CN101270012B - Novel microorganism medicine fertilizer and preparation method thereof - Google Patents
Novel microorganism medicine fertilizer and preparation method thereof Download PDFInfo
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- CN101270012B CN101270012B CN2008100497146A CN200810049714A CN101270012B CN 101270012 B CN101270012 B CN 101270012B CN 2008100497146 A CN2008100497146 A CN 2008100497146A CN 200810049714 A CN200810049714 A CN 200810049714A CN 101270012 B CN101270012 B CN 101270012B
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Abstract
The present invention discloses a novel microbial medicine fertilizer in the technological field of microbiology and a preparation method thereof. The raw materials that are used for preparing the medicine fertilizer have the following portions: 1 portion of photosynthetic bacteria of 310,000,000 per milliliter, 1 portion of lactic acid bacteria of 420,000,000 per milliliter, 1 portion of acetobacter spp of 180,000,000 per milliliter, 1 portion of yeast of 200,000,000 per milliliter, 1 portion of Gram-positive actinomycete of 150,000,000 per milliliter, 1 portion of filamentous aspergillus of 200,000,000 per milliliter in the system of fermentation, 0.5 portion of rice vinegar, 0.5 portion of white wine, 0.015 portion of azadirachtin, 3 portions of salt and 390 portions of purified water. The novel microbial medicine fertilizer has the advantages of high activity, high stability and fast effect, and has the functions of killing harmful bacteria, killing pests and fertilization. And the microbial medicine fertilizer is non-toxic and conducive to the environmental protection. The preparation method has the advantages of simple process, easy operation, high practicality and suitability for the development of local areas, and fits the national conditions of China and the trend of agricultural development. And the technology has broad application prospects in agriculture, animal husbandry, aquaculture and environmental protection.
Description
Technical field
The present invention relates to a kind of biological medicine fertilizer technical field that fruit is dredged disease of preventing and treating, specifically belong to a kind of novel microorganism medicine fertilizer, also relate to the preparation method of this microorganism medicine fertilizer.
Background technology
Agricultural chemicals, chemical fertilizer are the requisite material used for agriculture of modern agriculture, when satisfying the demand of people to grain growth, have also paid high ecotope and Resources Consumption.In soil, has the chemical fixation effect as chemical fertilizer and chemical pesticide, excess is used adverse consequencess such as can causing soil compaction, soil acidity increase, simultaneously, must cause increasing the weight of of the face of land and groundwater pollution, how China's surface water body has been subjected to the pollution of nutritive substances such as nitrogen, phosphorus.Using chemical fertilizer, agricultural chemicals in a large number also causes many biotic population quantity sharply to reduce, even extinction, havoc the eubiosis, the long-term frequent chemical pesticide that uses can cause germ, insect pest resistance to occur, the drug effect of chemical pesticide itself is reduced gradually, just need to strengthen dosage for reaching the sterilization purpose.Like this, not only can improve agricultural cost, pesticide residue is increased sharply, very unfavorable to HUMAN HEALTH, serious environment pollution again.People have developed some biological pesticides after being familiar with the problems referred to above gradually, these biological pesticides have the better prevention ability, can also protect pest natural enemy, and are to the person poultry harmless, free from environmental pollution.But the biological pesticide sterilant of current domestic institute development and production is because it prevents and treats mechanism is that metabolite by microorganism active somatic cell self suppresses harmful microorganism, its preparation technology is to be purpose to extract viable bacteria in preparation process, and is the most just to extract in the viable bacteria amount.In practice process, find, shortcomings such as there is the control poor stability in the biological pesticide sterilant, action effect is slow, effect is single, and traditional microbial product is taked the production model of heavy industrialization, exist that middle-chain is many, active low and the disadvantage that cost is high of product, become restraining factors and the bottleneck on agricultural, applied.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, provide a kind of high reactivity, high stability, action effect fast, cost is low, novel microorganism medicine fertilizer with fertilising, sterilization, desinsection multi-efficiency.
Another object of the present invention provides the preparation method of this novel microorganism medicine fertilizer.
For achieving the above object, the technical solution used in the present invention is: a kind of novel microorganism medicine fertilizer is characterized in that the raw material for preparing this medicine fertilizer is by weight: 3.1 hundred million strains/1 part of milliliter photosynthetic bacterium, 4.2 hundred million strains/1 part of milliliter milk-acid bacteria, 1.8 hundred million strains/1 part of milliliter bacillus aceticus, 2.0 hundred million strains/1 part of ml yeast bacterium, 1.5 hundred million strains/1 part of milliliter Gram positive actinomycetes, 1 part in the thread aspergillus of 2.0 hundred million strains/milliliter fermentation system, 0.5 part of 5 degree rice vinegar, 38 0.5 part of liquor of degree, 0.015 part of nimbin, 3 parts of salt, 390 parts of pure water.
The preparation method of novel microorganism medicine fertilizer of the present invention:
1, in 0.5 ton of fermentor tank, adds 1. 1 part in the thread aspergillus that ferments and be by 3.1 hundred million strains/1 part of milliliter photosynthetic bacterium, 4.2 hundred million strains/1 part of milliliter milk-acid bacteria, 1.8 hundred million strains/1 part of milliliter bacillus aceticus, 2.0 hundred million strains/1 part of ml yeast bacterium, 1.5 hundred million strains/1 part of milliliter Gram positive actinomycetes, 2.0 hundred million strains/milliliter, the bacterium mixed solution of formation successively; 2. 5 degree rice vinegars are 0.5 part; 3. 38 degree liquor are 0.5 part; 4. nimbin is 0.015 part; 5. salt is 3 parts;
2, add 390 parts of pure water;
3, spend temperature bottom fermentations 360 hours 38;
4, be cooled to 26 degree, fermented 120 hours;
5, emit bacterium liquid, packaging final prod.
Adopt the beneficial effect of technique scheme: this novel microorganism medicine fertilizer contains photosynthetic bacterium, photosynthetic bacterium belongs to the autotrophy microorganism, thalline itself contains the protein more than 60%, and is rich in multivitamin, also contains Coenzyme Q10 99.0, antiviral substance and somatomedin; The light and heat that it is accepted with soil is the energy, and sulphur hydrogen in the soil and the hydrogen in the hydrocarbon polymer are separated, and the change objectionable impurities is innoxious substance.And be matrix with the secretory product of plant root, organism, obnoxious flavour (hydrogen sulfide etc.) and carbonic acid gas, ammonia etc. in the soil, synthetic carbohydrate, amino acids, vitamins, nitrogen compound, antiviral substance and physiologically active substance etc. are rich soil and the main strength that promotes animal and plant growth.The metabolic substd of photosynthetic bacteria group can directly be absorbed by plant, can also become the nutrient of other microbial reproduction.If photosynthetic bacterium propagation, other beneficial microorganism also can be bred.For example: VA mycorhiza bacterium is a bait with photosynthetic bacteria excretory amino acid, and it can dissolve insoluble phosphorus, again can with the vinelandii symbiosis, its nitrogen fixing capacity is significantly improved.This novel microorganism medicine fertilizer contains milk-acid bacteria, and milk-acid bacteria is by the picked-up photosynthetic bacterium, and the carbohydrate that yeast produces forms lactic acid.Lactic acid has very strong sterilizing ability, can effectively suppress the movable and organic rapid corrupt decomposition of harmful microbe.Milk-acid bacteria can be decomposed not labile under normal conditions xylogen and Mierocrystalline cellulose, and eliminates all drawbacks that undecomposed organism produces, and plays a significant role on organic matter fermentation decomposes.Milk-acid bacteria can also suppress the pathogenic bacterium propagation that continuous cropping obstacle produces.Pathogenic bacterium are active, and harmful nematode can sharply increase, and plant will be weak, and milk-acid bacteria has suppressed pathogenic bacterium, and harmful nematode just can fade away.This novel microorganism medicine fertilizer contains yeast, and secretory product, photosynthetic bacteria synthetic amino acid, carbohydrate and other organic substance that yeast utilizes plant root to produce produce fermenting power, synthetic root growth and the fissional sensitization material of promoting.Yeast is providing important subsistence support for promoting other beneficial microorganism (as milk-acid bacteria, actinomycetes) to breed needed matrix (food).This novel microorganism medicine fertilizer contains Gram positive actinomycetes, and Gram positive actinomycetes obtains amino acid from photosynthetic bacterium, and nitrogen etc. produce various antibiotics, VITAMIN and enzyme as matrix, can directly suppress pathogenic bacteria.It obtains harmful mould and the needed matrix of bacterial multiplication in advance, thereby suppresses their propagation, and creates the living environment of other beneficial microorganism propagation.The mixed clean bacterium effect of actinomycetes and photosynthetic bacterium is more much bigger than the lethality of actinomycetes individual combat.It has Degradation to the material that difficulty is decomposed as xylogen, Mierocrystalline cellulose, chitin etc., makes its easy passive plant absorbing, strengthens resistibility and the immunizing power of animals and plants to various diseases.Actinomycetes also can promote vinelandii and VA mycorhiza bacterium propagation.This novel microorganism medicine fertilizer contains the thread aspergillus of fermentation system, and the Eurotium that the thread aspergillus of fermentation system uses during with fermentation alcohol is main body, its can and other microorganisms coexistences, especially the generation to acetic acid in the soil has good result.This novel microorganism medicine fertilizer contains rice vinegar and alcohol; rice vinegar and alcohol are through reaction; particularly multiple microbial fermentation; produce the ester class, this product can strengthen plant metabolism, strengthens the stratum corneum of blade protection film; prevent that pathogenic bacteria from entering; its ester composition does not decompose in herbvore pest body, produces physiological barrier and causes death, and various pests such as nematode, aphid, looper, red spider, oriental tobacco budworm, tangerine a red-spotted lizard are had unusual effect.This novel microorganism medicine fertilizer contains nimbin, and after nimbin underwent microbial fermentation, insecticidal effect strengthened 15 times.Above-mentioned component cooperation has the stability height, action effect is fast; the three efficacy of kill harmful bacterium, kill pests, fertilising; and three kinds of effects are multiplied; reduce labour intensity greatly; three things are once finished, and raw material mainly is probiotic bacterium and frequently seen plants extract, and cost is low; environment is not only formed destruction, and also have very big provide protection.Preparation method's technology of this novel microorganism medicine fertilizer is simple, and the medicine fertilizer of preparation has high reactivity, and is easy to operate, practical, be fit to the localization development, tally with the national condition and the agricultural development direction, this technology has broad application prospects on agricultural, animal husbandry and fishery industry, environmental protection industry.
The fertile efficacy test of field medicine below in conjunction with test explanation novel microorganism medicine fertilizer of the present invention (hereinafter to be referred as ST-4).
One, prevention and elimination of disease and pests test
Catalogue
1, Chinese cabbage preventing and treating rot disease test
2, spinach root rot control test
Effect: multiple disease and pest is all had better prevention, and red spider, looper, heart-eating worm, cabbage heart worm, Peptic Ulcers, anthraenose all have special efficacy.
Characteristics: little property low toxicity, noresidue, low, the use wide spectrum of price.
(1) Chinese cabbage preventing and treating rot disease test
1, test period: in May, 2006, spray before at 7 o'clock in the morning or after at 4 o'clock in afternoon.
2, test site: oneself vegetable plot of seeds company.
3, prescription:
1: 400 times of ST-4, big-120 3.34 milliliters of fruit-vegetable type cloud of big-120 every spray one buckets (15 kilograms) of grain type cloud, mixing sprays, and fully sprays the leaf positive and negative.Heart stage spray 1-2 time is wherein wrapped in opening quotation phase, bag heart stage spray 2-3 time.
4, test effect: spray investigation in back 3 days, fallen ill stop the morbidity; Investigation in the 5th day, plant restore normal growth.
5, precaution
1, prevents and treats to put prevention first.Spray is 1 time when Chinese cabbage opening quotation growth, sprays when beginning to wrap the heart for the second time, and spray for the third time before the fast balling.
2, before 8 o'clock morning of opening quotation phase, the mist spray is saturating positive.
3, as wrapping the heart and Bao Xinqian after at 4 o'clock in afternoon, mist sprays the positive and behind of leaf.
(2) spinach root rot control test
One, test period: in June, 2006
Two, test site: the logical food market of Shenzhen ring
Three, spinach growth situation at that time: because of the ponding that rains day after day, low temperature causes the mashed decortication of root-rot, and the blade yellow is bordering on dead state.
Four, prescription: 1: 500 times of ST-4, big-120 (3.34 milliliters of per 15 kg water) of fruit-vegetable type cloud, indigo element (Shenzhen product foliage fertilizer) 0.2%.Mix spraying 2 times, spray spray in back 5 days for the first time for the second time.
Application effect: sprayed Fa Xingen, listing after 14 days back 5 days.Spray is not dead behind fine day more than 80%.
Two, pest control trial report
Catalogue
1, cabbage caterpillar control test
2, control of aphids test
3, twill pretty young woman at night prevents and treats test
4, big greenish brown hawk moth test
5, big caterpillar control test
6, citrus leaf pretty young woman at latent night prevents and treats test
7, citrus is embroidered tick control test
8, capsicum oriental tobacco budworm control test
9, citrus tangerine a red-spotted lizard control test
(1) cabbage caterpillar control test
Test one,
One, test period: on May 30th, 2006, afternoon, 4 o'clock Ban were fine cloudy.
Two, test site: seeds company
Three, prescription: 1: 500 times of ST-4,1: 1000 times in Bt0.2% A Wei aqua, mixing sprays.
Four, test-results: sprayed back 2 days, the cabbage caterpillar part is dead; Sprayed back 5 days, all dead.
Test two,
One, test period: on May 18th, 2006, on June 10th, 2006, about 7 o'clock mornings.
Two, test site: the institute of agricultural sciences
Three, prescription: 1: 250 times of ST-4, Bt0.2%, 1: 1000 times in A Wei aqua, 0.5% Hunan are produced " the big fertile king of China " and (are contained N, P
2O
5, K
2O 〉=30%, in, the trace element 22%), mixing sprays.
Four, test-results: very remarkable to large and small cabbage caterpillar preventive effect.Sprayed mortality ratio 100% back second day.Highly beneficial to large and small Chinese cabbage growth simultaneously, not only kill insect pest, and blade is glossy dark green, bright.The blue or green moth breeding of unmatched grass is very fast, many.
(2) control of aphids test
Test one,
One, test period: on June 15th, 2006, before at 8 o'clock in the morning.
Two, test site: the institute of agricultural sciences.
Three, prescription: 1: 250 times of ST-4.
Four, test-results: effective to the greenbug worm, undesirable to black aphid effect.Better to black aphid with 1: 100 efficiency-timed fruit.
Test two,
One, test period: on May 29th, 2006, at 5 o'clock in afternoon.
Two, test site: seeds company.
Three, prescription: 1: 500 times of ST-4, salt 0.1%, mixing sprays.
Four, test-results: sprayed back second day, green aphid is all dead.And disease there is prophylactic effect.
(3) prodenia litura control test
One, test period: on May 20th, 2006, about at 6 o'clock in afternoon, fine day.
Two, test site: the second group food market, Luohu District.
Three, prescription: 1: 300 times of ST-4,1: 1000 times in Bt0.2% A Wei aqua, mix and use.
Four, test-results: sprayed back 7 days, the prodenia litura mortality ratio is more than 90%.
(4), big greenish brown hawk moth control test
One, test period: on June 15th, 2006, about 7 o'clock mornings.
Two, test site: the second group food market, Luohu District.
Three, prescription: 1: 400 times of ST-4,1: 1000 times in Bt0.2% A Wei aqua, 0.2% potassium primary phosphate, mixing sprays.
Four, prevention effect: spray inspection in back 2 days, the mostly dizzy fan of insect was sprayed back 3 days, and is all dead.
(5) big caterpillar control test
One, test period: on June 3rd, 2006, afternoon, the 4:30 point sprayed.
Two, test site: the 3rd group food market, Luohu District.
Three, prescription: 1: 300 times of ST-4,1: 1000 times in Bt0.2% A Wei aqua, mixing sprays.
Four, prevention effect: spray back 2 days dizzy fans; Spray exhausted most death in back 3 days, only a few is not seen corpse, doubts missing.
Five, precaution: the honeybee class is is easily killed and wounded this insecticidal solution sensitivity, and can not spray flowering period, in order to avoid the influence pollination causes the underproduction.
(6) leaf miner such as citrus control test
One, the time: on June 4th, 2006, spray about 7 o'clock mornings.
Two, place: orchard, South Mountain.
Three, prescription: 45 kilograms, 1: 200 times of clean water of mu
1: 1000 times in Bt0.2% A Wei aqua, 0.5% Hunan are produced " the big fertile king of China " and (are contained N, P
2O
5, K
2O 〉=30%, in, the trace element 22%), mixing sprays.
Four, effect: spray investigation in back second day, prevention effect reaches 100%.The blue or green moth of pest natural enemy grass is protected, and breeding is fast.
Five, precaution: can not spray when blooming; 45 kilograms of mu spray mixed solutions.
(7) citrus is embroidered tick control test
One, test period: citrus spring in 2006, summer be the phase slightly.Spray before 8 o'clock mornings or after at 4 o'clock in afternoon.
Two, test site: institute of agricultural sciences orchard
Three, prescription: 1: 500 times of ST-4, clean fresh potato powder 0.5% (take by weighing potato powder 250 grams before the use earlier, with 2.5 kilograms of furnishing pasty states of well water, after add 12.5 kilograms of boiling water and smash into thin liquid).Use 1: 500 times of ST-4 at last
=Pour fully evenly back use of puddle in 15 kilograms of potato powder thin liquids into for 30 milliliters.
Four, prevention effect: mortality ratio 100%.
Five, precaution
(1) mist evenly sprays leaf, stem, positive and negative, especially will spray the leaf reverse side.
(2) make the water must be with clean pure well water, river, open grave, ditch Zhen Shui.If use tap water, must park after 24 hours and use.
(3) about fine 7 o'clock mornings or spray about at 5 o'clock in afternoon.But spray all day at cloudy day.
(8) capsicum oriental tobacco budworm control test
One, test period: in early June, 2006.
Two, test site: capsicum field, the second group food market, Luohu District.
Three, prescription: 1: 400 times of ST-4, grain type cloud big-120 1: 100 times (per 1 kg water with big-1201 milliliters of cloud), mixing sprays.
Four, test-results; Spray after at 4 o'clock in afternoon, in second day capsicum all larvas climb out of that capsicum is outer, on the stem, leaf; Dead after 2-3 days.
Five, precaution
1, spray after at 4 o'clock in afternoon, best to spray at dusk effect.
2, mist fully sprays leaf, stem, pepper fruit pros and cons, till dripping.
(9) citrus tangerine a red-spotted lizard control test
One, test period: in May, 2006.
Two, test site: western Li Ganjuyuan.
Three, " the big fertile king of China " foliage fertilizer is produced in 1: 300 times of ST-4, big-120 (3.34 milliliters of per 15 kg water) of fruit tree type cloud, Hunan, and mixing sprays.
Four, test effect: spray back the 3rd day, tangerine a red-spotted lizard mortality ratio is 100%.
Five, precaution: can not spray when citrus is bloomed, in order to avoid kill and wound the honeybee class.
Three, ST-4 manurial experiment report
Catalogue
1, rice sprouts test early
2, late rice rice shoot test
3, ST-4 does the test of vegetables base manure
4, the root of kudzu vine is used test
(1) rice sprouts (field) test early
One, test period: rice sprouts (field) early in 2006.
Two, test site: the continuous contracted field of Luohu District second Li De of group.
Three, test design:
(1) kind: hybrid early " is assisted excellent No. 2 ".
(2) rice seedling bed soil fertility: medium, other slightly.
(3) sowing time: the morning on May 28th, 2006.
(4) spraying method: single spray, mixed spray, contrast (CK)
(5) spray period and number of times: one heart stage an of leaf, for the first time; Transplant spray in preceding 8 days for the second time.All after at 4 o'clock in afternoon, sprayed.
(6) spraying method: mist spray, spray leaf front.
(7) prescription: 45 kilograms in clear water in the ditch ditch between fields+
=56.70 milliliters of (closing 1.13 liang)+grain type clouds of ST-4 mix use for big-120,10 milliliters.
Four, compound method: earlier ST-4, water were diluted by 1: 10.Be 5 kilograms in well water, 56.7 milliliters of mixing of ST-4, pour at last in the plastics kettle or bucket of lid that sealing and fermenting was used after 7 days.During the fermentation, shake every day or puddle 1-2 time, wait foam to lose substantially after sealing and fermenting again, have sweetened vinegar tart flavour to get final product during use.Get at last 40 kilograms in clear water (ditch, open grave, river, well water)+
=5 kilograms+cloud of ST-4 mixes use for big-12010 milliliters.
Five, using method:
Spray (one heart stage an of leaf) for the first time: 22.5 kilograms of mixed solutions of mu, mist is hurried up and is sprayed.
Spray (transplanting preceding 8 days) for the second time: 45 kilograms of mixed solutions of mu, mist is careful and is sprayed.
Six, test-results
The spray back is observed for the first time, and growth potential strengthens, and leafing is fast, and leaf is green, the bright enhancing of cold hardiness etc.
Spray back observation survey result such as following table for the second time
Annotate: spray investigation in back 7 days, 100 strains of sampling radical.
(2) late rice rice shoot test
One, test period: 2006 6
Two, testing program;
(1) experimental cultivar: high-quality hybrid rice " Jin You osmanthus 99 ".
(2) for fertile (agent): the ST-4 of examination spray, cloud big by-120 (Yunnan University's development, rape lactone element, grain type).
(3) experimental plot fertility and fertilising situation
Red sand earth.Organic matter 1.19%, full N0.08%, alkaline hydrolysis N76.3ppm, available P
2O
51.1ppm, quick-acting K
2O ppm.PH5.27。
(4) rice seedling bed fertilising situation: mu is executed the Guixi product and is executed 62.5 kilograms of big strong composite fertilizers.Do base manure disposable executing down before ploughing a field.
(5) land for growing field crops fertilising situation: 11.2 kilograms in mu urea, 44 kilograms in calcium magnesium phosphorus, 5.5 kilograms of potassium primary phosphates, 1 kilogram in zinc sulfate.Do base manure disposable executing down before ploughing a field.
(6) test design
1, establishes three processing
(1) the seeding stage spray once (is called for short spray once);
(2) seeding stage, main fringe, each spray of fringe phase beginning once (are called for short spray 2 times);
(3) seeding stage, main fringe, fringe phase beginning, each spray of full heading time once (are called for short 3 times).
2,9 sub-districts, totally 27 sub-districts are established in each processing.Each sub-district triplicate.
3, spraying method: single spray ST-4 rice shoot (field) phase is sprayed 1: 800 times, 1: 400 times of field period spray, and big-120 seeding stages of cloud and field period all spray 1: 4500 times.Mix spray rice shoot (field), mu is with big-12010 milliliters of ST-4 56.7 milliliters (about 1.13 liang), cloud, and the open grave of cleaning, 45 kilograms of mixing of river ditch between fields water spray.Mix to spray the land for growing field crops seedlings of cereal crops, mu with big-120 10 milliliters of ST-4 112.5 milliliters (about 2.25 liang), cloud (with the rice seedling bed with).The contrast spray is with amount quantity clear water.
Three, test-results and analysis
(1) the plain investigation of rice shoot
The every quality situation of table 1 hundred strain rice shoots
Annotate: 1, sampling survey 100 strain roots.
2, every data are 100 mean numbers in the table.
3, transplant the condition survey of tillering the day before yesterday.
(2) it is in full accord respectively to handle breeding time.
(3) species test and output situation
Annotate: every processing species test 10 root and stem of certain plants.
(3) ST-4 does the test of vegetables base manure
One, the time: 5-7 month in 2006.
Two, place: Nanshan District vegetables ground.
Three, prescription; 2 kilograms of ST-4 of mu, 10 kilograms of dilutions of ditch ditch between fields clear water.After pour in cow dung and the human excrement crypt fermentation into after seven days, take the circumstances into consideration water mixing and make base manure and use.
Four, test-results: effect is obvious especially, and various vegetable growth gesture are prosperous, the glossy dark green light of blade.
Especially good especially, grey, good-looking to dome-type cauliflower, fresh kidney beans etc., the people who buys is many especially.In addition, ground, subterranean pest-insect and disease evil are obviously reduced.
Five, precaution: enrich 20% phosphate fertilizer and 30% potash fertilizer is used.
(4) root of kudzu vine is used test
One, test period: 2006.
Two, test site: Nanshan District second group.
Three, test design
(1) kind: wood is given birth to the root of kudzu vine
(2) soil fertility: red soil mountain region.Mu is executed 9000 kilograms on turf, 50 kilograms in calcium magnesium phosphorus.
(3) the plantation time: afternoon on March 26th, 2006
(4) spray scheme
1, the 2-3 leaf phase: spray big-120 per 15 kg water of fruit-vegetable type cloud and add 3.34 milliliters, 0.2% Hunan product " China is big fertile ", mixing sprays.
2, peak of growing season 6, July, 1: 500 times of ST-4 of spray in every month, 3.34 milliliters of big-120 per 15 kg water of fruit-vegetable type cloud, " China is big fertile " produced in 0.2% Hunan, and mixing sprays.
3, big phase 8 of piece root Peng, September, 1: 500 times of ST-4 of spray in every month, fruit-vegetable type cloud big-120 are held 3.34 milliliters of 15 kg water, 0.3% potassium primary phosphate, and mixing sprays.
Four, application effect: planted back 1 year, 5 root and stem of certain plants mean yields of spray are 17.8 kilograms, and Pen the average product of 5 strains is not 10.2 kilograms.The growth of spray is vigorous always, leaf look viridescent, and when more than 40 day autumn in summer was arid in 2006, leaf roll few (about 20%).The growth of not executing is general, and the leaf look light green, when arid, mostly (80%-90%) leaf roll.
Five, precaution: enrich 20% phosphate fertilizer and 30% nitrogen fertilizer amount.
From above-mentioned test as can be seen, novel microorganism medicine fertilizer of the present invention has the stability height, action effect is fast, the three efficacy of kill harmful bacterium, kill pests, fertilising.
Embodiment
Embodiment 1
The raw material for preparing this medicine fertilizer is by weight: 3.1 hundred million strains/1 kilogram of milliliter photosynthetic bacterium, 4.2 hundred million strains/1 kilogram of milliliter milk-acid bacteria, 1.8 hundred million strains/1 kilogram of milliliter bacillus aceticus, 2.0 hundred million strains/1 kilogram of ml yeast bacterium, 1.5 hundred million strains/1 kilogram of milliliter Gram positive actinomycetes, 1 kilogram in the thread aspergillus of 2.0 hundred million strains/milliliter fermentation system, 0.5 kilogram of 5 degree rice vinegar, 38 0.5 kilogram of liquor of degree, 0.015 kilogram of nimbin, 3 kilograms of salt, 390 kilograms of pure water.Above-mentioned microbial strains all can buy on market, is mixed with above-mentioned desired concn according to ordinary method.
The preparation method of this novel microorganism medicine fertilizer:
1, in 0.5 ton of fermentor tank, adds successively
1. by 1 kilogram of bacterium mixed solution that constitutes of thread aspergillus of 3.1 hundred million strains/1 kilogram of milliliter photosynthetic bacterium, 4.2 hundred million strains/1 kilogram of milliliter milk-acid bacteria, 1.8 hundred million strains/1 kilogram of milliliter bacillus aceticus, 2.0 hundred million strains/1 kilogram of ml yeast bacterium, 1.5 hundred million strains/1 kilogram of milliliter Gram positive actinomycetes, 2.0 hundred million strains/milliliter fermentation system;
2. 5 degree rice vinegars are 0.5 kilogram;
3. 38 degree liquor are 0.5 kilogram;
4. nimbin is 0.015 kilogram;
5. salt is 3 kilograms;
2, add 390 kilograms of pure water;
3, spend temperature bottom fermentations 360 hours 38;
4, be cooled to 26 degree, fermented 120 hours;
5, emit bacterium liquid, packaging final prod.
Claims (2)
1. a novel microorganism medicine fertilizer is characterized in that the raw material for preparing this medicine fertilizer is by weight: 3.1 hundred million strains/1 part of milliliter photosynthetic bacterium, 4.2 hundred million strains/1 part of milliliter milk-acid bacteria, 1.8 hundred million strains/1 part of milliliter bacillus aceticus, 2.0 hundred million strains/1 part of ml yeast bacterium, 1.5 hundred million strains/1 part of milliliter Gram positive actinomycetes, 1 part in the thread aspergillus of 2.0 hundred million strains/milliliter fermentation system, 0.5 part of 5 degree rice vinegar, 38 0.5 part of liquor of degree, 0.015 part of nimbin, 3 parts of salt, 390 parts of pure water.
2. the preparation method of novel microorganism medicine fertilizer according to claim 1:
(1), in 0.5 ton of fermentor tank, add successively:
1. by 1 portion of bacterium mixed solution that constitutes of thread aspergillus of 3.1 hundred million strains/1 part of milliliter photosynthetic bacterium, 4.2 hundred million strains/1 part of milliliter milk-acid bacteria, 1.8 hundred million strains/1 part of milliliter bacillus aceticus, 2.0 hundred million strains/1 part of ml yeast bacterium, 1.5 hundred million strains/1 part of milliliter Gram positive actinomycetes, 2.0 hundred million strains/milliliter fermentation system;
2. 5 degree rice vinegars are 0.5 part;
3. 38 degree liquor are 0.5 part;
4. nimbin is 0.015 part;
5. salt is 3 parts;
(2), add 390 parts of pure water;
(3), spend temperature bottom fermentations 360 hours 38;
(4), be cooled to 26 the degree, fermented 120 hours;
(5), emit bacterium liquid, packaging final prod.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100497146A CN101270012B (en) | 2008-05-03 | 2008-05-03 | Novel microorganism medicine fertilizer and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100497146A CN101270012B (en) | 2008-05-03 | 2008-05-03 | Novel microorganism medicine fertilizer and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101270012A CN101270012A (en) | 2008-09-24 |
| CN101270012B true CN101270012B (en) | 2011-04-13 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102503695A (en) * | 2011-11-09 | 2012-06-20 | 赵万群 | Microorganism bacterial fertilizer and preparation method thereof |
| CN105084998A (en) * | 2014-05-19 | 2015-11-25 | 段一皋 | Biological disinfector for soil environment and preparation method thereof |
| CN106316499A (en) * | 2015-06-25 | 2017-01-11 | 山东梓耘生态肥业有限公司 | Biological organic fertilizer |
| CN105724465A (en) * | 2016-03-29 | 2016-07-06 | 黑龙江北方孝道源农业科技发展有限公司 | Plant insect-proof and growth-promoting liquid |
| CN105948838B (en) * | 2016-04-26 | 2019-05-10 | 北京科拓恒通生物技术股份有限公司 | A kind of compound lactobacillus microbial manure and its preparation and application |
| CN106946625A (en) * | 2017-03-02 | 2017-07-14 | 湖北益丰生物肥业有限公司 | Paddy rice medication fertilizer and the application process of the fertile medicine integration of paddy rice |
| CN106927948A (en) * | 2017-03-02 | 2017-07-07 | 湖北益丰生物肥业有限公司 | A kind of medicine fertilizer and its application |
| CN106883018A (en) * | 2017-03-02 | 2017-06-23 | 湖北益丰生物肥业有限公司 | A kind of microbial manure and its application method for preventing and treating crop pest |
| CN106946602A (en) * | 2017-03-02 | 2017-07-14 | 湖北益丰生物肥业有限公司 | A kind of medicine fertilizer and its preparation, application method |
| CN107043728A (en) * | 2017-06-16 | 2017-08-15 | 云南优乳生物技术有限公司 | A kind of composite bacteria agent and method for preventing and treating powdery mildew of strawberry |
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| JP2002080290A (en) * | 2000-09-08 | 2002-03-19 | Nippon Kankeru Kk | Granulated fertilizer |
| CN1533696A (en) * | 2003-03-28 | 2004-10-06 | 吴永常 | Plant biological source disease and pest control liquid preparation (Green biological TC liquid preparation) |
| CN101050423A (en) * | 2007-03-19 | 2007-10-10 | 上海真美科技发展有限公司 | Composite preparation microbiological, and preparation method |
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| CN1189086A (en) * | 1996-12-03 | 1998-07-29 | 株式会社石井工作研究所 | IC terminal frame processing system |
| JP2002080290A (en) * | 2000-09-08 | 2002-03-19 | Nippon Kankeru Kk | Granulated fertilizer |
| CN1533696A (en) * | 2003-03-28 | 2004-10-06 | 吴永常 | Plant biological source disease and pest control liquid preparation (Green biological TC liquid preparation) |
| CN101050423A (en) * | 2007-03-19 | 2007-10-10 | 上海真美科技发展有限公司 | Composite preparation microbiological, and preparation method |
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