of fruit preservation mainly uses chemical fungicides to control the disease and reduce the occurrence of rot. Bringing toxic residues, drug resistance and environmental protection problems, so the search for efficient, fast and pollution-free banana preservatives has received widespread attention.
张珊珊等采用二氧化氯处理香蕉果实,结果表明二氧化氯可以抑制香蕉内源乙烯的产生,推迟呼吸跃变高峰出现时间,使呼吸强度明显降低,但是二氧化氯处理提高了多酚氧化酶的活性;1-甲基环丙烯(1-MCP)是一种无毒的乙烯拮抗剂,能与果蔬组织中的乙烯受体蛋白发生不可逆的结合,起到抑制乙烯的作用,但1-MCP必须在果实尚未发生呼吸跃变和产生可自动催化的乙烯之前进行施用才能抑制呼吸和乙烯的释放;华南农业大学公开了一种香蕉保鲜专利,采用48-60℃热水浸泡处理香蕉可达到控制成熟香蕉腐烂,延长货架期的保鲜效果,但热水处理仅适合处于常温下存放时间不超过48小时的青香蕉。Zhang Shanshan et al. treated banana fruit with chlorine dioxide. The results show that chlorine dioxide can inhibit the production of endogenous ethylene in bananas, delay the appearance of the climacteric peak, and significantly reduce the respiration intensity. However, chlorine dioxide treatment increases the production of polyphenol oxidase. Activity; 1-Methylcyclopropene (1-MCP) is a non-toxic ethylene antagonist that can irreversibly bind to ethylene receptor proteins in fruit and vegetable tissues to inhibit ethylene, but 1-MCP must Respiration and ethylene release can be inhibited by application before the fruit has undergone climacteric change and autocatalytic ethylene is produced; South China Agricultural University has disclosed a patent for banana preservation, which can control ripening by soaking bananas in hot water at 48-60°C. Bananas rot and prolong shelf life, but hot water treatment is only suitable for green bananas that are stored at room temperature for no more than 48 hours.
虽然国内外有关学者对香蕉的采后生理和保鲜技术进行了大量的研究,但是到目前为止,仍然没有得到令人满意的结果,香蕉的后熟软化和储运保鲜仍是亟待研究的主要问题。Although domestic and foreign scholars have carried out a lot of research on the postharvest physiology and fresh-keeping technology of bananas, so far, no satisfactory results have been obtained. .
发明内容SUMMARY OF THE INVENTION
本发明的目的在于提供一种安全环保的延长香蕉货架期的保鲜方法,减缓香蕉成熟腐烂,延长香蕉货架期,提高香蕉的商品价值和食用价值。The object of the present invention is to provide a safe and environmentally friendly method for extending the shelf life of bananas, slow down the ripening and decay of bananas, prolong the shelf life of bananas, and improve the commodity value and edible value of bananas.
本发明提供的一种延长香蕉货架期的保鲜方法,包括以下步骤:A fresh-keeping method for extending the shelf life of bananas provided by the invention comprises the following steps:
步骤1:以香蕉色卡为标准,挑选初始状态为4的香蕉,并要求大小一致,没有机械损伤与黑星点,用干净的布擦干净,放置于有孔的容器中;Step 1: Use the banana color card as the standard, select the bananas with the initial state of 4, and require the same size, no mechanical damage and black stars, wipe them with a clean cloth, and place them in a perforated container;
步骤2:将香蕉置于UV-C灯管下方,进行UV-C辐照,辐照过程中对香蕉表面喷洒柠檬酸溶液,关闭UV-C光源,晾干;Step 2: Put the banana under the UV-C lamp tube, and irradiate it with UV-C. During the irradiation process, spray citric acid solution on the surface of the banana, turn off the UV-C light source, and dry it;
步骤3:将UV-C处理后的香蕉完全浸没稻壳中保存。Step 3: Fully immerse the UV-C treated banana in the rice husk for preservation.
优选的是,步骤2中UV-C灯管功率为36W,辐照距离为50cm,辐照时间为1min;Preferably, in step 2, the power of the UV-C lamp tube is 36W, the irradiation distance is 50cm, and the irradiation time is 1min;
优选的是,步骤2中柠檬酸溶液的浓度为0.2%;Preferably, the concentration of the citric acid solution in step 2 is 0.2%;
优选的是,步骤3所述稻壳为用含香蕉叶提取物的水溶液通过喷雾装置进行喷雾处理6-12h后晾干所得;Preferably, the rice husks described in step 3 are obtained by spraying an aqueous solution containing banana leaf extract through a spray device for 6-12 hours and then drying;
优选的是,所述香蕉叶提取物的制备方法包括以下步骤:Preferably, the preparation method of the banana leaf extract comprises the following steps:
(1)预处理:选取生长状况良好无不良缺陷的香蕉叶,清洗后切小片,风干,然后用粉碎机打成粒度小于50目的粉末;(1) Pretreatment: select banana leaves with good growth conditions and no defects, cut into small pieces after cleaning, air-dry, and then use a pulverizer to make a powder with a particle size of less than 50 meshes;
(2)预提:取上述粉末置于去离子水中浸泡24-30h;(2) Pre-extraction: take the above powder and place it in deionized water to soak for 24-30h;
(3)浸提:上述预提液在水浴锅中浸提60-90min;(3) leaching: the above-mentioned pre-extraction solution is leached in a water bath for 60-90min;
(4)过滤:将浸提液用四层纱布过滤;(4) Filtration: filter the extract with four layers of gauze;
(5)浓缩滤液:所得滤液放入旋转蒸发仪中在温度为70~75℃的情况下旋转蒸去水(5) Concentrated filtrate: the obtained filtrate is put into a rotary evaporator, and the water is distilled off by rotary evaporation at a temperature of 70 to 75° C.
分得浸膏状提取物;The extract is obtained in the form of extract;
(6)烘干:在30~40℃烘箱中烘干,放入干燥瓶中保存。(6) Drying: dry in an oven at 30-40 °C, and store in a drying bottle.
优选的是,上述步骤(2)中香蕉叶粉末和去离子水按照料液比1:10-12添加,采用Preferably, in above-mentioned step (2), banana leaf powder and deionized water are added according to the ratio of material to liquid 1:10-12, using
超声条件浸泡;Soak in ultrasonic conditions;
优选的是,上述步骤(3)浸提温度为45~50℃;Preferably, the leaching temperature in the above step (3) is 45-50°C;
优选的是,上述步骤(4)过滤后的残渣进行重复浸提过滤,重复过滤后的滤液合并Preferably, the residue after the filtration of the above step (4) is subjected to repeated leaching and filtration, and the filtrate after repeated filtration is combined
进行步骤(5)浓缩滤液;Carry out step (5) and concentrate the filtrate;
本发明通过上述操作在不破坏香蕉叶中具有延长香蕉保鲜期的有效成分活性的同时The present invention has the active ingredient activity of prolonging the fresh-keeping period of bananas in the banana leaves without destroying the activity of the active ingredients through the above-mentioned operations.
对其进行充分的提取。Extract it sufficiently.
优选的是,所述香蕉叶提取物的水溶液中香蕉叶提取物的质量分数为10-35%;Preferably, the mass fraction of the banana leaf extract in the aqueous solution of the banana leaf extract is 10-35%;
优选的是,将被稻壳浸没的香蕉容器置于0-4℃环境中保存。Preferably, the banana container submerged in rice husks is stored in an environment of 0-4°C.
本发明至少包括以下有益效果:The present invention includes at least the following beneficial effects:
初始状态为4的香蕉略带黄色状态,表示淀粉正逐渐转变为糖分,在该时段进行香蕉处理,可以最大限度的减缓香蕉褐变以及腐烂速率,提高香蕉的保鲜期;The banana with an initial state of 4 is slightly yellow, indicating that the starch is gradually being converted into sugar. Banana treatment during this period can slow down the browning and decay rate of the banana to the greatest extent, and improve the fresh-keeping period of the banana;
UV-C辐照可以破坏微生物细胞膜与核酸结构使遗传物质失去活性,致使微生物细胞死去或失去繁殖能力,控制病原微生物的繁殖,防止腐烂从而延缓果蔬采后衰老;UV-C irradiation can destroy the microbial cell membrane and nucleic acid structure, make the genetic material inactive, cause the microbial cells to die or lose their ability to reproduce, control the reproduction of pathogenic microorganisms, prevent rot and delay the post-harvest aging of fruits and vegetables;
柠檬酸是生物个体天然存在的有机酸,是一类酚类铜螯合剂,而香蕉发生酶促褐变的主要原因是一种含有铜离子的氧化酶的多酚氧化酶,因此柠檬酸通过螯合作用抑制多酚氧化酶的活力从而减缓香蕉的褐变腐烂速度;Citric acid is a naturally occurring organic acid in living organisms and is a type of phenolic copper chelator. The main reason for enzymatic browning of bananas is a polyphenol oxidase containing copper ion oxidase. The synergistic effect inhibits the activity of polyphenol oxidase so as to slow down the browning and rotten speed of bananas;
UV-C辐照和柠檬酸同时处理香蕉,加速了柠檬酸同多酚氧化酶螯合作用,同时柠檬酸提供的酸性环境也加速了UV-C辐照对微生物细胞膜的破坏作用,二者协同作用,极大的减缓了香蕉的褐变腐烂速度;Simultaneous treatment of bananas with UV-C irradiation and citric acid accelerated the chelation of citric acid with polyphenol oxidase, and the acidic environment provided by citric acid also accelerated the destruction of microbial cell membranes by UV-C irradiation. It can greatly slow down the browning and rotten speed of bananas;
稻壳中具有良好的韧性、多孔性,将UV-C辐照和柠檬酸处理后的香蕉置于稻壳中保存一方面可以减缓香蕉存放过程中的产生的机械损伤,另一方面稻壳还可以起到透气、吸湿、抗氧化、防止霉变等作用,更重要的稻壳中的多孔结构可以吸附香蕉所产生的乙烯,从而减少环境中外源乙烯的含量,进一步减缓存储过程中产生的外源乙烯对香蕉的进一步催熟。Rice husks have good toughness and porosity. Putting the bananas after UV-C irradiation and citric acid treatment in rice husks can reduce the mechanical damage during banana storage on the one hand, and on the other hand, rice husks also It can play the role of breathability, moisture absorption, anti-oxidation, and anti-mildew. More importantly, the porous structure in the rice husk can absorb the ethylene produced by bananas, thereby reducing the content of exogenous ethylene in the environment and further slowing down the exogenous ethylene produced during storage. Source ethylene for further ripening of bananas.
通过将稻壳用含香蕉叶提取物的水溶液通过喷雾装置进行喷雾处理6-12h后,稻壳中的孔道结构中吸附了香蕉叶提取物,香蕉叶提取物对维持香蕉内过氧化氢酶的内环境有着非常重要的作用,通过将UV-C辐射结合柠檬酸复合处理过的香蕉浸没与含有香蕉叶提取物的稻壳中保存,有助于恢复过氧化氢酶活性,极大的提高了香蕉的保鲜效果,减缓香蕉褐变腐烂速度,极大的延长了香蕉的保鲜期。After the rice husk is sprayed with an aqueous solution containing banana leaf extract through a spray device for 6-12 hours, the banana leaf extract is adsorbed in the pore structure in the rice husk, and the banana leaf extract is effective in maintaining the capacity of catalase in banana. The internal environment plays a very important role. By immersing the bananas treated with UV-C radiation combined with citric acid and storing them in rice husks containing banana leaf extracts, it helps to restore the activity of catalase and greatly improves the efficiency of the treatment. The fresh-keeping effect of bananas can slow down the browning and rotten speed of bananas, and greatly prolong the fresh-keeping period of bananas.
本发明的其它优点、目标和特征将部分通过下面的说明体现,部分还将通过对本发明的研究和实践而为本领域的技术人员所理解。Other advantages, objects, and features of the present invention will appear in part from the description that follows, and in part will be appreciated by those skilled in the art from the study and practice of the invention.
具体实施方式Detailed ways
下面对本发明做进一步的详细说明,以令本领域技术人员参照说明书文字能够据以实施。The present invention will be further described in detail below, so that those skilled in the art can implement it with reference to the description.
为更清楚的说明本发明的技术方案,将以具体的实施例进一步说明。In order to illustrate the technical solutions of the present invention more clearly, specific embodiments will be further described.
实施例1Example 1
以香蕉色卡为标准,挑选初始状态为4的香蕉,大小一致,没有机械损伤与黑星点的香蕉,用干净的布擦干净,放置于有孔的容器中;将香蕉置于36W的UV-C灯管下方50cm,进行UV-C辐照,辐照时间为1min,常温保存。观察记录香蕉储存情况,见表1;Using the banana color card as the standard, select bananas with an initial state of 4, the same size, without mechanical damage and black star points, wipe them with a clean cloth, and place them in a perforated container; put the bananas in a 36W UV light 50cm below the -C lamp tube, UV-C irradiation is carried out, the irradiation time is 1min, and it is stored at room temperature. Observe and record the banana storage situation, see Table 1;
实施例2Example 2
以香蕉色卡为标准,挑选初始状态为4的香蕉,大小一致,没有机械损伤与黑星点的香蕉,用干净的布擦干净,放置于有孔的容器中;喷洒0.2%柠檬酸溶液,晾干,常温保存。观察记录香蕉储存情况,见表1;Using the banana color card as the standard, select bananas with an initial state of 4, the same size, without mechanical damage and black stars, wipe them with a clean cloth, and place them in a perforated container; spray 0.2% citric acid solution, Dry and store at room temperature. Observe and record the banana storage situation, see Table 1;
实施例3Example 3
以香蕉色卡为标准,挑选初始状态为4的香蕉,大小一致,没有机械损伤与黑星点的香蕉,用干净的布擦干净,放置于有孔的容器中;将香蕉置于36W的UV-C灯管下方50cm,进行UV-C辐照,辐照时间为1min;喷洒0.2%柠檬酸溶液,晾干,常温保存。观察记录香蕉储存情况,见表1;Using the banana color card as the standard, select bananas with an initial state of 4, the same size, without mechanical damage and black star points, wipe them with a clean cloth, and place them in a perforated container; put the bananas in a 36W UV light 50cm below the -C lamp tube, carry out UV-C irradiation, the irradiation time is 1min; spray 0.2% citric acid solution, air dry, and store at room temperature. Observe and record the banana storage situation, see Table 1;
实施例4Example 4
以香蕉色卡为标准,挑选初始状态为4的香蕉,大小一致,没有机械损伤与黑星点的香蕉,用干净的布擦干净,放置于有孔的容器中;将香蕉置于36W的UV-C灯管下方50cm,进行UV-C辐照,辐照时间为1min,辐照过程中对香蕉表面喷洒浓度为0.2%的柠檬酸溶液,关闭UV-C光源,晾干,常温保存,观察记录香蕉储存情况,见表1;Using the banana color card as the standard, select bananas with an initial state of 4, the same size, without mechanical damage and black star points, wipe them with a clean cloth, and place them in a perforated container; put the bananas in a 36W UV light 50cm below the -C lamp tube, irradiate with UV-C, the irradiation time is 1min, spray citric acid solution with a concentration of 0.2% on the surface of the banana during the irradiation process, turn off the UV-C light source, dry it, store it at room temperature, and observe Record the banana storage situation, see Table 1;
实施例5Example 5
以香蕉色卡为标准,挑选初始状态为4的香蕉,大小一致,没有机械损伤与黑星点的香蕉,用干净的布擦干净,放置于有孔的容器中;将香蕉置于36W的UV-C灯管下方50cm,进行UV-C辐照,辐照时间为1min,辐照过程中对香蕉表面喷洒浓度为0.2%的柠檬酸溶液,关闭UV-C光源,晾干;将UV-C处理后的香蕉完全浸没稻壳中常温保存。观察记录香蕉储存情况,见表1;Using the banana color card as the standard, select bananas with an initial state of 4, the same size, without mechanical damage and black star points, wipe them with a clean cloth, and place them in a perforated container; put the bananas in a 36W UV light 50cm below the -C lamp tube, irradiate with UV-C, the irradiation time is 1min, spray citric acid solution with a concentration of 0.2% on the surface of the banana during the irradiation process, turn off the UV-C light source, and dry; The processed bananas are completely immersed in rice husks and stored at room temperature. Observe and record the banana storage situation, see Table 1;
实施例6Example 6
以香蕉色卡为标准,挑选初始状态为4的香蕉,大小一致,没有机械损伤与黑星点的香蕉,用干净的布擦干净,放置于有孔的容器中;将香蕉置于36W的UV-C灯管下方50cm,进行UV-C辐照,辐照时间为1min,辐照过程中对香蕉表面喷洒浓度为0.2%的柠檬酸溶液,关闭UV-C光源,晾干;将UV-C处理后的香蕉完全浸没稻壳中0-4℃保存。观察记录香蕉储存情况,见表1;Using the banana color card as the standard, select bananas with an initial state of 4, the same size, without mechanical damage and black star points, wipe them with a clean cloth, and place them in a perforated container; put the bananas in a 36W UV light 50cm below the -C lamp tube, irradiate with UV-C, the irradiation time is 1min, spray citric acid solution with a concentration of 0.2% on the surface of the banana during the irradiation process, turn off the UV-C light source, and dry; The treated bananas were completely immersed in rice husks and stored at 0-4°C. Observe and record the banana storage situation, see Table 1;
实施例7Example 7
步骤1.制备香蕉叶提取液:选取生长状况良好无不良缺陷的香蕉叶,清洗后切小片,风干,然后用粉碎机打成粒度小于50目的粉末;取香蕉叶粉末和去离子水按照料液比1:10置于去离子水中浸泡24-30h;预提液在水浴锅中45~50℃浸提60-90min;将浸提液用四层纱布过滤;对过滤残渣在水浴锅中45~50℃重复浸提60-90min,合并两次所得滤液放入旋转蒸发仪中在温度为70~75℃的情况下旋转蒸去水分得浸膏状提取物,在30~40℃烘箱中烘干,放入干燥瓶中保存备用。Step 1. Preparation of banana leaf extract: select banana leaves with good growth conditions and no defects, cut into small pieces after cleaning, air-dry, and then use a pulverizer to make powder with a particle size of less than 50 meshes; take banana leaf powder and deionized water according to the feed liquid Immerse in deionized water at a ratio of 1:10 for 24-30h; extract the pre-extraction solution in a water bath at 45-50°C for 60-90min; filter the extraction solution with four layers of gauze; Repeat the leaching at 50°C for 60-90min, combine the two obtained filtrates, put them into a rotary evaporator, and rotate to evaporate the water at a temperature of 70-75°C to obtain an extract-like extract, which is dried in an oven at 30-40°C. , stored in a dry bottle for later use.
步骤2.将制备的香蕉叶提取物加去离子水制成15%的香蕉叶提取物水溶液,通过喷雾装置用香蕉叶提取物水溶液对稻壳进行喷雾处理6h,取出晾干备用;Step 2. Add deionized water to the prepared banana leaf extract to make a 15% banana leaf extract aqueous solution, spray the rice husks with the banana leaf extract aqueous solution for 6h by a spray device, and take it out to dry for use;
步骤3.以香蕉色卡为标准,挑选初始状态为4的香蕉,大小一致,没有机械损伤与黑星点的香蕉,用干净的布擦干净,放置于有孔的容器中;将香蕉置于36W的UV-C灯管下方50cm,进行UV-C辐照,辐照时间为1min,辐照过程中对香蕉表面喷洒浓度为0.2%的柠檬酸溶液,关闭UV-C光源,晾干;将UV-C处理后的香蕉完全浸没用香蕉叶提取物水溶液处理过的稻壳中常温保存。观察记录香蕉储存情况,见表1;Step 3. Using the banana color card as the standard, select bananas with an initial state of 4, the same size, without mechanical damage and black star points, wipe them with a clean cloth, and place them in a perforated container; put the bananas in a 50cm below the 36W UV-C lamp tube, carry out UV-C irradiation, the irradiation time is 1min, spray the citric acid solution with a concentration of 0.2% on the surface of the banana during the irradiation process, turn off the UV-C light source, and dry it; The bananas treated with UV-C were completely immersed in rice husks treated with an aqueous solution of banana leaf extract and stored at room temperature. Observe and record the banana storage situation, see Table 1;
实施例8Example 8
处理方法同实施例7,区别在于0-4℃放置,观察记录香蕉储存情况,见表1;The treatment method is the same as in Example 7, except that the difference is that 0-4 ° C is placed, and the storage situation of the bananas is observed and recorded, as shown in Table 1;
实施例9Example 9
步骤1.制备香蕉叶提取液:选取生长状况良好无不良缺陷的香蕉叶,清洗后切小片,风干,然后用粉碎机打成粒度小于50目的粉末;取香蕉叶粉末和去离子水按照料液比1:10置于去离子水中浸泡24-30h;预提液用加热回流的方法浸提60-90min;将浸提液用四层纱布过滤;对过滤残渣在水浴锅中45-50℃重复浸提60-90min,合并两次所得滤液放入旋转蒸发仪中在温度为70-75℃的情况下旋转蒸去水分得浸膏状提取物,在30-40℃烘箱中烘干,放入干燥瓶中保存备用。Step 1. Preparation of banana leaf extract: select banana leaves with good growth conditions and no defects, cut into small pieces after cleaning, air-dry, and then use a pulverizer to make powder with a particle size of less than 50 meshes; take banana leaf powder and deionized water according to the feed liquid The ratio of 1:10 is placed in deionized water for 24-30h; the pre-extract is extracted by heating and refluxing for 60-90min; the extract is filtered with four layers of gauze; the filtration residue is repeated in a water bath at 45-50°C Leaching for 60-90min, the filtrate obtained by combining twice is put into a rotary evaporator, and the water is evaporated by rotary evaporation at a temperature of 70-75 ℃ to obtain an extract-like extract, dried in a 30-40 ℃ oven, put into Store in a dry bottle for later use.
其余步骤同实施例7,观察记录香蕉储存情况,见表1;All the other steps are with embodiment 7, observe and record banana storage situation, see Table 1;
表1实施例1-9香蕉储存情况记录表Table 1 embodiment 1-9 banana storage situation record table
由表1可以得出,对香蕉进行UV-C辐射、柠檬酸处理均可以在一定程度上延长香蕉的保鲜期,但是UV-C辐射、柠檬酸同时处理的效果好于分开处理,将UV-C辐射、柠檬酸同时处理的香蕉置于稻壳中可以显著延长香蕉保鲜期,但是由于UV-C辐射结合柠檬酸复合使用在抑制香蕉呼吸强度和内源乙烯的产生的同时也导致了香蕉抗氧化酶系统遭到破坏,香蕉超氧化物歧化酶和过氧化氢酶活性降低,因此UV-C辐射结合柠檬酸复合处理有很好的保鲜效果,当其一旦开始褐变腐烂,则速度则会较不进行UV-C辐射更加迅速,香蕉叶提取物对维持香蕉内过氧化氢酶的内环境有着非常重要的作用,通过将UV-C辐射结合柠檬酸复合处理过的香蕉浸没与含有香蕉叶提取物的稻壳中保存,有助于恢复过氧化氢酶活性,极大的提高了香蕉的保鲜效果,减缓香蕉褐变腐烂速度,极大的延长了香蕉的保鲜期。Can draw from Table 1, carry out UV-C radiation, citric acid treatment to banana and all can prolong the fresh-keeping period of banana to a certain extent, but the effect of UV-C radiation, citric acid treatment simultaneously is better than separate treatment, will UV-C radiation, citric acid treat simultaneously. The bananas treated with C radiation and citric acid at the same time can significantly prolong the fresh-keeping period of bananas when placed in rice husks. However, the combined use of UV-C radiation combined with citric acid inhibits the respiration intensity of bananas and the production of endogenous ethylene, and also leads to banana resistance. The oxidase system is destroyed, and the activities of superoxide dismutase and catalase in bananas are reduced. Therefore, UV-C radiation combined with citric acid compound treatment has a good preservation effect. Once it begins to brown and rot, the speed will increase. Compared with no UV-C radiation, banana leaf extract has a very important role in maintaining the internal environment of catalase in bananas. By combining UV-C radiation combined with citric acid, the immersion of bananas treated with citric acid is combined with banana leaves. Preserving the extract in the rice husk helps to restore catalase activity, greatly improves the fresh-keeping effect of bananas, slows down the browning and rotten speed of bananas, and greatly prolongs the fresh-keeping period of bananas.
尽管本发明的实施方案已公开如上,但其并不仅仅限于说明书和实施方式中所列运用,它完全可以被适用于各种适合本发明的领域,对于熟悉本领域的人员而言,可容易地实现另外的修改,因此在不背离权利要求及等同范围所限定的一般概念下,本发明并不限于特定的细节和这里的示出。Although the embodiment of the present invention has been disclosed as above, it is not limited to the application listed in the description and the embodiment, and it can be applied to various fields suitable for the present invention. For those skilled in the art, it can be easily Therefore, the invention is not limited to the specific details and illustrations herein without departing from the general concept defined by the appended claims and the scope of equivalents.
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