CN109943466A - A cell crawler - Google Patents

Abstract

The invention discloses a kind of cell climbing sheet grabbers, including hollow cylinder, push rod and cell climbing sheet, hollow cylinder is internally connected with push rod, and push rod and hollow cylinder dismantling connection, the bottom end of hollow cylinder is fixed with sucker, surface a side of hollow cylinder offers high-order limit hole and low level limit hole, top a side of hollow cylinder is connected with by pressure arm, the interior bottom portion of hollow cylinder is fixed with reset spring, the bottom end of push rod is fixed with rubber plug compatible with hollow cylinder, the bottom side side of push rod is connected with elastic spacing device, and elastic spacing device is connect by tilting bar with push rod, the diameter of elastic spacing device is adapted with the diameter of high-order limit hole and low level limit hole.The utility model has the advantages that cell climbing sheet grabber of the invention can easily, quickly grab cell climbing sheet, because of the probability that misoperation causes creep plate broken when reducing crawl creep plate, provide convenience to carry out related experiment.

Description

A cell crawler

technical field

The invention relates to the technical field of equipment for transferring and separating cell climbing sheets, in particular to a cell climbing sheet grabber.

Background technique

The cell climbing technique refers to immersing the glass slide in the cell culture medium and growing the cells on the glass slide. It is mainly used for histology, immunohistochemistry, in situ hybridization, etc. The glass slides used in this technique are also called cell climbing slides. Generally, the surface is specially treated, which is more suitable for the adhesion of adherent cells. The thickness of the slide is 0.17mm, and the diameter is 8mm/14mm/20mm/25mm. It is suitable for 48-well cell culture plate/24-well cell culture plate/12-well cell culture plate/6-well cell culture plate respectively. The traditional operating steps are:

1. Sterilize the cell slides.

2. Drop 1 drop of culture medium into each well of the culture plate, then place the climbing sheet on the drop and press it tightly (the climbing sheet is adsorbed on the culture plate by the action of surface tension).

3. Routine cell digestion, centrifugation, and resuspension. Add the pipetted cell suspension to each slide respectively, or directly inoculate cells into the culture wells as usual to allow them to grow adherently.

4. Aspirate the cell culture medium, wash three times with PBS, and fix with 4% paraformaldehyde or other fixatives.

5. After completing the cell slides, carefully remove the slides with tweezers for follow-up testing.

Among them, the climbing pieces attached to the wall are difficult to take out with conventional small tweezers, and the climbing pieces are easy to be crushed when they are taken out, which is also the biggest defect of this method. At present, there is a lack of simple and safe methods or instruments for grasping and crawling films. For the problems in the related art, no effective solutions have been proposed yet.

SUMMARY OF THE INVENTION

The technical task of the present invention is to solve the above problems by providing a cell crawler grasper to solve the above problems.

The technical scheme of the present invention is realized as follows:

A cell-climbing sheet grabber, comprising a hollow cylinder, a push rod and a cell-climbing sheet, the push rod is connected inside the hollow cylinder, and the push rod is disassembled and connected to the hollow cylinder, so the The diameter of the bottom end of the hollow cylindrical body is smaller than the diameter of the top end of the hollow cylindrical body, and the bottom end of the hollow cylindrical body is a conical structure, the bottom end of the hollow cylindrical body is fixed with a suction cup, and the hollow cylindrical body is A high-position limit hole and a low-position limit hole are opened on one side of the surface of the hollow cylinder, the top side of the hollow cylinder is connected with a pressing arm, the inner bottom of the hollow cylinder is fixed with a return spring, and the push rod is The bottom end is fixed with a rubber stopper that is adapted to the hollow cylinder, and an elastic stopper is connected to one side of the bottom of the push rod, and the elastic stopper is connected to the push rod through an inclined rod, The diameter of the elastic stopper is adapted to the diameters of the high limit hole and the low limit hole.

Further, the high limit hole, the low limit hole and the pressing arm are located on the same side of the hollow cylinder and on the same horizontal line.

Further, the rubber plug is in an interference fit with the hollow cylinder, and the diameter of the bottom end of the rubber plug is smaller than the diameter of the top end of the rubber plug.

Further, a pressing plate is fixed on the top end of the push rod, and the pressing plate is a circular pressing plate.

Further, the vertical height of the pressing arm is on the same horizontal plane as the low limit hole, and a convex pressing surface is fixed at the bottom end of the pressing arm and close to one side of the hollow cylinder.

Further, the suction cup is made of rubber.

Beneficial effects of the present invention:

1. The cell-climbing slide grasper of the present invention can easily and quickly grasp the cell-climbing slides, reduce the probability of the slide-climbing slides being broken due to improper operation when grasping the slide-climbing slides, and provide convenience for carrying out related experiments.

2. The present invention is simple in structure, convenient in operation, and more convenient for people to use and grasp the cell crawling slides.

Description of drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the accompanying drawings required in the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some of the present invention. In the embodiments, for those of ordinary skill in the art, other drawings can also be obtained according to these drawings without any creative effort.

1 is a schematic diagram of a cell crawler grabber according to an embodiment of the present invention;

2 is a schematic structural diagram of a cell crawling sheet according to an embodiment of the present invention;

3 is a schematic structural diagram of a hollow cylinder according to an embodiment of the present invention;

FIG. 4 is a schematic structural diagram of a push rod according to an embodiment of the present invention.

In the picture:

1. Hollow cylinder; 2. Push rod; 3. Cell climbing sheet; 4. Suction cup; 5. High limit hole; 6. Low limit hole; 7. Pressing arm; 8. Return spring; 9. Rubber stopper; 10. Elastic limiter; 11. Tilt rod; 12. Pressing plate.

Detailed ways

In the description of the present invention, it should be understood that the terms "center", "lateral", "top", "bottom", "left", "right", "vertical", "horizontal", "top", The orientation or positional relationship indicated by "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying the indicated device. Or elements must have a particular orientation, be constructed and operate in a particular orientation, and therefore should not be construed as limiting the invention. In addition, the terms "first" and "second" are only used for descriptive purposes, and should not be construed as indicating or implying relative importance or implying the number of indicated technical features. Thus, a feature defined as "first" or "second" may expressly or implicitly include one or more of that feature. In the description of the present invention, unless otherwise specified, "plurality" means two or more. Additionally, the term "comprising" and any variations thereof are intended to cover non-exclusive inclusion.

The present invention will be further described below with reference to the accompanying drawings and specific embodiments.

Embodiment 1, as shown in FIGS. 1-4 , a cell crawler grasper according to an embodiment of the present invention includes a hollow cylinder 1 , a push rod 2 and a cell crawler 3 . The push rod 2 is connected, and the push rod 2 is disassembled and connected to the hollow cylinder 1, the diameter of the bottom end of the hollow cylinder 1 is smaller than the diameter of the top end of the hollow cylinder 1, and the hollow cylinder 1 is The bottom end of the cylinder body 1 has a conical structure, the bottom end of the hollow cylinder body 1 is fixed with a suction cup 4, and a high position limit hole 5 and a low position limit hole 6 are opened on one side of the surface of the hollow cylinder body 1, The top side of the hollow cylinder 1 is connected with a pressing arm 7 , the inner bottom of the hollow cylinder 1 is fixed with a return spring 8 , and the bottom end of the push rod 2 is fixed with the hollow cylinder 1 . An elastic stopper 10 is connected to one side of the bottom of the push rod 2, and the elastic stopper 10 is connected to the push rod 2 through an inclined rod 11, and the elastic limit The diameter of the device 10 is adapted to the diameters of the high limit hole 5 and the low limit hole 6 .

Embodiment 2, as shown in FIG. 2 , the high limit hole 5 , the low limit hole 6 and the pressing arm 7 are located on the same side of the hollow cylinder 1 and on the same horizontal line.

Embodiment 3, as shown in FIG. 1 , the rubber stopper 9 is in an interference fit with the hollow cylinder 1 , and the diameter of the bottom end of the rubber stopper 9 is smaller than the diameter of the top end of the rubber stopper 9 .

In the fourth embodiment, as shown in FIG. 1 or 3 , the top end of the push rod 2 is fixed with a pressing plate 12 , and the pressing plate 12 is a circular pressing plate 12 .

Embodiment 5, as shown in FIG. 1-2 , the vertical height of the pressing arm 7 is on the same horizontal plane as the low limit hole 6 , and the bottom end of the pressing arm 7 is close to the bottom of the hollow cylinder 1 . A raised pressing surface is fixed on one side.

Embodiment 6, as shown in Figures 1-2, the suction cup 4 is made of rubber.

Specific work flow: when using, first press the push rod 2 to make the elastic stopper 10 located in the low limit hole 6 , when the push rod 2 is pressed, the elastic stopper 10 is guided into the hollow cylinder 1 by the inclined rod 11 . When the elastic limiter 10 reaches the low limit hole 6 , it bounces up to fix the position of the push rod 2 . Then, place the suction cup 4 on a corner of the cell climbing sheet 3, and apply a slight downward force to make the suction cup 4 and the cell climbing sheet 3 in close contact. Then, the pressing arm 7 is pressed inward, and the pressing arm 7 pushes the elastic stopper 10 inward, so that the elastic stopper 10 is retracted into the hollow cylinder 1 . The push rod 2 is pushed by the return spring 8, and the return spring 8 is a compression spring, which drives the rubber plug 9 to move upward. Since the rubber plug 9 and the hollow cylinder 1 are in an interference fit, when the rubber plug 9 moves upward, a negative pressure is formed inside the hollow cylinder 1 . At this time, the suction cup 4 is in close contact with the cell climbing sheet 3 . Pull the cell crawler 3 gripper upward to separate the cell crawler 3 from the attachment surface. After moving the cell climbing sheet 3 to the desired position, press the push rod 2 according to the aforementioned method, so that the elastic stopper 10 reaches the low limit hole 6 . At this time, the negative pressure in the hollow cylinder 1 disappears, and the cell crawling sheet 3 can be separated from the cell crawling sheet 3 gripper.

To sum up, with the help of the above technical solutions of the present invention, the cell crawler 3 grasper of the present invention can easily and quickly grasp the cell crawler 3, reducing the crawling of the crawler due to improper operation when grasping the crawler. The probability of fragmentation provides convenience for carrying out related experiments; the present invention is simple in structure, convenient in operation, and more convenient for people to use and grasp the cell crawling slide 3 .

Through the above specific embodiments, those skilled in the art can easily implement the present invention. However, it should be understood that the present invention is not limited to the specific embodiments described above. On the basis of the disclosed embodiments, those skilled in the technical field can arbitrarily combine different technical features to realize different technical solutions.

Claims (6)

1. a kind of cell climbing sheet grabber, which is characterized in that described including hollow cylinder (1), push rod (2) and cell climbing sheet (3) Hollow cylinder (1) is internally connected with the push rod (2), and the push rod (2) and the hollow cylinder (1) dismantling connection, institute State the bottom end of hollow cylinder (1) diameter be less than the hollow cylinder (1) top diameter, and the hollow cylinder (1) Bottom end is pyramidal structure, and the bottom end of the hollow cylinder (1) is fixed with sucker (4), surface a side of the hollow cylinder (1) High-order limit hole (5) and low level limit hole (6) are offered, top a side of the hollow cylinder (1) is connected with by pressure arm (7), the interior bottom portion of the hollow cylinder (1) is fixed with reset spring (8), the bottom end of the push rod (2) be fixed with it is described The compatible rubber plug (9) of hollow cylinder (1), the bottom side side of the push rod (2) are connected with elastic spacing device (10), and described Elastic spacing device (10) is connect by tilting bar (11) with the push rod (2), the diameter of the elastic spacing device (10) with it is described The diameter of high-order limit hole (5) and the low level limit hole (6) is adapted.

2. a kind of cell climbing sheet grabber according to claim 1, which is characterized in that the high position limit hole (5), low level Limit hole (6) and the same side for being located at the hollow cylinder (1) by pressure arm (7), and be located in same horizontal line.

3. a kind of cell climbing sheet grabber according to claim 1, which is characterized in that the rubber plug (9) with it is described hollow Cylinder (1) interference fit, and the bottom diameter of the rubber plug (9) is less than the top end diameter of the rubber plug (9).

4. a kind of cell climbing sheet grabber according to claim 1, which is characterized in that the top of the push rod (2) is fixed Have pressing plate (12), and the pressing plate (12) is round pressing plate (12).

5. a kind of cell climbing sheet grabber according to claim 1, which is characterized in that the vertical height by pressure arm (7) Degree and the low level limit hole (6) are described by the bottom end of pressure arm (7) and close to the hollow cylinder (1) in same level A side be fixed with protrusion press surface.

6. a kind of cell climbing sheet grabber according to claim 1, which is characterized in that the sucker (4) is rubber material.