CN109966029A - Cartilage repair carrier, matched surgical instrument set and cartilage repair system - Google Patents

Abstract

The operational tool group and cartilage repair systems that the present invention discloses a kind of repair of cartilage carrier, is arranged in pairs or groups.Repair of cartilage carrier includes ontology and multiple positioning columns.Ontology is for cellular structure and to carry cartilage repair material.Positioning column is fixed to ontology and the os osseum to be inserted into patient.Operational tool group includes locating sleeve and push type instrument.Locating sleeve has connected pathways.There is the first location structure on the side wall of connected pathways.Push type instrument includes outer tube and push rod.There is the second location structure mutually aligned with the first location structure on the outer wall of outer tube.Connected pathways of the outer tube to pass through locating sleeve.Push rod is slidably disposed in outer tube.One end of outer tube has moulding knife, to cut out area to be implanted on the affected part of patient, wherein the shape in area to be implanted corresponds to the shape of ontology.

Description

Cartilage repair carrier, matched surgical instrument set and cartilage repair system

technical field

The present invention relates to a carrier, an appliance set and a system, and in particular to a cartilage repair carrier, a matched surgical appliance set and a cartilage repair system.

Background technique

The articular cartilage tissue is located on the hard bone surface of the articular surface. It is a multi-functional and quite special tissue in human tissue. Its main function is to transmit the stress in different directions on the upper and lower hard bones on the articular surface, absorb the impact force of the hard bone layer to transmit the articular surface, and provide The lubricated joint surfaces with low friction coefficient and the coordination of muscle and ligament tissue can produce different motion patterns of sliding and rolling during joint activities.

The self-repair and regeneration ability of cartilage tissue is very insufficient, and once damaged, it often cannot recover on its own. According to statistics, more than 200,000 cases of artificial joint replacement surgery are required in the United States every year due to deep cartilage damage, and the number of cases is still increasing year by year. However, the replacement of an artificial joint requires the removal of a large amount of cartilage and hard bone tissue on the articular surface of the patient, which is quite damaging and destructive. After the artificial joint made of metal is implanted into the body, it can only maintain its function for about ten to fifteen years. For young patients, they will face the pain of re-artificial joint replacement surgery, while for elderly patients, they are often unable to bear the surgery of re-artificial joint replacement. Furthermore, repeated replacement of artificial joints is likely to lead to disability and poor mobility, which in turn creates a heavy burden on society and families.

The current medical treatment for damaged cartilage mainly adopts cartilage transplantation to repair the damaged cartilage. This method is a novel medical method invented in the past two decades, including autologous cartilage transplantation (ACI, autologous chondrocyte implantation) and osteoarticular allograft (OA, osteoarticular allograft). Replace the permanent damage of the artificial joint, and also do not need to periodically re-operate to replace the aging metal or plastic components. Among the above treatment methods, autologous cartilage transplantation is widely accepted, because the source of the graft is the patient's own tissue, and the problem of immune rejection of allograft or xenograft will not occur.

The mainstream surgical method of autologous transplantation is the "Mosaic plasty procedure", which was proposed by a Hungarian surgeon in 1995. A cylindrical plug of hard bone with cartilage and underlying contact was taken, and a filling groove of the same diameter was drilled with the same trephine at the damaged site, and then the undamaged healthy autologous soft and hard bone plug was filled. However, when repairing cartilage wounds by the mosaic method, the cartilage surface between the implanted cartilage plugs has a tile-like interface, and the chondrocytes between the interfaces are covered in a large amount of matrix, and their ability to divide is limited. Pretty bad. Therefore, in fact, it is not easy to form regenerative fusion between cartilage plugs; on the other hand, fibrocartilage tissue will be formed in the surrounding space of cartilage plugs, which makes the wounded surface prone to degenerative arthritis in the future.

At present, the repair of cartilage damage by tissue engineering in vitro culture of cartilage tissue also has the same problem, and after the cartilage tissue cultured in vitro is implanted into the cartilage wound, the problem of gap fusion between old and new cartilage tissue is still a difficult problem that cannot be broken through. Therefore, how to overcome the tissue regeneration and fusion between the transplanted cartilage (implant) and the original cartilage tissue (host) is a very important and urgent clinical research topic in orthopaedics.

SUMMARY OF THE INVENTION

The present invention provides a cartilage repair carrier, a matched surgical tool set and a cartilage repair system, which can improve the problems existing in the existing cartilage repair operation.

A cartilage repair carrier according to an embodiment of the present invention includes a body and a plurality of positioning posts. The body is a porous structure and is used to carry the cartilage repair material. One end of each positioning post is fixed to the body, and the other end is used for insertion into a patient's hard bone.

A surgical tool set according to an embodiment of the present invention includes a positioning sleeve and a pressing tool. The positioning sleeve has a through channel. A first positioning structure is provided on the side wall of the through channel. The pressing device includes an outer sleeve and a push rod. The outer wall of the outer sleeve has a second positioning structure. The outer sleeve is used to pass through the through channel of the positioning sleeve. The second positioning structure is aligned with the first positioning structure. The push rod is slidably arranged in the outer sleeve. One end of the outer sleeve is provided with a shaping knife, which is used to cut out an area to be implanted on the affected part of a patient.

A cartilage repair system according to an embodiment of the present invention includes a cartilage repair carrier, a positioning sleeve and a pressing instrument. The cartilage repair carrier includes a body and a plurality of positioning posts. The body is a porous structure and is used to carry the cartilage repair material. One end of each positioning post is fixed to the body, and the other end is used for insertion into a patient's hard bone. The positioning sleeve has a through channel. A first positioning structure is provided on the side wall of the through channel. The pressing device includes an outer sleeve and a push rod. The outer wall of the outer sleeve has a second positioning structure. The outer sleeve is used to pass through the through channel of the positioning sleeve. The second positioning structure is aligned with the first positioning structure. The push rod is slidably arranged in the outer sleeve. One end of the outer sleeve is provided with a shaping knife, which is used to cut out an area to be implanted on the affected part of the patient. The shape of the area to be implanted corresponds to the shape of the body.

Based on the above, in the cartilage repair carrier, the matched surgical tool set and the cartilage repair system of the present invention, the cartilage repair carrier with a porous structure facilitates the gap fusion between new and old cartilage tissues, and the surgical tool set makes the cartilage repair carrier Can be easily implanted into the patient's affected area.

In order to make the above-mentioned features and advantages of the present invention more obvious and easy to understand, the following embodiments are given and described in detail with the accompanying drawings as follows.

Description of drawings

1 is a schematic diagram of a cartilage repair carrier according to an embodiment of the present invention;

2 is a solid photograph of a cartilage repair carrier according to an embodiment of the present invention;

3 is a schematic diagram of a positioning sleeve of a surgical tool set according to an embodiment of the present invention;

FIG. 4A and FIG. 4B are a schematic external view and a schematic cross-sectional view of a pressing device of a surgical tool set according to an embodiment of the present invention, respectively;

5 is a schematic diagram of a debridement drill of a surgical tool set according to an embodiment of the present invention;

6 is a schematic diagram of a drilling instrument of a surgical instrument set according to an embodiment of the present invention;

7A to 7G are photographic views of various stages of cartilage repair surgery using the cartilage repair system according to an embodiment of the present invention;

FIG. 8 is a schematic diagram of the usage mode of the cartilage repair carrier according to an embodiment of the present invention;

9A and FIG. 9B are photographs of the affected part after 6 months of recovery of an experimental example using the cartilage repair system according to an embodiment of the present invention for surgery and a control example for surgery using traditional surgical instruments;

10A and FIG. 10B are respectively the X-ray pictures of the affected part after 6 months of recovery for an experimental example using the cartilage repair system according to an embodiment of the present invention for surgery and a control example for using traditional surgical instruments for surgery;

11A and FIG. 11B are respectively photographs of the affected part samples after 6 months of recovery from an experimental example using the cartilage repair system according to an embodiment of the present invention and a control example using traditional surgical instruments for surgery;

12A and FIG. 12B are respectively a photo of an affected part sample after 12 months of recovery of an experimental example using the cartilage repair system according to an embodiment of the present invention and a control example using traditional surgical instruments for surgery;

13A and FIG. 13B are respectively the stained section views of the affected part after 12 months of recovery in an experimental example using the cartilage repair system according to an embodiment of the present invention and a control example using traditional surgical instruments for surgery;

FIG. 14 is a quantification diagram of the wound width of an experimental example performed using the cartilage repair system according to an embodiment of the present invention and a control example performed using a conventional surgical tool.

Symbol Description

100: Cartilage Repair Carrier

110: Ontology

112: Positioning angle

114: Groove

120, 510: positioning column

200: Positioning sleeve

210: First positioning structure

220: casing positioning column

230: Grip

T10: Through the channel

W10: Sidewall

P10: Discharge opening

300: Press-type instruments

310: Outer casing

312: Second positioning structure

314: Shaper Knife

314A: Positioning Angle

320: Putter

330: Elastic recovery piece

W20: outer wall

400: Debridement Drill

410: Stopper

500: Drilling Instruments

520: Third positioning structure

A10: Area to be implanted

P20: Positioning hole

Detailed ways

A surgical tool set according to an embodiment of the present invention includes a positioning sleeve and a pressing tool. In addition, the cartilage repair system according to an embodiment of the present invention further includes a cartilage repair carrier in addition to the above-mentioned surgical tool set.

FIG. 1 is a schematic diagram of a cartilage repair carrier according to an embodiment of the present invention, and FIG. 2 is a solid photo of the cartilage repair carrier according to an embodiment of the present invention. Please refer to FIG. 1 and FIG. 2 simultaneously. In one embodiment, the cartilage repair carrier 100 includes a body 110 and a plurality of positioning posts 120 . The body 110 is a porous structure for carrying the cartilage repair material. The positioning posts 120 are solid structures. One end of each positioning post 120 is fixed to the body 110 , and the other end is used to insert into the patient's hard bone during the operation, so as to fix the entire cartilage repair carrier 100 on the patient's hard bone. Optionally, the body 110 of this embodiment may further have a groove 114 , and the space of the groove 114 is used to carry the material to be used for cartilage repair.

In one embodiment, the body 110 of the cartilage repair carrier 100 adopts a porous structure, so that adjacent tissues or cells and lubricating fluid can enter the interior of the body 110 or the space of the groove 114 through the aperture, and repair together with the cartilage repair material. The affected area, thereby enhancing the recovery effect.

In one embodiment, the material of the body 110 may be a biodegradable material. Therefore, after the repair of the affected part of the patient is completed, the cartilage repair carrier 100 can be naturally decomposed and metabolized in the body, leaving only the normal cartilage with restored function, without leaving any non-human body natural products in the body. In one embodiment, a single biodegradable material can be used as the material of the body 110 , and the composition of the material is relatively simple, which can further shorten the time required for complete degradation. In one embodiment, the material of the body 110 can be a single polymer material, such as polylactic acid (PLA), polyglycolic acid (PGA), polycaprolactone (PCL), polyvinyl alcohol (Polyvinylalcohol, PVA), polyhydroxy-alkanoates (polyhydroxy-alkanoates, PHA), but not limited thereto. In another embodiment, the material of the body 110 may be a composite polymer material, such as a co-polymer of at least two of the above polymers, but not limited thereto.

In one embodiment, the material of the positioning post 120 can be a biodegradable material. In one embodiment, a single biodegradable material can be used as the material of the positioning post 120 , and the composition of the material is relatively simple, which can further shorten the time required for complete degradation. In one embodiment, the material of the positioning column 120 can be a single polymer material, such as polylactic acid (PLA), polyglycolic acid (PGA), polycaprolactone (PCL), polyethylene Alcohol (Polyvinyl alcohol, PVA), polyhydroxy-alkanoates (polyhydroxy-alkanoates, PHA), but not limited thereto. In another embodiment, the material of the positioning post 120 may be a composite polymer material, such as a co-polymer of at least two of the above polymers, but not limited thereto. The material of the body 110 and the material of the positioning post 120 may be the same or different.

In one embodiment, the porosity of the porous structure of the body 110 is 50% to 90%, for example, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90% . In addition, the pore size of the porous structure is about 50 μm to 1,000 μm. In one embodiment, the appearance of the main body 110 may be a disc shape or a cover shape, but is not limited thereto. In one embodiment, the diameter of the body 110 may be about 8 mm to 10 mm, which may be suitable for different degrees of cartilage defects. The wall thickness of the body 110 may be 1 mm to 1.5 mm, with sufficient strength to resist the compressive force of the surrounding tissue, and not to be compressed into the storage space of the cartilage repair material. The total height of the body 110 can be 1.8mm to 2.2mm, which is approximately the same as the thickness of the cartilage layer of the human knee joint, so that the hard bone layer will not be violated during the operation, and will not protrude from the surface of the knee joint after the operation, which can avoid Worn by articular surfaces. In one embodiment, the positioning post 120 may be 1 mm to 2 mm in diameter, 2 mm to 3 mm in length, and may be a solid post in order to secure the body 110 to the damaged cartilage with minimal disruption to a pair of hard bones. place.

In one embodiment, the periphery of the body 110 may have a positioning angle 112 . The positioning angle 112 may be a protruding small point of about 0.5 mm to 1 mm, such as a protruding triangular shape, but not limited thereto. The positioning angle 112 can be matched with positioning structures on other surgical instruments to assist positioning during the operation and guide the cartilage repair carrier 100 to the correct position.

3 is a schematic diagram of a positioning sleeve of a surgical tool set according to an embodiment of the present invention. Please refer to FIG. 3 , the positioning sleeve 200 has a through channel T10 . A first positioning structure 210 is formed on the side wall W10 of the through channel T10. 4A and FIG. 4B are a schematic external view and a schematic cross-sectional view of a push-type instrument of a surgical instrument set according to an embodiment of the present invention, respectively. Referring to FIGS. 4A and 4B , the pressing device 300 includes an outer sleeve 310 and a push rod 320 . A second positioning structure 312 is formed on the outer wall W20 of the outer sleeve 310 . The outer sleeve 310 is used to pass through the through channel T10 of the positioning sleeve 200 of FIG. 3 . The second positioning structure 312 and the first positioning structure 210 in FIG. 3 can be aligned with each other, so as to ensure that the positioning sleeve 200 and the pressing device 300 can be accurately positioned during the operation. The push rod 320 is disposed in the outer sleeve 310 in a slidable form, and can slide in the outer sleeve 310 by pressing or pushing. One end of the outer sleeve 310 has a shaping knife 314 for cutting out an area to be implanted (not shown) on the affected part of the patient. The shape of the area to be implanted may correspond to the shape of the body 110 of FIG. 1 .

Referring to FIGS. 3 , 4A and 4B , when the outer sleeve 310 of the pressing device 300 passes through the through channel T10 of the positioning sleeve 200 , because the positioning sleeve 200 is provided with the first positioning structure 210 , the pressing device 300 The outer wall W20 of the outer sleeve 310 is provided with a second positioning structure 312, and the second positioning structure 312 must be aligned with the first positioning structure 210, so that the sliding position of the pressing device 300 can be restricted. In addition, the slidable push rod 320 disposed in the outer sleeve 310 protrudes out of the outer sleeve 310 at one end away from the shaping knife 314 . When the user pushes the end of the push rod 320 protruding from the outer sleeve 310 , the push rod 320 can be pushed toward the direction of the shaping knife 314 .

In one embodiment, the shape of the shaping blade 314 can be designed to match the shape of the body 110 of the cartilage repair carrier 100 , that is, the shaping blade 314 is designed to be consistent with the contour and configuration of the body 110 so as to be completely accommodated. The body 110 allows the cartilage repair carrier 100 to be completely placed in the area constructed by the shaping knife 314 . In this way, the user only needs to push the push rod 320 to push the cartilage repair carrier 100 into the target of the affected part. In other words, the pressing instrument 300 can be used as a tool for pushing the cartilage repair carrier 100 to the to-be-implanted area in addition to the shaping knife 314 to cut out the to-be-implanted area. In another embodiment, for the convenience of use, an elastic restoring member 330, such as a spring, can be arranged between the outer sleeve 310 and the push rod 320, so that the push rod 320 can be automatically reset.

Referring to FIG. 3 , in one embodiment, one end of the positioning sleeve 200 has a plurality of sleeve positioning posts 220 for inserting into the affected part of the patient, so that the position of the positioning sleeve 200 can be preliminarily fixed and framed during the operation. Surgical site for subsequent precise surgery. The length of the casing positioning column 220 may be 8-12 mm, for example, 10 mm, and the angle between the outer surface of the casing positioning column 220 and its central axis may be 0.5-2 degrees, such as 1 degree, so that the casing positioning column When the 220 is driven into the bone for fixation, the resistance can be reduced and it can be more easily driven into the bone. The diameter of the through channel T10 may be 12-14 mm, for example, 13 mm, which may provide space for subsequent operation of surgical instruments. In addition, at least one discharge opening P10 is provided through the side wall W10 of the passage T10, for example, two or three, but not limited thereto. In one embodiment, four discharge openings P10 may be provided. The discharge opening P10 may be an elongated opening with a width of 1 to 5 mm and a length of 20 to 30 mm, for example, an elongated opening with a width of 2.5 mm and a length of 24 to 25 mm. When the debridement drill is used for subsequent debridement, the removed tissue can be discharged through the discharge opening P10 to leave a clean wound.

In addition, in yet another embodiment, the other end of the positioning sleeve 200 opposite to the sleeve positioning post 220 may have a handle 230 to provide a user to hold, so as to improve the convenience of use. And, the above-mentioned first positioning structure 210 disposed on the side wall W10 of the through channel T10 can be a guide channel with a length of about 0.5-2 mm, for example, 1 mm, to provide the function of positioning other surgical instruments to ensure the above-mentioned cartilage The plurality of positioning posts 120 of the repair carrier 100 can be smoothly inserted and tightly pressed into the small holes on the hard bone.

Please refer to FIG. 1 and FIG. 4A at the same time, in one embodiment, the shaping knife 314 is provided with a positioning angle 314A. The positioning angle 314A and the positioning angle 112 of the cartilage repair carrier 100 can cooperate with each other to perform alignment. Therefore, when the cartilage repair carrier 100 is placed in the area encircled by the shaping knife 314, it can be ensured that the orientation of the cartilage repair carrier 100 is in the orientation desired for subsequent operations.

5 is a schematic diagram of a debridement drill of a surgical tool set according to an embodiment of the present invention. Please refer to FIG. 3 and FIG. 5 at the same time. In one embodiment, the debridement drill bit 400 can pass through the through channel T10 of the positioning sleeve 200 to remove the cartilage in the patient's area to be implanted, so as to vacate the patient's affected area. For implantation in the position of the cartilage repair carrier 100 as shown in FIG. 1 . In another embodiment, the debridement drill bit 400 has a stop portion 410 . The size of the stopper portion 410 is larger than that of the through passage T10. In one embodiment, the diameter of the stopper portion 410 is about 14-16 mm, for example, 15 mm. Therefore, when the debridement bit 400 passes through the through channel T10 of the positioning sleeve 200 , the stopper 410 prevents the debridement bit 400 from moving forward continuously, thereby limiting the debridement depth of the debridement bit 400 . For example, if the stop portion 410 makes the portion of the debridement drill 400 protruding from the positioning sleeve 200 by 2 mm, the depth of the cartilage cut by the debridement drill 400 will also be 2 mm. In other words, the depth of the cartilage excised by the debridement drill 400 is matched to the height of the body 110 of the cartilage repair carrier 100 .

In another embodiment, the diameter of the drilled portion under the debridement drill bit 400 is about 8 to 10 mm, which is matched with the diameter of the body 110 of the cartilage repair carrier 100 . In more detail, the diameter of the drilled portion under the debridement drill 400 is almost equal to the outer diameter of the body 110 of the cartilage repair carrier 100 , so as to clear a space suitable for accommodating the cartilage repair carrier 100 . Since the debridement drill bit 400 rotates in the through channel T10, the debridement drill bit 400 does not have a structure corresponding to the first positioning structure 210 of the through channel T10.

6 is a schematic diagram of a drilling instrument of a surgical instrument set according to an embodiment of the present invention. 3 and 6 at the same time, the drilling instrument 500 of this embodiment is used to drill a plurality of positioning holes (not shown) in the hard bone of the patient to be implanted through the through channel T10 of the positioning sleeve 200 Show). For example, the drilling instrument 500 has a plurality of positioning posts 510 , and the positions of the positioning holes drilled by the positioning posts 510 may correspond to the positions of the positioning posts 120 of the cartilage repair carrier 100 in FIG. 1 . For example, if the number of the positioning columns 120 is three, the azimuth angles of the three positioning columns 120 relative to the central axis of the drilling instrument 500 are 0 degrees, 120 degrees and 240 degrees, that is, the three positioning columns 120 are Divide in a point-symmetric fashion. The included angle between the outer surface of the positioning post 120 and its central axis is, for example, 3 degrees to 5 degrees, so as to drill a positioning hole with a slope. Specifically, because the positioning hole has a slope, the deeper the positioning post 120 of the cartilage repair carrier 100 is in the positioning hole, the more tightly the positioning hole can grasp the positioning post 120 to firmly fix the cartilage repair carrier 100 on the patient's body. affected part.

In addition, in another embodiment, the drilling instrument 500 has, for example, a third positioning structure 520, and the third positioning structure 520 is aligned with the first positioning structure 210 in FIG. 3 . Therefore, the orientation of the drilling instrument 500 when sliding in the outer sleeve 310 is limited, thereby ensuring that the position of the positioning hole drilled by the drilling instrument 500 corresponds to the position of the positioning post 120 of the subsequently implanted cartilage repair carrier 100 .

7A to 7G are photographs of various stages of cartilage repair surgery performed on pigs using the cartilage repair system according to an embodiment of the present invention. Please refer to FIG. 7A first, a general adult pig is selected as the object for establishing minimally invasive knee joint surgery. The number of pigs, approximate age, body weight, sex, and relevant information on surgery and autopsy were recorded during the experiment. Before the operation, the pigs only needed to fast for 24 hours. The anesthetic used in the operation was 3% sodium pentobarbital, which was injected from the hind leg at a dose of about 1 mL/kg, and the dose was increased or decreased according to the anesthesia of each pig. After confirming anesthesia, the pigs were shaved and disinfected with iodine, and a sterile towel was used to cover the area outside the surgical site, and only the joints were exposed to ensure that the operation was performed in a sterile state. Next, a wound of about 3 cm is incised on the outer skin, and the soft tissue is peeled off to expose the position where the cartilage repair carrier 100 as shown in FIG. 1 is to be implanted.

Next, please refer to FIG. 7B , place the positioning sleeve 200 shown in FIG. 3 on the affected part, and then drive the two sleeve positioning posts 220 of the positioning sleeve 200 into the affected part and fix it. Next, referring to FIG. 7C , use the pressing device 300 shown in FIG. 4A to insert the positioning sleeve 200 and use a shaping knife to cut out the area A10 to be implanted in the affected part. Next, the debridement drill 400 shown in FIG. 5 is used to insert the positioning sleeve 200 to remove the cartilage tissue in the area A10 to be implanted.

7D, insert the drilling instrument 500 shown in FIG. 6 into the positioning sleeve 200, and drill a plurality of positioning holes P20 shown in FIG. 7E in the hard bone in the area A10 to be implanted. Next, referring to FIG. 7F , the cartilage repair carrier 100 shown in FIG. 1 is placed in the area A10 to be implanted. For the convenience of understanding, in FIG. 7F , the cartilage repair carrier 100 is placed beside the area A10 to be implanted, but in actual operation, the pressing instrument 300 as shown in FIG. 4A is used to directly push the cartilage repair carrier 100 into the area to be implanted District A10. Since the shape of the shaping knife corresponds to the shape of the cartilage repair carrier 100 , and the drilling instrument 500 and the positioning sleeve 200 also have positioning structures that are aligned with each other, the position of the positioning hole P20 drilled by the drilling instrument 500 will be different. Directly corresponding to the positioning column 120 of the cartilage repair carrier 100 , no additional alignment step is required, which not only shortens the time required for the operation, but also improves the success rate of the operation due to the precise alignment. FIG. 7G shows that the cartilage repair carrier 100 has been placed in the area A10 to be implanted.

FIG. 8 is a schematic diagram of the usage of the cartilage repair carrier according to an embodiment of the present invention. Please refer to FIG. 8 , the cartilage repair material is obtained from the patient itself, especially from the cartilage at the unstressed joint of the joint, for example, the autologous cartilage is scraped with a curette. The autologous cartilage taken out above was placed in a 10 cm petri dish, and the cartilage was minced with a scalpel. The size of the fragments was controlled between 560-800 μm with a 20-hole to 40-hole mesh screen. The minced cartilage fragments were collected in a 15 ml centrifuge tube, 5 ml of collagenase was added, and placed in a 37° C. incubator for 1 hour to free some chondrocytes. Collagenase was placed in phosphate-buffered saline (PBS), and its ratio was adjusted to 2 mg/ml PBS. The dissolved cartilage block was separated with collagenase, placed in a centrifuge, and centrifuged at 1500 r.p.m. for 5 minutes to separate collagenase from the cartilage block. The cartilage fragments after centrifugation, after suctioning off the clarified collagenase supernatant (supernatant), the remaining cartilage fragments and cell tissue were washed twice with PBS and centrifuged twice to remove the residual collagenase to complete the cartilage. Preparation of restorative materials.

Next, the above-prepared cartilage repair material is placed into a 1c.c. injection syringe, and an 18-gauge needle (18G) is used to inject the prepared cartilage repair material onto the cartilage repair carrier 100 . After that, the cartilage repair carrier 100 is placed with the side bearing the cartilage repair material facing the affected part, so that the cartilage repair material directly contacts the affected part. Since the cartilage repair material has a certain viscosity, it is not easy to fall off the cartilage repair carrier.

9A and 9B are respectively pictures of the restored affected part of an experimental example using the cartilage repair system according to an embodiment of the present invention and a control example using traditional surgical tools for surgery. As can be seen from FIG. 9A , 12 months after the operation using the surgical tool set of an embodiment of the present invention, the cartilage of the affected part (as indicated by the arrow) recovered well, and almost no wound traces were seen. On the contrary, for those who underwent surgery with the traditional surgical instrument group, the cartilage in the affected part (indicated by the arrow in Figure 9B) after 12 months was in a poor state of recovery, and the wound marks were obvious.

FIG. 10A and FIG. 10B are respectively the X-ray films of the affected part after six months of recovery of an experimental example using the cartilage repair system according to an embodiment of the present invention and a control example using traditional surgical tools for surgery. pigs. As can be seen from FIG. 10A , 6 months after the operation using the surgical instrument set according to an embodiment of the present invention, the cartilage in the affected part recovered rapidly, and the wound depth was only 2.8 mm. On the contrary, in those who performed the operation with the traditional surgical instrument group, the recovery of the cartilage in the affected part was slow after 6 months, as shown in Fig. 10B , and the depth of the wound was 13.4 mm.

11A and FIG. 11B are respectively pictures of the affected part samples after 6 months of recovery from an experimental example using the cartilage repair system according to an embodiment of the present invention and a control example using traditional surgical tools for surgery. pig. It can be seen from FIG. 11A , 6 months after the operation using the surgical tool set of an embodiment of the present invention, the hard bone of the affected part (as indicated by the arrow) recovered rapidly, with only a few surgical residues, and the cartilage recovered well. On the contrary, for those who performed surgery with the traditional surgical instrument group, the recovery of the hard bone in the affected part (indicated by the arrow in Figure 11B ) was slow after 6 months, and there were many surgical residues.

12A and FIG. 12B are respectively photos of the affected part samples after 12 months of recovery from an experimental example using the cartilage repair system according to an embodiment of the present invention and a control example using traditional surgical tools for surgery. The surgical objects are all as described above. pig. As can be seen from FIG. 12A , 12 months after the operation using the surgical instrument set of an embodiment of the present invention, the hard bone of the affected part (as indicated by the arrow) recovered well, with almost no surgical residue, and the new hard bone tissue has indeed grown. enter. On the contrary, for those who operated with the traditional surgical instrument group, the recovery of the hard bone in the affected part (indicated by the arrow in Fig. 12B ) was poor after 12 months, and there were still many surgical residues.

13A and FIG. 13B are respectively the restored stained sections of the affected part of an experimental example using the cartilage repair system according to an embodiment of the present invention and a control example using traditional surgical tools for surgery, and the surgical objects are all pigs as described above. . Fig. 13A and Fig. 13B are stained with Safranin-O and Fast Green FCF at the same time to further examine the condition of cartilage hyperplasia and the condition of hard bone recovery.

Please refer to FIG. 13A and FIG. 13B at the same time. It can be seen from the stained area of safranin that 12 months after the operation using the surgical tool set according to an embodiment of the present invention, as indicated by the upper arrow in FIG. 13A , the cartilage hyperplasia is in good condition. , while those who performed surgery using the traditional surgical tool set, as indicated by the upper arrow in Figure 13B, had normal cartilage hyperplasia. On the other hand, it can be seen from the stained area of fast green that 12 months after the operation using the surgical tool set according to an embodiment of the present invention, as indicated by the middle arrow in FIG. For those who underwent surgery in the appliance group, as indicated by the middle arrow in Fig. 13B, there was almost no hyperplasia of hard bone.

FIG. 14 is a quantification diagram of the wound width of an experimental example performed using the cartilage repair system according to an embodiment of the present invention and a control example performed using a conventional surgical tool. Referring to FIG. 14 , the wound width of the experimental example using the surgical instrument set according to an embodiment of the present invention in the second half of the operation is only 60% of the wound width of the control example using the traditional surgical instrument in the second half of the operation, and The wound width of the experimental example at one year after the operation was about 50% of the wound width of the control example at one year after the operation.

To sum up, in the cartilage repair carrier, the matched surgical tool set and the cartilage repair system of the present invention, the cartilage repair carrier adopts a porous structure, which is helpful for the gap fusion between new and old cartilage tissues, and the surgical tool set makes Minimally invasive cartilage repair surgery possible. In addition, when the cartilage repair carrier is made of biodegradable materials, it can carry substances that promote cartilage repair. With a positionable surgical tool set, the cartilage repair carrier can be accurately positioned and implanted into the affected part of the patient in a minimally invasive manner. In this way, the cartilage defect site can be reconstructed with less damage to the hard bone, and the cartilage repair carrier can be naturally decomposed and metabolized in the body after the repair is completed.

Although the present invention is disclosed in conjunction with the above embodiments, it is not intended to limit the present invention. Anyone with ordinary knowledge in the technical field can make some changes and modifications without departing from the spirit and scope of the present invention. Therefore, The scope of protection of the present invention should be defined by the appended claims.

Claims (15)

1. a cartilage repair carrier, is characterized in that, comprises: a body, which is a porous structure for carrying the cartilage repair material; and A plurality of positioning posts, one end of each of the positioning posts is fixed to the body, and the other end is used for inserting into the patient's hard bone. 2. The cartilage repair carrier according to claim 1, wherein the material of the body is a biodegradable material. 3. The cartilage repair carrier according to claim 1, wherein the material of the body is polylactic acid, polyglycolic acid, polycaprolactone, polyvinyl alcohol, polyhydroxyalkanoic acid or a copolymer of at least two of the above polymers. 4. The cartilage repair carrier according to claim 1, wherein the porosity of the porous structure is 50%-90%. 5. The cartilage repair carrier of claim 1, wherein the periphery of the body has a positioning angle. 6. A set of surgical tools, characterized in that, comprising: a positioning sleeve having a through channel, wherein a first positioning structure is provided on the side wall of the through channel; and A pressing device includes an outer sleeve and a push rod, wherein the outer wall of the outer sleeve has a second positioning structure, the outer sleeve is used to pass through the through channel of the positioning sleeve, and the second positioning structure and the first positioning structure The structures are aligned with each other, the push rod is slidably arranged in the outer sleeve, and one end of the outer sleeve is provided with a shaping knife, which is used to cut out the area to be implanted on the affected part of the patient. 7 . The surgical tool set of claim 6 , wherein one end of the positioning sleeve has a plurality of sleeve positioning posts for inserting into the affected part of the patient. 8 . 8. The surgical tool set as claimed in claim 7, wherein the other end of the positioning sleeve has a handle for the user to hold. 9 . The surgical tool set of claim 6 , wherein a side wall of the through channel is further provided with a discharge opening. 10 . 10. The surgical tool set of claim 6, wherein the shaping knife has a positioning angle. 11. The surgical tool set of claim 6, further comprising a debridement drill for removing cartilage in the to-be-implanted region of the patient through the through-channel of the positioning sleeve. 12 . The surgical tool set of claim 11 , wherein the debridement drill has a stopper, and the stopper has a size larger than that of the through channel for limiting the debridement depth of the debridement drill. 13 . 13. The surgical tool set of claim 6, further comprising a drilling instrument for drilling a plurality of locations through the through channel of the positioning sleeve to drill a plurality of locations on the hard bone at the to-be-implanted region of the patient hole. 14 . The surgical tool set of claim 13 , wherein the drilling instrument has a third positioning structure, and the third positioning structure and the first positioning structure are mutually aligned. 15 . 15. A cartilage repair system, comprising: A cartilage repair carrier, comprising a body and a plurality of positioning columns, wherein the body is a porous structure and used to carry cartilage repair materials, one end of each positioning column is fixed to the body, and the other end of each positioning column is used to insert the patient's Bone; a positioning sleeve having a through channel, wherein a first positioning structure is provided on the side wall of the through channel; and A pressing device includes an outer sleeve and a push rod, wherein the outer wall of the outer sleeve has a second positioning structure, the outer sleeve is used to pass through the through channel of the positioning sleeve, and the second positioning structure and the first positioning structure The structures are aligned with each other, the push rod is slidably arranged in the outer sleeve, and one end of the outer sleeve is provided with a shaping knife to cut out the to-be-implanted area on the affected part of the patient, and the shape of the to-be-implanted area matches the should be the shape of the body.