CN109618990A - A kind of incubation fluid and its hatching method of high density shelling slag oxygenation - Google Patents
A kind of incubation fluid and its hatching method of high density shelling slag oxygenation Download PDFInfo
- Publication number
- CN109618990A CN109618990A CN201811474417.6A CN201811474417A CN109618990A CN 109618990 A CN109618990 A CN 109618990A CN 201811474417 A CN201811474417 A CN 201811474417A CN 109618990 A CN109618990 A CN 109618990A
- Authority
- CN
- China
- Prior art keywords
- high density
- slag oxygenation
- hatching
- shelling slag
- shelling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000012447 hatching Effects 0.000 title claims abstract description 54
- 238000006213 oxygenation reaction Methods 0.000 title claims abstract description 43
- 239000002893 slag Substances 0.000 title claims abstract description 43
- 238000011534 incubation Methods 0.000 title claims abstract description 40
- 239000012530 fluid Substances 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 18
- 238000009938 salting Methods 0.000 claims abstract description 11
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 5
- 238000005286 illumination Methods 0.000 claims description 5
- 229910052760 oxygen Inorganic materials 0.000 claims description 5
- 239000001301 oxygen Substances 0.000 claims description 5
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 4
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 2
- 241000238582 Artemia Species 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 235000013601 eggs Nutrition 0.000 description 3
- 239000013535 sea water Substances 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- 241000143060 Americamysis bahia Species 0.000 description 2
- 241000133262 Nauplius Species 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000238426 Anostraca Species 0.000 description 1
- 241000595940 Notostraca Species 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000005058 diapause Effects 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention provides a kind of incubation fluids of high density shelling slag oxygenation, the salting liquid for being 19-22 ‰ including salinity, and the salting liquid that pH is 8-9 includes the Vc that concentration is 200-1000mg/L.The incubation fluid and its hatching method of a kind of high density shelling slag oxygenation of the present invention solve the problem of the low wasting of resources of shelling slag oxygenation hatching efficiency in the prior art, realize that shelling slag oxygenation reaches high hatching rate under high density.
Description
Technical field
The invention belongs to shelling slag oxygenation hatching technique fields, more particularly, to a kind of hatching of high density shelling slag oxygenation
Liquid and its method.
Background technique
Artemia (Brine shrimp, Artemia), is commonly called as fairy shrimp, be it is a kind of live in it is small-sized in highrank fuel salinity water domain
Shellfish, the nauplius for hatching the artemia of 1~2d have a large amount of yolk, and protein rich in, fat, are fishes and shrimps
The good open-mouthed bait of the young such as crab can greatly improve fishes and shrimps artificial breeding survival rate, not pollute water quality but.Common laboratory is real
The process hatching density of testing can achieve 3g/L, and generally hatch that field condition is limited, and hatching density can only achieve 1.7g/L or so.?
When hatching rate is identical, so low hatching density causes the hatching efficiency of shelling slag oxygenation low, resource and production space waste,
Cost is caused to increase, application of the nauplius as open-mouthed bait of this strong influence.
It would therefore be highly desirable to research and develop a kind of incubation fluid and hatching method, improved on the basis of guaranteeing shelling slag oxygenation hatching rate
The hatching density of shelling slag oxygenation improves the hatching efficiency of shelling slag oxygenation.
Summary of the invention
In view of this, the present invention is directed to propose a kind of incubation fluid and its hatching method of high density shelling slag oxygenation, solve
The problem of shelling slag oxygenation hatching efficiency is low in the prior art, the wasting of resources realizes that shelling slag oxygenation reaches high under high density
Hatching rate.
In order to achieve the above objectives, the technical scheme of the present invention is realized as follows:
A kind of incubation fluid of high density shelling slag oxygenation, the salting liquid for being 19-22 ‰ including salinity, pH are the salt of 8-9
Solution includes the Vc that concentration is 200-1000mg/L.
Preferably, the salting liquid for being 20-22 ‰ including salinity, the salting liquid that pH is 8-9 includes that concentration is 500-
The Vc of 800mg/L.
It preferably, include wherein aqueous slkali in salting liquid to adjust pH, aqueous slkali include that 0.3-1g/L sodium hydroxide is molten
Liquid or 1-3g/L sodium bicarbonate solution.
Preferably, high density shelling slag oxygenation is hatched in the incubation fluid, high density shelling slag oxygenation it is close
Spend 10-15g/L；White light, intensity of illumination 500-1500Lux；The solubility of dissolved oxygen is greater than 6mg/L, 26-29 DEG C of temperature；
Incubation fluid remains that pH is 8-9.
Preferably, using fed-batch mode to add aqueous slkali in real time keeps incubation fluid pH for 8-9.
Preferably, brooding time is less than for 24 hours.
Preferably, hatching rate is more than or equal to 90%.
Preferably, the addition manner of Vc is to be directly added into incubation fluid or be wrapped in be added to hatching in shelling slag oxygenation
In liquid.
Compared with the existing technology, incubation fluid and its hatching method tool of a kind of high density shelling slag oxygenation of the present invention
There is following advantage:
(1) the targeted Object of Development of the present invention is shelling slag oxygenation, solves the problems, such as that high density hatching hatching rate is low,
Realize diapause shelling slag oxygenation under the high density of 10-15g/L.
(2) present invention is under the high density of 10-15g/L, and hatching rate reaches 90% or more in 24 hours, hatching density
Greatly, hatching rate is high, and hatching efficiency is high.
(3) in incubation fluid of the present invention, addition Vc is water conditioner, improves incubation fluid stability.
(4) there are vital influences on the hatching of shelling slag oxygenation by pH in incubation fluid, with the progress of hatching, hatching
PH value declines in liquid, and faster with the speed for increasing pH decline of hatching density, and high density hatching is incubated in pH decline
Rate influence is very big, so the pH value that the present invention in such a way that stream adds, controls incubation fluid solubility in entire hatching process is kept
It between 8-9, realizes and quickly hatches under high density, and hatching rate is high.
Specific embodiment
In addition to being defined, technical term used in following embodiment has universal with those skilled in the art of the invention
The identical meanings of understanding.Test reagent used in following embodiment is unless otherwise specified conventional biochemical reagent；It is described
Experimental method is unless otherwise specified conventional method.
Below with reference to embodiment, the present invention will be described in detail.
Embodiment 1
Hatched using 300L incubation tank, 250L incubation fluid in hatching pipe is hatched.High density shelling slag oxygenation
Hatching density is 10g/L, and shelling slag oxygenation selects Kazakhstan's shelling slag oxygenation.
Incubation fluid includes that tap water is mixed with artificial seawater, wherein salinity 20 ‰；Adding Vc and adjusting concentration is 500mg/L,
0.4g/L sodium hydroxide solution is added, incubation fluid pH value 8.5 is made；Inflation makes the concentration > 6mg/L of dissolved oxygen；It 29 DEG C of water temperature, incubates
A direct current adds 0.4g/L sodium hydroxide solution during change, keeps the pH value of incubation fluid 8.5 or so.With white on incubation fluid liquid level
Light irradiation, and intensity of illumination is 1000lux.
After hatching 24 hours, the hatching rate of artemia eggs reaches 91%.
Embodiment 2
Hatched using 300L incubation tank, 250L incubation fluid in hatching pipe is hatched.High density shelling slag oxygenation
Hatching density is 15g/L, and shelling slag oxygenation selects Kazakhstan's shelling slag oxygenation.
Incubation fluid includes that tap water is mixed with artificial seawater, wherein salinity 20 ‰；Adding Vc and adjusting concentration is 800mg/L,
0.4g/L sodium hydroxide solution is added, incubation fluid pH value 8.5 is made；Inflation makes the concentration > 6mg/L of dissolved oxygen；It 29 DEG C of water temperature, incubates
A direct current adds 0.4g/L sodium hydroxide solution during change, keeps the pH value of incubation fluid 8.5 or so.With white on incubation fluid liquid level
Light irradiation, and intensity of illumination is 1000lux.
After hatching 24 hours, the hatching rate of artemia eggs reaches 91%.
Reference examples
Hatched using 300L incubation tank, 250L incubation fluid in hatching pipe is hatched.High density shelling slag oxygenation
Hatching density is 10g/L, and shelling slag oxygenation selects Kazakhstan's shelling slag oxygenation.
Incubation fluid includes that tap water is mixed with artificial seawater, wherein salinity 20 ‰；Adding Vc and adjusting concentration is 500mg/L,
Inflation makes the concentration > 6mg/L of dissolved oxygen；29 DEG C of water temperature, white light is used on incubation fluid liquid level, and intensity of illumination is
1000lux。
After hatching 24 hours, the hatching rate of artemia eggs reaches 35%.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (8)
1. a kind of incubation fluid of high density shelling slag oxygenation, it is characterised in that: the salting liquid for being 19-22 ‰ including salinity, pH
Salting liquid for 8-9 includes the Vc that concentration is 200-1000mg/L.
2. a kind of incubation fluid of high density shelling slag oxygenation according to claim 1, it is characterised in that: be including salinity
The salting liquid of 20-22 ‰, the salting liquid that pH is 8-9 include the Vc that concentration is 500-800mg/L.
3. a kind of incubation fluid of high density shelling slag oxygenation according to claim 2, it is characterised in that: wherein in salting liquid
It to adjust pH, aqueous slkali include 0.3-1g/L sodium hydroxide solution or 1-3g/L sodium bicarbonate solution including aqueous slkali.
4. a kind of hatching method of high density shelling slag oxygenation, it is characterised in that: high density shelling slag oxygenation is in such as claim
Hatched in the described in any item incubation fluids of 1-3, the density 10-15g/L of high density shelling slag oxygenation；White light, illumination
Intensity is 500-1500Lux；The solubility of dissolved oxygen is greater than 6mg/L, 26-29 DEG C of temperature；Incubation fluid remains that pH is 8-9.
5. a kind of hatching method of high density shelling slag oxygenation as claimed in claim 4, it is characterised in that: real using fed-batch mode
It is 8-9 that Shi Tianjia aqueous slkali, which keeps incubation fluid pH,.
6. a kind of hatching method of high density shelling slag oxygenation according to claim 4, it is characterised in that: brooding time is small
In for 24 hours.
7. a kind of hatching method of high density shelling slag oxygenation according to claim 4, it is characterised in that: hatching rate is big
In equal to 90%.
8. a kind of hatching method of high density shelling slag oxygenation according to claim 4, it is characterised in that: the addition side of Vc
Formula is to be directly added into incubation fluid or be wrapped in shelling slag oxygenation to be added in incubation fluid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811474417.6A CN109618990A (en) | 2018-12-04 | 2018-12-04 | A kind of incubation fluid and its hatching method of high density shelling slag oxygenation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811474417.6A CN109618990A (en) | 2018-12-04 | 2018-12-04 | A kind of incubation fluid and its hatching method of high density shelling slag oxygenation |
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| Publication Number | Publication Date |
|---|---|
| CN109618990A true CN109618990A (en) | 2019-04-16 |
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ID=66070859
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811474417.6A Pending CN109618990A (en) | 2018-12-04 | 2018-12-04 | A kind of incubation fluid and its hatching method of high density shelling slag oxygenation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113615610A (en) * | 2021-07-29 | 2021-11-09 | 天津海友佳音生物科技股份有限公司 | Hatching method for improving hatchability of artemia cysts |
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|---|---|---|---|---|
| WO2002028173A1 (en) * | 2000-10-05 | 2002-04-11 | Inve Technologies N.V. | Method for producing free swimming artemia nauplii and packaged cysts for use in that method |
| KR101333901B1 (en) * | 2005-09-26 | 2013-11-27 | 인베 테크놀로지즈 엔.브이. | Method to enhance hatching percentage of Artemia diapauzing cysts |
| CN104273099A (en) * | 2014-10-21 | 2015-01-14 | 天津海友佳音生物科技股份有限公司 | Method for increasing eclosion rate of diapause artermia eggs at high temperature |
| CN104488828A (en) * | 2014-12-18 | 2015-04-08 | 天津海友佳音生物科技股份有限公司 | A method for improving the hatching rate of artemia cysts after shelling |
| CN105941334A (en) * | 2016-04-29 | 2016-09-21 | 浙江省海洋水产研究所 | Method for incubation and rapid separation of Artemia nauplii |
| CN106719442A (en) * | 2016-11-29 | 2017-05-31 | 天津农学院 | A kind of method that man-made drugs' control produces Artemia cysts |
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-
2018
- 2018-12-04 CN CN201811474417.6A patent/CN109618990A/en active Pending
Patent Citations (7)
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|---|---|---|---|---|
| WO2002028173A1 (en) * | 2000-10-05 | 2002-04-11 | Inve Technologies N.V. | Method for producing free swimming artemia nauplii and packaged cysts for use in that method |
| KR101333901B1 (en) * | 2005-09-26 | 2013-11-27 | 인베 테크놀로지즈 엔.브이. | Method to enhance hatching percentage of Artemia diapauzing cysts |
| CN104273099A (en) * | 2014-10-21 | 2015-01-14 | 天津海友佳音生物科技股份有限公司 | Method for increasing eclosion rate of diapause artermia eggs at high temperature |
| CN104488828A (en) * | 2014-12-18 | 2015-04-08 | 天津海友佳音生物科技股份有限公司 | A method for improving the hatching rate of artemia cysts after shelling |
| CN105941334A (en) * | 2016-04-29 | 2016-09-21 | 浙江省海洋水产研究所 | Method for incubation and rapid separation of Artemia nauplii |
| CN106719442A (en) * | 2016-11-29 | 2017-05-31 | 天津农学院 | A kind of method that man-made drugs' control produces Artemia cysts |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113615610A (en) * | 2021-07-29 | 2021-11-09 | 天津海友佳音生物科技股份有限公司 | Hatching method for improving hatchability of artemia cysts |
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Application publication date: 20190416 |
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