CN109867565A - A kind of plant protection product and its application method - Google Patents
A kind of plant protection product and its application method Download PDFInfo
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- CN109867565A CN109867565A CN201910209150.6A CN201910209150A CN109867565A CN 109867565 A CN109867565 A CN 109867565A CN 201910209150 A CN201910209150 A CN 201910209150A CN 109867565 A CN109867565 A CN 109867565A
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- 239000004476 plant protection product Substances 0.000 title description 17
- 238000000855 fermentation Methods 0.000 claims abstract description 38
- 230000004151 fermentation Effects 0.000 claims abstract description 38
- 241000187747 Streptomyces Species 0.000 claims abstract description 24
- 241000235646 Cyberlindnera jadinii Species 0.000 claims abstract description 23
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 22
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
本发明公开了一种植物保护剂及其使用方法，该植物保护剂包括以下成分：100～200重量份尿素和5～8重量份复合微生物菌剂，二者混匀后即得植物保护剂；所述复合微生物菌剂由产朊假丝酵母、解淀粉芽孢杆菌和泾阳链霉菌在发酵培养基中混合发酵而成。本发明通过尿素和复合微生物菌剂的协同作用，增强对害虫的刺激作用，有效驱逐害虫，同时又可引诱害虫天敌，具有有明显的害虫防治效果，虫口减退率达到70％～84％。对病害的防治作用也明显增强，椰茎干泻血病发病率仅为0～2％。The invention discloses a plant protection agent and a method for using the same. The plant protection agent comprises the following components: 100-200 parts by weight of urea and 5-8 parts by weight of a composite microbial inoculant, and the plant protection agent is obtained after the two are mixed evenly; The composite microbial inoculum is prepared by mixing and fermenting Candida utilis, Bacillus amyloliquefaciens and Streptomyces jingyang in a fermentation medium. Through the synergistic effect of urea and the compound microbial inoculant, the invention enhances the stimulating effect on pests, effectively expels the pests, and at the same time can attract the natural enemies of the pests, has obvious pest control effect, and the insect population reduction rate reaches 70% to 84%. The prevention and control of diseases is also significantly enhanced, and the incidence of copra stem diarrhea blood disease is only 0 to 2%.
Description
Technical field
The present invention relates to the techniques of pest control of agriculture and forestry plant more particularly to agriculture and forestry plant protection products.
Background technique
Currently, plant pest is still with chemical pesticide for main control measure.Chemical pesticide is used for a long time, pest is caused to produce
Different degrees of drug resistance is given birth to, control efficiency is remarkably decreased, and there is also pollution problems for chemical pesticide, cause to human health
It seriously affects.For solve chemical pesticide there are the drawbacks of, researcher further has developed biological pesticide, such as application number
The patent of invention of CN201310738242.6 discloses preparation method and the microorganism obtained of a kind of microbial manure or pesticide
Fertilizer or pesticide；By the way that peat slag is uniformly mixed with saccharomycete, actinomyces and bacillus, and with fiery numb seed slag, bean dregs and
The mixing of tobacco rod root, ferments under the action of peat immobilised enzymes, obtains medicine fertilizer intermediate product；Medicine fertilizer intermediate product is packed,
Continue to ferment, obtains microbial manure；Or, medicine fertilizer intermediate product, which is carried out engineering, expands bacterium, continue to ferment, it is filling, obtain microorganism
Liquid pesticide.It achieves preferable effect of increasing production, but diseases prevention and vermins-proof effect still need to further strengthen.
Summary of the invention
In consideration of it, the present invention provides a kind of plant protection product solution problem above.
The technical solution adopted by the present invention is as follows:
A kind of plant protection product, including following component: 100~200 parts by weight of urea and 5~8 parts by weight complex microorganisms
Microbial inoculum, up to plant protection product after the two mixing；The complex micro organism fungicide is by candida utili, bacillus amyloliquefaciens
Mixed fermentation forms in the fermentation medium with Streptomyces jingyangensis.
Preferably, fermentation temperature is 30~38 DEG C, and fermentation pH control is 7.0~7.5.
Preferably, fermentation medium is by 1000 parts by weight water, 10~25 parts by weight Corn powder, 10~25 parts by weight grapes
Sugar, 2~5 parts by weight sodium carbonate, 3~5 parts by weight of ferrous sulfate, 0.5~1 parts sulfuric acid magnesium and 0.1~0.2 parts by weight carbonic acid
Calcium composition.
The present invention furthermore provides the preparation method of complex micro organism fungicide:
A. prepare candida utili and bacillus amyloliquefaciens mixed bacteria liquid: picking candida utili be inoculated by
1000 parts by weight water, 0.5~0.8 parts by weight yeast powder, 15~20 parts by weight glucose, 3~5 parts by weight kelp ashes and 0.5~2
It is cultivated 1~2 day on the culture medium of parts sulfuric acid magnesium composition, 10~15 parts by weight peptones and 50~100 parts by weight chlorine is added
Change sodium, be then inoculated with bacillus amyloliquefaciens, continue culture 1~3 day, obtains candida utili and bacillus amyloliquefaciens are mixed
Combined bacteria liquid；
Candida utili content is (10~15) × 10 in mixed bacteria liquid9cfu/mL；Starch gemma is solved in mixed bacteria liquid
Bacillus content is (1~3) × 109cfu/mL；
B. prepare Streptomyces jingyangensis bacterium solution: picking Streptomyces jingyangensis is inoculated by 1000 parts by weight water, 15~20 parts by weight
It is cultivated 1~2 day on the culture medium of soluble starch, 0.5~1 parts by weight of potassium nitrate and 0.5~1 parts by weight of ferrous sulfate composition,
Obtain Streptomyces jingyangensis bacterium solution；Streptomyces jingyangensis content is (10~20) × 10 in bacterium solution9cfu/mL；Wherein, after inoculation also
It is passed through ozone；
C. it prepares complex microbial community: being respectively 0.3% according to weight percent by step A and step B gained bacterium solution
Inoculum concentration is inoculated into fermented and cultured in fermentation medium, obtains complex micro organism fungicide.
Preferably, ozone concentration is 10~15mg/m3, being passed through ozonation time is 20~30min.
Preferably, the plant protection product is used for babassu.
Preferably, by 100~200 times of the plant protection dilution agent, 5~10 jin are applied per acre.
Plant protection product of the present invention is forbidden mixed with other antibiotic, insecticide, fungicide, disinfectant, strong acid bases product
It closes and uses.
Plant protection product of the present invention is suitable for all growth phases of babassu, which is applied to soil
In.
Compared with prior art, the beneficial effects of the present invention are:
1, the study found that many pest natural enemies (parasitic wasp) carry out positioning parasitism to pest by urea, that is to say, that urine
Element is natural enemy attractant, and the present invention mainly traps pest natural enemy by urea to control pest.Diseases prevention is mainly by by production protein
Complex micro organism fungicide made of Candida, bacillus amyloliquefaciens and Streptomyces jingyangensis mixed fermentation is realized.Using a variety of
Profitable strain cooperates with chain reaction, generates a large amount of benefit materials easily absorbed for crop, such as amino acid, organic acid, polysaccharide, each
Kind vitamin, various biochemical enzymes, somatomedin, antibiotic and disease-resistant (poison) substance etc., build up fertility and holding effect power, raising are made
The immune function of object promotes healthy growth, improves soil, reduces the use of chemical fertilizer and pesticide；As it can be seen that the present invention can insect prevention,
Diseases prevention and enhancing healthy growth of crops, volume increase 10%, realize insect prevention, diseases prevention and the triple function for supplementing nutrient；In addition, of the invention
By the synergistic effect of urea and complex micro organism fungicide, there is stimulation, effectively expulsion pest to pest, while can draw again
The natural enemy of lepidoptera pest is lured, there is preferable insect prevention protection effect.
2, the present invention has found after furtheing investigate to the preparation method of composite bacteria agent, and processing method through the invention is compound
Microbial inoculum and urea synergistic effect are reinforced, obvious to the stimulation of pest, effectively expulsion pest, while can largely lure pest again
Natural enemy, there is apparent pest controling effect, and Revision insect recluced rate reaches 84%.The preventive and therapeutic effect of disease is also remarkably reinforced,
It is only 0~2% that coconut palm stem, which rushes down blood disease disease incidence,.
Specific embodiment
Below by specific embodiment, invention is further described in detail.
Candida utili, brewer's yeast described in the embodiment of the present invention, bacillus amyloliquefaciens, bacillus subtilis and river rising in Ningxia and flowing into central Shaanxi
Positive streptomycete is that market is bought.
Embodiment 1
A kind of plant protection product, including following component: 100 parts by weight of urea and 5 parts by weight complex micro organism fungicides；It is described
Complex micro organism fungicide is connect by candida utili bacterium solution, bacillus amyloliquefaciens bacterium solution and Streptomyces jingyangensis bacterium solution by 0.3%
Kind amount inoculation forms for mixed fermentation 2 days in the fermentation medium.Fermentation temperature is 30~38 DEG C, fermentation pH control 7.0~
7.5。
Fermentation medium by 1000 parts by weight water, 10 parts by weight Corn powder, 10 parts by weight glucose, 2 parts by weight sodium carbonate,
3 parts by weight of ferrous sulfate, 0.5 parts sulfuric acid magnesium and 0.1 weight parts of calcium carbonate composition.
Embodiment 2
A kind of plant protection product, including following component: 200 parts by weight of urea and 8 parts by weight complex micro organism fungicides；It is described
Complex micro organism fungicide is by the mixed fermentation in the fermentation medium of candida utili, bacillus amyloliquefaciens and Streptomyces jingyangensis
It forms within 2 days.Fermentation temperature is 30~38 DEG C, and fermentation pH control is 7.0~7.5.
Fermentation medium by 1000 parts by weight water, 25 parts by weight Corn powder, 25 parts by weight glucose, 5 parts by weight sodium carbonate,
5 parts by weight of ferrous sulfate, 1 parts sulfuric acid magnesium and 0.2 weight parts of calcium carbonate composition.
Embodiment 3
Embodiment 3 and the difference of embodiment 2 are:
The preparation method of complex micro organism fungicide:
A. prepare candida utili and bacillus amyloliquefaciens mixed bacteria liquid: picking candida utili be inoculated by
1000 parts by weight water, 0.5 parts by weight yeast powder, 15 parts by weight glucose, 3 parts by weight kelp ashes and 0.5 parts sulfuric acid magnesium group
At culture medium on cultivate 1 day, 10 parts by weight peptones and 50 parts by wt NaCl are added, then inoculation solution starch gemma bar
Bacterium continues culture 1 day, obtains candida utili and bacillus amyloliquefaciens mixed bacteria liquid；
Candida utili content is 10 × 10 in mixed bacteria liquid9cfu/mL；Bacillus amyloliquefaciens contain in mixed bacteria liquid
Amount is 1 × 109cfu/mL；
B. prepare Streptomyces jingyangensis bacterium solution: picking Streptomyces jingyangensis is inoculated into solvable by 1000 parts by weight water, 15 parts by weight
Property starch, 0.5 parts by weight of potassium nitrate and 0.5 parts by weight of ferrous sulfate composition culture medium on cultivate 1 day, obtain Streptomyces jingyangensis
Bacterium solution；Streptomyces jingyangensis content is 10 × 10 in bacterium solution9cfu/mL；Wherein, it is also passed through ozone after inoculation；Ozone concentration is
10mg/m3, being passed through ozonation time is 20min.
C. it prepares complex microbial community: being respectively 0.3% according to weight percent by step A and step B gained bacterium solution
Inoculum concentration is inoculated into fermented and cultured 2 days in fermentation medium, obtains complex micro organism fungicide.
Embodiment 4
Embodiment 4 and the difference of embodiment 2 are:
The preparation method of complex micro organism fungicide:
A. prepare candida utili and bacillus amyloliquefaciens mixed bacteria liquid: picking candida utili be inoculated by
1000 parts by weight water, 0.8 parts by weight yeast powder, 20 parts by weight glucose, 5 parts by weight kelp ashes and 2 parts sulfuric acid magnesium composition
Culture medium on cultivate 2 days, 15 parts by weight peptones and 100 parts by wt NaCl are added, are then inoculated with bacillus amyloliquefaciens,
Continue culture 3 days, obtains candida utili and bacillus amyloliquefaciens mixed bacteria liquid；
Candida utili content is 15 × 10 in mixed bacteria liquid9cfu/mL；Bacillus amyloliquefaciens contain in mixed bacteria liquid
Amount is 1 × 109cfu/mL；
B. prepare Streptomyces jingyangensis bacterium solution: picking Streptomyces jingyangensis is inoculated into solvable by 1000 parts by weight water, 20 parts by weight
Property starch, 1 parts by weight of potassium nitrate and 1 parts by weight of ferrous sulfate composition culture medium on cultivate 2 days, obtain Streptomyces jingyangensis bacterium
Liquid；Streptomyces jingyangensis content is 20 × 10 in bacterium solution9cfu/mL；Wherein, it is also passed through ozone after inoculation；Ozone concentration is
15mg/m3, being passed through ozonation time is 30min.
C. it prepares complex microbial community: being respectively 0.3% according to weight percent by step A and step B gained bacterium solution
Inoculum concentration is inoculated into fermented and cultured 2 days in fermentation medium, obtains complex micro organism fungicide.
Embodiment 5
Embodiment 5 and the difference of embodiment 4 are: sodium chloride is 30 parts by weight in step A.
Embodiment 6
Embodiment 6 and the difference of embodiment 4 are: ozone concentration is 5mg/m in step B3, being passed through ozonation time is 10min.
Comparative example 1:
A kind of plant protection product, including following component: 50 parts by weight of urea and 10 parts by weight complex micro organism fungicides；It is described
Complex micro organism fungicide is connect by candida utili bacterium solution, bacillus amyloliquefaciens bacterium solution and Streptomyces jingyangensis bacterium solution by 0.3%
Kind amount inoculation forms for mixed fermentation 2 days in the fermentation medium.Fermentation temperature is 30~38 DEG C, fermentation pH control 7.0~
7.5。
Fermentation medium by 1000 parts by weight water, 10 parts by weight Corn powder, 10 parts by weight glucose, 2 parts by weight sodium carbonate,
3 parts by weight of ferrous sulfate, 0.5 parts sulfuric acid magnesium and 0.1 weight parts of calcium carbonate composition.
Comparative example 2
A kind of plant protection product, including following component: 100 parts by weight of urea and 5 parts by weight complex micro organism fungicides；It is described
Complex micro organism fungicide is connect by brewer's yeast bacterium solution, bacillus subtilis bacterium solution and Streptomyces jingyangensis bacterium solution by 0.3% inoculum concentration
Kind forms for mixed fermentation 2 days in the fermentation medium.Fermentation temperature is 30~38 DEG C, and fermentation pH control is 7.0~7.5.
Fermentation medium by 1000 parts by weight water, 10 parts by weight Corn powder, 10 parts by weight glucose, 2 parts by weight sodium carbonate,
3 parts by weight of ferrous sulfate, 0.5 parts sulfuric acid magnesium and 0.1 weight parts of calcium carbonate composition.
Test example
The plant protection product of embodiment and comparative example is used for babassu (this test example selects coconut) respectively, will be planted
Object protects 100 times of dilution agent, applies 5 jin (at 200 times of dilution, amount of application is increase accordingly) per acre.Investigation woodland evil after application 7 days
Worm (by taking coconut knits moth as an example) and pest natural enemy quantity.It the results are shown in Table 1.
Plant protection product rushes down the control efficiency of blood disease to coconut coconut palm stem: coconut palm stem rushes down blood disease and mostly occurs in or so 20 ages
There is crack and flow out red juice in coconut, disease early period of origination, butt, gradually blackening later, and crack tissue rots, and
It is gradually extended up by base portion, serious Shi Guanye becomes smaller, and tree crown is wilting, falls off.This test selection example 1, embodiment 3, reality
The plant protection product of example 5 and comparative example is applied, is applied in 1 mu of palm grove respectively, application in 2 months is primary, sees whether out after 1 year
Existing coconut palm stem rushes down blood disease symptoms, to doubtful morbidity coco sample detection pathogen, calculates disease incidence.It the results are shown in Table 1.
Table 1
Verified, plant protection product of the present invention has certain stimulation to pest, can effectively expel pest, while again
The natural enemy of lepidoptera pest can be lured, there is apparent pest controling effect, Revision insect recluced rate reaches 70%~84%.This hair
Bright also to have apparent preventive and therapeutic effect to the disease of babassu, it is only 0~2% that coconut palm stem, which rushes down blood disease disease incidence,.
The above content is specific embodiment is combined, further detailed description of the invention, and it cannot be said that this hair
Bright specific implementation is only limited to these instructions.For those of ordinary skill in the art to which the present invention belongs, it is not taking off
Under the premise of from present inventive concept, a number of simple deductions or replacements can also be made.
Claims (8)
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