CN110074143A - A kind of composition and preparation method thereof for luring tea seed weevil adult - Google Patents

Abstract

The present invention discloses a kind of composition for luring tea seed weevil adult, includes: honeysuckle essential oil, Herba Andrographitis essential oil, cis- blatter alcohol, cis- 3- hexene guanidine-acetic acid rouge, 3- heptanone, hexanal and aldehyde C-9 according to volume parts；The preparation method of the composition, comprising the following steps: A, prepare organic matter mixed liquor；B, gelatin and Arabic gum preparation package solution are dissolved；C, under stirring condition, package solution is added into organic matter mixed liquor and is uniformly mixed；D, slow releasing composition is made in cohesion, solidification；Under agitation, it is 3.8 to 4 that acetic acid is added into mixed liquor made from step C and adjusts pH value in mixed liquor, generates cohesion in mixed liquor, the composition with slow release effect is made；The invention targetedly attracts tea seed weevil adult, cooperates corresponding insect trap, can effectively prevention and control oil tea garden tea seed weevil harm.Meanwhile organic solvent and chemical pesticide is not used in the invention, the ecology and environment negative effect to oil tea garden are smaller.

Description

A composition for attracting tea seed weevil adults and its preparation method

technical field

The invention relates to the field of tea seed weevil prevention and control, in particular to a composition for attracting tea seed weevil adults and a preparation method thereof.

Background technique

Teaseed weevil [TeaseedweeviI] is a tea tree pest, scientific name: Curculiochinensis Chevrolat, also known as camellia weevil or camellia weevil, through adults to supplement nutrition, lay eggs and larvae to eat seeds in the fruit to cause harm, causing camellia and tea seeds Trees drop fruit. At the same time, after the larvae are infested, the tea fruit will be filled with powdery or stick-shaped feces, and the seed kernels are prone to mold and deterioration, which has a significant impact on the quality of camellia oil. At present, almost all fruiting tea trees or tea seed trees are harmed by the tea seed weevil, and the fruit drop rate caused by it reaches more than 20%. Occurrence law: Tea seed weevil has one generation every two years in most areas of Zhejiang, and overwinters in the soil as adults or larvae. Adults are unearthed in early and mid-May, and the peak period is from early June to mid-July. Adults like dampness, weak flying ability, and feign death. Adults mate after feeding for 10 to 18 days, and lay eggs after 8 to 13 days. Eggs are laid in the seed kernel, and each female can lay 100 eggs in her lifetime. The eggs hatch in 15 days, and the larvae are 4 instars, and mature after 70 to 90 days. From August to October, the mature larva bites the fruit shell to open a 3-5mm round hole to produce fruit and overwinter in the soil. From August to September of the following year, it pupates. The pupal period is about 30 days, and the eclosion continues to survive the winter as an adult.

The control of tea seed weevil includes physical control, chemical control, biological control, etc. Each control technology has its own advantages and disadvantages. Among them, physical control can be adopted in the blooming stage of the tea seed weevil adult, using its suspended animation to use the oscillation method to carry out artificial hunting. The advantages of chemical pesticides for the prevention and control of tea seed weevil adults are simple and efficient, but the disadvantages are that there are pesticide residues in camellia oleifera fruit, food safety hazards, environmental pollution and other problems; Weevils are controlled, but there are still some limitations in the actual production application. At present, using plant volatiles or aggregation pheromones to attract and control tea garden pests is an efficient and environmentally friendly control method. In the Chinese patent application No. 201310299817.9 "A Tea Tea Using Multiple Attracting Plants to Synergistically Control Insect Pests", the following technical scheme is disclosed: artificially planting the trapping plants, Paspalum bispinatus, in the tea garden and its surroundings , ferns, wild swallows, and jaggery, play a synergistic trapping role, lure a variety of pests to the trapping plants, and then concentrate trapping and killing pests to reduce the direct damage of pests to tea trees; improve the biodiversity of tea gardens, as natural enemies The nursery is cultivated to promote the natural enemy's natural control of insect pests. With the application of the invented technology, the tea garden does not need to spray chemical pesticides, and can produce tea leaves without any pesticide residues. But the technical scheme in this patent is less targeted to the tea seed weevil, and the control effect to the tea seed weevil does not meet the actual needs of production.

SUMMARY OF THE INVENTION

The object of the present invention is to provide a composition for attracting tea seed weevil adults, and with corresponding insect traps, the invention can lure and prevent tea seed weevil adults in a targeted manner, and reduce the harm of tea seed weevil in oil tea gardens .

In order to solve this technical problem, the technical solution of the present invention is: a composition for attracting tea seed weevil adults, comprising the following materials in parts by volume:

Preferably, the following substances are included in parts by volume:

More preferably, the following materials are included in parts by volume:

The present invention improves the lure effect on the tea seed weevil by further optimizing the dosage and proportion of each material.

Further preferably, gelatin and gum arabic are also included. In the present invention, gelatin and gum arabic are added to the original composition, and the cross-linking reaction between the gelatin, gum arabic and hexanal and nonanal is used to disperse and encapsulate the mixed liquid of organic matter that has the effect of attracting tea seed weevil adults. coating, thereby realizing that the composition of the present invention has a sustained-release effect.

By adopting the above technical scheme, the beneficial effects of the present invention are: the present invention adds cis-3-hexen-1-alcohol, cis-3-hexenyl acetate, 3-heptanone, Hexanal and nonanal, together with insect traps, can effectively lure tea seed weevil adults, reduce its harm to camellia oleifera and tea seed trees, and are more pertinent and targeted; at the same time, the present invention reduces the use of organic solvents and chemical pesticides , friendly to the environment and safe to camellia oleifera and camellia seed fruit.

The object of the present invention is to provide a preparation method of a composition for attracting tea seed weevil adults, the method is easy to operate, the prepared composition has a sustained release effect, and can volatilize for a long time in the tea garden; use, reducing the negative impact on the environment.

Another purpose of the present invention is: with the corresponding insect trap, the present invention can effectively prevent and control the damage of the tea seed weevil. Compared with other control methods, the present invention saves time and labor, saves the labor cost input of Camellia oleifera garden; reduces the use of chemical pesticides, effectively reduces the pesticide residues on Camellia oleifera or tea seeds, improves the quality and safety of camellia oil, Guarantee the sustainable development of Camellia oleifera Garden.

In order to solve this technical problem, the technical solution of the present invention is: a kind of preparation method of the composition that attracts tea seed weevil adult, comprises the following steps:

A, preparation of organic mixture;

Mix honeysuckle essential oil, Andrographis paniculata essential oil, cis-3-hexen-1-ol, cis-3-hexenyl acetate, 3-heptanone, hexanal and nonanal according to the volume ratio;

B, dissolving gelatin and gum arabic to prepare encapsulation solution;

dissolving gelatin and gum arabic in water, adjusting the pH value of the gelatin and gum arabic aqueous solution to 8 to 9 to obtain a coating solution;

C. Under stirring conditions, add the coating solution to the organic mixture and mix evenly;

D, agglomeration, solidification make sustained-release composition;

Under the condition of stirring, add acetic acid to the mixed solution prepared in step C, adjust the pH value of the mixed solution to 3.8 to 4, coagulate in the mixed solution, and obtain a composition with sustained release effect.

Preferably, the mass fraction of gelatin in the coating solution in the step B is 10%, and the mass fraction of gum arabic in the coating solution in the step B is 5%. By controlling the amount of gelatin and gum arabic, the composition used to lure the tea seed weevil also has certain hexanal and nonanal in the process of volatilization, which ensures that the composition prepared by the present invention has a strong effect on the tea seed weevil adult. Effective lure.

By adopting the above-mentioned technical scheme, the beneficial effects of the present invention are: the present invention adds the coating solution that dissolves gelatin and gum arabic to the organic mixture that has the effect of attracting tea seed weevil under stirring conditions; Dispersed in the organic mixture under the action, because the coating solution is an aqueous solution, the small droplets of the coating solution are dispersed in the organic mixture, each small droplet is at the interface where the organic mixture contacts, the gelatin in the coating solution and the organic mixture solution Part of the hexanal and nonanal in the gelatin undergo cross-linking reaction to form a uniformly mixed suspension; through the coagulation and solidification steps, the gelatin further narrows the space gap and improves the thermal stability of the entire system. Coating and dispersing, so that the prepared composition not only has the effect of attracting tea seed weevil, but also has the function of sustained release, not only effectively prolonging the effective time of the prepared composition of the present invention, but also ensuring that the composition is effective against Tea seed weevil has a strong attractive effect;

Compared with the preparation of general pheromone lures in the prior art, the present invention avoids the use of organic solvents to dissolve the composition with lure effect, reduces the amount of organic solvents, and reduces environmental pollution;

The preparation steps of the present invention are simple and convenient to operate;

The gelatin and gum arabic used in the present invention can be decomposed naturally and are environmentally friendly.

Detailed ways

In order to further explain the technical solution of the present invention, the present invention will be described in detail below through specific examples.

Example 1

This embodiment discloses a composition for attracting tea seed weevil adults and a preparation method thereof, and the specific steps are as follows:

A, preparation of organic mixture;

Mix honeysuckle essential oil, Andrographis paniculata essential oil, cis-3-hexen-1-ol, cis-3-hexenyl acetate, 3-heptanone, hexanal and nonanal according to the volume ratio recorded in Table 1;

B, dissolving gelatin and gum arabic to prepare encapsulation solution;

Weigh the gum arabic and gelatin shown in Table 1, add deionized water to make the glue solution with the required concentration, and adjust the pH to 8 with 20% sodium peroxide solution.

Dissolve gelatin and gum arabic in water respectively, adjust the pH value of the aqueous solution of gelatin and gum arabic to obtain a wrapping solution; wherein the consumption of gelatin, gum arabic and water in the wrapping solution is shown in Table 1; the pH value of the wrapping solution is shown in Table 1. 2 shown;

C. Stir at 45°C for 15 minutes, add the coating solution to the organic mixture and suspend evenly;

D, agglomeration, solidification make sustained-release composition;

Under stirring conditions, add acetic acid to the mixed solution prepared in step C to adjust the pH value in the mixed solution. See Table 2 for details. Agglomeration occurs in the mixed solution. After coagulation, continue to stir for a certain period of time to obtain a composition with a slow-release effect. .

Example 2

The main differences between this example and Example 1 are shown in Table 1 and Table 2. Composition 2 with sustained release effect was prepared.

Example 3

The main differences between this example and Example 1 are shown in Table 1 and Table 2. Composition 3 with sustained release effect was prepared.

Example 4

The main differences between this example and Example 1 are shown in Table 1 and Table 2. Composition 4 with sustained release effect was prepared.

Example 5

The main differences between this example and Example 1 are shown in Table 1 and Table 2. Composition V with sustained release effect was prepared.

Example 6

The main differences between this example and Example 1 are shown in Table 1 and Table 2. Composition 6 with sustained release effect was prepared.

Compositions 1 to 6 prepared in Examples 1 to 6 above were randomly placed in the test Camellia oleifera garden through insect traps, and each example was repeated 3 times. The area of the Camellia oleifera garden is 3.33hm ² , and the main variety of Camellia oleifera planted is "Xianglin No. 1". The age, growth, and damage of tea seed weevil in this tea garden are uniform. A total of 18 test plots were set up, that is, the insect-catching data of each embodiment was the average value of the insect-catching data of 3 test plots, and the area of each plot was 667m ² . The above-mentioned compositions 1 to 6 were respectively placed in cylindrical weighing bottles without caps, each containing about 2ml of the composition; on May 16, 2018, the containing The weighing bottles of Composition 1 to Composition 6 are placed in each weevil trap. The specific structure of the weevil trap is the same as that of the patent No. ZL2018207911678 "Mobile Detachable Red Palm Weevil Trapper" or many similar structures. The layer structure is used for the trap for catching weevils, and the weighing bottle containing the composition is placed on the bottom layer of the trap. Then, the insect trap equipped with the composition was placed in the center of the experimental plot to hang, and the effect of attracting the tea seed weevil was investigated. See Table 4 for details on the lure of the compositions prepared in Examples 1 to 6; on October 17, 2018, the damage of Camellia oleifera in Examples 1 to 6 and the comparative example was investigated , the survey results are shown in Table 5.

Comparative ratio

The comparison test plot was designed, and in order to avoid interference, the distance between the comparison test plot and the embodiment test plot was 500m. See Table 5 for the investigation results of Camellia oleifera fruit damage in the comparative example.

The methods for evaluating the damage of camellia oleifera fruit in tea gardens are as follows:

Pick 100 fruits at random respectively on each test plot of Examples 1 to 6 and the camellia oleifera tree in the comparative example, bring back to the laboratory, check whether there are wormholes on the surface of the fruit, cut open the fruit and observe under a microscope whether there are larvae or eggs, and those with larvae or eggs in the fruit and wormholes on the fruit surface are recorded as insect fruit (marked as n), and the rate of insect fruit (marked as m) is calculated, see formula (1)

m=n/100*100%...(1)

Each material consumption (ml) of organic matter mixed solution in the embodiment 1 to embodiment 6 of table 1

project Honeysuckle Essential Oil Andrographis essential oil cis-3-hexen-1-ol cis-3-hexenyl acetate 3-heptanone Hexanal nonanal Example 1 20 20 13 33 6 6 6 Example 2 10 10 20 20 2 2 2 Example 3 15 18 11 31 5 8 5 Example 4 25 twenty two 16 35 8 10 10 Example 5 30 30 twenty two 40 5 2 8 Example 6 13 27 5 26 10 5 2

The amount of each substance in the coating liquid used in table 2 embodiment 1 to 6

The pH value of the adjusted solution in steps B and D in the embodiment 1 to embodiment 6 of table 3

project Step B Step D Example 1 8 3.8 Example 2 9 3.9 Example 3 8 3.8 Example 4 9 4.0 Example 5 8 3.9 Example 6 9 4.0

On June 14, 2018 (after hanging the trap for 30 days), the average quantity of the tea seed weevil caught in the trap of embodiments 1 to 6 is shown in Table 4, wherein only one-time in each trap Put in the composition for trapping the tea seed weevil.

Trapping tea seed weevil situation list in table 4 embodiment 1 to 6

project Average number of tea seed weevils (heads) Example 1 43.7 Example 2 14.5 Example 3 38.8 Example 4 36.4 Example 5 23.8 Example 6 28.8

Table 5 embodiment 1 to 6 and comparative example Camellia oleifera damage investigation form

It can be seen from Table 4 that the composition prepared by the present invention for attracting tea seed weevil adults has a certain attracting effect on tea seed weevil adults. In conjunction with Table 1 and Table 4, it can be seen that along with the ratio change between the total amount of honeysuckle essential oil and Andrographis paniculata essential oil and cis-3-hexen-1-alcohol and cis-3-hexenyl acetate total amount will affect the composition on Attractive effect of tea seed weevil, when the ratio of the total amount of honeysuckle essential oil and Andrographis paniculata essential oil to the total amount of cis-3-hexen-1-ol and cis-3-hexenyl acetate increased from 0.5 to 0.968, each The average number of tea seed weevil adults caught in the traps increased from 14.5 to 43.7; The total lipid ratio increased from 0.968 to 1.29, and the average number of tea seed weevil adults trapped in each trap decreased from 43.7 to 36.4.

As can be seen from Table 5, with corresponding insect traps, the composition prepared by the present invention can reduce the damage rate of Camellia oleifera fruit in tea gardens to a certain extent. In conjunction with Table 4 and Table 5, it can be seen that the comparative example Camellia oleifera fruit insect fruit rate is the highest, reaching 44.6%; Embodiments 1 to 6 show a similar trend with the amount of the tea seed weevil caught on average in each trap and the degree of damage to Camellia oleifera fruit. Negative correlation, when the average number of insects caught by the embodiment trap increased to 43.7 from 14.5, the insect fruit rate would reduce to 23.5% from 32.2%; when the average number of insects caught by the embodiment trap dropped to 36.4 from 43.7, The fruit rate increased from 23.5% to 29.3%.

Seen from examples 1 to 6 and comparative examples of camellia oleifera fruit damage, and with corresponding insect traps, the composition for attracting tea seed weevil adults made by the present invention can effectively reduce the damage of tea seed weevils. The insects in the examples and comparative examples Fruit rate difference is significant; And when the ratio of honeysuckle essential oil and Andrographis paniculata essential oil total amount and cis-3-hexen-1-alcohol and cis-3-hexenyl acetate total amount is 0.968, the obtained composition has the effect on tea seed weevil Adults are the best attractants.

Claims (6)

1. A composition for attracting tea seed weevil adults, characterized in that: it comprises the following materials in parts by volume: 2. A composition for attracting tea seed weevil adults as claimed in claim 1, characterized in that: it comprises the following materials in parts by volume: 3. A composition for attracting tea seed weevil adults as claimed in claim 2, characterized in that: it comprises the following materials in parts by volume: 4. A composition for attracting tea seed weevil adults as claimed in claim 1, further comprising gelatin and gum arabic. 5. A method for preparing a composition for attracting tea seed weevil adults as claimed in claim 4, characterized in that: comprising the following steps: A, preparation of organic mixture; Mix honeysuckle essential oil, Andrographis paniculata essential oil, cis-3-hexen-1-ol, cis-3-hexenyl acetate, 3-heptanone, hexanal and nonanal according to the volume ratio; B, dissolving gelatin and gum arabic to prepare encapsulation solution; dissolving gelatin and gum arabic in water, adjusting the pH value of the gelatin and gum arabic aqueous solution to 8 to 9 to obtain a coating solution; C. Under stirring conditions, add the coating solution to the organic mixture and mix evenly; D, agglomeration, solidification make sustained-release composition; Under the condition of stirring, add acetic acid to the mixed solution prepared in step C to adjust the pH value of the mixed solution from 3.8 to 4, coagulation occurs in the mixed solution, and a composition with sustained release effect is obtained. 6. The preparation method of a composition for attracting tea seed weevil adults as claimed in claim 5, characterized in that: the mass fraction of gelatin in the coating solution in the step B is 10%, and the coating in the step B The mass fraction of gum arabic in the solution is 5%.